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Teses / dissertações sobre o tema "Pseudomonas fluorescens"

Autor: Grafiati
Publicado: 4 de junho de 2021
Última modificação: 25 de julho de 2025

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1

Blankemeier, Andrew R. "Characterization of Pseudomonas fluorescens Biofilm." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1307731184.

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2

Hamel, Robert D. "Aluminum detoxification mechanisms in Pseudomonas fluorescens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0025/MQ31433.pdf.

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3

Clements, Richard Steven. "The serological homology of Pseudomonas fluorescens proteases." Thesis, Queensland University of Technology, 1987. https://eprints.qut.edu.au/36720/1/36720_Clements_1987.pdf.

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This investigation evaluated the serological homology of proteases from a number of psychotrophic bacteria consisting largely of Pseudomonas_fluorescens strains. Proteases from 4 reference strains of P.fluorescens were purified and rabbit antiserum generated against each. Preliminary investigations revealed the serological heterology of protease OM82 to proteases N73A, M143A and OMl 86. A total of 54, presumably P.fluorescens strains, were grown and semi-pure protease preparations obtained from each. The immunological cross reactivity of 54 semi-pure protease preparations with each
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4

Wang, Chien-Sao. "Cell-Free Recovery and Isotopic Identification of Cyanide Degrading Enzymes from Pseudomonas Fluorescens." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc278363/.

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Cell-free extracts from Pseudomonas fluorescens NCIMB 11764 catalyzed the degradation of cyanide into products that included C02, formic acid, formamide and ammonia. Cyanide-degrading activity was localized to cytosolic cell fractions and was observed at substrate concentrations as high as 100 mM. Two cyanide degrading activities were identified by: (i) the determination of reaction products stoichiometries, (ii) requirements for NADH and oxygen, and (iii) kinetic analysis. The first activity produced CO2 and NH3 as reaction products, was dependent on oxygen and NADH for activity, and displaye
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5

Levasseur, Rémi. "Aluminum citrate transport and metabolism in Pseudomonas fluorescens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0018/MQ46489.pdf.

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6

Wan, Dagang Wan Rosmiza Zana. "Understanding adhesion of Pseudomonas fluorescens on household surfaces." Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/3821/.

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In this study, three different methods have been used to investigate the bacterial interaction with the substratum, i.e. atomic force microscopy (AFM), spinning disc and micromanipulation. Pseudomonas fluorescens NCIMB 9046 was chosen as a model microorganism to study the cell-substrate adhesion. By having three different colloidal particles: stainless steel (Grade 304), glass and cellulose, the force measurements were performed in growth medium and ambient air using AFM. The results demonstrated that the adhesive forces were influenced by the surface hydrophobicity, electrostatic, van der Waa
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7

Macioszek, Malgorzata. "Biosynthesis of mupirocin by Pseudomonas fluorescens NCIMB 10586." Thesis, University of Birmingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.510237.

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Mupirocin, a polyketide antibiotic active against Gram-positive bacteria is used clinically for treatment of bacterial skin infections, to clear Stapylococcus aureus ftom nasal passages and as a surgical scrub to inhibit bacterial growth, particularly that of MRSA. Mupirocin is synthesised by polyketide synthases (PKS) in a series of reactions involving many enzymes encoded by genes from the mupirocin cluster. The mupirocin cluster consists of six larger ORFs (mmpA-F. ) encoding multifunctional proteins, and twenty nine individual genes (mupA-X and macpA-E) all of which have been shown to be r
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8

Frey-Klett, Pascale. "Ecologie d'un pseudomonas fluorescens auxiliaire de la mycorhization." Paris 11, 1996. http://www.theses.fr/1996PA112480.

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La souche de bacterie auxiliaire de la mycorhization pseudomonas fluorescens bbc6, isolee d'un carpophore de laccaria laccata, stimule l'etablissement de la symbiose entre le douglas et ce champignon ectomycorhizien. Dans le but de comprendre son mode d'action mais aussi pour integrer de facon raisonnee son inoculation aux programmes de mycorhization controlee du douglas, nous avons etudie l'ecologie de la souche bbc6 en serre et en pepiniere. Nous avons compare les caracteristiques phenotypiques et genotypiques de bbc6 a celles de 300 souches de pseudomonas fluorescents isolees du sol nu, de
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9

Koza, Anna. "Adaptation and niche construction by Pseudomonas fluorescens SBW25." Thesis, Abertay University, 2011. https://rke.abertay.ac.uk/en/studentTheses/7888a5ac-f562-4518-8124-36d2e394994d.

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The mechanisms underlying adaptive radiation or evolution have been extensively investigated using experimental bacterial populations in liquid cultures, referred to as microcosms. Evolving populations of <i>Pseudomonas fluorescens</i> SBW25 in static microcosms reproducibly lead to the emergence of the Wrinkly Spreader (WS) genotype. These produce a cellulose-matrix-based biofilm to colonise the airliquid (A-L) interface with significant fitness advantage over non-biofilm-forming competitors. In this work, the first SBW25 colonists in static microcosms were shown to establish O<sub>2</sub> gr
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10

Kulkarni, N. "Studies on lipase enzyme from pseudomonas fluorescens NS2W." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2002. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2333.

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11

Delafuente, Leonardo. "Characterization of the ecological and physiological basis of superior rhizosphere colonization by 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas genotypes." Online access for everyone, 2005. http://www.dissertations.wsu.edu/Dissertations/Fall2005/l%5Fdelafuente%5F1082405.pdf.

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12

Uría, Fernández Diana. "Strukturaufklärung der Siderophore und massenspektrometrische Untersuchung eines ihrer Rezeptorproteine von Pseudomonas fluorescens G173." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964735342.

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13

Janzen, Elena. "Die Benzaldehydlyase aus Pseudomonas fluoreszens biochemische Charakterisierung und die Untersuchung von Struktur-Funktionsbeziehungen /." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=967208629.

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14

Jackson, Benjamin. "Molecular genetics of #alpha# pinene metabolism in Pseudomonas fluorescens." Thesis, Imperial College London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343598.

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15

Scott, Robert W. "Synthetic strategies to metabolites from mutants of pseudomonas fluorescens." Thesis, University of Bristol, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558095.

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Mupirocins F and H are metabolites isolated from cultures of mutant strains of Pseudomonas f1uorescens. Synthetic routes have been developed to these compounds to confirm their structures Investigations into the synthesis of 22 led to two novel rearrangement products 48 and 55 and their structures were confirmed through extensive spectroscopic studies. Methyl mupirocin F was prepared in two steps from pseudomonic acid A via methylation with TMS- diazomethane to give methyl pseudomonate 3 followed by a selective TEMPO oxidation to yield methyl mupirocin F 22. The total synthesis of mupirocin H
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16

Griffiths, M. B. "Genetic analysis of ecgonine degradation by Pseudomonas fluorescens (MBER)." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599726.

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This study details a genetic investigation of ecgonine degradation by <I>P. fluorescens</I> MBER. Transposon (Tn5) insertional inactivation mutagenesis was performed to create mutants blocked in the ecgonine degradative pathway. By this method, 87 such mutants were isolated and analysed to identify where in the genome the interruption had occurred. At the whole cell and cell-free extract level, no intermediary metabolites were detected in these mutants by either TLC or GC/MS analysis. The genetic location of Tn5 in the DNA was therefore investigated to obtain sequence data around the insertion
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17

Reid, H. D. "An investigation of Pseudomonas fluorescens in the milk environment." Thesis, University of Canterbury. Microbiology, 1997. http://hdl.handle.net/10092/6873.

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The main aim of this study was to determine the percentage of Pseudomonas fluorescens amongst fluorescent pseudomonads in the milk environment, and to distinguish these isolates from each other. A secondary aim was to investigate whether these isolates were clonal or non-clonal in origin, and to study whether end product contamination had an endemic factory source. In this study 230 fluorescent bacteria were isolated from a South Island dairy company over a six month period. These bacteria were isolated from raw milk and associated environments, separated milk and associated environments, pas
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18

McCloskey, Nicholas. "Transcriptional Effects of Adaptive Synonymous Mutations in Pseudomonas fluorescens." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/37903.

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Synonymous mutations have traditionally been thought to have no significant effect on fitness. However, a growing body of recent research has shown that this is not always the case. In an experimentally evolved population of Pseudomonas fluorescens grown in minimal glucose media, synonymous mutations arose in a glucose transport gene that resulted in beneficial fitness effects comparable to those of non-synonymous mutations. We found that the increase in fitness was a direct result of increased gene expression; however, the precise mechanism was unclear. Synonymous mutations have been shown to
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19

Nagappan, Olagappan. "Mechanisms of Cyanide Assimilation in Pseudomonas fluorescens NCIMB 11764." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc278533/.

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Pseudomonas fluorescens NCIMB 11764 was capable of utilizing cyanide as a sole nitrogen source for growth. Cyanate (OCN") and S-cyanoalanine could also serve as nitrogenous substrates, but do not appear to play a role as intermediates in cyanide metabolism. Growth of this strain on cyanate as the sole nitrogen source led to the induction of an enzyme characterized as a cyanase (EC 3.5.5.3) based on its stoichiometric conversion of cyanate to ammonia, and dependence on bicarbonate for maximal activity. However, since cyanase activity was not elevated in cyanide-grown cells it was concluded that
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20

Lin, Hao. "PREDICTION OF GROWTH OF PSEUDOMONAS FLUORESCENS UNDER TEMPERATURE FLUCTUATION." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429840331.

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21

Senatore, Marcela Andrea Duran Haun 1974. "Avaliação da eficiencia de um esterilizador a plasma na inativação de Pseudomonas fluorescens." [s.n.], 2004. http://repositorio.unicamp.br/jspui/handle/REPOSIP/254838.

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Orientador: Marcelo Cristianini<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos<br>Made available in DSpace on 2018-08-03T20:56:17Z (GMT). No. of bitstreams: 1 Senatore_MarcelaAndreaDuranHaun_M.pdf: 684290 bytes, checksum: 522970fe79261c7d2890c0a19a39b587 (MD5) Previous issue date: 2004<br>Resumo: Um dos problemas que enfrenta a indústria de laticínios é a recontaminação do leite decorrente da formação de biofilmes em tanques de armazenamento e trocadores de calor. A tecnologia de esterilização de materiais por gás plasma tem sido utilizada
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22

Barrett, Rowan Douglas Hilton. "Experimental evolution of Pseudomonas fluorescens in simple and complex environments." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97902.

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Determining the factors responsible for the origin and maintenance of diversity remains a difficult problem in evolutionary biology. There is extensive theoretical work which suggests that environmental heterogeneity plays a major role. This theory argues that diversification is ultimately due to divergent natural selection for alternative resources. In this thesis I investigate adaptation and the evolution of diversity in experimental populations of the asexual bacterium Pseudomonas fluorescens. In all experiments I introduce clonal isolates of Pseudomonas to a novel environment and allow evo
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23

Turnbull, Gillian Anne. "The role of motility in Pseudomonas fluorescens and Pseudomonas putida in soil-plant-microbe interactions." Thesis, University of Liverpool, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367105.

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24

Kondakova, Tatiana. "Pseudomonas adaptation to stress factors : role of membrane lipids and Pseudomonas fluorescens response to NO2." Rouen, 2015. http://www.theses.fr/2015ROUES016.

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La large distribution des Pseudomonas fluorescens est liée à leur grande adaptabilité aux facteurs de stress tels que les variations environnementales. Ce travail avait pour objet la réponse spécifique au milieu aéroporté de P. Fluorescens, comme l’aéroportée MFAF76a et la clinique MFN1032 comme standard. La technique récente HPTLC-MALDI-TOF MSI a permis de caractériser les divers glycérophospholipides (GP) des deux souches. En phase stationnaire de croissance, un GP inconnu (UGP - unknown GP) a été isolé et semble intervenir dans l’adaptation à la température de la souche clinique MFN1032. Qu
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25

Le, Clanche Jean-François. "La petite agriciculture : survivance du passé ou agriculture en devenir ? : Une approche bioéconomique." Rennes, Agrocampus Ouest, 2013. http://www.theses.fr/2013NSARE035.

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Petites exploitations, petits exploitants: ces mots éveillent en nous l’image d’une paysannerie aujourd’hui disparue, d’une agriculture condamnée par la modernité. D’autres esprits s’enflamment et y voient, à contrario, une issue probable pour dépasser les failles actuelles du modèle de développement productiviste. Si le nombre de petites exploitations a chuté vertigineusement depuis plus d’un siècle, elles représentent encore entre 20 et 25% du nombre total d’exploitations. Les économistes prévoyaient leur disparition. Leur prévision s’est même faite certitude jusqu’à s’imposer comme étant l’
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26

Lou, Chun-hin. "Multilocus sequence typing of pseudomonas fluorescens isolates from investigation of a case of transfusion-associated sepsis." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42925320.

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27

Leneveu-Jenvrin, Charlène. "Virulence et adaptation à l'environnement de souches de Pseudomonas fluorescens et Pseudomonas mosselii ; contribution à l'étude des conditions d'expression et des fonctions de la protéine TSPO chez P. Fluorescens." Rouen, 2014. http://www.theses.fr/2014ROUES033.

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Ce mémoire est dédié d'une part à l'étude de la virulence de Pseudomonas mosselii, et d'autre part à l'étude du rôle de la protéine TSPO chez Pseudomonas fluorescens Pf0-1. P. Mosselii est une bactérie isolée du milieu hospitalier qui a été caractérisée en 2002 mais dont le pouvoir pathogène reste inconnu à ce jour. Nous avons pu observer que P. Mosselii ATCC BAA-99 et P. Mosselii MFY161 sont cytotoxiques, peu invasives mais capables d'altérer la perméabilité épithéliale des cellules Caco-2/TC7 et d'endommager le cytosquelette d'actine. Leur comportement ressemble à celui de certaines souches
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28

Shen, Weiping. "Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc278188/.

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Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response to exogenously fed pyrimidine compounds. Thus, it was possible to bring about changes in pyrimidine
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29

Teselkin, Oleksiy. "Repeatability of the Adaptation of Pseudomonas fluorescens to Low Glucose." Thèse, Université d'Ottawa / University of Ottawa, 2014. http://hdl.handle.net/10393/30986.

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Inspired by Gould, who claimed life would be arriving at a different outcome each time it were allowed to run from the same beginning, I have attempted to determine the repeatability of the adaptive course of one Pseudomonas fluorescens lineage. In addition, my study aimed to establish whether the likelihood of parallel evolution of the two synonymous single-nucleotide substitutions was contingent upon a prior motility-impairing deletion or a prior increase in fitness. Further, the study was designed to provide empirical data addressing the long-standing question of the effect of starting fitn
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30

Dorr, Patrick Karl. "Cyanide oxygenase and cyanase activities of Pseudomonas fluorescens NCIMB 11764." Thesis, University of Kent, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292714.

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31

Braithwaite, Kerynne Lindsay. "Novel plant cell wall hydrolases from Pseudomonas fluorescens subspecies cellulosa." Thesis, University of Newcastle Upon Tyne, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294928.

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32

Gurney, Rachel. "Biosynthesis of the antibiotic mupirocin by Pseudomonas fluorescens NCIMB 10586." Thesis, University of Birmingham, 2013. http://etheses.bham.ac.uk//id/eprint/4204/.

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The mupirocin biosynthetic pathway belongs to the trans-AT group in which acyltransferase (AT) activity is provided by a separate polypeptide (MmpC) rather than in cis as found in the typical type I polyketide synthases. AT docking domains have been documented in trans-AT PKS clusters for ten years yet little functional evidence is available. The cluster shows many interesting features that must be understood to create novel products. Specificity studies demonstrated that AT2 performs the typical AT function of loading malonyl-CoA to ACPs throughout the cluster. Mutagenesis studies demonstrate
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33

Amin-Hanjani, Soheila. "Luminescence based detection of genetically modified Pseudomonas fluorescens in soil." Thesis, University of Aberdeen, 1992. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU043327.

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Methods currently available for the detection and enumeration of genetically modified micro-organisms in the environment include culturing methods, direct microscopic detection and nucleic hybridization techniques. The aim of this project was to develop luminescence as a molecular based-marker system in Pseudomonas fluorescens. The lux genes, originally isolated from Vibrio fischeri, were introduced into Ps. fluorescens on plasmid vectors and on the chromosome. The efficiency of these two strategies for the detection of Ps. fluorescens in soil was assessed. Luminometry was used to estimate bio
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34

Chou, Chia-Ni. "Purification of Cyanide-Degrading Nitrilase from Pseudomonas Fluorescens NCIMB 11764." Thesis, University of North Texas, 2010. https://digital.library.unt.edu/ark:/67531/metadc33224/.

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Cyanide is a well known toxicant that arises in the environment from both biological and industrial sources. Bacteria have evolved novel coping mechanisms for cyanide and function as principal agents in the biosphere for cyanide recycling. Some bacteria exhibit the unusual ability of growing on cyanide as the sole nitrogen source. One such organism is Pseudomonas fluorescens NCIMB 11764 (Pf11764) which employs a novel oxidative mechanism for detoxifying and assimilating cyanide. A unique complex of enzymes referred to as cyanide oxygenase (CNO) is responsible for this ability converting cyanid
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35

Dal, Bello Elena <1983&gt. "Vanillin production from ferulic acid with Pseudomonas fluorescens BF13-1p4." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amsdottorato.unibo.it/5482/1/PhD_Thesis_EDB.pdf.

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Bioconversion of ferulic acid to vanillin represents an attractive opportunity for replacing synthetic vanillin with a bio-based product, that can be label “natural”, according to current food regulations. Ferulic acid is an abundant phenolic compound in cereals processing by-products, such as wheat bran, where it is linked to the cell wall constituents. In this work, the possibility of producing vanillin from ferulic acid released enzymatically from wheat bran was investigated by using resting cells of Pseudomonas fluorescens strain BF13-1p4 carrying an insertional inactivation of vdh gene an
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36

Dal, Bello Elena <1983&gt. "Vanillin production from ferulic acid with Pseudomonas fluorescens BF13-1p4." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amsdottorato.unibo.it/5482/.

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Bioconversion of ferulic acid to vanillin represents an attractive opportunity for replacing synthetic vanillin with a bio-based product, that can be label “natural”, according to current food regulations. Ferulic acid is an abundant phenolic compound in cereals processing by-products, such as wheat bran, where it is linked to the cell wall constituents. In this work, the possibility of producing vanillin from ferulic acid released enzymatically from wheat bran was investigated by using resting cells of Pseudomonas fluorescens strain BF13-1p4 carrying an insertional inactivation of vdh gene an
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37

Fields, Christopher J. "Comparative biochemistry and genetic analysis of nucleoside hydrolase in Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas fluorescens." Thesis, University of North Texas, 2002. https://digital.library.unt.edu/ark:/67531/metadc3290/.

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The pyrimidine salvage enzyme, nucleoside hydrolase, is catalyzes the irreversible hydrolysis of nucleosides into the free nucleic acid base and D-ribose. Nucleoside hydrolases have varying degrees of specificity towards purine and pyrimidine nucleosides. In E. coli, three genes were found that encode homologues of several known nucleoside hydrolases in protozoa. All three genes (designated yaaF, yeiK, and ybeK) were amplified by PCR and cloned. Two of the gene products (yeiK and ybeK) encode pyrimidine-specific nucleoside hydrolases, while the third (yaaF) encodes a nonspecific nucleoside hy
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38

Selsaas, Eirik. "Karakterisering av gener som påvirker biosyntesen av alginat i Pseudomonas fluorescens." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-12753.

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Pseudomonas fluorescens har evne til å produsere store store mengder alginat. Tidligere er det laget en stamme, P. fluorescens SBW25 MS1 &#916;algC::TnKb61, hvor algD-operonet og algC er satt under kontroll av Pm-promotor. Dette gjør at den produserer alginat ved tilsats av m-toluensyre. Denne stammmen har blitt mutert med transposon og effekten på alginatproduksjonen har blitt undersøkt. Ved transposonmutagenese ble det funnet 180 mutanter med endret alginatproduksjon, og insersjonspunktet for transposonet ble identifisert.I to av disse mutantene var henholdsvis genene clpA og PFLU3927 inakti
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39

Burlinson, Peter. "Characterization and regulation of anti-eukaryote factors in Pseudomonas fluorescens. NZI7." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491327.

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This study examined Pseudomonas 'NZ strains' that cause blotch disease on the commercial mushroom Agaricus bisporus. Mushroom-pathogenic Pseudomonas represent an interesting example of bacteria associated with a fungal host system. The mycosphere also contains other organisms that Pseudomonas may interact with, thus strains isolated from this environment may also contribute towards our understanding of the molecular basis of opportunistic pathogenesis and bacterial survival in nature. Phylogenetics was used to assess the relatedness of NZ strains and their position in the Pseudomonas genus. It
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40

Melnyk, Anita H. "Adaptive landscapes in evolving populations of Pseudomonas fluorescens in simple environments." Thesis, University of Ottawa (Canada), 2010. http://hdl.handle.net/10393/28876.

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The adaptive landscape heuristic can be used to answer the question "how predictable is evolution?" because its topology will impact the repeatability of evolution. In my Masters research I addressed this question in two ways: (1) I reviewed empirical adaptive landscape studies in the fields of directed protein evolution and microbial experimental evolution and (2) I performed a selection experiment to characterize adaptive landscape topology by measuring variance in fitness and metabolic phenotype within and among genetically distinct Pseudomonas fluorescens strains in two environments. Empir
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41

McCarthy, Conor Neil, and n/a. "Regulatory Elements Controlling Lipase and Metalloprotease Production in Pseudomonas fluorescens B52." Griffith University. School of Health Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20031015.124744.

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Psychrotrophic bacteria, such as Pseudomonas fluorescens B52, are a major cause of milk spoilage at refrigeration temperature due to the production of lipolytic and proteolytic enzymes. Regulatory mechanisms controlling the production of lipase and protease by the B52 lipA and aprX genes were investigated. Transposon mutagenesis identified the possible involvement of a poly-A polymerase enzyme which destabilises mRNA by 3' polyadenylation. A homologue of the E. coli EnvZ/OmpR two-component sensor/regulator system was identified by transposon mutagenesis and shown to repress lipase and protease
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42

Shields, Jennifer Ann. "Biosynthesis of the polyketide antibiotic mupirocin by Pseudomonas fluorescens NCIMB 10586." Thesis, University of Birmingham, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506425.

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A 75 kb gene cluster in Pseudomonasfl uorescens NCIMB 10586 encodesa modular polyketide synthase responsible for biosynthesis of the antibiotic mupirocin. All seven PKS modules lack intrinsic acyl transferase (AT) domains. Two AT domains within the multifunctional polypeptide MmpC are thought to operate in trans. An unusually large tailoring region encodes 26 discrete proteins that modify the polyketide/fatty acid backbone, in addition to providing resistance and regulatory functions. This study investigated the functions of four tailoring region genes. MupJ, MupK and mAcpC were revealed to be
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43

Ferreira, Luis Manuel dos Anjos. "Molecular analysis of cellulases and xylanses from Pseudomonas fluorescens subspecies cellulosa." Thesis, University of Newcastle Upon Tyne, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316083.

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44

Kellett, Louise Elizabeth. "The molecular biology of xylanolytic enzymes from Pseudomonas fluorescens subsp. cellulosa." Thesis, University of Newcastle Upon Tyne, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315636.

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45

Zahran, Ahmed Shawky. "Production and properties of a protease secreted by Pseudomonas fluorescens R8." Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/11965.

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46

Wilkins, Annekathrin. "Factors influencing the dispersal of Pseudomonas fluorescens NZI7 by Caenorhabditis elegans." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:6bf58183-f197-490d-86d4-633ae8d46c06.

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Caenorhabditis elegans is a natural predator of the mushroom pathogen Pseudomonas fluorescens NZI7. The bacterial mechanisms for reducing predation by the nematode through the secretion of secondary metabolites have been described, but not yet fully explored. The behaviour of nematodes is influenced by the different factors produced by the pseudomonads. In this thesis we develop a range of assays to link the behaviour of C. elegans to these factors to identify their role in bacteria-nematode interactions. We show that these factors play two distinct roles: they may either repel nematodes, or h
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47

Parab, Preeti. "Requirements for Cell-Free Cyanide Oxidation by Pseudomonas Fluorescens NCIMB 11764." Thesis, University of North Texas, 2000. https://digital.library.unt.edu/ark:/67531/metadc2614/.

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The involvement of cyanide oxygenase in the metabolism of pyruvate and a-ketoglutarate-cyanohydrin was investigated and shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. Thus, free cyanide remains the substrate, for which the enzyme displays a remarkably high affinity (Kmapp,4 mM). A model for cyanide utilization is therefore envisioned in which the substrate is initially detoxified by complexation to an appropriate ligand followed by enzymatic oxidation of cyanide arising at sublethal levels via chemical dissociation.
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48

McCarthy, Conor Neil. "Regulatory Elements Controlling Lipase and Metalloprotein Production in Pseudomonas fluorescens B52." Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367432.

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Psychrotrophic bacteria, such as Pseudomonas fluorescens B52, are a major cause of milk spoilage at refrigeration temperature due to the production of lipolytic and proteolytic enzymes. Regulatory mechanisms controlling the production of lipase and protease by the B52 lipA and aprX genes were investigated. Transposon mutagenesis identified the possible involvement of a poly-A polymerase enzyme which destabilises mRNA by 3' polyadenylation. A homologue of the E. coli EnvZ/OmpR two-component sensor/regulator system was identified by transposon mutagenesis and shown to repress lipase and protease
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49

Careli, Roberta Torres. "Adesão de Pseudomonas fluorescens em superfícies utilizadas no processamento de alimentos." Universidade Federal de Viçosa, 2005. http://www.locus.ufv.br/handle/123456789/9091.

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Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2016-11-08T17:50:12Z No. of bitstreams: 1 texto completo.pdf: 848123 bytes, checksum: 39ad4634d41468604f2afeb499a0ef04 (MD5)<br>Made available in DSpace on 2016-11-08T17:50:12Z (GMT). No. of bitstreams: 1 texto completo.pdf: 848123 bytes, checksum: 39ad4634d41468604f2afeb499a0ef04 (MD5) Previous issue date: 2005-07-27<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>A adesão de Pseudomonas fluorescens ATCC 13525 foi avaliada pela microscopia de epifluorescência (EPF) e contagem padrão em placas (CPP
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50

Andreani, Nadia Andrea. "INTO THE BLUE: Spoilage phenotypes of Pseudomonas fluorescens in food matrices." Doctoral thesis, Università degli studi di Padova, 2016. http://hdl.handle.net/11577/3424342.

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Spoilage induced by Pseudomonas strains is commonly found in a wide range of food products as a result of the ubiquitous presence of these strains and their ability to induce alteration through different mechanisms. Particular attention has been recently paid on those P. fluorescens strains able to induce a blue discolouration on several food matrices (e.g. dairy or meat products). Actually, poor data are available about this curious event that draw the attention of European consumer from 2010. In the present manuscript a step-by-step investigation of the spoilage potential of Pseudomonas fl
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