Bibliografias temáticas / Enrichment culture

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Literatura científica selecionada sobre o tema "Enrichment culture"

Autor: Grafiati
Publicado: 4 de junho de 2021
Última modificação: 5 de fevereiro de 2022

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Artigos de revistas sobre o assunto "Enrichment culture"

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Sansupa, Chakriya, Sara Fareed Mohamed Wahdan, Terd Disayathanoowat e Witoon Purahong. "Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures". Biology 10, n.º 7 (22 de junho de 2021): 569. http://dx.doi.org/10.3390/biology10070569.

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This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence similarity threshold), the fact that enrichment cultures could capture both rare and abundant bacterial taxa in forest soil samples was demonstrated. Enrichment culture and eDNA communities shared 2% of OTUs detected in total community, whereas 88% of enrichment cultures community (15% of total community) could not be detected by eDNA. The enrichment culture-based methods observed 17% of the bacteria in total community. FAPROTAX functional prediction showed that the rare and unique taxa, which were detected with the enrichment cultures, have potential to perform important functions in soil systems. We suggest that enrichment culture-based amplicon sequencing could be a beneficial approach to evaluate a cultured bacterial community. Combining this approach together with the eDNA method could provide more comprehensive information of a bacterial community. We expected that more unique cultured taxa could be detected if further studies used both selective and non-selective culture media to enrich bacteria at the first step.
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Conrad, Cheyenne C., Kim Stanford, Tim A. McAllister, James Thomas e Tim Reuter. "Competition during enrichment of pathogenicEscherichia colimay result in culture bias". FACETS 1, n.º 1 (1 de março de 2017): 114–26. http://dx.doi.org/10.1139/facets-2016-0007.

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Deadly outbreaks and illnesses due to Shiga toxin-producing Escherichia coli (STEC) occur worldwide; however, the cultivation methods required for adequate monitoring and traceback investigations are inefficient at best. Detection of STEC relies heavily on enrichment; yet no standard media or protocols exist. Furthermore, whether enrichment may bias detection of multiple STEC serogroups from complex samples is unknown. Thus, 14 STEC strains of serogroups O157 and the top six non-O157s (O26, O45, O103, O111, O121, and O145) were enriched in pairs for 6–78 h in broth and evaluated by quantitative polymerase chain reaction (qPCR). Here we show that a conventional 6-h enrichment protocol did not result in intra-species culture bias for the isolates tested. However, subsequent enrichments often produced biased cultures, with differences in the qPCR gene copy number ≥2 log10apparent in 12%, 38%, and 52% of competitions after 30, 54, and 78 h of consecutive enrichments, respectively. Some strains were able to prevail and (or) out-compete the opponent strain in 100% of competitions. Our results suggest that culture bias should be considered and (or) evaluated further due to the potential implications during routine pathogen screening and outbreak investigations.
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Kamashwaran, S. R., e Don L. Crawford. "Mechanisms of cadmium resistance in anaerobic bacterial enrichments degrading pentachlorophenol". Canadian Journal of Microbiology 49, n.º 7 (1 de julho de 2003): 418–24. http://dx.doi.org/10.1139/w03-053.

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The mechanisms of heavy-metal resistance used by adapted sulfidogenic and methanogenic enrichments degrading pentachlorophenol in the presence of cadmium (Cd) were studied. The enrichment cultures adapted to and readily tolerated bioavailable Cd concentrations up to 50 ppm while degrading an equal concentration of pentachlorophenol. Both cultures removed >95% of the Cd from solution. Transmission electron micrographs revealed (i) the presence of electron-dense particles surrounding the cells in the sulfidogenic enrichments and (ii) the unusual clumping of cells and the presence of an exopolymer in the methanogenic enrichments. Energy dispersive X-ray analysis showed that the sulfidogenic enrichments removed Cd by extracellular precipitation of cadmium sulfide, while the methanogenic enrichment culture removed Cd by extracellular sequestration of Cd into the exopolymer.Key words: cadmium, pentachlorophenol, sulfidogenic, methanogenic, resistance.
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Duvall, Robert E., Marjut Eklund, Tony T. Tran e Anthony D. Hitchins. "Improved DNA Probe Detection of Listeria monocytogenes in Enrichment Culture After Physical-Chemical Fractionation". Journal of AOAC INTERNATIONAL 89, n.º 1 (1 de janeiro de 2006): 172–79. http://dx.doi.org/10.1093/jaoac/89.1.172.

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Abstract Bacterial detection in foods by nucleic acid probes is limited by microflora competition during selective enrichment. Probe target concentration by extraction and fractionation of enrichments may diminish this limitation. The 1-h AccuProbe chemiluminescent culture identification test for Listeria monocytogenes was used as a model. Its high detection threshold provides a stringent challenge for evaluating enrichmentwork-up protocols. Detection of L. monocytogenes, at 14 colony-forming units/g food,was not consistently possible in 48 h enrichment cultures usingAccuProbe. Concentration by cell sedimentationwas occasionally helpful but the volume of co-sedimented food limited concentration to about 10-fold. To improve concentration, enrichment sedimentswere sonicated or enzymatically lysed to release the probe's target, r-RNA. The RNAwas separated from non-RNA material by extraction with phenol and precipitation with ethanol. Enrichments (250 mL) were concentrated 2500-fold, and the limitation was food RNA volume. A strongly competitive Enterococcus faecium food isolate was used to demonstrate the effect of artificial competition on the kit's ability to detect L. monocytogenes in enrichments. High competitor concentrations repressed the level of the target below the detection threshold, but concentration of r-RNA enabled detection of L. monocytogenes. The effectiveness of this enrichment sample work-up was demonstrated with naturally contaminated hummus.
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Grosser, Robert J., Michael Friedrich, David M. Ward e William P. Inskeep. "Effect of Model Sorptive Phases on Phenanthrene Biodegradation: Different Enrichment Conditions Influence Bioavailability and Selection of Phenanthrene-Degrading Isolates". Applied and Environmental Microbiology 66, n.º 7 (1 de julho de 2000): 2695–702. http://dx.doi.org/10.1128/aem.66.7.2695-2702.2000.

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ABSTRACT The sorption of organic contaminants by natural organic matter (NOM) often limits substrate bioavailability and is an important factor affecting microbial degradation rates in soils and sediments. We hypothesized that reduced substrate bioavailability might influence which microbial assemblages are responsible for contaminant degradation under enrichment culture conditions. Our primary goal was to characterize enrichments in which different model organic solid phases were used to establish a range of phenanthrene bioavailabilities for soil microorganisms. Phenanthrene sorption coefficients (expressed as log KD values) ranged from 3.0 liters kg−1 for Amberlite carboxylic acid cation-exchange resin (AMB) to 3.5 liters kg−1 for Biobeads polyacrylic resin (SM7) and 4.2 liters kg−1 for Biobeads divinyl benzene resin (SM2). Enrichment cultures were established for control (no sorptive phase), sand, AMB, SM7, and SM2 treatments by using two contaminated soils (from Dover, Ohio, and Libby, Mont.) as the initial inocula. The effects of sorption by model phases on the degradation of phenanthrene were evaluated for numerous transfers in order to obtain stable microbial assemblages representative of sorptive and nonsorptive enrichment cultures and to eliminate the effects of the NOM present in the initial inoculum. Phenanthrene degradation rates were similar for each soil inoculum and ranged from 4 to 5 μmol day−1 for control and sand treatments to approximately 0.4 μmol day−1 in the presence of the SM7 sorptive phase. The rates of phenanthrene degradation in the highly sorptive SM2 enrichment culture were insignificant; consequently, stable microbial populations could not be obtained. Bacterial isolates obtained from serial dilutions of enrichment culture samples exhibited significant differences in rates of phenanthrene degradation performed in the presence of SM7, suggesting that enrichments performed in the presence of a sorptive phase selected for different microbial assemblages than control treatments containing solid phase phenanthrene.
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D’Aoust, Jean-Yves, Anne M. Sewell e Paula Greco. "Detection of Salmonella in Dry Foods Using Refrigerated Pre-Enrichment and Enrichment Broth Cultures: Summary of Collaborative Study". Journal of AOAC INTERNATIONAL 77, n.º 6 (1 de novembro de 1994): 1490–91. http://dx.doi.org/10.1093/jaoac/77.6.1490.

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Abstract A collaborative study was conducted to compare the productivity of refrigerated pre-enrichment and enrichment broth cultures with the U.S. Food and Drug Administration culture methods for detection of Salmonella. The refrigerated pre-enrichment and selective enrichment broth culture methods for detection of Salmonella in dry foods have been adopted first action by AOAC INTERNATIONAL.
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WILLIAMS, L. K., L. C. SAIT, T. A. COGAN, F. JØRGENSEN, R. GROGONO-THOMAS e T. J. HUMPHREY. "Enrichment culture can bias the isolation ofCampylobactersubtypes". Epidemiology and Infection 140, n.º 7 (19 de setembro de 2011): 1227–35. http://dx.doi.org/10.1017/s0950268811001877.

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SUMMARYEnrichment culture is often used to isolateCampylobacter. This study compared isolation ofCampylobacterspp. from 119 broiler chicken environments from two farms, using Preston and modified Exeter (mExeter) and modified Bolton (mBolton) enrichments. mExeter was significantly more effective in isolatingCampylobacterspp. from the environmental samples compared to Preston (P<0·001) and mBolton (P<0·04) broths but there was no significant difference between the latter two methods (P>0·05). Enrichment broth type did not affect isolation from chicken faecal or soil and litter samples.C. jejuniwas isolated from significantly more environmental samples using mExeter broth compared to Preston (P<0·01) and mBolton (P<0·003) broths; there was no difference between the latter two methods or between all methods for detection ofC. coli(P>0·05). OnlyC. coliwas isolated from the soil and litter samples and although bothC. jejuniandC. coliwere recovered from the faecal samples there was no effect of using different enrichment broths. The majority of samples where the same species had been isolated yielded the same or closely related genotypes as defined by pulsed-field gel electrophoresis. Isolates recovered using Preston and mBolton broths were less genetically diverse than those from mExeter broth. We conclude that the enrichment method used affects both the number and species ofCampylobacterisolated from naturally contaminated samples.
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Hilyard, Edward J., Joanne M. Jones-Meehan, Barry J. Spargo e Russell T. Hill. "Enrichment, Isolation, and Phylogenetic Identification of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria from Elizabeth River Sediments†". Applied and Environmental Microbiology 74, n.º 4 (21 de dezembro de 2007): 1176–82. http://dx.doi.org/10.1128/aem.01518-07.

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ABSTRACT The diversity of indigenous bacteria in sediments from several sites in the Elizabeth River (Virginia) able to degrade multiple polycyclic aromatic hydrocarbons (PAHs) was investigated by the use of classical selective enrichment and molecular analyses. Enrichment cultures containing naphthalene, phenanthrene, fluoranthene, or pyrene as a sole carbon and energy source were monitored by denaturing gradient gel electrophoresis (DGGE) to detect changes in the bacterial-community profile during enrichment and to determine whether the representative strains present were successfully cultured. The DGGE profiles of the final enrichments grown solely on naphthalene and pyrene showed no clear relationship with the site from which the inoculum was obtained. The enrichments grown solely on pyrene for two sample sites had >80% similarity, which suggests that common pyrene-degrading strains may be present in these sediments. The final enrichments grown on fluoranthene and phenanthrene remained diverse by site, suggesting that these strains may be influenced by environmental conditions. One hundred and one isolates were obtained, comprising representatives of the actinomycetes and alpha-, beta-, and gammaproteobacteria, including seven novel isolates with 16S rRNA gene sequences less than 98% similar to known strains. The ability to degrade multiple PAHs was demonstrated by mineralization of 14C-labeled substrate and growth in pure culture. This supports our hypothesis that a high diversity of bacterial strains with the ability to degrade multiple PAHs can be confirmed by the combined use of classical selective enrichment and molecular analyses. This large collection of diverse PAH-degrading strains provides a valuable resource for studies on mechanisms of PAH degradation and bioremediation.
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MURAKAMI, TAKU. "Filter-Based Pathogen Enrichment Technology for Detection of Multiple Viable Foodborne Pathogens in 1 Day". Journal of Food Protection 75, n.º 9 (1 de setembro de 2012): 1603–10. http://dx.doi.org/10.4315/0362-028x.jfp-12-039.

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Conventional foodborne pathogen assays currently used in the food industry often require long culture enrichments to increase pathogen levels so they can be detected. Even using sensitive real-time PCR assays, culture enrichment at least overnight is necessary especially for detection of pathogens with slow growth rates such as Listeria monocytogenes. To eliminate this cumbersome enrichment step and detect minute amounts of pathogens within 1 day, filter-based pathogen enrichment technology was developed utilizing a unique combination of glass fiber depth filter and porous filter aid materials to efficiently separate pathogens from food homogenates and avoid filter clogging by food particles. After pathogen immobilization in depth filters, only viable pathogens were selectively collected in a small volume of growth medium via microbial multiplication and migration; nonviable pathogens remained inside the filters. By assaying viable pathogens using real-time PCRs, multiple species of foodborne pathogens were detected, including L. monocytogenes, Salmonella enterica, and Escherichia coli O157:H7, at around 1 CFU/ml or 1 CFU/g in various food samples. This filter-based pathogen enrichment technology is a unique bacterial enrichment alternative to the conventional culture enrichment step and can significantly shorten the time necessary to obtain assay results.
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BAILEY, J. S., D. L. FLETCHER e N. A. COX. "Effect of Enrichment Media and Sampling Protocol on Recovery of Listeria monocytogenes". Journal of Food Protection 53, n.º 6 (1 de junho de 1990): 505–7. http://dx.doi.org/10.4315/0362-028x-53.6.505.

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These studies examined the differences in recovery of Listeria monocytogenes from pure culture and in the populations of mixed aerobic microflora from chicken and Brie cheese incubated in University of Vermont (UVM) and Listeria enrichment broth (LEB) enrichment broths for different times and conditions. No significant differences were observed in levels of L. monocytogenes from pure cultures in UVM or LEB on any sampling day. No differences were observed in the levels of mixed microflora from Brie cheese in either UVM or LEB, but from chicken rinse the level of mixed flora competitors was significantly higher on all sampling days in LEB as compared to UVM. No differences were observed between a single enrichment in UVM or LEB for 2 d and a transfer to a secondary enrichment tube after 1 d. Overall, the level of mixed microflora capable of growing in enrichment broths was greater from chicken rinse than from Brie cheese. The ratio of L. monocytogenes to mixed microflora which survived the selective enrichments was most favorable for recovery of L. monocytogenes after 2 d of enrichment.
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Teses / dissertações sobre o assunto "Enrichment culture"

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Fernandez, Samuel. "Popular religiosity and Hispanic liturgy toward a mutual enrichment /". Theological Research Exchange Network (TREN), 1992. http://www.tren.com.

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Miller, Stephanie Renee. "Assessment of Nycodenz gradient on enrichment and culture of perinatal porcine spermatogonial stem cells". NCSU, 2006. http://www.lib.ncsu.edu/theses/available/etd-11092006-163407/.

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The objective of this study was to assess the effectiveness of a Nycodenz gradient enrichment method to enrich a dissociated single cell suspension of porcine testicular cells for spermatogonia, and to observe the separated fractions from the gradient over a 14-day culture period for cell viability and number of spermatogonia in culture. Two germ cell specific genes, VASA and DAZL, were utilized for detection of spermatogonia using immunohistochemistry. The control group included cultures generated from the enzymatic digestion of porcine testes prior to the enrichment protocol for each replicate. The NycoDenz gradient consistently separated the isolated cell suspension into three distinct layers and a pellet, all of which were assessed for spermatogonial enrichment. Testis cells were isolated and seeded in culture on day 0. Cell viability and percent of spermatogonia was assessed on day 0, 7, and 14 of culture. Viability was determined using trypan blue exclusion assay and quantified using a hemocytometer. Spermatogonia were morphologically identified as round, plump cells with a large amount of cytoplasm. Visualization of spermatogonia was facilitated by immunostaining with DAZL and VASA polyclonal antibodies and cells exhibiting morphological characteristics in addition to bright, concentrated fluorescence were counted as spermatogonia.
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Grayson, John David. "Improvement of Yellow Perch Larvae Culture via Live Food Enrichment with Polyunsaturated Fatty Acids". The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1417720966.

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Fortress, Ashley M. "A PROFILE OF NEUROGENIC ACTIVITY IN THE AGING HIPPOCAMPAL FORMATION: A CLOSER LOOK AT THE ROLE OF EXERCISE AND ENVIRONMENTAL ENRICHMENT IN THE SAMP-8". Bowling Green, Ohio : Bowling Green State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1178197418.

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Chen, Chao [Verfasser]. "Enrichment of cancer stem cells from head and neck cancer by anchorage independent culture and related research / Chao Chen". Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1068208856/34.

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Wood, Elizabeth. "Development and evaluation of an enrichment culture for bioaugmentation of the P-area chlorinated ethene plume at the Savannah River Site". Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1193079386/.

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Eaddy, Ashley. "Scale-up and characterization of an enrichment culture for bioaugmentation of the P-area chlorinated ethene plume at the Savannah River Site". Connect to this title online, 2008. http://etd.lib.clemson.edu/documents/1219953427/.

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Borisova, Ralitsa Bogomilova. "Isolation of a Rhodococcus Soil Bacterium that Produces a Strong Antibacterial Compound". Digital Commons @ East Tennessee State University, 2011. https://dc.etsu.edu/etd/1388.

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Rhodococci are notable for their ability to degrade a variety of natural and xenobiotic compounds. Recently, interest in Rhodococcus has increased due to the discovery of a large number of genes for secondary metabolism. Only a few secondary metabolites have been characterized from the rhodococci (including 3 recently described antibiotics). Twenty-four new Rhodococcus strains were isolated from soils in East Tennessee using acetonitrile enrichment culturing and identified using 16S rRNA analysis. Forty-seven Rhodococcus strains were screened for antibiotic production using a growth inhibition assay. One strain, MTM3W5.2, had 90% similarity to the Rhodococcus opacus 16S rRNA gene sequence and produced a large zone of inhibition against R. erythropolis and a large number of closely related species. The antimicrobial compound produced by MTM3W5.2 had a large MW of 911.5452 Da and acts much like a bacteriocin but no amino acids were detected in this molecule based on TLC analysis.
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Watson, Claire F. I. "Social contagion in common marmosets (Callithrix jacchus) : implications for cognition, culture and welfare". Thesis, University of Stirling, 2011. http://hdl.handle.net/1893/3446.

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The social transmission of social behaviours in nonhuman primates has been understudied, experimentally, relative to instrumental, food-related behaviours. This is disproportional in relation to the comparatively high percentage of potential social traditions reported in wild primates. I report a systematic survey of the social learning literature and provide quantitative evidence of the discrepancy (Watson and Caldwell, 2009). Addressing the identified deficit in experimental work on social behaviours, I also report three empirical studies investigating the contagious nature of affective states in captive, socially housed marmosets. I carried out an observational study, to determine whether marmosets are influenced by spontaneously produced neighbour calls to perform a range of behaviours associated with similar affect. My results supported a neighbour effect for anxiety in marmosets. Consistent with previous findings for chimpanzees (Baker and Aureli, 1996; Videan et al., 2005), I also found evidence for neighbour effects for aggression and affiliation (Watson and Caldwell, 2010). Through experimental playback, I investigated contingent social contagion in the auditory and visual modalities. The playback of pre-recorded affiliative (chirp) calls was found to be associated with marmosets spending increased time in a range of affiliative behaviours. Playback of video showing conspecifics engaged in a positive affiliative behaviour (allogrooming) also appeared to cause marmosets to spend longer performing various affiliative behaviours. My results indicate that social contagion of affiliation is a multi-modal phenomenon in marmosets and also represent the first evidence that allogrooming is visually contagious in primates. Sapolsky (2006) conceptualised culture as the performance of species-typical behaviours to an unusual extent, termed ‘social culture’. Researchers have yet to directly investigate a transmission mechanism. I investigated whether a social culture of increased affiliation could be initiated in marmosets through the long-term playback, of positive calls, or of video of positive behaviour. The results were consistent with a relatively long-lasting influence of the playback of affiliative calls across several affiliative behaviours. The effect appeared to last substantially beyond the specific hours of playback, between playbacks, and after playback had ceased, potentially indicating a temporary shift in social culture. These results are preliminary but provide some support for the proposal that auditory social contagion may be a transmission mechanism for social culture. The long-term video playback of allogrooming appeared to result in a transitory shift in performance of the identical behaviour (increased allogrooming) after playbacks had ceased. In addition to theoretical implications for social cognition and social culture, my findings have potential practical application for the enhancement of welfare in captive marmosets through sensory, and non-contact social, enrichment.
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Kölschbach, Janina Stephanie [Verfasser], Tillmann [Akademischer Betreuer] [Gutachter] Lüders, Rainer U. [Gutachter] Meckenstock e Johann [Gutachter] Heider. "Identification of naphthalene carboxylase subunits of the sulfate-reducing enrichment culture N47 / Janina Stephanie Kölschbach ; Gutachter: Rainer U. Meckenstock, Johann Heider, Tillmann Lüders ; Betreuer: Tillmann Lüders". München : Universitätsbibliothek der TU München, 2015. http://d-nb.info/1121779883/34.

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Livros sobre o assunto "Enrichment culture"

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Reinhardt, Viktor. Environmental enrichment for caged rhesus macaques: A photographic documentation and literature review. 2a ed. Washington, DC: Animal Welfare Institute, 2001.

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Young, Robert J. Environmental Enrichment for Captive Animals. New York: John Wiley & Sons, Ltd., 2007.

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Environmental enrichment for captive animals. Oxford, UK: Blackwell Science, 2003.

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Adrian, Neal R. RDX biodegradation by a methanogenic enrichment culture obtained from an explosives manufacturing wastewater treatment plant. [Champaign, IL]: US Army Corps of Engineers, Construction Engineering Research Laboratories, 1998.

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ʻAlī, Aḥmad ʻAbd Allāh Aḥmad. al- Taʻallum al-dhātī: Bayna al-naẓarīyah wa-al-taṭbīq. al-Kuwayt: Dhāt al-Salāsil, 1987.

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International Conference on Environmental Enrichment (3rd 1997 Orlando, Fla.). Proceedings of the Third International Conference on Environmental Enrichment, 12 to 17 October 1997, Orlando, Florida. San Diego, CA: Shape of Enrichment, 1998.

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Kach'uk i haengbok haeya in'gan i kŏn'gang hada: Kach'uk sayuk, kongjang kwa nongjang sai ŭi tillema. Sŏul-si: Kaema Kowŏn, 2012.

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Workshop for American Indian Educators on the Learning Potential Assessment Device and Instrumental Enrichment Programs (1982 Shiprock, N.M.). To sing our own songs: Cognition and culture in Indian education : report from a Workshop for American Indian Educators on the Learning Potential Assessment Device and Instrumental Enrichment Programs, Shiprock, Navajo Nation, New Mexico. New York: Association on American Indian Affairs, 1985.

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Wehr, Stephan Dieter. Characterization of anaerobic dechlorinating enrichment cultures maintained on diferent chlorinated ethenes. Ottawa: National Library of Canada, 2001.

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Fink, Stephen L. High commitment workplaces. New York: Quorum Books, 1992.

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Capítulos de livros sobre o assunto "Enrichment culture"

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Wang, Depei, Yingying Wang, Kele Li e Ying Yang. "Research on Enrichment Culture of Bacillus subtilis BI1". In Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), 259–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37916-1_27.

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Bañón-Gomis, Alexis J. "Understanding Entrepreneurship Through the Enrichment of Institutional Theory by Ethics". In Entrepreneurship, Regional Development and Culture, 179–90. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-15111-3_11.

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Mukherjee, Nabanita, Karoline A. Lambert, David A. Norris e Yiqun G. Shellman. "Enrichment of Melanoma Stem-Like Cells via Sphere Assays". In Methods in Molecular Biology, 185–99. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1205-7_14.

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AbstractSphere assays are widely used in vitro techniques to enrich and evaluate the stem-like cell behavior of both normal and cancer cells. Utilizing three-dimensional in vitro sphere culture conditions provide a better representation of tumor growth in vivo than the more common monolayer cultures. We describe how to perform primary and secondary sphere assays, used for the enrichment and self-renewability studies of melanoma/melanocyte stem-like cells. Spheres are generated by growing melanoma cells at low density in nonadherent conditions with stem cell media. We provide protocols for preparing inexpensive and versatile polyHEMA-coated plates, setting up primary and secondary sphere assays in almost any tissue culture format and quantification methods using standard inverted microscopy. Our protocol is easily adaptable to laboratories with basic cell culture capabilities, without the need for expensive fluidic instruments.
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Mouromtsev, Dmitry, Peter Haase, Eugene Cherny, Dmitry Pavlov, Alexey Andreev e Anna Spiridonova. "Towards the Russian Linked Culture Cloud: Data Enrichment and Publishing". In The Semantic Web. Latest Advances and New Domains, 637–51. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-18818-8_39.

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Kloss, M., K. H. Iwannek, I. Fendrik e E. G. Niemann. "Enrichment of diazotrophic bacteria from rice soil in continuous culture". In Nitrogen Fixation with Non-Legumes, 151–64. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4378-0_12.

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Hoch, Alexandra S. H., Kerstin Zörner, Stefanie Kattenbach, Mayke Busch, Erik Schneider, Iris Bürger e Ulf Bethke. "A New System for the Enrichment of Cell Subclones Secreting High Levels of IgG Using Magnetic Cell Sorting (MACS® Technology)". In Cells and Culture, 771–75. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_134.

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Kamiya, Akihide, e Hiromitsu Nakauchi. "Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development". In Methods in Molecular Biology, 273–86. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-125-7_16.

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De Riek, J., e J. Van Huylenbroeck. "Acclimatization of micropropagated roses in multilayer-cells: effect of different stage III conditions and CO2-enrichment". In Physiology, Growth and Development of Plants in Culture, 309–13. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0790-7_35.

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Weiss, Tamara, Sabine Taschner-Mandl, Peter F. Ambros e Inge M. Ambros. "Detailed Protocols for the Isolation, Culture, Enrichment and Immunostaining of Primary Human Schwann Cells". In Methods in Molecular Biology, 67–86. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7649-2_5.

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Conner, Julie A., Robert R. Beitle, Kathleen Duncan, Ravi Kolhatkar e Kerry L. Sublette. "Biotreatment of Refinery Spent-Sulfidic Caustic Using an Enrichment Culture Immobilized in a Novel Support Matrix". In Twenty-First Symposium on Biotechnology for Fuels and Chemicals, 707–19. Totowa, NJ: Humana Press, 2000. http://dx.doi.org/10.1007/978-1-4612-1392-5_54.

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Trabalhos de conferências sobre o assunto "Enrichment culture"

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Kristanto, TMA. "Enrichment of Indonesian Culture through Translation". In 1st International Conference on Interdisciplinary Arts and Humanities. SCITEPRESS - Science and Technology Publications, 2019. http://dx.doi.org/10.5220/0008545100510058.

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Yulianeta, Dewi Prajnaparamitha Amandangi, Halimah e Suci Sundusiah. "BIPA Students’ Responses towards Web-Based Indonesian Folklore Enrichment Materials". In 4th International Conference on Language, Literature, Culture, and Education (ICOLLITE 2020). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.201215.129.

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Nurtdnova, Gulnara, Marina Vinnikova, Guzel Fassahova e Alfiya Yarhamova. "TATAR CULTURE-SPECIFIC CONCEPTS AS A SOURCE OF GLOBAL ENGLISH VOCABULARY ENRICHMENT". In 15th International Technology, Education and Development Conference. IATED, 2021. http://dx.doi.org/10.21125/inted.2021.0802.

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Хинчагашвили, Н. Ш. "СТРАНОВЕДЕНИЕ РОССИИ В ПРАКТИКЕ ОБУЧЕНИЯ ГРУЗИНСКИХ УЧАЩИХСЯ РУССКОМУ ЯЗЫКУ НА НАЧАЛЬНОМ ЭТАПЕ". In Proceedings of the XXVI International Scientific and Practical Conference. RS Global Sp. z O.O., 2021. http://dx.doi.org/10.31435/rsglobal_conf/25022021/7424.

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The article is dedicated to the possibility of the usage of the lingvo-oriented method for the Georgia students in the teaching of Russian as a foreign language. The aim of the article is to promote the enrichment of knowledge in the field of the Russian language through a dialogue of cultural meanings the presented linguo-and methodological culture oriented model of teaching Russian developed and based on communicative and interactive technologies gives the foreign students opportunity to learn about the culture of Russia and to use the acquired knowledge in the process of intercultural communication. The types of interactive exercises discussed in the article can also be used in the study of the history of Russian literature.
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Yuniawan, Tommi, Amin Retnoningsih e Diyamon Prasandha. "Conservation Text for Elementary School Students’ Environmental Literacy Enrichment". In Proceedings of First International Conference on Culture, Education, Linguistics and Literature, CELL 2019, 5-6 August, Purwokerto, Central Java, Indonesia. EAI, 2019. http://dx.doi.org/10.4108/eai.5-8-2019.2289812.

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Kim, Jong-Hoon, Woon-Hong Yeo, Zhiquan Shu, Shinnosuke Inoue, Kieseok Oh, Dayong Gao, Jae-Hyun Chung e Kyong-Hoon Lee. "Tip Enrichment System for Rapid Screening of Mycobacterium Tuberculosis". In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-38403.

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Rapid detection of tuberculosis (TB) has been critically demanded over the last century. To detect TB, numerous methods screening Mycobacterium tuberculosis (MTB) have been developed. However, the methods still have challenges of rapid and specific enrichment of MTB. In this study, we present a novel specific enrichment method of MTB using a microfabricated tip. Through our simulation study, a wavy-shaped microtip is designed to enhance capturing efficiency of bacteria. Using an optimized tip, bacteria are attracted by dielectrophoresis and captured by affinity binding and capillary action. In experiment, a surrogate marker of MTB, Bacilli Calmette-Guerin (BCG), is enriched with the micromachined tip. When a microtip decorated with capturing antibodies is used, BCG cells spiked in saliva are detected at the concentration of 5×105 CFU/mL within 20 minutes. The tip enrichment system demonstrates the potential for rapid, culture-free detection of MTB in a raw sample.
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Mumrikova, Larisa Ivanovna. "Theoretical Aspects of Ecological Culture Development in the Context of Primary School Students' Self-Enrichment". In International Scientific and Practical Conference. TSNS Interaktiv Plus, 2020. http://dx.doi.org/10.21661/r-551620.

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Rabionet, Marc, Sonia Palomeras, Ines Ferrer, Ariadna Sarrats, Ariadna Giro-Perafita, Maria Luisa Garcia-Romeu, Joaquim Ciurana e Teresa Puig. "Abstract 3323: Breast cancer stem cell culture and enrichment using poly(ε-caprolactone) 3D scaffolds". In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-3323.

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Simon, Karen A., Sylaja Murikipudi, Harry A. Rogoff e Chiang J. Li. "Abstract 2532: Alginate-based 3D system for the enrichment and culture of cancer stem cells". In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-2532.

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Armiati, Dessi Susanti e Rose Rahmidani. "The Validity Analysis of Economic Enrichment Book Based on Minangkabau Culture for Senior High Schools". In 2nd Progress in Social Science, Humanities and Education Research Symposium (PSSHERS 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210618.061.

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Relatórios de organizações sobre o assunto "Enrichment culture"

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Boopathy, R., e J. Manning. Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture. Office of Scientific and Technical Information (OSTI), agosto de 1997. http://dx.doi.org/10.2172/515534.

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Phelps, T. J., D. Ringelberg, A. T. Mikell, D. C. White e C. B. Fliermans. Mineralization of trichloroethylene by heterotrophic enrichment cultures. Office of Scientific and Technical Information (OSTI), dezembro de 1988. http://dx.doi.org/10.2172/666263.

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