Bibliografias temáticas / Isoenzymes Analysis

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Literatura científica selecionada sobre o tema "Isoenzymes Analysis"

Autor: Grafiati
Publicado: 4 de junho de 2021
Última modificação: 28 de janeiro de 2023

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Artigos de revistas sobre o assunto "Isoenzymes Analysis"

1

Tillyer, C. R., S. Rakhorst, and C. M. Colley. "Multicomponent analysis for alkaline phosphatase isoenzyme determination by multiple linear regression." Clinical Chemistry 40, no. 5 (May 1, 1994): 803–10. http://dx.doi.org/10.1093/clinchem/40.5.803.

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Abstract Alkaline phosphatase (EC 3.1.3.1) isoenzymes in serum may be determined by multicomponent analysis of the enzyme activities in the presence of multiple inhibitors. To determine inhibition coefficients of the isoenzymes, we used multiple linear regression analysis to compare alkaline phosphatase activities in the presence of known inhibitors with electrophoretically determined isoenzyme activities in plasma and serum samples. All possible combinations of exactly determined and overdetermined linear systems of inhibitors were ranked according to their prediction error to select an optim
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2

Dunaway, G. A., T. P. Kasten, T. Sebo, and R. Trapp. "Analysis of the phosphofructokinase subunits and isoenzymes in human tissues." Biochemical Journal 251, no. 3 (May 1, 1988): 677–83. http://dx.doi.org/10.1042/bj2510677.

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The 6-phosphofructo-1-kinase (PFK) subunits and isoenzymes were studied in human muscle, heart, brain, liver, platelets, fibroblasts, erythrocytes, placenta and umbilical cord. In each tissue, the subunit types in the native isoenzymes were characterized by immunological titration with subunit-specific antibodies and by column chromatography on QAE (quaternary aminoethyl)-Sephadex. Further, the subunits of the partially purified native isoenzymes were resolved by SDS/polyacrylamide-gel electrophoresis, identified by immunoblotting, and quantified by scanning gel densitometry of silver-stained
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3

Yoshikuni, Keiko, Takashi Matsuda, Janka Poracova, Akemi Sakai, Keiko Shimada, Noriko Tabuchi, and Kiyoh Tanishima. "Phylogenic Study of Denaturation of Lactate Dehydrogenase Isoenzymes from Different Species by High and Low Temperature." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 38, no. 5 (September 2001): 548–53. http://dx.doi.org/10.1177/000456320103800513.

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We studied the influence of storage at different temperatures on lactate dehydrogenase (EC 1.1.1.27; LD) isoenzymes from different tissues and different species, and analysed biochemical and biophysical mechanisms of denaturation during storage. Isoenzymes obtained from tissue extracts of mammals, poultry, reptiles, amphibians and fish were shown to have their own denaturation ranges at low temperatures by post-treatment assays and transition temperature analysis. These ranges were between − 10 and − 20°C for most vertebrate LD isoenzymes. Circular dichroism analysis indicated that the denatur
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Giannoulaki, E. E., D. L. Kalpaxis, C. Tentas, and P. Fessas. "Lactate dehydrogenase isoenzyme pattern in sera of patients with malignant diseases." Clinical Chemistry 35, no. 3 (March 1, 1989): 396–99. http://dx.doi.org/10.1093/clinchem/35.3.396.

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Abstract Total lactate dehydrogenase (LDH; EC 1.1.1.27) activity and the percentage distribution of LDH isoenzymes were determined in 127 patients with malignant diseases. A shift in the isoenzyme patterns was observed toward the M-type, with an increase in the percentage of LDH-4 and LDH-5 isoenzymes and a slight increase in total LDH activity of all patients. Serum samples from 68 of the patients contained an abnormal isoenzyme of LDH, "LDH-1 ex," that, on agarose gel electrophoresis at pH 8.6, migrated between albumin and LDH-1 isoenzyme. Chemotherapy, radiotherapy, or surgical removal of t
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Bar, Jair, Stuart Spencer, Shethah Morgan, Laura Pike, David Cunningham, Jane D. Robertson, Juliane M. Jürgensmeier, and Glenwood D. Goss. "Correlation of lactate dehydrogenase (LDH) isoenzyme profile with outcome in advanced colorectal cancer (CRC) patients (pts) treated with chemotherapy and bevacizumab (BEV) or cediranib (CED)." Journal of Clinical Oncology 30, no. 15_suppl (May 20, 2012): e13541-e13541. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.e13541.

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e13541 Background: There is a clinical need for predictive biomarkers of efficacy of VEGF signaling inhibitors (VSIs). LDH is a tetramer that can include any combination of the M and H subunits. LDH4 and 5 isoenzymes are composed mostly of the M subunit and are more abundant in hypoxic conditions. We speculated that the levels of LDH isoenzymes in pts serum may correlate with outcome of pts treated with VSIs. HORIZON I trial enrolled pts that were randomized to mFOLFOX6 with BEV or CED. A retrospective exploratory analysis was performed on baseline serum samples of these pts. Methods: Total se
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Pompeu, Georgia Bertoni, Priscila Lupino Gratão, Victor Alexandre Vitorello, and Ricardo Antunes Azevedo. "Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture." Scientia Agricola 65, no. 5 (2008): 548–52. http://dx.doi.org/10.1590/s0103-90162008000500015.

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Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analy
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7

Kochhar, Sunita, Christopher B. Watkins, Patricia L. Conklin, and Susan K. Brown. "A Quantitative and Qualitative Analysis of Antioxidant Enzymes in Relation to Susceptibility of Apples to Superficial Scald." Journal of the American Society for Horticultural Science 128, no. 6 (November 2003): 910–16. http://dx.doi.org/10.21273/jashs.128.6.0910.

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The activities and isoenzyme patterns of guaiacol-dependent peroxidase (POX), ascorbate peroxidase (APX), superoxide dismutase (SOD) and catalase (CAT) were studied in yellow- and red-fruited crab apple [Malus (L.) Mill.] selections from a `White Angel' × `Rome Beauty' cross that show differential susceptibility to the physiological storage disorder, superficial scald. There were no consistent relationships between total enzyme activities and scald incidence, high activities of the enzymes being detected in selections with both high and low susceptibilities to scald. However, additional indivi
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8

Singh, S. V., A. Kurosky та Y. C. Awasthi. "Human liver glutathione S-transferase ψ. Chemical characterization and secondary-structure comparison with other mammalian glutathione S-transferases". Biochemical Journal 243, № 1 (1 квітня 1987): 61–67. http://dx.doi.org/10.1042/bj2430061.

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The isolation and chemical characterization of the anionic human liver glutathione S-transferase (GST) psi (pI 5.5) are described and compared with other GST isoenzymes reported for rat and human. Amino acid compositional analysis, substrate specificity and isoelectric focusing indicated that GST psi is a unique isoenzyme form of GST. Strikingly, however, amino acid sequence analysis of the N-terminal region indicated that GST psi was identical with GST mu in the first 23 amino acid residues reported. It is likely that these two enzyme forms are at least partially structurally related. In orde
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9

Maekawa, M., K. Sudo, K. Iwahara, and T. Kanno. "Lactate dehydrogenase inhibition by immunoglobulin G in human serum." Clinical Chemistry 32, no. 7 (July 1, 1986): 1347–49. http://dx.doi.org/10.1093/clinchem/32.7.1347.

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Abstract Low lactate dehydrogenase (LD; EC 1.1.1.27) activity and an abnormal LD pattern in electrophoretograms of LD isoenzymes in the sera of two patients were caused by inhibition of LD by immunoglobulin G. One of these showed inhibitor activity in the serum upon direct analysis, while the other showed activity only after the immunoglobulin was stripped from the LD. As judged from the LD isoenzyme patterns in serum, the LD inhibitor appeared to act against M subunits. However, quantification of binding affinities to each isolated isoenzyme showed that the LD inhibitor had a stronger effect
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Segil, N., A. Shrutkowski, M. B. Dworkin, and E. Dworkin-Rastl. "Enolase isoenzymes in adult and developing Xenopus laevis and characterization of a cloned enolase sequence." Biochemical Journal 251, no. 1 (April 1, 1988): 31–39. http://dx.doi.org/10.1042/bj2510031.

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As part of a study of glycolysis during early development we have examined the pattern of expression of enolase isoenzymes in Xenopus laevis. In addition, the nucleotide sequence of a cDNA clone coding for the complete amino acid sequence of one enolase gene (ENO1) in X. laevis was determined. X. laevis ENO1 shows highest homology to mammalian non-neuronal enolase. Analysis of enolase isoenzymes in X. laevis by non-denaturing electrophoresis on cellulose acetate strips revealed five isoenzymes. One form was present in all tissues tested, two additional forms were expressed in oocytes, embryos,
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Teses / dissertações sobre o assunto "Isoenzymes Analysis"

1

Rena, Neil Graham. "Identification and analysis of phosphodiesterase isoenzymes." Thesis, University of Glasgow, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390767.

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MEJIA, DE LEON LUIS. "THE GENETICS OF ESTERASE ISOZYMES IN LETTUCE CULTIVARS." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183964.

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Lettuce, in its cultivated form (Lactuca sativa L.), is a crop of economic importance with several characteristics which make it well suited for biochemical and genetic studies. Biochemical traits such as proteins and enzymes have been studied extensively in many plant species and constitute an important experimental approach to physiological, evolutionary, taxonomic and breeding studies. Seed proteins were extracted from an array of lettuce cultivars. Polyacrylamide gel electrophoresis was employed in a comparative analysis of the soluble protein and isozyme characteristics of seed from each
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3

陳堅峰 and Kin-fung Chan. "Phylogenetic relationships and genetic diversity detected by rapd and isozyme analysis of crop and weedy species of amaranthus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1996. http://hub.hku.hk/bib/B29803846.

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Chan, Kin-fung. "Phylogenetic relationships and genetic diversity detected by rapd and isozyme analysis of crop and weedy species of amaranthus /." Hong Kong : University of Hong Kong, 1996. http://sunzi.lib.hku.hk/hkuto/record.jsp?B17665450.

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5

Ashari, Ir Sumeru. "Discrimination between citrus genotypes." Title page, contents and summary only, 1989. http://web4.library.adelaide.edu.au/theses/09A/09aa819.pdf.

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Granger, Andrew. "Genetic relationships and pollination studies in sweet cherry (Prunus avium L) /." Title page, table of contents and abstract only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phg758.pdf.

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7

King, Timothy L. (Timothy Lee). "Stock and Species Identification of Selected Marine Fishes and Shellfishes Using Allozyme Analysis and Isoelectric Focusing: Implications for Texas Fisheries Management." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc277919/.

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Allozyme frequencies and general protein patterns were surveyed among selected Texas marine fishes and shellfishes to illustrate the application of biochemical genetic techniques to stock and species identification in fisheries management.
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8

Mauricio, Isabel Larguinho. "Genetic diversity in the Leishmania donovani complex." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2000. http://researchonline.lshtm.ac.uk/682281/.

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The Leishmania donovani complex comprises four described species: L. donovani, L. archibaldi, L. infantum and L. chagasi. L. chagasi is the only New World species and has been considered similar to L. infantum, although some authors insist on maintenance of its independent species status. L. donovani has at least two major epidemiological subgroups whose relationships are poorly understood. In this thesis, molecular biological techniques were used to investigate the taxonomy and phylogenetic relationships within the L. donovani complex, with isoenzyme analysis (lEA) as reference technique. Ran
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9

Rowe, Daniel C. "Analysis of Toll-Like Receptor 4 Signal Transduction and IRF3 Activation in the Innate Immune Response: A Dissertation." eScholarship@UMMS, 2006. https://escholarship.umassmed.edu/gsbs_diss/163.

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Over the last decade, the innate immune system has been the subject of extensive research. Often overlooked by the robustness and specificity of the adaptive immune system, the innate immune system is proving to be just as complex. The identification of several families of pattern recognition receptors (PRRs) has revealed an ancient yet multifaceted system of proteins that are responsible for initiating host defense. A wide array of pathogens, from virus to bacteria, is detected using this assortment of receptors. One such family, the Toll-like receptors (TLRs), has been at the forefront of th
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10

Kwitshana, Zilungile L. "In vitro culture and isoenzyme analysis of giardia lamblia." Thesis, 1999. http://hdl.handle.net/10413/8226.

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Giardia lamblia, an enteric protozoan parasite, infects a large number of individuals worldwide. In South Africa prevalences ranging between 4 and 63% are documented, however, the impact of giardiasis is underreseached in this country. Giardia infections vary from asymptomatic carriage or a self-limiting acute symptomatic illness to chronic, debilitating malabsorption syndrome. The factors responsible for development of symptomatic versus asymptomatic infection are poorly understood. It is believed by some that host factors determine the clinical outcome of infection. On the other hand, the po
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Livros sobre o assunto "Isoenzymes Analysis"

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Izmenchivostʹ izofermentnykh spektrov peroksidazy u sosny obyknovennoĭ. Sverdlovsk: Akademii͡a︡ nauk SSSR, Uralʹskiĭ nauch. t͡s︡entr, 1985.

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2

Interpretation of protein and isoenzyme patterns in body fluids. New York: Igaku-Shoin, 1991.

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3

Menendez, Ricardo A. Identification of apple (Malus domestica Borkh.) clones based on isozymic diversity. 1985.

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4

Practical isozyme genetics. Chichester: Ellis Horwood, 1988.

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5

Biochemical and immunological analyses of creatine kinase isoenzymes from human heart and skeletal muscle. 1988.

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6

Biochemical and immunological analyses of creatine kinase isoenzymes from human heart and skeletal muscle. 1988.

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7

Biochemical and immunological analyses of creatine kinase isoenzymes from human heart and skeletal muscle. 1988.

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8

Biochemical and immunological analyses of creatine kinase isoenzymes from human heart and skeletal muscle. 1986.

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9

Serum creatine kinase (CK) and CK-MB isoenzyme responses to acute and prolonged swimming in trained athletes. 1985.

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Capítulos de livros sobre o assunto "Isoenzymes Analysis"

1

Millar, Thomas J., and Harry Koutavas. "Analysis of Phosphodiesterase Isoenzymes in the Ocular Glands of the Rabbit." In Lacrimal Gland, Tear Film, and Dry Eye Syndromes 2, 153–56. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5359-5_21.

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2

Katoch, Rajan. "Isoenzyme Analysis." In Analytical Techniques in Biochemistry and Molecular Biology, 227–31. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-9785-2_11.

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3

Kuan, Shia S., and George G. Guilbault. "Immunochemical Determination of Creatine Kinase Isoenzyme-MB." In Electrochemical Sensors in Immunological Analysis, 145–65. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4899-1974-8_11.

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4

Dardé, M. L., B. Bouteille, and M. Pestre-Alexandre. "Study of Genetic Polymorphism of Toxoplasma Gondii Through Isoenzyme Analysis." In Toxoplasmosis, 9–17. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-78559-7_2.

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Sen, Asok K. "Control Analysis of Isoenzymic Pathways: Application of Directed Graphs and Electrical Analogs." In Modern Trends in Biothermokinetics, 237–42. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2962-0_38.

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Gramiccia, M., L. Gradoni, and M. C. Angelici. "Epidemiology of Mediterranean Leishmaniasis Caused by Leishmania Infantum: Isoenzyme and kDna Analysis for The Identification of Parasites from Man, Vectors and Reservoirs." In Leishmaniasis, 21–37. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-1575-9_3.

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Dumas-Gaudot, E., B. Dassi, S. Slezack, V. Gianinazzi-Pearson, and S. Gianinazzi. "Targeted Approaches for Detecting Changes in Protein Expression with Mycorrhiza Development: Hydrolytic Isoenzyme Analyses and Immunological Detection of Known Proteins in Root Extracts." In Mycorrhiza Manual, 289–309. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-60268-9_19.

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Gardner, Natwaine Sherune, Kedon J. S. Luke, Andrew O. Wheatley, Winston De La Haye, Perceval Steven Bahado-Singh, Lowell L. Dilworth, Donovan A. McGrowder, et al. "Plasma Cocaine Metabolite Levels and Liver CYP450 3A4 Isoenzyme Activity as Indicators of Cocaine Metabolism in Rats Treated With Salako Supplements." In Strategic Applications of Measurement Technologies and Instrumentation, 1–21. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-5406-6.ch001.

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The effects of Salako nutritional supplements on cocaine-dependent Sprague Dawley rats was investigated. Rats were made cocaine-dependent using conditioned place preference (CPP) where craving was analyzed regularly. Cocaine metabolite levels were determined from blood samples. CYP450 3A4 isoenzyme activities were obtained using liver homogenate. Statistical analysis was done using SPSS one-way ANOVA and Duncans multiple range test. Results show that when cocaine use was discontinued, the supplements reduced craving of cocaine significantly. Blood plasma results showed higher benzoylecgonine equilibrium possibly indicating that the supplements aided the removal of stored cocaine metabolites which may have contributed to better management of craving in the rats. CYP450 3A4 isoenzyme activity was further enhanced by the supplements and is indicative of increased cocaine metabolism. The results indicate that the Salako nutritional supplements reduce craving caused by chronic cocaine administration by increasing the liver CYP450 3A4 isoenzyme activity, resulting in better plasma clearance.
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K., Malgorzata. "Isoenzyme Analyses Tools Used Long Time in Forest Science." In Electrophoresis. InTech, 2012. http://dx.doi.org/10.5772/45756.

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Tenor, Hermann, and Christian Schudt. "Analysis of PDE Isoenzyme Profiles in Cells and Tissues by Pharmacological Methods." In Phosphodiesterase Inhibitors, 21–40. Elsevier, 1996. http://dx.doi.org/10.1016/b978-012210720-7/50004-6.

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Trabalhos de conferências sobre o assunto "Isoenzymes Analysis"

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Staykova, Teodora, Panomir Tzenov, Yolanda Vasileva, Dimitar Grekov, and Krasimira Avramova. "POPULATION GENETIC ANALYSIS OF SILKWORM BREEDS BASED ON ISOENZYME MARKERS." In XIth International Congress of Geneticists and Breeders from the Republic of Moldova. Scientific Association of Geneticists and Breeders of the Republic of Moldova, Institute of Genetics, Physiology and Plant Protection, Moldova State University, 2021. http://dx.doi.org/10.53040/cga11.2021.111.

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Relatórios de organizações sobre o assunto "Isoenzymes Analysis"

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Watkins, Chris B., Susan Lurie, Amnon Lers, and Patricia L. Conklin. Involvement of Antioxidant Enzymes and Genes in the Resistance Mechanism to Postharvest Superficial Scald Development. United States Department of Agriculture, December 2004. http://dx.doi.org/10.32747/2004.7586539.bard.

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The objective of this research project was to evaluate the involvement of antioxidant enzymes and genes in the resistance mechanism to postharvest superficial scald development using two primary systems: 1. Resistant and susceptible progenies of an apple cross between a scald resistant crab apple, ‘White Angel’ and a scald susceptible cultivar, ‘Rome Beauty’; 2. Heat-treatment of ‘Granny Smith’, which is known to reduce scald development in this cultivar. In 2002 we asked for, and received (October 14), permission to revise our initial objectives. The US side decided to expand their results to
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