Bibliografias temáticas / Imprinted

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Literatura científica selecionada sobre o tema "Imprinted"

Autor: Grafiati

Publicado: 1 de abril de 2023

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Artigos de revistas sobre o assunto "Imprinted"

Arnaud, Philippe. "Genomic imprinting in germ cells: imprints are under control". REPRODUCTION 140, n.º 3 (setembro de 2010): 411–23. http://dx.doi.org/10.1530/rep-10-0173.

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The cis-acting regulatory sequences of imprinted gene loci, called imprinting control regions (ICRs), acquire specific imprint marks in germ cells, including DNA methylation. These epigenetic imprints ensure that imprinted genes are expressed exclusively from either the paternal or the maternal allele in offspring. The last few years have witnessed a rapid increase in studies on how and when ICRs become marked by and subsequently maintain such epigenetic modifications. These novel findings are summarised in this review, which focuses on the germline acquisition of DNA methylation imprints and particularly on the combined role of primary sequence specificity, chromatin configuration, non-histone proteins and transcriptional events.

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Smolinska-Kempisty, Katarzyna, Joanna Wolska e Marek Bryjak. "Molecularly Imprinting Microfiltration Membranes Able to Absorb Diethyl Phthalate from Water". Membranes 12, n.º 5 (8 de maio de 2022): 503. http://dx.doi.org/10.3390/membranes12050503.

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In this study, polypropylene porous membranes with an average pore size of 1.25 µm were modified by barrier discharge plasma. Next, molecularly imprinted layers with an imprint of diethyl phthalate (DEP) ware grafted of their surface. In order to optimize the composition of the modifying mixture various solvents, the ratios of functional monomers and the cross-linking monomer as well as various amounts of phthalate were verified. It was shown that the most effective membranes were obtained during polymerization in n-octane with the participation of functional monomers in the ratio 3:7 and the amount of phthalate 7 wt.%. The membranes were tested in the filtration process as well as static and dynamic sorption. In all of these processes, the imprinted membranes showed better properties than those without the imprint. The diethyl phthalate retention coefficient was 36.12% for membranes with a grafting yield of 1.916 mg/cm2. On the other hand, DEP static sorption for the imprinted membranes was 3.87 µmol/g higher than for non-imprinted membranes. Also, in the process of dynamic sorption higher values were observed for membranes with the imprint (DSMIM, 4.12 µmol/g; DSNIM, 1.18 µmol/g). The membranes were also tested under real conditions. In the process of filtration of tap water contaminated with phthalate, the presence of imprints in the membrane structure resulted in more than three times higher sorption values (3.09 µmol/g) than in the case of non-imprinted membranes (1.12 µmol/g).

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Nykänen, Nooa. "Following the Old Road: Organizational Imprinting and the Regional Development of Russia". Management and Organization Review 17, n.º 3 (19 de maio de 2021): 583–616. http://dx.doi.org/10.1017/mor.2020.83.

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ABSTRACTIn this article, I draw from organizational imprinting theory to illuminate the impact of the Soviet legacy on contemporary Russian economic geography and regional policy. I argue that central coordination in the creation and regulation of Russian urban agglomerations is connected to a socialist imprinted paradigm associated with the Soviet economic regionalization model and territorial-production complexes (TPCs). I conduct a qualitative historical study to analyze the role of the foundational environment and the dynamics in the development of this imprint. I propose that this imprint effect is prone to reproduction in contemporary regional development strategies and community-based paradigms due to exaptation and cultural-cognitive persistence. The article extends the literature of socialist imprinting by demonstrating how imprints may emerge, transform, and affect localized organizational communities in transition economies and highlights the role of imprinted paradigms in policymaking and regional development.

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O'Doherty, A. M., D. Magee, M. E. Beltman, S. Mamo, D. Rizos e T. Fair. "88 VARIABLE DNA METHYLATION PROFILES AT IMPRINTED LOCI IN BOVINE EARLY PRE-IMPLANTATION EMBRYOS". Reproduction, Fertility and Development 25, n.º 1 (2013): 192. http://dx.doi.org/10.1071/rdv25n1ab88.

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The DNA methylation imprints, at maternally imprinted gene differentially methylated regions, are established during the postnatal growth stage of oogenesis, with paternal imprints being acquired in the perinatal prospermatagonia. Murine DNA methylation marks, at imprinted loci, are widely regarded to be resistant to post-fertilization demethylation events that occur in the paternal pronucleus of the zygote and to passive demethylation of the maternally derived genomic content from cleavage to the 16-cell stage. However, the DNA methylation profile of bovine imprinted genes following fertilization remains unknown. The objective of the current study was to analyze the methylation dynamics at several imprinted gene differentially methylated regions during bovine embryo development. In addition, a previously published RNA-seq database (Mamo et al. 2011 Biol. Reprod.) was mined for transcript abundance of genes associated with establishing and maintaining genomic imprints. Single in vivo blastocysts (Day 7), hatched ovoid embryos (Day 14), filamentous embryos (Day 17), and implanting conceptii (Day 25) were collected (n = 4–9, per time point) from beef heifers. Genomic DNA was isolated and bisulfite modified, using the EZ DNA methylation direct kit (Zymo, Irvine, CA, USA), and used as template in bisulfite PCR reactions. The PCR products were verified by agarose gel electrophoresis and subsequently pyrosequenced. Observed methylation values were most highly variable in Day 7 blastocysts, with values ranging between 13 and 44% (IGF2R), 5 and 63% (PEG10), 7 and 59% (MEST), 3 and 61% (SNRPN), 12 and 64% (PLAGL1), and 20 and 32% (H19). There was a marked reduction in variability as embryonic development progressed, with values at Day 25 ranging from 37 to 41% (IGF2R), 34 to 38% (PEG10), 31 to 37% (MEST), 36 to 40% (SNRPN), 17 to 26% (PLAGL1), and 25 to 30% (H19). Statistical analysis (Levene’s test for equal variance) of methylation values for each gene at each time point confirmed that the methylation values observed in Day 7 embryos were significantly variable (P < 0.05) when compared with later developmental stages. Concordant with this finding, RNA transcript levels of associated methylation machinery genes DNMT3A, DNMT3B, and TRIM28 progressively increased from Day 7 to 13 and subsequently decreased from Day 13 to 16. Taken together our results demonstrate that in cattle DNA methylation marks, at imprinted loci, are highly variable at the blastocyst stage and are progressively stabilized with increasing days post-fertilization. This stabilization of imprint is coordinated with a window of increased levels of associated methylation machinery transcripts. Work presented here provides evidence of a novel mechanism for bovine embryonic DNA methylation imprint maintenance. This work was funded by SFI grant number 07/SRC/B1156.

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Kato, Y., W. M. Rideout, K. Hilton, S. C. Barton, Y. Tsunoda e M. A. Surani. "Developmental potential of mouse primordial germ cells". Development 126, n.º 9 (1 de maio de 1999): 1823–32. http://dx.doi.org/10.1242/dev.126.9.1823.

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There are distinctive and characteristic genomic modifications in primordial germ cells that distinguish the germ cell lineage from somatic cells. These modifications include, genome-wide demethylation, erasure of allele-specific methylation associated with imprinted genes, and the re-activation of the X chromosome. The allele-specific differential methylation is involved in regulating the monoallelic expression, and thus the gene dosage, of imprinted genes, which underlies functional differences between parental genomes. However, when the imprints are erased in the germ line, the parental genomes acquire an equivalent epigenetic and functional state. Therefore, one of the reasons why primordial germ cells are unique is because this is the only time in mammals when the distinction between parental genomes ceases to exist. To test how the potentially imprint-free primordial germ cell nuclei affect embryonic development, we transplanted them into enucleated oocytes. Here we show that the reconstituted oocyte developed to day 9.5 of gestation, consistently as a small embryo and a characteristic abnormal placenta. The embryo proper also did not progress much further even when the inner cell mass was ‘rescued’ from the abnormal placenta by transfer into a tetraploid host blastocyst. We found that development of the experimental conceptus was affected, at least in part, by a lack of gametic imprints, as judged by DNA methylation and expression analysis of several imprinted genes. The evidence suggests that gametic imprints are essential for normal development, and that they can neither be initiated nor erased in mature oocytes; these properties are unique to the developing germ line.

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Vu, Hoang Yen, e A. N. Zyablov. "Determination of preservatives in liquids by piezosensors". Аналитика и контроль 26, n.º 2 (2022): 134–40. http://dx.doi.org/10.15826/analitika.2022.26.2.001.

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In the current study, piezosensors based on the molecularly imprinted polyimides with imprints of potassium sorbate and sodium benzoate were obtained. Molecularly Imprinted Polymers (MIPs) were synthesized using a polyimide and a non-covalent imprinting technique. It was established that the use of 0.1 g/mL template concentration at the thermochemical stage led to the formation of the maximum number of molecular imprints on the film surface. Using the scanning force microscopy, it was found that the reference polymer film had a uniform surface with a small height difference from 1.4 to 2.6 nm (including 88.94 % of pores with a radius of up to 10 nm) and had good film thickness reproducibility. The surface morphology of films of molecularly imprinted polymers with imprints of potassium sorbate and sodium benzoate had more developed surface, which was associated with the peculiarities of the formation of imprints. In this work, imprinting factor (IF) and selectivity coefficient (k) of the sensor were calculated. Molecularly imprinted polymers had better selectivity, sensitivity, and ability to recognize target template molecules than the reference polymers (non-imprinted polymers). Molecularly imprinted polymer-modified piezoelectric sensors (MIP sensors) have been used to analyze potassium sorbate and sodium benzoate in non-alcoholic drinks. The linear concentration range was identified to be 5 - 500 mg/L and the limit of detection for potassium sorbate and sodium benzoate were 1.6 mg/L and 2 mg/L, respectively. The determination of potassium sorbate and sodium benzoate in non-alcoholic drinks was carried out by MIP sensors and spectrophotometry method. The results obtained by the sensors and the spectrophotometry method were in good agreement. The concentration of preservatives for the potassium sorbate and the sodium benzoate in non-alcoholic drinks was 130 - 176 mg/L and 129 - 146 mg/L, respectively.

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Edwards, Carol A., Nozomi Takahashi, Jennifer A. Corish e Anne C. Ferguson-Smith. "The origins of genomic imprinting in mammals". Reproduction, Fertility and Development 31, n.º 7 (2019): 1203. http://dx.doi.org/10.1071/rd18176.

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Genomic imprinting is a process that causes genes to be expressed according to their parental origin. Imprinting appears to have evolved gradually in two of the three mammalian subclasses, with no imprinted genes yet identified in prototheria and only six found to be imprinted in marsupials to date. By interrogating the genomes of eutherian suborders, we determine that imprinting evolved at the majority of eutherian specific genes before the eutherian radiation. Theories considering the evolution of imprinting often relate to resource allocation and recently consider maternal–offspring interactions more generally, which, in marsupials, places a greater emphasis on lactation. In eutherians, the imprint memory is retained at least in part by zinc finger protein 57 (ZFP57), a Kruppel associated box (KRAB) zinc finger protein that binds specifically to methylated imprinting control regions. Some imprints are less dependent on ZFP57invivo and it may be no coincidence that these are the imprints that are found in marsupials. Because marsupials lack ZFP57, this suggests another more ancestral protein evolved to regulate imprints in non-eutherian subclasses, and contributes to imprinting control in eutherians. Hence, understanding the mechanisms acting at imprinting control regions across mammals has the potential to provide valuable insights into our understanding of the origins and evolution of genomic imprinting.

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Tunster, S. J., A. B. Jensen e R. M. John. "Imprinted genes in mouse placental development and the regulation of fetal energy stores". REPRODUCTION 145, n.º 5 (maio de 2013): R117—R137. http://dx.doi.org/10.1530/rep-12-0511.

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Imprinted genes, which are preferentially expressed from one or other parental chromosome as a consequence of epigenetic events in the germline, are known to functionally converge on biological processes that enablein uterodevelopment in mammals. Over 100 imprinted genes have been identified in the mouse, the majority of which are both expressed and imprinted in the placenta. The purpose of this review is to provide a summary of the current knowledge regarding imprinted gene function in the mouse placenta. Few imprinted genes have been assessed with respect to their dosage-related action in the placenta. Nonetheless, current data indicate that imprinted genes converge on two key functions of the placenta, nutrient transport and placental signalling. Murine studies may provide a greater understanding of certain human pathologies, including low birth weight and the programming of metabolic diseases in the adult, and complications of pregnancy, such as pre-eclampsia and gestational diabetes, resulting from fetuses carrying abnormal imprints.

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MacDonald, William A. "Epigenetic Mechanisms of Genomic Imprinting: Common Themes in the Regulation of Imprinted Regions in Mammals, Plants, and Insects". Genetics Research International 2012 (15 de fevereiro de 2012): 1–17. http://dx.doi.org/10.1155/2012/585024.

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Genomic imprinting is a form of epigenetic inheritance whereby the regulation of a gene or chromosomal region is dependent on the sex of the transmitting parent. During gametogenesis, imprinted regions of DNA are differentially marked in accordance to the sex of the parent, resulting in parent-specific expression. While mice are the primary research model used to study genomic imprinting, imprinted regions have been described in a broad variety of organisms, including other mammals, plants, and insects. Each of these organisms employs multiple, interrelated, epigenetic mechanisms to maintain parent-specific expression. While imprinted genes and imprint control regions are often species and locus-specific, the same suites of epigenetic mechanisms are often used to achieve imprinted expression. This review examines some examples of the epigenetic mechanisms responsible for genomic imprinting in mammals, plants, and insects.

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Csaba, György. "Hormonal imprinting in the central nervous system: causes and consequences". Orvosi Hetilap 154, n.º 4 (janeiro de 2013): 128–35. http://dx.doi.org/10.1556/oh.2013.29533.

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The notion of the perinatal „hormonal imprinting” has been published at first in 1980 and since that time it spred expansively. The imprintig develops at the first encounter between the developing receptor and the target hormone – possibly by the alteration of the methylation pattern of DNA – and it is transmitted to the progeny generations of the cell. This is needed for the complete development of the receptor’s binding capacity. However, molecules similar to the target hormone (hormone-analogues, drugs, chemicals, environmental pollutants) can also bind to the developing receptor, causing faulty imprinting with life-long consequences. This can promote pathological conditions. Later it was cleared that in other critical periods such as puberty, imprinting also can be provoked, even in any age in differentiating cells. The central nervous system (brain) also can be mistakenly imprinted, which durably influences the dopaminergic, serotonergic and noradrenergic system and this can be manifested – in animal experiments – in alterations of the sexual and social behavior. In our modern age the faulty hormonal imprintig is inavoidable because of the mass of medicaments, chemicals, the presence of hormone-like materials (e.g. soya phytosteroids) in the food, and environmental pollutants. The author especially emphasizes the danger of oxytocin, as a perinatal imprinter, as it is used very broadly and can basically influence the emotional and social spheres and the appearance of certain diseases such as auitism, schizophrenia and parkinsonism. The danger of perinatal imprinters is growing, considering their effects on the human evolution. Orv. Hetil., 2013, 154, 128–135.

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Mais fontes

Teses / dissertações sobre o assunto "Imprinted"

Robak, Andrew Joseph. "Development of coenzyme-imprinted molecularly imprinted polymers as catalysts /". view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1276397881&sid=1&Fmt=2&clientId=11238&RQT=309&VName=PQD.

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Thesis (Ph. D.)--University of Oregon, 2007.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 94-100). Also available for download via the World Wide Web; free to University of Oregon users.

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Bates, Ferdia. "Design and development of molecularly imprinted polymers and imprinted sensors". Doctoral thesis, Universitat Autònoma de Barcelona, 2016. http://hdl.handle.net/10803/399170.

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Esta tesis se ha hecho principalmente para estudiar e investigar polímeros impresos (MIPs) con la intención de usarlos como sensores de larga vida. La línea de investigación de esta tesis es la dirigida a conseguir la integración de estas formaciones impresas dentro de una lengua electrónica (ET), que es la rama de especialización en la que se ha desarrollado principalmente este proyecto. Después de hacer una revisión de la literatura, que inicialmente se centraba en la aplicación de MIPs a un equipo electroquímico, un sensor voltamétrico impreso y un procedimiento sensitivo complementario, el procedimiento se creó a través de una combinación de protocolos tomados de la literatura. Este sensor, descrito en el Artículo 1, presentaba una buena selectividad hacia el analito primario, teofilina, además de la especificidad requerida frente a sus análogos estructurales. Aunque el diseño del sensor permitía una mejor regeneración de la superficie respecto a otros sistemas parecidos encontrados en la literatura, el comportamiento de los polímeros usados en el MIP retardaba la tasa de transferencia de electrones en la superficie del sensor. Por culpa de este fenómeno, la sensibilidad del sensor se reducía. Justo después de estos experimentos iniciales, se hizo una colaboración con el grupo del Profesor Sergey Piletskey en la Universidad de Leicester (UoL) de Reino Unido. Durante este período, se realizó un estudio intensivo del proceso de diseño de impresión molecular asistido por un sistema computacional de modelización molecular ‘inhouse’. Se puso énfasis en el diseño de un receptor impreso para la molécula de baja solubilidad melanina, que se toma como ‘template modelo’. El MIP resultante se caracterizó y usó para la detección de melanina en muestras de leche, tal y como se describe y detalla en el Artículo 2. Más tarde, utilizando los conocimientos adquirido durante la estancia en Leicester, se desarrollaron nuevas técnicas de modelización computacional para la evaluación de los métodos utilizados en la modelización de MIPs, con el objetivo de obtener una técnica de evaluación virtual para el diseño de receptores impresos, optimizados para los requerimientos necesarios para su posterior aplicación en un sensor ET, tal como se detalla en el Artículo 3. Tal y como se detalla en el capítulo final de esta tesis, la experiencia y conocimientos adquiridos durante la investigación, se usaron para diseñar un grupo de sensores que funcionan asociados a ET. Este desarrollo podría ampliarse profundizando en la selección computacional de polielectrolitos, que luego serían inmovilizados en la superficie de un electrodo voltamétrico mediante una tinta de grafito conductora, de elevada robustez y estabilidad. En este sentido, también se proponen otras recomendaciones para lograr la mejora de la capacidad de regeneración de los MIPs utilizados, por ejemplo mediante la separación de MIP y electrodo. Finalmente, se presentan algunas sugerencias para colaboraciones institucionales, con el propósito de crear un sistema ET móvil, que permita recoger y analizar muestras en campo.
This thesis was predominantly undertaken to study and investigate molecular imprinted polymers (MIPs) with a view to their use as high longevity sensing elements in sensor arrays. The research line of the thesis was intended to lead to the integration of these imprinted arrays into an Electronic Tongue (ET) sensing system which is the area of expertise of the research group in which this project was primarily executed. Having initially executed a review of the literature, focusing initially on the application of the MIPs to an electrochemical device, an imprinted voltammetric sensor and a complimentary sensing procedure was developed using a combination of protocols extracted from the literature. This sensor, described in Article 1, had good selectivity toward the primary analyte, theophylline, and specificity against structural analogues. Though the design of the sensor allowed for significantly improved regeneratibility of the sensor relative to similar systems in the literature, the insulating nature of the polymers used in the MIP reduced the electron transfer rate at the sensor surface and thus resulted in a reduction in sensitivity. Following this initial experimental study, a secondment was undertaken in the University of Leicester under the supervision of Professor Sergey Piletsky. During this period, an intensive study of the design process of molecular imprinting, aided by an in-house computational molecular modelling platform, was conducted focusing on the design of an imprinted receptor for the low solubility 'model template', melamine. This MIP was successfully synthesised, characterised and used in the detection of melamine in milk samples, as detailed in Article 2. Further development of computational modelling techniques for the evaluation of MIP modelling techniques was also achieved with a view to create a virtual evaluation technique for the design of imprinted receptor sites optimised for the requirements of their application to an ET sensor array using the skills acquired during the Leicester secondment as detailed in Article 3. As detailed in the final chapter of this thesis, the insight into the imprinting process which was acquired during the research has been used to design a sensor array system which meets the specifications of ET experimental runs. This takes the form of the introduction of the research topic computationally selected polyelectrolytes, immobilised onto a voltammetric electrode surface via highly robust conducting graphite ink. Additional recommendations are also made to further enhance the on-going MIP projects within the laboratory, such as the separation of the MIP and the electrode to increase MIP regeneratibility. Some final suggestions for some other inter-institutional collaboration are also presented which aim to creating portable ET system for in-field sample collection and analysis.

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Morán, Barroso Verónica Fabiola. "Identification and analysis of imprinted and non-imprinted genes in distal human chromosome 20q13". Thesis, University of Edinburgh, 2001. http://hdl.handle.net/1842/23130.

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This thesis describes work that arose from studies of the imprinting of GNAS1, on human chromosome 20q13. Null mutations in GNAS1 cause the hormone-resistance syndrome pseudohpoparathyroidism type 1a (PHP1a). It was demonstrated that GNAS1 is imprinted, as predicted from the anomalous inheritance of PHP1a, but that its allele-specific regulation is highly complex. This gene is shown to encode several protein products: (i) the alpha subunit of the stimulatory G protein G_s; this protein is biallelically derived; (ii) NESP55, a neuroendocrine secretory protein, expressed exclusively from the maternal allele; (iii) XLas, a Golgi-specific G protein that is expressed exclusively from the paternal allele. Many known imprinted genes lie in clusters that may span several hundred of kb, and may be co-ordinately regulated by a imprinting control region. To assess whether GNAS1 is part of such an imprinted gene cluster, genomic clones were analysed for the presence of other nearby transcripts. This resulted in the identification of two novel genes, CTSZ and CGI107, as well as a number of putative transcripts that lie within GNASl. One of the latter forms part of a spliced polyadenylated antisense transcript that spans the upstream region of NESP55. The CTSZ gene was shown to comprise 6 exons and 5 introns, spanning ~ 12 kb. It encodes cathepsin Z, a cysteine protease whose precise function is undefined. The CG1107 gene was shown to comprise 6 exons and 5 introns, spanning ~ 10 kb, encoding 194 amino acid protein of unknown function, but showing sequence similarity to the kisir protein in Drosophila. Four chromosomally dispersed processed pseudogenes of CG1107 were identified. Together with the biallelic expression of another neighbouring gene TH1, these results suggest the possibility that GNAS1 may not after all be part of an extensive cluster of imprinted genes. This has implications for further studies of the mechanism of imprinting control in this region.

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O'Donnell, Elizabeth Anne. "Water-compatible molecularly imprinted polymers". Thesis, University of Strathclyde, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.438467.

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Canfarotta, Francesco. "Molecularly imprinted nanoparticles for diagnostic applications". Thesis, University of Leicester, 2016. http://hdl.handle.net/2381/37775.

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Molecularly imprinted polymers (MIP) are gaining increasing interest thanks to their low cost of manufacturing, robustness and stability compared to their bio-analogues such as antibodies. The molecular imprinting process can be defined as the generation of molecular recognition sites in a synthetic polymer. The template-derived sites thus created within the polymeric matrix allow MIPs (often referred as plastic antibodies, due to their synthetic nature) to selectively recognise and bind the target molecule. In light of these properties, MIPs have been successfully applied in sensors, assays and separation applications. Due to their small size, MIP nanoparticles (NPs or nanoMIPs) can be used in biomedicine, since the nanoscale format is potentially suitable for cellular or in vivo applications. The aim of this work is to demonstrate the suitability of the nanoMIPs as tools for imaging in cells. For this purpose, the choice of appropriate fluorescent moieties to be included in the nanoMIPs is crucial and depends on the intended application. Several fluorescent monomers were characterised and chosen as imaging functionalities to be employed in the synthesis of MIP NPs (Chapter 2). The innovative solid-phase approach used in this work enables the synthesis of nanoMIPs both in organics (for small templates) and in water (for peptides and proteins), with the possibility to tailor the particle’s surface chemistry according to the intended use. (Chapter 3 and 4). Only few examples of MIP NPs for cellular imaging have been reported so far. Such nanosystems should be biocompatible and physiochemically stable under physiological conditions, as demonstrated in Chapter 5 and 6. Thanks to their good biocompatibility and recognition properties, MIP NPs were successfully applied as membrane-targeted diagnostic tools (Chapter 7) in both cancer and senescent cells, thus paving the way for their in vivo use as diagnostic and imaging tools.

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Bonini, Francesca. "Molecularly imprinted polymers for protome analysis". Thesis, Cranfield University, 2008. http://dspace.lib.cranfield.ac.uk/handle/1826/2716.

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Fast and efficient methods for the detection of insurgence and progression of diseases are at the basis of modern diagnostics and medicine. In this concern, biomarkers represent a powerful diagnostic tool, as their expression profiles well correlate with the pathology progression. Thus, the pathological state could be diagnosed by measuring the altered presence of a biomarker. In this direction, conspicuous help has been given by proteomics, intended as the study of the protein pattern of a sample and most frequently performed by two-dimensional electrophoresis. Although the proteome approach is a powerful analytical method, its application to biological samples for the detection and quantification of putative biomarkers is hampered by technical problems, in fact, the wide diversity in concentrations exhibited by the proteins present in the biological samples, with a concentration range spanning over nine orders of magnitude, and the relative abundance of each protein, are responsible of masking the less abundant species and of their loss in traceability. The aim of my PhD project is to apply Molecularly Imprinted Technology to the specific removal of a high abundance protein (Human Serum Albumin, HSA) frequently affecting proteomic analysis, in order to increase the detection of potential biomarkers. This technology allows the creation of artificial recognition sites in synthetic polymers for a specific protein. These sites are tailor-made in situ by co-polymerisation of functional monomers and cross-linkers around the template molecules. Two different approaches have been assayed in order to remove HSA: • Immobilisation of protein template on a rigid silica support (bead) and creation of polymer around beads. • Polymerisation in bulk of a polymer with protein template and application of this polymer to multicompartment electrolyser. In both of the cases, the chemical and structural features of the polymers have been analysed, after that they have been applied to complex proteome pre-treatment, obtaining encouraging results.

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Mistry, Reena. "Niacinamide analysis using molecularly imprinted polymers". Thesis, University of British Columbia, 2002. http://hdl.handle.net/2429/43182.

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The objectives of this research were to use molecularly imprinted polymers (MIP) and microfluidic chips as an approach to a rapid and low cost analytical method for niacinamide analysis. Lab-on-a-chip (microfluidics) devices are becoming increasingly popular due to their relatively low cost, sensitivity, and speed. MIPs may be able to serve as solid-phase extraction packing material in microfluidic chips. To reach the objectives, it was necessary to identify the mechanisms by which binding of analyte to polymer occur, determine the optimal functional monomer to cross-linker ratio, and gain an understanding of the polymeric structure and characteristic bonds. An MIP was created using niacinamide (NAM) as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, azobisisobutyronitrile (AIBN) as the free-radical initiator, and chloroform as the porogen. It was hypothesized that rebinding occurs via hydrogen-bonding of the carbonyl and amide groups of NAM to the oxygen atoms in the carboxyl group of MAA. Rebinding studies were conducted using compounds with similar functional groups to NAM to determine binding mechanism to the polymer. Both the pyridyl nitrogen and the amide group were found to be important in hydrogen bonding interactions with the polymer. Polymers were optimized for rebinding by using different ratios of functional monomer:cross-linker (MAA:EGDMA) and determining imprint factor of NAM to each polymer. The 1:4 polymer yielded the highest imprinting factor, indicating that the polymer is most selective for NAM. FTIR was conducted to determine the structure of polymers created and whether NAM detection and quantification was possible. There was a peak at 1725 cm⁻¹, which was a shift of the C=O stretching band from 1694 cm⁻¹ in MAA and 1717 cm⁻¹ in EGDMA, indicating a chemical interaction between the two compounds. The disappearance of a peak at 1633 cm⁻¹ showed a loss of conjugation in the carboxylic acid in the polymeric structure. Through this research, knowledge was gained about the polymer optimization and structure. However, more studies need to be conducted to determine the feasibility of an MIP application for a lab-on-a-chip device.

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Mak, Wing Yin Winifred. "Developmental regulation of imprinted X inactivation". Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407945.

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Wang, Jinfang. "Xanthine-imprinted polymers for decaffeination applications". Thesis, University of Strathclyde, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431777.

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Abd, Bashar H. "Molecularly imprinted polymers for drug delivery". Thesis, University of Leicester, 2018. http://hdl.handle.net/2381/43042.

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Molecularly imprinted polymers (MIP) have received much attention and increased interest thanks to their excellent cost efficiency, robustness, high selectivity and simple short synthesis. The molecular imprinting process can be defined as creation of molecular recognition sites in a synthetic polymer. The template-derived sites thus created within the polymeric matrix allow MIP to selectively recognise and bind the target molecule. In light of these properties, MIP have been successfully applied in sensors, assays separation, and for drug delivery applications. Because of their small size, MIP nanoparticles (MIP NPs) can be used in biomedicine as specific drug delivery device, since the nanoscale format is potentially suitable for cellular or in vivo applications. This work has demonstrated that MIP NPs could be used as carriers for targeted drug delivery. For this purpose, the anti-inflammatory and anti-cancer agent curcumin was selected to develop a high throughput screening method which allows to optimise the controlled delivery of drugs using magnetic MIP NPs. Senescent cells which contribute to a number of pathophysiological conditions including fibrosis, diabetes, cancer, Alzheimer's and ageing, were selected as a model system to demonstrate the ability of specific MIP NPs to recognise them and deliver the cytotoxic drugs. Fluorescent MIP NPs specific for two epitopes of senescent cells B2M and DEP were prepared and characterised. In vitro tests based on two human cell lines have demonstrated the ability of the developed MIP NPs to recognise the senescent cells and confirmed that they were not toxic to the cells. In order to demonstrated the targeted drug delivery double-imprinted fluorescent MIP NPs with binding site specific for senescent cells and containing cytotoxic drugs Dasatnib and Gramicidin have been produced. In vitro tests with groups of old and young mice injected with MIP NPs demonstrated the targeted induction of cell death. It is possible to conclude that fluorescent MIP NPs could be effectively used as imaging tool for in vitro analysis as well as carriers for targeted delivery of the drugs in vivo. The protocols developed in this work are applicable for any other targets of clinical importance.

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Livros sobre o assunto "Imprinted"

Martín-Esteban, Antonio, ed. Molecularly Imprinted Polymers. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1629-1.

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Mattiasson, Bo, e Lei Ye, eds. Molecularly Imprinted Polymers in Biotechnology. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20729-2.

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1967-, Yan Mingdi, e Ramström Olof, eds. Molecularly imprinted materials: Science and technology. New York: Marcel Dekker, 2005.

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Bartsch, Richard A., e Mizuo Maeda, eds. Molecular and Ionic Recognition with Imprinted Polymers. Washington, DC: American Chemical Society, 1998. http://dx.doi.org/10.1021/bk-1998-0703.

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Kutner, Wlodzimierz, e Piyush Sindhu Sharma, eds. Molecularly Imprinted Polymers for Analytical Chemistry Applications. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010474.

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A, Bartsch Richard, Maeda Mizuo, American Chemical Society. Division of Industrial and Engineering Chemistry. e American Chemical Society Meeting, eds. Molecular and ionic recognition with imprinted polymers. Washington, DC: American Chemical Society, 1998.

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Liu, Zhaosheng, Yanping Huang e Yi Yang, eds. Molecularly Imprinted Polymers as Advanced Drug Delivery Systems. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0227-6.

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Lema, Aimée Zito. Imprinted mater: Aimée Zito Lema = Materia impresa : Aimée Zito Lema. Amsterdam: Looiersgracht 60, 2017.

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Karwoski, Christine Marrewa. Imprinted Identity: A History of Literature and Communal Selfhood in the Nath Sampradāy. [New York, N.Y.?]: [publisher not identified], 2020.

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Peter, Kofinas, Sellergren Börje, Roberts M. Joseph e Materials Research Society Meeting, eds. Molecularly imprinted materials--2003: Symposium held December 3-5, 2003, Boston, Massachusetts, U.S.A. Warrendale, Pa: Materials Research Society, 2004.

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Capítulos de livros sobre o assunto "Imprinted"

Hall, Andrew J., Marco Emgenbroich e Börje Sellergren. "Imprinted Polymers". In Topics in Current Chemistry, 317–49. Berlin, Heidelberg: Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/b104333.

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Whitcombe, Michael J., e Dhana Lakshmi. "Imprinted Polymers". In Electropolymerization, 133–51. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2010. http://dx.doi.org/10.1002/9783527630592.ch7.

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Carroll, Marilyn E., Peter A. Santi, Joseph Zohar, Thomas R. E. Barnes, Peter Verheart, Per Svenningsson, Per E. Andrén et al. "Imprinted Genes". In Encyclopedia of Psychopharmacology, 620. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1243.

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Ulubayram, Kezban. "Molecularly Imprinted Polymers". In Advances in Experimental Medicine and Biology, 123–38. Boston, MA: Springer US, 2004. http://dx.doi.org/10.1007/978-0-306-48584-8_10.

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Piletsky, Sergey A., Iva Chianella e Michael J. Whitcombe. "Molecularly Imprinted Polymers". In Encyclopedia of Biophysics, 1596–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-16712-6_719.

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Takeuchi, Toshifumi, e Hirobumi Sunayama. "Molecularly Imprinted Polymers". In Encyclopedia of Polymeric Nanomaterials, 1–5. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-36199-9_126-1.

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Haupt, Karsten, Ana V. Linares, Marc Bompart e Bernadette Tse Sum Bui. "Molecularly Imprinted Polymers". In Topics in Current Chemistry, 1–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/128_2011_307.

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Donato, Laura. "Imprinted Composite Membranes". In Encyclopedia of Membranes, 1027–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-44324-8_1612.

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Collinson, Maryanne M. "Imprinted Functionalized Silica". In The Supramolecular Chemistry of Organic-Inorganic Hybrid Materials, 581–98. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470552704.ch20.

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Kubo, Takuya, e Koji Otsuka. "Molecularly Imprinted Materials". In Handbook of Smart Materials in Analytical Chemistry, 159–78. Chichester, UK: John Wiley & Sons, Ltd, 2019. http://dx.doi.org/10.1002/9781119422587.ch5.

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Trabalhos de conferências sobre o assunto "Imprinted"

Haatainen, T., P. Majander, T. Makela e J. Ahopelto. "Imprinted 50 nm features by UV step and stamp imprint lithography method". In 2007 Digest of papers Microprocesses and Nanotechnology. IEEE, 2007. http://dx.doi.org/10.1109/imnc.2007.4456213.

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Zhenhe Chen, Bin Xue, Wentao Zhao, Linxia Zhang, Liquan Sun e Aiqin Luo. "High porosity lysozyme imprinted polymers". In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5964091.

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Spiclin, Ziga, Marko Bukovec, Franjo Pernus e Bostjan Likar. "Matching images of imprinted tablets". In 2007 IEEE Conference on Emerging Technologies & Factory Automation (EFTA 2007). IEEE, 2007. http://dx.doi.org/10.1109/efta.2007.4416881.

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Braga, Guilherme S., e Fernando J. Fonseca. "Molecularly imprinted sensor for isoborneol based on multilayered thin films of imprinted TiO2 nanoparticles". In 2017 ISOCS/IEEE International Symposium on Olfaction and Electronic Nose (ISOEN). IEEE, 2017. http://dx.doi.org/10.1109/isoen.2017.7968863.

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Anderson, John, Dmitry Pestov, Robert L. Fischer, Stanley Webb e Gary C. Tepper. "Fluorescence measurements of activity associated with a molecularly imprinted polymer imprinted to dipicolinic acid". In Optical Technologies for Industrial, Environmental, and Biological Sensing, editado por Arthur J. Sedlacek III, Richard Colton e Tuan Vo-Dinh. SPIE, 2004. http://dx.doi.org/10.1117/12.519480.

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Dauksher, W. J., N. V. Le, K. A. Gehoski, E. S. Ainley, K. J. Nordquist e N. Joshi. "An electrical defectivity characterization of wafers imprinted with step and flash imprint lithography". In Advanced Lithography, editado por Michael J. Lercel. SPIE, 2007. http://dx.doi.org/10.1117/12.712376.

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Tancharoen, Chompoonuch, Wannisa Sukjee, Chak Sangma e Thipvaree Wangchareansak. "Molecularly Imprinted Polymer for explosive detection". In 2015 Asian Conference on Defence Technology (ACDT). IEEE, 2015. http://dx.doi.org/10.1109/acdt.2015.7111605.

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Qi, Hang, Matthew Brown e David G. Lowe. "Low-Shot Learning with Imprinted Weights". In 2018 IEEE/CVF Conference on Computer Vision and Pattern Recognition (CVPR). IEEE, 2018. http://dx.doi.org/10.1109/cvpr.2018.00610.

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Chen, Zhenhe, Wentao Zhao, Aiqin Luo, Pablo Palomino e Eduardo Enciso. "Imprinted Photonic Crystals for Levodropropizine Sensing". In 2012 Symposium on Photonics and Optoelectronics (SOPO 2012). IEEE, 2012. http://dx.doi.org/10.1109/sopo.2012.6271015.

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Benito-Peña, Elena, Sergio Carrasco, Fernando Navarro-Villoslada, David R. Walt e María C. Moreno-Bondi. "Optically-based Molecularly Imprinted Polymers Sensors". In Optical Sensors. Washington, D.C.: OSA, 2017. http://dx.doi.org/10.1364/sensors.2017.setu2e.4.

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Relatórios de organizações sobre o assunto "Imprinted"

Xue, Ziling, Sheng Dai e Craig E. Barnes. Rational Synthesis of Imprinted Organofunctional Sol-Gel Materials for Toxic Metal Separation. Office of Scientific and Technical Information (OSTI), junho de 1999. http://dx.doi.org/10.2172/828521.

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XUE, Ziling, Craig E. Barnes e Sheng Dai. Rational Synthesis of Imprinted Organofunctional Sol-gel Materials for Toxic Metal Separation. Office of Scientific and Technical Information (OSTI), junho de 2000. http://dx.doi.org/10.2172/828522.

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Holthoff, Ellen L., Lily Li, Tobias Hiller e Kimberly L. Turner. A Molecularly Imprinted Polymer (MIP)-Coated Microbeam MEMS Sensor for Chemical Detection. Fort Belvoir, VA: Defense Technical Information Center, setembro de 2015. http://dx.doi.org/10.21236/ada622335.

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Jirtle, Randy L. Imprinted genes and transpositions: epigenomic targets for low dose radiation effects. Final report. Office of Scientific and Technical Information (OSTI), outubro de 2012. http://dx.doi.org/10.2172/1062638.

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Harvey, Scott D. Ultraselective Sorbents. Task 2: Molecularly Imprinted Polymers (MIPs)/Stabilized Antibody Fragments (STABs). Final Report FY 2004. Office of Scientific and Technical Information (OSTI), setembro de 2004. http://dx.doi.org/10.2172/15016482.

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Xue, Z., S. Dai e C. E. Barnes. Rational synthesis of imprinted organofunctional sol-gel materials for toxic metal separation. 1998 annual progress report. Office of Scientific and Technical Information (OSTI), junho de 1998. http://dx.doi.org/10.2172/13752.

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Harmon, Jerrel R. Use of a Fish Transportation Barge for Increasing Returns of Steelhead Imprinted for Homing, Final Report. Office of Scientific and Technical Information (OSTI), agosto de 1989. http://dx.doi.org/10.2172/5496291.

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Harmon, Jerrel R. Use of a Fish Transportation Barge for Increasing Returns of Steelhead Imprinted for Homing, 1985 Annual Report. Office of Scientific and Technical Information (OSTI), junho de 1986. http://dx.doi.org/10.2172/7146562.

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Holthoff, Ellen, e Dimitra Stratis-Cullum. A Nanosensor for Explosives Detection Based on Molecularly Imprinted Polymers (MIPs) and Surfaced-enhanced Raman Scattering (SERS). Fort Belvoir, VA: Defense Technical Information Center, março de 2010. http://dx.doi.org/10.21236/ada516676.

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Harmon, Jerrel R. Use of a Fish Transportation Barge for Increasing Returns of Steelhead Imprinted for Homing, 1986 Annual Report. Office of Scientific and Technical Information (OSTI), junho de 1987. http://dx.doi.org/10.2172/6280473.

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