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Artigos de revistas sobre o tema "DNA Synthesis"

Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 25 de julho de 2025

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1

Shaista Sabir and Naghmmana Rashid, Shaista Sabir and Naghmmana Rashid. "Organocatalyzed Synthesis, DNA Binding and Microbial Studies of Warfarin Analogues." Journal of the chemical society of pakistan 46, no. 1 (2024): 107. http://dx.doi.org/10.52568/001426/jcsp/46.01.2024.

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A noval chiral sec-amine/amidine-base hybrid catalyst, N [S-carbonylprolyl] cyclohexyl Amine is described, which is able to catalyze conjugate addition of 6-Methyl-4-hydroxypyran and 2-Hydroxy-naphthaquinone with various benzylideneacetones through Michael reactions that directly gives anticoagulant Warfarin analogues. These analogues were prepared in good yields (54–82%) and in good enantiomeric excess (50–75%). Identification of synthesized compounds was done by physio-chemical properties and spectral analysis (1H-NMR andamp; 13C-NMR).These compounds were further investigated for their antim

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2

Shilkin, E. S., E. O. Boldinova, A. D. Stolyarenko, et al. "Translesion DNA Synthesis and Reinitiation of DNA Synthesis in Chemotherapy Resistance." Biochemistry (Moscow) 85, no. 8 (2020): 869–82. http://dx.doi.org/10.1134/s0006297920080039.

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3

Burke, Cassandra R., and Andrej Lupták. "DNA synthesis from diphosphate substrates by DNA polymerases." Proceedings of the National Academy of Sciences 115, no. 5 (2018): 980–85. http://dx.doi.org/10.1073/pnas.1712193115.

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The activity of DNA polymerase underlies numerous biotechnologies, cell division, and therapeutics, yet the enzyme remains incompletely understood. We demonstrate that both thermostable and mesophilic DNA polymerases readily utilize deoxyribonucleoside diphosphates (dNDPs) for DNA synthesis and inorganic phosphate for the reverse reaction, that is, phosphorolysis of DNA. For Taq DNA polymerase, the KMs of the dNDP and phosphate substrates are ∼20 and 200 times higher than for dNTP and pyrophosphate, respectively. DNA synthesis from dNDPs is about 17 times slower than from dNTPs, and DNA phosph

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4

Caruthers, Marvin H. "Chemical synthesis of DNA and DNA analogs." Accounts of Chemical Research 24, no. 9 (1991): 278–84. http://dx.doi.org/10.1021/ar00009a005.

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5

Church, Geoffrey A., Anindya Dasgupta, and Duncan W. Wilson. "Herpes Simplex Virus DNA Packaging without Measurable DNA Synthesis." Journal of Virology 72, no. 4 (1998): 2745–51. http://dx.doi.org/10.1128/jvi.72.4.2745-2751.1998.

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ABSTRACT Herpes simplex virus (HSV) type 1 DNA synthesis and packaging occur within the nuclei of infected cells; however, the extent to which the two processes are coupled remains unclear. Correct packaging is thought to be dependent upon DNA debranching or other repair processes, and such events commonly involve new DNA synthesis. Furthermore, the HSV UL15 gene product, essential for packaging, nevertheless localizes to sites of active DNA replication and may link the two events. It has previously been difficult to determine whether packaging requires concomitant DNA synthesis due to the com

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6

Sariban, E., R. S. Wu, L. C. Erickson, and W. M. Bonner. "Interrelationships of protein and DNA syntheses during replication of mammalian cells." Molecular and Cellular Biology 5, no. 6 (1985): 1279–86. http://dx.doi.org/10.1128/mcb.5.6.1279-1286.1985.

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During the replication of chromatin, the syntheses of the histone protein and DNA components are closely coordinated but not totally linked. The interrelationships of total protein synthesis, histone protein synthesis, DNA synthesis, and mRNA levels have been investigated in Chinese hamster ovary cells subjected to several different types of inhibitors in several different temporal combinations. The results from these studies and results reported elsewhere can be brought together into a consistent framework which combines the idea of autoregulation of histone biosynthesis as originally propose

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7

Sariban, E., R. S. Wu, L. C. Erickson, and W. M. Bonner. "Interrelationships of protein and DNA syntheses during replication of mammalian cells." Molecular and Cellular Biology 5, no. 6 (1985): 1279–86. http://dx.doi.org/10.1128/mcb.5.6.1279.

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During the replication of chromatin, the syntheses of the histone protein and DNA components are closely coordinated but not totally linked. The interrelationships of total protein synthesis, histone protein synthesis, DNA synthesis, and mRNA levels have been investigated in Chinese hamster ovary cells subjected to several different types of inhibitors in several different temporal combinations. The results from these studies and results reported elsewhere can be brought together into a consistent framework which combines the idea of autoregulation of histone biosynthesis as originally propose

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8

Turgay Tun, Turgay Tun, Nadir Demirel Nadir Demirel, Mahmut Emir Mahmut Emir, Asl han G. nel Asl han G nel, R. fk Kad o. lu R fk Kad o lu, and Nurcan Karacan Nurcan Karacan. "Three New Copper (II) Complexes with CHIRAL SCHIFF BASES: Synthesis, Characterization, DNA Binding and DNA-Cleavage Studies." Journal of the chemical society of pakistan 41, no. 2 (2019): 334. http://dx.doi.org/10.52568/000730/jcsp/41.02.2019.

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New mononuclear copper (II) complexes (1, 2 and 3) were synthesized from Schiff bases (H2L) of chiral amino alcohols. The structures of the copper complexes were proposed by a combination of elemental analyses, FTIR, LCMS, magnetic susceptibility and molar conductance measurement methods. Spectroscopic and analytical data of the complexes suggest four-coordinated structures. Geometry optimization carried out with DFT/6-31G (d,p) were proposed to be distorted square planar geometry for the complexes. The similarity between experimental and theoretical IR spectra confirms the proposed structures

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9

LUCK, DENNIS N., JOHNNY K. NGSEE, FRITZ M. ROTTMAN, and MICHAEL SMITH. "Synthesis of Bovine Prolactin inEscherichia coli." DNA 5, no. 1 (1986): 21–28. http://dx.doi.org/10.1089/dna.1986.5.21.

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10

Leslie, Mitch. "Double-checking DNA synthesis." Journal of Cell Biology 204, no. 2 (2014): 148. http://dx.doi.org/10.1083/jcb.2042iti1.

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11

Doerr, Allison. "DNA synthesis lights up." Nature Methods 5, no. 4 (2008): 286. http://dx.doi.org/10.1038/nmeth0408-286.

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12

Uppenbrink, J. "ORGANIC SYNTHESIS: Sugarcoated DNA." Science 290, no. 5492 (2000): 675b—675. http://dx.doi.org/10.1126/science.290.5492.675b.

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13

LeBrasseur, Nicole. "Geminin halts DNA synthesis." Journal of Cell Biology 165, no. 4 (2004): 455. http://dx.doi.org/10.1083/jcb1654iti3.

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14

MILLS, W. RONALD, MICHELE REEVES, DIANA L. FOWLER, and STEPHEN F. CAPO. "DNA Synthesis in Chloroplasts." Journal of Experimental Botany 40, no. 4 (1989): 425–29. http://dx.doi.org/10.1093/jxb/40.4.425.

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15

Caruthers, Marvin H. "Chemical synthesis of DNA." Journal of Chemical Education 66, no. 7 (1989): 577. http://dx.doi.org/10.1021/ed066p577.

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16

HENRY, CELIA M. "DNA-PROGRAMMED ORGANIC SYNTHESIS." Chemical & Engineering News Archive 83, no. 5 (2005): 35–36. http://dx.doi.org/10.1021/cen-v083n005.p035.

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17

Leake, Devin. "DNA Synthesis Steps Up." Genetic Engineering & Biotechnology News 36, no. 8 (2016): 14–15. http://dx.doi.org/10.1089/gen.36.08.09.

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18

Wright, N. A. "DNA Synthesis and Genotoxicity." Digestion 47, no. 1 (1990): 24–30. http://dx.doi.org/10.1159/000200511.

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19

Hansen, Bjarke N., Kim S. Larsen, Daniel Merkle, and Alexei Mihalchuk. "DNA-templated synthesis optimization." Natural Computing 17, no. 4 (2018): 693–707. http://dx.doi.org/10.1007/s11047-018-9697-7.

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20

HAYDEN, MARK A., and WLODEK MANDECKI. "Gene Synthesis by Serial Cloning of Oligonucleotides." DNA 7, no. 8 (1988): 571–77. http://dx.doi.org/10.1089/dna.1.1988.7.571.

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21

EBE, KAZUYU, MONICA SCHÖLD, JOHN J. ROSSI, and R. BRUCE WALLACE. "Enzymatic Synthesis of Oligoribonucleotides of Defined Sequence." DNA 6, no. 5 (1987): 497–504. http://dx.doi.org/10.1089/dna.1987.6.497.

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22

Hogg, Matthew, A. Elisabeth Sauer-Eriksson та Erik Johansson. "Promiscuous DNA synthesis by human DNA polymerase θ". Nucleic Acids Research 40, № 6 (2011): 2611–22. http://dx.doi.org/10.1093/nar/gkr1102.

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23

Bridges, Bryn A. "Error-prone DNA repair and translesion DNA synthesis." DNA Repair 4, no. 6 (2005): 725–39. http://dx.doi.org/10.1016/j.dnarep.2004.12.009.

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24

Ebara, Y., T. Sugiyama, K. Kaihatsu, and S. i. Ueji. "Synthesis of non-natural DNA using DNA polymerase." Nucleic Acids Symposium Series 44, no. 1 (2000): 143–44. http://dx.doi.org/10.1093/nass/44.1.143.

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25

Chary, Parvathi, William A. Beard, Samuel H. Wilson та R. Stephen Lloyd. "DNA Polymerase β Gap-Filling Translesion DNA Synthesis". Chemical Research in Toxicology 25, № 12 (2012): 2744–54. http://dx.doi.org/10.1021/tx300368f.

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26

Zhang, Yanbin, Xiaohua Wu, Fenghua Yuan, Zhongwen Xie та Zhigang Wang. "Highly Frequent Frameshift DNA Synthesis by Human DNA Polymerase μ". Molecular and Cellular Biology 21, № 23 (2001): 7995–8006. http://dx.doi.org/10.1128/mcb.21.23.7995-8006.2001.

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ABSTRACT DNA polymerase μ (Polμ) is a newly identified member of the polymerase X family. The biological function of Polμ is not known, although it has been speculated that human Polμ may be a somatic hypermutation polymerase. To help understand the in vivo function of human Polμ, we have performed in vitro biochemical analyses of the purified polymerase. Unlike any other DNA polymerases studied thus far, human Polμ catalyzed frameshift DNA synthesis with an unprecedentedly high frequency. In the sequence contexts examined, −1 deletion occurred as the predominant DNA synthesis mechanism opposi

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27

Xiong, X., J. L. Smith, and M. S. Chen. "Effect of incorporation of cidofovir into DNA by human cytomegalovirus DNA polymerase on DNA elongation." Antimicrobial Agents and Chemotherapy 41, no. 3 (1997): 594–99. http://dx.doi.org/10.1128/aac.41.3.594.

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Cidofovir (CDV) (HPMPC) has potent in vitro and in vivo activity against human cytomegalovirus (HCMV), CDV diphosphate (CDVpp), the putative antiviral metabolite of CDV, is an inhibitor and an alternate substrate of HCMV DNA polymerase. CDV is incorporated with the correct complementation to dGMP in the template, and the incorporated CDV at the primer end is not excised by the 3'-to-5' exonuclease activity of HCMV DNA polymerase. The incorporation of a CDV molecule causes a decrease in the rate of DNA elongation for the addition of the second natural nucleotide from the singly incorporated CDV

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28

SHIMKUS, MARY L., PERRY GUAGLIANONE, and TIMOTHY M. HERMAN. "Synthesis and Characterization of Biotin-Labeled Nucleotide Analogs." DNA 5, no. 3 (1986): 247–55. http://dx.doi.org/10.1089/dna.1986.5.247.

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29

BODESCOT, MYRIAM, and OLIVIER BRISON. "Efficient Second-Strand cDNA Synthesis Using T7 DNA Polymerase." DNA and Cell Biology 13, no. 9 (1994): 977–85. http://dx.doi.org/10.1089/dna.1994.13.977.

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30

Nasheuer, Heinz Peter, and Anna Marie Meaney. "Starting DNA Synthesis: Initiation Processes during the Replication of Chromosomal DNA in Humans." Genes 15, no. 3 (2024): 360. http://dx.doi.org/10.3390/genes15030360.

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The initiation reactions of DNA synthesis are central processes during human chromosomal DNA replication. They are separated into two main processes: the initiation events at replication origins, the start of the leading strand synthesis for each replicon, and the numerous initiation events taking place during lagging strand DNA synthesis. In addition, a third mechanism is the re-initiation of DNA synthesis after replication fork stalling, which takes place when DNA lesions hinder the progression of DNA synthesis. The initiation of leading strand synthesis at replication origins is regulated a

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31

MacCulloch, Tara, Alexandra Novacek, and Nicholas Stephanopoulos. "Proximity-enhanced synthesis of DNA–peptide–DNA triblock molecules." Chemical Communications 58, no. 25 (2022): 4044–47. http://dx.doi.org/10.1039/d1cc04970d.

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32

Chaput, John C., Justin K. Ichida, and Jack W. Szostak. "DNA Polymerase-Mediated DNA Synthesis on a TNA Template." Journal of the American Chemical Society 125, no. 4 (2003): 856–57. http://dx.doi.org/10.1021/ja028589k.

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33

Arana, M. E., M. Seki, R. D. Wood, I. B. Rogozin, and T. A. Kunkel. "Low-fidelity DNA synthesis by human DNA polymerase theta." Nucleic Acids Research 36, no. 11 (2008): 3847–56. http://dx.doi.org/10.1093/nar/gkn310.

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34

Gomez Godinez, Veronica, Sami Kabbara, Adria Sherman, et al. "DNA damage induced during mitosis undergoes DNA repair synthesis." PLOS ONE 15, no. 4 (2020): e0227849. http://dx.doi.org/10.1371/journal.pone.0227849.

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35

Paz-Elizur, Tamar, Masaru Takeshita, Myron Goodman, Michael O'Donnell, and Zvi Livneh. "Mechanism of Translesion DNA Synthesis by DNA Polymerase II." Journal of Biological Chemistry 271, no. 40 (1996): 24662–69. http://dx.doi.org/10.1074/jbc.271.40.24662.

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36

Lee, S. H., Z. Q. Pan, A. D. Kwong, P. M. Burgers, and J. Hurwitz. "Synthesis of DNA by DNA polymerase epsilon in vitro." Journal of Biological Chemistry 266, no. 33 (1991): 22707–17. http://dx.doi.org/10.1016/s0021-9258(18)54626-3.

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37

Tomiyasu, Shinjiro, Kazuhiko Kuwahara, Nobuo Sakaguchi, and Michio Ogawa. "GANP DNA primase associated with MCM3 and DNA synthesis." International Congress Series 1255 (August 2003): 283–88. http://dx.doi.org/10.1016/s0531-5131(03)00917-8.

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38

Liang, X., T. Kato, and H. Asanuma. "Mechanism of DNA elongation during de novo DNA synthesis." Nucleic Acids Symposium Series 52, no. 1 (2008): 411–12. http://dx.doi.org/10.1093/nass/nrn209.

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39

Barone, G., L. De Napoli, G. Di Fabio, et al. "Synthesis and DNA Binding Properties of DNA-PNA Chimeras." Nucleosides, Nucleotides and Nucleic Acids 22, no. 5-8 (2003): 1089–91. http://dx.doi.org/10.1081/ncn-120022743.

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40

Matsuda, Toshiro, Katarzyna Bebenek, Chikahide Masutani, Fumio Hanaoka та Thomas A. Kunkel. "Low fidelity DNA synthesis by human DNA polymerase-η". Nature 404, № 6781 (2000): 1011–13. http://dx.doi.org/10.1038/35010014.

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41

CARUTHERS, M. H. "ChemInform Abstract: Chemical Synthesis of DNA and DNA Analogues." ChemInform 23, no. 4 (2010): no. http://dx.doi.org/10.1002/chin.199204308.

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42

Lo, Chen-Yu, and Yang Gao. "DNA Polymerase-Parental DNA Interaction Is Essential for Helicase-Polymerase Coupling during Bacteriophage T7 DNA Replication." International Journal of Molecular Sciences 23, no. 3 (2022): 1342. http://dx.doi.org/10.3390/ijms23031342.

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DNA helicase and polymerase work cooperatively at the replication fork to perform leading-strand DNA synthesis. It was believed that the helicase migrates to the forefront of the replication fork where it unwinds the duplex to provide templates for DNA polymerases. However, the molecular basis of the helicase-polymerase coupling is not fully understood. The recently elucidated T7 replisome structure suggests that the helicase and polymerase sandwich parental DNA and each enzyme pulls a daughter strand in opposite directions. Interestingly, the T7 polymerase, but not the helicase, carries the p

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43

Zhang, Hui Yong. "Solid-Phase Synthesis of DNA Chemical Sensor." Advanced Materials Research 815 (October 2013): 305–11. http://dx.doi.org/10.4028/www.scientific.net/amr.815.305.

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Oligonucleotides are essential components of many applications in molecular biology. The synthesis chemistry is robust and commercial oligonucleotide synthesizers have taken advantage of the chemistry to provide oligonucleotides of high quality and purity. This paper established nucleic acid synthesis platform to carry out the synthesis of the labeled nucleic acid probes based on the DNA synthesizer and solid-phase synthesis technology. We chose to study the automated synthesis starting from DMT protected FAM labeled amidite attached to controlled pore glass (CPG) support and the standard trit

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44

Bügl, Hans, John P. Danner, Robert J. Molinari, et al. "DNA synthesis and biological security." Nature Biotechnology 25, no. 6 (2007): 627–29. http://dx.doi.org/10.1038/nbt0607-627.

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45

Shivalingam, Arun, and Tom Brown. "Synthesis of chemically modified DNA." Biochemical Society Transactions 44, no. 3 (2016): 709–15. http://dx.doi.org/10.1042/bst20160051.

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Naturally occurring DNA is encoded by the four nucleobases adenine, cytosine, guanine and thymine. Yet minor chemical modifications to these bases, such as methylation, can significantly alter DNA function, and more drastic changes, such as replacement with unnatural base pairs, could expand its function. In order to realize the full potential of DNA in therapeutic and synthetic biology applications, our ability to ‘write’ long modified DNA in a controlled manner must be improved. This review highlights methods currently used for the synthesis of moderately long chemically modified nucleic aci

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46

Yamashita, Takayuki, Tsukasa Oda, and Takayuki Sekimoto. "Translesion DNA Synthesis and Hsp90." Genes and Environment 34, no. 2 (2012): 89–93. http://dx.doi.org/10.3123/jemsge.34.89.

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47

Pincus, SH, KS Ramesh, and DJ Wyler. "Eosinophils stimulate fibroblast DNA synthesis." Blood 70, no. 2 (1987): 572–74. http://dx.doi.org/10.1182/blood.v70.2.572.572.

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Abstract Fibrosis complicates a number of chronic inflammatory diseases and occurs in some conditions following chronic hypereosinophilic syndromes. We assessed whether eosinophils might be a source of fibrogenic factors. Extracts of human and guinea pig cell populations enriched for eosinophils contained substances that stimulated tritiated thymidine incorporation by human fibroblasts. Supernatants derived from resting eosinophils and extracts prepared from eosinophil granules also contained fibrogenic factors. Our findings demonstrate a new potential role for eosinophils and suggest a causal

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48

Pincus, SH, KS Ramesh, and DJ Wyler. "Eosinophils stimulate fibroblast DNA synthesis." Blood 70, no. 2 (1987): 572–74. http://dx.doi.org/10.1182/blood.v70.2.572.bloodjournal702572.

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Fibrosis complicates a number of chronic inflammatory diseases and occurs in some conditions following chronic hypereosinophilic syndromes. We assessed whether eosinophils might be a source of fibrogenic factors. Extracts of human and guinea pig cell populations enriched for eosinophils contained substances that stimulated tritiated thymidine incorporation by human fibroblasts. Supernatants derived from resting eosinophils and extracts prepared from eosinophil granules also contained fibrogenic factors. Our findings demonstrate a new potential role for eosinophils and suggest a causal relation

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49

HANNIGAN, B. H., K. L. O'NEILL, R. H. PEARCE, P. G. McKENNA, and W. P. ABRAM. "Lymphocyte DNA synthesis in malignancy." Biochemical Society Transactions 14, no. 1 (1986): 81–82. http://dx.doi.org/10.1042/bst0140081.

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50

Bong, Dennis, and Jie Mao. "Synthesis of DNA-Binding Peptoids." Synlett 26, no. 11 (2015): 1581–85. http://dx.doi.org/10.1055/s-0034-1380698.

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