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Artigos de revistas sobre o assunto "3D culture model"
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Liu, Qingxi, Zijiang Zhang, Yupeng Liu, Zhanfeng Cui, Tongcun Zhang, Zhaohui Li e Wenjian Ma. "Cancer cells growing on perfused 3D collagen model produced higher reactive oxygen species level and were more resistant to cisplatin compared to the 2D model". Journal of Applied Biomaterials & Functional Materials 16, n.º 3 (2 de abril de 2018): 144–50. http://dx.doi.org/10.1177/2280800018764763.
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Introduction: Three-dimensional (3D) collagen scaffold models, due to their ability to mimic the tissue and organ structure in vivo, have received increasing interest in drug discovery and toxicity evaluation. Methods: In this study, we developed a perfused 3D model and studied cellular response to cytotoxic drugs in comparison with traditional 2D cell cultures as evaluated by cancer drug cisplatin. Results: Cancer cells grown in perfused 3D environments showed increased levels of reactive oxygen species (ROS) production compared to the 2D culture. As determined by growth analysis, cells in the 3D culture, after forming a spheroid, were more resistant to the cancer drug cisplatin compared to that of the 2D cell culture. In addition, 3D culturing cells showed elevated level of ROS, indicating a physiological change or the formation of a microenvironment that resembles tumor cells in vivo. Conclusions: These data revealed that cellular response to drugs for cells growing in 3D environments are dramatically different from that of 2D cultured cells. Thus, the perfused 3D collagen scaffold model we report here might be a potentially very useful tool for drug analysis.
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Chae, Dong-Sik, Sang Joon An, Seongho Han e Sung-Whan Kim. "Synergistic Therapeutic Potential of Dual 3D Mesenchymal Stem Cell Therapy in an Ischemic Hind Limb Mouse Model". International Journal of Molecular Sciences 24, n.º 19 (27 de setembro de 2023): 14620. http://dx.doi.org/10.3390/ijms241914620.
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Three-dimensional (3D) culture systems have been widely used to promote the viability and metabolic activity of mesenchymal stem cells (MSCs). The aim of this study was to explore the synergistic benefits of using dual 3D MSC culture systems to promote vascular regeneration and enhance therapeutic potential. We used various experimental assays, including dual 3D cultures of human adipose MSCs (hASCs), quantitative reverse transcription polymerase chain reaction (qRT-PCR), in vitro cell migration, Matrigel tube network formation, Matrigel plug assay, therapeutic assays using an ischemic hind limb mouse model, and immunohistochemical analysis. Our qRT-PCR results revealed that fibroblast growth factor 2 (FGF-2), granulocyte chemotactic protein-2 (GCP-2), and vascular endothelial growth factor-A (VEGF-A) were highly upregulated in conventional 3D-cultured hASCs (ASC-3D) than in two-dimensional (2D)-cultured hASCs. Hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF-1), and stromal-cell-derived factor-1 (SDF-1) showed higher expression levels in cytokine-cocktail-based, 3D-cultured hASCs (ASC-3Dc). A conditioned medium (CM) mixture of dual 3D ASCs (D-3D; ASC-3D + ASC-3Dc) resulted in higher migration and Matrigel tube formation than the CM of single 3D ASCs (S-3D; ASC-3D). Matrigel plugs containing D-3D contained more red blood cells than those containing S-3D. D-3D transplantation into ischemic mouse hind limbs prevented limb loss and augmented blood perfusion when compared to S-3D transplantation. Transplanted D-3D also revealed a high capillary density and angiogenic cytokine levels and transdifferentiated into endothelial-like cells in the hind limb muscle. These findings highlight the benefits of using the dual 3D culture system to optimize stem-cell-based therapeutic strategies, thereby advancing the therapeutic strategy for ischemic vascular disease and tissue regeneration.
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Silva, Emmanuel João Nogueira Leal, Nancy Kudsi de Carvalho, Carina Taboada Ronconi, Gustavo De-Deus, Mario Luis Zuolo e Alexandre Augusto Zaia. "Cytotoxicity Profile of Endodontic Sealers Provided by 3D Cell Culture Experimental Model". Brazilian Dental Journal 27, n.º 6 (dezembro de 2016): 652–56. http://dx.doi.org/10.1590/0103-6440201600792.
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Abstract The aim of the present study was to evaluate the cytotoxic effects of five endodontic sealers (AH Plus, Endomethasone N, EndoSequence BC, MTA Fillapex and Pulp Canal Sealer EWT) using a three-dimensional (3D) cell culture model. A conventional bi-dimensional (2D) cell culture model was used as reference technique for comparison. Balb/c 3T3 fibroblasts were cultured in conventional bi-dimensional cell culture and in rat-tail collagen type I three-dimensional cell culture models. Then, both cell cultures were incubated with elutes of freshly mixed endodontic sealers for 24 h. Cell viability was measured by the methyl-thiazol-diphenyltetrazolium assay (MTT). Data were statistically analyzed using ANOVA and the Tukey test at a significance level of p<0.05. All tested sealers exhibited cytotoxic effects; however, cytotoxic effect was culture model- and sealer-dependent. Sealers showed higher cytotoxicity in 2D than in 3D cell culture model (p<0.05). In both conditions, EndoSequence BC showed the lowest cytotoxicity (p<0.05). MTA Fillapex was much more cytotoxic than the other tested endodontic sealers (p<0.05), with the exception of AH Plus in the 2D cell culture model (p>0.05). Endomethasone N and Pulp Canal Sealer EWT showed lower cytotoxic effects than AH Plus in 2D cell culture model (p<0.05); however no statistical differences was observed among these sealers in 3D cell culture model. It may be concluded that cytotoxicity was higher in 2D cell culture compared to 3D cell culture. EndoSequence BC sealer exhibited the highest cytocompatibility and MTA Fillapex the lowest cytocompatibility.
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Kreß, Sebastian, Roland Schaller-Ammann, Jürgen Feiel, Joachim Wegener, Joachim Priedl, Wolf Dietrich, Cornelia Kasper e Dominik Egger. "Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures". Cells 11, n.º 3 (25 de janeiro de 2022): 412. http://dx.doi.org/10.3390/cells11030412.
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The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.
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Rosendahl, Jennifer, Andreas Svanström, Mattias Berglin, Sarunas Petronis, Yalda Bogestål, Patrik Stenlund, Simon Standoft et al. "3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System". Bioengineering 8, n.º 7 (10 de julho de 2021): 97. http://dx.doi.org/10.3390/bioengineering8070097.
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Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker CD44 and the migration markers VIM and SNAI1 in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker CD44 and migration marker VIM which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.
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Bauer, Magdalena, Magdalena Metzger, Marvin Corea, Barbara Schädl, Johannes Grillari e Peter Dungel. "Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture". Life 12, n.º 8 (10 de agosto de 2022): 1216. http://dx.doi.org/10.3390/life12081216.
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In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-aided design model was realized by extrusion-based 3D printing of polylactic acid filaments (PLA). Improvements in the design of the inserts for easier and more efficient handling were confirmed in cell culture experiments. Cytotoxic effects of the final product were excluded by testing the inserts in accordance with ISO-norm procedures. The final versions of the inserts were tested to generate skin-like 3D scaffolds cultured at an air–liquid interface. Stratification of the epidermal component was demonstrated by histological analyses. In conclusion, here we demonstrate a fast and cost-effective method for 3D-printed inserts suitable for the generation of 3D cell cultures. The system can be set-up with common 3D printers and allows high flexibility for generating customer-tailored cell culture plastics.
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Takahashi, Yuki, Yumi Nomura, Yuma Yokokawa, Shiro Kitano, Satoshi Nagayama, Eiji Shinozaki, Ryohei Katayama e Naoya Fujita. "Abstract 4565: Drug screening by layered 3D co-cultured tumor model including vascularized stromal tissue". Cancer Research 83, n.º 7_Supplement (4 de abril de 2023): 4565. http://dx.doi.org/10.1158/1538-7445.am2023-4565.
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Abstract Introduction: In vivo, tumor microenvironments consist of not only cancer cells but also extracellular matrix and stromal tissues, such as fibroblasts, blood vessels, and so on. The interactions between cancer cells and stromal tissue have been reported to affect the behavior of cancer cells. So that ex vivo model recapturing the tumor microenvironment is needed to evaluate the efficacy of drugs under the condition mimicking the patient tumor tissue. Here, we developed the unique tissue engineering technique, which easily enables the construction of cell - stacked three dimensional (3D) tissue, and co-culture of 3D stromal tissues and patient-derived cancer cells (PDCs). We investigated drug sensitivity in conventional 2D culture, our 3D co-cultured model and in vivo tumor. Methods: Fibroblasts and vascular endothelial cells were suspended in a buffer solution containing heparin and collagen to support cell aggregation. The heparin/collagen-treated cells were seeded in culture-inserts in over-confluent manner, and 3D layered stromal tissue called were constructed. PDCs established from colorectal cancer (CRC) and non-small cell lung cancer (NSCLC) patients in the Cancer Institute Hospital of JFCR were co-cultured with the 3D stromal tissue. The 3D co-cultured model applied to drug screening, and the results were compared with those of 2D culture model. In vivo drug evaluations were performed with the compounds in which marked differences were observed between 2D and 3D models. Results: In our 3D model, drug sensitivities to most of the tested compounds tended to be decreased in comparison with those in 2D culture condition. Interestingly, a part of drugs did not effective in 2D showed marked tumor growth inhibition in our 3D model. The compounds that showed favorable efficacy in 3D rather than 2D in multiple PDCs were accounted for about 5% of tested compounds. At least half of these drugs showed significant tumor growth suppression or tumor regression in vivo. On the contrary, in the case of drug sensitivities were considerably fallen in our 3D model, most of the evaluated compounds represented almost no anti-tumor effect in vivo. Results from gene and protein expression analyses supported that cancer cells co-cultured in our 3D stromal tissue have some similar profiles to in vivo tumor rather than 2D culture condition. Conclusion: Our study proposed the unique 3D co-cultured tumor model. The model may enable more accurate drug screening reflecting the in vivo circumstances. Further studies are needed to confirm the model’s predictability of clinical outcomes. Citation Format: Yuki Takahashi, Yumi Nomura, Yuma Yokokawa, Shiro Kitano, Satoshi Nagayama, Eiji Shinozaki, Ryohei Katayama, Naoya Fujita. Drug screening by layered 3D co-cultured tumor model including vascularized stromal tissue. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4565.
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Scalise, Mariangela, Fabiola Marino, Luca Salerno, Nunzia Amato, Claudia Quercia, Chiara Siracusa, Andrea Filardo et al. "Adult Multipotent Cardiac Progenitor-Derived Spheroids: A Reproducible Model of In Vitro Cardiomyocyte Commitment and Specification". Cells 12, n.º 13 (5 de julho de 2023): 1793. http://dx.doi.org/10.3390/cells12131793.
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Background: Three-dimensional cell culture systems hold great promise for bridging the gap between in vitro cell-based model systems and small animal models to study tissue biology and disease. Among 3D cell culture systems, stem-cell-derived spheroids have attracted significant interest as a strategy to better mimic in vivo conditions. Cardiac stem cell/progenitor (CSC)-derived spheroids (CSs) provide a relevant platform for cardiac regeneration. Methods: We compared three different cell culture scaffold-free systems, (i) ultra-low attachment plates, (ii) hanging drops (both requiring a 2D/3D switch), and (iii) agarose micro-molds (entirely 3D), for CSC-derived CS formation and their cardiomyocyte commitment in vitro. Results: The switch from a 2D to a 3D culture microenvironment per se guides cell plasticity and myogenic differentiation within CS and is necessary for robust cardiomyocyte differentiation. On the contrary, 2D monolayer CSC cultures show a significant reduced cardiomyocyte differentiation potential compared to 3D CS culture. Forced aggregation into spheroids using hanging drop improves CS myogenic differentiation when compared to ultra-low attachment plates. Performing CS formation and myogenic differentiation exclusively in 3D culture using agarose micro-molds maximizes the cardiomyocyte yield. Conclusions: A 3D culture system instructs CS myogenic differentiation, thus representing a valid model that can be used to study adult cardiac regenerative biology.
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Metelmann, Isabella B., Sebastian Kraemer, Matthias Steinert, Stefan Langer, Peggy Stock e Olga Kurow. "Novel 3D organotypic co-culture model of pleura". PLOS ONE 17, n.º 12 (1 de dezembro de 2022): e0276978. http://dx.doi.org/10.1371/journal.pone.0276978.
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Pleural mesothelial cells are the predominant cell type in the pleural cavity, but their role in the pathogenesis of pleural diseases needs to be further elucidated. 3D organotypic models are an encouraging approach for an in vivo understanding of molecular disease development. The aim of the present study was to develop a 3D organotypic model of the pleural mesothelium. Specimens of human pleura parietalis were obtained from patients undergoing surgery at the University Hospital Leipzig, Germany. 3D co-culture model of pleura was established from human pleural mesothelial cells and fibroblasts. The model was compared to human pleura tissue by phase-contrast and light microscopy, immunochemistry and -fluorescence as well as solute permeation test. Histological assessment of the 3D co-culture model displayed the presence of both cell types mimicking the morphology of the human pleura. Vimentin and Cytokeratin, PHD1 showed a similar expression pattern in pleural biopsies and 3D model. Expression of Ki-67 indicates the presence of proliferating cells. Tight junctional marker ZO-1 was found localized at contact zones between mesothelial cells. Each of these markers were expressed in both the 3D co-culture model and human biopsies. Permeability of 3D organotypic co-culture model of pleura was found to be higher for 70 kDa-Dextran and no significant difference was seen in the permeability for small dextran (4 kDa). In summary, the presented 3D organoid of pleura functions as a robust assay for pleural research serving as a precise reproduction of the in vivo morphology and microenvironment.
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Prosser, Amy, Colin Scotchford, George Roberts, David Grant e Virginie Sottile. "Integrated Multi-Assay Culture Model for Stem Cell Chondrogenic Differentiation". International Journal of Molecular Sciences 20, n.º 4 (22 de fevereiro de 2019): 951. http://dx.doi.org/10.3390/ijms20040951.
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Recent osteochondral repair strategies highlight the promise of mesenchymal progenitors, an accessible stem cell source with osteogenic and chondrogenic potential, used in conjunction with biomaterials for tissue engineering. For this, regenerative medicine approaches require robust models to ensure selected cell populations can generate the desired cell type in a reproducible and measurable manner. Techniques for in vitro chondrogenic differentiation are well-established but largely qualitative, relying on sample staining and imaging. To facilitate the in vitro screening of pro-chondrogenic treatments, a 3D micropellet culture combined with three quantitative GAG assays has been developed, with a fourth parallel assay measuring sample content to enable normalisation. The effect of transforming growth factor beta (TGF-β) used to validate this culture format produced a measurable increase in proteoglycan production in the parallel assays, in both 2D and 3D culture configurations. When compared to traditional micropellets, the monolayer format appeared less able to detect changes in cell differentiation, however in-well 3D cultures displayed a significant differential response. Effects on collagen 2 expression confirmed these observations. Based on these results, a microplate format was optimised for 3D culture, in a high-throughput in-well configuration. This model showed improved sensitivity and confirmed the 3D micropellet in-well quantitative assays as an effective differentiation format compatible with streamlined, high-throughput chondrogenic screens.
Teses / dissertações sobre o assunto "3D culture model"
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Zhao, Huizhi. "3D Cell Culture Model Synthesized By Polycaprolactone Nanofiber Electrospinning". Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1531319675295094.
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Peddagangannagari, Sreekanth Reddy. "An in vitro human 3D co-culture model to study endothelial-astrocyte interactions". Thesis, Open University, 2012. http://oro.open.ac.uk/54831/.
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At the gliovascular interface, reciprocal inductive influences between brain microvascular endothelial cells (BMVEC) and astrocytes occur. Most of the knowledge in this area of research is derived from in vitro eo-culture models in which astrocytes are cultured on a stiff, two-dimensional (2D) surface. Three-dimensional (3D) culture models closely mimic the in lJZVO cellular architecture and they bridge the gap between 2D culture models and animal models. Hence, an in vitro 3D eo-culture model was developed and characterised, to study the interactions between BMVEC and astrocytes. In this model, human astrocytes (HA) were seeded inside a collagen type--I gel while human immortalised cerebral microvascular endothelial cells (hCMEC/D3) were cultured on the gel surface. Both cell types were of human origin to improve the translatability of findings to humans in vivo. Additional important features of the model are the culture of endothelial cells on a soft matrix, and the simulation of the geometric relationship that exists in vivo i.e., the interaction of astrocytes with BMVEC from their ab luminal side. To determine the effect of the 3D environment on the HA, the proliferation rate and expression of four molecules namely, glial fibrillary acidic protein (GF AP), aquaporin-4 (AQP4), endothelin-l (Et-l) and endothelin receptor type-B (EDNRB), were compared between 3D and 2D cultured HA. The decreased expression of AQP4 and EDNRB and the much-decreased proliferation rate of 3D HA suggested their reduced reactivity and a similarity to their in lJiVO counterparts. However, 3D HA did not differ from the 2D HA in their ability to release soluble factors that induce barrier properties on BMVEC, as observed by similar levels of three expression markers of barrier phenotype on hCMEC/D3 cells namely, zonula occludens-l, claudin-5, and P-glycoprotein and similar paracellular permeability coefficients to fluorescent-dextrans (70 kDa).
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Smith, Jenny Thompson. "A 3D culture model to investigate cellular responses to mechanical loading in spinal cord injury". Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/16199/.
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Spinal cord injury (SCI) can cause paralysis, loss of sensation, and respiratory dependency, which has a significant impact on the quality of life of patients, their life expectancy and is also a significant economic burden due to the high costs associated with primary care and loss of income. One of the difficulties in establishing a treatment method is the heterogeneity of SCI; there are many different types and severities of traumatic primary injury, across different age groups of patients and different locations within the spinal cord, whilst at a cellular level, there are multiple, interacting secondary injury cascades that amplify the primary damage inflicted during the traumatic insult. Many techniques have been developed to mimic particular injuries found in human SCI, however in vivo animal models can be extremely costly and time consuming. The modest translation of therapeutic treatments from animal models to successful clinical trials suggests that there is a need for simplified models of SCI, in which the complex secondary cascade can be broken down into specific cellular interactions under controlled injury parameters. It was hypothesised that in vivo injuries could be simulated using a 3D in vitro model of SCI within a tethered, self-aligned, type-I collagen gel. An in vitro model such as this could advance the understanding of cellular responses to injury and help inform animal studies which may facilitate the design of therapeutics. Initially, different matrices were investigated in order to determine their suitability for use as matrix components for a 3D in vitro model of SCI. The matrices were characterised in terms of their mechanical properties, and the cellular responses of astrocytes following culture within the matrices. A fully hydrated matrix was selected which had a lower elastic modulus in comparison to spinal cord tissue, and which maintained astrocytes in a non-reactive state, as determined by the expression of markers for reactive astrogliosis. Contusion models of SCI are thought to generate the most relevant animal models of SCI, therefore their suitability as an injury mechanism within a 3D cellular model was investigated. A pilot study using the Hatteras contusion device, demonstrated that there was potential for in vivo type contusion devices to be utilised with an in vitro 3D collagen gel SCI model. The remainder of the study utilised the Infinite Horizons (IH) in vivo impactor, which is a force controlled contusion device. The experimental parameters utilised with the IH impactor within an in vivo setting were investigated as to their suitability for collagen gel impactions. Following a detailed investigation, the in vivo parameters of an impact force of 200 kdyn and a dwell time of 0 ms, using a 2.5 mm diameter impaction tip were adopted; however the calibration start height of the impaction tip was altered to avoid full penetration of the impactor tip through the gel. The limitations of the contusion device affected the consistency of the impaction and resulted in a lack force output data. These limitations need to be resolved in order to directly compare in vivo and in vitro SCI using the IH impactor. The impaction of 3D aligned, collagen gels, seeded with primary rat astrocytes, using the IH impactor generated a 3D cavity bordered by reactive astrocytes, which was reminiscent of the glial scar and cystic cavity which forms at the lesion site in vivo. An increasing gradient of the astrocyte reactivity marker, glial fibrillary acidic protein, was expressed by cells closest to the impact zone. Astrocytes within the first 100 µm of the impact zone were highly ramified with cellular filaments aligned with the edge of the impact zone. An increase in the expression of astrocyte reactivity markers was observed over a ten-day period following impaction. In summary, a 3D model of SCI was developed that was highly adaptable, and suitable for further advancement to increase the complexity and experimental outputs that were presented in this study. More detailed analysis of the cellular responses, over longer time courses, and perhaps with the additional complexity of multiple cell types would complement investigations within in vivo models. 3D in vitro tethered collagen gel models such as this could provide valuable insights into the cellular mechanisms which may progress the translation of treatments into the clinic.
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Manzan, Martins Camilla. "EFFECT OF ENDOCRINE DISRUPTORS ON HUMAN ENDOMETRIAL STROMAL CELLS AND THEIR INTERACTION WITH TROPHOBLAST". Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1183943.
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Decidualization is crucial for embryo development and implantation, placenta formation and fetal growth. This process is characterized by morphological and biological changes in endometrial stromal cells that play a key role on fetal trophoblast migration and invasion. Successful placentation depends on the interaction between endometrial stromal cells and extravillous trophoblast cells. The trophoblast spheroids, a 3D culture model, is reported to appropriately mimic the in vivo situation, and reflect the cell to cell interaction. The Bisphenol A (BPA) and para-nonylphenol (p-NP) are endocrine disrupting chemicals (EDCs), present in the polycarbonate plastics used in many products such as food packaging, bottles and beverage cans and as an intermediate in the production of phenolic resins. Studies demonstrated that maternal exposure to EDCs, at environmentally relevant concentrations, are associated to aberrant early embryo development and uterine receptivity due to their estrogenic activity. Nowadays it is known that environmental contaminants can change stromal cell decidualization and trophoblast migration. In the present study, we developed a simple 3D culture model using transformed human endometrial stromal cells (tHESCs) and immortalized first trimester human extravillous trophoblast cells (HTR-8/SVneo). The aim of this work was to evaluate the effect of BPA and p-NP on endometrial stromal cells during decidualization and their interaction with trophoblast spheroids. The data showed that pre-exposition to p-NP of endometrial stromal cells impaired decidualization interfering on the cross-talk with trophoblast and altering lysosomes biogenesis and consequently leading to an impairment in trophoblast migration.
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Sieh, Shirly. "Development of a 3D culture system to study the skeletal metastasis of prostate cancer". Thesis, Queensland University of Technology, 2011. https://eprints.qut.edu.au/50870/1/Shirly_Sieh_Thesis.pdf.
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In the cancer research field, most in vitro studies still rely on two-dimensional (2D) cultures. However, the trend is rapidly shifting towards using a three-dimensional (3D) culture system. This is because 3D models better recapitulate the microenvironment of cells, and therefore, yield cellular and molecular responses that more accurately describe the pathophysiology of cancer. By adopting technology platforms established by the tissue engineering discipline, it is now possible to grow cancer cells in extracellular matrix (ECM)-like environments and dictate the biophysical and biochemical properties of the matrix. In addition, 3D models can be modified to recapitulate different stages of cancer progression for instance from the initial development of tumor to metastasis. Inevitably, to recapitulate a heterotypic condition, comprising more than one cell type, it requires a more complex 3D model. To date, 3D models that are available for studying the prostate cancer (CaP)-bone interactions are still lacking. Therefore, the aim of this study is to establish a co-culture model that allows investigation of direct and indirect CaP-bone interactions. Prior to that, 3D polyethylene glycol (PEG)-based hydrogel cultures for CaP cells were first developed and growth conditions were optimised. Characterization of the 3D hydrogel cultures show that LNCaP cells form a multicellular mass that resembles avascular tumor. In comparison to 2D cultures, besides the difference in cell morphology, the response of LNCaP cells to the androgen analogue (R1881) stimulation is different compared to the cells in 2D cultures. This discrepancy between 2D and 3D cultures is likely associated with the cell-cell contact, density and ligand-receptor interactions. Following the 3D monoculture study, a 3D direct co-culture model of CaP cells and the human tissue engineered bone (hTEBC) construct was developed. Interactions between the CaP cells and human osteoblasts (hOBs) resulted in elevation of Matrix Metalloproteinase 9 (MMP9) for PC-3 cells and Prostate Specific Antigen (PSA) for LNCaP cells. To further investigate the paracrine interaction of CaP cells and (hOBs), a 3D indirect co-culture model was developed, where LNCaP cells embedded within PEG hydrogels were co-cultured with hTEBC. It was found that the cellular changes observed reflect the early event of CaP colonizing the bone site. In the absence of androgens, interestingly, up-regulation of PSA and other kallikreins is also detected in the co-culture compared to the LNCaP monoculture. This non androgenic stimulation could be triggered by the soluble factors secreted by the hOB such as Interleukin-6. There are also decrease in alkaline phosphatase (ALP) activity and down-regulation of genes of the hOB when co-cultured with LNCaP cells that have not been previously described. These genes include transforming growth factor β1 (TGFβ1), osteocalcin and Vimentin. However, no changes to epithelial markers (e.g E-cadherin, Cytokeratin 8) were observed in both cell types from the co-culture. Some of these intriguing changes observed in the co-cultures that had not been previously described have enriched the basic knowledge of the CaP cell-bone interaction. From this study, we have shown evidence of the feasibility and versatility of our established 3D models. These models can be adapted to test various hypotheses for studies pertaining to underlying mechanisms of bone metastasis and could provide a vehicle for anticancer drug screening purposes in the future.
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Vazquez, Marisol. "Development of a novel in vitro 3D osteocyte-osteoblast co-culture model to investigate mechanically-induced signalling". Thesis, Cardiff University, 2013. http://orca.cf.ac.uk/56764/.
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Normal mechanical loading potently induces bone formation mediated by osteocyte effects on osteoblasts. Current in vitro bone models do not reflect these cellular interactions, either focusing on mechanical loading of osteoblasts in monolayers or in 3D and therefore not elucidating the osteocyte-osteoblast interactions that regulate mechanically-induced bone formation. Adenosine, calcium-sensing and glutamate signalling have been shown to influence bone biology, with both adenosine precursors and glutamate having been implicated in mechanotransduction. The aims were to develop a novel in vitro 3D co-culture model of bone to investigate mechanically-induced signalling, and to determine the expression of adenosine, calcium-sensing and glutamate signalling components within the 3D model and their contribution to the regulation of mechanically-induced bone formation markers. A 3D model was developed as a two-phase culture system where MLO-Y4 osteocytes were embedded within type I collagen gels and MC3T3-E1osteoblasts were layered on top. In this model, cells were viable over 7 days (100 % osteoblasts, 87 % osteocytes), maintained appropriate morphology and contacted neighbouring cells through CX43 labelled projections. RT-qPCR revealed Runx2, OCN and E11 mRNA expression in both osteoblasts and osteocytes. COL1A1 mRNA expression was significantly higher in the osteoblasts (P=0.0001), whereas ALP mRNA was higher in the osteocytes (P=0.001). RT-PCR revealed expression of adenosine receptors A2A and A2B and glutamate transporter GLAST1 in osteoblasts and osteocytes, as well as glutamate receptors AMPAR2 and KA1 in osteocytes. Immunostaining confirmed expression of A2A, GLAST1 and KA1, and revealed expression of CaSR, in both osteoblasts and osteocytes. A novel mechanical loading device was developed which was used to apply osteogenic loads (5 min, 10 Hz, 2.5 N) to 3D osteocyte mono-cultures and 3D osteocyte-osteoblast co-cultures. A minimum of 48 hr pre-load time was required for a reliable load response. 3D osteocyte mono-cultures cultured for 48-72 hr or 7 days pre-load, remained viable, significantly increased PGE2 0.5 hr after load (48-72 hr: P=0.0249, 7 days: P=0.041) and decreased their IL-6 synthesis. RT-qPCR revealed a load-induced decrease in E11 (P=0.018) and RANKL (P=0.0486) mRNA, in 48-72 hr cultures. In 7 day cultures, E11 mRNA (P=0.041) increased as a result of loading. Preliminary data showed that the same loading conditions increased PINP synthesis, a bone formation marker, in 3D co-cultures (P=0.022). The AMPA/KA receptors antagonist NBQX increased PINP synthesis by 2-fold over 5 days, similar levels induced by loading in untreated cultures, suggesting that NBQX has similar anabolic effects as mechanical stimuli. Similarly, the A2A receptor antagonist SCH 442416 increased osteoblast ALP mRNA expression by 3.5-fold at day 1 post-load and increased PINP synthesis by 1.9-fold, in co-cultures after 5 days. This 3D osteocyte-osteoblast co-culture model represents a useful in vitro model for the investigation of the osteocyte-osteoblast interactions that lead to mechanically-induced signalling and regulation of bone formation markers. Adenosine, calcium-sensing and glutamate signalling components are expressed within the model, facilitating future investigations of their roles in mechanically-induced signalling. Preliminary experiments indicated that adenosine and glutamate signalling may each contribute individually to the regulation of mechanically-induced bone formation markers.
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Smolina, Margarita. "Breast cancer cell lines grown in a three-dimensional culture model: a step towards tissue-like phenotypes as assessed by FTIR imaging". Doctoral thesis, Universite Libre de Bruxelles, 2018. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/267686.
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Despite the possible common histopathological features at diagnosis, cancer cells present within breast carcinomas are highly heterogeneous in their molecular signatures. This heterogeneity is responsible for disparate clinical behaviors, treatment responses and long-term outcomes in breast cancer patients. Although the few histopathological markers can partially describe the diversity of cells found in tumor tissue sections, the full molecular characterization of individual cancer cells is currently impossible in routine clinical practice. In this respect, Fourier transform infrared (FTIR) microspectroscopic imaging of histological sections allows obtaining, for each pixel of tissue images, hundreds of independent potential markers, which makes this technique a particularly powerful tool to distinguish cell types and subtypes. As a complement to the conventional clinicopathological evaluation, this spectroscopic approach has the potential to directly reveal molecular descriptors that should allow identifying different clonal lineages found within a single tumor and therefore provide knowledge relevant to diagnosis, prognosis and treatment personalization. Yet, interpretation of infrared (IR) spectra acquired on tissue sections requires a well-established calibration, which is currently missing. Conventionally, mammary epithelial cells are studied in vitro as adherent two-dimensional (2D) monolayers, which lead to the alteration of cell-microenvironmental interplay and consequently to the loss of tissue structure and function. A number of key in vivo-like interactions may be re-established with the use of three-dimensional (3D) laminin-rich extracellular matrix (lrECM)-based culture systems. The aim of this thesis is to investigate by FTIR imaging the influence of the in vitro growth environment (2D culture versus 3D lrECM culture and 3D monoculture versus 3D co-culture with fibroblasts) on a series of thirteen well-characterized human breast cancer cell lines and to determine culture conditions generating spectral phenotypes that are closer to the ones observed in malignant breast tissues. The reference cell lines cultured in a physiologically relevant basement membrane model and having undergone formalin fixation, paraffin embedding (FFPE), a routine treatment used to preserve clinical tissue specimens, could contribute to the construction of a spectral database. The latter could be ultimately employed as a valuable tool to interpret IR spectra of cells present in tumor tissue sections, particularly through the recognition of unique spectral markers.To achieve the goal, we developed and optimized, in a first step, the preparation of samples derived from traditional 2D and 3D lrECM cell cultures in order to preserve their morphological and molecular relevance for FTIR microspectroscopic analysis. We then highlighted the importance of the influence of the growth environment on the cellular phenotype by comparing spectra of 2D- and 3D-cultured breast cancer cell lines between them. A particular focus was placed to establish a correlation between FTIR spectral data and publicly available microarray-based gene expression patterns of the whole series of breast cancer cell lines grown in 2D and 3D lrECM cultures. Our results revealed that, although based on completely different principles, gene expression profiling and FTIR spectroscopy are similarly sensitive to both the cell line identity and the phenotypes induced by cell culture conditions. We also identified by FTIR imaging changes in the chemical content occurring in the microenvironment surrounding cell spheroids grown in 3D lrECM culture model. Finally, we illustrated the impact of the in vivo-like microenvironment on the IR spectra of breast cancer cell lines grown in 3D lrECM co-culture with fibroblasts and compared them with spectra of cell lines grown in 3D lrECM monoculture. Unsupervised statistical data analyses reported that cells grown in 3D co-cultures produce spectral phenotypes similar to the ones observed in FFPE tumor tissue sections from breast carcinoma patients. Altogether, our results suggest that FFPE samples prepared from 3D lrECM cultures of breast cancer cell lines and studied by FTIR microspectroscopic imaging provide reliable information that could be integrated in the setting up of a recognition model aiming to identify and interpret specific spectral signatures of cells present in breast tumor tissue sections.Le cancer du sein est une maladie très hétérogène, tant au niveau clinique que biologique. Cette hétérogénéité rend impossible la caractérisation moléculaire complète des cellules cancéreuses individuelles dans la pratique clinique courante. Dans ce contexte, l’imagerie infrarouge à transformée de Fourier (FTIR) des coupes tissulaires permet d'obtenir pour chaque pixel d'une image de tissu des centaines de marqueurs potentiels indépendants, ce qui pourrait faire de cette technique un outil particulièrement puissant pour identifier des différents types et sous-types cellulaires. L'interprétation des spectres infrarouges (IR) enregistrés à partir des coupes histologiques nécessite cependant une calibration qui fait actuellement défaut. Cette calibration pourrait être obtenue à partir de lignées cellulaires tumorales bien caractérisées. Traditionnellement, les cellules épithéliales mammaires sont étudiées in vitro sous forme de monocouches adhérentes bidimensionnelles (2D), ce qui conduit à l'altération de la communication entre les cellules et leur environnement et, par conséquent, à la perte de l’architecture et de la fonction du tissu épithélial. Un certain nombre d'interactions physiologiques clés peuvent être rétablies en utilisant des systèmes de culture tridimensionnelle (3D) dans une matrice extracellulaire riche en laminine (lrECM). L'objectif de cette thèse consiste à étudier par imagerie FTIR l'influence du microenvironnement (via une comparaison entre les cultures 2D et 3D lrECM ou les cultures 3D lrECM en présence ou en l’absence de fibroblastes) sur une série de treize lignées de cellules tumorales mammaires humaines bien caractérisées et à déterminer les conditions de culture générant des phénotypes spectraux qui se rapprochent le plus de ceux observés dans les tissus tumoraux. Au cours de ce travail, nous avons mis au point la culture des lignées cellulaires dans un modèle 3D lrECM ainsi qu’une méthodologie de préparation des échantillons offrant la possibilité de les comparer de manière pertinente avec les cellules cancéreuses présentes dans les coupes histologiques. De même, nous avons étudié par imagerie FTIR les effets du microenvironnement sur les lignées de cellules tumorales et inversement. Pour les lignées investiguées, le passage d’une culture 2D à une culture 3D lrECM s’accompagne, en effet, de modifications du spectre IR étroitement corrélées aux modifications du transcriptome. Les marqueurs spectraux indiquent également que l’environnement 3D génère un phénotype cellulaire proche de celui trouvé dans les coupes histologiques. De manière intéressante, cette proximité est d’autant plus renforcée en présence de fibroblastes dans le milieu de culture.
Doctorat en Sciences agronomiques et ingénierie biologique
info:eu-repo/semantics/nonPublished
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Sorrentino, Rita. "Three dimensional oral mucosa models: development and applications". Doctoral thesis, Università del Piemonte Orientale, 2020. http://hdl.handle.net/11579/114910.
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Animal experimentation has been extensively and for a long time applied in several research fields, but since 2011 it has been substantially limited by the Commission of the European Parliament to ensure people/animals safety and reduce research costs. To respond to these directives, many attempts have been focused on the development and validation of new in vitro 3D systems, bypassing the traditional 2D cell cultures. In this regard, diverse approaches to tissue-engineered bone and oral mucosa have been developed. Despite the promising premises and the cutting-edge results, the used 3D in vitro bone-oral mucosal models still lack interaction between the mucosal and the bone components. Therefore, this project aimed to create 3D models, entirely made with primary human cells (keratinocytes, fibroblasts, and osteoblasts), able to mimic the natural structure and interaction of bone and oral mucosa. In the present work, the regulatory role of the mesenchymal tissue onto epithelia was evaluated. The main results showed that that during the differentiation hMSC produce and secrete factors that induce the keratinization and the expression of the marker of differentiation CK10; in particular in the
middle stage of differentiation (OB14). The proteomic analysis revealed that this effect can be ascribable to KGF
secretion. This finding may impact the design of new implantable devices able to induce, alone, the epithelial
growth and keratinization to improve implant graft avoiding epithelial graft linked to the morbidity of another
zone. Moreover, we also showed that OM might have a pro-innervation effect, at least during the last stages of
keratinocytes stratification. Finally, we obtained and characterized an innervated mucoperiosteal model that could
open new in vitro frontiers for oral biomaterials validation as well as improve knowledge regarding the
mesenchymal stem cells roles onto oral mucosa development.
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Cho, Hyung Joon. "Pro-oxidative and Pro-inflammatory Mechanisms of Brain Injury in Experimental Animal and 3D Cell Culture Model Systems". Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/73476.
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The pro-oxidative and pro-inflammatory mechanisms have been implicated in various human diseases including neurological and psychiatric disorders. However, there is only limited information available on the etiology in the progression of neurological damage to brain. The emergence of tissue engineering with the growing interest in mechanistic studies of brain injury now raises great opportunities to study complex physiological and pathophysiological process in vitro. Therefore, the prime goals of this study include: (1) Determination of the molecular and cellular mechanisms responsible for blast- and radiation-induced brain injuries and (2) Development of a three-dimensional (3D) model system in order to mimic in vivo-like microenvironments to further broaden our knowledge in pro-oxidative and pro-inflammatory mechanisms and their cellular responses within 3D constructs.
In the first study, we demonstrated that blast exposure induced specific molecular and cellular alterations in pro-oxidative and pro-inflammatory environments in the brain and neuronal loss with adverse behavioral outcome. The results provide evidence that pro-oxidative and pro-inflammatory environments in the brain could play a potential role in blast-induced neuronal loss and behavioral deficits.
In the second study, we investigated that fractionated whole-brain irradiation induced specific molecular and cellular alterations in pro-oxidative and pro-inflammatory environments in the brain along with elevation of reactive oxygen species (ROS)-generating protein (NOX-2) and microglial activation. Additionally, the contribution of NOX-2 in fractionated whole-brain radiation-induced oxidative stress was observed by dramatic amelioration of ROS generation after pharmacological inhibition of NOX-2. These results support that NOX-2 may play a pivotal role in fractionated whole-brain radiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain.
In the third study, we developed an in vitro 3D experimental model of brain inflammation by encapsulating microglia in collagen hydrogel with computational analysis of 3D constructs. The results indicated that our newly developed in vitro 3D model system provides a more physiologically relevant environment to mimic in vivo responses.
In conclusion, these data may be beneficial in defining a cellular and molecular basis of pathophysiological mechanisms of brain injuries. Furthermore, it may provide new opportunities for preventive and therapeutic interventions for patients with brain injuries and associated neurological disorders.Ph. D.
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Lee, Si Yuen. "Culture of human pluripotent stem cells and neural networks in 3D using an optogenetic approach and a hydrogel model". Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:5cecda23-6208-4c0f-a800-d5ddccae24d3.
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Development of optogenetically controllable human neural network models can provide an investigative system that is relevant to the human brain. Conventional cultures of neural networks in two-dimensions (2D) have major limitations of scale. For instance, the soma of neurons in 2D is unrealistically flattened and both axon and dendrite outgrowth is restricted. Using a combination of tissue engineering techniques and the inclusion of optogenetically modified human induced pluripotent stem cell (hiPSC)-derived neural progenitor cells (NPCs), the development of a three-dimensional (3D) human neural culture model within a defined 3D microenvironment is investigated in this study. Light-sensitive neurons were successfully generated by transducing Channelrhodopsin-2 (ChR2) into human iPSC-derived NPCs and neuroblastoma cells (SH-SY5Y) using lentiviral transduction. The use of neuron specific promoters for synapsin-1 (SYN1) and calcium-calmodulin kinase II (CaMKII) in driving the expression of ChR2-Yellow Florescent Protein (YFP) within the mixed neuronal populations from hiPSC-derived neurons (Axol cells) were compared. Viability of the cells at 7 day-post-infection was 80% - 97% in all conditions tested. In line with published literature, transduction efficiency of neurons at day 14 was found to be 3% - 7% for plasmids containing the SYN1 promoter and 2% - 5% for plasmids containing the CaMKII promoter. An increase in promoter driven ChR2-YFP expression was evaluated over 28 days as the neural subpopulations matured. Stably ChR2 expression continued through-out higher passages (≥ P10) and possibly for periods up to several months. Both SYN1 and CaMKII promoters were found to drive the expression of ChR2 in Axol cells targeting inhibitory and excitatory neurons, respectively. 3D culture systems to support cell growth and optogenetic application were developed and characterised. Alginate hydrogel functionalised with short peptide sequence arginine-glycine-aspartate (RGD), and small molecules such as Rho Kinase inhibitor (ROCKi) and ZVAD were incorporated to increase the viability of human pluripotent stem cells (hPSCs). Investigation of cell response reveals that a flow rate of 3 ml/min and an alginate concentration of 1.8% (w/v) are optimal and that stem cell survival is significantly improved through incorporation of RGD and ROCKi. Interestingly, ChR2-YFP expression of Axol and SY5Y cells was detectable when transferred to the 3D culture system. The optogenetically modified neurons were found responsive to light stimulation, showing firing patterns and calcium events typical of early developing neurons (e.g. mixed and burst waves; single and multipeak spikes). Neuronal activities were assessed using calcium imaging. Higher numbers of calcium events were associated with CaMKII driven ChR2-YFP expression than with SYN1 in Axol cells. However, calcium activity in SH-SY5Y cells was most noticeable in neurons expressing ChR2-YFP driven by the SYN1 promoter. In primary rodent neuronal cultures, synchronous calcium firing with repetitive action potentials (APs) resulted from ChR2-YFP expression was driven by both SYN1 and CaMKII promoter upon light stimulation. By combining multi-approaches, we report for the first time on the generation of an in vitro hiPSC-derived neural network model in 3D using functionalised alginate hydrogel and involving optogenetic targeting. Expression of ChR2-YFP was found driven by both SYN1 and CaMKII promoter in the RGD-alginate bead system that cultured with Axol cells.
Livros sobre o assunto "3D culture model"
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Dipasquale, Letizia, Saverio Mecca e Mariana Correia, eds. From Vernacular to World Heritage. Florence: Firenze University Press, 2020. http://dx.doi.org/10.36253/978-88-5518-293-5.
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This publication brings together the results of the project 3DPAST: Living and virtual visiting European World Heritage, co-funded by the Creative Europe EU programme. The research highlighted the exceptional character and quality of living in vernacular dwellings found in World Heritage sites. This was possible by seizing the cultural space of European vernacular heritage, located in Pico island (Portugal), Cuenca town (Spain), Pienza (Italy), Old Rauma (Finland), Transylvania (Romania), Berat & Gjirokastra (Albania), Pátmos (Greece), and Upper Svaneti (Georgia). New digital realities grant the possibility to visit and to appreciate those places, to non-travelling audiences, who lack the opportunity to experience this unique heritage in situ. Creative potential is highlighted in 3D models and digital visualisations, which associate outstanding local knowledge with the vernacular expression of World Heritage.
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Balcıoğlu, Tevfik. On Design. Bloomsbury Publishing Plc, 2024. http://dx.doi.org/10.5040/9781350359345.
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This volume presents for the first time a curated selection of essays written over the last 30 years by leading design thinker and educator, Tevfik Balcioglu. With a focus on Turkish and British design, his writing examines questions of national and transnational design history and provides a critical insight into contemporary global design issues. Structured into four thematic sections with contextualizing introductions, this anthology addresses various aspects of design history, theory, education and practice. Essays look at the impact of industrialization and globalization on design cultures and highlight local and global design developments from the late 20th century to the present day. They cover reproduction techniques and technological progress in recent decades, the changing nature of mass-produced objects and the introduction of new methods, systems, shapes, forms and styles over time. Addressing issues relating to education and practice, case studies draw on Balcioglu’s work at various institutions such as Izmir University of Economics, Turkey where he established a faculty of design departments and introduced a new model of integrated programmes, and Kent University, UK where he established the BA (Hons) 3D Design course. His writing explores the nature and transferability of knowledge in the design field through critical analysis of the emergence of new degree programmes, the evolution of design education and the relationship between theory and practice.
Capítulos de livros sobre o assunto "3D culture model"
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Miller, Daniel H., Ethan S. Sokol e Piyush B. Gupta. "3D Primary Culture Model to Study Human Mammary Development". In Methods in Molecular Biology, 139–47. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7021-6_10.
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Chioni, Athina-Myrto, Rabia Tayba Bajwa e Richard Grose. "3D Organotypic Culture Model to Study Components of ERK Signaling". In Methods in Molecular Biology, 255–67. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6424-6_19.
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Shay, Chloe, e Yong Teng. "Evaluating the Activity of Using a Novel 3D Culture Model". In Methods in Molecular Biology, 159–64. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1558-4_9.
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Flint, Lucy. "Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model". In Methods in Molecular Biology, 147–59. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3319-9_13.
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Yan, Yuanwei, e Su-Chun Zhang. "Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture". In Stem Cell-Based Neural Model Systems for Brain Disorders, 1–11. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3287-1_1.
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Augustine, Tanya N. "Analysis of Immune-Tumor Cell Interactions Using a 3D Co-culture Model". In Methods in Molecular Biology, 103–10. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0802-9_8.
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Pendić, Jugoslav, e Barry Molloy. "The Use of 3D Documentation for Investigating Archaeological Artefacts". In The 3 Dimensions of Digitalised Archaeology, 9–26. Cham: Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-53032-6_2.
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AbstractWhile 3D rendering of archaeological features in the field is becoming a standard documentary procedure, in the case of objects it remains less well-integrated as a functional resource, when compared to conventional illustration and photography. This paper examines the current state of the art for 3D data workflows, as used in the study of material culture in archaeology. In doing so, we touch upon the historical-technological background of this mode of documentation and observe its current level of impact on what we may consider normal ways of interacting with archaeological assemblages. We underline how current data-management and production issues diminish potential interoperability across 3D model-making platforms and lead to an escalation in data-storage consumption.
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Spoerri, Loredana, Kimberley A. Beaumont, Andrea Anfosso e Nikolas K. Haass. "Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma". In Methods in Molecular Biology, 401–16. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7021-6_29.
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Fu, Xiangyu, Gengkang Lian e Jing Zhao. "Exploring the Blending of Ancient and Modern Chinese Culture through a 3D Model". In Proceedings of the 2023 International Conference on Data Science, Advanced Algorithm and Intelligent Computing (DAI 2023), 485–97. Dordrecht: Atlantis Press International BV, 2024. http://dx.doi.org/10.2991/978-94-6463-370-2_50.
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Blazquez, Raquel, Daniela Sparrer, Jessica Sonbol, Jürgen Philipp, Florian Schmieder e Tobias Pukrop. "Organotypic 3D Ex Vivo Co-culture Model of the Macro-metastasis/Organ Parenchyma Interface". In Methods in Molecular Biology, 165–76. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3674-9_12.
Texto completo da fonteTrabalhos de conferências sobre o assunto "3D culture model"
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Rodkhwan, Supasasi, e Pizzanu Kanongchaiyos. "Shape Retrieval for Khon 3D Model". In 2013 International Conference on Culture and Computing (Culture Computing). IEEE, 2013. http://dx.doi.org/10.1109/culturecomputing.2013.20.
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Shemeneva, Anastasia Valerievna. "Legal culture as a model of social governance". In 3d International Scientific and Practical Conference. TSNS Interaktiv Plus, 2017. http://dx.doi.org/10.21661/r-115802.
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Chaluvally-Raghavan, P., A. Zeisel, W. Koestler, J. Jacob-Hirsch, G. Rechavi, E. Domany e Y. Yarden. "HER2-Associated Breast Cancer Signature Using a 3D Culture Model." In Abstracts: Thirty-Second Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 10‐13, 2009; San Antonio, TX. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-09-4146.
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Patel, Kalpana, Belinda O'Clair, Tim O'Callaghan, Daniel M. Appledorn e Derek Trezise. "Abstract 4295: A 3D culture model for screening of cancer therapeutics". In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4295.
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Marshall, Lauren, Isabel Löwstedt, Paul Gatenholm e Joel Berry. "Prevascularized, Co-Culture Model for Breast Cancer Drug Development". In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80409.
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The objective of this study was to create 3D engineered tissue models to accelerate identification of safe and efficacious breast cancer drug therapies. It is expected that this platform will dramatically reduce the time and costs associated with development and regulatory approval of anti-cancer therapies, currently a multi-billion dollar endeavor [1]. Existing two-dimensional (2D) in vitro and in vivo animal studies required for identification of effective cancer therapies account for much of the high costs of anti-cancer medications and health insurance premiums borne by patients, many of whom cannot afford it. An emerging paradigm in pharmaceutical drug development is the use of three-dimensional (3D) cell/biomaterial models that will accurately screen novel therapeutic compounds, repurpose existing compounds and terminate ineffective ones. In particular, identification of effective chemotherapies for breast cancer are anticipated to occur more quickly in 3D in vitro models than 2D in vitro environments and in vivo animal models, neither of which accurately mimic natural human tumor environments [2]. Moreover, these 3D models can be multi-cellular and designed with extracellular matrix (ECM) function and mechanical properties similar to that of natural in vivo cancer environments [3].
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Cheluvaraju, Chaitra, Stephen Shuford, Christina Mattingly, Teresa DesRochers, Matthew Gevaert, David E. Orr e Hal E. Crosswell. "Abstract 3935: A perfused 3D co-culture model of vemurafenib-resistant melanoma". In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3935.
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Medearis, S., R. Brown, K. Pollard, A. Bosak, C. Dugas, A. Das, R. Sato, V. Traina-Dorge, M. Moore e G. Piedimonte. "3D Culture Model to Characterize RSV Infection in the Peripheral Nervous System". In American Thoracic Society 2022 International Conference, May 13-18, 2022 - San Francisco, CA. American Thoracic Society, 2022. http://dx.doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a3126.
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Sun, Jinfeng. "The construction of 3D model of safty culture system in CNPC's oil factories". In 2011 International Conference on E-Business and E-Government (ICEE). IEEE, 2011. http://dx.doi.org/10.1109/icebeg.2011.5885320.
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Irigoyen, Macarena, e Gonzalo Castillo. "Abstract 5195: Efficacy of histone deacetylase inhibitors in a 3D cell culture model". In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-5195.
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Irigoyen, Macarena, e Gonzalo Castillo. "Abstract 5195: Efficacy of histone deacetylase inhibitors in a 3D cell culture model". In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-5195.
Texto completo da fonteRelatórios de organizações sobre o assunto "3D culture model"
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Malik, Abir, D. Lam, H. A. Enright, S. K. G. Peters, B. Petkus e N. O. Fischer. Characterizing the Phenotypes of Brain Cells in a 3D Hydrogel Cell Culture Model. Office of Scientific and Technical Information (OSTI), agosto de 2018. http://dx.doi.org/10.2172/1466140.
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Palamar, Svitlana P., Ganna V. Bielienka, Tatyana O. Ponomarenko, Liudmyla V. Kozak, Liudmyla L. Nezhyva e Andrei V. Voznyak. Formation of readiness of future teachers to use augmented reality in the educational process of preschool and primary education. CEUR Workshop Proceedings, julho de 2021. http://dx.doi.org/10.31812/123456789/4636.
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The article substantiates the importance of training future teachers to use AR technologies in the educational process of preschool and primary education. Scientific sources on the problem of AR application in education are analyzed. Possibilities of using AR in work with preschoolers and junior schoolchildren are considered. Aspects of research of the problem of introduction of AR in education carried out by modern foreign and domestic scientists are defined, namely: use of AR-applications in education; introduction of 3D technologies, virtual and augmented reality in the educational process of preschool and primary school; 3D, virtual and augmented reality technologies in higher education; increase of the efficiency of learning and motivating students through the use of AR-applications on smartphones; formation of reading culture by means of augmented reality technology; prospects for the use of augmented reality within the linguistic and literary field of preschool and primary education. The authors analyzed the specifics of toys with AR-applications, interactive alphabets, coloring books, encyclopedias and art books of Ukrainian and foreign writers, which should be used in working with children of preschool and primary school age; the possibilities of books for preschool children created with the help of augmented reality technologies are demonstrated. The relevance of the use of AR for the effective education and development of preschoolers and primary school children is determined. Problems in the application of AR in the educational process of modern domestic preschool education institutions are outlined. A method of diagnostic research of the level and features of readiness of future teachers to use AR in the educational process of preschool and primary education has been developed. Criteria, indicators are defined, the levels of development of the main components of the studied readiness (motivational, cognitive, activity) are characterized. The insufficiency of its formation in future teachers in the field of preschool and primary education; inconsistency between the peculiarities of training future teachers to use AR in professional activities and modern requirements for the quality of the educational process; the need to develop and implement a holistic system of formation of the studied readiness of future teachers in the conditions of higher pedagogical education are proved. A model of forming the readiness of future teachers to use AR in the educational process of preschool and primary education has been developed.
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Yue, Xiaoshan, e Amanda B. Hummon. Proteomic Analysis to Identify Functional Molecules in Drug Resistance Caused by E-Cadherin Knockdown in 3D-Cultured Colorectal Cancer Models. Fort Belvoir, VA: Defense Technical Information Center, setembro de 2013. http://dx.doi.org/10.21236/ada599355.
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Heitman, Joshua L., Alon Ben-Gal, Thomas J. Sauer, Nurit Agam e John Havlin. Separating Components of Evapotranspiration to Improve Efficiency in Vineyard Water Management. United States Department of Agriculture, março de 2014. http://dx.doi.org/10.32747/2014.7594386.bard.
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Vineyards are found on six of seven continents, producing a crop of high economic value with much historic and cultural significance. Because of the wide range of conditions under which grapes are grown, management approaches are highly varied and must be adapted to local climatic constraints. Research has been conducted in the traditionally prominent grape growing regions of Europe, Australia, and the western USA, but far less information is available to guide production under more extreme growing conditions. The overarching goal of this project was to improve understanding of vineyard water management related to the critical inter-row zone. Experiments were conducted in moist temperate (North Carolina, USA) and arid (Negev, Israel) regions in order to address inter-row water use under high and low water availability conditions. Specific objectives were to: i) calibrate and verify a modeling technique to identify components of evapotranspiration (ET) in temperate and semiarid vineyard systems, ii) evaluate and refine strategies for excess water removal in vineyards for moist temperate regions of the Southeastern USA, and iii) evaluate and refine strategies for water conservation in vineyards for semi-arid regions of Israel. Several new measurement and modeling techniques were adapted and assessed in order to partition ET between favorable transpiration by the grapes and potentially detrimental water use within the vineyard inter-row. A micro Bowen ratio measurement system was developed to quantify ET from inter-rows. The approach was successful at the NC site, providing strong correlation with standard measurement approaches and adding capability for continuous, non-destructive measurement within a relatively small footprint. The environmental conditions in the Negev site were found to limit the applicability of the technique. Technical issues are yet to be solved to make this technique sufficiently robust. The HYDRUS 2D/3D modeling package was also adapted using data obtained in a series of intense field campaigns at the Negev site. The adapted model was able to account for spatial variation in surface boundary conditions, created by diurnal canopy shading, in order to accurately calculate the contribution of interrow evaporation (E) as a component of system ET. Experiments evaluated common practices in the southeastern USA: inter-row cover crops purported to reduce water availability and thereby favorably reduce grapevine vegetative growth; and southern Israel: drip irrigation applied to produce a high value crop with maximum water use efficiency. Results from the NC site indicated that water use by the cover crop contributed a significant portion of vineyard ET (up to 93% in May), but that with ample rainfall typical to the region, cover crop water use did little to limit water availability for the grape vines. A potential consequence, however, was elevated below canopy humidity owing to the increased inter-row evapotranspiration associated with the cover crops. This creates increased potential for fungal disease occurrence, which is a common problem in the region. Analysis from the Negev site reveals that, on average, E accounts for about10% of the total vineyard ET in an isolated dripirrigated vineyard. The proportion of ET contributed by E increased from May until just before harvest in July, which could be explained primarily by changes in weather conditions. While non-productive water loss as E is relatively small, experiments indicate that further improvements in irrigation efficiency may be possible by considering diurnal shading effects on below canopy potential ET. Overall, research provided both scientific and practical outcomes including new measurement and modeling techniques, and new insights for humid and arid vineyard systems. Research techniques developed through the project will be useful for other agricultural systems, and the successful synergistic cooperation amongst the research team offers opportunity for future collaboration.