Gotowe bibliografie tematyczne / Web assembly

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Gotowa bibliografia na temat „Web assembly”

Autor: Grafiati

Data publikacji: 2 czerwca 2025

Data aktualizacji: 5 lipca 2025

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Artykuły w czasopismach na temat "Web assembly"

Contractor, Noshir. "Some assembly required: leveraging Web science to understand and enable team assembly." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 371, no. 1987 (2013): 20120385. http://dx.doi.org/10.1098/rsta.2012.0385.

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Recent advances on the Web have generated unprecedented opportunities for individuals around the world to assemble into teams. And yet, because of the Web, the nature of teams and how they are assembled has changed radically. Today, many teams are ad hoc, agile, distributed, transient entities that are assembled from a larger primordial network of relationships within virtual communities. These assemblages possess the potential to unleash the high levels of creativity and innovation necessary for productively addressing many of the daunting challenges confronting contemporary society. This article argues that Web science is particularly well suited to help us realize this potential by making a substantial interdisciplinary intellectual investment in (i) advancing theories that explain our socio-technical motivations to form teams, (ii) the development of new analytic methods and models to untangle the unique influences of these motivations on team assembly, (iii) harvesting, curating and leveraging the digital trace data offered by the Web to test our models, and (iv) implementing recommender systems that use insights gleaned from our richer theoretical understanding of the motivations that lead to effective team assembly.

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Jiao, Pei Gang. "Research on Remote Virtual Assembly Technology of Plug Valve Based on VRML." Applied Mechanics and Materials 236-237 (November 2012): 526–29. http://dx.doi.org/10.4028/www.scientific.net/amm.236-237.526.

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In this paper, three-dimensional plug valve was created. The function of virtual assembly was achieved using virtual reality modeling language. Network virtual assembly can operate, browse and assemble plug valve remotely by embedding virtual assembly in web pages. The virtual assembly method presented in this paper can be easily extended to deal with many other problems in complex engineering system if control parameters are modified properly.

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Guo, Yulun, Tao Wang, García Molinos Jorge, et al. "Differential Responses of Food Web Properties to Opposite Assembly Rules and Species Richness." Water 12, no. 10 (2020): 2828. http://dx.doi.org/10.3390/w12102828.

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Trophic niches condition the energetic performance of species within food webs providing a vital link between food web assembly, species diversity, and functioning of ecosystems. Our understanding of this important link is, however, limited by the lack of empirical tools that can be easily applied to compare entire food webs at regional scales. By comparison, with different a priori synthetic models defined according to specific assembly rules (i.e., purely random, limiting similarity, and niche filtering), we demonstrate that a set of food web properties (trophic richness, evenness, and divergence) are controlled by ecological processes. We further demonstrate that although both limiting similarity and niche filtering are statistically significant assembly processes shaping our studied lake food webs, their relative importance is richness-dependent, and contextual to the specific food web property under consideration. Our results have both important theoretical and practical implications. Theoretically, the observed richness-dependent variation on food web properties contradicts the common criticism on food web theory that food web properties are roughly scale-invariant. Practically, these properties can help avoiding spurious conclusions, while providing useful information for multiple food web niche spaces supporting the ecosystem functioning.

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Lu, C., Y. S. Wong, and J. Y. H. Fuh. "A web-based assembly planning approach." Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture 222, no. 3 (2008): 427–40. http://dx.doi.org/10.1243/09544054jem948.

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This paper presents a web-based assembly planning approach that enables geographically dispersed planners to carry out the assembly planning task collaboratively and concurrently based on an assembly planning approach using a multiobjective genetic algorithm. Issues in web-based assembly planning are discussed and a workflow is presented. A framework is proposed for web-based assembly planning and the working mechanism is discussed. Finally, a case study is given to verify the proposed approach.

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Jiao, Pei Gang, Shao Fu Shan, and Qiu Hua Miao. "Three-Dimensional Virtual Assembly of Automatic Fuel Measuring Device of Diesel Engine Test Bed Based on VRML." Applied Mechanics and Materials 138-139 (November 2011): 870–73. http://dx.doi.org/10.4028/www.scientific.net/amm.138-139.870.

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In this paper, three-dimensional automatic fuel measuring device of diesel engine test bed was created. The function of virtual assembly was achieved using virtual reality modeling language. Network virtual assembly can operate, browse and assemble automatic fuel measuring device remotely by embedding virtual assembly in web pages. The virtual assembly method presented in this paper can be easily extended to deal with many other problems in engineering system if control parameters are modified properly.

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Kaiser, Rene, Michael Hausenblas, and Martin Umgeher. "Metadata-driven interactive web video assembly." Multimedia Tools and Applications 41, no. 3 (2008): 437–67. http://dx.doi.org/10.1007/s11042-008-0242-z.

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Abhay, Pal. "The Role of Web Assembly in High-Performance Web Applications." Journal of Research and Innovation in Technology, Commerce and Management Vol. 2, Issue 5 (2025): 2431–34. https://doi.org/10.5281/zenodo.15372723.

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WebAssembly (Wasm) is a revolutionary technology in web development, allowing high- performance applications to match native software in speed and efficiency. This paper discusses the role of WebAssembly in improving the performance of web applications through a portable, low-level binary format that runs at near-native speeds. By reviewing its architecture, use cases, and integration with current web technologies, this research points out how WebAssembly closes the gap between web and native performance. The study also looks at existing limitations and suggests future directions for its use in sophisticated, resource-hungry applications. Findings indicate that WebAssembly is a key tool for the future of web-based software, giving developers unprecedented flexibility and power.

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Rahate, Shubham R. "Design of Optimized Trading Strategies with Web Assembly." International Journal for Research in Applied Science and Engineering Technology 9, no. VI (2021): 4997–5001. http://dx.doi.org/10.22214/ijraset.2021.36049.

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Web Assembly is growing and also the most widely studied area which interests many developers when it comes to performance and speed to make web development fast as ever. When it comes to speed and performance algorithms can perform faster computations. Algorithmic trading executes trade at a faster speed. It can buy and sell stocks within a fraction of milliseconds. However, selecting the right tools and technologies is extremely important in algorithmic trading. There are trading strategies which we can use to optimize our trade and increase the return gained on buying and selling stocks. But, choosing an efficient programming language is substantially important. A programming language with a low latency can leverage the trade. Most commonly used languages for algorithmic trading are C/C++, Java, C#, Python. Speed and performance are an essential factor in algorithmic trading. The main purpose of introducing web Assembly in trading as discussed above is speed and performance. Web Assembly is a low-level binary instruction which can execute any program on the web and it can deliver native like performance on the internet. Using Web Assembly, we can compile any code written in languages like C/C++, C#, Java, and python to wasm (Web Assembly executable file) and run on the browser. Web Assembly was developed by W3C, Mozilla Corporation, and Google.

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Song, Wooseok, Hyunjun Jung, and Dongwon Jeong. "Web-based Blockchain Network System Design and Implementation using Web Assembly." Journal of Korean Institute of Information Technology 20, no. 12 (2022): 103–14. http://dx.doi.org/10.14801/jkiit.2022.20.12.103.

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Zhou, Chensheng, Heng Luo, Xiaolu Feng, et al. "FOLDNA, a Web Server for Self-Assembled DNA Nanostructure Autoscaffolds and Autostaples." Journal of Nanotechnology 2012 (2012): 1–5. http://dx.doi.org/10.1155/2012/453953.

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DNA self-assembly is a nanotechnology that folds DNA into desired shapes. Self-assembled DNA nanostructures, also known as origami, are increasingly valuable in nanomaterial and biosensing applications. Two ways to use DNA nanostructures in medicine are to form nanoarrays, and to work as vehicles in drug delivery. The DNA nanostructures perform well as a biomaterial in these areas because they have spatially addressable and size controllable properties. However, manually designing complementary DNA sequences for self-assembly is a technically demanding and time consuming task, which makes it advantageous for computers to do this job instead. We have developed a web server, FOLDNA, which can automatically design 2D self-assembled DNA nanostructures according to custom pictures and scaffold sequences provided by the users. It is the first web server to provide an entirely automatic design of self-assembled DNA nanostructure, and it takes merely a second to generate comprehensive information for molecular experiments including: scaffold DNA pathways, staple DNA directions, and staple DNA sequences. This program could save as much as several hours in the designing step for each DNA nanostructure. We randomly selected some shapes and corresponding outputs from our server and validated its performance in molecular experiments.

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Rozprawy doktorskie na temat "Web assembly"

Perez, De Rosso Santiago(Santiago Nicolas). "Declarative assembly of web applications from predefined concepts." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/128295.

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This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2020 Cataloged from student-submitted PDF of thesis. Includes bibliographical references (pages 181-186). This thesis presents a new approach to web application development, in which an application is constructed by configuring and composing concepts drawn from a catalog developed by experts. A concept is a self-contained, reusable unit of behavior that is motivated by a purpose defined in terms of the needs of an end-user. Each concept includes both client- and server-side functionality and exports a collection of components--graphical user interface elements, backed by application logic and database storage. To build a web application, the developer imports concepts from the catalog, tunes them to fit the needs of the application via configuration variables, and links concept components together to create pages. Components of different concepts may be executed independently or bound together declaratively with dataflows and synchronization. The instantiation, configuration, linking and binding of components is all expressed in a simple template language. The approach has been implemented in a platform called Déjà Vu. We outline and compare our approach to conventional approaches to web application development and we present results from a case study in which we used our platform to replicate a collection of applications previously built by students for a web programming course. by Santiago Perez De Rosso. Ph. D. Ph.D. Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science

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Drangsholt, Finn. "The applicability of demand controlled ventilating systems for assembly halls /." Online version, 1992. http://bibpurl.oclc.org/web/29597.

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Greig, Hamish Stuart. "Community assembly and food web interactions across pond permanence gradients." Thesis, University of Canterbury. School of Biological Sciences, 2008. http://hdl.handle.net/10092/1585.

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Ecological communities along gradients of environmental stress are thought to be structured by trade-offs between resisting biotic interactions in physically benign habitats and successfully exploiting physically stressful habitats. However, these trade-offs are likely to be affected by the predictability of abiotic stressors, and variation in the strength of biotic interactions. I investigated community assembly and food web interactions in ponds across an unpredictable gradient of water inundation (pond permanence) in Canterbury, New Zealand. Pond community composition and species richness were strongly influenced by pond permanence. However, species in temporary ponds were a nested subset of generalists that were also found in permanent ponds, rather than a unique assemblage of temporary pond specialists. Subsequent experiments indicated predator impact decreased with pond permanence, partially due to the foraging suppression of predatory invertebrates in permanent ponds by fish. Weak predation in permanent ponds combined with unpredictable drying regimes likely selected for generalist traits, and resulted in community assembly being driven by a gradient of drying stress rather than trade-offs between biotic interactions and drying. Furthermore, predator impact increased over time in temporary ponds. In predictable snow-melt ponds in the Colorado Rocky Mountains, seasonal windows of weak predation were exploited by vulnerable species, leading to increased diversity within habitats. However in unpredictable systems like Canterbury, temporal increases in predation risk that depend on drying history are likely to increase variability in the spatial arrangement of suitable habitats for particular species. This should further favour the evolution of generalist traits and reduce the importance of trade-offs between predation and drying in the assembly of communities. Considering the predictability of disturbance regimes and the spatial and temporal variation in biotic interactions will greatly enhance understanding and management of communities in heterogeneous landscapes.

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nnah, Marie Hannah Marie. "The effects of assembly method on food webs : integrating population dynamics, evolution and web size." Thesis, University of York, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516620.

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Oliveira, Matheus Brito de. "GATOOL - Genome Assembly Tool: uma ferramenta web para montagem de genomas bacterianos." Universidade Estadual de Feira de Santana, 2017. http://localhost:8080/tede/handle/tede/513.

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Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-10-09T22:34:41Z No. of bitstreams: 1 MATHUES BRITO DE OLIVEIRA Disserta??ov.pdf: 5287293 bytes, checksum: 8d3e3b854b5799f16c0b61b6a5d33f1c (MD5) Made available in DSpace on 2017-10-09T22:34:41Z (GMT). No. of bitstreams: 1 MATHUES BRITO DE OLIVEIRA Disserta??ov.pdf: 5287293 bytes, checksum: 8d3e3b854b5799f16c0b61b6a5d33f1c (MD5) Previous issue date: 2017-06-12 The assembly of bacterial genomes consists of a process of reordering fragments so that the original genome can be represented. However, to maximize the results of genome assembly, some steps are required, for instance, read quality analysis and preprocessing, repetition identification and quality check. The process of assembly of genomes is a complex step that involves the type of sequencing that was used, there are several types of sequencers which imply different characteristics for each one for example: fragments size, throughput, among others. Analyzing these characteristics requires the use of several computational tools, to assist in all the processes mentioned above, and since the range of software available is quite broad and distinct, it is necessary for the user to learn to work with this computational diversity, dominating often knowledge that is not of the biological area, implying in less time for a deepening in biological questions. Based on this context, we developed a pipeline to perform an automated fragment analysis, read preprocessing, genome assembly and orientation of contigs, having as the assembly the main objective of the pipeline and that it will be managed by a Web application called GATOOL (Genome Assembly Tool). Aiming to evaluate the performance of the application, tests were carried out with two samples of prokaryotic organisms, which are: Bacillus amyloliquefaciens and Serratia marcescens. Also perform a test with seven SRA samples. Both organisms are sequenced on the Ion PGMTM platform. The tools used to perform the assembly were SPAdes and Velvet, both assemblers use de Bruijn graph algorithm as a paradigm for the assembly of the genome, after this stage the resulting set of contigs was ordered through the CONTIGuator, which is a reference ordering. We observed that the interface GATOOL allowed a quick and easy execution of several steps and processes in the field of genome assembly, including the assembly of two prokaryotic species in an automated way, thus facilitating the use and accomplishment of such processes by any user. A montagem de genomas bacterianos ? um processo de reordena??o de fragmentos, de forma que se possa representar o genoma original. Entretanto, para que a montagem de um genoma seja realizada visando maximizar os resultados, ? preciso que algumas etapas sejam cumpridas, por exemplo: a an?lise dos fragmentos, o pr?-processamento destes fragmentos e novamente uma repeti??o do processo de an?lise, para verificar a efic?cia do pr?-processamento realizado. O processo de montagem de genomas ? uma etapa complexa, que envolve o tipo de sequenciamento que foi utilizado. Existem diversos tipos de sequenciadores, o que implica caracter?sticas distintas em cada um, como por exemplo: tamanho dos fragmentos, quantidade de fragmentos gerados por corrida, dentre outros. Analisando essas caracter?sticas, faz-se necess?ria a utiliza??o de diversas ferramentas computacionais para auxiliar a todos os processos citados anteriormente e, como a gama de softwares dispon?veis ? bem ampla e distinta, ? importante que o usu?rio domine essa diversidade computacional, contendo muitas vezes conhecimentos que n?o s?o da ?rea biol?gica, implicando menos tempo para um aprofundamento das quest?es biol?gicas. Com base neste contexto, prop?em-se um pipeline para a realiza??o da an?lise de fragmentos, pr?-processamento dos fragmentos, montagem de genomas e orienta??o de contigs, tendo como a montagem o objetivo principal do pipeline e este ser? gerenciado por uma aplica??o web chamada GATOOL (Genome Assembly Tool). Visando avaliar o desempenho da aplica??o, foram feitos testes com duas amostras de organismos procariontes, que s?o: Bacillus amyloliquefaciens e Serratia marcescens. Tamb?m foram realizados testes com sete amostras SRA. Ambos os organismos est?o sequenciados na plataforma Ion PGMTM. Os montadores usados foram o SPAdes e o Velvet, ambos montadores, utilizam o algor?tmo grafo de Bruijn como paradigma para a montagem do genoma; ap?s esta etapa, o conjunto de contigs resultante foi ordenado atrav?s do CONTIGuator, que ? uma ordena??o por refer?ncia. Observamos que a interface GATOOL permitiu uma execu??o r?pida e f?cil de diversas etapas e processos no campo da montagem de genomas, inclusive realizando a montagem de duas esp?cies procariontes de maneira automatizada, facilitando assim a utiliza??o e realiza??o de tais processos por qualquer usu?rio.

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Thimmapuram, Sunethra. "Web-Delivered Assembly Language Interactive Training and its Sequence Identification for Software Reverse Engineering." Wright State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright151551307959276.

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Alpaslan, Mehmet. "The cosmic web unravelled : a study of filamentary structure in the Galaxy and Mass Assembly survey." Thesis, University of St Andrews, 2014. http://hdl.handle.net/10023/4906.

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I have investigated the properties of the large scale structure of the nearby Universe using data from the Galaxy and Mass Assembly survey (GAMA). I generated complementary halo mass estimates for all groups in the GAMA Galaxy Group Catalogue (G³C) using a modified caustic mass estimation algorithm. On average, the caustic mass estimates agree with dynamical mass estimates within a factor of 2 in 90% of groups. A volume limited sample of these groups and galaxies are used to generate the large scale structure catalogue. An adapted minimal spanning tree algorithm is used to identify and classify structures, detecting 643 filaments that measure up to 200 Mpc/h, each containing 8 groups on average. A secondary population of smaller coherent structures, dubbed `tendrils,' that link filaments together or penetrate into voids are also detected. On average, tendrils measure around 10 Mpc/h and contain 6 galaxies. The so-called line correlation function is used to prove that tendrils are real structures rather than accidental alignments. A population of isolated void galaxies are also identified. The properties of filaments and tendrils in observed and mock GAMA galaxy catalogues agree well. I go on to show that voids from other surveys that overlap with GAMA regions contain a large number of galaxies, primarily belonging to tendrils. This implies that void sizes are strongly dependent on the number density and sensitivity limits of the galaxies observed by a survey. Finally, I examine the properties of galaxies in different environments, finding that galaxies in filaments tend to be early-type, bright, spheroidal, and red whilst those in voids are typically the opposite: blue, late-type, and more faint. I show that group mass does not correlate with the brightness and morphologies of galaxies and that the primary driver of galaxy evolution is stellar mass.

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Sutanto, Agus. "Solution approaches for planning of assembly systems in three-dimensional virtual environments /." Bamberg : Meisenbach, 2005. http://deposit.ddb.de/cgi-bin/dokserv?id=2650819&prov=M&dok_var=1&dok_ext=htm.

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Yeager, Lauren. "Does Landscape Context Affect Habitat Value? The Importance of Seascape Ecology in Back-reef Systems." FIU Digital Commons, 2013. http://digitalcommons.fiu.edu/etd/892.

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Seascape ecology provides a useful framework from which to understand the processes governing spatial variability in ecological patterns. Seascape context, or the composition and pattern of habitat surrounding a focal patch, has the potential to impact resource availability, predator-prey interactions, and connectivity with other habitats. For my dissertation research, I combined a variety of approaches to examine how habitat quality for fishes is influenced by a diverse range of seascape factors in sub-tropical, back-reef ecosystems. In the first part of my dissertation, I examined how seascape context can affect reef fish communities on an experimental array of artificial reefs created in various seascape contexts in Abaco, Bahamas. I found that the amount of seagrass at large spatial scales was an important predictor of community assembly on these reefs. Additionally, seascape context had differing effects on various aspects of habitat quality for the most common reef species, White grunt Haemulon plumierii. The amount of seagrass at large spatial scales had positive effects on fish abundance and secondary production, but not on metrics of condition and growth. The second part of my dissertation focused on how foraging conditions for fish varied across a linear seascape gradient in the Loxahatchee River estuary in Florida, USA. Gray snapper, Lutjanus griseus, traded food quality for quantity along this estuarine gradient, maintaining similar growth rates and condition among sites. Additional work focused on identifying major energy flow pathways to two consumers in oyster-reef food webs in the Loxahatchee. Algal and microphytobenthos resource pools supported most of the production to these consumers, and body size for one of the consumers mediated food web linkages with surrounding mangrove habitats. All of these studies examined a different facet of the importance of seascape context in governing ecological processes occurring in focal habitats and underscore the role of connectivity among habitats in back-reef systems. The results suggest that management approaches consider the surrounding seascape when prioritizing areas for conservation or attempting to understand the impacts of seascape change on focal habitat patches. For this reason, spatially-based management approaches are recommended to most effectively manage back-reef systems.

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Hulett, Maria. "Analytical Approximations to Predict Performance Measures of Manufacturing Systems with Job Failures and Parallel Processing." FIU Digital Commons, 2010. http://digitalcommons.fiu.edu/etd/167.

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Parallel processing is prevalent in many manufacturing and service systems. Many manufactured products are built and assembled from several components fabricated in parallel lines. An example of this manufacturing system configuration is observed at a manufacturing facility equipped to assemble and test web servers. Characteristics of a typical web server assembly line are: multiple products, job circulation, and paralleling processing. The primary objective of this research was to develop analytical approximations to predict performance measures of manufacturing systems with job failures and parallel processing. The analytical formulations extend previous queueing models used in assembly manufacturing systems in that they can handle serial and different configurations of paralleling processing with multiple product classes, and job circulation due to random part failures. In addition, appropriate correction terms via regression analysis were added to the approximations in order to minimize the gap in the error between the analytical approximation and the simulation models. Markovian and general type manufacturing systems, with multiple product classes, job circulation due to failures, and fork and join systems to model parallel processing were studied. In the Markovian and general case, the approximations without correction terms performed quite well for one and two product problem instances. However, it was observed that the flow time error increased as the number of products and net traffic intensity increased. Therefore, correction terms for single and fork-join stations were developed via regression analysis to deal with more than two products. The numerical comparisons showed that the approximations perform remarkably well when the corrections factors were used in the approximations. In general, the average flow time error was reduced from 38.19% to 5.59% in the Markovian case, and from 26.39% to 7.23% in the general case. All the equations stated in the analytical formulations were implemented as a set of Matlab scripts. By using this set, operations managers of web server assembly lines, manufacturing or other service systems with similar characteristics can estimate different system performance measures, and make judicious decisions - especially setting delivery due dates, capacity planning, and bottleneck mitigation, among others.

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Książki na temat "Web assembly"

Commonwealth Competition Council (Va.), ed. Plan for the development of a system and review process to implement and maintain a statewide database of nonprofit and not-for-profit entities that could be accessed by all Virginians via the Internet: Report of the Commonwealth Competition Council to the Governor and the General Assembly of Virginia. Commonwealth of Virginia, 2002.

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Anderson, Gail. Assemble the social web with zembly. Prentice Hall, 2009.

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1949-, Anderson Paul, ed. Assemble the social web with zembly. Prentice Hall, 2009.

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Macau (China : Special Administrative Region). Li fa hui., ed. Ji hui quan ji shi wei quan. Aomen Tebie Xingzhengqu li fa hui, 2001.

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Virginia. Secretary of Health and Human Resources. An action plan prescribing renewed partnerships among the Center for Information [i.e., Innovative] Technology, the Department of Information Technology and the disability services agencies: Report of the Secretary of Health and Human Resources and the Secretary of Technology to the Governor and the General Assembly of Virginia. Commonwealth of Virginia, 2003.

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Catholic Church. Deutsche Bischofskonferenz. Sekretariat., ed. "--sie zogen auf einem anderen Weg heim in ihr Land" (Mt 2, 12): Ein Weltjugendtag, der länger wirkt, als er dauert : Handreichung für die Nachbereitung des XX. Jugendtags 2005, 22. August 2005. Sekretariat der Deutschen Bischofskonfenenz, 2005.

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Borzyh, Stanislav. Theory of the possible. INFRA-M Academic Publishing LLC., 2021. http://dx.doi.org/10.12737/1074108.

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In this book, we are talking about a single principle that permeates every organized entity, regardless of what sphere it belongs to. Everywhere and always, and in accordance with the current circumstances, the process of formation, support and regulation of any complex complexes and ensembles is guided and controlled by the concept of the realizable, which postulates that only what is stable and stable will be realized, and everything else will be discarded as untenable and unbalanced. These patterns and patterns can be traced resolutely at all levels of existence. And the universe, and life, and consciousness, and mind, and culture are arranged and assembled according to these schemes, because it is difficult, if possible, for them to be any other. This paper provides an overview of this type of layout in these areas, as well as the theory of the achievable and accessible itself. Using examples and theoretical considerations, it is shown that the configuration of all reliable and long-lasting structures is approximately the same or very similar, because it obeys a single end-to-end logic of the formation of any similar substances, whatever they touch and wherever they are found. In addition, it is demonstrated that if something in this spirit is objectified in practice, then its nature and properties must be fundamentally the same as what we observe around or extremely close to it. Finally, the view is argued and developed, according to which everything consists of matter, is constituted by it, is reduced only to it, including any non-physical phenomena. It is concluded that all the wealth of the world is subject to the same laws of its construction, and all this construction observes the universal rules of the functioning of complex things, no matter what they are aimed at. For all those interested in philosophy.

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United States. Congress. House. Committee on International Relations. Subcommittee on Asia and the Pacific. A resolution concerning Burma and the United Nations General Assembly and a concurrent resolution concerning writer, political philosopher, human rights advocate, and Nobel Peace Prize nominee Wei Jingsheng: Markup before the Subcommittee on Asia and the Pacific, Committee on International Relations, and before the Committee on International Relations (H. Con. Res. 117 only), House of Representatives, One Hundred Fourth Congress, first session, on H. Res. 274 and H. Con. Res. 117, December 6 and 7, 1995. U.S. G.P.O., 1997.

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Anderson, Paul, Chris Webster, Gail Anderson, and Todd Fast. Assemble the Social Web with Zembly. Pearson Education, Limited, 2021.

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Assemble the Social Web with Zembly. Pearson Education, Limited, 2009.

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Części książek na temat "Web assembly"

Freeman, Adam. "Using Blazor Web Assembly." In Pro ASP.NET Core 3. Apress, 2020. http://dx.doi.org/10.1007/978-1-4842-5440-0_37.

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Freeman, Adam. "Using Blazor Web Assembly." In Pro ASP.NET Core 6. Apress, 2022. http://dx.doi.org/10.1007/978-1-4842-7957-1_37.

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Bruyat, Julian, Pierre-Antoine Champin, Lionel Médini, and Frédérique Laforest. "WasmTree: Web Assembly for the Semantic Web." In The Semantic Web. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-77385-4_35.

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Tres, Paul A. "World Wide Web References Related to Plastic Part Design." In Designing Plastic Parts for Assembly, 9th ed. Carl Hanser Verlag GmbH & Co. KG, 2021. http://dx.doi.org/10.3139/9781569908211.017.

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Lu, Mian, Qiong Luo, Bingqiang Wang, Junkai Wu, and Jiuxin Zhao. "GPU-Accelerated Bidirected De Bruijn Graph Construction for Genome Assembly." In Web Technologies and Applications. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37401-2_8.

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Huang, George Q., and K. L. Mak. "Web-Based Design for Manufacture and Assembly." In Internet Applications in Product Design and Manufacturing. Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55778-1_8.

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Zeng, Li, Jiefeng Cheng, Jintao Meng, Bingqiang Wang, and Shengzhong Feng. "Improved Parallel Processing of Massive De Bruijn Graph for Genome Assembly." In Web Technologies and Applications. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37401-2_12.

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Choi, Kyung-Hyun, Tran Trung Thanh, Yang Bong Su, and Dong-Soo Kim. "Printing Pressure Control Algorithm of Roll-to-Roll Web System for Printed Electronics." In Frontiers of Assembly and Manufacturing. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14116-4_16.

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Murphy, Seán, Leonardas Persaud, William Martini, and Bill Bosshard. "On the Use of Web Assembly in a Serverless Context." In Agile Processes in Software Engineering and Extreme Programming – Workshops. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-58858-8_15.

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Abstract This paper considers how WASM can be run in different serverless contexts. A comparison of different serverside WASM runtime options is considered, specifically focused on , and . Next, different options for running WASM within two serverless platforms – Openwhisk and AWS Lambdai – are compared. Initial results show that a solution which uses the built-in WASM supports is found to work better than using the dedicated WASM runtimes but this has limitations and providing more direct integration with WASM runtimes should be explored further.

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Jovanović, Jelena, Dragan Gašević, and Vladan Devedžić. "Dynamic Assembly of Personalized Learning Content on the Semantic Web." In Lecture Notes in Computer Science. Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/11762256_40.

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Streszczenia konferencji na temat "Web assembly"

Hsu, Edwin, Nancy Huang, Leo Lee, William Hung, and Natascha Zhang. "Automatically Generate SMT Mounter Production Data with Python Web Scraping." In 2024 19th International Microsystems, Packaging, Assembly and Circuits Technology Conference (IMPACT). IEEE, 2024. https://doi.org/10.1109/impact63555.2024.10818876.

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Mao, Chenxi, Yuxin Su, Shiwen Shan, and Dan Li. "eWAPA: An eBPF-based WASI Performance Analysis Framework for Web Assembly Runtimes." In 2024 IEEE International Conference on Software Services Engineering (SSE). IEEE, 2024. http://dx.doi.org/10.1109/sse62657.2024.00054.

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Hirohata, Mikihito, Aoi Utou, Kotaro Murabayashi, Károly Jármai, Jun Ito, and Seiji Okada. "A Proposal of I-section Girder Assembled by Dissimilar Steels with Different Strengths." In IABSE Symposium, Tokyo 2025: Environmentally Friendly Technologies and Structures: Focusing on Sustainable Approaches. International Association for Bridge and Structural Engineering (IABSE), 2025. https://doi.org/10.2749/tokyo.2025.1777.

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A series of investigations were conducted to propose an I-section girder assembled by dissimilar steels with different strengths for utilizing general structural steel and newly developed high- performance steel (Steels for Bridge High-performance Structures: SBHS). As the basic concept of the section subjected to bending loads, the upper flange and the upper side of the web were composed of ordinary steel with 320 N/mm² of yield strength (SM400) while the lower flange and the lower side of the web were composed of high-strength steels with 454, 535, and 849 N/mm² of yield strengths (SBHS400, SBHS500, SBHS700), respectively. The flanges and web dimensions were optimized to achieve high bending strength, high stiffness, and lightweight. The yield moment and the stiffness of the combined I-girder with dissimilar steels under the bending loads could be improved from those of the reference I-girder composed of ordinary steel only.

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Tiwary, Mayank, Pritish Mishra, Shashank Jain, and Deepak Puthal. "Data Aware Web-Assembly Function Placement." In WWW '20: The Web Conference 2020. ACM, 2020. http://dx.doi.org/10.1145/3366424.3382670.

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Farrell, Robert G., Soyini D. Liburd, and John C. Thomas. "Dynamic assembly of learning objects." In the 13th international World Wide Web conference. ACM Press, 2004. http://dx.doi.org/10.1145/1013367.1013394.

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Bailey, Donald G. "Interactive Web-based tutorials for teaching digital electronics." In International Symposium on Microelectronics and Assembly, edited by Bernard Courtois, Serge N. Demidenko, and Lee Y. Lau. SPIE, 2000. http://dx.doi.org/10.1117/12.405415.

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Perez De Rosso, Santiago, Daniel Jackson, Maryam Archie, Czarina Lao, and Barry A. McNamara III. "Declarative assembly of web applications from predefined concepts." In SPLASH '19: 2019 ACM SIGPLAN International Conference on Systems, Programming, Languages, and Applications: Software for Humanity. ACM, 2019. http://dx.doi.org/10.1145/3359591.3359728.

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Qamar, Atif, and Zahed Siddique. "An Interactive Web-Based 3D Environment for Assembly Training." In ASME 2008 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/detc2008-49695.

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The role of multimedia enriched web-based applications is being widely accepted as an effective training and instruction mechanism for technical training. Individualized training tools can be created to fit the trainee’s needs. The use of three-dimensional visualization for the web-based training environments plays a key role in building an effective and highly interactive training application. There are still needs to overcome problems regarding the integration of multimedia objects such as three-dimensional animation and simulation. The main objective of this research is to develop the training system with the help of the latest multimedia tools and using the internet as a delivery media. The use of the three-dimensional visualization adds a significant improvement in web-based environment for technical and engineering trainings. In order to accomplish the goals of this research, two kinds of environments are established. Although, the training sections of both the environments are same, there is a difference in terms of editing the position and appearance of the solid models inside the training environment. In the first environment, there is always need of multimedia specialist to edit the position and appearance of the solid models inside the environment. Second environment is provided with a text-based HTML file to provide the easy editing tool for controlling the solid models inside the training environment.

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Pahl, Claus, and Ronan Barrett. "A web services architecture for learning object discovery and assembly." In the 13th international World Wide Web conference. ACM Press, 2004. http://dx.doi.org/10.1145/1013367.1013518.

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Zhang, Qiong, Congfu Xu, and Jianzhong Mo. "Web-based virtual assembly/disassembly system for e-commerce." In Second International Conference on Image and Graphics, edited by Wei Sui. SPIE, 2002. http://dx.doi.org/10.1117/12.477091.

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Raporty organizacyjne na temat "Web assembly"

Seybold, Patricia. What to Look for in a Web Services Assembly Platform. Patricia Seybold Group, 2002. http://dx.doi.org/10.1571/psgp1-10-02cc.

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Friedmann, Michael, Charles J. Arntzen, and Hugh S. Mason. Expression of ETEC Enterotoxin in Tomato Fruit and Development of a Prototype Transgenic Tomato for Dissemination as an Oral Vaccine in Developing Countries. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7585203.bard.

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The broad objective of the project was to develop a feasible approach to combat diarrheal disease caused by ETEC through the development of a low-cost oral immunogen in tomato fruit, expressed in the context of a prototype tomato that would answer the shortcomings of plant oral vaccines, especially in terms of produce handling and control of gene escape. Specifically, the goals for Boyce Thompson Institute (BTI) on this project were to develop transgenic tomato lines that express the enterotoxigenic E. coli (ETEC) heat-labile enterotoxin (LT) subunits A and/or B for use in oral edible vaccines, and to optimize expression and assembly of these antigens in tomato fruits.LT-B is a useful vaccine antigen against ETEC disease, since antibodies against LT-B can prevent binding and delivery of the holotoxinLT. Mutant forms of the toxic LT-A subunit that have reduced toxicity can be co-expressed and assembled with LT-Bpentamers to form mutant LT (mLT) complexes that could be used as mucosaladjuvants for other oral vaccines. Work on the project is continuing at Arizona State University, after Dr. Mason moved there in August 2002. A number of approaches were taken to ensure the expression of both subunits and bring about their assembly inside the transgenic fruits. Initially, expression was driven by the fruit-specific E-8 promoter for LT-B and the constitutive CaMV 35S promoter for LT-A(K63). While LT-B accumulated up to 7 µg per gram ripe fruit, assembled LT-K63 was only 1 µg per gram. Since promoter activities for the two genes likely differed in cell type and developmental stage specificity, the ratios of A and B subunits was not optimal for efficient assembly in all cells. In order to maximize the chance of assembly of mLT in fruit, we focused on constructs in which both genes are driven by the same promoter. These included co-expression plasmids using the 35S promoter for both, while switching to attenuated mLTs (LT-R72 and LT-G192) that have shown greater potential for oral adjuvanticity than the initial LT-K63, and thus are better candidates for a plant-derived adjuvant. Other, more novel approaches were then attempted, including several new vectors using the tomato fruit-specific E8 promoter driving expression of both LT-B and mutant LT-A, as well as a dicistronic construct for co-expression of both LT-B and mutant LT-A genes from a single promoter, and a geminivirusreplicon construct. We describe in the Appendix the results obtained in transgenic tomato lines transformed with these constructs. Overall, each contributed to enhanced expression levels, but the assembly itself of the holotoxin to high levels was not observed in the fruit tissues. The Israeli lab’s specific objective was to develop transgenic tomato lines expressing the LTholotoxin antigen bearing attributes to prevent gene escape (male sterility and orange fruit color) and to improve the dissemination of the oral vaccine (long shelf-life tomato cherry fruit or tomato processing background). Breeding lines bearing a number of attributes to prevent gene escape were developed by combining material and backcrossing either to a tomato cherry background, or two different processing backgrounds. Concomitantly, (these lines can be utilized for the creation of any future oral vaccine or other therapeutic-expressing tomato, either by crosses or transformation), the lines were crossed to the holotoxin-expressing tomatoes received from the United States, and this transgenic material was also incorporated into the backcrossing programs. To date, we have finalized the preparation of the cherry tomato material, both non-transgenic (bearing all the desired attributes), and transgenic, expressing the holotoxin. The level of expression of LT-B in the cherry fruits was comparable to the original transgenic tomatoes. Since it was not higher, this would necessitate the consumption of more fruits to reach a desired dose. A final backcross has been made for both the non-transgenic and the transgenic material in the processing lines. Auxin sprays resulted in high percentages of fruit set, but the processing genotypes gave many puffed fruits.

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Galili, Gad, and Alan Bennett. Role of Molecular Chaperone in Wheat Storage Protein Assembly. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604926.bard.

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Following sequestration into the ER, wheat gliadins assemble into complexes that initiate the formation of protein bodies. In the present work we have characterized the DNA sequence and regulation of expression of a plant BiP and also studied its interaction with wheat storage proteins as well as its role in the maturation of these storage proteins. In the Israeli lab, immunoprecipitation studies were made using anti BiP and anti storage proteins sera, both in wheat and in transgenic tobacco plants expressing a wheat gliadin storage proteins. In both cases, we could show that BiP interacts with the gliadin storage proteins. In addition, we could show that BiP also played an important role in the initial assembly of the gliadins. In the American lab, the complexity, structure and properties of tomato BiP was characterized at the molecular and biochemical levels. In addition, tomato BiP was also overexpressed in bacteria and the overexpressed protein was found to be active. The cooperative findings of the Israeli and American labs clearly improves our understanding of the structure and expression of a plant BiP as well as its role in the maturation of storage proteins in plants seeds. In addition, it will serve as a foundation for future studies of the mechanisms of BiP function in in vitro studies using purified storage proteins and purified recombinant active BiP.

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Herman, Eliot D., Gad Galili, and Alan Bennett. Recognition and Disposal of Misfolded Seed Proteins. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568791.bard.

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This project was directed at determining mechanisms involved in storage of intrinsic and foreign storage proteins in seeds. Seeds constitute the majority of direct and indirect food. Understanding how seeds store proteins is important to design approaches to improve the quality of seed proteins through biotechnology. In the Israeli part of this project we have conducted investigations to elucidate the mechanisms involved in assembling wheat storage proteins into ER-derived protein bodies. The results obtained have shown how domains of storage protein molecules are critical in the assembly of protein bodies. In the US side of this project the fate of foreign and engineered proteins expressed in seeds has been investigated. Engineering seed proteins offers the prospect of improving the quality of crops. Many foreign proteins are unstable when expressed in transgenic seeds. The results obtained have demonstrated that sequestering foreign proteins in the ER or ER-derived protein bodies stabilizes the proteins permitting their accumulation. The collaboration conducted in this project has advanced the understanding how protein bodies are assembled and the potential to use the ER and protein bodies to store engineered proteins that can enhance the composition of seeds.

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Nelson, Nathan, and Randy Schekman. Functional Biogenesis of V-ATPase in the Vacuolar System of Plants and Fungi. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7574342.bard.

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The vacuolar H+-ATPase (V-ATPase) is one of the most fundamental enzymes in nature. It pumps protons into the vacuolar system of eukaryotic cells and provides the energy for numerous transport systems. Through our BARD grant we discovered a novel family of membrane chaperones that modulate the amount of membrane proteins. We also elucidated the mechanism by which assembly factors guide the membrane sector of V-ATPase from the endoplasmic reticulum to the Golgi apparatus. The major goal of the research was to understand the mechanism of action and biogenesis of V-ATPase in higher plants and fungi. The fundamental question of the extent of acidification in organelles of the vacuolar system was addressed by studying the V-ATPase of lemon fruit, constructing lemon cDNAs libraries and study their expression in mutant yeast cells. The biogenesis of the enzyme and its function in the Golgi apparatus was studied in yeast utilizing a gallery of secretory mutants available in our laboratories. One of the goals of this project is to determine biochemically and genetically how V-ATPase is assembled into the different membranes of a wide variety of organelles and what is the mechanism of its action.The results of this project advanced out knowledge along these lines.

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Steffens, John C., and Eithan Harel. Polyphenol Oxidases- Expression, Assembly and Function. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7571358.bard.

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Polyphenol oxidases (PPOs) participate in the preparation of many plant products on the one hand and cause considerable losses during processing of plant products on the other hand. However, the physiological functions of plant PPO were still a subject of controversy at the onset of the project. Preliminary observations that suggested involvement of PPOs in resistance to herbivores and pathogens held great promise for application in agriculture but required elucidation of PPO's function if modulation of PPO expression is to be considered for improving plant protection or storage and processing of plant products. Suggestions on a possible role of PPO in various aspects of chloroplast metabolism were also relevant in this context. The characterization of plant PPO genes opened a way for achieving these goals. We reasoned that "understanding PPO targeting and routing, designing ways to manipulate its expression and assessing the effects of such modifications will enable determination of the true properties of the enzyme and open the way for controlling its activity". The objective of the project was to "obtain an insight into the function and biological significance of PPOs" by examining possible function(s) of PPO in photosynthesis and plant-pest interactions using transgenic tomato plants; extending our understanding of PPO routing and assembly and the mechanism of its thylakoid translocation; preparing recombinant PPOs for use in import studies, determination of the genuine properties of PPOs and understanding its assembly and determining the effect of PPO's absence on chloroplast performance. Results obtained during work on the project made it necessary to abandon some minor objectives and devote the effort to more promising topics. Such changes are mentioned in the 'Body of the report' which is arranged according to the objectives of the original proposal. The complex expression pattern of tomato PPO gene family was determined. Individual members of the family are differentially expressed in various parts of the plant and subjected to developmentally regulated turnover. Some members are differentially regulated also by pathogens, wounding and chemical wound signals. Wounding systemically induces PPO activity and level in potato. Only tissues that are developmentally competent to express PPO are capable of responding to the systemic wounding signal by increased accumulation of PPO mRNA. Down regulation of PPO genes causes hyper susceptibility to leaf pathogens in tomato while over expression regulation of PPO expression in tomato plants is their apparent increased tolerance to drought. Both the enhanced disease resistance conferred by PPO over expression and the increased stress tolerance due to down regulation can be used in the engineering of improved crop plants. Photosynthesis rate and variable fluorescence measurements in wild type, and PPO-null and over expressing transgenic tomato lines suggest that PPO does not enable plants to cope better with stressful high light intensities or reactive oxygen species. Rather high levels of the enzyme aggravate the damage caused under such conditions. Our work suggests that PPO's primary role is in defending plants against pathogens and herbivores. Jasmonate and ethylene, and apparently also salicylate, signals involved in responses to wounding and defense against herbivores and pathogens, enhance markedly and specifically the competence of chloroplasts to import and process pPPO. The interaction of the precursor with thylakoid membranes is primarily affected. The routing of PPO shows other unusual properties: stromal processing occurs in two sites, resulting in intermediates that are translocated across thylakoids by two different mechanisms - a DpH- and a Sec-dependent one. It is suggested that the dual pattern of processing and routing constitutes a'fail safe' mechanism, reflecting the need for a rapid and flexible response to defense challenges. Many of the observations described above should be taken into consideration when manipulation of PPO expression is contemplated for use in crop improvement.

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Wilson, Thomas E., Avraham A. Levy, and Tzvi Tzfira. Controlling Early Stages of DNA Repair for Gene-targeting Enhancement in Plants. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697124.bard.

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Gene targeting (GT) is a much needed technology as a tool for plant research and for the precise engineering of crop species. Recent advances in this field have shown that the presence of a DNA double-strand break (DSB) in a genomic locus is critical for the integration of an exogenous DNA molecule introduced into this locus. This integration can occur via either non-homologous end joining (NHEJ) into the break or homologous recombination (HR) between the broken genomic DNA and the introduced vector. A bottleneck for DNA integration via HR is the machinery responsible for homology search and strand invasion. Important proteins in this pathway are Rad51, Rad52 and Rad54. We proposed to combine our respective expertise: on the US side, in the design of zincfinger nucleases (ZFNs) for the induction of DNA DSBs at any desired genomic locus and in the integration of DNA molecules via NHEJ; and on the Israeli side in the HR events, downstream of the DSB, that lead to homology search and strand invasion. We sought to test three major pathways of targeted DNA integration: (i) integration by NHEJ into DSBs induced at desired sites by specially designed ZFNs; (ii) integration into DSBs induced at desired sites combined with the use of Rad51, Rad52 and Rad54 proteins to maximize the chances for efficient and precise HR-mediated vector insertion; (iii) stimulation of HR by Rad51, Rad52 and Rad54 in the absence of DSB induction. We also proposed to study the formation of dsT-DNA molecules during the transformation of plant cells. dsT-DNA molecules are an important substrate for HR and NHEJ-mediatedGT, yet the mode of their formation from single stranded T-DNA molecules is still obscure. In addition we sought to develop a system for assembly of multi-transgene binary vectors by using ZFNs. The latter may facilitate the production of binary vectors that may be ready for genome editing in transgenic plants. ZFNs were proposed for the induction of DSBs in genomic targets, namely, the FtsH2 gene whose loss of function can easily be identified in somatic tissues as white sectors, and the Cruciferin locus whose targeting by a GFP or RFP reporter vectors can give rise to fluorescent seeds. ZFNs were also proposed for the induction of DSBs in artificial targets and for assembly of multi-gene vectors. We finally sought to address two important cell types in terms of relevance to plant transformation, namely GT of germinal (egg) cells by floral dipping, and GT in somatic cells by root and leave transformation. To be successful, we made use of novel optimized expression cassettes that enable coexpression of all of the genes of interest (ZFNs and Rad genes) in the right tissues (egg or root cells) at the right time, namely when the GT vector is delivered into the cells. Methods were proposed for investigating the complementation of T-strands to dsDNA molecules in living plant cells. During the course of this research, we (i) designed, assembled and tested, in vitro, a pair of new ZFNs capable of targeting the Cruciferin gene, (ii) produced transgenic plants which expresses for ZFN monomers for targeting of the FtsH2 gene. Expression of these enzymes is controlled by constitutive or heat shock induced promoters, (iii) produced a large population of transgenic Arabidopsis lines in which mutated mGUS gene was incorporated into different genomic locations, (iv) designed a system for egg-cell-specific expression of ZFNs and RAD genes and initiate GT experiments, (v) demonstrated that we can achieve NHEJ-mediated gene replacement in plant cells (vi) developed a system for ZFN and homing endonuclease-mediated assembly of multigene plant transformation vectors and (vii) explored the mechanism of dsTDNA formation in plant cells. This work has substantially advanced our understanding of the mechanisms of DNA integration into plants and furthered the development of important new tools for GT in plants.

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Nechushtai, Rachel, and Parag Chitnis. Role of the HSP70 Homologue from Chloroplasts in the Assembly of the Photosynthetic Apparatus. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568743.bard.

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The major goal of the proposed research was to study the role of a 70-kDa heat shock cognate protein from chloroplasts (ct-HSP70) in the assembly of chlorophyll-protein complexes. The latters are mostly important in allowing photosynthesis to occur. Photosynthesis is at the heart of crop productivity and the knowledge of the biogenesis of the photosynthetic apparatus is essential to manipulate the efficiency of photosynthesis. The characterization of the function of the ct-HSP70 was planned to be studied in vitro by assaying its capability to physically interact with the thylakoid proteins and to assist their assembly into thylakoid membranes. We planned to identify regions in the light-harvesting complex protein (LHCP) that interact with the ct-HSP70 and characterize the interaction between them. We also intended to isolate cDNA clones encoding ct-HSP70, sequence them, express one of them in E. coli and use the purified protein for functional assays. The research in this BARD proposal aimed at providing insights and aid in understanding the mechanism by which plants may respond to the heat stress. Since plants often experience increased temperatures.

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Sherman, A., D. N. Kuhn, Y. Cohen, R. Ophir, and R. Goenaga. Exploring the polyembryonic seed trait in mango as a basis for a biotechnology platform for fruit tree crops. United States-Israel Binational Agricultural Research and Development Fund, 2021. http://dx.doi.org/10.32747/2021.8134176.bard.

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Mango is one of the most important fruit crops. However, the biology of this fruit tree is under studied. The lack of genetic and genomic resources has limited progress in mango research and breeding. Several research groups have recently started developing genomic tools for mango by creating transcriptome and genomic data. Sexual reproduction in plants is the main pathway for the creation of new genetic combinations. In modern agriculture, breeders exploit the genetic diversity generated through sexual reproduction to develop elite cultivars; however, these cultivars require genetic stabilization before they are suitable for mass propagation for uniform crop production. In heterozygous plants such as fruit trees, vegetative propagation (cloning) is the primary path for the propagation of genetically uniform plants. Another natural plant mechanism that can create genetically uniform plants (clones) is apomixes. Apomixis is defined as asexual reproduction through seeds that lead to the production of clonal progeny whose genotype is identical to that of the mother plant. In fruit crops like citrus and mango, sporophytic apomixes result in polyembryony, where seeds contain multiple embryos, one of which is sexually originated, and the others are clones of the mother tree. As part of this research, the reference genome of mango was established as a basic platform for mango breeding and research. It was used to map two important mango traits fruit size and polyembryony. The draft genome 'Tommy Atkins' sequence was generated using NRGene de-novo Magic on high molecular weight DNA of 'Tommy Atkins,' supplemented by 10X Genomics long read sequencing to improve the initial assembly. The final 'Tommy Atkins' genome assembly was a consensus sequence that included 20 pseudomolecules representing the 20 chromosomes of mango. The availability of a genome enables the genetic dissection of important traits. We demonstrated the utility of the genome assembly and the 'Tommy Atkins' x 'Kensington Pride' map by analyzing fruit weight phenotypic data and identifying two QTLs for this trait.

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Barkan, Alice, and Zach Adam. The Role of Proteases in Regulating Gene Expression and Assembly Processes in the Chloroplast. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7695852.bard.

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Chloroplasts house many biochemical processes that are essential for plant viability. Foremost, among these is photosynthesis, which requires the protein-rich thylakoid membrane system. The activation of chloroplast genes encoding thylakoid membrane proteins and the targeting and assembly of these proteins together with their nuclear-encoded partners are essential for the elaboration of the thylakoid membrane. Several nuclear-encoded proteins that regulate chloroplast gene expression and that mediate the targeting of proteins to the thylakoid membrane have been identified in recent years, and many more remain to be discovered. The abundance of such proteins is critical and is likely to be determined to a significant extent by their stability, which in turn, is influenced by chloroplast protease activities. The primary goal of this project was to link specific proteases to specific substrates, and in particular, to specific regulatory and assembly proteins. We proposed a two-pronged approach, involving genetic analysis of the consequences of the mutational loss of chloroplast proteases, and biochemical analysis of the degradation pathways of specific proteins that have been shown to control chloroplast gene expression. Our initial bioinformatic analysis of chloroplast proteases allowed us to identify the set of pro teases that is targeted to the chloroplast. We used that information to recover three Arabidopsis mutants with T - DNA insertions in specific chloroplast protease genes. We carried out the first analysis of the stability of a regulator of chloroplast gene expression (CRS2), and found that the protein is much less stable than are typical components of the photosynthetic apparatus. Genetic reagents and analytical methods were developed that have set the stage for a rapid advancement of our understanding of chloroplast proteolysis. The results obtained may be useful for manipulating the expression of transgenes in the chloroplast and for engineering plants whose photosynthetic activity is optimized under harsh environmental conditions.

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