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Teses / dissertações sobre o tema "Blood Progenitor Cells"

Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 3 de fevereiro de 2022

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1

Schütte, Judith. "Analysis of regulatory networks in blood stem/progenitor cells." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648631.

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2

Prasad, Raju. "Endothelial progenitor cells, vascular function, and exercise." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 59 p, 2009. http://proquest.umi.com/pqdweb?did=1654501181&sid=4&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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3

Suresh, Venkatesan. "Release of haemopoietic progenitor cells in peripheral blood after acute myocardial infarction." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490118.

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We aimed to establish whether and over what time course haemopoietic progenitor cells are released in the peripheral circulation after myocardial infarction (MI) in humans. We investigated the expression of CD34+ and novel stem cell marker CD133+ on peripheral blood stem cell and also the time course of their release after ischaemic insult. We also investigated the stem cell expression of various endothelial markers like CD31+ and CD144+. We recruited 5 patients with acute ST segment elevation MI, 5 patients with non-cardiac chest pain and 5 healthy controls. 20ml of peripheral blood was obtai

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4

Ensley, Ann Elizabeth. "Functional evaluation of circulating endothelial progenitor cells for vascular tissue engineering." Diss., Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-04042006-145611/.

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Thesis (Ph. D.)--Biomedical Engineering, Georgia Institute of Technology, 2006.<br>Vito, Raymond, Committee Member ; Nerem, Robert, Committee Chair ; Eskin, Suzanne, Committee Member ; Hanson, Stephen, Committee Member ; Gibbons, Gary, Committee Member.

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5

Greenhowe, Jennifer. "Stem and progenitor cells in wound healing." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:87a9a7a1-b595-458a-913f-64497174f988.

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As more patients with large body surface area burns are surviving and requiring reconstructive surgery, there is a necessity for advances in the provision of bioengineered alternatives to autologous skin cover. The aims of this Thesis are to identify feasible source tissues of Endothelial Colony Forming Cells and Mesenchymal Stem/Stromal Cells for microvascular network formation in vitro with three-dimensional dermal substitute scaffolds. The working hypothesis is that pre-vascularised dermal scaffolds will result in better quality scarring when used with split thickness skin grafts. Human umb

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6

Mitchell, Andrew Joseph. "Understanding the role of endothelial progenitor cells in vascular injury and repair." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/33310.

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Introduction: Vascular injury is the crucial initiating event in atherosclerosis and is universal following percutaneous coronary intervention. The cellular response to this injury largely determines vessel outcome. Endothelial progenitor cells (EPCs) and their progeny, late outgrowth endothelial cells (EOCs) are thought to play an important role in this process and characterising this role would be valuable in better understanding vascular injury and repair. Methods: The radial artery in the context of transradial cardiac catheterisation was examined as a model of vascular injury with charact

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7

Robinson, Scott Thomas. "Determining the role of endothelial progenitor cells in post-natal neovascularization." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37178.

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Endothelial Progenitor Cells (EPCs) were first identified from human blood samples as a population of circulating mononuclear cells capable of displaying a mature endothelial cell phenotype in culture. Subsequent studies have established that EPCs arise from the bone marrow (BM) and incorporate into the endothelium at sites of blood vessel growth, suggesting a potential role for these cells in neovascularization. Furthermore, a decline in EPC count has been correlated to multiple vascular pathologies, indicating that EPC number could serve as a biomarker of cardiovascular disease. Unfortunatel

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8

Krenning, Guido. "Endothelial progenitor cells in vascular regenerative medicine towards 'designer blood vessels' and 'therapeutic neovascularization' /." [S.l. : [Groningen : s.n.] ; University of Groningen] [Host], 2009. http://irs.ub.rug.nl/ppn/.

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9

Joshi, Shrinidh Ashokkumar. "Hypoxic Regulation of Angiotensin-Converting Enzyme 2 and Mas Receptor in Hematopoietic Stem/Progenitor Cells: A Translational Study." Diss., North Dakota State University, 2018. https://hdl.handle.net/10365/28961.

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Vascular disease is the leading cause of mortality and morbidity in the western world, and account for the 1 of every 3 death?s in the US, but a cure for vascular disease is yet to be realized. Hematopoietic stem progenitor cells (HSPCs) are mobilized from bone marrow and have the innate propensity to accelerate vascular repair by reendothelialization and revascularization of ischemic areas. The vasoreparative ability of HSPCs is largely due to their capacity to home to the areas of hypoxia and their sensitivity to hypoxia plays a critical role in the vasoreparative functions of these cells. T

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10

Southcott, Mark. "Expression of blood group antigens on erythroid progenitor cells during differentiation using an in vitro culture system." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297767.

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11

Almoflehi, Sakhar. "Cord Blood CD34+ Expansion Using Vitamin-C: An Epigenetic Regulator." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41413.

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Vitamin-C (Vit-C) has been shown to modulate hematopoietic stem cells and leukemia stem cell frequency in-vivo. Herein, Vit-C analogue, L-ascorbic acid 2-phosphate (AA2P), was investigated as a new potential HSC expansion agonist. Cord blood CD34+ cells were expanded in cultures with or without AA2P. AA2P induced a 2-fold increase in the expansion of stem and progenitor subsets including lymphoid-primed multi-potential progenitors (p<0.05, n=3) and functional colony forming progenitors. The functional properties of AA2P grafts was evaluated with a xenotransplant model. Superior platelet levels

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12

Tan, Kevin S. "Role of Circulating Peripheral Blood-Derived Endothelial Colony-Forming Cells in Patients with Proliferative Diabetic Retinopathy." Case Western Reserve University School of Graduate Studies / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=case1238452739.

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13

Lutteropp, Michael. "The emergence and early fate decisions of stem and progenitor cells in the haematopoietic system." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:eef3e876-bde2-4114-8ac2-bf0c87492a55.

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The alternative road map describes the separation of lympho-myeloid and myeloid-megakaryocyte-erythroid (myeloid-Mk-E) lineages as the earliest haematopoietic commitment event. However, a number of aspects of this lineage restriction process remain poorly understood. Herein this work identified a lympho-myeloid restricted progenitor in the embryo, which resembles the adult LMPP, and demonstrated that lymphoid lineage restriction is initiated prior to definitive haematopoiesis, much earlier than previously appreciated. In vivo fate mapping showed that lympho-myeloid progenitors significantly co

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14

Chang, Chao-Hui. "Haematopoietic stem/progenitor cell interactions with the bone marrow vascular niche." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:452da334-bd4e-45c7-a7bd-fc8767d1239c.

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Umbilical cord blood (UCB) is used as a source of haematopoietic stem cells (HSCs) for transplantation but shows defective homing to the bone marrow niche and delayed haematological reconstitution. Following transplantation, HSCs will home to the bone marrow in response to the CXCL12 chemokine, adhere to the bone marrow sinusoidal endothelial cells and then migrate into and lodge in bone marrow niches. In addition to CXCR4, a variety of molecules have been described as being important in these processes. In this laboratory, junctional adhesion molecule-A (JAM-A) was shown to be expressed on hu

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15

Dalby, Amanda Louise. "Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population : an in vitro system for the production of platelets and red blood cells for transfusion medicine." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/273749.

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There exists a need to produce platelets in vitro for use in transfusion medicine, due to increased platelet demands and short shelf life. Our lab uses human induced pluripotent stem cells (iPSCs), as an attractive alternative supply, as iPSCs can be cultured indefinitely and differentiate into almost any cell type. Using a technique called forward programming, we over express three key haematological transcription factors (TFs), pushing iPSCs towards the megakaryocyte lineage, to produce mature megakaryocytes, the platelet precursor cell type. A major limitation of the forward programming tec

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16

Stoll, Brian R. (Brian Richard) 1973. "A quantitative analysis of the development and remodeling of blood vessels in tumors : contribution of endothelial progenitor cells to angiogenesis and effect of solid stress on blood vessel morphology." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/29606.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2003.<br>Includes bibliographical references (leaves 107-118).<br>Angiogenesis plays a key role in tumor growth. The dependency of tumors on angiogenesis has rendered it a promising therapeutic target. However, to date, this promise has gone unfulfilled in the clinic, suggesting that the current understanding of angiogenesis is insufficient. The objective of this dissertation is to quantitatively analyze the effects of systemic biochemical and cellular contributions as well as local mechanical influences on

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17

Varagnolo, Linda [Verfasser], and Albrecht Manfred [Gutachter] Müller. "PRC2 inhibition counteracts the culture-associated loss of engraftment potential of human cord blood-derived hematopoietic stem/progenitor cells / Linda Varagnolo. Gutachter: Albrecht Manfred Müller." Würzburg : Universität Würzburg, 2015. http://d-nb.info/1111560021/34.

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18

Meissner-Roloff, Madelein. "Prerequisites for establishing a public human UCB SCB; assessment of public acceptance and resistance of UCB to HIV." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/24166.

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South Africa is in dire need of a public umbilical cord blood stem cell bank (UCB SCB). A severe shortage of genetically compatible samples for BM transplantation precludes the majority of South Africans from receiving the relevant medical care. UCB is a viable alternative to BM but is currently disposed of post-delivery. UCB could furthermore serve as a resource of genetically compatible haematopoietic progenitor cells (HPCs) that could be used in gene therapy approaches directed towards a cure for HIV-1. Knowing whether HIV-1 affects or infects primitive HPCs is vital to determine the course

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19

Duarte, Sara. "The role of Notch and GATA3 in postnatal and adult haematopoiesis." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:e4f84964-b135-48a2-be01-551a4cef2b0d.

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The role of Notch in cell fate determination and lineage restriction in the bone marrow (BM) is controversial in the field. Recent studies have convincingly shown that Notch is dispensable for haematopoietic stem cell (HSC) regulation in adult haematopoiesis (Maillard et al., 2008). In contrast, Notch signaling has been proposed to be of importance in the regulation of BM megakaryocyte progenitor differentiation, based on dominant negative genetic approaches, identifying a potentially distinct role for Notch in adult BM haematopoiesis (Mercher et al., 2008). Here, I found that by selectively a

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20

Alsheikh, Manal. "Impact of the Maturation Status of Osteoblasts on Their Hematopoietic Regulatory Activity." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/35899.

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Osteoblasts (OST) provide strong intrinsic growth modulatory activities on hematopoietic stem and progenitor cells via different mechanisms that include secretion of growth factors, and cellular interaction. Previously we showed that medium conditioned by mesenchymal stromal cell (MSC)-derived osteoblasts (M-OST) improve the expansion of cord blood (CB) CD34+ cells. I hypothesize that the hematopoietic supporting activity of M-OST would vary as a function of their maturation. This was tested by producing osteoblast conditioned media (OCM) from M-OST at distinct stages of maturation, and testin

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21

Pepperell, Emma E. "The regulation of stem cell engraftment." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:873a14b9-6c7b-4643-bb34-4e1679d4f734.

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The engraftment of haemopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) into adult recipients, although advantageous in terms of sourcing units, the decreased need to match donor and recipient and reduced risk of graft versus host disease (GvHD), is delayed compared to grafts using HSPCs from mobilised peripheral blood (MPB) or bone marrow (BM). One reason for this is the limited number of HSPCs (CD34+/CD133+ cells) in a unit of UCB compared to MPB or BM. The CXCR4-CXCL12 axis is widely recognised as a key player in the bone marrow homing, retention, and engraftment of HS

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22

Rocha, Helena Naly Miguens. "Apoptose e prejuízo na capacidade de reparo endotelial induzidos por fluxo sanguíneo retrógrado na hipertensão." Niterói, 2017. https://app.uff.br/riuff/handle/1/3787.

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Submitted by Biblioteca do Instituto Biomédico BIB (uffbib@gmail.com) on 2017-06-05T19:47:11Z No. of bitstreams: 1 Helena Naly Miguens Rocha.pdf: 1130470 bytes, checksum: 43146823dc28af52b34aa41dd863ff95 (MD5)<br>Made available in DSpace on 2017-06-05T19:47:11Z (GMT). No. of bitstreams: 1 Helena Naly Miguens Rocha.pdf: 1130470 bytes, checksum: 43146823dc28af52b34aa41dd863ff95 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Mecanismos de ativação e reparo endoteliais em resposta ao fluxo sanguíneo retrógrado (FSR) exacerbado ainda não foram completamente elucidados, nem

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23

Forte, Andresa. "Expansão ex vivo das células-tronco hematopoiéticas do sangue do cordão umbilical: análise comparativa da proliferação celular em cocultura de células-troco mesenquimais provenientes do endotélio vascular do cordão umbilical e do tecido adiposo." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5167/tde-25022015-085731/.

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INTRODUÇÃO: As células-tronco hematopoiéticas (CTH) do sangue do cordão umbilical (SCU) têm sido utilizadas com sucesso para o tratamento de doenças malignas e não malignas. No entanto, algumas unidades de SCU podem apresentar baixa quantidade de células nucleadas totais (CNT). Algumas abordagens têm sido sugeridas para evitar problemas em relação à baixa concentração de CTH no transplante, como a administração de duas unidades de SCU para o paciente e a expansão ex vivo de CTH. OBJETIVO: Avaliar as taxas de proliferação celular na expansão ex vivo do SCU em sistema de cocultura com células-tr

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24

Willis, Fenella Mai. "Optimising peripheral blood progenitor cell mobilisation." Thesis, St George's, University of London, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511964.

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25

Samitsch, Marina. "Dissecting human haematopoietic progenitors." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:58511f8d-cb36-4acf-b706-c465c50f5404.

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Human haematopoiesis resembles a complex hierarchy, however most intermediate stages are only poorly defined. Efforts to characterise human progenitors have been inconsistent and failed to integrate previous knowledge. Furthermore, characterisation of normal progenitors has important implications in acute myeloid leukaemia (AML) biology. We previously established that leukaemic stem cells (LSCs) resemble the immunophenotypic progenitor compartments more closely than the stem cell fraction. Therefore, I set out to characterise human stem and progenitor cells (HSCPs) on phenotypic, molecular and

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26

Pereira, Jessica. "Effets d'un polysaccharide sulfaté, le fucoïdane, sur la réparation osseuse induite par les cellules souches mésenchymateuses." Thesis, Paris 5, 2013. http://www.theses.fr/2013PA05S010.

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Dans le cas de larges pertes de substance osseuse, l’ingénierie tissulaire représente une alternative intéressante aux greffes. Cette technique consiste à associer des cellules à des biomatériaux dans le but de réparer le tissu. L'objectif de ce travail est l'étude de l'amélioration du potentiel ostéogénique des cellules souches mésenchymateuses issues du tissue adipeux humain (ASC), afin d’augmenter la formation de matrice osseuse en territoire ischémique. Nous avons montré que le fucoïdane, un polysaccharide d’origine marine, était capable d’améliorer la différenciation ostéogénique des ASC

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27

Humphreys, Alison C. "Clinical and laboratory studies of high dose chemotherapy and peripheral blood progenitor cell transplantation in breast cancer." Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323491.

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28

Hovey, Owen. "Characterization of Proteins Released by Osteoblasts That Promote Expansion of Hematopoietic Progenitors." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38012.

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Umbilical cord blood (UCB) is a source of hematopoietic stem and progenitor cells (HSPC) used for allogeneic transplantation. Ex vivo expansion of HSPC can improve the slow platelet and neutrophil engraftment associated with UCB transplants. HSPCs reside in niches, some of which are near the endosteal bone surface, where they can associate with immature osteoblasts. Interestingly, osteoblasts can enhance the growth of HSPC in culture and their platelet engraftment activity. Using a proteomics approach, I identified 47 differentially expressed proteins between mesenchymal stem cells and immatur

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29

Diedrich, Beatrice. "Storage and transfusion of platelets in vitro and in vivo studies in healthy volunteers and in allogeneic hematopoetic progenitor cell transplant recipients /." Stockholm : Karolinska institutet, 2009. http://diss.kib.ki.se/2009/978-91-7409-280-6/.

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30

Osma, Córdoba María del Mar. "Estudio de los progenitores hematopoyéticos en el trasplante autogénico en pacientes con carcinoma de mama. Study of the hematopoietic progenitors in patients with breast cancer undergoing autologous peripheral blood stem cell transplantation." Doctoral thesis, Universidad de Murcia, 2000. http://hdl.handle.net/10803/96058.

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En el marco del trasplante autólogo de progenitores de sangre periférica (TASPE), cincuenta pacientes diagnosticadas de cáncer de mama fueron incluidas en un estudio prospectivo en el que se evaluaba el contenido basal de progenitores CD34+/ CD71- en médula ósea (M.O.), como parámetro predictivo de la movilización de células CD34 tras G-CSF. Además, se estudió el papel del G-CSF, tanto a corto plazo como en la regeneración de progenitores medulares al año del procedimiento. El G-CSF post-TASPE demostró acelerar la recuperación de neutrófilos, pero no hubo diferencias significativas en la recu

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31

Decatris, Marios Petrou. "A comparative quantitative study of human myeloid dendritic cell progenitors in cord blood, bone marrow, peripheral blood and their mobilization kinetics in the peripheral blood of cancer patients undergoing leucaphaeresis." Thesis, University of Leicester, 2003. http://hdl.handle.net/2381/29456.

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Dendritic cell (DC)-based immunotherapy has potential for use in the treatment of cancer, infections and transplantation. Generating large numbers of DC from haemopoietic progenitor cells (HPC) is a key step in this process, often achieved by the aphaeresis of HPC, following their mobilization from the bone marrow into peripheral blood (PB) with chemotherapy and growth factor support (usually, granulocyte-colony stimulating factor, G-CSF). The objective of this work was to identify the optimum time for leucaphaeresis of DC progenitors. An established clonogenic assay specific for colony-formin

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32

Zanelli, Ana Paula Rocha Diniz. "Avaliação da viabilidade financeira do banco do sangue de cordão umbilical do Hemocentro de Ribeirão Preto." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/17/17155/tde-07062017-132339/.

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Introdução. As células progenitoras hematopoéticas (CPH) têm sido utilizadas no tratamento de algumas doenças malignas hematológicas e de desordens hematopoéticas. Dentre estas células estão as CPHs provenientes de cordão umbilical e placentário (SCUP). Para coleta e armazenamento destas células foram criados os Bancos de Sangue de Cordão Umbilical e Placentário (BSCUP). No Brasil existe a rede BrasilCord e o BSCUP do Hemocentro de Ribeirão Preto é um dos que compõem esta rede. Objetivo. Avaliar a viabilidade financeira de um banco de sangue de cordão umbilical e placentário público comparando

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33

Fultz, Caroline Brigitte. "Differential Expression of Homing-Associated Cell Adhesion Molecule, Very Late Antigen-4 and L-Selectin in Hematopoietic Progenitor Cell Trafficking between the Marrow and Blood." Scholar Commons, 1998. http://scholarcommons.usf.edu/etd/4427.

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This study addresses the hypothesis that the following cell adhesion molecules (CAMs): homing-associated cell adhesion molecule (HCAM), very late antigen-4 (VLA-4) and L-selecti I I . . n p ay a ro e m the trafficking of hematopoietic progenitor cells (HPCs) between the bone marrow microenvironment and the peripheral circulation. In order to ascertain differences in CAM expression based on physiologic compartment, the expression of HCAM, VLA-4 or L-selectin per CD34+ myeloid progenitor cell was assessed between paired samples of blood and marrow. CAM expression was flow cytometrically quantita

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Schiavinato, Josiane Lilian dos Santos. "Papel de Notch e NF-kB na regulação de fatores de transcrição durante a diferenciação in vitro de células T a partir de células progenitoras hematopoéticas CD34+." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-30102014-115459/.

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Em estudos anteriores desenvolvidos por este grupo de pesquisa uma expressão mais elevada de alvos transcricionais e componentes da via NF-kB, bem como altos níveis de NOTCH1, foi identificada em células-tronco hematopoéticas (CTH) CD34+ de sangue de cordão umbilical (SCU) quando comparadas às CTH CD34+ de medula óssea (MO). Este grupo verificou ainda, por comparação das células CD34+ com as CD133+ (mais primitivas) que diversos fatores de transcrição (FT) envolvidos com o potencial de hemangioblasto, com a autorenovação das CTH, e com a diferenciação linfóide; como: RUNX1/AML1, GATA3, USF1, T

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35

Passos, Paula Renata Machado. "Associação entre os níveis citoplasmáticos da enzima aldeído desidrogenase (ALDH) e a capacidade proliferativa \"in vitro\" das células progenitoras hematopoéticas de sangue de cordão umbilical e placentário." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/17/17155/tde-13092018-160308/.

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A utilização das células progenitoras hematopoéticas (CPH) obtidas do sangue de cordão umbilical e placentário (SCUP) apresenta vários benefícios para o transplante de CPH em comparação às células provenientes de outras fontes. Dentre eles, a maior disponibilidade e a maior imaturidade imunológica das CPH, o que permite certa flexibilidade nos critérios de compatibilidade entre doador e receptor e uma menor taxa de reação do enxerto-versus-hospedeiro. A legislação brasileira e órgãos internacionais exigem a realização de vários testes para garantir a qualidade do produto hemoterápico contendo

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"Hematopoietic stem and progenitor cells in human neonatal blood." 1999. http://library.cuhk.edu.hk/record=b5889999.

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Yau Fung-wan.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 1999.<br>Includes bibliographical references (leaves 147-183).<br>Abstracts in English and Chinese.<br>Acknowledgements --- p.iii<br>Publications --- p.iv<br>Abbreviations --- p.vii<br>Appendix Some cell surface antigens expressed on hematopoietic cells --- p.ix<br>Abstract --- p.x<br>Chapter Chapter One --- Introduction --- p.1<br>Chapter Section A --- Sources of blood stem cells for transplantation --- p.1<br>Chapter Section B --- Hematopoiesis --- p.7<br>Chapter Section C --- Human CD34+ blood cells --- p.15<br>Cha

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Pinho, Maria João Nascimento de. "In vitro determination and functional maturation of natural killer cells from umbilical cord blood progenitor cells." Tese, 2012. https://repositorio-aberto.up.pt/handle/10216/70579.

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Pinho, Maria João Nascimento de. "In vitro determination and functional maturation of natural killer cells from umbilical cord blood progenitor cells." Doctoral thesis, 2012. https://repositorio-aberto.up.pt/handle/10216/70579.

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Rahman, Muhammad Nafeesur. "Vascular Endothelial Growth Factor Functionalized Agarose Can Efficiently Guide Pluripotent Stem Cell Aggregates Toward Blood Progenitor Cells." Thesis, 2010. http://hdl.handle.net/1807/24626.

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Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the embryo that have great potential for regenerative therapies because of their ability to self-renew and differentiate into almost all cell types. However, this developmental potential is influenced by the local cellular microenvironment, including cell surface bound ligands. In this study, we synthesized an artificial stem cell niche wherein vascular endothelial growth factor A (VEGFA) was functionally immobilized in an agarose hydrogel. Immobilized VEGFA treatments were able to upregulate mesodermal markers, brachyur

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Wang, Chao-Hung, and 王兆弘. "Biological Function and Identification of Peripheral Blood-derived Vascular Progenitor Cells Related to Atherosclerosis." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/09761871761936331405.

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博士<br>國立陽明大學<br>臨床醫學研究所<br>100<br>Endothelial colony-forming cells (ECFCs) are undergoing extensive investigations to tackle certain deliberating cardiovascular diseases. However, the success of this approach depends on a thorough understanding of ECFC biology. This study sought to determine the factors associated the purity, biological function and activation potential of ex vivo expanded ECFCs. Seventy-three patients with newly diagnosed coronary artery disease (CAD) and 24 controls were studied. ECFCs were cultured for up to 10 passages to investigate changes in and the impact of coronary r

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Lin, Yi-Huey, and 林怡慧. "The Differentiation Characteristics of Human Adult Peripheral Blood Hematopoietic Stem/ Progenitor Cells in Erythropoiesis." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/81892760216997906890.

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碩士<br>臺北醫學大學<br>細胞及分子生物研究所<br>92<br>Erythropoiesis is a multistep process of the pluripotent hematopoietic stem cells differentiate to the mature red blood cells, which is influenced by extrinsic and intracellular environmental elements. However, the molecular regulation mechanism remains to clarify. In this study, we applied an two-stage in vitro erythropoiesis culture system to probe the cytokine effect, such as EPO and SCF, on adult peripheral blood CD34＋ hematopoietic stem/ progenitor cells. The culture system produced enriched erythroid progenitors and allowing us to evaluate the differen

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Stroncek, John. "Use of Human Blood-Derived Endothelial Progenitor Cells to Improve the Performance of Vascular Grafts." Diss., 2011. http://hdl.handle.net/10161/3849.

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<p>Synthetic small diameter vascular grafts fail clinically due to thrombosis and intimal hyperplasia. The attachment of endothelial cells (ECs) onto the inner lumen of synthetic small diameter vascular grafts can improve graft patency; however, significant challenges remain that prevent wide clinical adoption. These issues include difficulties in the autologous sourcing of ECs, the lack of attachment, growth and retention of the layer of ECs to the graft lumen, and the maintenance of an anti-thrombotic and anti-inflammatory profile by the layer of ECs. </p><p>This dissertation describes t

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Brown, Peters Erica Cho. "A Tissue-Engineered Microvascular System to Evaluate Vascular Progenitor Cells for Angiogenic Therapies." Diss., 2015. http://hdl.handle.net/10161/10493.

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<p>The ability of tissue engineered constructs to replace diseased or damaged organs is limited without the incorporation of a functional vascular system. To design microvasculature that recapitulates the vascular niche functions for each tissue in the body, we investigated the following hypotheses: (1) cocultures of human umbilical cord blood-derived endothelial progenitor cells (hCB-EPCs) with mural cells can produce the microenvironmental cues necessary to support physiological microvessel formation in vitro; (2) poly(ethylene glycol) (PEG) hydrogel systems can support 3D microvessel form

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Chen, Wan-Yu, and 陳婉瑜. "Isolation and characterization of human peripheral blood derived endothelial progenitor-like cells for neo-vasculogenesis." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/01162488774077827884.

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碩士<br>臺北醫學大學<br>細胞及分子生物研究所<br>93<br>This study describes the generation and characterization of human adult peripheral blood mononuclear-derived endothelial progenitor-like cells (EPLCs) as a resource may potentially useful for neo-vasculogenesis. Peripheral blood mononuclear cell derived EPLCs are characterized by their phonotypical expression of CD34–, CD105+, CD14++, CD31++, CD45+++, and VEGF receptors positive (Flt-1++ and KDR++). These cells up-taking AC-LDL, binding ULEX-1 functions and exhibit tube formation in the Matrigel culture system in resemble to the CD34+, KDR++, AC133+ endoth

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Brown, Melissa Ann. "Umbilical Cord Blood Derived Endothelial Progenitor Cells: Isolation, Characterization, and Adhesion Potential in Vitro and in Vivo." Diss., 2009. http://hdl.handle.net/10161/1355.

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<p>The number one cause of death in the industrialized world, atherosclerosis, can be treated through a variety of methods: angioplasty, stenting, vein graft bypass, synthetic grafts, and maybe one day tissue engineering vessels (TEBVs). The long term goal that motivated this research is the delivery of umbilical cord blood derived endothelial progenitor cells (CB-EPCs) to damaged arteries and possibly reducing the rate of re-occlusion by re-establishing a healthy, functional, intact endothelium. The proposed research tested the following hypotheses: (1) Mild trypsinization methods produces s

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Lin, Chang-Zhi, and 林昶志. "Co-culture of Cord Blood-Derived Endothelial Progenitor Cells and Mesenchymal Stem Cells on Electrospun Polyhydroxyalkanoate Scaffold for Vascular Tissue Engineering." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/68913201879738222126.

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碩士<br>元智大學<br>化學工程與材料科學學系<br>104<br>Currently, repair and regeneration of tissue by engineered vascular tissue is an important research topic for biomedical engineering and regenerative medicine. Many studies indicated that growth of tissue not only depended on cell, scaffold and signal but also depended on vascular to supply nutrients and oxygen. In addition, many studies also demonstrated that mesenchymal stem cells (MSCs) can support and enhance endothelial progenitor cells (EPCs) to form angiogenesis. The aim of this study was to co-culture cord blood derived EPCs and MSCs on the 2-D surfa

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Shirvaikar, Neeta Chandan. "Role of membrane-type 1 matrix metalloproteinase in hematopoietic stem/progenitor cell trafficking." Phd thesis, 2010. http://hdl.handle.net/10048/1146.

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Thesis (Ph.D.)--University of Alberta, 2010.<br>A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Medicine. Title from pdf file main screen (viewed on April 27, 2010). Includes bibliographical references.

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"Ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood cytokine combinations, platelet-derived growth factor and stromal cell support." 2002. http://library.cuhk.edu.hk/record=b6073426.

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"February 2002."<br>Thesis (Ph.D.)--Chinese University of Hong Kong, 2002.<br>Includes bibliographical references (p. 171-209).<br>Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.<br>Mode of access: World Wide Web.<br>Abstracts in English and Chinese.

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"Effects of growth factors and media on the ex vivo expansion of cord blood hematopoietic stem and progenitor cells for transplantation." 2001. http://library.cuhk.edu.hk/record=b5890896.

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Lam Audrey Carmen.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2001.<br>Includes bibliographical references (leaves 166-195).<br>Abstracts in English and Chinese.<br>Acknowledgements --- p.vi<br>Publications --- p.vii<br>Abbreviations --- p.x<br>Abstract --- p.xiii<br>Chapter Chapter One - --- Introduction --- p.1<br>Chapter Section 1.1 --- Hematopoietic Stem Cells --- p.1<br>Chapter 1.1.1 --- Hematopoiesis --- p.1<br>Chapter 1.1.2 --- Hematopoietic Stem and Progenitor Cells --- p.1<br>Chapter Section 1.2 --- Stem Cell Transplantation --- p.4<br>Chapter 1.2.1 --- Stem Cell T

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Varagnolo, Linda. "PRC2 inhibition counteracts the culture-associated loss of engraftment potential of human cord blood-derived hematopoietic stem/progenitor cells." Doctoral thesis, 2014. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-108073.

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Cord blood hematopoietic stem cells (CB-HSCs) are an outstanding source for the treatment of a variety of malignant and non-malignant disorders. However, the low amount of cells collected per donor is often insufficient for treatment of adult patients. In order to make sufficient numbers of CB-HSCs available for adults, expansion is required. Different approaches were described for HSC expansion, however these approaches are impeded by the loss of engrafting potential during ex vivo culture. Little is known about the underlying molecular mechanisms. Epigenetic mechanisms play essential roles i

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