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Artigos de revistas sobre o assunto "Flax oil"

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Alp, Hayriye. "Flax Seed". Gastroenterology Pancreatology and Hepatobilary Disorders 5, n.º 5 (10 de setembro de 2021): 01–02. http://dx.doi.org/10.31579/2641-5194/045.

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Flax sed also known as flax oil and linseed oil, is derived from the seeds of the plant Linium usitatissimum. Flax seed oil is a very rich source of alpha-linolenic acid. Alpha-linolenic acid concentration in flaxseed oil ranges from approximately 40 to 60%.lower amounts of linoleic acid and oleic acid (each about 15%) are also present in flaxseed oil.ın addition, flaxseed contains varying amounts of the lignan, secoisolariciresinol diglycoside (SDG).
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Bauerová, Pavla, Pavel Reiterman, Milena Pavlíková, Magdalena Kracík Štorkánová e Martin Keppert. "FRESH STATE PROPERTIES OF LIME MORTARS WITH FLAX OIL ADMIXTURE". Acta Polytechnica CTU Proceedings 22 (25 de julho de 2019): 7–11. http://dx.doi.org/10.14311/app.2019.22.0007.

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Flax oil has been used as mortar improving, hydrophobic, admixture already in ancient times. The paper describes the identification of flax oil in mosaic mortar from ca. 1900 by help of FTIR spectroscopy. This historic mortar was reproduced by nowadays raw materials and the influence of flax oil on consistency and air entraining of fresh mortar was tested. It was found that already small amount of oil caused significant air entrainment, which, simultaneously with water repellency action, may contribute to higher durability of mortar with oil admixture. The flux oil influenced also consistency of mortar; its presence caused higher flow value of mortar. Moreover, the introduction of the paper summarizes principal knowledge about action of natural admixtures in lime mortars and plasters on basis of current literature.
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ROWLAND, G. G., R. S. BHATTY e E. O. KENASCHUK. "SOMME FLAX". Canadian Journal of Plant Science 70, n.º 2 (1 de abril de 1990): 545–46. http://dx.doi.org/10.4141/cjps90-066.

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Somme, a medium early-maturing oilseed flax (Linum usitatissimum) with increased yield potential over other medium early cultivars, has been released by the Crop Development Centre, University of Saskatchewan. It is of medium seed size, average oil and protein content and higher oil quality. Somme is resistant to all North American races of rust caused by Melampsora lini and is moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini.Key words: Linum usitatissimum, flax (oilseed), cultivar description
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Duguid, S. D., E. O. Kenaschuk e K. Y. Rashid. "Hanley Flax". Canadian Journal of Plant Science 83, n.º 1 (1 de janeiro de 2003): 85–87. http://dx.doi.org/10.4141/p02-050.

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Hanley, a medium maturing oilseed flax (Linum usitatissimum L.) was released in 2001 by Agriculture and Agri-Food Canada, Morden Research Station, Morden, Manitoba. This cultivar has medium oil content, very high oil quality, medium seed size, very good lodging resistance, and high yield when seeded in all soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini (Ehrenb.) Desmaz, and resistant to wilt caused by Fusarium oxysporum f. sp. lini Schlecht. f. sp. lini (Bolley) Snyder & Hansen. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Duguid, S. D., E. O. Kenaschuk e K. Y. Rashid. "Lightning flax". Canadian Journal of Plant Science 83, n.º 1 (1 de janeiro de 2003): 89–91. http://dx.doi.org/10.4141/p02-051.

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Lightning, a medium-late maturing oilseed flax (Linum usitatissimum L.) was released in 2001 by Agriculture and Agri-Food Canada, Morden Research Station, Morden, Manitoba. This cultivar has high oil content, high oil quality, large seed size, good lodging resistance and high yield when seeded in the Black, Brown and Dark Brown soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini (Ehrenb.) Desmaz, and resistant to wilt caused by Fusarium oxysporum Schlecht. f. sp. lini (Bolley) Snyder & Hansen. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Duguid, S. D., E. O. Kenaschuk e K. Y. Rashid. "Macbeth flax". Canadian Journal of Plant Science 83, n.º 4 (1 de outubro de 2003): 803–5. http://dx.doi.org/10.4141/p03-026.

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Macbeth, a medium-late maturing oilseed flax (Linum usitatissimum L.) was released in 2002 by Agriculture and Agri-Food Canada, Morden Research Station, Morden, Manitoba. This cultivar has high oil content, high oil quality, large seed size, very good lodging resistance and high yield when seeded in all the soil zones of the Canadian prairies as compared to Flanders. It is immune to current North American races of rust caused by Melampsora lini (Ehrenb.) Desmaz,, moderate resistance to wilt by Fusarium oxysporum Schlecht. f. sp. lini (Bolley) Snyder & Hansen and moderate resistance to powdery mildew by Oidium lini Skoric. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Duguid, S. D., K. Y. Rashid e E. O. Kenaschuk. "Shape flax". Canadian Journal of Plant Science 94, n.º 1 (janeiro de 2014): 157–60. http://dx.doi.org/10.4141/cjps2013-197.

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Duguid, S. D., Rashid, K. Y. and Kenaschuk, E. O. 2014. Shape flax. Can. J. Plant Sci. 94: 157–160. Shape, a medium maturing oilseed flax (Linum usitatissimum L.), was released in 2007 by Agriculture and Agri-Food Canada, Morden Research Station, Morden, Manitoba. Developed from the cross M4684/FP1043 made in 1997, Shape was evaluated in the Flax Cooperative Trials in (2004–2006) before being registered in 2007. Shape's desirable combination of agronomic traits and seed quality, particularly its significantly improved oil and protein content, oil quality profile and disease resistance, should make it a useful and unique cultivar for producers and the flax industry.
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Côrtes, Cristiano, Ricardo Kazama, Daniele da Silva-Kazama, Chaouki Benchaar, Lucia M. Zeoula, Geraldo TD Santos e Hélène V. Petit. "Digestion, milk production and milk fatty acid profile of dairy cows fed flax hulls and infused with flax oil in the abomasum". Journal of Dairy Research 78, n.º 3 (21 de julho de 2011): 293–300. http://dx.doi.org/10.1017/s0022029911000446.

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Flax hull, a co-product obtained from flax processing, is a rich source of n-3 fatty acids (FA) but there is little information on digestion of flax hull based diets and nutritive value of flax hull for dairy production. Flax oil is rich in α-linolenic acid (LNA) and rumen bypass of flax oil contributes to increase n-3 FA proportions in milk. Therefore, the main objective of the experiment was to determine the effects of abomasal infusion of increasing amounts of flax oil on apparent digestibility, dry matter (DM) intake, milk production, milk composition, and milk FA profile with emphasis on the proportion of LNA when cows were supplemented or not with another source of LNA such as flax hull. Six multiparous Holstein cows averaging 650±36 kg body weight and 95±20 d in milk were assigned to a 6×6 Latin square design (21-d experimental periods) with a 2×3 factorial arrangement of treatments. Treatments were: 1) control, neither flax hull nor flax oil (CON), 2) diet containing (DM basis) 15·9% flaxseed hull (FHU); 3) CON with abomasal infusion of 250 g/d flax oil; 4) CON with abomasal infusion of 500 g/d flax oil; 5) FHU with abomasal infusion of 250 g/d flax oil; 6) FHU with abomasal infusion of 500 g/d flax oil. Infusion of flax oil in the abomasum resulted in a more pronounce decrease in DM intake for cows fed the CON diets than for those fed the FHU diets. Abomasal infusion of flax oil had little effect on digestibility and FHU supplementation increased digestibility of DM and crude protein. Milk yield was not changed by abomasal infusion of flax oil where it was decreased with FHU supplementation. Cows fed FHU had higher proportions of 18:0, cis9-18:1, trans dienes, trans monoenes and total trans in milk fat than those fed CON. Proportion of LNA was similar in milk fat of cows infused with 250 and 500 g/d flax oil in the abomasum. Independently of the basal diet, abomasal infusion of flax oil resulted in the lowest n-6:n-3 FA ratio in milk fat, suggesting that the most important factor for modification of milk FA profile was the amount of n-3 FA bypassing the rumen and not the amount of flax hull fed to dairy cows. Moreover, these data suggest that there is no advantage to supply more than 250 g/d of flax oil in the abomasum to increase the proportion of LNA in milk fat.
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Ryabenko, L. G., V. S. Zelentsov, L. R. Ovcharova, G. G. Galkina, S. V. Sklyarov, S. V. Zelentsov e E. V. Moshnenko. "The oil flax variety RFN". Oil Crops 177, n.º 1 (25 de maio de 2019): 143–45. http://dx.doi.org/10.25230/2412-608x-2019-1-177-143-145.

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Sukeymenova, A. K., e I. A. Loshkomoynikov. "The oil flax variety Amber". Oil Crops 184, n.º 4 (25 de dezembro de 2020): 103–5. http://dx.doi.org/10.25230/2412-608x-2020-4-184-103-105.

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To use flax oil in food, it is necessary to decrease the content of linolenic acid. We developed the oil flax variety Amber by individual selection from the hybrid population of the 3rd generation from crossing of the low-linolenic variety Linol and the breeding line 34725. The variety is mid-ripening, the duration of its growth season is 90-98 days, it is well adapted to the soil and climatic conditions of Siberia. It differs from the standard variety by its low content of linolenic acid in oil – 4.8-6.3 %. According to the results of competitive variety testing in 2016-2018, the variety Amber exceeded the standard variety Severny in terms of seed productivity by 0.29 t/ha. The variety ripens in uniformity, it is resistant to Fusarium blight, lodging and shedding, it is suitable for mechanized harvesting, it is developed to obtain edible oil of high quality. The potential cultivation areas of the oil flax variety are Volgo-Vyatsky region, Ural and West Siberia.
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Teses / dissertações sobre o assunto "Flax oil"

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Casey, John. "EFFECTS OF STEARIDONIC ACID-ENRICHED SOYBEAN OIL ON METABOLIC PROFILE AND FATTY ACID COMPOSITION IN LEAN AND OBESE ZUCKER RATS". OpenSIUC, 2013. https://opensiuc.lib.siu.edu/theses/1312.

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Background, Consumption of marine-based oils high in omega-3 polyunsaturated fatty acids (n3PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is known to protect against obesity-related pathologies. It is less clear whether traditional vegetable oils with high omega-6 polyunsaturated fatty acid (n6PUFA) content exhibit similar therapeutic benefits. As such, this study examined the metabolic effects of a plant-based n3PUFA, stearidonic acid (SDA), in polygenic obese rodents. Methods, Lean (LZR) and obese Zucker (OZR) rats were provided either a standard westernized control diet (CON) with a high n6PUFA to n3PUFA ratio (i.e., 16.2/1.0) or experimental diet modified with flaxseed (FLAX), menhaden (FISH), or SDA oil that resulted in n6PUFA to n3PUFA ratios of 1.7/1.0, 1.3/1.0, and 1.0/0.8, respectively. Results, After 12 weeks, total adiposity, dyslipidemia, glucose intolerance, and hepatic steatosis were all greater, whereas n3PUFA content in liver, adipose, and muscle was lower in OZR vs. LZR rats. Obese rodents fed modified FISH or SDA diets had lower serum lipids and hepatic fat content vs. CON. The omega-3 index (i.e., ÓEPA + DHA in erythrocyte membrane) was 4.0, 2.4, and 2.0-fold greater in rodents provided FISH, SDA, and FLAX vs. CON diet, irrespective of genotype. Total hepatic n3PUFA and DHA was highest in rats fed FISH, whereas both hepatic and extra-hepatic EPA was higher with FISH and SDA groups. Conclusions, these data indicate that SDA oil represents a viable plant-derived source of n3PUFA, which has therapeutic implications for several obesity-related pathologies.
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Walton, John-Peter. "The effect of dietary flax oil and antioxidants on pulmonary hypertension and ascites in broiler chickens". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ40448.pdf.

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Jamboonsri, Watchareewan. "IMPROVEMENT OF NEW OIL CROPS FOR KENTUCKY". UKnowledge, 2010. http://uknowledge.uky.edu/gradschool_diss/120.

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Three oil crops, chia (Salvia hispanica L.), flax (Linum usitatissimum L.), and castor (Ricinus communis L.), were studied because of their nutritional and industrial values. Chia and flax are rich in an ω3 fatty acid, α-linolenic acid, and castor is a very high oil producer and high in a hydroxy fatty acid. Ethyl methanesulfonate (EMS) and gamma rays were employed to mutagenize chia seeds to produce early flowering mutants. The M1 population was grown and induced to flower by short-day photoperiods. The M2 population was planted in the field in Lexington, KY in 2008. Early flowering plants were found 55 days after planting while non-mutagenized plants did not produce any flower buds until the 7th of October, 82 days after planting, at a daylength of 11 hours and 32 minutes. 0.012% of the EMS-treated M2 population and 0.024% of the gamma radiation-treated population flowered much earlier than the controls. M3 early flowering mutant lines were able to flower at photoperiods of 12-15 hours in a greenhouse. Selected lines produced flower buds on the 7th of July, 47 days after planting, at a daylength of 14 hours and 41 minutes in the field in Lexington, Kentucky. Different varieties of flax were evaluated for seed yield and field performance in Kentucky. Plant height and yield data were collected from three growing seasons. Yields from 2006 trial varied from 368-1,267 kg/ha. Yields from 2007 and 2008 were much lower due to drought. The variety ‘Carter’ gave the highest yield every season. Flax can be grown in Kentucky but yields are low. Two high-yield castor varieties, ‘Carmencita’ and ‘TTU-LRC’, were crossed in greenhouse. The F1 population was grown in the field. Inflorescences were covered to ensure self-pollination. The F2 population showed a high degree of segregation for plant height, stem color, capsule color and seed yield in the following growing season. Data on plant height, number of branches, color, and yield was collected from 89 F2 individuals. Fifteen lines with the highest yield were selected to plant in the field in spring of 2009. New high-yield castor varieties are being developed for production in Kentucky.
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Arnott, Karen Lesley. "Evaluation of garlic powder supplementation with and without flax oil supplementation on blood lipid levels in women". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0021/MQ55649.pdf.

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Pu, Shuaihua. "The Effect of Consuming Canola and Flax Oils in Modulation of Vascular Function and Biomarkers of Cardiovascular Disease RisksThe Effect of Consuming Canola and Flax Oils in Modulation of Vascular Function and Biomarkers of Cardiovascular Disease Risks". BioMed Central, the American Society for Nutrition, Cambridge, 2016. http://hdl.handle.net/1993/31256.

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It is well established that replacing dietary saturated fatty acids with unsaturated fatty acids reduces cardiovascular disease (CVD) risk. Although epidemiological and clinical evidence indicate health benefits of consuming various fatty acid classes including n-9, n-6, and short- and longer-chain n-3 fatty acids, current dietary recommendations fall short of providing the optimal amounts of these fatty acids in daily diets. In addition, significant knowledge gaps remain in our understanding of the effects of, and mechanisms underpinning the action of, the various fatty acid classes on risk factors for CVD. The objective of this research was to contribute to the evaluation of health benefits of using different dietary oils, and determine how these benefits may play a role in improving public health and decreasing CVD risk. Additionally, this research examined effects of diet-gene interactions, endogenous fatty acid ethanolamides (FAEs) on body fat mass distribution as well as changes in the composition of gut microbiota following consumption of dietary oil treatments. The Canola Oil Multicenter Intervention Trial (COMIT) was conducted as a 5-phase randomized, controlled, double-blind, cross-over clinical trial. Each 4-wk treatment period was separated by 4-wk washout intervals. A total of 130 volunteers with abdominal obesity consumed each of 5 identical weight-maintaining, fixed-composition diets with one of the following treatment oils (60 g/3000 kcal) in the form of beverages: 1) conventional canola oil (Canola; n-9 rich), 2) high–oleic acid canola oil with docosahexaenoic acid (CanolaDHA; n-9 and n-3 rich), 3) a blend of corn and safflower oil (25:75) (CornSaff; n-6 rich), 4) a blend of flax and safflower oils (60:40) (FlaxSaff; n-6 and short-chain n-3 rich), and 5) high–oleic acid canola oil (CanolaOleic; highest in n-9). At endpoints, plasma fatty acid levels reflected the differences in fatty acid composition of five dietary treatments. All diets lowered total cholesterol (TC) compared with baseline. TC was lowest after the FlaxSaff phase and highest after CanolaDHA. The CanolaDHA treatment improved HDL-C, triglycerides, and blood pressure thereby reducing Framingham risk scores compared with other oils varying in unsaturated fatty acid composition. Homozygotes minor allele carriers of rs174583 (TT) on FADS2 gene showed lower (P<0.01) plasma EPA and DPA levels across all diets, but no differences were observed in DHA concentrations after the CanolaDHA feeding. In addition, plasma FAE levels were positively associated with plasma fatty acid profiles. Minor allele A carriers of rs324420 of FAAH gene showed a higher (P<0.05) plasma FAE levels compared with major allele C carriers across all diets, and showed higher (P=0.0002) docosahexaenoylethanolamide levels in response to the CanolaDHA diet. Impacts of consuming 60 g of five dietary oil treatments on gut microbiota composition were relatively minor at the phylum level and mainly at the genus level, while BMI contributed to a significant shift at the phylum level. In conclusion, consumption of a novel DHA-enriched canola oil improved blood lipid profile and largely reduced CVD risk. Diet-gene interactions might help identify sub-populations who appear to benefit from increased consumption of DHA and oleic acid. The metabolic and physiological responses to dietary fatty acids may be influenced via circulating FAEs, while the altered microbiota profile by shifts in MUFA and/or PUFA may be associated with specific physiological effect. Personalized diets varying in unsaturated fatty acids composition based on specific lifestyles, environmental factors, psychosocial factors, and genetic make-ups will become the future “healthy eating” recommendations to prevent CVD risk.
May 2016
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Cardoso, Cristóvão dos Santos. "Utilização de alimentos alternativos na dieta do coelho : gordura de insetos vs gordura de linho". Master's thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, Instituto Superior de Agronomia, 2018. http://hdl.handle.net/10400.5/15158.

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Dissertação de Mestrado em Engenharia Zootécnica/Produção Animal
Atualmente, a nível mundial, configura-se a perspetiva de um aumento de consumo de insetos tanto pelos humanos como pelos animais, pelo que se considera importante a realização de diferentes estudos, nomeadamente da sua utilização nas dietas animais. Com o objetivo de comparar o efeito do uso de duas gorduras suplementadas na dieta de coelhos, nomeadamente de origem animal e vegetal (óleo de Hermetia illucens, e de linho, respetivamente), foram formulados 4 regimes alimentares com teor baixo (5%) e com teor alto (8%) de gordura, para cada fonte de gordura adicionada, e distribuídos a 48 coelhos (12 x 4) com 35 dias (recém desmamados) alojados em gaiolas de digestibilidade. Os animais tiveram acesso ad libitum a alimento e água durante as 5 semanas do perído experimental, sendo abatidos aos 70 dias de idade. Foram avaliados os resultados zootécnicos, sem diferenças significativas (P>0,05) e recolhidas e analisadas as fezes na 4ª semana do ensaio, para se avaliar a digestibilidade aparente das dietas. Os pesos iniciais (1047g) foram uniformes e os finais (2405g) foram semelhantes em todos os grupos. Verificou-se que há um efeito muito significativo na natureza da gordura (P<0,0001), na digestibilidade da matéria seca e na da matéria orgânica. Os regimes com óleo de insetos têm uma digestibilidade da MS e da MO cerca de 95% da digestibilidade das mesmas frações dos regimes com óleo de linho. Também a digestibilidade das frações EE e EB foi significativamente mais baixa (P<0,05) nos regimes com óleo de inseto. Os regimes com teores de gordura mais altos aumentaram a digestibilidade (P<0,0001) do extracto etéreo, mas diminuíram (P<0,003) a digestibilidade da celulose. Foram analisadas as carcaças, e não houveram diferenças significativas (P>0,05) nos pesos do aparelho digestivo, fígado, gorduras peri-renal e inter-escapular, apenas um efeito no peso do estômago vazio (P=0,006), maior nos regimes com óleo de insetos, provavelmente devido a uma maior retenção de alimento durante o período experimental. Entre os resultados colorimétricos obtidos, não parecem haver diferenças significativas, excepto uma tonalidade mais vermelha na carne dos animais dos regimes com óleo de insetos (P=0,047). De acordo com estes resultados a gordura de insetos pode ser utilizada na alimentação do coelho, apenas com alguma perda na digestibilidade da matéria seca e orgânica, mas não afetando o crescimento nem o rendimento da carcaça.
ABSTRACT - Use of alternative foods in the rabbit diet: insect oil vs flax oil - Currently, the world-wide perspective is an increase in the consumption of insects by both humans and animals. Therefore, it is considered important to carry out different studies including their use in animal diets. In order to compare the effect of two fat supplements on the rabbit diet, from animal and vegetable origin (Hermetia illucens oil, and flax/lindseed oil, respectively), four dietary regimens with low (5%) and high (8%) fat content, for each fat souce added, and distributed to 48 rabbits (12 x 4) with 35 days of age (recently weaned) housed in digestibility cages. The animals had access ad libitum to food and water during the 5 weeks of the experimental period, being slaughtered at 70 days of age. The zootechnical results were evaluated, without significant differences (P> 0.05) and feces were collected in the 4th week oh the trial, and analyzed to evaluate the apparent digestibility of the diets. The initial weights (1047g) were uniform and the final weights (2405g) were similar in all groups. It was verified that there is a very significant effect on the nature of fat (P <0.0001), on dry matter and organic matter digestibility. Insect oil regimes have a digestibility of DM and OM about 95% of the digestibility of the same fractions from flax oil regimes. Also, the digestibility of the EE (ethereal extract) and GE (gross energy) fractions were significantly lower (P <0.05) in the insect oil regimens. Regimens with higher fat contents increased the digestibility (P <0.0001) of ethereal extract, but decreased (P <0.003) the cellulose digestibility. Carcasses were analyzed, and there were no significant differences (P> 0.05) in digestive tract, liver, peri-renal and inter-scapular fat weights, only an effect on empty stomach weight (P = 0.006), higher in insect oil regimes, probably due to higher feed retention during the experimental period. Among the colorimetric results obtained, there appear to be no significant differences, except for a reddish in meat of the animals who ate insect oil regimes (P = 0.047). According to these results, insect fat can be used on rabbit diet, with only some slightly lower digestibility in dry matter and organic matter, but not affecting the growth or carcass yield.
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Kadivar, Mahdi. "Studies on integrated processes for the recovery of mucilage, hull, oil and protein from solin (low linolenic acid flax)". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ63883.pdf.

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Almeida, Ana Paula da Silva. "Efeito do óleo de linhaça no desempenho, características de carcaça e qualidade de carne de frangos de corte /". Araçatuba : [s.n.], 2007. http://hdl.handle.net/11449/94753.

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Orientador: Marcos Franke Pinto
Banca: Manoel Garcia Neto
Banca: Massami Shimokomaki
Resumo: O objetivo deste trabalho foi avaliar a incorporação de ácidos graxos poliinsaturados (PUFA) na carne de frango com a utilização de ração enriquecida com óleo de soja e óleo de linhaça, associados a diferentes níveis de vitamina E. A inclusão de óleo de linhaça, substituindo o óleo de soja em diferentes proporções, e de vitamina E até o nível de 400 mg/kg de ração, não interferiu, de forma geral, nas características de desempenho e de composição de carcaça de frangos de corte da linhagem Cobb. O sexo das aves, por outro lado, influenciou significativamente essas características. Os machos apresentaram, em termos gerais, melhor desempenho produtivo que as fêmeas, com maior consumo de ração e ganho de peso e melhor conversão alimentar. As fêmeas apresentaram maior porcentagem de peito e menor porcentagem de coxa e sobrecoxa que os machos. Com relação às características da carne, o sexo e a fonte de ácidos graxos poliinsaturados e os níveis de vitamina E da ração não apresentaram influência no teor de lipídeos totais, na textura e na perda de peso no cozimento. A adição de linhaça na dieta também não modificou o padrão de ácidos graxos, nem o teor de colesterol da carne. Os machos apresentaram maior proporção de PUFA que as fêmeas nos dois cortes analisados. Com relação ao teor de colesterol total, as amostras de peito provenientes dos machos apresentaram médias mais elevadas em relação às das fêmeas. A inclusão de 200mg/kg de vitamina E na ração foram suficientes para impedir a rancidez oxidativa nas amostras de coxa e sobrecoxa com pele, estocadas a -25ºC por 45 dias.
Abstract: The aim of this work was to evaluate the incorporation of polyunsaturated fatty acids (PUFA) in broilers meat using soy and linseed oil enriched rations and different levels of vitamin E. The inclusion of different percentages of linseed oil instead of soy oil and vitamin E until 400 mg/kg in the diets did not interfere in overall performance or composition characteristics of Cobb broilers carcasses. On the other hand, broilers' sexes influenced these characteristics. Male broilers consumed more ration, had higher weight gains and better feed conversions than female broilers. Female broilers had higher breast percentages and lower leg percentages than male broilers. Sex, source of polyunsaturated fatty acids and vitamin E level did not influence total fat content of meat, texture and loose of weight after cooking. The inclusion of linseed oil in the diet did not cause any modification on meat fatty acids composition or total cholesterol content. Male broilers breast and leg samples had higher PUFA content than female broilers. Male broilers had higher cholesterol contents than female broilers in breast cuts. Vitamin E at 200 mg/kg was enough to prevent oxidative rancidity in leg samples frozen at -25ºC for 45 days.
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Almeida, Ana Paula da Silva [UNESP]. "Efeito do óleo de linhaça no desempenho, características de carcaça e qualidade de carne de frangos de corte". Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/94753.

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Made available in DSpace on 2014-06-11T19:27:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-08-20Bitstream added on 2014-06-13T18:55:55Z : No. of bitstreams: 1 almeida_aps_me_araca.pdf: 933088 bytes, checksum: 66f57629124cdebcfa4b653eca08077f (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O objetivo deste trabalho foi avaliar a incorporação de ácidos graxos poliinsaturados (PUFA) na carne de frango com a utilização de ração enriquecida com óleo de soja e óleo de linhaça, associados a diferentes níveis de vitamina E. A inclusão de óleo de linhaça, substituindo o óleo de soja em diferentes proporções, e de vitamina E até o nível de 400 mg/kg de ração, não interferiu, de forma geral, nas características de desempenho e de composição de carcaça de frangos de corte da linhagem Cobb. O sexo das aves, por outro lado, influenciou significativamente essas características. Os machos apresentaram, em termos gerais, melhor desempenho produtivo que as fêmeas, com maior consumo de ração e ganho de peso e melhor conversão alimentar. As fêmeas apresentaram maior porcentagem de peito e menor porcentagem de coxa e sobrecoxa que os machos. Com relação às características da carne, o sexo e a fonte de ácidos graxos poliinsaturados e os níveis de vitamina E da ração não apresentaram influência no teor de lipídeos totais, na textura e na perda de peso no cozimento. A adição de linhaça na dieta também não modificou o padrão de ácidos graxos, nem o teor de colesterol da carne. Os machos apresentaram maior proporção de PUFA que as fêmeas nos dois cortes analisados. Com relação ao teor de colesterol total, as amostras de peito provenientes dos machos apresentaram médias mais elevadas em relação às das fêmeas. A inclusão de 200mg/kg de vitamina E na ração foram suficientes para impedir a rancidez oxidativa nas amostras de coxa e sobrecoxa com pele, estocadas a -25ºC por 45 dias.
The aim of this work was to evaluate the incorporation of polyunsaturated fatty acids (PUFA) in broilers meat using soy and linseed oil enriched rations and different levels of vitamin E. The inclusion of different percentages of linseed oil instead of soy oil and vitamin E until 400 mg/kg in the diets did not interfere in overall performance or composition characteristics of Cobb broilers carcasses. On the other hand, broilers` sexes influenced these characteristics. Male broilers consumed more ration, had higher weight gains and better feed conversions than female broilers. Female broilers had higher breast percentages and lower leg percentages than male broilers. Sex, source of polyunsaturated fatty acids and vitamin E level did not influence total fat content of meat, texture and loose of weight after cooking. The inclusion of linseed oil in the diet did not cause any modification on meat fatty acids composition or total cholesterol content. Male broilers breast and leg samples had higher PUFA content than female broilers. Male broilers had higher cholesterol contents than female broilers in breast cuts. Vitamin E at 200 mg/kg was enough to prevent oxidative rancidity in leg samples frozen at -25ºC for 45 days.
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Heidor, Renato. "Potencial quimiopreventivo de lipídios estruturados obtidos por interesterificação da tributirina com o óleo de linhaça na hepatocarcinogênese". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/9/9132/tde-30032017-165502/.

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O carcinoma hepatocelular (HCC) apresenta mau prognóstico o que torna importante sua quimioprevenção. Nesse sentido, a tributirina (TB), um inibidor de desacetilases de histonas (HDACi), mostrou-se um quimiopreventivo promissor da hepatocarcinogênese. Avaliaram-se aqui efeitos quimiopreventivos de lipídios estruturados (EST) obtidos por interesterificação enzimática a partir da TB com o óleo de linhaça (LIN). Ratos foram tratados com EST (grupo EST; 165 mg/100g peso corpóreo [p.c]), TB (grupo TB; 200 mg/100g p.c), LIN (grupo LIN; 133 mg/100g p.c), mistura de TB com LIN (grupo LIN; 165 mg/100g p.c) ou maltodextrina (MD) (grupo MD; controle isocalórico; 300 mg/100g p.c) diariamente durante 8 semanas consecutivas por gavagem. Duas semanas após início dos tratamentos, os animais foram submetidos ao modelo de hepatocarcinogênese do \"hepatócito resistente\" (RH). Os grupos EST e TB apresentaram atividade quimiopreventiva bloqueadora e supressora, respectivamente, da hepatocarcinogênese. TB induziu a apoptose, ao contrário dos EST. O tratamento com TB resultou na acetilação e trimetilação da H3K9 e H3K27, enquanto EST atuaram somente na trimetilação das mesmas. Quando analisada a expressão de genes envolvidos com modificações em histonas, EST e TB reduziram a expressão de Ezh2 e de Hdac4. Por outro lado, somente os EST aumentaram a expressão de Hdac6. Tal efeito por parte dos EST merece ser mais investigado, uma vez que esta desacetilase vem sendo sugerida como alvo potencial para o desenvolvimento de fármacos. Em conclusão, a atividade quimiopreventiva de EST e da TB envolve na hepatocarcinogênese experimental mecanismos epigenéticos que podem ou não ser distintos.
Hepatocellular carcinoma (HCC) has a poor prognosis, which makes its chemoprevention important. Tributyrin (TB), which is a histone deacetylase inhibitor (HDACi), is a promising chemopreventive agent of hepatocarcinogenesis. The chemopreventive effects of structured lipids (STLs) that were obtained by the enzymatic interesterification of TB with flaxseed oil (FSO) were evaluated in the present study. Rats were treated with STLs (STL group, 165 mg/100 g body weight (bw)), TB (TB group, 200 mg/100 g bw), FSO (FSO group, 133 mg/100 g bw), TB mixed with FSO (BLD group, 165 mg/100g bw) or maltodextrin (MD) (MD group; isocaloric control; 300 mg/100 g bw) daily for eight consecutive weeks by gavage. Two weeks after the initiation of treatment, the animals were subjected to the resistant hepatocyte hepatocarcinogenesis model (RH). The STL and TB groups developed blocker and suppressive chemopreventive activity against hepatocarcinogenesis, respectively. TB treatment induced apoptosis, unlike the STL treatment. Additionally, TB treatment resulted in the acetylation and trimethylation of H3K9 and H3K27, whereas the STLs acted only in the trimethylation of these histones. When analyzing the expression of genes involved in histone modifications, the STLs and TB reduced enhancer of zeste homolog 2 (Ezh2) and histone deacetylase 4 (Hdac4) gene expression. Conversely, only the STLs increased Hdac6 gene expression. This effect of the STLs warrants further investigation because this deacetylase has been suggested as a potential drug development target. In conclusion, the chemopreventive activities of the STLs and TB in experimental hepatocarcinogenesis involve epigenetic mechanisms that may be distinct.
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Livros sobre o assunto "Flax oil"

1

Beutler, Jade. Flax for life!: 101 delicious recipes and tips featuring fabulous flax oil. Encinitas, CA: Progressive Health Pub., 1996.

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Sylvie, Cloutier, e Ragupathy Raja, eds. Flax lipids: Classes, biosynthesis, genetics and the promise of applied genomics for understanding and altering of fatty acids. Hauppauge, N.Y: Nova Science Publishers, 2010.

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Huiqi, Chen, ed. Tou nao hao de ren dou he ya ma ren you: Chan sheng jue jia xiao guo de yin shi shu = Flax seed oil. Taizhong Shi: Chen xing chu ban you xian gong si, 2012.

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Canada. Parliament. House of Commons. Bill: An act to amend the General inspection act so as to provide a grade for flax seed. Ottawa: S.E. Dawson, 2003.

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5

Lord, Aeck & Sargent Architecture. Dry Tortugas National Park : Dry Tortugas Light Station: Lighthouse and oil house historic structure report. Atlanta, Ga: Cultural Resources, Southeast Region, National Park Service, 2010.

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6

Akcasayar, Nezih. Nucleate pool boiling performance of finned and high flux tube bundles in R-114/oil mixtures. Monterey, Calif: Naval Postgraduate School, 1989.

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7

Beutler, Jade. Flax for Life: 101 Delicious Recipes & Tips Featuring Fabulous Flax Oil. Apple Tree Pub Co Ltd, 1999.

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Beutler, Jade. Flax for Life!: 101 Delicious Recipes and Tips Featuring Fabulous Flax Oil. Apple Publishing Company (WA), 1997.

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MacFadyen, Joshua. Flax Americana: A History of the Fibre and Oil that Covered a Continent. McGill-Queen's University Press, 2018.

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MacFadyen, Joshua. Flax Americana: A History of the Fibre and Oil That Covered a Continent. McGill-Queen's University Press, 2018.

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Capítulos de livros sobre o assunto "Flax oil"

1

Duguid, Scott D. "Flax". In Oil Crops, 233–55. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-77594-4_7.

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Krist, Sabine. "False Flax Oil". In Vegetable Fats and Oils, 317–24. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-30314-3_49.

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Markulin, Lucija, Yuliia Makhno, Samantha Drouet, Sara Zare, Sumaira Anjum, Duangjai Tungmunnithum, Mohammad R. Sabzalian et al. "On “The Most Useful” Oleaginous Seeds: Linum usitatissimum L., A Genomic View with Emphasis on Important Flax Seed Storage Compounds". In Oil Crop Genomics, 135–57. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-70420-9_8.

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Cullis, Christopher A., e Margaret A. Cullis. "Comparison Between the Genomes of a Fiber and an Oil-Seed Variety of Flax". In Genetics and Genomics of Linum, 89–96. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-23964-0_6.

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Stefanova, Isabella, e Veronika Borisova. "Using the Flax Seeds and the Flax Oil in the Production of Chopped Semi-finished Chicken Meat Products in Order to Enrich Them with Polyunsaturated Fatty Acids". In Lecture Notes in Networks and Systems, 191–99. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-96641-6_23.

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ANDERSON, TED L. "Flaw Assessment". In Oil and Gas Pipelines, 579–86. Hoboken, New Jersey: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119019213.ch40.

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Lu, Flora, Gabriela Valdivia e Néstor L. Silva. "Oil Flux and Unrest". In Oil, Revolution, and Indigenous Citizenship in Ecuadorian Amazonia, 265–86. New York: Palgrave Macmillan US, 2016. http://dx.doi.org/10.1057/978-1-137-53362-3_8.

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Baba, Eiichi, Hideo Kawarada, Wataru Nishijima, Mitsumasa Okada e Hiroshi Suito. "Theoretical study of oil pollution". In Waves and Tidal Flat Ecosystems, 71–91. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55534-3_5.

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Baba, Eiichi, Hideo Kawarada, Wataru Nishijima, Mitsumasa Okada e Hiroshi Suito. "Decomposition mechanism of spilled oil by bacteria". In Waves and Tidal Flat Ecosystems, 109–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55534-3_7.

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Bhandari, Amit. "Flux in the energy world". In India and the Changing Geopolitics of Oil, 1–18. London: Routledge India, 2021. http://dx.doi.org/10.4324/9781003152927-1.

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Trabalhos de conferências sobre o assunto "Flax oil"

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Nemirova, L. F., V. L. Shtabnova, S. N. Litunov e N. Yu Filkin. "Experimental studies of flax-containing nonwoven fabric properties as a filter material". In OIL AND GAS ENGINEERING (OGE-2017). Author(s), 2017. http://dx.doi.org/10.1063/1.4998867.

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Ghasemi, Pooya. "Development Of Raw And Treated Flax Straw For Oil Spill Cleanup". In 2018 Canadian Society for Mechanical Engineering (CSME) International Congress. York University Libraries, 2018. http://dx.doi.org/10.25071/10315/35415.

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Belopukhov, S. L., e I. I. Dmitrevskaya. "Dynamics of growth and development of oil flax with the action of protective-stimulating complexes". In Agrobiotechnology-2021. Publishing house RGAU-MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1855-3-2021-96.

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The article studied the effect of pre-sowing treatment of seeds and plants of oil flax varieties Istok and Severny in the “herringbone” phase with the biological product Floravit®. The effective concentration of the drug is 0.1%, which accelerates the dynamics of growth and development of flax; the Istok variety is more responsive to the action.
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Brazhnikov, V. N., O. F. Brazhnikova e D. V. Brazhnikov. "Influence of agroclimatic conditions on yield and fatty acid composition of oil flax". In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2019. http://dx.doi.org/10.33952/09.09.2019.66.

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Belopukhov, S. L. "Prospects for growing flax in the conditions of organic agriculture". In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-93.

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The article provides data on the prospective development of flax cultivation in organic agriculture. It has been shown that organic flaxseed oil grown by technology has a unique set of chemical indicators for organic acids, which makes it possible to identify this type of product and the region of origin. Flax oil and seeds meet the requirements of Russian and international quality standards for this type of product.
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Gongalo, A. A., E. N. Turin, K. G. Zhenchenko, V. Yu Ivanov e T. N. Melnichuk. "Changes in the phytosanitary conditions in case of oil flax growing using microbial preparations". In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.07.

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Over three years of research, the number of weeds per 1 m2 in the direct seeding (DS) variant was 19 pcs. more. At the initial stage of the oil flax development, which was cultivated under conventional technology, the number of unwanted plants in the variant without inoculation was, on average, 32 pcs./m2; with complex microbial preparation (CMP) treatment – 31.5 pcs./m2. In the context of DS technology, there were 51 pcs./m2 in the variant with inoculation; 50 pcs./m2 – without CMP treatment. The increase in the weed component under DS is due to better moisture and nutrients availability. In the experimental plots with and without inoculation, the difference in the variants cannot be proved from the mathematical point of view. Before harvesting, as a result of the herbicidal treatment, the difference between the technologies levelled. The total number of weeds per 1 m2 under conventional technology with the application of CMP was 24 pcs./m2, its average weight – 34.6 g/m2; in the variant without CMP application – 22.4 pcs./m2 and 34 g/m2, respectively. Under DS, with CMP – 21.3 pcs./m2 and 41.2 g/m2; without CMP treatment – 21 pcs./m2 and 41 g/m2. Furthermore, the species composition of weeds did not differ depending on the cultivation technology. Their number, yet, was greater under DS in the stage of full emergence. In the full ripeness stage, the amount of the weed component decreased. At the time of harvesting, it caused no significant effect on the yield neither under DS nor conventional technology regardless of the CMP treatment. However, the weight of dry weeds was higher under DS, on average, by 7 g/m2 per three years.
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"Polymorphisms in FAD3A and FAD3B genes that determine the fatty acid composition of flax oil". In SYSTEMS BIOLOGY AND BIOINFORMATICS (SBB-2020). Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences., 2020. http://dx.doi.org/10.18699/sbb-2020-30.

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Korepanova, Elena, Vera Goreeva, Ramis Galiev e Ildus Fatihov. "Mineral fertilizers and insecticides in the formation of seed yield of the oil flax varieties". In Proceedings of the International Scientific and Practical Conference “Digital agriculture - development strategy” (ISPC 2019). Paris, France: Atlantis Press, 2019. http://dx.doi.org/10.2991/ispc-19.2019.59.

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Akanova, N. I., M. M. Vizirskaya e L. P. Beltyukov. "Effect of phosphogypsum on the fertility of chernozem and productivity of oil flax and winter wheat". In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-1.

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Introduction of 5.0 t/ha of phosphogypsum contributed to the receipt of 1.69 t/ha of oil flax seeds; yield increase was 0.36 tons per hectare or 27%. The content and collection of oil also reliably increased. Winter wheat grain yield in the variant with phosphogypsum was 5.48 tons per hectare; yield increase, in this case, reached 0.74 tons per hectare or 15.6%. Harvest index (HI) of winter wheat in the trial fields with phosphogypsum was 50.0%; the same in the control variant (without phosphogypsum) – 38.0%.
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Melnichuk, T. N., A. A. Gongalo, A. Yu Egovtseva, E. R. Abdurashytova e E. N. Turin. "Impact of microbial preparations on the activity of rhizosphere and the productivity of oil flax under no-till". In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.16.

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Microbial preparations improve mineral nutrition of plants, protect against phytopathogens, and increase their resistance to stress factors. The aim of our research is to study the effect of microbial preparations on the biological activity of rhizosphere and the productivity of oil flax under no-till in the Crimean Steppe. Microbiological analysis of the rhizosphere of oil flax showed that there is a tendency to increase the number of microorganisms of various ecological and trophic groups both under the conditions of the conventional farming system (CFS) and no-till when seeds are inoculated with a complex of microbial preparations (CMP). Under CFS, the number of microorganisms using mineral forms of nitrogen as nutrition increased by 28 %; pedotrophs – by 37 %; ammonifiers and oligotrophs increased under both farming systems. The total number of nitrogen fixers increased by 29 % under CFS as a result of biological preparations use, while under no-till there was only a trend towards increasing the amount of azotobacter. The number of actinomycetes increased under the influence of CMP by 50% under direct sowing; micromycetes decreased under both farming systems. The number of cellulose-degrading microorganisms increased by 18 and 27 % under no- till and CFS, respectively. The yield of oilseed flax under no-till was 0.11 t/ha (12.9 %) higher than under conventional farming system. On average, over three years (2017-2019), an increase in yield amounted to 0.12 t/ha (19%) due to the use of microbial preparations.
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Relatórios de organizações sobre o assunto "Flax oil"

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Eric H. Johnson e Don E. French. INCREASING OIL RECOVERY THROUGH ADVANCED REPROCESSING OF 3D SEISMIC, GRANT CANYON AND BACON FLAT FIELDS, NYE COUNTY, NEVADA. Office of Scientific and Technical Information (OSTI), junho de 2001. http://dx.doi.org/10.2172/828451.

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Hulen, J. B. Investigation of high-temperature, igneous-related hydraulic fracturing as a reservoir control in the Blackburn and Grant Canyon/Bacon Flat oil fields, Nevada. Office of Scientific and Technical Information (OSTI), janeiro de 1991. http://dx.doi.org/10.2172/5790120.

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Olsson, Olle. Industrial decarbonization done right: identifying success factors for well-functioning permitting processes. Stockholm Environment Institute, novembro de 2021. http://dx.doi.org/10.51414/sei2021.034.

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1 Introduction 1.1 The urgency of industrial decarbonization The last few years have seen several of the world’s largest carbon dioxide-emitting countries and leading heavy industry companies committing to mid-century net-zero targets (Buckley 2021; Denyer and Kashiwagi 2020; McCurry 2020; Myers 2020). Consequently, the discussion on economy-wide transition to net-zero is accelerating, with focus shifting from “if” to “when” and “how”, even for heavy industry sectors like steel, cement and chemicals. This makes it increasingly urgent to analyse not just whether it is technologically feasible to decarbonize heavy industry, but also investigate issues more directly related to practical implementation. This includes site-specific planning, infrastructure availability, and consultation with local authorities and other stakeholders. Many of the latter considerations are formalized as part of the permitting processes that are an essential vehicle to ensure that industrial interests are balanced against interests of society at large. However, doing this balancing act can turn out to be very complicated and associated with uncertainties as to their outcome, as well as being demanding in resources and time. At the same time, to ensure broad buy-in and support from society, the investments needed must be implemented in a way that takes a broad spectrum of sustainability concerns into account, not just climate change mitigation. A key question is if and how permitting processes can run more smoothly and efficiently while still ensuring inclusive consultations, fair procedures and adherence to legal certainty. This policy brief discusses this question from the starting point of Swedish conditions, but many of the points raised will be relevant for a broader international discussion on taking industrial decarbonization to implementation. 1.2 Industrial transition and permitting processes in Sweden Decarbonization of the industrial sector in Sweden essentially entails a relatively small number of investment projects in the cement, steel, petrochemical and refinery sectors, where the vast majority of carbon emissions are concentrated (Karltorp et al. 2019; Nykvist et al. 2020). However, while few in number, the size of these investments means that their implementation will by necessity become relevant to many other parts of society. In connection with the increasing focus on how to implement industrial decarbonization in Sweden, discussions about permitting processes have been brought higher up on the agenda. While there has been an active discussion on permitting processes in Sweden for quite some time, it has primarily been focused on aspects related to mining and wind power (Larsen et al. 2017; Raitio et al. 2020). The last few years have, however, focused increasingly on industrial projects, in particular related to a proposed – though eventually cancelled – expansion of an oil refinery in the southwestern part of the country (Blad 2020). In terms of political discussions, both the governmental initiative Fossil-free Sweden (2020) and the Swedish Climate Policy Council (2020) emphasize that permitting processes need to become faster in order for Sweden’s industrial transition to be implemented in line with the time plan set by the 2017 Swedish Climate Act. Business representatives and organizations are also voicing concerns about the slow speed of permitting (Balanskommissionen 2019; Jacke 2018). At the same time, criticism has been raised that much of the environmental damage done in Sweden comes from activities conducted within limits set by environmental permits, which could be a flaw in the system (Malmaeus and Lindblom 2019). Finally, recent public inquiries have also discussed permitting processes.
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Altstein, Miriam, e Ronald J. Nachman. Rational Design of Insect Control Agent Prototypes Based on Pyrokinin/PBAN Neuropeptide Antagonists. United States Department of Agriculture, agosto de 2013. http://dx.doi.org/10.32747/2013.7593398.bard.

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The general objective of this study was to develop rationally designed mimetic antagonists (and agonists) of the PK/PBAN Np class with enhanced bio-stability and bioavailability as prototypes for effective and environmentally friendly pest insect management agents. The PK/PBAN family is a multifunctional group of Nps that mediates key functions in insects (sex pheromone biosynthesis, cuticular melanization, myotropic activity, diapause and pupal development) and is, therefore, of high scientific and applied interest. The objectives of the current study were: (i) to identify an antagonist biophores (ii) to develop an arsenal of amphiphilic topically active PK/PBAN antagonists with an array of different time-release profiles based on the previously developed prototype analog; (iii) to develop rationally designed non-peptide SMLs based on the antagonist biophore determined in (i) and evaluate them in cloned receptor microplate binding assays and by pheromonotropic, melanotropic and pupariation in vivo assays. (iv) to clone PK/PBAN receptors (PK/PBAN-Rs) for further understanding of receptor-ligand interactions; (v) to develop microplate binding assays for screening the above SMLs. In the course of the granting period A series of amphiphilic PK/PBAN analogs based on a linear lead antagonist from the previous BARD grant was synthesized that incorporated a diverse array of hydrophobic groups (HR-Suc-A[dF]PRLa). Others were synthesized via the attachment of polyethylene glycol (PEG) polymers. A hydrophobic, biostablePK/PBAN/DH analog DH-2Abf-K prevented the onset of the protective state of diapause in H. zea pupae [EC50=7 pmol/larva] following injection into the preceding larval stage. It effectively induces the crop pest to commit a form of ‘ecological suicide’. Evaluation of a set of amphiphilic PK analogs with a diverse array of hydrophobic groups of the formula HR-Suc-FTPRLa led to the identification of analog T-63 (HR=Decyl) that increased the extent of diapause termination by a factor of 70% when applied topically to newly emerged pupae. Another biostablePK analog PK-Oic-1 featured anti-feedant and aphicidal properties that matched the potency of some commercial aphicides. Native PK showed no significant activity. The aphicidal effects were blocked by a new PEGylated PK antagonist analog PK-dF-PEG4, suggesting that the activity is mediated by a PK/PBAN receptor and therefore indicative of a novel and selective mode-of-action. Using a novel transPro mimetic motif (dihydroimidazole; ‘Jones’) developed in previous BARD-sponsored work, the first antagonist for the diapause hormone (DH), DH-Jo, was developed and shown to block over 50% of H. zea pupal diapause termination activity of native DH. This novel antagonist development strategy may be applicable to other invertebrate and vertebrate hormones that feature a transPro in the active core. The research identifies a critical component of the antagonist biophore for this PK/PBAN receptor subtype, i.e. a trans-oriented Pro. Additional work led to the molecular cloning and functional characterization of the DH receptor from H. zea, allowing for the discovery of three other DH antagonist analogs: Drosophila ETH, a β-AA analog, and a dF analog. The receptor experiments identified an agonist (DH-2Abf-dA) with a maximal response greater than native DH. ‘Deconvolution’ of a rationally-designed nonpeptide heterocyclic combinatorial library with a cyclic bis-guanidino (BG) scaffold led to discovery of several members that elicited activity in a pupariation acceleration assay, and one that also showed activity in an H. zea diapause termination assay, eliciting a maximal response of 90%. Molecular cloning and functional characterization of a CAP2b antidiuretic receptor from the kissing bug (R. prolixus) as well as the first CAP2b and PK receptors from a tick was also achieved. Notably, the PK/PBAN-like receptor from the cattle fever tick is unique among known PK/PBAN and CAP2b receptors in that it can interact with both ligand types, providing further evidence for an evolutionary relationship between these two NP families. In the course of the granting period we also managed to clone the PK/PBAN-R of H. peltigera, to express it and the S. littoralis-R Sf-9 cells and to evaluate their interaction with a variety of PK/PBAN ligands. In addition, three functional microplate assays in a HTS format have been developed: a cell-membrane competitive ligand binding assay; a Ca flux assay and a whole cell cAMP ELISA. The Ca flux assay has been used for receptor characterization due to its extremely high sensitivity. Computer homology studies were carried out to predict both receptor’s SAR and based on this analysis 8 mutants have been generated. The bioavailability of small linear antagonistic peptides has been evaluated and was found to be highly effective as sex pheromone biosynthesis inhibitors. The activity of 11 new amphiphilic analogs has also been evaluated. Unfortunately, due to a problem with the Heliothis moth colony we were unable to select those with pheromonotropic antagonistic activity and further check their bioavailability. Six peptides exhibited some melanotropic antagonistic activity but due to the low inhibitory effect the peptides were not further tested for bioavailability in S. littoralis larvae. Despite the fact that no new antagonistic peptides were discovered in the course of this granting period the results contribute to a better understanding of the interaction of the PK/PBAN family of Nps with their receptors, provided several HT assays for screening of libraries of various origin for presence of PK/PBAN-Ragonists and antagonists and provided important practical information for the further design of new, peptide-based insecticide prototypes aimed at the disruption of key neuroendocrine physiological functions in pest insects.
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