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Teses / dissertações sobre o tema "Plasmids"

Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 25 de julho de 2025

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1

Jansen, Yvette. "Characterisation of a high copy number mutant pAL5000 origin of replication." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52159.

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Thesis (MScMedSc)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: The plasmid pAL5000 is a mycobacterial plasmid isolated from Mycobacterium fortuitum. It is a low copy number plasmid, which replicates in both fast growing (e.g. M. smegmatis) and slow growing (e.g. M. bovis BCG) mycobacteria. Most mycobacterial-E. coli shuttle vectors utilise the pAL5000 origin of replication. The minimum replicon consists of ORF1 (RepA), ORF2 (RepB) and the origin of replication. Dr W.R. Bourn created an E. coli-mycobacterial vector based on the pAL5000 origin of replication (pORI) and then subje

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2

Udo, Edet Ekpenyong. "Characterisation and molecular studies of plasmids from Nigerian staphylococci." Thesis, Curtin University, 1991. http://hdl.handle.net/20.500.11937/1845.

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Fifty three Staphylococcus aureus isolates were obtained from three centres, two hospitals and a private pathology laboratory, and studied for susceptibility to bacteriophages, resistance to antimicrobial agents and plasmid contents.Results of bacteriophage typing revealed that they belonged to a variety of phage types. Eighteen were untypable by any of the International Set of Phages, 16 belonged to phage group 111, nine to group I, four to group 11, two to group IV and two to the miscellaneous group.The isolates were resistant to one or more of methicillin (Mc), benzyl penicillin (Pc), genta

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3

Seyler, Richard W. "Plasmid stability of pUB110 and pUB110-derived plasmids in Bacillus sphaericus 2362." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-03022010-020139/.

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4

Hirst, Jonathan Michael. "Plasmids in thermoactinomyces." Thesis, Heriot-Watt University, 1991. http://hdl.handle.net/10399/826.

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5

Udo, Edet Ekpenyong. "Characterisation and molecular studies of plasmids from Nigerian staphylococci." Curtin University of Technology, School of Biomedical Sciences, 1991. http://espace.library.curtin.edu.au:80/R/?func=dbin-jump-full&object_id=15648.

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Fifty three Staphylococcus aureus isolates were obtained from three centres, two hospitals and a private pathology laboratory, and studied for susceptibility to bacteriophages, resistance to antimicrobial agents and plasmid contents.Results of bacteriophage typing revealed that they belonged to a variety of phage types. Eighteen were untypable by any of the International Set of Phages, 16 belonged to phage group 111, nine to group I, four to group 11, two to group IV and two to the miscellaneous group.The isolates were resistant to one or more of methicillin (Mc), benzyl penicillin (Pc), genta

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6

Ophel, Kathleen Margaret. ""Agrobacterium" : plasmids and biovars /." Title page, contents and summary only, 1987. http://web4.library.adelaide.edu.au/theses/09PH/09pho61.pdf.

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7

Pinder, David. "Illegitimate recombination in plasmids." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/11260.

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Illegitimate recombination mechanisms are important for genetic change within an organism. They are also the cause of many instability problems in biotechnology and have been associated with certain human genetic disorders and cancers. The original aim of this work was to construct a deletion (illegitimate recombination) resistant cosmid based, cloning system, for the cloning of unstable human DNA. Two 'mutant plasmids' pMS5 and pMS7 were isolated. The plasmids were derived from pUC18 and appeared to stabilise the propagation of a long DNA palindrome. The basic concept was to construct a new c

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8

Maia, Mauricio Silva. "Plasmids of Azotobacter vinelandii." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc798298/.

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Nineteen laboratory strains of Azotobacter vinelandii and two organisms of the same species isolated from water samples were screened for the presence of plasmid deoxyribonucleic acid (DNA). Three laboratory strains and both organisms isolated from water samples contained one plasmid each. The migration distances of the plasmids in agarose gel electrophoresis were different molecular weights. The plasmids were cured by SDS or ethidium bromide treatment of the cultures.

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9

Chilley, Paul Morris. "Leading regions of enterobacterial plasmids." Thesis, University of Leicester, 1995. http://hdl.handle.net/2381/34394.

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Gram-negative bacterial conjugation is a specialised replicative event that increases the population size of a plasmid during its horizontal transfer between organisms. Work reported in this thesis has focused on the genetic structure of the leading regions of enterobacterial plasmids. A leading region is the first segment of a plasmid to enter the recipient cell during conjugation. Some leading regions carry conserved loci. Examples are ssb encoding a single-stranded DNA binding protein; psiB which functions to inhibit induction of the bacterial SOS response; ardA which encodes antirestrictio

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10

McKibben, Ann Laura. "Characterization of plasmids in Gluconobacter." Thesis, Virginia Tech, 1992. http://hdl.handle.net/10919/44232.

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11

McKibben, Laura Ann. "Characterization of plasmids in Gluconobacter /." This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-08142009-040503/.

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12

Cook, Marisa Anne. "Replicons derived from endogenously isolated plasmids used to classify plasmids occurring in marine sediment bacteria." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/25736.

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13

Asghari, Abdolkarim. "Physical Characterization and Restriction Mapping of the Sal Plasmid From Pseudomonas Putida." Thesis, North Texas State University, 1987. https://digital.library.unt.edu/ark:/67531/metadc798475/.

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Physical and restriction mapping of the salicylate catabolic plasmid SAL from Pseudomonas putida strain PpG 2119 was carried out by standard multiple restriction analysis and by cross hybridization studies using radioactively labeled restriction fragment probes. The total numbers of fragments produced, their respective sizes, the arrangement of the restriction fragments on the plasmid and the map locations of the enzyme recognition sites for Hpal, Xhol, Dral and Smal are given.

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14

Zainuddin, Zainul Fadziruddin. "Mycobacterial plasmids and related DNA sequences." Thesis, University of Surrey, 1988. http://epubs.surrey.ac.uk/843201/.

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An investigation into the presence of plasmids in Mycobacterium tuberculosis was conducted. No extrachromosomal DNA was found although some evidence for their presence were detected. Plasmid-related sequences were however found in the genomic DNA of M, tuberculosis. Isolation of plasmid-related sequences from a strain of M, tuberculosis resulted in two different DNA probes which showed high specificity for M, tuberculosis and related species. One of these probes gave banding patterns which suggest the presence of restriction fragment polymorphism in M, tuberculosis. The other probe gave bandin

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15

Walters, John Anthony. "Replication of plasmids of Staphylococcus aureus." Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315767.

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16

Winson, Michael Kenneth. "Molecular biological studies on catabolic plasmids." Thesis, Bangor University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305927.

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17

Thomas, Nicholas Simon. "The molecular biology of chlamydial plasmids." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295857.

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18

Saw, Howard Thien Hui. "The biology of antibiotic resistance plasmids." Thesis, University of Birmingham, 2015. http://etheses.bham.ac.uk//id/eprint/6083/.

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Plasmids confer genes encoding clinically relevant antibiotic resistance. It was hypothesised that the AcrAB-TolC multidrug resistance efflux pump was required for clinically relevant levels of carbapenem resistance. However, carbapenemase-producing \(Salmonella\) TolC mutants were less susceptible to carbapenems. In the presence of the efflux inhibitor phe-arg- β-naphthylamide (PAβN), wildtype bacteria and 36/86 non-replicate clinical isolates of carbapenem-producing Enterobacteriaceae were ≥4-fold less susceptible to ertapenem. Experimental data suggested that OmpF repression conferred the i

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19

Taylor, James Andrew. "The plasmids of Rhodococcus aetherivorans I24." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613971.

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20

Coons, Terry M. "Restriction mapping and expression of recombinant plasmids containing the arsenic resistance genes of the plasmid R45." PDXScholar, 1986. https://pdxscholar.library.pdx.edu/open_access_etds/3597.

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The trivalent (arsenite) and pentavalent (arsenate) forms of arsenic are introduced into the environment through the use of arsenic in herbicides, pesticides, fertilizers, and the smelting of arsenic-bearing ores. Bacteria resistant to arsenic are readily isolated from surface waters, sewage, and clinical infections. Although some bacterial resistance is provided by inducible phosphate transport systems that discriminate against arsenate, marked resistance is carried on bacterial plasmids. A 6.9 kilobase fragment previously derived from one such plasmid, R45, and containing the genes for induc

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21

Lazdins, Alessandro. "Development of plasmids that can displace antibiotic resistance plasmids from bacteria in the human and animal gastrointestinal tract." Thesis, University of Birmingham, 2018. http://etheses.bham.ac.uk//id/eprint/7990/.

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Antibiotic resistance is one of the greatest scientific and medical challenges of the 21st century. However, our arsenal to combat this surge is limited to a small number of antimicrobial drugs. Plasmids are major contributors to the problem, yet specifically targeting them to reduce the burden of resistant infections has not been fully exploited. It is possible to repress plasmid’s replication and eliminate them from a population by harnessing their innate replication and maintenance functions. This concept has been applied in this study to construct conjugative broad-host range plasmids aime

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22

Catchpole, Ian R. "Studies of small plasmids of Staphylococcus aureus." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253298.

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23

Harrison, Peter William. "Bacterial chromids are neither chromosomes nor plasmids." Thesis, University of York, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.556257.

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In addition to the main chromosome, many bacterial genomes have a second element, often called a "second chromosome" or "megaplasmid". It has become clear that, while they carry some essential genes, these replicons do not have the properties normally expected of chromosomes. In this thesis a systematic analysis of all publicly available sequenced bacterial genomes demonstrates that these large secondary replicons represent a single class of elements with a distinct and consistent set of properties. The term 'chromid' is proposed to distinguish them from both chromosomes and plasmids. Chapter

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24

Chak, K.-F. "Expression and regulation of Col E plasmids." Thesis, University of East Anglia, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370379.

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25

Lyle, Keenan Harris. "Comparison of plasmids from clinical Lactobacillus strains." University of the Western Cape, 2018. http://hdl.handle.net/11394/6397.

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Magister Scientiae - MSc (Biotechnology)<br>The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has been found to be the predominant Lactobacillus species. Many studies have been conducted to assess factors influencing lactobacilli dominance in the vaginal microbiome. However, no study has evaluated the impact of plasmids on the vaginal lactobacilli. In the present study two plasmids, pLc17 and pLc4, isolated from vaginal Lactobacil

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26

Pinto, Beatriz Lázaro. "Broad host range plasmids in estuarine environments." Master's thesis, Universidade de Aveiro, 2010. http://hdl.handle.net/10773/3515.

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Mestrado em Biotecnologia<br>A transferência horizontal de genes permite a adaptação microbiana a nichos especiais, dos quais dois bons exemplos são a camada superficial do mar e a coluna de água. Os plasmídeos com gama alargada de hospedeiros (BHR), responsáveis pelo fluxo de material genético entre cromossomas bacterianos (inclusivamente entre microorganismos muito afastados filogeneticamente) têm um papel essencial na evolução das comunidades microbianas. Entre eles, os plasmídeos pertencendo ao grupo de incompatibilidade IncP-1 têm um interesse especial por causa da sua extraordinár

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27

El-Haffar, Mohammad I. A. "The survival of bacteria containing r-plasmids." Thesis, Aston University, 1985. http://publications.aston.ac.uk/12451/.

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28

Kawasaki, Haruhiko. "STUDIES ON PLASMIDS DETERMINING BACTERIAL HALOACETATE DEHALOGENASES." Kyoto University, 1985. http://hdl.handle.net/2433/78183.

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29

Bott, Martha Anne Brunner David P. "Growth inhibition mediated by E4 colicin plasmids." Normal, Ill. Illinois State University, 1986. http://wwwlib.umi.com/cr/ilstu/fullcit?p8626588.

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Thesis (Ph. D.)--Illinois State University, 1986.<br>Title from title page screen, viewed July 13, 2005. Dissertation Committee: David P. Brunner (chair), Herman E. Brockman, Arlan G. Richardson, H. Tak Cheung, Lynne Lucher. Includes bibliographical references (leaves 167-183) and abstract. Also available in print.

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30

Sanders, Rhiannon. "Segregational stability of plasmids in Bacillus subtilis." Thesis, University of Warwick, 1986. http://wrap.warwick.ac.uk/109832/.

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The stability of plasmids in Bacillus subtilis in the absence of selective pressure under long term chemostat culture and under different conditions of nutrient limitation was investigated. Culture and sampling conditions were established to allow the detection of plasmid-free cells and samples were routinely screened in order to monitor the structural integrity of the resident plasmid. Initially the stability of the plasmid pHV14-F (carrying the repressor gene of phage 0105) in the Bacillus subtilis strain 3G18 was investigated. This plasmid was found to be segregationally and structurally hi

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31

Harris, Lyle Keenan. "Comparison of plasmids from clinical Lactobacillus strains." University of the Western Cape, 2018. http://hdl.handle.net/11394/6439.

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Magister Scientiae - MSc (Biotechnology)<br>The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has been found to be the predominant Lactobacillus species. Many studies have been conducted to assess factors influencing lactobacilli dominance in the vaginal microbiome. However, no study has evaluated the impact of plasmids on the vaginal lactobacilli. In the present study two plasmids, pLc17 and pLc4, isolated from vaginal Lactobacil

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32

MacLellan, Shawn R. Finan Turlough M. "Study of megaplasmid partitioning and replication initiation." *McMaster only, 2005.

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33

Gill, Santokh Singh Carleton University Dissertation Biology. "IncN group plasmids and plasmid regions determining the killing of Klebsiella pneumoniae and of immunity to killing." Ottawa, 1985.

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34

Williamson, Phillip C. "A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation." Thesis, University of North Texas, 2001. https://digital.library.unt.edu/ark:/67531/metadc2828/.

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Natural transformation is the process by which cells take up DNA from the surrounding medium under physiological conditions, altering the genotype in a heritable fashion. This occurs without chemical or physical treatment of the cells. Certain Acinetobacter strains exhibit a strong tendency to incorporate homologous DNA into their chromosomes by natural transformation. Transformation in Acinetobacter exhibits several unique properties that indicate this system's superiority as a model for transformation studies or studies which benefit from the use of transformation as an experimental method

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35

Beck, Kirsten. "Charakterisierung des Replikationsproteins ORF904 des archaealen Plasmids pRN1." kostenfrei, 2008. http://opus.ub.uni-bayreuth.de/volltexte/2009/545/.

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36

Magnusson, Terese. "Optimised plasmids for sustained transgene expression in vivo." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-123258.

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37

Meijer, Wilhelmus Johannes Jozef. "Replication and maintenance of plasmids in Bacillus subtilis." [S.l.] : [Groningen] : [s.n.] ; [University Library Groningen] [Host], 1995. http://irs.ub.rug.nl/ppn/141649046.

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38

Friis, Lorna M. "Campylobacter plasmids and their role in bacterial virulence." Thesis, University of East Anglia, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423504.

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39

Jackson, Robert Wilson. "Plasmids and virulence in Pseudomonas syringae pv. phaseolicola." Thesis, University of the West of England, Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389510.

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40

Owuama, C. I. "Genetic transformation of Saccharomyces cerevisiae with chimaeric plasmids." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381362.

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41

Park, Seonhee. "High-throughput intracellular delivery of proteins and plasmids." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54888.

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Intracellular delivery of macromolecules is crucial for the success of many research and clinical applications. Several conventional intracellular delivery methods have been used for many years but are still inadequate for several applications because of the issues associated with toxicity, low-throughput, and/or difficulty to target certain cell types. In this study, we developed and evaluated new high-throughput intracellular delivery methods for the efficient delivery of macromolecules while maintaining high cell viability. First, we studied the feasibility of using an array of nanoneedles,

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42

Comeaux, Jay Louis. "Transfer of plasmids by genetically-engineered Erwinia carotovora." Thesis, Virginia Tech, 1989. http://hdl.handle.net/10919/45933.

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<p>The ability of a genetically-engineered <i>Erwirzia carotovora</i> subsp. <i>carotovora</I> (Ecc) strain to transfer recombinant chromosomal DNA or plasmids to wildtype Ecc or <i>Pseudomonas fluorescens</i> was tested on filters, within soil microcosms, and <i>in planta</i>. Ecc was engineered by chromosomal insertion of a disarmed <i>endo</i>-pectate lyase gene marked with a 1.4kb DNA fragment conferring kanamycin resistance. Plasmids RPI and pBR322 were introduced separately into engineered Ecc clones. These strains served as donors in genetic transfer experiments. No transfer of the

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43

Zubrzycki, Igor Z. "Correlation between physical and genetic maps of plasmids." Doctoral thesis, University of Cape Town, 1992. http://hdl.handle.net/11427/21986.

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The aim of this study is to establish a correlation between the physical maps of plasmid DNA (in the form of calorimetric profiles, thermal denaturation profiles and electron micrographs of partly melted DNA sequences) and genetic maps of these DNAs and thus deal with questions which were not answered by previous researchers. viz: Is there a correlation between base sequence function and a measurable physical property which can be assigned to biologically important units such as promoters or coding sequences? Is there a correlation between the denaturation of gene sequences and cooperative tra

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44

Clark, Burton David. "Characterization of plasmids from Bacillus thuringiensis var. israelensis /." The Ohio State University, 1987. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487330761220416.

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45

Donaldson, Pauline A. (Pauline Alison) Carleton University Dissertation Biology. "Nodulation and tumorgenesis by Agrobacterium carrying Rhizobium plasmids." Ottawa, 1985.

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46

Poulter, Melinda D. "Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products." Thesis, University of North Texas, 1999. https://digital.library.unt.edu/ark:/67531/metadc2270/.

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TOL plasmids encode enzymes responsible for utilization of toluene and related aromatic compounds by Pseudomonas putida, ultimately converting them to central metabolic intermediates. The nucleotide sequence for the 5.6 kb xylGFJQK region of the pDK1 TOL meta operon was determined. DNA sequence analysis revealed the presence of five open reading frames corresponding to xylG (1458 bp), xylF (846 bp), xylJ (783 bp), xylQ (936 bp) and xylK (1047 bp), encoding predicted protein products of 51.6, 31.3, 27.8, 32.8, and 36.6 kDa in size, respectively. The average G+C content of the xylLTEGFJQK regi

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47

Hares, Douglas R. (Douglas Ryan). "Physical and Functional Characterization of the xy1XYZ Region From TOL Plasmid pDK1 and its Associated Downstream Regulatory Elements." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc278036/.

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The nucleotide sequence for the pDKl TOL plasmid region encoding toluate-1,2-dioxygenase (Xy1XYZ, TO) was determined. TO is the first enzyme in the meta-cleavage operon, responsible for the conversion of toluates and benzoates to their carboxy-substituted diols. DNA sequence analysis revealed the presence of three open reading frames (ORF). The three ORFs correspond to xylX (1353 bp), xylY (486 bp) and xylZ (1008 bp), encoding predicted protein products of 51370 Da, 19368 Da and 36256 Da, respectively.

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48

Aylett, Christopher Herbert Stanley. "A structural study of the TubZRC plasmid partitioning system." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609711.

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49

Van, Zyl Leonardo Joaquim. "Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/70109.

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Thesis (MSc)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: The 14.2 kb plasmid pTC-FI4 was isolated from the moderately thermophilic (45°- 50°C), highly acidophilic (pH 1.5 to 2.5), chemolithotrophic bacterium Acidithiobacil/us caldus and has a replicon that is closely related to the promiscuous, broad host-range, IncQ-family of plasmids. The region containing the mobilization genes was sequenced and encoded five Mob proteins and an origin of transfer, which are related to the DNA processing (Tral) region of IncPI plasmids, rather than to the three Mob protein systems of the I

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50

Meakin, Stephanie Asalyn Carleton University Dissertation Biology. "The molecular biology of methanogens: cell lysis, plasmid survey and the characterization of a novel plasmid." Ottawa, 1992.

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