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Artigos de revistas sobre o tema "ADN recombiné – Embryons"

Autor: Grafiati
Publicado: 21 de dezembro de 2024
Última modificação: 31 de julho de 2025

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1

Liu, J., C. Long, M. Westhusin, and D. Kraemer. "44 ATTEMPTS TO USE SOMATIC CELLS ISOLATED FROM FROZEN BOVINE SEMEN FOR NUCLEAR TRANSFER." Reproduction, Fertility and Development 21, no. 1 (2009): 122. http://dx.doi.org/10.1071/rdv21n1ab44.

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Somatic cells in semen are a potential source of nuclei for nuclear transfer to produce genetically identical animals. This is especially important when an animal has died and the only viable genetic material available is frozen semen. In our previous studies, epithelial cells were cultured from fresh ovine and bovine ejaculates and blastocyst stage embryos were produced using these cells (Liu J et al. 2007 Biol. Reprod. special issue, 177; Liu J et al. 2008 Reprod. Fertil. Dev. 20, 102). However, growing cells from frozen semen can be difficult. We hypothesized that nuclei of the somatic cell
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2

Gong-Jin, Wang, Tan Xiao-Dong, Zhou Xiao-Long, Xu Xiao-Bo, and Fan Bi-Qin. "In vitro fertilization and cleavage of mouse oocytes recombined with the first polar body." Chinese Journal of Agricultural Biotechnology 5, no. 2 (2008): 169–73. http://dx.doi.org/10.1017/s1479236208002143.

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AbstractThe developmental functions of oocytes of three strains of mice (Kunming, ICR and C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb) recombined with the nuclei of first polar bodies (Pbs I) were explored. Cumulus oocyte complexes (COCs) from the mice were collected after superovulation, then Pbs I were obtained from the COCs by 2% pronase treatment. The survival of Pbs I under different temperatures was identified by morphology and trypan blue staining. Later, the polar body I (Pb 1) nucleus and a little cytoplasm was injected into each oocyte, the nuclei of which had been enucleated by micromanipu
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Tominaga, Kentaro, Dan Kechele, Guillermo Sanchez, et al. "GENERATION OF HUMAN INTESTINAL ORGANOIDS CONTAINING TISSUE-RESIDENT IMMUNE CELLS." Inflammatory Bowel Diseases 28, Supplement_1 (2022): S57. http://dx.doi.org/10.1093/ibd/izac015.090.

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Abstract BACKGROUND Human gastrointestinal (GI) organoid technologies have transformed GI disease research. By recapitulating the essential steps that occur during embryonic organ development, we could generate in vitro human colonic organoids (HCOs) (Munera et al, Cell Stem Cell. 2017) and human intestinal organoids (HIOs) (Spence et al, Nature. 2011) from iPSCs. Interestingly, HCOs contain both epithelial and surrounding mesenchymal derivatives, including myofibroblasts and tissue-resident immune cells. Our preliminary data demonstrate that HCOs co-develop CD163 positive macrophages derived
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Minokawa, Takuya, and Shonan Amemiya. "Mesodermal Cell Differentiation in Echinoid Embryos Derived from the Animal Cap Recombined with a Quartet of Micromeres." Zoological Science 15, no. 4 (1998): 541–45. http://dx.doi.org/10.2108/0289-0003(1998)15[541:mcdiee]2.0.co;2.

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Minokawa, Takuya, and Shonan Amemiya. "Mesodermal Cell Differentiation in Echinoid Embryos Derived from the Animal Cap Recombined with a Quartet of Micromeres." ZOOLOGICAL SCIENCE 15, no. 4 (1998): 541–45. http://dx.doi.org/10.2108/zsj.15.541.

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6

Toren, Eliana, Yanping Liu, and Chad Hunter. "The SSBP3 Co-Regulator Is a Novel Driver of Islet Cell Structure and Function." Journal of the Endocrine Society 5, Supplement_1 (2021): A327. http://dx.doi.org/10.1210/jendso/bvab048.667.

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Abstract The activities of transcriptional complexes drive the proper development and function of insulin producing beta-cells, ultimately required for the regulation of glucose homeostasis. Our prior work helped to establish that the LIM-homeodomain transcription factor (TF), Islet-1 (Isl1), directly interacts with the Ldb1 co-regulator in developing and adult beta-cells. We further found that a member of the Single Stranded DNA-Binding Protein (SSBP) co-regulator family, SSBP3, interacts with the Isl1:Ldb1 complex in beta-cells and primary islets to impact critical target genes MafA and Glp1
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7

Martinez, Julien, Lisa Klasson, John J. Welch, and Francis M. Jiggins. "Life and Death of Selfish Genes: Comparative Genomics Reveals the Dynamic Evolution of Cytoplasmic Incompatibility." Molecular Biology and Evolution 38, no. 1 (2020): 2–15. http://dx.doi.org/10.1093/molbev/msaa209.

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Abstract Cytoplasmic incompatibility is a selfish reproductive manipulation induced by the endosymbiont Wolbachia in arthropods. In males Wolbachia modifies sperm, leading to embryonic mortality in crosses with Wolbachia-free females. In females, Wolbachia rescues the cross and allows development to proceed normally. This provides a reproductive advantage to infected females, allowing the maternally transmitted symbiont to spread rapidly through host populations. We identified homologs of the genes underlying this phenotype, cifA and cifB, in 52 of 71 new and published Wolbachia genome sequenc
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Nakayama, Takuya, Amanda Cox, Mary Howell, and Robert M. Grainger. "Gynogenetic Production of Embryos inXenopus tropicalisUsing a Cold Shock Procedure: Rapid Screening Method for Gene Editing Phenotypes." Cold Spring Harbor Protocols 2022, no. 12 (2022): pdb.prot107648. http://dx.doi.org/10.1101/pdb.prot107648.

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Gynogenesis is a form of parthenogenesis in which eggs require sperm for fertilization but develop to adulthood without the contribution of paternal genome information, which happens naturally in some species. InXenopus, gynogenetic diploid animals can be made experimentally. In mutagenesis strategies that only generate one allele of a recessive mutation, as might occur during gene editing, gynogenesis can be used to quickly reveal a recessive phenotype in eggs carrying a recessive mutation, thereby skipping one generation normally required to screen by conventional genetics.Xenopusoocytes do
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Ye, L., R. Mayberry, E. Stanley, A. Elefanty, and C. Gargett. "134. DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO MULLERIAN TISSUE." Reproduction, Fertility and Development 22, no. 9 (2010): 52. http://dx.doi.org/10.1071/srb10abs134.

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The human uterus develops from the distal Mullerian Duct, a derivative of the mesoderm germ layer. Unlike other mammalian species (eg. mouse) the endometrium of the human uterus develops prenatally during gestation. Little is known about the developmental process involved. A better understanding of human endometrial development may shed light on the mechanisms involved in endometrial regeneration and pathogenesis of adult proliferative endometrial diseases. Mouse neonatal uterine mesenchyme (mNUM) is inductive and can maintain the phenotype of normal adult human endometrial epithelial cells [1
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10

Álvarez-Aznar, A., I. Martínez-Corral, N. Daubel, C. Betsholtz, T. Mäkinen, and K. Gaengel. "Tamoxifen-independent recombination of reporter genes limits lineage tracing and mosaic analysis using CreERT2 lines." Transgenic Research 29, no. 1 (2019): 53–68. http://dx.doi.org/10.1007/s11248-019-00177-8.

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Abstract The CreERT2/loxP system is widely used to induce conditional gene deletion in mice. One of the main advantages of the system is that Cre-mediated recombination can be controlled in time through Tamoxifen administration. This has allowed researchers to study the function of embryonic lethal genes at later developmental timepoints. In addition, CreERT2 mouse lines are commonly used in combination with reporter genes for lineage tracing and mosaic analysis. In order for these experiments to be reliable, it is crucial that the cell labeling approach only marks the desired cell population
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Ishii, Kenichiro, Tetsuya Imamura, Kazuhiro Iguchi, et al. "Evidence that androgen-independent stromal growth factor signals promote androgen-insensitive prostate cancer cell growth in vivo." Endocrine-Related Cancer 16, no. 2 (2009): 415–28. http://dx.doi.org/10.1677/erc-08-0219.

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Activation of tumor–stromal interactions is considered to play a critical role in the promotion of tumorigenesis. To discover new therapeutic targets for hormone-refractory prostate tumor growth under androgen ablation therapy, androgen-sensitive LNCaP cells and the derived sublines, E9 (androgen-low-sensitive), and AIDL (androgen-insensitive), were recombined with androgen-dependent embryonic rat urogenital sinus mesenchyme (UGM). Tumors of E9+UGM and AIDL+UGM were approximately three times as large as those of LNCaP+UGM. Tumors grown in castrated hosts exhibited reduced growth as compared wi
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12

Zheng, Yunfei, Lingfei Jia, Pengfei Liu, et al. "Insight into the maintenance of odontogenic potential in mouse dental mesenchymal cells based on transcriptomic analysis." PeerJ 4 (February 22, 2016): e1684. http://dx.doi.org/10.7717/peerj.1684.

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Background.Mouse dental mesenchymal cells (mDMCs) from tooth germs of cap or later stages are frequently used in the context of developmental biology or whole-tooth regeneration due to their odontogenic potential.In vitro-expanded mDMCs serve as an alternative cell source considering the difficulty in obtaining primary mDMCs; however, cultured mDMCs fail to support tooth development as a result of functional failures of specific genes or pathways. The goal of this study was to identify the genes that maintain the odontogenic potential of mDMCs in culture.Methods.We examined the odontogenic pot
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13

Choi, Y. H., J. D. Norris, I. C. Velez, C. C. Jacobson, D. L. Hartman, and K. Hinrichs. "32 A CLONED FOAL PRODUCED USING OOCYTES RECOVERED BY TRANSVAGINAL ASPIRATION OF IMMATURE FOLLICLES." Reproduction, Fertility and Development 23, no. 1 (2011): 122. http://dx.doi.org/10.1071/rdv23n1ab32.

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Closure of all the horse slaughterhouses in the US has reduced the availability of equine oocytes in this country. We investigated the use of oocytes collected from immature follicles of live mares for cloning research. Because blastocyst development of equine cloned embryos is typically low (<10%), we also investigated the effect of Scriptaid, a histone deacetylase inhibitor that increases blastocyst development, live birth rate, and neonatal health in cloned mice and pigs. Immature oocytes were transvaginally aspirated from all follicles ≥8 mm diameter in a herd of 11 mares. The oocytes w
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14

Soreq, Hermona. "Novel single‐nucleus transcriptomics unravels developmental and functionally controlled principles of mammalian neuromuscular junctions." Journal of Neurochemistry, November 9, 2023. http://dx.doi.org/10.1111/jnc.15986.

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AbstractProf Ohno's team (Ohkawara et al. 2023, current issue) underscored the dynamic and functional features that co‐shape the embryonic and early post‐natal development of mammalian neuromuscular junctions (NMJs) using single‐nucleus transcriptomics which provides specific insights into the activities of individually studied nuclei and their functional characteristics. Unlike other single‐nucleus transcriptomics studies, which tend to be limited to single developmental time points, this article provides novel views of the complex developmental and regulatory dynamics and embryonic cell type
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15

sprotocols. "Preparing Recombinant Gonad Organ Cultures." December 30, 2014. https://doi.org/10.5281/zenodo.13628.

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Authors: Blanche Capel and Jordan Batchvarov Corresponding author ([b.capel@cellbio.duke.edu](b.capel@cellbio.duke.edu)) ### INTRODUCTION It can be useful to assay migration between any two adjacent tissues during development. This protocol assays cell migration between the gonad and mesonephros using tissue recombination between genetically marked and unmarked tissue, combined with an organ culture technique. First, agar blocks are prepared in a custom-built mold. The size and shape of the wells are important to maintain the authentic three-dimensional morphology of the organ; the molds here
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