Artigos de revistas: "Immunohistochemistry"

1

B. Ingle, Sachin. "Immunohistochemistry". International Journal of Current Research and Review 10, n.º 11 (2018): 0–1. http://dx.doi.org/10.31782/ijcrr.2018.10115.

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Taylor, Clive R. "Immunohistochemistry". Applied Immunohistochemistry & Molecular Morphology 27, n.º 5 (2019): 325–26. http://dx.doi.org/10.1097/pai.0000000000000770.

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Mani, Haresh, e Dani S. Zander. "Immunohistochemistry". Chest 142, n.º 5 (novembro de 2012): 1324–33. http://dx.doi.org/10.1378/chest.12-0123.

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4

&NA;. "Immunohistochemistry". Pathology 22 (1990): 9–10. http://dx.doi.org/10.3109/00313029009060103.

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Aryal, G. "Immunohistochemistry". Journal of Pathology of Nepal 5, n.º 10 (14 de setembro de 2015): I. http://dx.doi.org/10.3126/jpn.v5i10.15660.

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Immunohistochemistry (IHC) or immunocytochemistry is a method of localizing specific antigen in tissue or cells based on antigen antibody reaction. IHC is the way of validating morphological findings. It helps in tumor diagnosis and classification, identify prognostic and predictive markers. IHC has a long history that dates back more than 70 years, when Coons1 first developed immunofluroscence technique to detect corresponding antigen in frozen tissue section. At oxford, Taylor and Burns2 developed the first successful demonstration of antigens in routinely processed formalin fixed paraffin-embedded sections. Since the early 1990s, IHC has been applied in routine formalin fixed paraffin embedded tissue. 3-4 Validation of reagents, protocols, controls and staining results are vital steps of IHC. The basic principles and protocols for fresh-frozen tissue sections are the same as those for paraffin sections, except that the antigen retrieval and dewaxing procedures are not required for frozen tissue sections. Titrations may also differ and must be separately optimized. Basic principles: Antigen-antibody recognition is based on the three-dimensional (3D) structure of protein or some other antigen, which may be compromised by formalin-induced modification of protein conformation (“masking”) but is restored in part by Antigen retrieval. Anti-A antibody binds specifically to antigen A in the tissue section. Antigen B (B) is depicted as a second antigenic determinant that is part of the anti-A molecule; anti-B antibody, made in a second species, will bind to this determinant. Thus anti-B, the so-called secondary antibody, can be used to locate the site of binding of anti-A, the primary antibody, in a tissue section. Basic IHC procedure Antigen retrieval (AR)-Enzymatic digestion (proteinase or trypsin), heat treatment (Microwave, water bath or autoclave) Blocking of non-specific background staining Incubation with primary antibody in humidity chamber Add avidin-biotin-peroxidase complex, which binds to secondary antibody Add 3, 3’ diaminobenzidine (DAB) as a chromagen (color changing reagent), with hematoxylin (Mayer's) counterstaining Theranostic Application IHC is becoming increasingly important for the evaluation of predictive markers that can help select patients who may respond to particular targeted therapies. Some of these CD117 for GI stromal tumors, Herceptin (Genentech, South San Francisco, CA) for HER2-positive breast cancers, and rituximab for CD20-positive lymphomas.

6

Dabbs, David J. "Immunohistochemistry". Pathology Case Reviews 4, n.º 6 (novembro de 1999): 229. http://dx.doi.org/10.1097/00132583-199911000-00001.

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Mani, Haresh, e Dani S. Zander. "Immunohistochemistry". Chest 142, n.º 5 (novembro de 2012): 1316–23. http://dx.doi.org/10.1378/chest.11-3327.

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Mazroa, Shireen A. "Immunohistochemistry". Egyptian Journal of Histology 35, n.º 2 (junho de 2012): 191–97. http://dx.doi.org/10.1097/01.ehx.0000414291.44156.ef.

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Wittekind, Christian, e Andrea Tannapfel. "Immunohistochemistry". Digestion 58, n.º 1 (1997): 79–81. http://dx.doi.org/10.1159/000201534.

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Sompuram, Seshi R., Kodela Vani, Brian Tracey, Debra A. Kamstock e Steven A. Bogen. "Standardizing Immunohistochemistry". Journal of Histochemistry & Cytochemistry 63, n.º 9 (4 de maio de 2015): 681–90. http://dx.doi.org/10.1369/0022155415588109.

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Ward, J. M., e J. E. Rehg. "Rodent Immunohistochemistry". Veterinary Pathology 51, n.º 1 (27 de setembro de 2013): 88–101. http://dx.doi.org/10.1177/0300985813503571.

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Cotta, Claudiu V. "Modern Immunohistochemistry". American Journal of Surgical Pathology 35, n.º 6 (junho de 2011): 936. http://dx.doi.org/10.1097/pas.0b013e31821899d9.

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Robboy, Stanley J. "Diagnostic Immunohistochemistry". International Journal of Gynecological Pathology 21, n.º 3 (julho de 2002): 309–10. http://dx.doi.org/10.1097/00004347-200207000-00021.

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Stewart, J. A. "Immunohistochemistry II". Histopathology 25, n.º 3 (setembro de 1994): 294–95. http://dx.doi.org/10.1111/j.1365-2559.1994.tb01337.x.

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Zhang, Paul J. "Diagnostic Immunohistochemistry". Human Pathology 33, n.º 10 (outubro de 2002): 1055. http://dx.doi.org/10.1016/s0046-8177(02)70050-6.

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Fritz, Peter, Hans-Volker Tuczek, Hinke Multhaupt, Joachim Hoenes, Dagmar Lutz, Rainer Doerrer e Peter Schwarzmann. "Quantitative Immunohistochemistry". Progress in Histochemistry and Cytochemistry 24, n.º 3 (janeiro de 1992): III—53. http://dx.doi.org/10.1016/s0079-6336(11)80214-7.

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Li, Huiyan, Gabrielle Brewer, Grant Ongo, Frederic Normandeau, Atilla Omeroglu e David Juncker. "Immunohistochemistry Microarrays". Analytical Chemistry 89, n.º 17 (23 de agosto de 2017): 8620–25. http://dx.doi.org/10.1021/acs.analchem.7b00807.

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18

Kumararante, D. S. "Immunohistochemistry II". Immunology Today 15, n.º 4 (abril de 1994): 195. http://dx.doi.org/10.1016/0167-5699(94)90320-4.

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Chessa, Daniela, Paola Delaconi, Nikki Kelvin, Franco Campus, Luca Sanna, Maria A. Demurtas, David Kelvin, Salvatore Rubino e Vittorio Mazzarello. "Fluorescent Immunohistochemistry". Applied Immunohistochemistry & Molecular Morphology 25, n.º 4 (abril de 2017): 289–97. http://dx.doi.org/10.1097/pai.0000000000000302.

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Handra-Luca, Adriana. "ERG1 Immunohistochemistry". Applied Immunohistochemistry & Molecular Morphology 27, n.º 2 (fevereiro de 2019): e20-e21. http://dx.doi.org/10.1097/pai.0000000000000578.

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Lillemoe, Tamera J. "Diagnostic immunohistochemistry". Diagnostic Cytopathology 27, n.º 5 (novembro de 2002): 316. http://dx.doi.org/10.1002/dc.10223.

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Gown, A. M. "Genogenic immunohistochemistry: a new era in diagnostic immunohistochemistry". Current Diagnostic Pathology 8, n.º 3 (junho de 2002): 193–200. http://dx.doi.org/10.1054/cdip.2002.0116.

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Kaliyappan, Karunakaran, Murugesan Palanisamy, Jeyapradha Duraiyan e Rajeshwar Govindarajan. "Applications of immunohistochemistry". Journal of Pharmacy and Bioallied Sciences 4, n.º 6 (2012): 307. http://dx.doi.org/10.4103/0975-7406.100281.

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24

Ferringer, Tammie. "Immunohistochemistry in Dermatopathology". Archives of Pathology & Laboratory Medicine 139, n.º 1 (1 de janeiro de 2015): 83–105. http://dx.doi.org/10.5858/arpa.2014-0075-ra.

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Context Immunohistochemistry is not a diagnostic test but a highly valuable tool that requires interpretation within a context. Objective To review the current status and limitations of immunohistochemistry in dermatopathology. Data Sources English-language literature published between 1980 and 2014. Conclusions Although immunohistochemistry is rarely completely specific or sensitive, it is an important adjunctive technique in dermatopathology and can be helpful in a series of diagnostic dilemmas.

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Wanstrup, John, e Preben Elling. "IMMUNOHISTOCHEMISTRY OF SARCOIDOSIS". Acta Pathologica Microbiologica Scandinavica 73, n.º 1 (18 de agosto de 2009): 37–48. http://dx.doi.org/10.1111/j.1699-0463.1968.tb00477.x.

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Hewitt, Stephen M., Denis G. Baskin, Charles W. Frevert, William L. Stahl e Eduardo Rosa-Molinar. "Controls for Immunohistochemistry". Journal of Histochemistry & Cytochemistry 62, n.º 10 (14 de julho de 2014): 693–97. http://dx.doi.org/10.1369/0022155414545224.

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27

Shi, Shan-Rong, Yan Shi e Clive R. Taylor. "Antigen Retrieval Immunohistochemistry". Journal of Histochemistry & Cytochemistry 59, n.º 1 (janeiro de 2011): 13–32. http://dx.doi.org/10.1369/jhc.2010.957191.

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28

Rimm, David L. "Next-gen immunohistochemistry". Nature Methods 11, n.º 4 (28 de março de 2014): 381–83. http://dx.doi.org/10.1038/nmeth.2896.

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Fuertes, Laura, Carlos Santonja, Heinz Kutzner e Luis Requena. "Immunohistochemistry in Dermatopathology". American Journal of Dermatopathology 38, n.º 2 (fevereiro de 2016): 92–104. http://dx.doi.org/10.1097/dad.0000000000000361.

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Nayar, Ritu, e Sana O. Tabbara. "DetectingPneumocystis cariniiby Immunohistochemistry". Laboratory Medicine 27, n.º 8 (1 de agosto de 1996): 547–50. http://dx.doi.org/10.1093/labmed/27.8.547.

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31

Mao, Tsui-Lien, Robert J. Kurman, Chao-Cheng Huang, Ming-Chieh Lin e Ie-Ming Shih. "Immunohistochemistry of Choriocarcinoma". American Journal of Surgical Pathology 31, n.º 11 (novembro de 2007): 1726–32. http://dx.doi.org/10.1097/pas.0b013e318058a529.

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32

Ruegg, Patsy. "Immunohistochemistry Internet Resources". Journal of Histotechnology 25, n.º 4 (dezembro de 2002): 267–68. http://dx.doi.org/10.1179/his.2002.25.4.267.

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33

O'Leary, Timothy J. "Standardization in Immunohistochemistry". Applied Immunohistochemistry & Molecular Morphology 9, n.º 1 (março de 2001): 3–8. http://dx.doi.org/10.1097/00022744-200103000-00002.

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34

DeLellis, Ronald A. "Advances in Immunohistochemistry". American Journal of Surgical Pathology 12, n.º 12 (dezembro de 1988): 972. http://dx.doi.org/10.1097/00000478-198812000-00017.

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35

Schnitt, Stuart J. "Advances in Immunohistochemistry". American Journal of Surgical Pathology 13, n.º 5 (maio de 1989): 436. http://dx.doi.org/10.1097/00000478-198905000-00021.

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36

&NA;. "Immunohistochemistry Study Group". American Journal of Surgical Pathology 15, n.º 9 (setembro de 1991): 914. http://dx.doi.org/10.1097/00000478-199109000-00021.

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37

Renshaw, Andrew A., e Christopher L. Corless. "Histology and Immunohistochemistry". American Journal of Surgical Pathology 19, n.º 7 (julho de 1995): 842–49. http://dx.doi.org/10.1097/00000478-199507000-00013.

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38

Kapur, Raj P., Philipp W. Raess, Samuel Hwang e Conrad Winter. "Choline Transporter Immunohistochemistry". Pediatric and Developmental Pathology 20, n.º 4 (23 de março de 2017): 308–20. http://dx.doi.org/10.1177/1093526617697060.

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Acetylcholinesterase enzymatic histochemistry (AChE EHC), which highlights abnormal cholinergic nerves in the mucosa of aganglionic bowel, has been used for decades to evaluate rectal biopsies for Hirschsprung disease (HSCR). While useful diagnostically, AChE EHC is not compatible with conventional formalin-fixed and paraffin-embedded (FFPE) tissues and is not widely available. The choline transporter (ChT) is a putative alternative marker of cholinergic nerves. ChT immunohistochemistry (IHC) was investigated using FFPE biopsies and resections from patients with confirmed HSCR, as well as appropriate non-HSCR controls. ChT immunostaining was effective at identifying cases with HSCR and qualitatively similar to AChE EHC on frozen section. Among 3 pathologists, the diagnostic positive and negative predictive values based on ChT IHC ranged from 0.84–0.94 and 0.85–0.89, respectively, with good inter-observer agreement (Cohen kappa = 0.70–0.90). ChT IHC was useful in unusual scenarios in which calretinin (CR) IHC failed to correctly identify patients with HSCR. In 10 cases of short-segment HSCR, abnormal ChT+ mucosal innervation was present through the entire aganglionic segment and into portions of the TZ with submucosal nerve hypertrophy. In contrast, mucosal CR IHC was retained in the TZ and adjacent aganglionic bowel, which could lead to misinterpretation of a biopsy as ganglionic bowel. Indeed, 6 such patients were identified with paradoxical CR-positive mucosal innervation in their diagnostic biopsies. ChT IHC was interpreted as unequivocal HSCR in these cases, and HSCR was confirmed on resection. In summary, ChT IHC in FFPE tissue demonstrates high positive and negative predictive values for HSCR, is superior to CR IHC in a subset of cases, and can be incorporated into routine practice without the need for specialized techniques.

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Kubier, Patty, e Rodney T. Miller. "Tissue Protection Immunohistochemistry". American Journal of Clinical Pathology 117, n.º 2 (fevereiro de 2002): 194–98. http://dx.doi.org/10.1309/hgc2-rjtq-1t7m-19ju.

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40

O'Leary, Timothy J. "Standardization in Immunohistochemistry". Applied Immunohistochemistry & Molecular Morphology 9, n.º 1 (março de 2001): 3–8. http://dx.doi.org/10.1097/00129039-200103000-00002.

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41

Alvaro, T., M. Gomez-Morales, D. Aguilar e J. Aneiros. "Immunohistochemistry of meningiomas". Histopathology 15, n.º 3 (setembro de 1989): 316–17. http://dx.doi.org/10.1111/j.1365-2559.1989.tb03092.x.

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42

Podkletnova, I., e H. Alho. "Ultrasound-amplified immunohistochemistry." Journal of Histochemistry & Cytochemistry 41, n.º 1 (janeiro de 1993): 51–56. http://dx.doi.org/10.1177/41.1.8417112.

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We describe a novel technique for improving the sensitivity of immunofluorescence staining by use of ultrasonic irradiation. Free-floating vibratome sections from rat cerebellum were incubated with primary antiserum and simultaneously were briefly exposed to ultrasound (US) in a conventional ultrasound bath. After the US treatment, a conventional immunohistochemical method was employed. Two different antisera and two conventional immunohistochemical detection systems were tested. In all cases a 10-20-sec US treatment strengthened immunoreactivity considerably. Irradiated samples showed a good morphology compared with non-irradiated sections. Our results demonstrate that with ultrasonic treatment the dilutions of primary antibodies can be increased and the incubation times of primary antisera can be reduced. The ultrasonic method described here requires no special equipment. It is easy, reproducible, and it can be considered a new method for the enhancement at immunohisto- and cytochemical staining of free-floating vibratome sections.

43

Swanson, Paul E. "Foundations of Immunohistochemistry". American Journal of Clinical Pathology 90, n.º 3 (1 de setembro de 1988): 333–39. http://dx.doi.org/10.1093/ajcp/90.3.333.

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44

Taschini, Pier A., e Doris M. Macdonald. "Ficin in Immunohistochemistry". American Journal of Clinical Pathology 90, n.º 6 (1 de dezembro de 1988): 754. http://dx.doi.org/10.1093/ajcp/90.6.754.

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45

Johnson, Carol W. "Issues in Immunohistochemistry". Toxicologic Pathology 27, n.º 2 (março de 1999): 246–48. http://dx.doi.org/10.1177/019262339902700214.

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46

Rittman, Barry R. J. "Quantitation In Immunohistochemistry". Microscopy Today 6, n.º 8 (outubro de 1998): 8–9. http://dx.doi.org/10.1017/s1551929500069157.

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Valuable information concerning the relative amounts of the end proucts of histochemical and immunochemical reactions present in sections may be provided by qualitative evaluations, however, greater reliance is often placed on quantitative evaluations. Many quantitative evaluations are based on the use of image analysis and optical density readings of the visible end products. An important question is whether these quantitative measurements are reliable, accurate and reproducible, and if quantitation of these reactions offers any real advantage over qualitative evaluations.

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Boon, Mathilde E., e L. P. Kok. "Microwaves for immunohistochemistry". Micron 25, n.º 2 (abril de 1994): 151–70. http://dx.doi.org/10.1016/0968-4328(94)90040-x.

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Wistuba, I., E. Parra e A. Francisco Cruz. "MS17.04 Multiplex Immunohistochemistry". Journal of Thoracic Oncology 14, n.º 10 (outubro de 2019): S191. http://dx.doi.org/10.1016/j.jtho.2019.08.380.

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&NA;. "Immunology And Immunohistochemistry". Pathology 25, Suppl 1 (1993): 9–10. http://dx.doi.org/10.3109/00313029309107644.

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Arnold, Wolfgang, e Imre Friedmann. "Immunohistochemistry of Otosclerosis". Acta Oto-Laryngologica 109, sup470 (1 de janeiro de 1990): 124–29. http://dx.doi.org/10.3109/00016488909138366.

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