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Artigos de revistas sobre o tema "Laboratory reagent surveillance"

Autor: Grafiati

Publicado: 5 de abril de 2025

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1

Gachogo, Rachael W., Daniel N. Mwai, and Frank G. Onyambu. "Cost analysis of implementing HIV drug resistance testing in Kenya: a case study of a service delivery site at a tertiary level hospital in Kenya." F1000Research 9 (July 29, 2020): 793. http://dx.doi.org/10.12688/f1000research.23379.1.

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Background: HIV drug resistance (HIVDR) threatens progress achieved in response to the HIV epidemic. Understanding the costs of implementing HIVDR testing programs for patient management and surveillance in resource-limited settings is critical in optimizing resource allocation. Here, we estimate the unit cost of HIVDR testing and identify major cost drivers while documenting challenges and lessons learnt in implementation of HIVDR testing at a tertiary level hospital in Kenya. Methods: We employed a mixed costing approach to estimate the costs associated with performing a HIVDR test from the

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Solsvik, Anne Elisabeth, Ann Helen Kristoffersen, Sverre Sandberg, et al. "A national surveillance program for evaluating new reagent lots in medical laboratories." Clinical Chemistry and Laboratory Medicine (CCLM) 60, no. 3 (2022): 351–60. http://dx.doi.org/10.1515/cclm-2021-1262.

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Abstract Objectives Differences between laboratory results attributable to the use of different reagent lots can potentially affect the diagnosis and monitoring of patients. To minimize patient risks, all laboratories should verify that new reagent lots meet agreed analytical performance specifications (APS). We propose a simplified, pragmatic approach for laboratories that involves compilating results into a national surveillance program, and present the first results obtained when applying this approach to troponins, glycated hemoglobin (HbA1c), prostate-specific antigen (PSA) and D-dimer. M

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Prokhvatilova, E. V., N. N. Teteryatnikova, I. B. Zakharova, L. I. Belitskaya, D. V. Viktorov, and A. V. Toporkov. "PREPARATION FOR STATE REGISTRATION OF THE REAGENT KIT FOR THE DETECTION AND DIFFERENTIATION OF THE DNA OF BURKHOLDERIA «PSEUDOMALLEI» GROUP." Russian Clinical Laboratory Diagnostics 64, no. 3 (2019): 180–85. http://dx.doi.org/10.18821/0869-2084-2019-64-3-180-185.

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The reagent kit designed to detect and simultaneously differentiate the DNA of three species of Burkholderia pseudomallei - causative agents of melioidosis (B. pseudomallei), glanders (B. mallei) and B. thailandensis by the set of genes of β-lactamases with B and D molecular classes using a multiplex polymerase chain reaction with electrophoretic detection was developed for clinical laboratory diagnosis. The functional properties of the reagent kit were evaluated, tests were carried out, the stages of examination and registration in the Federal Service for Surveillance on Consumer Rights’ Prot

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Castilho-Pelloso, Marcela Peres, Dina Lúcia Morais Falavigna, and Ana Lúcia Falavigna-Guilherme. "Suspected acute toxoplasmosis in pregnant women." Revista de Saúde Pública 41, no. 1 (2007): 27–34. http://dx.doi.org/10.1590/s0034-89102007000100005.

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OBJECTIVE: To determine the prevalence of reagent serology for suspected acute toxoplasmosis in pregnant women and to describe clinical, laboratory and therapeutic profiles of mothers and their children. METHODS: A retrospective study was conducted with IgM-anti-Toxoplasma gondii-reagent pregnant women and their children who attended the public health system in the state of Paraná, Southern Brazil, from January 2001 to December 2003. Information were obtained from clinical, laboratory (ELISA IgM/IgG) and ultrasonographic data and from interviews with the mothers. To test the homogeneity of the

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Sitko, John C., James Jordan Steel, Erin A. Almand, et al. "Efficiency of pooled surveillance testing in academic labs to detect and inhibit COVID-19 outbreaks." Bioanalysis 13, no. 15 (2021): 1177–82. http://dx.doi.org/10.4155/bio-2021-0054.

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Robust surveillance testing is a key strategic plan to prevent COVID-19 outbreaks and slow the spread of the SARS-CoV-2 pandemic; however, limited resources, facilities and time often impair the implementation of a widespread surveillance effort. To mitigate these resource limitations, we employed a strategy of pooling samples, reducing reagent cost and processing time. Through utilizing academic faculty and labs, successful pooled surveillance testing was conducted throughout Fall 2020 semester to detect positive SARS-CoV-2 infections in a population of 4400 students. During the semester, ove

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Hapsari, Rebriarina, Irfan Kesumayadi, Nani Maharani, Endang Mahati, Ferdy Kurniawan Cayami, and Sutopo Patria Jati. "The efficiency of selective pooling strategy in a COVID-19 diagnostic laboratory." Journal of Infection in Developing Countries 16, no. 08 (2022): 1278–84. http://dx.doi.org/10.3855/jidc.14359.

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Introduction: Mass testing is essential in the surveillance strategy for fighting the COVID-19 pandemic. It allows early detection of suspected cases and subsequently early isolation to mitigate spread. However, the high cost and limited consumables and reagents hinder the mass testing strategy in developing countries such as Indonesia. The specimen pooling strategy is an option to perform mass screening with limited resources. This study aims to determine the positivity rate cut-off and to evaluate the efficiency of pooling strategy for the laboratory diagnosis of COVID-19. Methodology:

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Gordon, Ilyssa O., Jodi D. Sherman, Michael Leapman, Michael Overcash, and Cassandra L. Thiel. "Life Cycle Greenhouse Gas Emissions of Gastrointestinal Biopsies in a Surgical Pathology Laboratory." American Journal of Clinical Pathology 156, no. 4 (2021): 540–49. http://dx.doi.org/10.1093/ajcp/aqab021.

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Abstract Objectives Given adverse health effects of climate change and contributions of the US health care sector to greenhouse gas (GHG) emissions, environmentally sustainable delivery of care is needed. We applied life cycle assessment to quantify GHGs associated with processing a gastrointestinal biopsy in order to identify emissions hotspots and guide mitigation strategies. Methods The biopsy process at a large academic pathology laboratory was grouped into steps. Each supply and reagent was catalogued and postuse treatment noted. Energy consumption was estimated for capital equipment. Two

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Mosa, Alexander I. "CRISPR-Based Diagnostics for Point-of-Care Viral Detection." International Journal of Translational Medicine 2, no. 2 (2022): 198–203. http://dx.doi.org/10.3390/ijtm2020017.

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Point-of-care detection of viral infection is required for effective contact-tracing, epidemiological surveillance, and linkage to care. Traditional diagnostic platforms relying on either antigen detection or nucleic amplification are limited by sensitivity and the need for costly laboratory infrastructure, respectively. Recently, CRISPR-based diagnostics have emerged as an alternative, combining equipment light workflows with high specificity and sensitivity. However, as a nascent technology, several outstanding challenges to widespread field deployment remain. These include the need for pre-

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Nov, Vandarith, Darapheak Chau, Kimsorn Pa, Keodane Hem, and Sidonn Krang. "Proficiency Testing Performances Analysis of Microbiology Laboratories Participating in Cambodia Antimicrobial Resistance (AMR) Surveillance System." Infection Control & Hospital Epidemiology 41, S1 (2020): s360—s361. http://dx.doi.org/10.1017/ice.2020.984.

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Background: The WHO recommends the establishment of sustainable and evidence-based surveillance systems are recommended for the prevention of microbial resistance. For these surveillance systems, all medical microbiology laboratories are required to participate an external quality assessment (EQA) program that covers antimicrobial susceptibility testing (AST). Clinical microbiology EQA panels with 3 isolates have been provided 3 times per year to antimicrobial resistance (AMR) sentinel laboratories in Cambodia since 2012. We evaluated the performance of laboratory testing implemented between 2

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Votintseva, Antonina A., Phelim Bradley, Louise Pankhurst, et al. "Same-Day Diagnostic and Surveillance Data for Tuberculosis via Whole-Genome Sequencing of Direct Respiratory Samples." Journal of Clinical Microbiology 55, no. 5 (2017): 1285–98. http://dx.doi.org/10.1128/jcm.02483-16.

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ABSTRACT Routine full characterization of Mycobacterium tuberculosis is culture based, taking many weeks. Whole-genome sequencing (WGS) can generate antibiotic susceptibility profiles to inform treatment, augmented with strain information for global surveillance; such data could be transformative if provided at or near the point of care. We demonstrate a low-cost method of DNA extraction directly from patient samples for M. tuberculosis WGS. We initially evaluated the method by using the Illumina MiSeq sequencer (40 smear-positive respiratory samples obtained after routine clinical testing and

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Fry, Norman K., John Duncan, Karen Wagner, et al. "Role of PCR in the diagnosis of pertussis infection in infants: 5 years' experience of provision of a same-day real-time PCR service in England and Wales from 2002 to 2007." Journal of Medical Microbiology 58, no. 8 (2009): 1023–29. http://dx.doi.org/10.1099/jmm.0.009878-0.

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As part of an enhanced surveillance programme for pertussis in England and Wales, a real-time PCR service for the detection of Bordetella pertussis was introduced for infants aged ≤6 months admitted to a paediatric intensive care unit or paediatric ward with a respiratory illness compatible with pertussis. Two real-time fluorescent resonance energy transfer hybridization probe LightCycler (Roche Diagnostics) PCR assays were used. One (designed in-house) targeted the pertussis toxin S1 promoter (ptxA-pr), and included an internal process control to test for sample inhibition and reagent perform

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Garcia, Gabriela A., Anton R. Lord, Lilha M. B. Santos, et al. "Rapid and Non-Invasive Detection of Aedes aegypti Co-Infected with Zika and Dengue Viruses Using Near Infrared Spectroscopy." Viruses 15, no. 1 (2022): 11. http://dx.doi.org/10.3390/v15010011.

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The transmission of dengue (DENV) and Zika (ZIKV) has been continuously increasing worldwide. An efficient arbovirus surveillance system is critical to designing early-warning systems to increase preparedness of future outbreaks in endemic countries. The Near Infrared Spectroscopy (NIRS) is a promising high throughput technique to detect arbovirus infection in Ae. aegypti with remarkable advantages such as cost and time effectiveness, reagent-free, and non-invasive nature over existing molecular tools for similar purposes, enabling timely decision making through rapid detection of potential di

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Shastri, Aditi, Kelty R. Baker, and Perumal Thiagarajan. "Differential Effect of An Autoantibody to Thrombin on Fibrinogen Cleavage and Protein C Activation." Blood 116, no. 21 (2010): 3652. http://dx.doi.org/10.1182/blood.v116.21.3652.3652.

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Abstract Abstract 3652 A 76 year old male experienced an unexpected blood loss of 1300 ml during an elective left shoulder replacement for degenerative joint disease. His post-operative course was uncomplicated. Further laboratory testing revealed a prolonged thrombin time of 33 seconds (control of 17.7–20.3 seconds). The reptilase time was within normal limits. He also had a normal factor V, × and fibrinogen level. The prolonged thrombin time failed to correct upon addition of normal plasma. The patient's IgG fraction was isolated by protein G-Sepharose chromatography and tested for its effec

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Siahaan, Selma, Rukmini Rukmini, Betty Roosihermiatie, et al. "The Effort to Rationalize Antibiotic Use in Indonesian Hospitals: Practice and Its Implication." Journal of Tropical Medicine 2023 (February 25, 2023): 1–12. http://dx.doi.org/10.1155/2023/7701712.

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An effective strategy for combatting AMR in Indonesia is to make the use of antibiotics in hospitals more rational with the help of an Antimicrobial Resistance Control Program (AMR-CP). This study aims to analyze the implementation of the AMR-CP in hospitals by conducting in-depth interviews with health professionals from ten hospitals and health officers of ten provincial health offices in ten different provinces and observation towards its documents. The sample location was selected by purposive sampling. Informants at the hospitals were hospital directors, chairmen of the AMR-CP team, chair

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Kay A., Bradford. "The role of the laboratory in disease surveillance." Eastern Mediterranean Health Journal 2, no. 1 (2021): 68–72. http://dx.doi.org/10.26719/1996.2.1.68.

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Laboratory information is critical for disease surveillance and control programmes. Before an outbreak, laboratory-supported surveillance allows early detection of cases. During an outbreak a sample of cases should be laboratory confirmed to assess changes in the etiological agent and to guide decisions about the allocation of resources. Support is provided by laboratories of differing capabilities. Field laboratories are useful in areas where resources are limited or nonexistent. More complete testing is usually done in regional laboratories. International reference laboratories may identify

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Tsutsumi, Tamao, Charles Frenette, Nancy Doherty, Jacqueline Sedman, and Ashraf Ismail. "243. Transflection Fourier Transform Infrared Spectroscopy as a Real-Time Strain Typing Technique: A Vancomycin-Resistant Enterococcus faecium (VRE) Typing Prospective Study." Open Forum Infectious Diseases 6, Supplement_2 (2019): S138. http://dx.doi.org/10.1093/ofid/ofz360.318.

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Abstract Background Rapid bacterial strain typing for nosocomial outbreak surveillance is critical for timely outbreak detection and implementation of appropriate infection control protocols in hospitals. Pulsed-field gel electrophoresis (PFGE) remains the gold standard for strain typing, but it has the disadvantages of being time-consuming and costly. Transflection Fourier transform infrared (FTIR) spectroscopy is a nondestructive and reagent-free technique for rapid microbial identification and subspecies-level discrimination. The potential of employing transflection FTIR spectroscopy as a r

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Roberts, Tamalee, Nantasit Luangasanatip, Clare L. Ling, et al. "Antimicrobial resistance detection in Southeast Asian hospitals is critically important from both patient and societal perspectives, but what is its cost?" PLOS Global Public Health 1, no. 10 (2021): e0000018. http://dx.doi.org/10.1371/journal.pgph.0000018.

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Antimicrobial resistance (AMR) is a major threat to global health. Improving laboratory capacity for AMR detection is critically important for patient health outcomes and population level surveillance. We aimed to estimate the financial cost of setting up and running a microbiology laboratory for organism identification and antimicrobial susceptibility testing as part of an AMR surveillance programme. Financial costs for setting up and running a microbiology laboratory were estimated using a top-down approach based on resource and cost data obtained from three clinical laboratories in the Mahi

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Aboushady, Ahmed Taha, Olivier Manigart, Abdourahmane Sow, et al. "Surveillance of Antimicrobial Resistance in the ECOWAS Region: Setting the Scene for Critical Interventions Needed." Antibiotics 13, no. 7 (2024): 627. http://dx.doi.org/10.3390/antibiotics13070627.

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Antimicrobial resistance poses a significant challenge to public health globally, leading to increased morbidity and mortality. AMR surveillance involves the systematic collection, analysis, and interpretation of data on the occurrence and distribution of AMR in humans, animals, and the environment for action. The West African Health Organization, part of the Economic Community of West African States (ECOWAS), is committed to addressing AMR in the region. This paper examines the status of AMR surveillance in ECOWAS countries using available WHO data from the TrACSS survey and GLASS enrollments

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Bingen, E. H., E. Denamur, and J. Elion. "Use of ribotyping in epidemiological surveillance of nosocomial outbreaks." Clinical Microbiology Reviews 7, no. 3 (1994): 311–27. http://dx.doi.org/10.1128/cmr.7.3.311.

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Over the past few years, genotypic methods based on the study of bacterial DNA polymorphism have shown high discriminatory power for strain differentiation and superiority over most phenotypic methods commonly available in the clinical microbiology laboratory. Some of the methods used, however, required either a high level of technology and sophisticated equipment (e.g., pulsed-field gel electrophoresis) or species-specific reagents of restricted availability (randomly cloned DNA probes or gene-specific probes). Because ribotyping uses a universal probe (rRNA) and is a rather simple technology

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Moreno-Contreras, Joaquín, Marco A. Espinoza, Carlos Sandoval-Jaime, et al. "Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings." PLOS ONE 17, no. 1 (2022): e0263114. http://dx.doi.org/10.1371/journal.pone.0263114.

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In many countries a second wave of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has occurred, triggering a shortage of reagents needed for diagnosis and compromising the capacity of laboratory testing. There is an urgent need to develop methods to accelerate the diagnostic procedures. Pooling samples represents a strategy to overcome the shortage of reagents, since several samples can be tested using one reaction, significantly increasing the number and speed with which tests can be carried out. We have reported the feasibility to use a direct lysis pro

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Balmaseda, Ángel, Saira Saborío Galo, Karla González, et al. "Development of in-house serological methods for diagnosis and surveillance of chikungunya." Revista Panamericana de Salud Pública 41 (June 29, 2017): 1. http://dx.doi.org/10.26633/rpsp.2017.56.

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Objective.To develop and evaluate serological methods for chikungunya diagnosis and research in Nicaragua.Methods.Two IgM ELISA capture systems (MAC-ELISA) for diagnosis of acute chikungunya virus (CHIKV) infections, and two Inhibition ELISA Methods (IEM) to measure total antibodies against CHIKV were developed using monoclonal antibodies (mAbs) and hyperimmune serum at the National Virology Laboratory of Nicaragua in 2014–2015. The sensitivity, specificity, predictive values, and agreement of the MAC-ELISAs were obtained by comparing the results of 198 samples (116 positive; 82 negative) with

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Panwalker, Anand P., and Elizabeth Fuhse. "Nosocomial Mycobacterium gordonae Pseudoinfection From Contaminated Ice Machines." Infection Control 7, no. 2 (1986): 67–70. http://dx.doi.org/10.1017/s0195941700063918.

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AbstractThirty-two clinical specimens submitted to the laboratory during a 12-month period from July 1980 to June 1981 were reported to be culture-positive for Mycobacterium gordonae, an organism generally considered to be a slow-growing saprophyte with natural habitats which include soil and water. Only seven similar isolates had been recovered in the preceding 4½ year period. The discordance between clinical findings and the mycobacterial cultures suggested extrinsic contamination of the specimens. Contamination in the laboratory was believed unlikely because: 1) clinical samples obtained in

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Chidzaye, Robert W. "Assessing Barriers to Medical Laboratory Diagnostic Service Delivery in Mzuzu City." International Journal of Biomedical Science 15, no. 1 (2019): 32–56. http://dx.doi.org/10.59566/ijbs.2019.15032.

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Medical laboratories provide confirmatory diagnosis and evidence based management of diseases, essential public health information and disease surveillance. A wide variety of research studies suggest that breakdowns in the diagnostic process result in a staggering toll of harm, patient deaths and wastage of valuable medical resources already constrained in the developing world. The objective of this study was to assess barriers to delivery of optimal laboratory diagnostic services in Mzuzu city. This was a descriptive cross-sectional study using quantitative research approach. Three categories

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Dara, Antoine, Bourema Kouriba, Amadou Daou, et al. "Sequencing SARS-CoV-2 in a Malaria Research Laboratory in Mali, West Africa: The Road to Sequencing the First SARS-CoV-2 Genome in Mali." Processes 9, no. 12 (2021): 2169. http://dx.doi.org/10.3390/pr9122169.

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Next-generation sequencing (NGS) has become a necessary tool for genomic epidemiology. Even though the utility of genomics in human health has been proved, genomic surveillance has never been as important as during the COVID-19 pandemic. This has been demonstrated by the recent use of genomic surveillance to detect new variants of SARS-CoV-2 in the United Kingdom, South Africa, and Brazil. Until recently, Malian scientists did not have access to any local NGS platform, and samples had to be shipped abroad for sequencing. Here, we report on how we adapted a laboratory setup for Plasmodium resea

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Dalpke, Alexander H., Marjeta Hofko, and Stefan Zimmermann. "Development of a Real-Time PCR Protocol Requiring Minimal Handling for Detection of Vancomycin-Resistant Enterococci with the Fully Automated BD Max System." Journal of Clinical Microbiology 54, no. 9 (2016): 2321–29. http://dx.doi.org/10.1128/jcm.00768-16.

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Vancomycin-resistant enterococci (VRE) are an important cause of health care-associated infections, resulting in significant mortality and a significant economic burden in hospitals. Active surveillance for at-risk populations contributes to the prevention of infections with VRE. The availability of a combination of automation and molecular detection procedures for rapid screening would be beneficial. Here, we report on the development of a laboratory-developed PCR for detection of VRE which runs on the fully automated Becton Dickinson (BD) Max platform, which combines DNA extraction, PCR setu

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Alkholidy, Ghamdan Gamal, Labiba Saeed Anam, Ali Hamoud Almahaqri, and Yousef Khader. "Performance of the Severe Acute Respiratory Illness Sentinel Surveillance System in Yemen: Mixed Methods Evaluation Study." JMIR Public Health and Surveillance 7, no. 7 (2021): e27621. http://dx.doi.org/10.2196/27621.

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Background The national severe acute respiratory illness (SARI) surveillance system in Yemen was established in 2010 to monitor SARI occurrence in humans and provide a foundation for detecting SARI outbreaks. Objective To ensure that the objectives of national surveillance are being met, this study aimed to examine the level of usefulness and the performance of the SARI surveillance system in Yemen. Methods The updated Centers for Disease Control and Prevention guidelines were used for the purposes of our evaluation. Related documents and reports were reviewed. Data were collected from 4 centr

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Brangel, Polina, Sina Tureli, Barbara Mühlemann, et al. "A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories." Viruses 16, no. 12 (2024): 1936. https://doi.org/10.3390/v16121936.

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Setting up a global SARS-CoV-2 surveillance system requires an understanding of how virus isolation and propagation practices, use of animal or human sera, and different neutralisation assay platforms influence assessment of SARS-CoV-2 antigenicity. In this study, with the contribution of 15 independent laboratories across all WHO regions, we carried out a controlled analysis of neutralisation assay platforms using the first WHO International Standard for antibodies to SARS-CoV-2 variants of concern (source: NIBSC). Live virus isolates (source: WHO BioHub or individual labs) or spike plasmids

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Bankamp, Bettina, Raydel Anderson, Lijuan Hao, et al. "Building Quality Control for Molecular Assays in the Global Measles and Rubella Laboratory Network." Vaccines 12, no. 8 (2024): 824. http://dx.doi.org/10.3390/vaccines12080824.

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More than 100 laboratories in the World Health Organization Global Measles and Rubella Laboratory Network (GMRLN) perform nucleic acid-based methods for case confirmation of measles or rubella infections and/or strain surveillance (genotyping). The quality of laboratory data is critical to ensure that diagnostic results and country reports to regional verification committees are based on accurate data. A molecular External Quality Assurance (mEQA) program was initiated by the US-CDC in 2014 to evaluate the performance of laboratories in the network. The inclusion of testing for measles and rub

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Warrington, Jill S., Jessica W. Crothers, Andrew Goodwin, et al. "All Hands-On Deck and All Decks on Hand: Surmounting Supply Chain Limitations During the COVID-19 Pandemic." Academic Pathology 8 (January 1, 2021): 237428952110119. http://dx.doi.org/10.1177/23742895211011928.

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Testing during the COVID-19 pandemic has been crucial to public health surveillance and clinical care. Supply chain constraints—spanning limitations in testing kits, reagents, pipet tips, and swabs availability—have challenged the ability to scale COVID-19 testing. During the early months, sample collection kits shortages constrained planned testing expansions. In response, the University of Vermont Medical Center, University of Vermont College of Medicine, Vermont Department of Health Laboratory, Aspenti Health, and providers across Vermont including 16 area hospitals partnered to surmount th

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Buchta, Christoph, Jeremy V. Camp, Jovana Jovanovic, et al. "The versatility of external quality assessment for the surveillance of laboratory and in vitro diagnostic performance: SARS-CoV-2 viral genome detection in Austria." Clinical Chemistry and Laboratory Medicine (CCLM) 59, no. 10 (2021): 1735–44. http://dx.doi.org/10.1515/cclm-2021-0604.

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Abstract Objectives External quality assessment (EQA) schemes provide information on individual and general analytical performance of participating laboratories and test systems. The aim of this study was to investigate the use and performance of SARS-CoV-2 virus genome detection systems in Austrian laboratories and their preparedness to face challenges associated with the pandemic. Methods Seven samples were selected to evaluate performance and estimate variability of reported results. Notably, a dilution series was included in the panel as a measure of reproducibility and sensitivity. Severa

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Aryastami, Ketut, Harimat Hendarwan, Vivi Setiawaty, et al. "Laboratory preparedness to support the Covid-19 pandemic respond in Indonesia." Health Science Journal of Indonesia 11, no. 2 (2020): 138–46. http://dx.doi.org/10.22435/hsji.v11i2.4089.

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Latar belakang: Penyakit jenis baru COVID-19 yang disebabkan oleh virus corona menjadi sebuah pandemic di akhir tahun 2019. Kota Wuhan (China) merupakan lokasi pertama terdeteksinya kasus COVID-19. Tanpa adanya kecurigaan apapun penyakit ini dengan cepatnya menyebar ke seluruh dunia mengikuti alur mobilitas manusia. Dalam kondisi tersebut sistem kesehatan di setiap negara tampak kelabakan khususnya dalam pengendalian transmisi penyakit. Studi ini ingin mengidentifikasi kesiapan jejaring laboratorium kesehatan di Indonesia.  Metode: Penilaian cepat dilakukan terhadap ketersediaan dan kesi

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Polonis, Katarzyna, Joseph H. Blommel, Andrew E. O. Hughes, David Spencer, Joseph A. Thompson, and Molly C. Schroeder. "Innovations in Short-Read Sequencing Technologies and Their Applications to Clinical Genomics." Clinical Chemistry 71, no. 1 (2025): 97–108. https://doi.org/10.1093/clinchem/hvae173.

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Abstract Background Massively parallel sequencing (MPS) of nucleic acids has been a transformative technology for basic and applied genomic science, increasing efficiencies and decreasing costs to enable studies of unprecedented scope and impact. In clinical settings, these technological and scientific advances have led to the development of tests that are increasingly fast, comprehensive, and more frequently employed. Practitioners of genomic medicine have applied these tools across clinical settings, including diagnosis of inherited disorders and cancers and infectious disease detection and

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Esman, Anna, Anna Cherkashina, Konstantin Mironov, et al. "SARS-CoV-2 Variants Monitoring Using Real-Time PCR." Diagnostics 12, no. 10 (2022): 2388. http://dx.doi.org/10.3390/diagnostics12102388.

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According to the temporary recommendations of the 2021 World Health Organization (WHO), in addition to whole-genome sequencing, laboratories in various countries can also screen for known mutations utilizing targeted RT-PCR-based mutation detection assays. The aim of this work was to generate a laboratory technique to differentiate the main circulating SARS-CoV-2 variants in 2021–2022, when a sharp increase in morbidity was observed with the appearance of the Omicron variant. Real-time PCR methodology is available for use in the majority of scientific and diagnostic institutions in Russia, whi

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de St. Maurice, Annabelle, Amy Hallmark, Evan Hilt, et al. "Implementation of Hospital-Based Candida auris Surveillance Screening Among At-Risk Patients." Infection Control & Hospital Epidemiology 41, S1 (2020): s277—s278. http://dx.doi.org/10.1017/ice.2020.846.

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Background:Candida auris is an emerging multidrug-resistant pathogen associated with outbreaks in hospitals and skilled nursing facilities (SNFs). Patients with C. auris can have invasive disease or asymptomatic colonization. Because C. auris can be difficult to treat and eradicate in the environment, the CDC recommends using contact precautions and sporicidal agents during patient care. After C. auris was identified in a patient from an LA County SNF (SNF-X), our institution initiated surveillance screening on high-risk patients. Methods: Nurses identified patients residing at SNF-X on admiss

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Yadav, Pooja, Shashi Sharma, Paban Kumar Dash, Suman Dhankher, Sandhya V. K., and S. K. Kiran. "Dry- down probe free qPCR for detection of KFD in resource limited settings." PLOS ONE 18, no. 5 (2023): e0284559. http://dx.doi.org/10.1371/journal.pone.0284559.

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Kyasanur Forest Disease is a tick-borne flavivirus is endemic in the Southern India. The recent expansion and resurgence of sporadic outbreaks in southern parts of country is the most important concern. Although only formalin inactivated vaccine is available for treatment with limited efficacy the early detection and timely identification is a only way to prevent spread of cases. If the disease can be identified prior to infection in humans like in forest areas from ticks and vectors the disease spread supposed to be managed quickly. Here we have standardized a single tube ready to use dry-dow

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Ambrose, Emmanuela E., Luke R. Smart, Mwesige Charles, et al. "Geospatial Mapping of Sickle Cell Disease in Northwest Tanzania: The Tanzania Sickle Surveillance Study (TS3)." Blood 132, Supplement 1 (2018): 3662. http://dx.doi.org/10.1182/blood-2018-99-113939.

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Abstract Tanzania ranks third in Africa for the estimated number of annual births with sickle cell disease, but these estimates are based on sparse data from small studies reported over the past 50 years. A recently completed surveillance study from Uganda documented substantial variation in the prevalence of sickle cell trait and disease across the country. Tanzania lacks a national newborn screening program, and no contemporary multi-regional screening of infants has been undertaken. We designed and conducted a prospective study to determine the prevalence of sickle cell trait and disease by

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Rud, Yu, L. Buchatsky, N. Tushnytska, and I. Hrytsyniak. "MOLECULAR DIAGNOSIS OF VIRAL DISEASES IN FISHES." Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology 22, no. 2 (2021): 323–30. http://dx.doi.org/10.36359/scivp.2021-22-2.38.

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The paper provides information about the main viral diseases of fish found in Ukraine and methods of their diagnosis. Rapid diagnosis of fish viruses using polymerase chain reaction is an affordable and relevant way to monitor and prevent outbreaks of viral diseases. The work of the Laboratory of Biotechnology in Aquaculture of the Institute of Fisheries of NAAS is aimed to develop diagnostic kits for infectious diseases of fish and conducting research in accordance with the procedure of surveillance of fish diseases. For developing diagnostic test systems, attention is paid to the trends of c

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Houang, E. T. S., Y. W. Chu, T. K. Ng, and A. F. B. Cheng. "Study of the Relatedness of Isolates ofShigella flexneri and Shigella sonnei Obtained in 1986 and 1987 and in 1994 and 1995 from Hong Kong." Journal of Clinical Microbiology 36, no. 9 (1998): 2404–7. http://dx.doi.org/10.1128/jcm.36.9.2404-2407.1998.

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We used pulsed-field gel electrophoresis (PFGE) to study the genetic relatedness of 235 isolates of Shigella flexneriand Shigella sonnei collected in Hong Kong (97 isolates from 1986 and 1987 and 138 isolates from 1994 and 1995). Altogether, 13 gels were run with bacteriophage lambda ladder DNA (Pharmacia) as an external reference in every sixth lane, standardized reagents and methods, and isolates randomized for species and years. For quantitative illustration of the relationships within a large body of isolates, computer-generated dendrograms were used to determine the number of isolates in

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Rasetti-Escargueil, Christine, and Michel R. Popoff. "Recent Developments in Botulinum Neurotoxins Detection." Microorganisms 10, no. 5 (2022): 1001. http://dx.doi.org/10.3390/microorganisms10051001.

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Botulinum neurotoxins (BoNTs) are produced as protein complexes by bacteria of the genus Clostridium that are Gram-positive, anaerobic and spore forming (Clostridium botulinum, C. butyricum, C. baratii and C. argentinense spp.). BoNTs show a high immunological and genetic diversity. Therefore, fast, precise, and more reliable detection methods are still required to monitor outbreaks and ensure surveillance of botulism. The botulinum toxin field also comprises therapeutic uses, basic research studies and biodefense issues. This review presents currently available detection methods, and new meth

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Chukwudi, Ijeoma Chekwube, Kenneth Ikejiofor Ogbu, Pam Dachung Luka, et al. "Comparison of colorimetric loop-mediated isothermal amplification kit and reverse transcription-polymerase chain reaction in the diagnosis of peste des petits ruminants in sheep and goats in Southeast Nigeria." November-2020 13, no. 11 (2020): 2358–63. http://dx.doi.org/10.14202/vetworld.2020.2358-2363.

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Background and Aim: Peste des petits ruminants (PPR) is an acute, extremely contagious transboundary viral disease of small ruminants with severe economic consequences, caused by PPR virus. Cost-effective and rapid diagnosis of the disease is essential for prompt management and control. This study aimed to compare the application of a commercial colorimetric loop-mediated isothermal amplification (cLAMP) kit and reverse transcriptase-polymerase chain reaction (RT-PCR) in the diagnosis of PPR in sheep and goats in Southeast Nigeria. Materials and Methods: Nasal swab samples were collected from

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Mwanga, Emmanuel P., Prisca A. Kweyamba, Doreen J. Siria, et al. "Reagent-free detection of Plasmodium falciparum malaria infections in field-collected mosquitoes using mid-infrared spectroscopy and machine learning." Scientific Reports 14, no. 1 (2024). http://dx.doi.org/10.1038/s41598-024-63082-z.

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AbstractField-derived metrics are critical for effective control of malaria, particularly in sub-Saharan Africa where the disease kills over half a million people yearly. One key metric is entomological inoculation rate, a direct measure of transmission intensities, computed as a product of human biting rates and prevalence of Plasmodium sporozoites in mosquitoes. Unfortunately, current methods for identifying infectious mosquitoes are laborious, time-consuming, and may require expensive reagents that are not always readily available. Here, we demonstrate the first field-application of mid-inf

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Hugo, Leon E., Karla van Huyssteen, Olamide Oloniniyi, et al. "Rapid low-resource detection of Plasmodium falciparum in infected Anopheles mosquitoes." Frontiers in Tropical Diseases 5 (January 29, 2024). http://dx.doi.org/10.3389/fitd.2024.1287025.

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Vector surveillance of Plasmodium falciparum is critical for monitoring and reducing one of the most severe forms of malaria, which causes high morbidity and mortality in children under five and pregnant women. Here we developed a rapid and highly sensitive test for the detection of P. falciparum (Pf)-infected mosquitoes (Rapid Pf test), with high suitability for low-resource vector surveillance implementation. The Rapid Pf test had similar analytical sensitivity to laboratory-based tests, detecting down to 4 copies/μL of a 18S rRNA DNA standard. In addition, the Rapid Pf test could be complet

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Perez, Julienne Sanchez, Michele Obeid, Maria Fariduddin, and Daniel Joseph Toft. "SAT546 Papillary Thyroid Cancer Surveillance: Thyroglobulin Levels Affected by HAMA." Journal of the Endocrine Society 7, Supplement_1 (2023). http://dx.doi.org/10.1210/jendso/bvad114.2017.

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Abstract Disclosure: J. Sanchez Perez: None. M. Obeid: None. M. Fariduddin: None. D.J. Toft: None. Introduction: Thyroglobulin (TG) measurement is a major means of detecting recurrence of previously treated differentiated thyroid cancers. Human-Anti Mouse Antibodies (HAMA) can interfere with lab measurements of TG levels and affect clinical decisions and treatment for these cancers.Clinical Case: A 65-year-old female was diagnosed with papillary thyroid cancer (PTC). Surgical pathology following total thyroidectomy demonstrated a tumor of 3.5 cm in its greatest dimension, unifocal, without inv

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Balea, Rickyle, Nina M. Pollak, Jody Hobson-Peters, Joanne Macdonald, and David J. McMillan. "Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings." Frontiers in Microbiology 14 (November 20, 2023). http://dx.doi.org/10.3389/fmicb.2023.1214148.

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IntroductionZika virus (ZIKV) is a re-emerging flavivirus that poses a significant public health threat. ZIKV exhibits a wide array of non-vector borne human transmission routes, such as sexual transmission, transplacental transmission and blood transfusion. Detection and surveillance of ZIKV is considered paramount in prevention of major outbreaks. With the majority of cases reported in low-resource locations, simple, low-cost detection methods are considered highly desirable.Materials and MethodsHere we have developed a sensitive and specific ZIKV diagnostic using reverse transcription recom

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Kuria, Joseph N., and Stephen M. Gathogo. "Concomitant fungal and Mycobacterium bovis infections in beef cattle in Kenya." Onderstepoort J Vet Res 80, no. 1 (2013). http://dx.doi.org/10.4102/ojvr.v80i1.585.

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Bovine tuberculosis is an important zoonosis and accurate diagnosis is important for its surveillance. Post-mortem diagnosis may, however, be compromised by lesions caused by other pathogens. In an investigation on its prevalence in slaughter cattle in Kenya, Mycobacterium bovis and dimorphic fungi were inadvertently identified separately or concurrently in tuberculous lesions. Beef carcasses were inspected for lesions in two abattoirs in Nairobi. Tissues with lesions were collected and transported to the laboratory. Smears of lesions were stained by acid-fast procedure and examined microscopi

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Hickman, Rebecca, Jason Nguyen, Tracy D. Lee, et al. "Rapid, high-throughput, cost-effective whole-genome sequencing of SARS-CoV-2 using a condensed library preparation of the Illumina DNA Prep kit." Journal of Clinical Microbiology, February 5, 2024. http://dx.doi.org/10.1128/jcm.00103-22.

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ABSTRACT The ongoing COVID-19 pandemic necessitates cost-effective, high-throughput, and timely whole-genome sequencing (WGS) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses for outbreak investigations, identifying variants of concern (VoC), characterizing vaccine breakthrough infections, and public health surveillance. In addition, the enormous demand for WGS on supply chains and the resulting shortages of laboratory supplies necessitated the use of low-reagent and low-consumable methods. Here, we report an optimized library preparation method (the BCCDC cutdown method

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Maladan, Yustinus, Hana Krismawati, Tri Wahyuni, et al. "The whole-genome sequencing in predicting Mycobacterium tuberculosis drug susceptibility and resistance in Papua, Indonesia." BMC Genomics 22, no. 1 (2021). http://dx.doi.org/10.1186/s12864-021-08139-3.

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Abstract Background Tuberculosis is one of the deadliest disease caused by Mycobacterium tuberculosis. Its treatment still becomes a burden for many countries including Indonesia. Drug resistance is one of the problems in TB treatment. However, a development in the molecular field through Whole-genome sequencing (WGS) can be used as a solution in detecting mutations associated with TB- drugs. This investigation intended to implement this data for supporting the scientific community in deeply understanding any TB epidemiology and evolution in Papua along with detecting any mutations in genes as

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Mshani, Issa H., Frank M. Jackson, Rehema Y. Mwanga, et al. "Screening of malaria infections in human blood samples with varying parasite densities and anaemic conditions using AI-Powered mid-infrared spectroscopy." Malaria Journal 23, no. 1 (2024). http://dx.doi.org/10.1186/s12936-024-05011-z.

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Abstract Background Effective testing for malaria, including the detection of infections at very low densities, is vital for the successful elimination of the disease. Unfortunately, existing methods are either inexpensive but poorly sensitive or sensitive but costly. Recent studies have shown that mid-infrared spectroscopy coupled with machine learning (MIRs-ML) has potential for rapidly detecting malaria infections but requires further evaluation on diverse samples representative of natural infections in endemic areas. The aim of this study was, therefore, to demonstrate a simple AI-powered,

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Ramos-Mandujano, Gerardo, Raik Grünberg, Yingzi Zhang, et al. "An open-source, automated, and cost-effective platform for COVID-19 diagnosis and rapid portable genomic surveillance using nanopore sequencing." Scientific Reports 13, no. 1 (2023). http://dx.doi.org/10.1038/s41598-023-47190-w.

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AbstractThe COVID-19 pandemic, caused by SARS-CoV-2, has emphasized the necessity for scalable diagnostic workflows using locally produced reagents and basic laboratory equipment with minimal dependence on global supply chains. We introduce an open-source automated platform for high-throughput RNA extraction and pathogen diagnosis, which uses reagents almost entirely produced in-house. This platform integrates our methods for self-manufacturing magnetic nanoparticles and qRT-PCR reagents-both of which have received regulatory approval for clinical use–with an in-house, open-source robotic extr

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Figueroa, Dania M., Eeva Kuisma, M. Jeremiah Matson, et al. "Development and validation of portable, field-deployable Ebola virus point-of-encounter diagnostic assay for wildlife surveillance." One Health Outlook 3, no. 1 (2021). http://dx.doi.org/10.1186/s42522-021-00041-y.

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Abstract Early detection of Ebola virus spillover into wildlife is crucial for rapid response. We developed and validated a portable, cold-chain independent Ebola virus RT-qPCR assay. Methods The field syringe-based RNA extraction method was compared with a conventional laboratory-based spin-column RNA extraction method. Next, the qPCR efficiency and limit of detection of the assay was compared to standard laboratory-based reagents and equipment. The specificity of the assay was confirmed by testing against multiple Zaire Ebolavirus (EBOV) variants and other ebolavirus species. Lastly, swabs f

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