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Artykuły w czasopismach na temat „Umbilical Vein PH”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Kang, Hyun, Tae-Yun Sung, Young Seok Jee, et al. "A Comparison of Norepinephrine versus Phenylephrine to Prevent Hypotension after Spinal Anesthesia for Cesarean Section: Systematic Review and Meta-Analysis." Journal of Personalized Medicine 14, no. 8 (2024): 803. http://dx.doi.org/10.3390/jpm14080803.

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Background: This systematic review and meta-analysis aimed to compare the effects of using phenylephrine or norepinephrine on the pH and base excess (BE) of the umbilical artery and vein in parturients undergoing cesarean section. Methods: The study protocol was registered in INPLASY. Independent researchers searched Ovid-Medline, Ovid-EMBASE, and Cochrane Central Register of Controlled Trials (CENTRAL) databases and Google Scholar for relevant randomized controlled trials (RCTs). The primary outcome of this study was the umbilical artery (UA) or umbilical vein (UV) pH as neonatal condition at

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2

Park, Jong Cook, Kwan Sik Park, and Sung Yob Kim. "Effects of Left Uterine Displacement on General Anesthesia for Cesarean Section." Journal of Medicine and Life Science 2, no. 1 (2004): 41–48. http://dx.doi.org/10.22730/jmls.2004.2.1.41.

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Background: The hazards of aorto-caval compression by the gravid uterus in late pregnancy are well documented.The provision of left uterine displacement (LUD) has proven highly beneficial to both mother and infant. We decided to investigate the effect of a new wedge to provide left uterine displacement.Methods: 40 full-termed patients undergoing elective Caesarean section under a standardized regimen of general anesthesia were studied. We randomly divided the patients into the control group and the LUD group and assessed changes of maternal MAP and HR and infant's Apgar scores and cord blood g

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3

Wilkening, R. B., and G. Meschia. "Effect of occluding one umbilical artery on placental oxygen transport." American Journal of Physiology-Heart and Circulatory Physiology 260, no. 4 (1991): H1319—H1325. http://dx.doi.org/10.1152/ajpheart.1991.260.4.h1319.

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Placental O2 transport was studied in seven fetal lambs before and after occluding one of the two umbilical arteries. Ethanol was used to measure uterine and umbilical blood flows using the steady-state transplacental diffusion method. Blood samples were drawn from umbilical artery, umbilical vein, both uterine veins, and maternal artery and analyzed for blood flow indicator, O2 content, PO2, PCO2, and pH. Occlusion reduced the placental mass and the uterine blood flow, which was available for transplacental exchange, to 49.5 and 46.5% of control, respectively. After occlusion, fetal blood pre

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4

Olsen, Randi, Paul Webster, and John Bjarne Hansen. "Subcellular Localozation of TFPI in Human Umbilical Vein Endothelial Cells (HUVEC)." Proceedings, annual meeting, Electron Microscopy Society of America 54 (August 11, 1996): 44–45. http://dx.doi.org/10.1017/s0424820100162685.

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Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor which regulates tissue factor (TF) induced blood coagulation by inhibiting the action of factor VIIa-TF complexes and factor Xa. In plasma, more than 80 % of the TFPI is bound to lipoproteins, 10 -20 % is carrier free, whereas platelets contains a small amount of TFPI. A major pool is probably assosiated with the vascular endothelium (1). This pool may have a crucial regulatory function because the endothelial surface serves as a binding site for factor VII/VIIa, mediating the activation of the extrinsic coagulation system.T

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5

Czeresnia, Jonathan Mamber, Edward Araujo Júnior, Eduardo Cordioli, Wellington P. Martins, Luciano Marcondes Machado Nardozza, and Antonio Fernandes Moron. "Applicability of the Rapid Biophysical Profile in Antepartum Fetal Well-Being Assessment in High-Risk Pregnancies from a University Hospital in São Paulo, Brazil: Preliminary Results." ISRN Obstetrics and Gynecology 2013 (June 27, 2013): 1–4. http://dx.doi.org/10.1155/2013/329542.

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Objective. To evaluate the clinical applicability of the rapid biophysical profile (rBPP), comparing results of the rBPP to umbilical cord pH values and Apgar scores. Methods. A cross-sectional study was conducted with 37 pregnant women from our high-risk service. All of them gave birth at our institution. rBPP was conducted up to 24 h prior to delivery and pH values were obtained from the umbilical vein immediately after birth. The mean and standard deviations for maternal age, gestational age at birth, pH values, and Apgar score in the 1st and 5th minutes after birth were calculated. An unpa

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6

Samchuk, P. M., I. Kh Tsaroeva, A. I. Ishchenko, and E. L. Azoeva. "Assessment of electrolyte balance and oximetry of umbilical cord blood in newborns with fetal growth restriction." Voprosy ginekologii, akušerstva i perinatologii 21, no. 2 (2022): 49–54. http://dx.doi.org/10.20953/1726-1678-2022-2-49-54.

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Objective. To study the characteristics of oximetry and electrolyte balance of umbilical cord arterial and venous blood in newborns with fetal growth restriction. Patients and methods. Laboratory analysis of oximetry and electrolyte balance in blood samples from the umbilical artery and vein in 40 newborns after vaginal delivery at term and uncomplicated pregnancy (the control group) and in 30 newborns with fetal growth restriction after vaginal delivery at term (the study group) was performed. Results. The analysis revealed that the levels of FHbF, pO2, sO2, FO2Hb, FCOHb, cCa2+ and ctO2 in um

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7

Alarcón-Rodríguez, Raquel, María Paz Martín-Álvarez, Jessica García-González, et al. "Determination of Foetal Scalp Blood Sampling pH as an Indicator of Loss of Foetal Well-Being in Women Undergoing Caesarean Section." Healthcare 11, no. 5 (2023): 725. http://dx.doi.org/10.3390/healthcare11050725.

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Asphyxia during birth is one of the three leading causes of neonatal morbidity and mortality among newborns carried to term. The objective of this study was to evaluate the measurement of the foetal scalp blood pH as a measure of foetal status, evaluating: cord gases, meconium-stained fluid, APGAR score or the need for neonatal resuscitation in pregnant women undergoing caesarean sections. A cross-sectional study was carried out over a period of 5 years (2017–2021) at the Hospital de Poniente (southern Spain). A total of 127 pregnant women participated from whom a foetal scalp blood pH sample

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8

Polyanin, A. A., and I. J. Kogan. "Role of atrial natriuretic peptide (ANP) in feto-placental and maternal circulation." Journal of obstetrics and women's diseases 51, no. 1 (2002): 25–32. http://dx.doi.org/10.17816/jowd89948.

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ANPwas measured in maternal cubital vein (M), umbilical vein (UV) and artery (UA) in 39 women at term delivery (23 vaginal, 16 caesarean): 15 - normal pregnancy; 24 pregnancies were complicated by placental insufficiency, 14 - by preeclampsia, 10 - by diabetes mellitus. Blood gases and pH in UA, UV were measured. M, UA, UV ANPat caesarean section was similar to that in vaginal delivery (p0,05). In normal and complicated pregnancies, UA ANPwas significantly higher than ANPin M and UV. In pregnancies complicated by diabetes mellitus, UV ANPwas higher than ANPin UA (21, 93 fmol/l and 13, 03

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9

Rizzo, Giuseppe, Cecilia Mattioli, Ilenia Mappa, et al. "Hemodynamic factors associated with fetal cardiac remodeling in late fetal growth restriction: a prospective study." Journal of Perinatal Medicine 47, no. 7 (2019): 683–88. http://dx.doi.org/10.1515/jpm-2019-0217.

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Abstract Background Altered cardiac geometry affects a proportion of fetuses with growth restriction (FGR). The aim of this study was to explore the hemodynamic factors associated with cardiac remodeling in late FGR. Methods This was a prospective study of singleton pregnancies complicated by late-onset FGR undergoing assessment of left (LV) and right (RV) ventricular sphericity-index (SI). The study population was divided in two groups according to the presence of cardiac remodelling, defined as LVSI <5th centile. The following outcomes were explored: gestational age at birth, birthweight,

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10

Ikebuchi, Motoyoshi, Atsunori Kashiwagi, Takayuki Asahina, et al. "Effect of medium pH on glutathione redox cycle in cultured human umbilical vein endothelial cells." Metabolism 42, no. 9 (1993): 1121–26. http://dx.doi.org/10.1016/0026-0495(93)90269-t.

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11

Fuentes, Gonzalo, Paola Valero, Macelo Cornejo, et al. "The intracellular pH in human umbilical vein endothelial cells from gestational diabesity is unaltered by insulin.*." Placenta 83 (August 2019): e101. http://dx.doi.org/10.1016/j.placenta.2019.06.320.

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12

Godik, O. S., D. S. Diehtiarova, and O. I. Kvilinska. "Extrahepatic portal vein obstruction in children of the first year of life: diagnostic and treatment approaches." UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS, no. 1(97) (March 28, 2024): 72–77. http://dx.doi.org/10.15574/pp.2024.97.72.

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Extrahepatic portal vein obstruction (EHPVO) is an important cause of symptomatic portal hypertension (PH) in pediatric population. PH may lead to serious complications including variceal bleeding and hypersplenism. Literature data on infants under 12 months old with EHPVO is poor. Aim - to analyze our experience in treatment of children with symptomatic PH in children of the first year of life, the peculiarities of the course of EHPVO in them, and acknowledge readers with diagnostic algorithm of patients with EHPVO that ensures the timely start of treatment and reduce the risk of life-threate

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13

Hansen, John-Bjarne, Randi Olsen, and Paul Webster. "Association of Tissue Factor Pathway Inhibitor With Human Umbilical Vein Endothelial Cells." Blood 90, no. 9 (1997): 3568–78. http://dx.doi.org/10.1182/blood.v90.9.3568.

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AbstractTissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation system, synthesized in endothelial cells, which has recently been shown to play an important role in the regulation of activated coagulation factors at the endothelial cell surface. In the present study we investigated the subcellular localization and metabolism of TFPI in human umbilical vein endothelial cells (HUVEC). Immunocytochemical labeling of HUVEC with anti-TFPI showed specific labeling associated with the cell surface and with many intracellular organelles including the Golgi co

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14

Tsiros, Divyabharathy, Casey E. Sheehy, and Matthew A. Nugent. "Heparin–Avastin Complexes Show Enhanced VEGF Binding and Inhibition of VEGF-Mediated Cell Migration." International Journal of Translational Medicine 1, no. 2 (2021): 101–15. http://dx.doi.org/10.3390/ijtm1020008.

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Bevacizumab (known by the tradename Avastin) is an antibody that binds VEGF and blocks its binding to VEGF receptors on endothelial cells, and is used to treat cancers and other diseases associated with excessive vascular growth. Our previous findings showed enhanced VEGF binding to Avastin in the presence of heparin, indicating that colocalizing heparin with Avastin could enhance VEGF inhibitory activity. Thus, the aim of the present study was to determine if conjugating Avastin and heparin to one another would lead to enhanced anti-VEGF activity. Avastin was conjugated to either biotin or st

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15

Elvitigala, Kelum Chamara Manoj Lakmal, Wildan Mubarok, and Shinji Sakai. "Hydrogels with Ultrasound-Treated Hyaluronic Acid Regulate CD44-Mediated Angiogenic Potential of Human Vascular Endothelial Cells In Vitro." Biomolecules 14, no. 5 (2024): 604. http://dx.doi.org/10.3390/biom14050604.

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The development of hydrogels that allow vascular endothelial cells to form capillary-like networks is critical for advancing tissue engineering and drug discovery. In this study, we developed hydrogels composed of phenolated hyaluronic acid (HA-Ph) with an average molecular weight of 490–159 kDa via sonication in an aqueous solution. These hydrogels were synthesized by the horseradish peroxidase-catalyzed crosslinking of phenol moieties in the presence of hydrogen peroxide and phenolated gelatin. The sonication-degraded HA-Ph (198 kDa) significantly enhanced the migration ability of human umbi

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16

He, Bin, Binxia Shao, Cheng Cheng, et al. "miR-21-Mediated Endothelial Senescence and Dysfunction Are Involved in Cigarette Smoke-Induced Pulmonary Hypertension through Activation of PI3K/AKT/mTOR Signaling." Toxics 12, no. 6 (2024): 396. http://dx.doi.org/10.3390/toxics12060396.

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Smoking is a pathogenic factor for pulmonary hypertension (PH). Our previous study showed that serum miR-21 levels are elevated in smokers. miR-21 is considered as engaged in the PH process; however, its mechanisms remain unclear. In this investigation, we found that in the lung tissue of smoking-induced PH patients, the levels of miR-21 and aging markers (p21 and p16) were upregulated, and the function of pulmonary vascular endothelial cells was also impaired. Exposure of mice to cigarette smoke (CS) for four months caused similar changes in lung tissues and increased pulmonary arterial press

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17

Zhang, Zhen, Yi Liu, Xuelian Tao, et al. "Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels." Polymers 15, no. 8 (2023): 1925. http://dx.doi.org/10.3390/polym15081925.

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Cell cultures of dispersed cells within hydrogels depict the interaction of the cell–extracellular matrix (ECM) in 3D, while the coculture of different cells within spheroids combines both the effects of cell–cell and cell–ECM interactions. In this study, the cell co-spheroids of human bone mesenchymal stem cells/human umbilical vein endothelial cells (HBMSC/HUVECs) are prepared with the assistance of a nanopattern, named colloidal self-assembled patterns (cSAPs), which is superior to low-adhesion surfaces. A phenol-modified gelatin/hyaluronan (Gel-Ph/HA-Ph) hydrogel is used to encapsulate the

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18

Araos, J., A. Saavedra, C. Sanhueza, A. Leiva, M. A. Ramírez, and L. Sobrevia. "Sodium/proton exchanger subtype 1 regulates intracellular pH in human umbilical vein endothelial cells from gestational diabetes mellitus." Placenta 51 (March 2017): 129–30. http://dx.doi.org/10.1016/j.placenta.2017.01.107.

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Kajani, Abolghasem Abbasi, and Masoud Ayatollahi Mehrgardi. "Fluorescence resonance energy transfer monitoring of pH-responsive doxorubicin release from carbon dots/aptamer functionalized magnetic mesoporous silica." Nanomedicine 16, no. 8 (2021): 627–39. http://dx.doi.org/10.2217/nnm-2020-0410.

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Aim: To develop a novel theranostic nanoplatform for simultaneous fluorescent monitoring and stimuli-triggered drug delivery. Materials & methods: Different microscopic and spectroscopic techniques were used for the characterization of nanocarriers. MCF-7 and human umbilical vein endothelial cell lines were cultured and treated with different doses of doxorubicin-loaded nanocarriers. The cell viability and drug release were studied using MTT assay and fluorescence microscopy. Results: Biocompatible and mono-disperse nanocarriers represent hollow and mesoporous structures with the calculate

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20

Kurz, D. J., S. Decary, Y. Hong, and J. D. Erusalimsky. "Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells." Journal of Cell Science 113, no. 20 (2000): 3613–22. http://dx.doi.org/10.1242/jcs.113.20.3613.

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Senescence-associated (beta)-galactosidase is widely used as a biomarker of replicative senescence. However, it remains unknown whether this is a distinct enzyme active at pH 6, and differentially expressed in senescence, or a manifestation of an increase in the classic acid lysosomal (beta)-galactosidase. Here we have investigated the origin of senescence-associated-(beta)-galactosidase activity by modifying the intracellular and lysosomal pH of young and senescent human umbilical vein endothelial cells and examining the effect of these manipulations on the levels of activity, using a flow cy

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21

Kuriyama, Sachiko, Yoshiteru Morio, Michie Toba, et al. "Genistein attenuates hypoxic pulmonary hypertension via enhanced nitric oxide signaling and the erythropoietin system." American Journal of Physiology-Lung Cellular and Molecular Physiology 306, no. 11 (2014): L996—L1005. http://dx.doi.org/10.1152/ajplung.00276.2013.

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Upregulation of the erythropoietin (EPO)/EPO receptor (EPOR) system plays a protective role against chronic hypoxia-induced pulmonary hypertension (hypoxic PH) through enhancement of endothelial nitric oxide (NO)-mediated signaling. Genistein (Gen), a phytoestrogen, is considered to ameliorate NO-mediated signaling. We hypothesized that Gen attenuates and prevents hypoxic PH. In vivo, Sprague-Dawley rats raised in a hypobaric chamber were treated with Gen (60 mkg/kg) for 21 days. Pulmonary hemodynamics and vascular remodeling were ameliorated in Gen-treated hypoxic PH rats. Gen also restored c

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22

Bandyopadhyay, Anjishnujit, Chhavi Sawhney, Partha Haldar, and Sharmishtha Pathak. "Effect of prophylactic phenylephrine versus norepinephrine on foeto-maternal outcomes in caesarean delivery under neuraxial anaesthesia: A systematic review and meta-analysis with trial sequential analysis." Indian Journal of Anaesthesia 69, no. 7 (2025): 638–49. https://doi.org/10.4103/ija.ija_1063_24.

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Background and Aims: The prevention of hypotension in parturients scheduled for caesarean section is of utmost importance for improving foeto-maternal outcomes. We compared the prophylactic use of phenylephrine (PE) and norepinephrine (NE) on foeto-maternal outcomes in women undergoing caesarean delivery under neuraxial anaesthesia. Umbilical artery (UA) pH was the primary objective, and umbilical vein (UV) pH, APGAR scores, incidence of bradycardia, hypotension, and hypertension were secondary objectives. Methods: PubMed, EMBASE, Web of Science, Google Scholar, and CENTRAL databases were sear

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23

Crichton, C. A., A. G. B. Templeton, and G. L. Smith. "Effect of altered bathing pH on calcium activated force in toxin permeabilised rat portal vein and human umbilical artery." Cardiovascular Research 28, no. 9 (1994): 1378–84. http://dx.doi.org/10.1093/cvr/28.9.1378.

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Alonazi, Mona, Najeh Krayem, Mona G. Alharbi, et al. "Functional Characterization and Anti-Tumor Effect of a Novel Group II Secreted Phospholipase A2 from Snake Venom of Saudi Cerastes cerates gasperetti." Molecules 28, no. 18 (2023): 6517. http://dx.doi.org/10.3390/molecules28186517.

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Secreted phospholipases A2 are snake-venom proteins with many biological activities, notably anti-tumor activity. Phospholipases from the same snake type but different geographical locations have shown similar biochemical and biological activities with minor differences in protein sequences. Thus, the discovery of a new phospholipase A2 with unique characteristics identified in a previously studied venom could suggest the origins of these differences. Here, a new Group II secreted phospholipase A2 (Cc-PLA2-II) from the snake venom of Saudi Cerastes cerastes gasperetti was isolated and characte

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25

Wagner, D. D., T. Mayadas, and V. J. Marder. "Initial glycosylation and acidic pH in the Golgi apparatus are required for multimerization of von Willebrand factor." Journal of Cell Biology 102, no. 4 (1986): 1320–24. http://dx.doi.org/10.1083/jcb.102.4.1320.

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Two conditions were identified that interfered with the complex polymerization process in biosynthesis of von Willebrand factor (vWf). Treatment of human umbilical vein endothelial cells with tunicamycin inhibited N-linked glycosylation of nascent vWf and the resulting pro-vWf monomers failed to dimerize. The single subunits accumulated in the endoplasmic reticulum and were neither processed further nor secreted. In the presence of a weak base (ammonium chloride or chloroquine), interdimer disulfide bond formation was inhibited in a dose-dependent manner. This process appeared therefore to be

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26

Verhaeghe, J., J. Billen, and LC Giudice. "Insulin-like growth factor-binding protein-1 in umbilical artery and vein of term fetuses with signs suggestive of distress during labor." Journal of Endocrinology 170, no. 3 (2001): 585–90. http://dx.doi.org/10.1677/joe.0.1700585.

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Insulin-like growth factor-binding protein-1 (IGFBP-1) is believed to be an inhibitory factor for fetal growth. The regulation of IGFBP-1 secretion in the fetus is uncertain, although insulin and oxygen tension (PO2) and saturation are thought to play a role. We studied IGFBP-1 levels in umbilical cord artery (UA) and vein (UV) of 98 singleton fetuses at term with clinical signs of distress during labor, i.e. meconium-stained liquor or/and an abnormal fetal heart rate tracing. Blood gas values and serum C-peptide and IGFBP-1 concentrations were measured in both UA and UV. Twenty-five fetuses h

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27

Drouet, Maryline, Feng Chai, Christine Barthélémy, et al. "Influence of Vancomycin Infusion Methods on Endothelial Cell Toxicity." Antimicrobial Agents and Chemotherapy 59, no. 2 (2014): 930–34. http://dx.doi.org/10.1128/aac.03694-14.

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ABSTRACTPeripheral intravenous therapy is frequently used in routine hospital practice and, due to various factors, its most common side effect is phlebitis. The infusion of vancomycin is particularly associated with phlebitis despite its widespread use. French guidelines recommend central intravenous infusion for high concentrations of vancomycin, but peripheral intravenous therapy is often preferred in intensive care units. Methods of vancomycin infusion are either intermittent infusion or continuous infusion. A comparison of these methods underin vitroconditions simulating clinical use coul

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Shalev, E., Y. Zalel, and E. Weiner. "A comparison of the nonstress test, oxytocin challenge test, Doppler velocimetry and biophysical profile in predicting umbilical vein pH in growth-retarded fetuses." International Journal of Gynecology & Obstetrics 43, no. 1 (1993): 15–19. http://dx.doi.org/10.1016/0020-7292(93)90268-2.

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Patschan, Susann, Jun Chen, Olga Gealekman, et al. "Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells." American Journal of Physiology-Renal Physiology 294, no. 1 (2008): F100—F109. http://dx.doi.org/10.1152/ajprenal.00261.2007.

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Endothelial cells subjected to glycated collagen I develop premature senescence within 3–5 days, as revealed by increased senescence-associated β-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient and lysosomal permeabilization, detectable after 30 min, and preceded by the increased production of reactive oxygen species. Measurement of mitochondrial membrane potential after application of glycated collagen demonstrated that

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Escobales, N., E. Longo, E. J. Cragoe, N. R. Danthuluri, and T. A. Brock. "Osmotic activation of Na(+)-H+ exchange in human endothelial cells." American Journal of Physiology-Cell Physiology 259, no. 4 (1990): C640—C646. http://dx.doi.org/10.1152/ajpcell.1990.259.4.c640.

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Regulation of intracellular pH (pHi) via a Na(+)-H+ exchange-dependent mechanism was studied in cultured human umbilical vein endothelial cells (HEC) using the pH-sensitive fluorescent dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein, as well as measuring 22Na influx. Basal pHi of HEC incubated in a bicarbonate-free Na+ medium was 6.99 +/- 0.03. In HEC that had been acid-loaded using nigericin or a NH4Cl prepulse, pHi recovery occurred via a Na(+)-dependent mechanism that was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). The potency of amiloride derivatives to inhibit 22Na influx was

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Ghi, Tullio, Edwin Chandraharan, Stefania Fieni, et al. "Correlation between umbilical vein-to-artery delta pH and type of intrapartum hypoxia in a cohort of acidemic neonates: A retrospective analysis of CTG findings." European Journal of Obstetrics & Gynecology and Reproductive Biology 231 (December 2018): 25–29. http://dx.doi.org/10.1016/j.ejogrb.2018.10.005.

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Sanjoh, Mai, Shigehiro Hiki, Yan Lee, et al. "pDNA/poly(L-lysine) Polyplexes Functionalized with a pH-Sensitive Charge-Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells." Macromolecular Rapid Communications 31, no. 13 (2010): 1181–86. http://dx.doi.org/10.1002/marc.201000056.

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Zhou, Wei, Bin Lan, Qiong Liu, and Zhaolong Li. "Effect of Polyamidoamine-Dendrimer Nanoparticles Combined with Human Umbilical Vein Endothelial Cell Microenvironment on the Growth and Immune Infiltration of Nasopharyngeal Carcinoma Cell Line CNE-1." Science of Advanced Materials 15, no. 9 (2023): 1224–33. http://dx.doi.org/10.1166/sam.2023.4522.

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In tumor immunotherapy, Treg cells are immune suppressor cells that are difficult to completely eliminate. To address this issue, a nano-delivery system needs to be designed that includes chemotherapeutic agents and multidrug resistance reversal agents to achieve a cascade of immune chemotherapy enhancement. In this study, polyamidoamine-dendrimer (PAMAM) was utilized as the raw material to prepare a dual-sensitive nano-carrier that is both pH and reduction responsive, and loaded with the chemotherapeutic drug doxorubicin (DOX) and the multidrug resistance reversal agent, elacridar (ELC), to g

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Gavrilovskaya, Irina N., Eric J. Brown, Mark H. Ginsberg та Erich R. Mackow. "Cellular Entry of Hantaviruses Which Cause Hemorrhagic Fever with Renal Syndrome Is Mediated by β3 Integrins". Journal of Virology 73, № 5 (1999): 3951–59. http://dx.doi.org/10.1128/jvi.73.5.3951-3959.1999.

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ABSTRACT Hantaviruses replicate primarily in the vascular endothelium and cause two human diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). In this report, we demonstrate that the cellular entry of HFRS-associated hantaviruses is facilitated by specific integrins expressed on platelets, endothelial cells, and macrophages. Infection of human umbilical vein endothelial cells and Vero E6 cells by the HFRS-causing hantaviruses Hantaan (HTN), Seoul (SEO), and Puumala (PUU) is inhibited by antibodies to αvβ3 integrins and by the integrin ligand vitronect

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Xia, Lunguo, Wudi Ma, Yuning Zhou, et al. "Stimulatory Effects of Boron Containing Bioactive Glass on Osteogenesis and Angiogenesis of Polycaprolactone: In Vitro Study." BioMed Research International 2019 (March 18, 2019): 1–12. http://dx.doi.org/10.1155/2019/8961409.

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Polycaprolactone (PCL) has attracted great attention for bone regeneration attributed to its cost-efficiency, high toughness, and good processability. However, the relatively low elastic modulus, hydrophobic nature, and insufficient bioactivity of pure PCL limited its wider application for bone regeneration. In the present study, the effects of the addition of boron containing bioactive glass (B-BG) materials on the mechanical properties and biological performance of PCL polymer were investigated with different B-BG contents (0, 10, 20, 30, and 40 wt.%), in order to evaluate the potential appl

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Sun, Bing, Richard D. Vaughan-Jones, and Jun-Ichi Kambayashi. "Two distinct HCO 3 − -dependent H+ efflux pathways in human vascular endothelial cells." American Journal of Physiology-Heart and Circulatory Physiology 277, no. 1 (1999): H28—H32. http://dx.doi.org/10.1152/ajpheart.1999.277.1.h28.

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Intracellular pH (pHi) regulation in human umbilical vein endothelial cells (HUVEC) was investigated. The pHi was recorded using seminaphthorhodafluor-1 (SNARF-1). Cells were intracellularly acid loaded with NH4Cl prepulse. In HEPES-buffered Tyrode (nominally [Formula: see text]free), pHi recovery from acid load was inhibited by 1.5 mM amiloride or Na+-free solution. Additionally, in [Formula: see text]-buffered Tyrode, a[Formula: see text]-dependent pHi recovery from acidosis was evident in the presence of 1.5 mM amiloride, which mediated complete recovery of pHi (7.26). In Na+-free solution,

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Wang, Lin, Chunyan Li, Yingxin Chen, Shujun Dong, Xuesi Chen, and Yanmin Zhou. "Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells." BioMed Research International 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/530712.

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Adrenomedullin (ADM) is a bioactive regulatory peptide that affects migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells. This study investigated the effects of sustained release of ADM on the modulation activity of osteoblasts and vascular endothelial cellsin vitro. Chitosan microspheres (CMs) were developed for ADM delivery. Poly(lactic-co-glycolic) acid and nano-hydroxyapatite were used to prepare scaffolds containing microspheres with ADM. The CMs showed rough surface morphology and high porosity, and they were well-

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Guan, Xiaojun, Jose Juarez, Xiaoping Qi, et al. "Histidine-Proline Rich Glycoprotein (HPRG) binds and transduces anti-angiogenic signals through cell surface tropomyosin on endothelial cells." Thrombosis and Haemostasis 92, no. 08 (2004): 403–12. http://dx.doi.org/10.1160/th04-02-0073.

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SummaryThe anti-angiogenic properties of the histidine-proline–rich (H/P) domain of HPRG have recently been described (Juarez JC, et al. Cancer Research 2002; 62: 5344-50). However, the binding site that mediates these properties is unknown. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin (Zhang J-C, et al. Proc Natl Acad Sci USA 2002; 99: 12224-9). In this study, we demonstrate that HPRG binds wi

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Nold-Petry, Claudia, Doris Fischer, Bernd Richter, et al. "Activated protein C downregulates p38 mitogen-activated protein kinase and improves clinical parameters in an in-vivo model of septic shock." Thrombosis and Haemostasis 98, no. 11 (2007): 1118–26. http://dx.doi.org/10.1160/th07-01-0052.

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SummaryDespite the success of the anti-coagulant protease protein C (PC) in treating septic shock in humans, the signaling pathways used are still unclear. To explore the effects of treatment with PC zymogen and its activated form aPC in a setting of sepsis, we employed a piglet model of endotoxic shock. In the aPC group, we observed a 65%-90% reduction in plasmaTNF-alpha levels and a concomitant clinical improvement. Unexpectedly, administration of aPC also resulted in stabilization of the plasma pH above 7.2. Moreover, phosphorylated p38 mitogen-activated protein kinase (p38MAPK) was virtual

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Zheng, Jiawen, Xiaoxiao Tian, Baogui Xu, Falei Yuan, Jianfang Gong, and Zuisu Yang. "Collagen Peptides from Swim Bladders of Giant Croaker (Nibea japonica) and Their Protective Effects against H2O2-Induced Oxidative Damage toward Human Umbilical Vein Endothelial Cells." Marine Drugs 18, no. 8 (2020): 430. http://dx.doi.org/10.3390/md18080430.

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Five different proteases were used to hydrolyze the swim bladders of Nibea japonica and the hydrolysate treated by neutrase (collagen peptide named SNNHs) showed the highest DPPH radical scavenging activity. The extraction process of SNNHs was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 47.2 °C, a pH of 7.3 and an enzyme concentration of 1100 U/g, which resulted in the maximum DPPH clearance rate of 95.44%. Peptides with a Mw of less than 1 kDa (SNNH-1) were obtained by ultrafiltration, and exhibited good scavenging activity for hydro

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Yang, Q. R., R. M. Smets, A. Neetens, and D. Vanden Berghe. "Human retinal pigment epithelial cells from different donors continuously produce a vascular endothelial cell-stimulating factor into serum-free medium." Journal of Cell Science 104, no. 1 (1993): 211–18. http://dx.doi.org/10.1242/jcs.104.1.211.

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Mitogenic activities of human retinal pigment epithelial cell-conditioned medium (HRPE-CM) with different effects, such as inhibition, stimulation or no effect, on the proliferation of vascular endothelial cells (EC) in vitro have been reported. In this study, 14 HRPE cell lines were established from normal human eyes. Human umbilical vein endothelial cells (HUVEC) in the early passages were used as target cells to detect the mitogenic activity of HRPE-CM on the growth of vascular EC. Our results confirm that HRPE cells in culture continuously synthesize and secrete HUVEC growth substance(s) i

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Reyes-Sotelo, Brenda, Daniel Mota-Rojas, Patricia Mora-Medina, et al. "Blood Biomarker Profile Alterations in Newborn Canines: Effect of the Mother′s Weight." Animals 11, no. 8 (2021): 2307. http://dx.doi.org/10.3390/ani11082307.

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This study aims to determine the effect of the weight of bitches on liveborn and stillbirth puppies from eutocic births, and physiological blood alterations during the first minute postpartum. A total of 52 female dogs were evaluated and distributed in four categories: C1 (4.0–8.0 kg, n = 19), C2 (8.1–16.0 kg, n = 16), C3 (16.1–32.0 kg, n = 11), and C4 (32.1–35.8 kg, n = 6). The dams produced 225 liveborn puppies and 47 were classified as stillbirth type II. Blood samples were taken from the umbilical vein to evaluate the concentration of gases, glucose, lactate, calcium, hematocrit levels, an

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Leavesley, D. I., M. A. Schwartz, M. Rosenfeld, and D. A. Cheresh. "Integrin beta 1- and beta 3-mediated endothelial cell migration is triggered through distinct signaling mechanisms." Journal of Cell Biology 121, no. 1 (1993): 163–70. http://dx.doi.org/10.1083/jcb.121.1.163.

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Human umbilical vein endothelial cell attachment, spreading and migration on collagen and vitronectin are mediated by integrins alpha 2 beta 1 and alpha v beta 3, respectively, and these events take place in the absence of cytokines, growth factors, or chemoattractants. Cell attachment and spreading on these ligands occur in the absence of extracellular calcium, as does migration on collagen. In contrast, vitronectin-mediated migration is absolutely dependent on the presence of extracellular calcium. Cell contact with immobilized vitronectin or anti-alpha v beta 3 mAbs promotes a measurable ri

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Vukotic, Aleksandra, Jasna Jevdjic, David Green, et al. "Detection of hypotension during spinal anesthesia for caesarean section with continuous non-invasive arterial pressure monitoring and intermittent oscillometric blood pressure monitoring in patients treated with ephedrine or phenylephrine." Srpski arhiv za celokupno lekarstvo 149, no. 7-8 (2021): 442–48. http://dx.doi.org/10.2298/sarh200317030v.

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Introduction/Objective. Despite frequent side effects such as hypotension, spinal anesthesia (SA) is still one of the best anesthetic methods for elective cesarean section (CS). Intermittent, oscillometric, noninvasive blood pressure monitoring (NIBP) frequently leads to missed hypotensive episodes. The objective was to compare continuous non-invasive arterial pressure (CNAP) monitoring with NIBP in the terms of efficiency to detect hypotension. Methods. In this study, we compared CNAP and NIBP monitoring for hypotension detection in 76 patients divided into two groups of 38 patients treated w

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YANG, Yuan-yuan, Jin SHANG, and Hui-guo LIU. "Role of endoplasmic reticular stress in aortic endothelial apoptosis induced by intermittent/persistent hypoxia." Chinese Medical Journal 126, no. 23 (2013): 4517–23. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20130602.

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Background Accumulated evidence shows that hypoxia can induce endothelial apoptosis, however the mechanism is still unknown. We hypothesized whether intermittent or persistent hypoxia could induce endoplasmic reticular stress, leading to endothelial apoptosis. Methods Twenty-four 8-week male Sprague Dawley (SD) rats were divided into three groups: normoxia (NC) group, intermittent hypoxia (IH) group and persistent hypoxia (PH) group. TUNEL staining was performed to detect aortic arch endotheliar apoptosis, and immunohistochemistry for BIP, CHOP and caspase12 to test protein expression; human u

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Raghu, Hari, Neelam Sharma-Walia, Mohanan Valiya Veettil, Sathish Sadagopan, and Bala Chandran. "Kaposi's Sarcoma-Associated Herpesvirus Utilizes an Actin Polymerization-Dependent Macropinocytic Pathway To Enter Human Dermal Microvascular Endothelial and Human Umbilical Vein Endothelial Cells." Journal of Virology 83, no. 10 (2009): 4895–911. http://dx.doi.org/10.1128/jvi.02498-08.

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ABSTRACT Kaposi's sarcoma-associated herpesvirus (KSHV) utilizes clathrin-mediated endocytosis for its infectious entry into human foreskin fibroblast (HFF) cells (S. M. Akula, P. P. Naranatt, N.-S. Walia, F.-Z. Wang, B. Fegley, and B. Chandran, J. Virol. 77:7978-7990, 2003). Here, we characterized KSHV entry into primary human microvascular dermal endothelial (HMVEC-d) and human umbilical vein endothelial (HUVEC) cells. Similar to the results for HMVEC-d cells, KSHV infection of HUVEC cells also resulted in an initial high level and subsequent decline in the expression of the lytic switch gen

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Chen, Guoping, Dongmei Jin, Xuesong Jiang, and Yushan Qiu. "Preparation of Dopamine Nanoparticles and Its Application in the Treatment of Neonatal Scleredema." Science of Advanced Materials 13, no. 6 (2021): 1048–57. http://dx.doi.org/10.1166/sam.2021.4029.

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The polydopamine (PDA) nanoparticles were prepared by the aqueous phase oxidation, and doxorubicin (DOX) was applied on surface of PDA under appropriate temperature and Pondus Hydrogenii (pH) conditions. The human umbilical vein-derived mesenchymal stem cells (hUV-MSCs) were sub-cultured, and then destroyed by lysing and freeze-thaw to obtain the stem cell membrane (SSM) fragments, which were squeezed using the polycarbonate porous membrane and then wrapped in the surfaces of the PDA nanoparticles by means of water bath ultrasound. In this way, the biomimetic nano medicine was synthesized. In

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Wang, Lu, Raffaella Aversa, Zhengjun Houa та ін. "Bioresorption Control and Biological Response of Magnesium Alloy AZ31 Coated with Poly-β-Hydroxybutyrate". Applied Sciences 11, № 12 (2021): 5627. http://dx.doi.org/10.3390/app11125627.

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Magnesium and its alloys are not normally used as bioresorbable temporary implants due to their high and uncontrolled degradation rate in a physiological liquid environment. The improvement of corrosion resistance to simulated body fluids (SBF) of a magnesium alloy (AZ31) coated with poly-β-hydroxybutyrate (PHB) was investigated. Scanning electron microscopy, Fourier transform infrared spectrometer, and contact angle measurements were used to characterize surface morphology, material composition, and wettability, respectively. pH modification of the SBF corroding medium, mass of Mg2+ ions rele

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Xiong, Chenghao, Yingqing Mao, Tao Wu, et al. "Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo." International Journal of Molecular Sciences 19, no. 10 (2018): 2900. http://dx.doi.org/10.3390/ijms19102900.

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Bispecific antibodies, which can bind to two different epitopes on the same or different antigens simultaneously, have recently emerged as attractive candidates for study in various diseases. Our present study successfully constructs and expresses a fully human, bispecific, single-chain diabody (BsDb) that can bind to vascular endothelial growth factor 165 (VEGF165) and programmed death-1 (PD-1) in Pichia pastoris. Under the optimal expression conditions (methanol concentration, 1%; pH, 4.0; inoculum density, OD600 = 4, and the induction time, 96 h), the maximum production level of this BsDb i

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Sporn, L. A., V. J. Marder, and D. D. Wagner. "Differing polarity of the constitutive and regulated secretory pathways for von Willebrand factor in endothelial cells." Journal of Cell Biology 108, no. 4 (1989): 1283–89. http://dx.doi.org/10.1083/jcb.108.4.1283.

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von Willebrand factor (vWf) is secreted from endothelial cells by one of two pathways-a constitutive pathway and a regulated pathway originating from the Weibel-Palade bodies. The molecular form of vWf from each of these pathways differs, with the most biologically potent molecules being released from Weibel-Palade bodies (Loesberg, C., M. D. Gonsalves, J. Zandbergen, C. Willems, W. G. Van Aken, H. V. Stel, J. A. Van Mourik, and P. G. DeGroot. 1983. Biochim. Biophys. Acta. 763:160-168; Sporn, L. A., V. J. Marder, and D. D. Wagner. 1987. Cell. 46:185-190). We investigated the polarity of the tw

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