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Teses / dissertações sobre o tema "Analyse RNAseq"

Autor: Grafiati

Publicado: 2 de novembro de 2024

Última modificação: 31 de julho de 2025

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1

Benoit-Pilven, Clara. "Analyse de l’épissage alternatif dans les données RNAseq : développement et comparaison d’outils bioinformatiques." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1280/document.

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L'épissage alternatif est un processus biologique qui génère la diversité du protéome malgré le nombre limité de gène. Ce mécanisme régule à la fois les gènes de manières qualitatives (isoformes exprimées) mais aussi quantitatives (niveau d'expression). Avec le développement des technologies de séquençage à haut débit, il est maintenant possible d'étudier à large échelle les aspects quantitatif et qualitatif du transcriptome avec une même expérience (RNA-seq). Durant ma thèse, j'ai développé une nouvelle méthode d'analyse de l'épissage alternatif dans les données RNA-seq. J'ai également partic

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2

Meunier, Léa. "Analyse de signatures transcriptomiques et épigénétiques des carcinomes hépatocellulaires." Thesis, Université de Paris (2019-....), 2020. http://www.theses.fr/2020UNIP7082.

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Élucider les processus transcriptionnels et épigénétiques dérégulés dans les cancers est fondamental pour mieux comprendre les voies biologiques impliquées et proposer une thérapie adaptée au phénotype moléculaire de chaque tumeur. Les approches classiques de classification non supervisée définissent des groupes moléculaires principaux pour chaque type tumoral. Cependant, ces méthodes, appliquées à des tumeurs complexes comme le carcinome hépatocellulaire (CHC), le 3ème cancer le plus mortel au monde, définissent des groupes qui restent relativement hétérogènes et ne reflètent qu’imparfaitemen

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Riquier, Sébastien. "Dans les abysses du transcriptome : découverte de nouveaux biomarqueurs de cellules souches mésenchymateuses par analyse approfondie du RNAseq." Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTT004.

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Le développement du séquençage ARN, ou RNAseq, a permis l'essor de la recherche intensive de biomarqueurs dans de nombreux domaines de la biologie. L’information complète du transcriptome contenue dans les données de sorties, permet à un bioinformaticien assidu de dépasser les connaissances actuelles et d’accéder, grâce à des pipelines informatiques avancés, à d’innombrables signatures d’intérêts inédites. Dans cette thèse nous mettons en avant que ces marqueurs potentiels, essentiellement explorés pour répondre à des problématiques clinique en conditions pathologiques, peuvent être utilisés p

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4

Gonzalez, Claudia. "Étude des mécanismes immunitaires impliqués dans le contrôle de l'infection par le virus Nipah." Electronic Thesis or Diss., Lyon, École normale supérieure, 2024. http://www.theses.fr/2024ENSL0035.

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Le virus Nipah (NiV) est un paramyxovirus hautement pathogène pour les humains faisant partie de la liste prioritaire pour la recherche et le développement de l’OMS. Les chauves-souris Pteropus sont le réservoir naturel asymptomatique du NiV et nous nous sommes intéressés aux mécanismes leur permettant de contrôler l’infection. Pour cela, nous avons réalisé une analyse transcriptomique comparative entre des cellules de chauves-souris et des cellules humaines. Nous avons tout d’abord observé des profils immunitaires innés distincts à l’état basal. Les cellules de chauves-souris présentent des n

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5

Gössringer, Markus. "In-vivo-Analysen zur Funktion bakterieller RNase-P-Proteine in Bacillus subtilis." [S.l. : s.n.], 2004. http://archiv.ub.uni-marburg.de/diss/z2004/0529/.

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6

Ahmed, Firdous. "Identification of potential biomarkers in lung cancer as possible diagnostic agents using bioinformatics and molecular approaches." University of the Western Cape, 2015. http://hdl.handle.net/11394/4862.

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>Magister Scientiae - MSc<br>Lung cancer remains the leading cause of cancer deaths worldwide, with the majority of cases attributed to non-small cell lung carcinomas. At the time of diagnosis, a large percentage of patients present with advanced stage of disease, ultimately resulting in a poor prognosis. The identification circulatory markers, overexpressed by the tumour tissue, could facilitate the discovery of an early, specific, non-invasive diagnostic tool as well as improving prognosis and treatment protocols. The aim was to analyse gene expression data from both microarray and RNA seque

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7

Mary, Catherine. "Utilisation séquentielle des sites accepteurs d'épissage lors de l'expression du provirus HIV-1 : analyse par cartographie à la RNAse." Lyon 1, 1994. http://www.theses.fr/1994LYO1T236.

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8

Ahmed, Fathima Zuba. "Unravelling genes responsible for successful anthocyanin production in Nicotiana benthamiana." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/230763/1/Fathima%20Zuba_Ahmed_Thesis.pdf.

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This thesis utilised two approaches, forward genetics and comparative transcriptomic analysis, to investigate the contrasting response to anthocyanin production observed in two distinct Nicotiana benthamiana ecotypes, LAB and QLD. The thesis is a step forward in utilising N. benthamiana as a candidate in forward genetics, currently limited due to its large complex genome and polyploid nature. The study utilised a cross-population between LAB and QLD to investigate the nature of inheritance of the contrasting parental phenotypes in its progeny. Additionally, expression profiles of anthocyanin b

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9

Marchant, Axelle. "Le processus de domiciliation des punaises hématophages vectrices de la maladie de Chagas : apport de l’étude du transcriptome chimiosensoriel." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS008/document.

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En Amérique Latine, les punaises hématophages Triatominae transmettent à l’homme le parasite Trypanosoma cruzi, responsable de la maladie de Chagas touchant actuellement 5 millions de personnes. Même si les programmes d’éradication chimique des vecteurs sont efficaces, la maladie persiste du fait de la recolonisation des habitations humaines par des vecteurs provenant d’habitats naturels. Ainsi, certaines espèces présentent une capacité d’adaptation aux anthroposystèmes (processus de domiciliation), alors que d’autres espèces apparentées ne l’ont pas. Comprendre cette capacité d’adaptation est

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10

Loe-mie, Yann. "Contribution bioinformatique à l' analyse du transcriptome humain." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM4002/document.

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Dans la première partie j'ai analysé des jeux de données de RNA-seq de transcriptome de petits ARNs disponibles dans les bases de données publiques. J'y ai observé 2 points intrigants : - une grande partie des lectures (bien que courtes) ne peux pas être alignée sur le génome de référence sans discordance et cette fraction non-alignable est parfois majoritaire. - de nombreuses lectures ont des tailles autours de 15-18nt qui ne correspondent à aucun type de petits ARNs connues, cette fraction est également majoritaires dans certains cas. Ces expériences sont souvent conçues pour la détection de

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11

Bosch, Andreas [Verfasser], and Michael [Akademischer Betreuer] Thomm. "Analyse von kleinen RNAs mit RNA-bindenden Proteinen in Pyrococcus furiosus / Andreas Bosch. Betreuer: Michael Thomm." Regensburg : Universitätsbibliothek Regensburg, 2014. http://d-nb.info/1054191646/34.

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12

Hillebrand, Arne Thomas. "Funktionelle Analyse kleiner, nichtkodierender RNAs in den Organellen von Plasmodium falciparum und Charakterisierung neuer RNA-Bindeproteine in Apicomplexa." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/20433.

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Die Infektionskrankheit Malaria wird von einzelligen Parasiten der Gattung Plasmodium verursacht und stellt vor allem im südlichen Afrika eine große Herausforderung dar. Die Zellen der Parasiten enthalten zwei endosymbiontische Organellen, den Apicoplast und das Mitochondrium. Beide Organellen besitzen ein reduziertes Genom. Die Struktur des mitochondrialen Genoms ist ungewöhnlich. Mit nur 6 kb gehört es zu den kleinsten beschriebenen Genomen und enthält neben drei proteinkodierende Gene auch 34 kleine rRNA-Gene. Um das Genom zu exprimieren wird eine Vielzahl von kernkodierten Faktoren benötig

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13

Köhler, Karen [Verfasser], and Roland Karl [Akademischer Betreuer] Hartmann. "Mechanistic and structural analyses of different non-coding RNAs in bacteria / Karen Köhler. Betreuer: Roland Karl Hartmann." Marburg : Philipps-Universität Marburg, 2014. http://d-nb.info/1059855682/34.

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14

Findeiß, Sven. "Expanding the repertoire of bacterial (non-)coding RNAs." Doctoral thesis, Universitätsbibliothek Leipzig, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-67816.

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The detection of non-protein-coding RNA (ncRNA) genes in bacteria and their diverse regulatory mode of action moved the experimental and bio-computational analysis of ncRNAs into the focus of attention. Regulatory ncRNA transcripts are not translated to proteins but function directly on the RNA level. These typically small RNAs have been found to be involved in diverse processes such as (post-)transcriptional regulation and modification, translation, protein translocation, protein degradation and sequestration. Bacterial ncRNAs either arise from independent primary transcripts or their mature

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15

Cavé-Radet, Armand. "Évolution de la tolérance aux Hydrocarbures Aromatiques Polycycliques (HAPs) chez les spartines polyploïdes : analyses physiologiques et régulations transcriptomiques par les micro-ARNs." Thesis, Rennes 1, 2018. http://www.theses.fr/2018REN1B064/document.

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Cette étude vise à explorer les mécanismes de tolérance des plantes aux xénobiotiques organiques de la famille des HAPs (phénanthrène), à travers l’analyse de l’impact des évènements de spéciation par hybridation et duplication génomique (allopolyploïdie). Nous avons pour cela mené une approche comparative sur un modèle de spéciation allopolyploïde récente, constitué des espèces parentales hexaploïdes S. alterniflora et S. maritima, et de l’allopolyploïde S. anglica qui résulte de la duplication du génome de leur hybride F1 S. x townsendii. Une approche intégrative basée sur des analyses physi

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16

Li, Siwei. "High Throughput Automated Comparative Analysis of RNAs Using Isotope Labeling and LC-MS/MS." University of Cincinnati / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1384427990.

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17

Bajak, Edyta Zofia. "Genotoxic stress: novel biomarkers and detection methods : uncovering RNAs role in epigenetics of carcinogenesis /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-415-5/.

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18

Hillebrand, Arne Thomas [Verfasser], Christian [Gutachter] Schmitz-Linneweber, Kai [Gutachter] Matuschewski, and Volker [Gutachter] Knoop. "Funktionelle Analyse kleiner, nichtkodierender RNAs in den Organellen von Plasmodium falciparum und Charakterisierung neuer RNA-Bindeproteine in Apicomplexa / Arne Thomas Hillebrand ; Gutachter: Christian Schmitz-Linneweber, Kai Matuschewski, Volker Knoop." Berlin : Humboldt-Universität zu Berlin, 2019. http://d-nb.info/1193989310/34.

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19

Tufail, Muhammad Aammar. "Use of plant growth promoting endophytic bacteria to alleviate the effects of individual and combined abiotic stresses on plants as an innovative approach to discover new delivery strategies for bacterial bio-stimulants." Doctoral thesis, Università degli studi di Trento, 2021. http://hdl.handle.net/11572/305571.

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Bacterial endophytes are the organisms that live inside the plant for a full or a part of their life cycle. Endophytic bacteria have captured the interest of agriculture industry due to their plant beneficial properties, such as synthesis of phytohormones, solubilization of soil nutrients, and alleviation of biotic and abiotic stresses. Several studies have reported that stress tolerant endophytic bacteria can work with a similar performance as non-stressed conditions when inoculated to the plants under stressed conditions. Combination of abiotic stresses such as salinity, drought and low nitr

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20

Geißen, René [Verfasser], Rolf [Akademischer Betreuer] Wagner, and Michael [Akademischer Betreuer] Feldbrügge. "Analyse der physiologischen Rolle der 6S RNA aus Escherichia coli und Vergleich der molekularen Mechanismen zwischen 6S RNAs aus E. coli und Cyanobakterien / René Geißen. Gutachter: Michael Feldbrügge. Betreuer: Rolf Wagner." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2011. http://d-nb.info/101543472X/34.

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21

Aghamirzaie, Delasa. "Isoform-Specific Expression During Embryo Development in Arabidopsis and Soybean." Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/73054.

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Almost every precursor mRNA (pre-mRNA) in a eukaryotic organism undergoes splicing, in some cases resulting in the formation of more than one splice variant, a process called alternative splicing. RNA-Seq provides a major opportunity to capture the state of the transcriptome, which includes the detection of alternative spicing events. Alternative splicing is a highly regulated process occurring in a complex machinery called the spliceosome. In this dissertation, I focus on identification of different splice variants and splicing factors that are produced during Arabidopsis and soybean embryo d

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22

Soumya, R. Deo. "Characterization of the terminal region RNAs of the West Nile virus genome and their interaction with the small isoform of 2' 5'-oligoadenylate synthetases (OAS)." Plos.org, 2014. http://hdl.handle.net/1993/30733.

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2'-5'-oligoadenylate synthetases (OAS) are interferon-stimulated proteins that act in the innate immune response to viral infection. Upon binding to viral double-stranded RNAs, OAS enzymes produce 2'-5'-linked oligoadenylates that stimulate RNase L and ultimately slow viral propagation. Studies have linked mutations in the OAS1 gene to increased susceptibility to West Nile virus (WNV) infection, highlighting the importance of the OAS1 enzyme. Here I report that the 5'-terminal region (5'-TR) of the WNV genome, comprising both the 5'-untranslated region (5'-UTR) and initial coding region, is ca

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23

Tufail, Muhammad Aammar. "Use of plant growth promoting endophytic bacteria to alleviate the effects of individual and combined abiotic stresses on plants as an innovative approach to discover new delivery strategies for bacterial bio-stimulants." Doctoral thesis, Università degli studi di Trento, 2021. http://hdl.handle.net/11572/305571.

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Bacterial endophytes are the organisms that live inside the plant for a full or a part of their life cycle. Endophytic bacteria have captured the interest of agriculture industry due to their plant beneficial properties, such as synthesis of phytohormones, solubilization of soil nutrients, and alleviation of biotic and abiotic stresses. Several studies have reported that stress tolerant endophytic bacteria can work with a similar performance as non-stressed conditions when inoculated to the plants under stressed conditions. Combination of abiotic stresses such as salinity, drought and low nitr

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24

Van, Den Elzen Antonia. "Etude du complexe Dom34-Hbs1 ressemblant aux facteurs de terminaison : analyse fonctionnelle de ses rôles dans le contrôle qualité des ARN et dans la stimulation de la traduction par dissociation des ribosomes inactifs." Thesis, Strasbourg, 2013. http://www.theses.fr/2013STRAJ110/document.

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Après un cycle de la production des protéines, les sous-unités des ribosomes terminés sont dissociés, afin de les rendre disponibles pour de nouveaux cycles de traduction. Si lors de la traduction le ribosome pause, il ne pourra pas terminer la traduction et être recyclé par la voie classique. Un mécanisme de recyclage alternatif a évolué pour dissocier de tels ribosomes arrêtés. Un complexe composé des facteurs Dom34 et Hbs1 induit leur dissociation. Ce complexe est aussi impliqué dans des voies de contrôle qualité qui ciblent des ARN qui causent des arrêts ribosomiques. Dans cette thèse, l'i

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Moraes, Rodrigo de. "Uma investigação empírica e comparativa da aplicação de RNAs ao problema de mineração de opiniões e análise de sentimentos." Universidade do Vale do Rio dos Sinos, 2013. http://www.repositorio.jesuita.org.br/handle/UNISINOS/3411.

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Submitted by Silvana Teresinha Dornelles Studzinski (sstudzinski) on 2015-05-04T17:25:43Z No. of bitstreams: 1 Rodrigo Morais.pdf: 5083865 bytes, checksum: 69563cc7178422ac20ff08fe38ee97de (MD5)<br>Made available in DSpace on 2015-05-04T17:25:43Z (GMT). No. of bitstreams: 1 Rodrigo Morais.pdf: 5083865 bytes, checksum: 69563cc7178422ac20ff08fe38ee97de (MD5) Previous issue date: 2013<br>Nenhuma<br>A área de Mineração de Opiniões e Análise de Sentimentos surgiu da necessidade de processamento automatizado de informações textuais referentes a opiniões postadas na web. Como principal motivaçã

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Ferrarini, Margherita. "From exome to whole genome sequencing: mining for inconsistencies and functional elements in coding and non-coding regions." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3424933.

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Over the last two decades the advancement in DNA sequencing technologies has enormously increased the amount of sequencing data available to researchers and geneticists. This has been accompanied by the development of tools for sequencing data analysis, including the human reference genome, that is undoubtedly an indispensable resource. It is known that the reference genome does not always represent the real consensus sequence of the human population, due to the inclusion of rare alleles and sequencing errors. Moreover, genomic duplications are often misassembled and, as a result, they may be

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27

Laugier, Laurie. "Identification de marqueurs de susceptibilité dans les formes chroniques de la maladie de Chagas." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0226.

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La maladie de Chagas est une maladie parasitaire causée par le protozoaire Trypanosoma cruzi et transmise par des insectes hématophages . Elle est composée de 2 phases : la phase aiguë et la phase chronique. Parmi les individus infectés, 30 % développent la forme chronique de la maladie. Les patients présentent des atteintes cardiaques, digestives (œsophage, côlon) et cardiodigestives. Notre étude a été focalisée sur les patients atteints de cardiomyopathie chagasique (CCC). Notre objectif est d’identifier des gènes de susceptibilité pouvant être impliqués dans le développement des formes chro

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28

Guidi, Mònica. "Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3)." Doctoral thesis, Universitat Pompeu Fabra, 2009. http://hdl.handle.net/10803/7114.

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Neurotrophins and their receptors are key molecules in the development of the<br/>nervous system. Neurotrophin-3 binds preferentially to its high-affinity receptor<br/>NTRK3, which exists in two major isoforms in humans, the full-length kinaseactive<br/>form (150 kDa) and a truncated non-catalytic form (50 kDa). The two<br/>variants show different 3'UTR regions, indicating that they might be differentially<br/>regulated at the post-transcriptional level. In this work we explore how<br/>microRNAs take part in the regulation of full-length and truncated NTRK3,<br/>demonstrating that the two isof

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Dadkhahi, Sara [Verfasser]. "Expression von extrazellulärer RNase und Analyse ihrer Aktivität in Endothelzellen / vorgelegt von Sara Dadkhahi." 2008. http://d-nb.info/990946339/34.

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Fu, Yu. "Computational analyses of small silencing RNAs." Thesis, 2018. https://hdl.handle.net/2144/33235.

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High-throughput sequencing is a powerful tool to study diverse aspects of biology and applies to genome, transcriptome, and small RNA profiling. Ever increasing sequencing throughput and more specialized sequencing assays demand more sophisticated bioinformatics approaches. In this thesis, I present 4 studies for which I developed computational methods to handle high-throughput sequencing data to gain insights into biology. The first study describes the genome of High Five (Hi5) cells, originally derived from Trichoplusia ni eggs. The chromosome-level assembly (scaffold N50 = 14.2 Mb) cont

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Βρυζάκη, Ελευθερία. "Η ριβονουκλεάση Ρ (RNase P) των ανθρώπινων λεμφοκυττάρων". Thesis, 2008. http://nemertes.lis.upatras.gr/jspui/handle/10889/1596.

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Η ριβονουκλεάση P (RNase P) είναι το ένζυμο που ευθύνεται για την ωρίμανση του 5΄ άκρου των πρόδρομων μορίων tRNA συμμετέχοντας έτσι στην πρωτεΐνοσύνθεση. H RNase P καταλύει την ενδονουκλεολυτική θραύση ενός φωσφοδιεστερικού δεσμού παρουσία ιόντων Mg2+ παράγοντας προϊόντα με 3΄ υδροξυλικά και 5΄ φωσφορικά άκρα. Η πλειοψηφία των RNase P ενζύμων που έχουν μελετηθεί μέχρι σήμερα είναι ριβονουκλεοπρωτεΐνες αποτελούμενες από μια RNA και πρωτεϊνικές υπομονάδες. Η RNA υπομονάδα της βακτηριακής RNase P είναι ένα από τα πρώτα καταλυτικά RNA που μελετήθηκαν. Η RNase P και το ριβόσωμα είναι τα μόνα γνω

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Gößringer, Markus [Verfasser]. "In-vivo-Analysen zur Funktion bakterieller RNase-P-Proteine in Bacillus subtilis / vorgelegt von Markus Gößringer." 2004. http://d-nb.info/973038152/34.

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Dietrich, Sascha. "Analyse und Charakterisierung regulatorischer Vorgänge in Bacillus licheniformis." Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0022-5FD3-B.

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Woodhams, Michael D., Peter F. Stadler, David Penny, and Lesley J. Collins. "RNase MRP and the RNA processing cascade in the eukaryotic ancestor." 2007. https://ul.qucosa.de/id/qucosa%3A31877.

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Background Within eukaryotes there is a complex cascade of RNA-based macromolecules that process other RNA molecules, especially mRNA, tRNA and rRNA. A simple example is the RNase MRP processing of ribosomal RNA (rRNA) in ribosome biogenesis. One hypothesis is that this complexity was present early in eukaryotic evolution; an alternative is that an initial simplified network later gained complexity by gene duplication in lineages that led to animals, fungi and plants. Recently there has been a rapid increase in support for the complexity-early theory because the vast majority of these RNA-proc

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Ferreira, Joana Catarina da Rocha. "Genomic and transcriptomic analyses in cancers related with viral infection." Master's thesis, 2016. http://hdl.handle.net/1822/47439.

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Dissertação de mestrado em Bioinformatics<br>In the past 30 years, accumulated evidence has been supporting viral infection as one factor responsible for 15-20% of human malignancies worldwide (W. S. Liang et al. 2014; McLaughlin-Drubin and Munger 2008). Studies on oncogenic viruses have proved their importance on cellular malfunction along the carcinogenic process, and showed that their association with cancer can amount from 15% to 100% (McLaughlin-Drubin and Munger 2008), depending on the type of tumour. With the large amount of genomic and metagenomic information available on public

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YiLi-Chang and 張怡莉. "Analyses of Replication Signals of Satellite RNAs Associated with Cucumber Mosaic Virus." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/74960729152939618250.

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碩士<br>國立中興大學<br>生物科技學研究所<br>92<br>Cucumber mosaic virus (CMV) is the type species of the genus Cucumovirus. Some strains of CMV harbor satellite RNAs (satRNAs) that are dependent on CMV for their replication, encapsidation, and transmission. The specific aim of this study is to investigate the 5’- and 3’-terminal signals on satRNAs required for high efficiency replication and to distinguish the effects of nucleotide sequences and structures. The 5’- and/or 3’-terminal 30 nucleotides were replaced by random sequences with synthetic primers in polymerase chain reactions (PCR). The transcrip

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horng-woei, Yang, and 楊鴻偉. "Analyse of Phloem Sap Transcription Profile Revealed Two Floral Activators, FVE and AGL24 are Phloem-mobile RNAs." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/17931981243904097436.

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博士<br>國防醫學院<br>生命科學研究所<br>97<br>Plants take advantage of the vascular system to operate environmental stimulates for fine-tuning their developmental programs. Recent evidence shows that the FLOWERING LOCUS T (FT) protein is the long-sought-after florigen that integrates the photoperiod variation perceived in the leaves. However, evidence also supports that other yet-to-be identified systemic regulators participate in floral induction. To this end, we investigated phloem exudates from excised broccoli (Brassica oleracea) inflorescences. Microarray and RT-PCR analyses revealed that at least two

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Chen, Ho-Ming, and 陳荷明. "Exploring the Arabidopsis Non-coding RNAs: Inference from the Bioinformatic Analyses of Small RNA Sequencing Data." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/64874065425101072828.

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博士<br>國立中興大學<br>生物科技學研究所<br>97<br>Non-coding RNAs (ncRNAs) play vital roles in translation, splicing, RNA processing, RNA modification and regulation of gene expression. The advancement in ncRNA discovery is evolving along with the finding of new classes of ncRNAs and the invention of revolutionary sequencing platforms. High-throughput sequencing technologies greatly facilitate the study of small regulatory RNAs which are 20 to 30 nt in length. High-throughput sequencing data of 18-26 nt small RNA fragments are a mixture of small regulatory RNAs and degraded products from coding RNAs or ncRNAs

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Zhan, Shuhua. "Genome-wide expression analysis and regulation of microRNAs and cis natural antisense transcripts in Arabidopsis thaliana." 2012. http://hdl.handle.net/10214/3274.

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Small RNAs (sRNAs), circa 21-26nt RNA molecules, are a novel class of regulatory molecules that influence many aspects of plant biology. The first objective of this thesis was to utilize computational approaches both to investigate how microRNAs (miRNAs), a type of sRNA, as a class affect their target transcripts’ accumulation and to identify novel miRNAs in Arabidopsis thaliana. The second objective of this thesis was to examine the regulation of protein coding (PC) cis natural antisense transcripts (cis-NATs), which have the potential to make double stranded RNA. Computational analysis of t

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