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Artigos de revistas sobre o tema "Psychosocial Enzyme-linked immunosorbent assay (ELISA)"

Autor: Grafiati

Publicado: 15 de setembro de 2021

Última modificação: 30 de julho de 2025

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1

Guo, Huirong, Yuming Ren, Bailing Huang, Junru Wang, Xuhuang Yang, and Yali Wang. "Psychological Status, Compliance, Serum Brain-Derived Neurotrophic Factor, and Nerve Growth Factor Levels of Patients with Depression after Augmented Mindfulness-Based Cognitive Therapy." Genetics Research 2022 (January 4, 2022): 1–5. http://dx.doi.org/10.1155/2022/1097982.

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Objective. Mindfulness-based cognitive therapy (MBCT) is a cost-effective psychosocial program that prevents relapse/recurrence in major depression. The present study aimed to analyze the effects of augmented MBCT along with standard treatment dominated by pharmacotherapy on psychological state, compliance, brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) expression levels in patients with depression. Methods. A total of 160 eligible patients with depression in The First Affiliated Hospital of Zhengzhou University were included in this study. The study randomly assigned

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Monteleone, P., P. Scognamiglio, B. Canestrelli, I. Serino, A. M. Monteleone та M. Maj. "Asymmetry of salivary cortisol and α-amylase responses to psychosocial stress in anorexia nervosa but not in bulimia nervosa". Psychological Medicine 41, № 9 (2011): 1963–69. http://dx.doi.org/10.1017/s0033291711000092.

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BackgroundThe stress response involves the activation of the hypothalamic–pituitary–adrenal (HPA) axis and the sympathetic nervous system (SNS). As a role for stress in determining of the onset and the natural course of eating disorders (EDs) has been proposed, the study of the psychobiology of the stress response in patients with anorexia nervosa (AN) and bulimia nervosa (BN) should be helpful in understanding the pathophysiology of these disorders. The two neurobiological components of the stress response can be easily explored in humans by the measurement of salivary cortisol and α-amylase

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LI, Bei, Xiao-hong DUAN, Jin-feng WU, et al. "Impact of psychosocial stress on airway inflammation and its mechanism in a murine model of allergic asthma." Chinese Medical Journal 126, no. 2 (2013): 325–34. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20120685.

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Background It has already been recognized that psychosocial stress evokes asthma exacerbation; however, the mechanism of how stress gets inside the body is not clear. This study aimed to observe the impact of psychosocial stress on airway inflammation and its mechanism in the ovalbumin-induced asthmatic mice combined with social disruption stress. Methods Thirty-six male BALB/c mice were randomly divided into: control group, asthma group (ovalbumin-induced), asthma plus social disruption stress group (SDR), and SDR group. The open field video tracking system was used to assess animal behaviors

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Ningrum, Aji Mustika, Prananda Surya Airlangga, Wiwiek Indriyani Maskoep, and Herdiani Sulistyo Putri. "Relationship of quinolinic acid and serotonin with depression and pain degree in cancer pain patients: a cross-sectional study." Bali Medical Journal 12, no. 3 (2023): 2681–84. http://dx.doi.org/10.15562/bmj.v12i3.4697.

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Link of Video Abstract: https://youtu.be/zg5vubHMcDY Introduction: Chronic pain requires a thorough assessment if it persists in patients with cancer. Depressive disorder is one of the psychosocial problems that can occur in cancer patients. Depressive disorders affect up to 34.4% of patients with cancer in Indonesia, causing increased morbidity and complicating the management of patients. Quinolinic acid (QUIN) and serotonin (5-HT) are chemicals that can affect pain perception and depressive disorders. This study aims to analyze the relationship between quinolinic acid and serotonin levels wi

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Dita, Maya Chandra. "Enzyme Linked Immunosorbent Assay (ELISA)." Natural Sciences Engineering and Technology Journal 1, no. 2 (2021): 29–38. http://dx.doi.org/10.37275/nasetjournal.v1i2.6.

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Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in many industries. ELISA has been the system of choice when testing soluble antigens and antibodies. EIA / ELISA uses the basic immunological concept of antigen binding to specific antibodies, which allows the detection of small amounts of antigens such as proteins, peptides, hormones or antibodies in fluid samples. In all protocols, solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled with the enzyme.

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Engvall, Eva. "The ELISA, Enzyme-Linked Immunosorbent Assay." Clinical Chemistry 56, no. 2 (2010): 319–20. http://dx.doi.org/10.1373/clinchem.2009.127803.

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Lequin, Rudolf M. "Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)." Clinical Chemistry 51, no. 12 (2005): 2415–18. http://dx.doi.org/10.1373/clinchem.2005.051532.

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Abstract This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient sampl

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Hidayat, Rachmat, and Patricia Wulandari. "Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 2 (2021): 352–58. http://dx.doi.org/10.32539/bsm.v5i2.228.

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A B S T R A C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle of antigen-antibody binding. In the ELISA technique, antigenimmobilization will be carried out on a solid surface, then bound withantibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the formof a color change will be formed due to the reaction between the enzyme andthe substrate.

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Hidayat, Rachmat, and Patricia Wulandari. "Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 5 (2021): 447–53. http://dx.doi.org/10.32539/bsm.v5i5.228.

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ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

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Kohl, Thomas O., and Carl A. Ascoli. "Direct Competitive Enzyme-Linked Immunosorbent Assay (ELISA)." Cold Spring Harbor Protocols 2017, no. 7 (2017): pdb.prot093740. http://dx.doi.org/10.1101/pdb.prot093740.

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Kohl, Thomas O., and Carl A. Ascoli. "Indirect Competitive Enzyme-Linked Immunosorbent Assay (ELISA)." Cold Spring Harbor Protocols 2017, no. 7 (2017): pdb.prot093757. http://dx.doi.org/10.1101/pdb.prot093757.

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Shah, Karishma, and Panagiotis Maghsoudlou. "Enzyme-linked immunosorbent assay (ELISA): the basics." British Journal of Hospital Medicine 77, no. 7 (2016): C98—C101. http://dx.doi.org/10.12968/hmed.2016.77.7.c98.

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Cleary, J. D., S. W. Chapman, J. Deng, and C. J. Lobb. "Amphotericin B enzyme-linked immunosorbent assay." Antimicrobial Agents and Chemotherapy 40, no. 3 (1996): 637–41. http://dx.doi.org/10.1128/aac.40.3.637.

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Our purpose was to develop and characterize an enzyme-linked immunosorbent assay (ELISA) which could measure the concentration of amphotericin B in serum. Amphotericin B was assayed by competition ELISA. Multiwell ELISA plates coated with amphotericin B (1.0 micrograms/ml) conjugated to bovine serum albumin were used to test replicates of serum samples spiked with amphotericin B. Purified rabbit polyclonal antibody against amphotericin B (1.4 micrograms/ml) was added subsequent to the instillation of samples spiked with unknown amounts of amphotericin B. Experiments were performed to test the

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Tarassishin, Leonid. "The Evolution of the Enzyme Immunoassay/Enzyme-Linked Immunosorbent Assay." Journal of Proteomics and Genomics Research 2, no. 3 (2021): 13–17. http://dx.doi.org/10.14302/issn.2326-0793.jpgr-21-3917.

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50 years ago the Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay, mostly known as ELISA was developed. This is a powerful but simple method that is very widely used in the diagnostic practice, as well as in biomedical research. During this time a number of ELISA modification were developed that significantly increased its properties, especially the senstivity, such as avidin-biotin assay, immuno-PCR, nano-ELISA and finally, the digital ELISA. This short review describes the principles of ELISA and the evolution from a conventional assay to the modern ultra-sensitive method. Most of the im

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Dillen, L., J. De Block, L. Van Lear, and W. De Potter. "Enzyme-linked immunosorbent assay for chromogranin A." Clinical Chemistry 35, no. 9 (1989): 1934–38. http://dx.doi.org/10.1093/clinchem/35.9.1934.

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Abstract This is an enzyme-linked immunosorbent assay (ELISA) for determining chromogranin A (CGA) with use of a monoclonal antibody. CGA was isolated from bovine chromaffin granules. The analytical ELISA procedure for bovine CGA was developed and optimized. Typical standard curves ranged from 500 pg to 500 ng of CGA. We then studied human plasma CGA-immunoreactivity as measured by this assay. The curve for dilutions of human plasma paralleled the standard curve for bovine CGA. The intra-assay coefficient of variation for determination of human plasma CGA was 4.56%, indicating that reliable de

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Figuerêdo-Silva, José, Yoshimasa Kaneda, Hiroshi Tachibana, et al. "Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods." Revista do Instituto de Medicina Tropical de São Paulo 33, no. 3 (1991): 193–98. http://dx.doi.org/10.1590/s0036-46651991000300005.

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A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagn

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She, Zhen. "Application of enzyme-linked immunosorbent assay in cancer detection." Transactions on Materials, Biotechnology and Life Sciences 3 (March 24, 2024): 125–30. http://dx.doi.org/10.62051/ybp2dp18.

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The enzyme-linked immunosorbent assay (ELISA) has emerged as an indispensable method in the realm of cancer detection., serving as a significant factor in the timely identification, prediction, and surveillance of many forms of cancer. ELISA as a highly sensitive immunological assay, utilizes the binding affinity between antibodies and antigens to detect and quantify cancer-associated biomolecules, such as tumor markers, oncogenic proteins, and specific antibodies. The recently adopted methodology facilitates the identification of cancer-associated biomarkers in various types of samples, such

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Spinner, Sabine, Georg Stöffler, and Ernst Fink. "Quantitative enzyme-linked immunosorbent assay (ELISA) for hirudin." Journal of Immunological Methods 87, no. 1 (1986): 79–83. http://dx.doi.org/10.1016/0022-1759(86)90346-7.

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Ghurafa, Rinaldi, Denny Widaya Lukman, and Hadri Latif. "Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW)." Jurnal Veteriner 20, no. 1 (2019): 30. http://dx.doi.org/10.19087/jveteriner.2019.20.1.30.

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Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results

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XU, Yi-Chun, Guang S. Zhang, and Fun S. Chu. "Enzyme-Linked Immunosorbent Assay for Deoxynivalenol in Corn and Wheat." Journal of AOAC INTERNATIONAL 71, no. 5 (1988): 945–49. http://dx.doi.org/10.1093/jaoac/71.5.945.

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Abstract The availability of antibody against deoxynivalenol (DON) triacetate (Tri-Ac-DON) has enabled development of a direct enzyme-linked immunosorbent assay (ELISA) and an indirect ELISA for DON in corn and wheat. In both assays, DON is extracted from the sample with acetonitrile- water, reacted with acetic anhydride to form Tri- Ac-DON, and diluted in phosphate buffer for analysis. Direct ELISA was found to be the more sensitive procedure. Fewer interferences are evidenced, and the assay is less time consuming than is indirect ELISA. For direct ELISA, recovery of 10-1000 ppb DON added to

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Danilov, Dmitry V., Olga A. Shmeleva, Alexander S. Lunin, Liubov I. Kozlovskaya, Anastasia N. Piniaeva, and Anna A. Shishova. "Development of a biosafe ELISA-based platform for assessing immunogenicity in the production of an inactivated whole-virion coronavirus vaccine." Medical academic journal 2, no. 2 (2022): 163–69. http://dx.doi.org/10.17816/maj108717.

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BACKGROUND: SARS-CoV-2 vaccine immunogenicity is evaluated in neutralization test with live virus. It is performed in a biosafety level 3 zone because requires live virus stage. Therefore, control laboratories should be certified for this class of work. The development of technology based on enzyme-linked immunosorbent assay as an analogue of the neutralization reaction makes it possible to create an immunobiological product in a shorter time and in conditions without special requirements for control laboratories. AIM: Development of an enzyme-linked immunosorbent assay for assessing SAR

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Khan, Muddasir, Syed Hussain Shah, Muhammad Salman, Mr Abdullah, Fawad Hayat, and Sajeela Akbar. "Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview." Global Journal of Medical, Pharmaceutical, and Biomedical Update 18 (February 8, 2023): 1. http://dx.doi.org/10.25259/gjmpbu_77_2022.

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Enzyme-linked immunosorbent assay (ELISA) technique measures antigens, antibodies, and protein reactions in biological samples by enzymatic reactions. The chemiluminescence immunoassay (CLIA) technique determines sample concentrations based on the intensity of the light emitted by a chemical and biological reaction. This review provides an overview to understand the ELSIA and CLIA methods with their types and comparison. ELISA and CLIA methods were compared based on previous literature studies. In conclusion, CLIA is found highly sensitive, specific, and rapid, as compared to ELISA, but CLIA i

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Sathe, Manisha, Shruti Srivastava, Sumit Agrawal, and Ramrao Ghorpade. "Effect of Spacer and the Enzyme-Linked Immunosorbent Assay." Defence Science Journal 66, no. 5 (2016): 471. http://dx.doi.org/10.14429/dsj.66.10700.

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The effect of spacers and the enzyme-linked immunosorbent assay (ELISA) formats on the functional parameters of assays such as lower detection limit, inhibitory concentration at 50 per cent (IC50), and specificity were studied. Enzyme conjugates having hydrophobic and hydrophilic spacers were prepared using O-isopropyl methylphosphonic acid (IMPA) and horseradish peroxidase (HRP) as an enzyme label. Comparison was made with reference to enzyme conjugate without any spacer. The present investigation revealed that the presence of a hydrophilic spacer in the enzyme conjugate significantly improve

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Chu, Fun S., and Titan S. L. Fan. "Indirect Enzyme-Linked Immunosorbent Assay for Saxitoxin in Shellfish." Journal of AOAC INTERNATIONAL 68, no. 1 (1985): 13–16. http://dx.doi.org/10.1093/jaoac/68.1.13.

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Abstract An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of saxitoxin (STX). Antibodies against STX were demonstrated in rabbits 5 weeks after immunizing with STX-bovine serum albumin (STX-HCHO-BSA). In the ELISA, STX-HCHO-BSA or polylysine-STX was coated onto the microtiter plate, followed by incubation with standard toxin and anti-STX antibody. The amount of antibody bound to the solid phase was determined by incubation with goat anti-rabbit IgG peroxidase conjugate and a reaction with chromogenic substrate. Competitive indirect ELISA revealed that the a

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Ginige, Shamila, Robert Flower, James Daly, and Tanya Powley. "Enzyme-linked immunosorbent assay (ELISA) for anti-D quantification." Pathology 53 (July 2021): S44—S45. http://dx.doi.org/10.1016/j.pathol.2021.06.091.

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Karpinski, K. F. "Optimality Assessment in the Enzyme-Linked Immunosorbent Assay (ELISA)." Biometrics 46, no. 2 (1990): 381. http://dx.doi.org/10.2307/2531443.

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PRICE, LAURA J., and ROGER HARRISON. "Sensitive enzyme linked immunosorbent assay (ELISA) for xanthine oxidase." Biochemical Society Transactions 21, no. 2 (1993): 102S. http://dx.doi.org/10.1042/bst021102s.

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Mendoza, L. G., P. McQuary, A. Mongan, R. Gangadharan, S. Brignac, and M. Eggers. "High-Throughput Microarray-Based Enzyme-Linked Immunosorbent Assay (ELISA)." BioTechniques 27, no. 4 (1999): 778–88. http://dx.doi.org/10.2144/99274rr01.

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Snyder, D. B. "Latest Developments in the Enzyme-Linked Immunosorbent Assay (ELISA)." Avian Diseases 30, no. 1 (1986): 19. http://dx.doi.org/10.2307/1590607.

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NAMBIAR, P. T. C., and V. ANJAIAH. "Enumeration of rhizobia by enzyme-linked immunosorbent assay (ELISA)." Journal of Applied Bacteriology 58, no. 2 (1985): 187–93. http://dx.doi.org/10.1111/j.1365-2672.1985.tb01446.x.

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Elder, P. A., and J. G. Lewis. "An enzyme-linked immunosorbent assay (ELISA) for plasma testosterone." Journal of Steroid Biochemistry 22, no. 5 (1985): 635–38. http://dx.doi.org/10.1016/0022-4731(85)90217-1.

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Lewis, J. G., and P. A. Elder. "An enzyme-linked immunosorbent assay (ELISA) for plasma cortisol." Journal of Steroid Biochemistry 22, no. 5 (1985): 673–76. http://dx.doi.org/10.1016/0022-4731(85)90222-5.

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Madeali, Mun Imah, and Nurhidayah Nurhidayah. "KIT ENZYME-LINKED IMMUNOSORBENT ASSAY UNTUK DETEKSI WSSV PADA UDANG." Jurnal Riset Akuakultur 6, no. 1 (2011): 131. http://dx.doi.org/10.15578/jra.6.1.2011.131-137.

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Komponen dasar yang penting dan menentukan keberhasilan pengendalian suatu penyakit dalam bidang perikanan adalah informasi tentang patogen secara dini, cepat, dan akurat, serta epidemi penyakit di lapangan. Teknik serologi, khususnya ELISA, merupakan salah satu teknik yang menjanjikan untuk keperluan tersebut, karena relatif mudah dan murah, serta berpeluang untuk digunakan secara langsung di lapangan. Telah dilakukan uji lapang terhadap perangkat Kit ELISA yang telah diproduksi oleh Balai Riset Perikanan Budidaya Air Payau. Hasil uji lapang menunjukkan bahwa Kit ELISA dapat digunakan untuk m

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Fiscus, Susan A., Michael M. Mildbrand, John C. Gordon, Yoshio A. Teramoto, and Scott Winston. "Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus." American Journal of Veterinary Research 46, no. 4 (1985): 859–63. https://doi.org/10.2460/ajvr.1985.46.04.859.

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SUMMARY A rapid screening assay for determining antibodies to canine parvovirus in dog serum using monoclonal antibodies and enzyme-linked immunosorbent assay (elisa) technology was developed. The elisa could be read visually, and the results correlated well with serum neutralization (sn) and hemagglutination inhibition (hi) titers. Sera with sn ≤ 1:4 or hi ≤ 1:10 had an 87.9% correlation with elisa and sera with sn ≥ 1:64 or hi ≥ 1:80 had a 94.4% correlation. The assay took only 10 to 15 minutes to perform and did not require specialized equipment. The elisa should be useful in monitoring dog

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Dildar, Shabnam, Asma Danish, Mehjabeen Imam, Arshi Naz, and Tahir Sultan Shamsi. "Diagnostic Performance of Three Serological Assays for Anti-SARS-CoV-2 Antibody Detection." National Journal of Health Sciences 5, no. 4 (2021): 162–65. http://dx.doi.org/10.21089/njhs.54.0162.

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Abstract: Objective: To evaluate the diagnostic performance of Electrochemiluminescence (ECLIA) enzyme linked immunosorbent (ELISA) and lateral flow Immunofluorescence (LFIA) for anti-SARS-COV-2 antibody detection. Materials and Methods: Sensitivity was calculated with convalescent plasma (CP) donor’s samples. Specificity was checked by using pre-pandemic October 2019 samples. All samples were tested for anti-SARS-COV-2 antibody by using Electrochemiluminescence (ECLIA), Enzyme Linked Immunosorbent Assay (ELISA) and Lateral flow Immunofluorescence (LFIA) assay. Results: Total 55 patients were

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Wu, Mingyue. "Application of Enzyme-Linked Immunosorbent Assay in Early Diagnosis of Cancer." Highlights in Science, Engineering and Technology 99 (June 18, 2024): 73–78. http://dx.doi.org/10.54097/w0590f03.

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As far as the current development of the medical community is concerned, early detection is the most effective way to deal with cancer, while enzyme-linked immunosorbent assay (ELISA) has been introduced in several diagnosis area of cancer for a long time. This research summarizes the usage of ELISA in various sections from the process of cancer’s early detection and highlights its function in the process of cancer’s early detection. There are several types of ELISA, this research focuses on indirect ELISA and sandwich ELISA, representing their special abilities that assist to complete the pro

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Jiao, Lei, Lianhua Zhang, Wenwen Du, He Li, Dingyu Yang, and Chengzhou Zhu. "Au@Pt nanodendrites enhanced multimodal enzyme-linked immunosorbent assay." Nanoscale 11, no. 18 (2019): 8798–802. http://dx.doi.org/10.1039/c8nr08741e.

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Single modal enzyme-linked immunosorbent assay (ELISA) covering colorimetric, fluorescence, and chemiluminescence techniques has been widely reported in recent years, whereas the combination of multiple signal channels in one immunosensing platform still faces huge challenges.

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Jia, Qingyun, Hans-Uwe Dahms, and Lan Wang. "Detection of Metallothionein Proteins by Enzyme-Linked Immunosorbent Assay (ELISA)." Current Pharmaceutical Biotechnology 21, no. 7 (2020): 544–54. http://dx.doi.org/10.2174/1389201020666191127124629.

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Metallothioneins (MTs) are low-molecular-weight, cysteine-rich proteins that bind to heavy metals. MTs play a key role in the homeostasis of metal ions, maintaining intracellular redox equilibria and free radical scavenging. In several studies, under different conditions such as cancer development, drug therapy and heavy metal stress, the unique structural changes and functional effects of MT were studied. Although several assays are available to monitor the content and type of Metallothionein (MT) from environmental samples or in biomedical assays, Enzyme-Linked Immunosorbent Assays (ELISA) b

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Liu, Zhenjiang, Kewei Ren, Ming Li, Jiagao Wang, Jianfan Sun, and Daolin Du. "A new residue method for the determination of flonicamid in agricultural and environmental samples using enzyme immunoassay systems." RSC Advances 6, no. 42 (2016): 35842–46. http://dx.doi.org/10.1039/c5ra27425g.

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Sun, Jian, Xueping Ning, Lanyu Cui, Min Ling, Xiaoping Xu, and Shengbin He. "Assembly of “carrier free” enzymatic nano-reporters for improved ELISA." Analyst 145, no. 20 (2020): 6541–48. http://dx.doi.org/10.1039/d0an00585a.

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Zijlstra, E. E., N. S. Daifalla, P. A. Kager, et al. "rK39 Enzyme-Linked Immunosorbent Assay for Diagnosis of Leishmania donovani Infection." Clinical Diagnostic Laboratory Immunology 5, no. 5 (1998): 717–20. http://dx.doi.org/10.1128/cdli.5.5.717-720.1998.

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ABSTRACT The rK39 enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for Leishmania donovani infection in the Sudan. rK39 ELISA proved more sensitive than DAT in diagnosis of kala-azar (93 and 80%, respectively); both tests may remain positive up to 24 months after treatment. For patients with post-kala-azar dermal leishmaniasis and individuals with subclinical infection, rK39 ELISA performed as well as DAT but could detect infection 6 months earlier in ∼40% of patients. Conversion in DAT and rK39 ELISA also occurred in leishmanin skin test (LST)-po

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Chiu, Nan-Fu. "The Current Status and Future Promise of SPR Biosensors." Biosensors 12, no. 11 (2022): 933. http://dx.doi.org/10.3390/bios12110933.

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The most commonly used protein detection methods in clinical diagnosis and disease monitoring are enzyme-linked immunosorbent assay (ELISA), Western blotting (immunoblot), and lateral flow assay (LFA) rapid screening, of which ELISA is the gold standard immunoassay in clinical practice [...]

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Zeng, Kun, Yanmin Zou, Jianxia Liu, et al. "Enzyme-linked immunosorbent assay for triclocarban in aquatic environments." Water Science and Technology 72, no. 10 (2015): 1682–91. http://dx.doi.org/10.2166/wst.2015.366.

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A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of triclocarban (TCC) in waters and sediments. Haptens were synthesized by derivatizing the paraposition of a phenyl moiety of TCC. The synthesized hapten was then coupled to bovine thyroglobulin to be used as an immunogen, based on which, a high affinity monoclonal antibody 4D5 was produced with the hybridoma technique. Under the optimized conditions, using the monoclonal antibody, excellent performances of the assay were obtained: satisfactory sensitivity (IC50 (50% inhibition concentr

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Melgarejo, T., D. A. Williams та E. K. Asem. "Enzyme-linked immunosorbent assay for canine α1-protease inhibitor". American Journal of Veterinary Research 59, № 2 (1998): 127–30. http://dx.doi.org/10.2460/ajvr.1998.59.02.127.

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SUMMARY Objective To develop and validate an ELISA for quantifying α1-protease inhibitor (α1-PI) in serum and fecal extracts. Procedure Affinity-purified rabbit origin canine α1-PI antibodies were biotinylated and, after addition of streptavidin-horseradish peroxidase, were used as the labeled complex in a noncompetitive immunoassay. The α1-PI standards were made from purified serum canine α1-PI diluted in phosphate-buffered saline solution containing 5% newborn calf serum and 0.01% thimerosal. This assay was validated by determining linearity, recovery of added α1-PI, detection limit, and int

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Lee, Rachel C., Ru-Dong Wei, and Fun S. Chu. "Enzyme-Linked Immunosorbent Assay for T-2 Toxin Metabolites in Urine." Journal of AOAC INTERNATIONAL 72, no. 2 (1989): 345–48. http://dx.doi.org/10.1093/jaoac/72.2.345.

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Abstract A direct competitive enzyme-linked immunosorbent assay (ELISA) for determination of total T-2 toxin metabolites in urine was developed. The assay involves coating anti-3-acetyl-neosolaniol-hemisuccinate- bovine serum albumin conjugate (anti-3-Ac-NEOS-HS-BSA) antibody to the ELISA plate and using 3-Ac-NEOS-HS-peroxidase as the enzyme marker. Competitive ELISA revealed that the antibody had good cross-reactivity with acetyldiacetoxyscirpenol (Ac-DAS), T-2 tetraol tetraacetate, 3'-OH-Ac-T-2, 3-Ac-NEOS, and 3,4,15- triacetyl-12,13-epoxytrichothec-9-en-8-one (Ac-T-2-8-one), but less cross-

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Azimi, Nooshin T., Fujiang Wen, Richard M. Lister, Dennis A. Chen, and Fred E. Lytle. "Enzyme-Linked Immunoassays Using Nanosecond Fluorometric Detection." Applied Spectroscopy 46, no. 6 (1992): 994–98. http://dx.doi.org/10.1366/0003702924124402.

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Nanosecond temporal resolution is combined with an enzyme-linked immunosorbent assay (ELISA) to improve the lower limit of detection for a plant virus, brome mosaic virus. The method uses alkaline phosphatase as the enzyme link and β-naphthyl phosphate as the substrate. Enzymatic activity produces the highly fluorescent tag β-naphthol. The 8.9-ns lifetime of the tag allows temporal discrimination against the assay blank, providing a 64× improvement in the detection limit as compared to a steady-state measurement, and a ∼4100× improvement over a standard ELISA method incorporating the chromogen

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GARBER, ERIC A. E. "Detection of Melamine Using Commercial Enzyme-Linked Immunosorbent Assay Technology." Journal of Food Protection 71, no. 3 (2008): 590–94. http://dx.doi.org/10.4315/0362-028x-71.3.590.

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Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. To meet this need, commercial enzyme-linked immunosorbent assay (ELISA) test kits for the detection of triazines were evaluated. The recently released Melamine Plate kit (Abraxis, Warminster, Pa.) displayed a limit of detection of 9 ng/ml for melamine in phosphate-buffered saline (PBS) and approximately 1 μg/ml for melamine added to dog food. An atrazine ELISA test kit produced by Abraxis required 0.2 mg/ml to generate a response more than four times the standard deviation from background.

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Koppelman, Stef J., Gülsen Söylemez, Lynn Niemann, Ferdelie E. Gaskin, Joseph L. Baumert, and Steve L. Taylor. "Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/853836.

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Small amounts of sesame can trigger allergic reactions in sesame-allergic patients. Because sesame is a widely used food ingredient, analytical methods are needed to support quality control and food safety programs in the food industry. In this study, polyclonal antibodies against sesame seed proteins were raised, and an enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of sesame seed residue in food. A comparison was made between this ELISA and other assays, particularly focusing on recovery of sesame seed residue from different food matrices. The de

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Laze, Blerta, and Anila Mitre. "Comparison of ECL, ELISA and ELFA immunoassays." Buletini Shkencor Reald 8, no. 1 (2023): 40–49. http://dx.doi.org/10.59858/bshr100043.

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The aim of these study was the evaluation of electrochemiluminescence assay (ECL), enzyme-linked immunosorbent assay (ELISA) and enzyme-linked fluorescent assay (ELFA), for an early diagnosis of Cytomegalovirus infections in pregnant women. In medical diagnostics, it is necessary to determine the most sensitive techniques for the detection of this pathogen, due to it’s multiple fetal infections during pregnancy. The ECL technique resulted with the highest sensitivity and specificity values. However, for diagnostic purposes, the results should always be assessed in conjunction with the patient’

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Sheng, Enze, Mei Du, Jiachuan Yang, Xiude Hua, and Minghua Wang. "Development of immunoassays for detecting oxyfluorfen residue in agricultural and environmental samples." RSC Advances 8, no. 9 (2018): 5020–25. http://dx.doi.org/10.1039/c7ra12445g.

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