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Artigos de revistas sobre o assunto "Plasmids"

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Lopez, Jaime G., Mohamed S. Donia, and Ned S. Wingreen. "Modeling the ecology of parasitic plasmids." ISME Journal 15, no. 10 (2021): 2843–52. http://dx.doi.org/10.1038/s41396-021-00954-6.

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AbstractPlasmids are autonomous genetic elements that can be exchanged between microorganisms via horizontal gene transfer (HGT). Despite the central role they play in antibiotic resistance and modern biotechnology, our understanding of plasmids’ natural ecology is limited. Recent experiments have shown that plasmids can spread even when they are a burden to the cell, suggesting that natural plasmids may exist as parasites. Here, we use mathematical modeling to explore the ecology of such parasitic plasmids. We first develop models of single plasmids and find that a plasmid’s population dynami
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Lopez-Diaz, Maria, Nicholas Ellaby, Jane Turton, Neil Woodford, Maria Tomas, and Matthew J. Ellington. "NDM-1 carbapenemase resistance gene vehicles emergent on distinct plasmid backbones from the IncL/M family." Journal of Antimicrobial Chemotherapy 77, no. 3 (2022): 620–24. http://dx.doi.org/10.1093/jac/dkab466.

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Abstract Objectives To assess the genetic contexts surrounding blaNDM-1 genes carried on IncM plasmids harboured by six carbapenemase-producing Enterobacterales (CPE) isolates referred to the UK Health Security Agency’s Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit. Methods Between 2014 and 2018, the AMRHAI Reference Unit undertook WGS of CPE isolates using Illumina NGS. Nanopore sequencing was used for selected isolates and publicly available plasmid references were downloaded. Analysis of incRNA, which encodes the antisense RNA regulating plasmidic rep
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Li, Feifeng, Jiong Wang, Ying Jiang, et al. "Adaptive Evolution Compensated for the Plasmid Fitness Costs Brought by Specific Genetic Conflicts." Pathogens 12, no. 1 (2023): 137. http://dx.doi.org/10.3390/pathogens12010137.

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New Delhi metallo-β-lactamase (NDM)-carrying IncX3 plasmids is important in the transmission of carbapenem resistance in Escherichia coli. Fitness costs related to plasmid carriage are expected to limit gene exchange; however, the causes of these fitness costs are poorly understood. Compensatory mutations are believed to ameliorate plasmid fitness costs and enable the plasmid’s wide spread, suggesting that such costs are caused by specific plasmid–host genetic conflicts. By combining conjugation tests and experimental evolution with comparative genetic analysis, we showed here that the fitness
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Bahl, Martin Iain, Lars Hestbjerg Hansen, Tine Rask Licht, and Søren J. Sørensen. "Conjugative Transfer Facilitates Stable Maintenance of IncP-1 Plasmid pKJK5 in Escherichia coli Cells Colonizing the Gastrointestinal Tract of the Germfree Rat." Applied and Environmental Microbiology 73, no. 1 (2006): 341–43. http://dx.doi.org/10.1128/aem.01971-06.

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ABSTRACT Quantitative determination of IncP-1 plasmid loss from Escherichia coli cells colonizing the gastrointestinal tracts of germfree rats was achieved by flow cytometry. Results show that the plasmid's ability to conjugate counteracts plasmid loss and is thus an important mechanism for the stable maintenance of IncP-1 plasmids within the gastrointestinal environment.
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Longtine, M. S., S. Enomoto, S. L. Finstad, and J. Berman. "Yeast telomere repeat sequence (TRS) improves circular plasmid segregation, and TRS plasmid segregation involves the RAP1 gene product." Molecular and Cellular Biology 12, no. 5 (1992): 1997–2009. http://dx.doi.org/10.1128/mcb.12.5.1997-2009.1992.

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Telomere repeat sequences (TRSs) can dramatically improve the segregation of unstable circular autonomously replicating sequence (ARS) plasmids in Saccharomyces cerevisiae. Deletion analysis demonstrated that yeast TRSs, which conform to the general sequence (C(1-3)A)n, are able to stabilize circular ARS plasmids. A number of TRS clones of different primary sequence and C(1-3)A tract length confer the plasmid stabilization phenotype. TRS sequences do not appear to improve plasmid replication efficiency, as determined by plasmid copy number analysis and functional assays for ARS activity. Pedig
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Longtine, M. S., S. Enomoto, S. L. Finstad, and J. Berman. "Yeast telomere repeat sequence (TRS) improves circular plasmid segregation, and TRS plasmid segregation involves the RAP1 gene product." Molecular and Cellular Biology 12, no. 5 (1992): 1997–2009. http://dx.doi.org/10.1128/mcb.12.5.1997.

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Telomere repeat sequences (TRSs) can dramatically improve the segregation of unstable circular autonomously replicating sequence (ARS) plasmids in Saccharomyces cerevisiae. Deletion analysis demonstrated that yeast TRSs, which conform to the general sequence (C(1-3)A)n, are able to stabilize circular ARS plasmids. A number of TRS clones of different primary sequence and C(1-3)A tract length confer the plasmid stabilization phenotype. TRS sequences do not appear to improve plasmid replication efficiency, as determined by plasmid copy number analysis and functional assays for ARS activity. Pedig
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Pollet, Rebecca M., James D. Ingle, Jeff P. Hymes, et al. "Processing of Nonconjugative Resistance Plasmids by Conjugation Nicking Enzyme of Staphylococci." Journal of Bacteriology 198, no. 6 (2016): 888–97. http://dx.doi.org/10.1128/jb.00832-15.

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ABSTRACTAntimicrobial resistance inStaphylococcus aureuspresents an increasing threat to human health. This resistance is often encoded on mobile plasmids, such as pSK41; however, the mechanism of transfer of these plasmids is not well understood. In this study, we first examine key protein-DNA interactions formed by the relaxase enzyme, NES, which initiates and terminates the transfer of the multidrug resistance plasmid pSK41. Two loops on the NES protein, hairpin loops 1 and 2, form extensive contacts with the DNA hairpin formed at theoriTregion of pSK41, and here we establish that these con
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Brown, Celeste J., Diya Sen, Hirokazu Yano, et al. "Diverse Broad-Host-Range Plasmids from Freshwater Carry Few Accessory Genes." Applied and Environmental Microbiology 79, no. 24 (2013): 7684–95. http://dx.doi.org/10.1128/aem.02252-13.

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ABSTRACTBroad-host-range self-transferable plasmids are known to facilitate bacterial adaptation by spreading genes between phylogenetically distinct hosts. These plasmids typically have a conserved backbone region and a variable accessory region that encodes host-beneficial traits. We do not know, however, how well plasmids that do not encode accessory functions can survive in nature. The goal of this study was to characterize the backbone and accessory gene content of plasmids that were captured from freshwater sources without selecting for a particular phenotype or cultivating their host. T
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Wu, Shang Wei, Kathrine Dornbusch, Göran Kronvall та Mari Norgren. "Characterization and Nucleotide Sequence of a Klebsiella oxytoca Cryptic Plasmid Encoding a CMY-Type β-Lactamase: Confirmation that the Plasmid-Mediated Cephamycinase Originated from the Citrobacter freundii AmpC β-Lactamase". Antimicrobial Agents and Chemotherapy 43, № 6 (1999): 1350–57. http://dx.doi.org/10.1128/aac.43.6.1350.

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ABSTRACT Plasmid pTKH11, originally obtained by electroporation of aKlebsiella oxytoca plasmid preparation intoEscherichia coli XAC, expressed a high level of an AmpC-like β-lactamase. The enzyme, designated CMY-5, conferred resistance to extended-spectrum β-lactams in E. coli; nevertheless, the phenotype was cryptic in the K. oxytocadonor. Determination of the complete nucleotide sequence of pTKH11 revealed that the 8,193-bp plasmid encoded seven open reading frames, including that for the CMY-5 β-lactamase (bla CMY-5). Thebla CMY-5 product was similar to the plasmidic CMY-2 β-lactamase of K.
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Paganini, Julian A., Nienke L. Plantinga, Sergio Arredondo-Alonso, Rob J. L. Willems, and Anita C. Schürch. "Recovering Escherichia coli Plasmids in the Absence of Long-Read Sequencing Data." Microorganisms 9, no. 8 (2021): 1613. http://dx.doi.org/10.3390/microorganisms9081613.

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The incidence of infections caused by multidrug-resistant E. coli strains has risen in the past years. Antibiotic resistance in E. coli is often mediated by acquisition and maintenance of plasmids. The study of E. coli plasmid epidemiology and genomics often requires long-read sequencing information, but recently a number of tools that allow plasmid prediction from short-read data have been developed. Here, we reviewed 25 available plasmid prediction tools and categorized them into binary plasmid/chromosome classification tools and plasmid reconstruction tools. We benchmarked six tools (MOB-su
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Teses / dissertações sobre o assunto "Plasmids"

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Jansen, Yvette. "Characterisation of a high copy number mutant pAL5000 origin of replication." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52159.

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Thesis (MScMedSc)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: The plasmid pAL5000 is a mycobacterial plasmid isolated from Mycobacterium fortuitum. It is a low copy number plasmid, which replicates in both fast growing (e.g. M. smegmatis) and slow growing (e.g. M. bovis BCG) mycobacteria. Most mycobacterial-E. coli shuttle vectors utilise the pAL5000 origin of replication. The minimum replicon consists of ORF1 (RepA), ORF2 (RepB) and the origin of replication. Dr W.R. Bourn created an E. coli-mycobacterial vector based on the pAL5000 origin of replication (pORI) and then subje
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Udo, Edet Ekpenyong. "Characterisation and molecular studies of plasmids from Nigerian staphylococci." Thesis, Curtin University, 1991. http://hdl.handle.net/20.500.11937/1845.

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Fifty three Staphylococcus aureus isolates were obtained from three centres, two hospitals and a private pathology laboratory, and studied for susceptibility to bacteriophages, resistance to antimicrobial agents and plasmid contents.Results of bacteriophage typing revealed that they belonged to a variety of phage types. Eighteen were untypable by any of the International Set of Phages, 16 belonged to phage group 111, nine to group I, four to group 11, two to group IV and two to the miscellaneous group.The isolates were resistant to one or more of methicillin (Mc), benzyl penicillin (Pc), genta
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Seyler, Richard W. "Plasmid stability of pUB110 and pUB110-derived plasmids in Bacillus sphaericus 2362." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-03022010-020139/.

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Hirst, Jonathan Michael. "Plasmids in thermoactinomyces." Thesis, Heriot-Watt University, 1991. http://hdl.handle.net/10399/826.

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Udo, Edet Ekpenyong. "Characterisation and molecular studies of plasmids from Nigerian staphylococci." Curtin University of Technology, School of Biomedical Sciences, 1991. http://espace.library.curtin.edu.au:80/R/?func=dbin-jump-full&object_id=15648.

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Fifty three Staphylococcus aureus isolates were obtained from three centres, two hospitals and a private pathology laboratory, and studied for susceptibility to bacteriophages, resistance to antimicrobial agents and plasmid contents.Results of bacteriophage typing revealed that they belonged to a variety of phage types. Eighteen were untypable by any of the International Set of Phages, 16 belonged to phage group 111, nine to group I, four to group 11, two to group IV and two to the miscellaneous group.The isolates were resistant to one or more of methicillin (Mc), benzyl penicillin (Pc), genta
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Ophel, Kathleen Margaret. ""Agrobacterium" : plasmids and biovars /." Title page, contents and summary only, 1987. http://web4.library.adelaide.edu.au/theses/09PH/09pho61.pdf.

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Pinder, David. "Illegitimate recombination in plasmids." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/11260.

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Illegitimate recombination mechanisms are important for genetic change within an organism. They are also the cause of many instability problems in biotechnology and have been associated with certain human genetic disorders and cancers. The original aim of this work was to construct a deletion (illegitimate recombination) resistant cosmid based, cloning system, for the cloning of unstable human DNA. Two 'mutant plasmids' pMS5 and pMS7 were isolated. The plasmids were derived from pUC18 and appeared to stabilise the propagation of a long DNA palindrome. The basic concept was to construct a new c
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Maia, Mauricio Silva. "Plasmids of Azotobacter vinelandii." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc798298/.

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Nineteen laboratory strains of Azotobacter vinelandii and two organisms of the same species isolated from water samples were screened for the presence of plasmid deoxyribonucleic acid (DNA). Three laboratory strains and both organisms isolated from water samples contained one plasmid each. The migration distances of the plasmids in agarose gel electrophoresis were different molecular weights. The plasmids were cured by SDS or ethidium bromide treatment of the cultures.
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Chilley, Paul Morris. "Leading regions of enterobacterial plasmids." Thesis, University of Leicester, 1995. http://hdl.handle.net/2381/34394.

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Gram-negative bacterial conjugation is a specialised replicative event that increases the population size of a plasmid during its horizontal transfer between organisms. Work reported in this thesis has focused on the genetic structure of the leading regions of enterobacterial plasmids. A leading region is the first segment of a plasmid to enter the recipient cell during conjugation. Some leading regions carry conserved loci. Examples are ssb encoding a single-stranded DNA binding protein; psiB which functions to inhibit induction of the bacterial SOS response; ardA which encodes antirestrictio
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McKibben, Ann Laura. "Characterization of plasmids in Gluconobacter." Thesis, Virginia Tech, 1992. http://hdl.handle.net/10919/44232.

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Livros sobre o assunto "Plasmids"

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Tolmasky, Marcelo E., and Juan C. Alonso, eds. Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.

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Helinski, Donald R., Stanley N. Cohen, Don B. Clewell, David A. Jackson, and Alexander Hollaender, eds. Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8.

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Meinhardt, Friedhelm, and Roland Klassen, eds. Microbial Linear Plasmids. Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-72025-6.

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Esser, Karl, Ulrich Kück, Christine Lang-Hinrichs, et al. Plasmids of Eukaryotes. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-82585-9.

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Friedhelm, Meinhardt, and Klassen Roland, eds. Microbial linear plasmids. Berlin, 2007.

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R, Helinski Donald, ed. Plasmids in bacteria. Plenum Press, 1985.

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B, Clewell Don, ed. Bacterial conjugation. Plenum Press, 1993.

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G, Hardy K., ed. Plasmids: A practical approach. IRL, 1986.

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G, Hardy K., ed. Plasmids: A practical approach. IRL Press, 1987.

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G, Hardy K., ed. Plasmids: A practical approach. 2nd ed. IRL Press at Oxford University Press, 1993.

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Capítulos de livros sobre o assunto "Plasmids"

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Inouye, Sachiye. "Plasmids." In Pseudomonas. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-0120-0_1.

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Gooch, Jan W. "Plasmids." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14515.

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Falkinham, Joseph O., and Jack T. Crawford. "Plasmids." In Tuberculosis. ASM Press, 2014. http://dx.doi.org/10.1128/9781555818357.ch13.

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Jannière, Laurent, Alexandra Gruss, and S. Dusko Ehrlich. "Plasmids." In Bacillus subtilis and Other Gram-Positive Bacteria. ASM Press, 2014. http://dx.doi.org/10.1128/9781555818388.ch43.

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Tolmasky, Marcelo E., Luis A. Actis, Timothy J. Welch, and Jorge H. Crosa. "Plasmids." In Methods for General and Molecular Microbiology. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817497.ch30.

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Kado, Clarence I. "Historical Events That Spawned the Field of Plasmid Biology." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch1.

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Hernández-Arriaga, Ana María, Wai Ting Chan, Manuel Espinosa, and Ramón Díaz-Orejas. "Conditional Activation of Toxin-Antitoxin Systems: Postsegregational Killing and Beyond." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch10.

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Volante, Andrea, Nora E. Soberón, Silvia Ayora, and Juan C. Alonso. "The Interplay between Different Stability Systems Contributes to Faithful Segregation: Streptococcus pyogenes pSM19035 as a Model." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch11.

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Samson, Julie E., Alfonso H. Magadan, and Sylvain Moineau. "The CRISPR-Cas Immune System and Genetic Transfers: Reaching an Equilibrium." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch12.

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de Toro, María, M. Pilar Garcillán-Barcia, and Fernando de la Cruz. "Plasmid Diversity and Adaptation Analyzed by Massive Sequencing of Escherichia coli Plasmids." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch13.

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Trabalhos de conferências sobre o assunto "Plasmids"

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Yoshida, Subaru, Tongyao Li, Takayuki Umakoshi, and Prabhat Verma. "Unidirectional plasmons propagation." In JSAP-Optica Joint Symposia. Optica Publishing Group, 2024. https://doi.org/10.1364/jsapo.2024.19a_p08_8.

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To excite surface plasmons efficiently, the surface plasmons must receive energy from incident light, in which conservation of momentum must be satisfied between light propagating in free space and the surface plasmons. However, the momentum of light propagating through air never matches the momentum of surface plasmons. One solution is to use a grating as a plasmon coupler. This allows the momentum of the incident light to match the momentum of the surface plasmons for efficient surface plasmon excitation. However, in this method, the direction of propagation of surface plasmons cannot be con
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A., Klimov. "Plasmoid Tornado." In 2004_Wroclaw. IEEE, 2004. https://doi.org/10.23919/emc.2004.10844204.

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Saksaganskaia, Alla S., Victoria S. Muntyan, Alexey N. Muntyan, Boris V. Simarov, and Marina L. Roumiantseva. "ABUNDANCE OF PHAGE-RELATED SEQUENCES ON NON-SYMBIOTIC PLASMIDS OF SINORHIZOBIUM MELILOTI FROM CENTERS OF LEGUME PLANTS DIVERSITY." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/6.1/s25.06.

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Genomes of alfalfa root nodule bacteria, Sinorhizobium meliloti, symbionts of alfalfa are enriched in non-symbiotic (cryptic) plasmids, which gene pool is remained weakly studied. S. meliloti strains are significantly varied in number and size of these plasmids. The goal of the study was to assess the occurrence of phage-related sequences (PRS) on cryptic plasmids. Whole genome sequences of 12 S. meliloti strains native to Caucasian and Kazakhstan centers of alfalfa diversity (NCG and PAG, correspondingly) were studied and 20 cryptic plasmids, which sizes varied from 17.2 to 453.8 kb, were ass
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Kulikov, V. V., D. N. Shcherbakov, and E. A. Kolosova. "GETTING A GENETICALLY MODIFIED STRAIN OF E. COLI EXPRESSING THE SACB SACCHARASE GENE WITH THE USE OF MOBILE GENETIC ELEMENTS." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-252.

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A number of DNA vaccines derives from plasmids. Antibiotic resistance is used for selection and containment of plasmid DNA in microorganisms. However production requirements of vaccine exclude of antibiotics and resistant microorganisms. As an alternative marker the enzyme sucrose can be used. A genetically modified strain of E. coli with sacB gene expression, which is capable of providing antibiotic-free breeding was obtained by genetic engineering using CRISPR/Cas.
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Paulsson, Johan. "Plasmids as stochastic model systems." In SPIE's First International Symposium on Fluctuations and Noise, edited by Sergey M. Bezrukov, Hans Frauenfelder, and Frank Moss. SPIE, 2003. http://dx.doi.org/10.1117/12.500143.

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Ezerskii, V. A., E. M. Koloskova, and T. P. Trubitsina. "Green fluorescent protein gene for site-specific integration into the locus of the rabbit whey acidic protein gene." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-129.

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The high content of whey acidic protein in rabbit milk makes the gene of this protein a promising candidate for its replacement by the gene of pharmacologically active protein using the CRISPR/Cas9 system. The plasmid that contains 5’ and 3’ arms of homology to the rabbit WAP gene was created. A fragment containing a green fluorescent protein gene under the CMV promoter has been integrated into this site. A strategy of making double-stranded cuts in the gene WAP and receiving four pX330 plasmids encoding the endonuclease Cas9 and guide RNAs was developed. The plasmid containing a fragment cmvE
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Vladimirova, Mariia, Maria Vladimirova, Alexey Afonin, Boris Simarov, and Marina Roumiantseva. "CRYPTIC PLASMIDS ESSENTIAL FOR SINORHIZOBIUM MELILOTI FITNESS." In 20th International Multidisciplinary Scientific GeoConference Proceedings SGEM 2020. STEF92 Technology, 2020. http://dx.doi.org/10.5593/sgem2020/6.1/s25.029.

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Alwa, Amira, and Samir Jaoua. "Investigation of Bacillus Thuringiensis Plasmid Instability and its Effect on the Synthesis of Crystals." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0107.

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In order to explore plasmid instability in Bt, four Bt strains belonging to two Bt subspecies were cultured at 42°C for 9 days. HD1 and QBT376 belong to subspecies kurstaki, while H14 and QBT218 belong to subspecies israelensis. Results showed 100% crystal loss for H14 and QBT218, while 76% and 90% crystal loss for HD1 and QBT376, respectively, showing that cry-carrying plasmids are more stable in Bt kurs. than in Bt isr.. HD1, QBT376, and QBT218 cured clones showed significant protease activity compared to their non-cured counterparts. Microscopic observation revealed the delay of sporulation
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Ratnadewi, Anak Agung Istri, Sabella Muyasyaroh, Fatih Harum, Wuryanti Handayani та Sudarko Sudarko. "Expression and Characterization of Recombinant Endo-β-1,4-D-xylanases XynBTN63D from Soil Termite Abdomen in <i>Escherichia coli</i> BL21 (DE3)". У International Conference on Chemistry and Material Sciences 2023 (IC2MS). Trans Tech Publications Ltd, 2024. http://dx.doi.org/10.4028/p-5cqsmp.

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The xynBTN63D gene sub-cloned on the plasmid shuttle vector pESC and pYHM1 in the host Escherichia coli BL21 (DE3) was successfully expressed and characterized. The xynBTN63D gene in the soluble fraction of each plasmid is expressed at induction temperatures of 25, 30, 35, 37, and 40 °C with a molecular weight of ±30 kDa. The soluble fraction of the xynBTN63D gene in both plasmids was expressed at induction temperatures of 25, 30, 35, 37, and 40°C with a molecular weight of ±30 kDa. The recombinant XynBTN63D, purified using the fast protein liquid chromatography (FPLC) method also has a molecu
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Ibragimov, A., An Baymiev, and O. Lastochkina. "Development of fluorescent protein-marked strains of Bacillus subtilis." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.104.

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Relatórios de organizações sobre o assunto "Plasmids"

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Crocker, Fiona, Lyndsay Carrigee, Kayla Clark, and Karl Indest. Peptide display for rare earth element binding. Engineer Research and Development Center (U.S.), 2025. https://doi.org/10.21079/11681/49647.

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Rare earth elements (REEs) are metals that are indispensable to the function of many advanced systems and materials. The supply chain of REEs is heavily dependent on foreign sources and supply shortages are a major concern to the US government. Biological recovery approaches could be an economically feasible approach to recover REEs from unconventional or secondary sources. The objective of this project was to express a lanthanide-binding tag, with an affinity for adsorption of REEs, on the surface of the biomining bacterium, Acidithiobacillus ferrooxidans. This was to be accomplished using sy
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Top, Eva M., and Ben Ridenhour. Persistence of Antibiotic Resistance Plasmids in Biofilms. Defense Technical Information Center, 2014. http://dx.doi.org/10.21236/ada614277.

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Plumley, F. G. Marine Diatom Plasmids and their Biotechnological Applications. Defense Technical Information Center, 1992. http://dx.doi.org/10.21236/ada264407.

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Top, Eva M., and Silvia E. Smith. Persistence of Antibiotic Resistance Plasmids in Biofilms. Defense Technical Information Center, 2013. http://dx.doi.org/10.21236/ada615372.

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Coons, Terry. Restriction mapping and expression of recombinant plasmids containing the arsenic resistance genes of the plasmid R45. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.5481.

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Eisen, Jonathan. Shotgun Sequencing of Plasmids from Marine Sediment Bacteria - Genetic Exploration. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada398735.

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Hamilton, Nicklas. Use of Two-replisome Plasmids to Characterize how Chromosome Replication Completes. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.6940.

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Clark, Joshua. Determination of homology between the arsenic resistance plasmids R45 and R773 in Escherichia coli. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.5644.

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Lindow, Steven, Isaac Barash, and Shulamit Manulis. Relationship of Genes Conferring Epiphytic Fitness and Internal Multiplication in Plants in Erwinia herbicola. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573065.bard.

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Most bacterial plant pathogens colonize the surface of healthy plants as epiphytes before colonizing internally and initiating disease. The epiphytic phase of these pathogens is thus an important aspect of their epidemiology and a stage at which chemical and biological control is aimed. However, little is known of the genes and phenotypes that contribute to the ability of bacteria to grow on leaves and survive the variable physical environment in this habitat. In addition, while genes such as hrp awr and others which confer pathogenicity and in planta growth ability have been described, their
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Singh, Anjali. What Is Optogenetics and How Does It Work? ConductScience, 2022. http://dx.doi.org/10.55157/cs20220704.

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Optogenetics is a biotechnological method that combines optical systems and genetic engineering to control and monitor the functions of cells, tissues, and organisms. It involves using light-sensitive proteins called opsins to manipulate specific cells or regions with precision. This technique has revolutionized neuroscience, allowing researchers to study neural circuits and behavior by turning cells on and off. Opsins are categorized into microbial and animal types, each with specific functions. Optogenetic experiments require opsins, suitable plasmids or viral vectors, and a light source. Th
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