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Artykuły w czasopismach na temat "Two time lapse video microscopy"

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Peippo, Jaana, and Peter Bredbacka. "A simple culture system for time-lapse video recording of bovine embryos." Agricultural and Food Science 5, no. 5 (1996): 515–20. http://dx.doi.org/10.23986/afsci.72763.

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Continuous observation of embryonic growth can improve understanding of the early developmental events and allow us to use parametric statistical analyses with time as a parameter. A cinematographic study such as that reported here utilizes time-lapse video recording. Previously published methods for time-lapse video recording have involved building an incubator around a microscope, a process that is both expensive and laborious. Here we present a simplified method for time-lapse video recording of early bovine embryo development. The embryos were cultured during a 24-hour period in a standard
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Straight, Aaron F., John W. Sedat, and Andrew W. Murray. "Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast." Journal of Cell Biology 143, no. 3 (1998): 687–94. http://dx.doi.org/10.1083/jcb.143.3.687.

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The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three of these motors by video microscopy of motor mutants whose microtubules and centromeres were marked with green fluorescent protein. Despite their functional overlap, each motor mutant has a specific defect in mitosis: cin8Δ mutants lack the rapid phase of anaphase B, kip1Δ mutants show defects in the slow phase of anaphase B, and kip3Δ mutants
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Yuan, Liang, and Junda Zhu. "Video Object Tracking in Neural Axons with Fluorescence Microscopy Images." Journal of Applied Mathematics 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/423876.

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Neurofilament is an important type of intercellular cargos transmitted in neural axons. Given fluorescence microscopy images, existing methods extract neurofilament movement patterns by manual tracking. In this paper, we describe two automated tracking methods for analyzing neurofilament movement based on two different techniques: constrained particle filtering and tracking-by-detection. First, we introduce the constrained particle filtering approach. In this approach, the orientation and position of a particle are constrained by the axon’s shape such that fewer particles are necessary for tra
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Phillipson, Mia, Bryan Heit, Pina Colarusso, Lixin Liu, Christie M. Ballantyne, and Paul Kubes. "Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade." Journal of Experimental Medicine 203, no. 12 (2006): 2569–75. http://dx.doi.org/10.1084/jem.20060925.

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The prevailing view is that the β2-integrins Mac-1 (αMβ2, CD11b/CD18) and LFA-1 (αLβ2, CD11a/CD18) serve similar biological functions, namely adhesion, in the leukocyte recruitment cascade. Using real-time and time-lapse intravital video-microscopy and confocal microscopy within inflamed microvessels, we systematically evaluated the function of Mac-1 and LFA-1 in the recruitment paradigm. The chemokine macrophage inflammatory protein-2 induced equivalent amounts of adhesion in wild-type and Mac-1−/− mice but very little adhesion in LFA-1−/− mice. Time-lapse video-microscopy within the postcapi
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Bell, Paul B., Margaretha Lindroth, Karin Hedberg, Bengt-Arne Fredriksson, and Åke Wasteson. "Reorganization of the Actin Cytoskeleton in Human Fibroblasts Induced by PDGF and Neomycin." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 180–81. http://dx.doi.org/10.1017/s0424820100158443.

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Among the earliest effects on cells of platelet derived growth factor (PDGF), a growth-stimulating factor normally occurring in blood, are stimulation of lamellipodia formation and induction of changes in the actin cytoskeleton. We have investigated these phenomena by time lapse video (TLV) and fluorescence light microscopy (FLM), and high resolution scanning electron microscopy (SEM) using two strains of human diploid fibroblasts: ICIG-7 from embryonic lung and AG1523 from foreskin, grown either on coverglasses or on carbon-stabilized Formvar-coated gold electron microscope grids. Cells were
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Cole, Richard W., Alexey Khodjakov, William H. Wright, and Conly L. Rieder. "A Differential Interference Contrast-Based Light Microscopic System for Laser Microsurgery and Optical Trapping of Selected Chromosomes during Mitosis In Vivo." Microscopy and Microanalysis 1, no. 5 (1995): 203–15. http://dx.doi.org/10.1017/s1431927695112039.

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Laser microsurgery and laser-generated optical force traps (optical tweezers) are both valuable light microscopic-based approaches for studying intra- and extracellular motility processes, including chromosome segregation during mitosis. Here we describe a system in use in our laboratory that allows living cells to be followed by high-resolution differential interference contrast (DIC) video-enhanced time-lapse light microscopy while selected mitotic organelles and spindle components are subjected to laser microsurgery and/or manipulation with an optical force trap. This system couples the out
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Sanderson, Christopher M., Michael Way, and Geoffrey L. Smith. "Virus-Induced Cell Motility." Journal of Virology 72, no. 2 (1998): 1235–43. http://dx.doi.org/10.1128/jvi.72.2.1235-1243.1998.

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ABSTRACT Many viruses induce profound changes in cell metabolism and function. Here we show that vaccinia virus induces two distinct forms of cell movement. Virus-induced cell migration was demonstrated by an in vitro wound healing assay in which infected cells migrated independently into the wound area while uninfected cells remained relatively static. Time-lapse microscopy showed that the maximal rate of migration occurred between 9 and 12 h postinfection. Virus-induced cell migration was inhibited by preinactivation of viral particles with trioxsalen and UV light or by the addition of cyclo
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Swanson, J. A., M. T. Johnson, K. Beningo, P. Post, M. Mooseker, and N. Araki. "A contractile activity that closes phagosomes in macrophages." Journal of Cell Science 112, no. 3 (1999): 307–16. http://dx.doi.org/10.1242/jcs.112.3.307.

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Studies of Fc-mediated phagocytosis by mouse macrophages identified a contractile activity at the distal margins of forming phagosomes. Time-lapse video microscopic analysis of macrophages containing rhodamine-labeled actin and fluorescein dextran showed that actin was concentrated at the distal margins of closing phagosomes. Phagocytosis-related contractile activities were observed when one IgG-opsonized erythrocyte was engaged by two macrophages. Both cells extended pseudopodia until they met midway around the erythrocyte. It was then constricted and pulled into two phagosomes, which remaine
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Laster, S. M., J. G. Wood, and L. R. Gooding. "Target-induced changes in macrophage migration may explain differences in lytic sensitivity among simian virus 40-transformed fibroblasts." Journal of Immunology 141, no. 1 (1988): 221–27. http://dx.doi.org/10.4049/jimmunol.141.1.221.

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Abstract Time-lapse video microscopy has been used to study macrophage movement in the presence of SV40-transformed fibroblasts. In all, five different SV40-transformed cell lines (an uncloned line and four clones derived from it) were tested for their affects on the movement of LPS-activated macrophages (AM). Conditions for video microscope recording were designed to simulate, as best as possible, the conditions used in a 51Cr-release cytotoxicity assay. Our analysis shows that these targets had a range of effects on macrophage migration, from stimulation to complete inhibition of movement. T
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Qian, X., S. K. Goderie, Q. Shen, J. H. Stern, and S. Temple. "Intrinsic programs of patterned cell lineages in isolated vertebrate CNS ventricular zone cells." Development 125, no. 16 (1998): 3143–52. http://dx.doi.org/10.1242/dev.125.16.3143.

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Using long-term, time-lapse video-microscopy, we investigated how single progenitor cells isolated from the early embryonic cerebral cortex produce neurons and glia over time. Clones of 10 cells or less were produced by short symmetric or asymmetric division patterns, commonly terminating in a ‘pair progenitor’ for two morphologically identical neurons. Larger trees were composites of these short sub-lineages: more prolific neuroblasts underwent repeated asymmetric divisions, each producing a minor neuroblast that typically made (3/4)10 progeny, and a sister cell capable of generating more pro
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Rozprawy doktorskie na temat "Two time lapse video microscopy"

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Bornemann, Lea [Verfasser], and Matthias [Akademischer Betreuer] Gunzer. "Exploring immune cell migration and its prognostic and diagnostic potential by time-lapse video and light sheet fluorescence microscopy / Lea Bornemann ; Betreuer: Matthias Gunzer." Duisburg, 2021. http://d-nb.info/1234911256/34.

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MORTATI, LEONARDO MICHAEL. "Coherent Anti-Stokes Raman Scattering, Second Harmonic Generation and Two-Photon Excitation Fluorescence Multimodal Microscope: Realization, Metrological Characterization and Applications in Regenerative Medicine." Doctoral thesis, Politecnico di Torino, 2013. http://hdl.handle.net/11583/2509905.

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In the frame of the research in biology and in particular in regenerative medicine, it is widely requested the ability to perform measurements that have a low impact on the observed biological systems. Many measurements imply sample modifications and also sample fixation avoiding living samples measurements. In this doctoral thesis it is presented the realization of an advanced optical multimodal microscope that integrates coherent anti-Stokes Raman scattering, second harmonic generation and two-photon excitation fluorescence techniques in a single powerful tool. The combination of all these m
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Betizeau, Marion. "Molecular and cellular characterization of apical and basal progenitors in the primate developing cerebral cortex." Thesis, Lyon, École normale supérieure, 2013. http://www.theses.fr/2013ENSL0845/document.

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Le cortex cérébral primate a subi des modifications majeures pendant l'évolution qui ont permis le développement de fonctions cognitives supérieures. Un accroissement massif a eu lieu avec l'extension spécifique des couches supragranulaires et une forte expansion tangentielle. Le cortex primate ne possède pas uniquement davantage de neurones, comparé au rongeur, mais aussi des différences qualitatives. Ceci suggère des différences qualitatives pendant le développement du cortex.Une zone proliférative corticale supplémentaire a été identifiée chez le singe macaque: la zone subventriculaire exte
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Części książek na temat "Two time lapse video microscopy"

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Carvalho, Lara, and Carl-Philipp Heisenberg. "Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-977-2_17.

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Wang, Yu, Farshid Moussavi, and Peter Lorenzen. "Automated Embryo Stage Classification in Time-Lapse Microscopy Video of Early Human Embryo Development." In Advanced Information Systems Engineering. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-40763-5_57.

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Paniagua-Herranz, Lucía, Rosa Gómez-Villafuertes, David de Agustín-Durán, et al. "Time-Lapse Video Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro." In Imaging and Tracking Stem Cells. Springer US, 2019. http://dx.doi.org/10.1007/7651_2019_219.

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Kavanaugh, Wade, and Stephen B. Nguyen. "473 Inches at 60 Frames Per Second." In Making the Geologic Now. punctum books, 2012. https://doi.org/10.21983/p3.0014.1.32.

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473 Inches at 60 Frames per Secondwas a site-specific sculptural installation with reference to the Wisconsin Glacial Episode, a glacial period that left Minnesota and parts of Wisconsin covered with numerous lakes and rivers. At the Soap Factory in Minneapolis, MN, Wade Kava-naugh and Stephen B. Nguyen created a two-part artwork that consisted of a glacial ice sheet made of textured white kraft paper and a time lapse video. The two works were physically and temporally downscaled to human scale. The sculpture was created in a way that the final stop-motion documentation read as if the glacier
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Grünwald, J., U. Bongartz, R. Bloom, P. Wülfroth, and C. C. Haudenschild. "The Quantitive Analysis of Cell Motility in Cultures of Smooth Muscle Cells, Endothelial Cells and Monocyte/Macrophages in Individual and Co-Culture Systems, Using Time-Lapse Video-Microscopy in Correlation with Progression of Atherosclerosis." In Atherosclerotic Plaques. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4757-0438-9_24.

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Thorogood, Peter v., and dee Amanze. "Studying cell movements in vivo by time-lapse video microscopy." In Essential Developmental Biology. Oxford University PressOxford, 1993. http://dx.doi.org/10.1093/oso/9780199634231.003.0016.

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Abstract Cell movement plays an important role during embryonic development (1). Much of what is presently known about cell movement is based on the behaviour of cultured cells. Despite refinements of culture conditions, however, extrapolation from events in vitro to behaviour in the embryo is difficult. Direct observation of living cells in vivo, when this can be done, is clearly preferable. Naturally transparent tissues such as the cornea (2), nematode embryos (3) (Chapter 2) and teleost fish embryos (4) (Chapters 4 and 9) (Figure /) lend themselves particularly well to in vivo analyses of c
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Weiss, Dieter G., Willi Maile, Robert A. Wick, and Walter Steffen. "Video microscopy." In Light Microscopy in Biology. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780199636709.003.0003.

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Abstract A new quality of microscopy, called video microscopy, emerges, if one observes the specimen, instead of with the human eye, with a video camera connected to video processing equipment working at real time. Video microscopy is, there fore, much more than just adding a camera and monitor to the microscope to share the images with a larger audience. More recently, electronic devices other than video cameras, such as high sensitivity charge coupled device (CCD) cameras and scanning light detector systems for confocal microscopy have been added to microscopes. The three fields (i) video-en
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Streszczenia konferencji na temat "Two time lapse video microscopy"

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Carter, P. W., R. V. Davis, M. A. Kamrath, and P. E. Reed. "Mechanistic Studies of Sub Stoichiometric Crystal Growth Inhibitors." In CORROSION 1995. NACE International, 1995. https://doi.org/10.5006/c1995-95474.

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Abstract An advanced understanding of the fundamental scale forming processes is critical to developing superior cooling water scale control chemistries. This paper describes the influence of interfacial structure on calcium carbonate precipitation and scale formation. Scaling kinetics are dependent upon the molecular structure of the interface formed between substoichiometric inhibitors and calcium carbonate solid states. Comparative studies of calcium carbonate inhibition activity of select 1,1-diphosphonates, 1,2-diphosphonates, and tetramethylenephosphonate inhibitors illustrate some impor
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Firdaus, Lulu, Astri Handayani, and Donny Danudirdjo. "Rat Mesenchymal Stem Cell Segmentation on Time-lapse Fluorescence Microscopy Video with Two-Dimensional Bandpass Filters." In 2023 29th International Conference on Telecommunications (ICT). IEEE, 2023. http://dx.doi.org/10.1109/ict60153.2023.10374039.

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He, Weijun, Xiaoxu Wang, Dimitris Metaxas, Robin Mathew, and Eileen White. "Cell segmentation for division rate estimation in computerized video time-lapse microscopy." In Biomedical Optics (BiOS) 2007, edited by Fred S. Azar. SPIE, 2007. http://dx.doi.org/10.1117/12.717590.

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Masuda, Michiaka, and Keigi Fujiwara. "Three Distinct Types of Morphological Responses of Cultured Vascular Endothelial Cells to Physiological Levels of Fluid Shear Stress." In ASME 2003 1st International Conference on Microchannels and Minichannels. ASMEDC, 2003. http://dx.doi.org/10.1115/icmm2003-1124.

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Vascular endothelial cells are known to respond to fluid shear stress. To gain insights into the mechanism of flow response by these cells, various types of in vitro devices in which endothelial cells can be cultured under flowing culture medium have been designed. Using such a device, one can apply known levels of (usually laminar) fluid shear stress to cultured endothelial cells. We have made two types of devices: a viscometer-based cone-and-plate flow apparatus and a parallel plate chamber. The cone-and-plate apparatus is used to do biochemical analyses of flow effects on cells while the pa
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Nelson, Chas. "Open source optical gating technologies enable two-day phase-locked time-lapse 3D fluorescence imaging of the developing and beating zebrafish heart." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.967.

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Price, B., Y. Li, S. Liu, and A. Abedini. "Microfluidics – A New Tool to Differentiate Chemistry for Fracs – Functional Frac Performance Vs Reservoir Performance." In ADIPEC. SPE, 2024. http://dx.doi.org/10.2118/222677-ms.

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Abstract Unconventional reservoirs play an important role in the global energy supply nowadays due to recent advancements in hydraulic fracturing. It has been reported that the selection of completion chemicals have a significant impact on oil and gas production due to fluid incompatibility and polymer-induced formation damage. To optimize oil and gas production, flowback efficiency, and fracturing fluid-induced permeability damage, different completion fluid packages were evaluated using microfluidics. Two microfluidics chips were designed to evaluate regain conductivity and flowback efficien
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Liu, S., Y. Li, and A. Abedini. "Microfluidics – A New Tool to Differentiate Chemistry for Fracs – Functional Frac Performance Vs Reservoir Performance." In SPE International Conference on Oilfield Chemistry. SPE, 2025. https://doi.org/10.2118/224229-ms.

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Abstract Unconventional reservoirs play an important role in the global energy supply nowadays due to recent advancements in hydraulic fracturing. It has been reported that the selection of completion chemicals has a significant impact on oil and gas production due to fluid incompatibility and polymer-induced formation damage. To optimize oil and gas production, flowback efficiency, and fracturing fluid-induced permeability damage, different completion fluid packages were evaluated using microfluidics. Two microfluidics chips were designed to evaluate regain conductivity and flowback efficienc
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Vourc’h, Thomas, Julien Léopoldès, Annick Méjean, and Hassan Peerhossaini. "Motion of Active Fluids: Diffusion Dynamics of Cyanobacteria." In ASME 2016 Fluids Engineering Division Summer Meeting collocated with the ASME 2016 Heat Transfer Summer Conference and the ASME 2016 14th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/fedsm2016-7526.

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Cyanobacteria are photosynthetic micro-organisms colonizing all aquatic and terrestrial environments. The motility of such living micro-organisms should make their diffusion distinct from typical Brownian motion. This diffusion can be investigated in terms of global behavior (Fickian or not) and in terms of displacement probabilities, which provide more detail about the motility process. Using cyanobacterium Synechocystis sp. PCC 6803 as the model micro-organism, we carry out time-lapse video microscopy to track and analyze the bacteria’s trajectories, from which we compute the mean-squared di
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Wu, Jing, Mohammad S. Alam, KM Rafidh Hassan, Jeffrey C. Suhling, and Pradeep Lall. "Investigation and Comparison of Aging Effects in SAC305 and Doped SAC+X Solders Exposed to Isothermal Aging." In ASME 2020 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/ipack2020-2695.

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Abstract Microstructural evolution occurs in lead free Sn-Ag-Cu (SAC) solder joints exposed to isothermal aging. Such changes lead to degradations in the mechanical properties and creep behavior of the solder, and can result in dramatic reductions in the board level reliability of lead-free electronic assemblies subjected to aging. In our recent research, Scanning Electron Microscopy (SEM) has been used to: (1) monitor aging induced microstructural changes occurring within fixed regions in selected lead-free solder joints, (2) create time-lapse imagery of the microstructure evolution, and (3)
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Greer, Julia R., Ju-Young Kim, and Steffen Brinckmann. "In-Situ Investigation of Plasticity at Nano-Scale." In ASME 2008 9th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2008. http://dx.doi.org/10.1115/esda2008-59117.

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Mechanical behavior of crystals is dictated by dislocation motion in response to applied force. While it is extremely difficult to directly observe the motion of individual dislocations, several correlations can be made between the microscopic stress-strain behavior and dislocation activity. Here, we present for the first time the differences observed between mechanical behavior in two fundamental types of crystals: face-centered cubic, fcc (Au, Cu, Al, Ni, etc.) and body-centered cubic, bcc (W, Cr, Mo, Nb, etc.) with sub-micron dimensions subjected to in-situ micro-compression in SEM chamber.
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