Bibliografias temáticas / Psychosocial Enzyme-linked immunosorbent assay (ELISA)

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Literatura científica selecionada sobre o tema "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Autor: Grafiati

Publicado: 15 de setembro de 2021

Última modificação: 30 de julho de 2025

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Artigos de revistas sobre o assunto "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Guo, Huirong, Yuming Ren, Bailing Huang, Junru Wang, Xuhuang Yang, and Yali Wang. "Psychological Status, Compliance, Serum Brain-Derived Neurotrophic Factor, and Nerve Growth Factor Levels of Patients with Depression after Augmented Mindfulness-Based Cognitive Therapy." Genetics Research 2022 (January 4, 2022): 1–5. http://dx.doi.org/10.1155/2022/1097982.

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Objective. Mindfulness-based cognitive therapy (MBCT) is a cost-effective psychosocial program that prevents relapse/recurrence in major depression. The present study aimed to analyze the effects of augmented MBCT along with standard treatment dominated by pharmacotherapy on psychological state, compliance, brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) expression levels in patients with depression. Methods. A total of 160 eligible patients with depression in The First Affiliated Hospital of Zhengzhou University were included in this study. The study randomly assigned

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Monteleone, P., P. Scognamiglio, B. Canestrelli, I. Serino, A. M. Monteleone та M. Maj. "Asymmetry of salivary cortisol and α-amylase responses to psychosocial stress in anorexia nervosa but not in bulimia nervosa". Psychological Medicine 41, № 9 (2011): 1963–69. http://dx.doi.org/10.1017/s0033291711000092.

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BackgroundThe stress response involves the activation of the hypothalamic–pituitary–adrenal (HPA) axis and the sympathetic nervous system (SNS). As a role for stress in determining of the onset and the natural course of eating disorders (EDs) has been proposed, the study of the psychobiology of the stress response in patients with anorexia nervosa (AN) and bulimia nervosa (BN) should be helpful in understanding the pathophysiology of these disorders. The two neurobiological components of the stress response can be easily explored in humans by the measurement of salivary cortisol and α-amylase

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LI, Bei, Xiao-hong DUAN, Jin-feng WU, et al. "Impact of psychosocial stress on airway inflammation and its mechanism in a murine model of allergic asthma." Chinese Medical Journal 126, no. 2 (2013): 325–34. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20120685.

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Background It has already been recognized that psychosocial stress evokes asthma exacerbation; however, the mechanism of how stress gets inside the body is not clear. This study aimed to observe the impact of psychosocial stress on airway inflammation and its mechanism in the ovalbumin-induced asthmatic mice combined with social disruption stress. Methods Thirty-six male BALB/c mice were randomly divided into: control group, asthma group (ovalbumin-induced), asthma plus social disruption stress group (SDR), and SDR group. The open field video tracking system was used to assess animal behaviors

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Ningrum, Aji Mustika, Prananda Surya Airlangga, Wiwiek Indriyani Maskoep, and Herdiani Sulistyo Putri. "Relationship of quinolinic acid and serotonin with depression and pain degree in cancer pain patients: a cross-sectional study." Bali Medical Journal 12, no. 3 (2023): 2681–84. http://dx.doi.org/10.15562/bmj.v12i3.4697.

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Link of Video Abstract: https://youtu.be/zg5vubHMcDY Introduction: Chronic pain requires a thorough assessment if it persists in patients with cancer. Depressive disorder is one of the psychosocial problems that can occur in cancer patients. Depressive disorders affect up to 34.4% of patients with cancer in Indonesia, causing increased morbidity and complicating the management of patients. Quinolinic acid (QUIN) and serotonin (5-HT) are chemicals that can affect pain perception and depressive disorders. This study aims to analyze the relationship between quinolinic acid and serotonin levels wi

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Dita, Maya Chandra. "Enzyme Linked Immunosorbent Assay (ELISA)." Natural Sciences Engineering and Technology Journal 1, no. 2 (2021): 29–38. http://dx.doi.org/10.37275/nasetjournal.v1i2.6.

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Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in many industries. ELISA has been the system of choice when testing soluble antigens and antibodies. EIA / ELISA uses the basic immunological concept of antigen binding to specific antibodies, which allows the detection of small amounts of antigens such as proteins, peptides, hormones or antibodies in fluid samples. In all protocols, solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled with the enzyme.

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Engvall, Eva. "The ELISA, Enzyme-Linked Immunosorbent Assay." Clinical Chemistry 56, no. 2 (2010): 319–20. http://dx.doi.org/10.1373/clinchem.2009.127803.

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Lequin, Rudolf M. "Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)." Clinical Chemistry 51, no. 12 (2005): 2415–18. http://dx.doi.org/10.1373/clinchem.2005.051532.

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Abstract This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient sampl

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Hidayat, Rachmat, and Patricia Wulandari. "Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 2 (2021): 352–58. http://dx.doi.org/10.32539/bsm.v5i2.228.

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A B S T R A C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle of antigen-antibody binding. In the ELISA technique, antigenimmobilization will be carried out on a solid surface, then bound withantibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the formof a color change will be formed due to the reaction between the enzyme andthe substrate.

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Hidayat, Rachmat, and Patricia Wulandari. "Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 5 (2021): 447–53. http://dx.doi.org/10.32539/bsm.v5i5.228.

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ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

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Kohl, Thomas O., and Carl A. Ascoli. "Direct Competitive Enzyme-Linked Immunosorbent Assay (ELISA)." Cold Spring Harbor Protocols 2017, no. 7 (2017): pdb.prot093740. http://dx.doi.org/10.1101/pdb.prot093740.

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Teses / dissertações sobre o assunto "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Nicholas, Lionel John. "The development of a university-based sex counselling programme in the age of AIDS." University of the Western Cape, 1993. http://hdl.handle.net/11394/8447.

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Philosophiae Doctor - PhD<br>The sexual behaviours, attitudes, beliefs and communication of 1896 black first-year university students were examined by means of a structured questionnaire for their contribution to the development of a university-based sex counselling programme. The areas of sexuality investigated included intra-familial communication about contraception and sexuality, belief in sex myths, knowledge of and myths about AIDS and the manner of acquisition of sex knowledge. The results of this study are consistent in reflecting much greater deficits in the knowledge of respondents

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Kim, In Soo. "A quantitative enzyme linked immunosorbent assay for polychlorinated biphenyls in transformer oil." Thesis, Cranfield University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323838.

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Leung, Sau-man Sally. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2337309X.

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梁秀雯 and Sau-man Sally Leung. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970096.

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Lupica, Samuel J. "Nitrate Toxicity to Common Carp Measured Noninvasively by Novel Enzyme-linked Immunosorbent Assay for Cortisol." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1226956326.

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Sheehan, C. P. "The application of the enzyme-linked immunosorbent assay (ELISA) to ABH grouping in forensic science." Thesis, University of Surrey, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381661.

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Cheow, Lih Feng. "Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/55148.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 66-68).<br>Experimental studies were performed to evaluate the kinetics and equilibrium binding constants of biomolecules in nanofluidic channels. Binding events in the nanochannel were detected using electrical and fluorescence methods. We concluded that antibody-antigen binding constants in nanochannels were similar to experiments performed in microtiter plates at low antigen concentrations; howev

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Johnson, Raymond Camille Joseph. "Detection of nepoviruses by ELISA in tissue-cultured and field-grown grapevines." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27968.

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The detection by serology of nematode-transmitted polyhedral viruses (nepoviruses) in grapevines is often unreliable. Nepoviruses were detected by enzyme-linked immunosorbent assay (ELISA) in tissue-cultured and field-grown grapevines. Nepovirus detection in in vitro plants (plantlets) was affected by virus distribution and growth room temperature. The reliability of virus detection in field-grown grapevines was improved when modified grinding buffers were used. Arabis mosaic virus (AMV) was detected by ELISA, for the first time, in in vitro grapevines initiated from field-and screenhouse-gro

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Orchard, Robert Graham. "A flow-through enzyme-linked immunoassay for progesterone." The University of Waikato, 2007. http://hdl.handle.net/10289/2277.

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Bovine reproductive performance is one of the most important factors influencing dairy farm profitability. Present-day techniques for oestrus- and pregnancy-detection are unreliable and labour-intensive. Although measuring milk-progesterone at regular intervals allows the fertility status of a cow to be determined reliably, the labour cost of collecting and analysing samples is prohibitive. This project aimed to develop a progesterone sensing system that could be automated and integrated with the milking unit, thus minimising labour costs. The proposed system involved mixing the milk sample wi

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Hitt, John Michael 1952. "DETERMINATION OF THE EFFICACY OF THE ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) IN CHARACTERIZING CROTALUS SNAKE VENOM AT THE SPECIES LEVEL." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/276352.

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Livros sobre o assunto "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Hosseini, Samira, Patricia Vázquez-Villegas, Marco Rito-Palomares, and Sergio O. Martinez-Chapa. Enzyme-linked Immunosorbent Assay (ELISA). Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-6766-2.

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M, Kemeny D., and Challacombe Stephen J, eds. ELISA and other solid phase immunoassays: Theoretical and practical aspects. 1988., 1988.

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United States. Environmental Protection Agency. Office of Research and Development, Battelle Memorial Institute, and ETV Advanced Monitoring Systems Center (U.S.), eds. Tetracore, Inc: Anthrax, Botulinum toxin, and Ricin Enzyme-Linked Immunosorbent Assay (ELISA). U.S. Environmental Protection Agency, Office of Research and Development, 2004.

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W, Burgess Graham, and James Cook University of North Queensland. Graduate School of Tropical Veterinary Sciences., eds. ELISA technology in diagnosis and research. Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, 1988.

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Darcel, Colin. A collection of essays on comparative medicine, and a bibliography of references to the ELISA test. Palliser Animal Health Laboratories, 1996.

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Daniel, Carlota Leonor. "Enzyme-Linked Immunosorbent Assay" (ELISA) zum Nachweis von Babesia bovis- und Anaplasma marginale- Infektionen in Argentinien. [s.n.], 1993.

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United States. Environmental Protection Agency. Office of Research and Development, Battelle Memorial Institute, and ETV Advanced Monitoring Systems Center (U.S.), eds. Abraxis Ecologenia® 17[beta symbol]-estradiol (E2) microplate Enzyme-Linked Immunosorbent Assay (ELISA) test kits. U.S. Environmental Protection Agency, Office of Research and Development, 2009.

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Darcel, Colin. A collection of essays on comparative medicine: And a bibliography of references to the ELISA test. Palliser Animal Health Laboratories, Ltd., 1996.

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O'Connor, Glenda. Use of ELISA for monitoring bacterial kidney disease in naturally spawning chinook salmon. Oregon Dept. of Fish and Wildlife, 2006.

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Berlin, Technische Universität, ed. Beitrag zur Analytik der Vitamine B12, Biotin und Folsäure: Bestimmung der Vitamine in vitaminierten Lebensmitteln mittels Enzyme-Linked Immunosorbent Assay (ELISA) und competitivem Bindungsprotein Assay (CBPA). [s.n.], 1991.

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Capítulos de livros sobre o assunto "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Crowther, John. "Enzyme Linked Immunosorbent Assay (ELISA)." In Springer Protocols Handbooks. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_37.

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Crowther, John R. "Enzyme- Linked Immunosorbent Assay (ELISA)." In Springer Protocols Handbooks. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_46.

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Gooch, Jan W. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13676.

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Tabatabaei, Mahdis Sadat, and Marya Ahmed. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2376-3_10.

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Mandal, Santi M., and Debarati Paul. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Automation and Basic Techniques in Medical Microbiology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2372-5_7.

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Konstantinou, George N. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6925-8_7.

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Walker, John M. "The Enzyme Linked Immunosorbent Assay (ELISA)." In Techniques in Molecular Biology. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9799-5_4.

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Garvey, Justine S., Dafydd G. Thomas, and Harry J. Linton. "Enzyme-Linked Immunosorbent Assay (ELISA) for Metallothionein." In Experientia Supplementum. Birkhäuser Basel, 1987. http://dx.doi.org/10.1007/978-3-0348-6784-9_31.

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Sam-Yellowe, Tobili Y. "Exercise 15: Enzyme-Linked Immunosorbent Assay (ELISA)." In Immunology: Overview and Laboratory Manual. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-64686-8_39.

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Butler, J. E., J. H. Peterman, and T. E. Koertge. "The Amplified Enzyme-Linked Immunosorbent Assay (a-ELISA)." In Enzyme-Mediated Immunoassay. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5012-5_14.

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Trabalhos de conferências sobre o assunto "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Sugihara, T., J. Takamatsu, T. Kamiya, H. Saito, K. Kimata, and K. Kato. "A SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR SERUM LAMININ." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643554.

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Laminin, a large glycoprotein, is a major and specific component of basement membrane. There were little or no circulating laminin in normal persons, although some recent reports have showed increased values in various diseases(ex. diabetes mellitus and liver disease) by a radioimmunoassay(RIA) using laminin fragment. The minimum detectable sensitivity of RIA was reported to be 20 ng/ml of serum sample. We describe here a more sensitive immunoassay system, and also the concentrations of laminin in sera from healthy subjects and patients. A sandwich ELISA method for measurement of laminin was e

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Lee, Wen-Wai, Kuan-Ju Tseng, and Ju-Nan Kuo. "PDMS microlens fabricated for compact disk enzyme-linked immunosorbent assay (CD-ELISA) applications." In 2010 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2010). IEEE, 2010. http://dx.doi.org/10.1109/nems.2010.5592184.

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Uchino, Takashi. "Graphene-based surface-enhanced Raman spectroscopy (SERS) for enzyme-linked immunosorbent assay (ELISA)." In Plasmonics in Biology and Medicine XXI, edited by Tuan Vo-Dinh, Ho-Pui A. Ho, and Krishanu Ray. SPIE, 2024. http://dx.doi.org/10.1117/12.2691655.

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MacGregor, I. R., N. A. Booth, N. R. Hunter, and B. Bennet. "QUANTIFICATION OF ENDOTHELIAL PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) ANTIGEN BY AN ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644450.

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An ELISA to measure PAI-1 antigen has been developed using PAI-1 purified from human endothelial cell conditioned medium and a monospecific antiserum raised against it in the rabbit. Test and standard samples diluted in assay buffer containing non-immune rabbit serum were incubated in microplate wells coated with anti-PAI-1 IgG. Then biotinylated anti-PAI-1 IgG was added to the wells followed by a streptavidin-biotinylated horseradish peroxidase complex. Tetramethylbenzidine was used as substrate and the optimised ELISA had a detection limit of 0.2 ng PAI-1 ml-1 sample, using purified PAI-1 of

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Grøndahl-HANSEN, J., N. Agerlin, L. S. Nielsen, and K. Danø. "SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644425.

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An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactiv

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Metwali, Nervana, and Peter S. Thorne. "Development Of An Enzyme-Linked Immunosorbent Assay (ELISA) For Bacterial Peptidoglycan In Air And Dust Samples." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a4643.

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Czerchaujski, L., V. Hornsey, C. Prowse, and H. Bessos. "CROSS-REACTIV7E Fl/III :C IN THE RABBIT: A POTENTIAL ANIMAL MODEL FOR FUIII:C STUDIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644036.

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A one step Enzyme-linked immunosorbent assay (ELISA) incorporating two anti-human FVIII :Ag monoclonal antibodies (MAbs) was used to determine FUIIIrAg in rabbits. Initial examinations showed the presence of cross-reactive FUIII:C in rabbit serum, plasma, and homogenates of normal rabbit liver, lung and spleen. Detailed investigation of normal rabbit plasma, and plasma depleted with Sephacryl S1000-anti FVIII :C MAbs and irrelevant MAbs (as control) using the ELISA, a chromogenic Fl/III:C activity assay, an immunoradiometric assay (IRMA) using human antibody, and fast protein liquid chromatogr

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Yamamura, Yuki, Tomoaki Iwai, and Yutaka Shokaku. "ELISA Analysis of Latex Allergens on Wear Particles of Natural Rubber During Rolling-Sliding Contact." In ASME/STLE 2009 International Joint Tribology Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/ijtc2009-15237.

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The wear particles generated from a tire model made of vulcanized natural rubber were analyzed using ELISA (Enzyme-Linked Immunosorbent Assay). Antibodies to 4 different allergens were used to analyze the extracted proteins from the wear particles. Latex allergens were detected when the slip ratio was large. However, no clear relationship between the latex allergen content extracted from the wear debris and either the average size or the number of wear particles was observed.

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Dutta, Debashis, and Naoki Yanagisawa. "Microfluidic Devices for Enhancing the Sensitivity of ELISA Methods." In ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2011. http://dx.doi.org/10.1115/icnmm2011-58284.

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Enzyme-linked immunosorbent assays (ELISA) are critically important tools in biological research, allowing the presence and concentrations of a wide variety of key biochemical intermediates to be determined. While the signal amplification that is the core advantage of ELISA methods is impressive, it is nevertheless the case that it is insufficient for some particularly demanding challenges in terms of sensitivity, assay time, or sample size. In this paper, we discuss three different approaches developed in our laboratory that can improve the sensitivity of ELISA methods by 2–3 orders of magnit

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Takehara, H., K. Miyazawa, T. Noda, et al. "A CMOS Image Sensor Having Stacked Photodiodes for Lensless Observation System of Digital Enzyme-linked Immunosorbent Assay (ELISA)." In 2013 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2013. http://dx.doi.org/10.7567/ssdm.2013.g-4-4.

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Relatórios de organizações sobre o assunto "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

จันทร์ศิริพรชัย, นิวัตร. การพัฒนาวิธี ELISA และการยับยั้งการตกตะกอนกับเม็ดเลือดแดงสำหรับตรวจสอบแอนติบอดีต่อเชื้อไวรัสหลอดลมอักเสบติดต่อ. จุฬาลงกรณ์มหาวิทยาลัย, 2008. https://doi.org/10.58837/chula.res.2008.75.

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โรคหลอดลมอักเสบติดต่อ เป็นโรคติดเชื้อไวรัสในไก่ ก่อให้เกิดความเสียหายทางเศรษฐกิจต่ออุตสาหกรรมการเลี้ยงไก่เป็นอย่างมาก การตรวจหาระดับภูมิคุ้มกันต่อไวรัสชนิดนี้จากซีรัมไก่มีความสำคัญ และถือเป็นการปฏิบัติงานปรกติของนายสัตวแพทย์ผู้ควบคุมฟาร์มสัตว์ปีก เพื่อจุดประสงค์ในการตรวจติดตามระดับภูมิคุ้มกันต่อโรคหลอดลมอักเสบติดต่อที่ถ่ายทอดมาจากแม่ ว่าอยู่ในระดับที่สามารถป้องกันโรคในลูกไก่ได้หรือไม่ และยังเป็นการบอกช่วงเวลาที่เหมาะสมในการจัดทำโปรแกรมวัคซีน ปัจจุบัน ห้องปฏิบัติการส่วนใหญ่ นิยมใช้วิธี Enzyme-linked immunosorbent assay (ELISA) โดยนำเข้าชุดทดสอบสำเร็จรูปเชิงพาณิชย์จากต่างประเทศ ดังนั้น งานวิจัยจ

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Khongchareonporn, Nanthika, Songchan Puthong, Anumart Buakeaw, Kittinan Komolpis, and Umaporn Pimpitak. Production of monoclonal antibody against oxytetracycline for developing enzyme-linked immunosorbent assay (ELISA) test kit, 2nd year. Chulalongkorn University, 2015. https://doi.org/10.58837/chula.res.2015.100.

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In Thailand, oxytetracyline (OTC) has been used in the protection and treatment of infected shrimps. The misuse of OTC could lead to OTC residues in shrimps destined for consumption. To prevent consumers from exposure to drug residues and increase of drug resistance pathogens, several food safety authorities in many countries have set the maximum residue limits (MRLs) for OTC and enforced the surveillance detection programs. Therefore, an effective screening method is essential to detect OTC residue in food. Enzyme-linked immunosorbent assay (ELISA) is the suitable methods for screening a larg

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ภู่ทอง, ทรงจันทร์, อุมาพร พิมพิทักษ์, กิตตินันท์ โกมลภิส, อณุมาศ บัวเขียว та พลกฤษ์ แสงวณิช. การพัฒนาชุดตรวจวิเคราะห์โพรเจสเทอโรนในน้ำนมโคด้วยวิธีเอนไซม์ลิงค์อิมมิวโนซอร์เบนด์แอสเสย์ : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2011. https://doi.org/10.58837/chula.res.2011.120.

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โพรเจสเทอโรนเป็นสเตอรอยด์ฮอร์โมน (C21 steroid, pregn-4-ene-3, 20 dione) ซึ่งถูกผลิตและหลั่งมาอยู่ในน้าเลือดและน้านมโดยคอร์ปัสลูเทียมหลังจากการตกไข่ระหว่างวงรอบการเป็นสัด เป็นที่ทราบกันว่าระดับความเข้มข้นของโพรเจสเทอโรนสามารถใช้ระบุการเข้าสู่วงรอบการเป็นสัดและการตั้งครรภ์ของโคได้โดยมีความถูกต้องอยู่ที่ 75 – 96% ดังนั้นวิธีการตรวจหาทางระบบภูมิคุ้มกันจึงได้รับการพัฒนาและนาไปใช้ในการตรวจหาระดับฮอร์โมนโพรเจสเทอโรน โดยการใช้เทคนิค enzyme-linked immunosorbent assay (ELISA) ซึ่งถือว่าเป็น หนึ่งในวิธีการที่เหมาะสมที่สุด เนื่องจากให้ ความไวสูง สามารถทาได้ง่าย และมีความจาเพาะสูง ในการศึกษานี้ได้พัฒนาชุดต

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โกมลภิส, กิตตินันท์, นันทิกา คงเจริญพร, ทรงจันทร์ ภู่ทอง, อณุมาศ บัวเขียว та ธนาภัทร ปาลกะ. การผลิตโมโนโคลนอลแอนติบอดีสำหรับตรวจวัดอะฟลาทอกซินเอ็ม1 ด้วยวิธีเอนไซม์ลิงก์อิมมิวโนซอร์เบนท์แอสเสย์. จุฬาลงกรณ์มหาวิทยาลัย, 2015. https://doi.org/10.58837/chula.res.2015.97.

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อะฟลาทอกซิน (aflatoxin, AF) เป็นกลุ่มสารพิษที่เกิดจากราซึ่งอาจปนเปื้อนอยู่ในพืชที่ใช้เป็นอาหารสัตว์ อะฟลาทอกซินหลักที่พบได้แก่ อะฟลาทอกซินบี1 อะฟลาทอกซินบี2 อะฟลาทอกซินจี1 และอะฟลาทอกซินจี2 เมื่อสัตว์ได้รับอะฟลาทอกซินชนิด บี1 (AFB1) เข้าสู่ร่างกายจะเกิดการเปลี่ยนรูปเป็นอะฟลาทอกซินชนิด เอ็ม1 (AFM1) ที่บริเวณตับและหลั่งออกมายังต่อมน้ำนม ซึ่งสารนี้เป็นสารก่อมะเร็งชนิดหนึ่ง ดังนั้นการตรวจหาปริมาณของ AFM1 ที่มีอยู่ในผลิตภัณฑ์นมจึงมีความจำเป็น ในการตรวจวัดปริมาณ AFM1 นั้นทำได้หลายวิธี โดยวิธีที่นิยมใช้ตรวจคัดกรองตัวอย่างจำนวนมากได้แก่วิธีเอนไซม์ลิงก์อิมมิวโนซอร์เบนท์แอสเสย์ (enzyme-linked immunosorb

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โกมลภิส, กิตตินันท์, ธนาภัทร ปาลกะ, นันทิกา คงเจริญพร, ทรงจันทร์ ภู่ทอง та อณุมาศ บัวเขียว. การผลิตโมโนโคลนอลแอนติบอดีสำหรับตรวจวัดอะฟลาทอกซินเอ็ม1 ด้วยวิธีเอนไซม์ลิงก์อิมมิวโนซอร์เบนท์แอสเสย์. จุฬาลงกรณ์มหาวิทยาลัย, 2014. https://doi.org/10.58837/chula.res.2014.91.

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อะฟลาทอกซิน (aflatoxin, AF) เป็นกลุ่มสารพิษที่เกิดจากราซึ่งอาจปนเปื้อนอยู่ในพืชที่ใช้เป็นอาหารสัตว์ อะฟลาทอกซินหลักที่พบได้แก่ อะฟลาทอกซินบี1 อะฟลาทอกซินบี2 อะฟลาทอกซินจี1 และอะฟลาทอกซินจี2 เมื่อสัตว์ได้รับอะฟลาทอกซินชนิด บี1 (AFB₁เข้าสู่ร่างกายจะเกิดการเปลี่ยนรูปเป็นอะฟลาทอกซินชนิด เอ็ม1 (AFM₁) ที่บริเวณตับและหลั่งออกมายังต่อมน้ำนม ซึ่งสารนี้เป็นสารก่อมะเร็งชนิดหนึ่ง ดังนั้นการตรวจหาปริมาณของ AFM₁ ที่มีอยู่ในผลิตภัณฑ์นมจึงมีความจำเป็น ในการตรวจวัดปริมาณ AFM₁ นั้นทำได้หลายวิธี โดยวิธีที่นิยมใช้ตรวจคัดกรองตัวอย่างจำนวนมากได้แก่วิธีเอนไซม์ลิงก์อิมมิวโนซอร์เบนท์แอสเสย์ (enzyme-linked immunosorben

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ไชยวงศ์คต, อาคม, ภาวพันธ์ ภัทรโกศล та สมชัย นิรุตติศาสน์. การตรวจหาภาวะการเปลี่ยนแปลงการควบคุมเหนือพันธุกรรมในตัวอย่างเซลล์ปากมดลูกที่มีความผิดปกติระดับต่างๆ : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2016. https://doi.org/10.58837/chula.res.2016.27.

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โรคมะเร็งปากมดลูกเป็นโรคมะเร็งที่พบมากเป็นอันดับสองในผู้หญิงทั่วโลก โดยเฉพาะในประเทศกำลังพัฒนา ซึ่งรวมประเทศไทยด้วย มีการศึกษาพบภาวะ global DNA hypomethylation ในเซลล์มะเร็งชนิดต่างๆ รวมถึงมะเร็งปากมดลูกด้วย การศึกษานี้จึงสนใจพัฒนาวิธี Enzyme linked immunosorbent assay (ELISA) ในการตรวจ global DNA methylation เพื่อตรวจกรองมะเร็งปากมดลูกซึ่งวิธี ELISA เหมาะสมกับประเทศกำลังพัฒนา วิธีดำเนินการวิจัย การศึกษานี้พัฒนาวิธี ELISA ในการตรวจ global DNA methylation พร้อมเปรียบเทียบผลกับวิธี bisulfite LINE1 pyrosequencing โดยใช้ตัวอย่างดีเอ็นเอที่สกัดจากเซลล์ปากมดลูกที่มีความผิดปกติระดับต่างๆ ผลการวิจัย โ

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สิโรตมรัตน์, สัตถาพร, เครือวัลย์ พลจันทร та อัมพร ตันประเสริฐ. การตรวจหาดีเอ็นเอไวรัสตับอักเสบบีในซีรัมหญิงตั้งครรภ์ด้วยวิธี Polymerase chain reaction. จุฬาลงกรณ์มหาวิทยาลัย, 2001. https://doi.org/10.58837/chula.res.2001.29.

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นำตัวอย่างซีรัมหญิงตั้งครรภ์ทั้งหมด 512 ตัวอย่าง มาตรวจหาดีเอ็นเอส่วน S gene ของไวรัสตับอักเสบบี (HBV-DNA) ซึ่งเป็นแถบดีเอ็นเอขนาด 431 คู่เบส ด้วยวิธี Polymerase Chain Reaction (PCR) ศึกษาควบคู่กับการตรวจหาดัชนีในซีรัม (seromarkers) อื่นๆ ด้วยวิธีทางอิมมูโนแอสเสย์ ได้แก่ แอนติเจนชนิดผิวของไวรัสตับอักเสบบี (HBsAg) ภูมิต้านทานต่อแอนติเจนชนิดผิวของไวรัส (Anti-HBs) และ ภูมิต้านทานต่อส่วยแกน (core) ของอนุภาคไวรัส (Anti-HBC) การตรวจหา HBsAgใช้ 2 วิธี คือ Chemiluminescent immunoassay : Abbott prism HBsAg ประเทศเยอรมัน และ Enzyme-Linked Immunosorbent Assay (ELISA) ซึ่งพัฒนาโดยกรมวิทยาศาสตร์ กระทรวงสาธ

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López-Valverde, Nansi, Antonio López-Valverde, Ana Suarez, Bruno Macedo de Sousa, and Juan Manuel Aragoneses. Association of gastric infection and periodontal disease through Helicobacter pylori as a common denominator: A systematic review and meta-analysi. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2021. http://dx.doi.org/10.37766/inplasy2021.10.0097.

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Review question / Objective: Is gastric helicobacter pylori infection related to periodontal diseases? Condition being studied: Therefore, the aim of this systematic review and meta-analysis was to identify and analyze clinical studies to determine the direct correlation between Helicobacter Pylori gastric infection andPeriodontal Disease. Study designs to be included: Clinical studies that provided data on Helicobacter Pylori infection in both the stomach and oral cavity, confirmed by polymerase chain reaction (PCR), rapid urease test (RUT) or enzyme-linked immunosorbent assay (ELISA). Clinic

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กระหม่อมทอง, อินทิรา, วารี นิยมธรรม та เกรียงศักดิ์ พูนสุข. การใช้อีไลซ่าเทคนิคในการตรวจแอนติบอดีต่อเชื้อซาลโมเนลลา เอนเตอริทิดิส ในไก่ : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 1999. https://doi.org/10.58837/chula.res.1999.68.

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ได้ทำการพัฒนาวิธีทดสอบแอนติบดีต่อเชื้อ ซาลโมเนลลา เอนเตอริทิดิส (Salmonella enteritidis) ในฝูงไก่ โดยวิธีอินไดเร็ค อีไลซ่า (Indirect Enzyme-linked immunosorbent Assay-ELISA) เปรียบเทียบกับวิธี อินไดเร็คฮีแมกกลูติเนชั่น (Indorect Hemagglutlinaton - IHA) โดยใช้สารสกัดไลโปโพลีแซคคาไรด์ (Lipopolysaccharide - LPS) จากผนังเซลล์ของเชื้อเป็นแอนติเจนในการทดสอบทั้งวิธี ELISA และ IHA ค่าตัดสินบวกและลบของวิธี ELISA คือ 0.34 ได้จากค่า O.D (optical density) เฉลี่ยของซีรั่มไก่ SPF (specific pathogen free) บวกกับ 3 เท่าของส่วนเบี่ยงเบนมาตรฐาน ส่วนวิธี IHA ซีรั่มไตเตอร์ 1:16 ขึ้นไปตัดสินเป็นบวก ทำการทดลองในไก่

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Torrungruang, Kitti, and Suchada Chutimawarapun. Antimicrobial activity against periodontopathic bacteria, cell toxicity against gingival fibroblasts and antinflammatory effect of crude extract from mangosteen. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.15.

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Extract from mangosteen pericarp has demonstrated various pharmacological activities including anti-bacterial, anti-inflammatory and anti-fungal. It may have potential for the treatment of periodontal disease, which is a chronic inflammatory disease caused by anaerobic bacteria. The aim of this study was 1) to investigate the toxicity of mangosteen extract to human gingival fibroblast, 2) to examine the anti-bacterial activity of the extract against periodontopathic bacteria including P. gingivalis and A. actinomycetemcomitans, and 3) to examine the inhibitory effect of the extract on PGE[subs

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