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Artigos de revistas sobre o tema "Spores and Biofilms"

Autor: Grafiati

Publicado: 22 de junho de 2024

Última modificação: 31 de julho de 2025

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1

Arnett, C. M., A. M. Beckman, M. D. Ginsberg, and V. F. Hock. "Influence of bacterial biofilms on Bacillus globigii spore viability in model chlorinated water distribution systems." Water Supply 10, no. 3 (2010): 277–85. http://dx.doi.org/10.2166/ws.2010.036.

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Viability of Bacillus globigii spores in chlorinated polyvinyl chloride (PVC) pipe loop systems were examined under oligotrophic conditions. Three 2.5 cm × 10 m pipe loops having poised free chlorine concentrations of 0.0, 0.5, and 1.0 mg/L were seeded with 3.0 × 108B. globigii spores each and viability was assessed over a 21 day period in both the recirculating waters and within the biofilms associated with pipe wall surfaces. After 10 min of exposure, viable spores were found to be associated within the pipe biofilms. In the untreated pipe loop spore counts remained statistically consistent

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Wijman, Janneke G. E., Patrick P. L. A. de Leeuw, Roy Moezelaar, Marcel H. Zwietering, and Tjakko Abee. "Air-Liquid Interface Biofilms of Bacillus cereus: Formation, Sporulation, and Dispersion." Applied and Environmental Microbiology 73, no. 5 (2007): 1481–88. http://dx.doi.org/10.1128/aem.01781-06.

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ABSTRACT Biofilm formation by Bacillus cereus was assessed using 56 strains of B. cereus, including the two sequenced strains, ATCC 14579 and ATCC 10987. Biofilm production in microtiter plates was found to be strongly dependent on incubation time, temperature, and medium, as well as the strain used, with some strains showing biofilm formation within 24 h and subsequent dispersion within the next 24 h. A selection of strains was used for quantitative analysis of biofilm formation on stainless steel coupons. Thick biofilms of B. cereus developed at the air-liquid interface, while the amount of

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3

LINDSAY, D., V. S. BRÖZEL, and A. von HOLY. "Spore Formation in Bacillus subtilis Biofilms." Journal of Food Protection 68, no. 4 (2005): 860–65. http://dx.doi.org/10.4315/0362-028x-68.4.860.

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Spore formation by a Bacillus strain (Bacillus subtilis SpoIVFB-GFP) engineered with a green fluorescent protein (GFP) fused to a polytopic membrane protein (SpoIVF) that fluoresces during sporulation was observed. Biofilms of B. subtilis SpoIVFB-GFP containing ca. 8 log CFU/ml vegetative cells and spores below the lower detection limit (i.e., &lt;1 log CFU/ml) were allowed to develop on glass wool (37°C). These biofilms were subsequently exposed to nutrient limitation to stimulate spore formation, which was monitored for fluorescence by confocal scanning laser microscopy. Sporulation in c

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Hu, Wen Si, Dong U. Woo, Yang Jae Kang, and Ok Kyung Koo. "Biofilm and Spore Formation of Clostridium perfringens and Its Resistance to Disinfectant and Oxidative Stress." Antibiotics 10, no. 4 (2021): 396. http://dx.doi.org/10.3390/antibiotics10040396.

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Clostridium perfringens is a major human pathogen that causes gastroenteritis via enterotoxin production and has the ability to form spores and biofilms for environmental persistence and disease transmission. This study aimed to compare the disinfectant and environmental resistance properties of C. perfringens vegetative cells and spores in planktonic and sessile conditions, and to examine the nucleotide polymorphisms and transcription under sessile conditions in C. perfringens strains isolated from meat. The sporulation rate of sessile C. perfringens TYJAM-D-66 (cpe+) was approximately 19% at

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5

LINDSAY, D., V. S. BRÖZEL, and A. VON HOLY. "Biofilm-Spore Response in Bacillus cereus and Bacillus subtilis during Nutrient Limitation." Journal of Food Protection 69, no. 5 (2006): 1168–72. http://dx.doi.org/10.4315/0362-028x-69.5.1168.

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This study aimed to trace the dynamics of biofilm formation by vegetative cells and endospores of Bacillus cereus DL5 and Bacillus subtilis 168. Counts of B. cereus DL5 and B. subtilis 168 vegetative cells and spores either attached to glass wool or, correspondingly, planktonic cells were determined by standard plate-counting methods. Results from this study highlighted the biofilm-forming potential of both spores and vegetative cells of two different Bacillus species. It was shown that once Bacillus spores had attached to a surface, the spores germinated under favorable (B. cereus DL5) and ev

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Hupka, Megan, Raj Kedia, Rylee Schauer, et al. "Morphology of Penicillium rubens Biofilms Formed in Space." Life 13, no. 4 (2023): 1001. http://dx.doi.org/10.3390/life13041001.

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Fungi biofilms have been found growing on spacecraft surfaces such as windows, piping, cables, etc. The contamination of these surfaces with fungi, although undesirable, is highly difficult to avoid. While several biofilm forming species, including Penicillium rubens, have been identified in spacecraft, the effect of microgravity on fungal biofilm formation is unknown. This study sent seven material surfaces (Stainless Steel 316, Aluminum Alloy, Titanium Alloy, Carbon Fiber, Quartz, Silicone, and Nanograss) inoculated with spores of P. rubens to the International Space Station and allowed biof

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Gong, Jinglei, Die Hu, Jinzhi He, Ling Zou, Zhu Chen, and Mingyun Li. "Effect of LongZhang Gargle on Dual-Species Biofilm of Candida albicans and Streptococcus mutans." BioMed Research International 2021 (March 22, 2021): 1–10. http://dx.doi.org/10.1155/2021/6654793.

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Bioactive natural products have become a hot spot for oral disease treatments. At the present study, LongZhang Gargle was investigated for its effects on single-species biofilms of Candida albicans and dual-species biofilms of Candida albicans and Streptococcus mutans. Two different models of single and dual-species biofilms were grown in YNBB medium under appropriate conditions. Biofilm biomass, biofilm architecture, and cell activity in biofilms were assessed using Crystal Violet Staining, MTT, scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM). Significant red

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Pizarro-Guajardo, Marjorie, Paulina Calderón-Romero, and Daniel Paredes-Sabja. "Ultrastructure Variability of the Exosporium Layer of Clostridium difficile Spores from Sporulating Cultures and Biofilms." Applied and Environmental Microbiology 82, no. 19 (2016): 5892–98. http://dx.doi.org/10.1128/aem.01463-16.

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ABSTRACTThe anaerobic sporeformerClostridium difficileis the leading cause of nosocomial antibiotic-associated diarrhea in developed and developing countries. The metabolically dormant spore form is considered the morphotype responsible for transmission, infection, and persistence, and the outermost exosporium layer is likely to play a major role in spore-host interactions during recurrent infections, contributing to the persistence of the spore in the host. A recent study (M. Pizarro-Guajardo, P. Calderón-Romero, P. Castro-Córdova, P. Mora-Uribe, and D. Paredes-Sabja, Appl Environ Microbiol 8

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ANJOS, MÁRCIA MARIA dos, SUELEN PEREIRA RUIZ, CELSO VATARU NAKAMURA, and BENÍCIO ALVES de ABREU FILHO. "Resistance of Alicyclobacillus acidoterrestris Spores and Biofilm to Industrial Sanitizers." Journal of Food Protection 76, no. 8 (2013): 1408–13. http://dx.doi.org/10.4315/0362-028x.jfp-13-020.

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This study evaluated the adhesion and biofilm formation of Alicyclobacillus acidoterrestris on industrial orange juice processing equipment and the bactericidal efficacy of peracetic acid, sodium hypochlorite, and quaternary ammonia after biofilm formation. The efficacy of these sanitizers against the spores of this microorganism was also evaluated. Stainless steel and nylon surfaces exhibited higher cell adhesion levels than did polyvinyl chloride surfaces. Peracetic acid was the most effective in removing biofilms from all surfaces (P &lt; 0.05) and also reduced bacterial counts by 3 log

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Gerwig, Jan, Taryn B. Kiley, Katrin Gunka, Nicola Stanley-Wall, and Jörg Stülke. "The protein tyrosine kinases EpsB and PtkA differentially affect biofilm formation in Bacillus subtilis." Microbiology 160, no. 4 (2014): 682–91. http://dx.doi.org/10.1099/mic.0.074971-0.

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The Gram-positive soil bacterium Bacillus subtilis is able to choose between motile and sessile lifestyles. The sessile way of life, also referred to as biofilm, depends on the formation of an extracellular polysaccharide matrix and some extracellular proteins. Moreover, a significant proportion of cells in a biofilm form spores. The first two genes of the 15-gene operon for extracellular polysaccharide synthesis, epsA and epsB, encode a putative transmembrane modulator protein and a putative protein tyrosine kinase, respectively, with similarity to the TkmA/PtkA modulator/kinase couple. Here

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Singh, Rachna, M. R. Shivaprakash, and Arunaloke Chakrabarti. "Biofilm formation by zygomycetes: quantification, structure and matrix composition." Microbiology 157, no. 9 (2011): 2611–18. http://dx.doi.org/10.1099/mic.0.048504-0.

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Most studies on fungal biofilms have focused on Candida in yeasts and Aspergillus in mycelial fungi. To the authors’ knowledge, biofilm formation by zygomycetes has not been reported previously. In this study, the biofilm-forming capacity of Rhizopus oryzae, Lichtheimia corymbifera, Rhizomucor pusillus and Apophysomyces elegans was evaluated. At appropriate seeding spore densities, Rhp . oryzae (105 c.f.u. ml−1), L. corymbifera (104 c.f.u. ml−1) and Rhm. pusillus (104 c.f.u. ml−1) produced highly intertwined, adherent structures on flat-bottomed polystyrene microtitre plates after 24 h at 37 °

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12

Veening, Jan-Willem, Oscar P. Kuipers, Stanley Brul, Klaas J. Hellingwerf, and Remco Kort. "Effects of Phosphorelay Perturbations on Architecture, Sporulation, and Spore Resistance in Biofilms of Bacillus subtilis." Journal of Bacteriology 188, no. 8 (2006): 3099–109. http://dx.doi.org/10.1128/jb.188.8.3099-3109.2006.

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ABSTRACT The spore-forming bacterium Bacillus subtilis is able to form highly organized multicellular communities called biofilms. This coordinated bacterial behavior is often lost in domesticated or laboratory strains as a result of planktonic growth in rich media for many generations. However, we show here that the laboratory strain B. subtilis 168 is still capable of forming spatially organized multicellular communities on minimal medium agar plates, exemplified by colonies with vein-like structures formed by elevated bundles of cells. In line with the current model for biofilm formation, w

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13

Zhao, Yu, Martien P. M. Caspers, Karin I. Metselaar, et al. "Abiotic and Microbiotic Factors Controlling Biofilm Formation by Thermophilic Sporeformers." Applied and Environmental Microbiology 79, no. 18 (2013): 5652–60. http://dx.doi.org/10.1128/aem.00949-13.

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ABSTRACTOne of the major concerns in the production of dairy concentrates is the risk of contamination by heat-resistant spores from thermophilic bacteria. In order to acquire more insight in the composition of microbial communities occurring in the dairy concentrate industry, a bar-coded 16S amplicon sequencing analysis was carried out on milk, final products, and fouling samples taken from dairy concentrate production lines. The analysis of these samples revealed the presence of DNA from a broad range of bacterial taxa, including a majority of mesophiles and a minority of (thermophilic) spor

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14

Burgess, S. A., J. D. Brooks, J. Rakonjac, K. M. Walker, and S. H. Flint. "The formation of spores in biofilms ofAnoxybacillus flavithermus." Journal of Applied Microbiology 107, no. 3 (2009): 1012–18. http://dx.doi.org/10.1111/j.1365-2672.2009.04282.x.

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15

KRESKE, AUDREY C., JEE-HOON RYU, CHARLES A. PETTIGREW, and LARRY R. BEUCHAT. "Lethality of Chlorine, Chlorine Dioxide, and a Commercial Produce Sanitizer to Bacillus cereus and Pseudomonas in a Liquid Detergent, on Stainless Steel, and in Biofilm." Journal of Food Protection 69, no. 11 (2006): 2621–34. http://dx.doi.org/10.4315/0362-028x-69.11.2621.

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Many factors that are not fully understood may influence the effectiveness of sanitizer treatments for eliminating pathogens and spoilage microorganisms in food or detergent residues or in biofilms on food contact surfaces. This study was done to determine the sensitivities of Pseudomonas cells and Bacillus cereus cells and spores suspended in a liquid dishwashing detergent and inoculated onto the surface of stainless steel to treatment with chlorine, chlorine dioxide, and a commercial produce sanitizer (Fit). Cells and spores were incubated in a liquid dishwashing detergent for 16 to 18 h bef

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16

KİLİC, TUGBA. "Biofilm-Forming Ability and Effect of Sanitation Agents on Biofilm-Control of Thermophile Geobacillus sp. D413 and Geobacillus toebii E134." Polish Journal of Microbiology 69, no. 4 (2020): 411–19. http://dx.doi.org/10.33073/pjm-2020-042.

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Geobacillus sp. D413 and Geobacillus toebii E134 are aerobic, non-pathogenic, endospore-forming, obligately thermophilic bacilli. Gram-positive thermophilic bacilli can produce heat-resistant spores. The bacteria are indicator organisms for assessing the manufacturing process’s hygiene and are capable of forming biofilms on surfaces used in industrial sectors. The present study aimed to determine the biofilm-forming properties of Geobacillus isolates and how to eliminate this formation with sanitation agents. According to the results, extracellular DNA (eDNA) was interestingly not affected by

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Dobretsov, Sergey, and Daniel Rittschof. "Love at First Taste: Induction of Larval Settlement by Marine Microbes." International Journal of Molecular Sciences 21, no. 3 (2020): 731. http://dx.doi.org/10.3390/ijms21030731.

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Marine biofilms are composed of many species of bacteria, unicellular algae, and protozoa. Biofilms can induce, inhibit, or have no effect on settlement of larvae and spores of algae. In this review, we focus on induction of larval settlement by marine bacteria and unicellular eukaryotes and review publications from 2010 to September 2019. This review provides insights from meta-analysis on what is known about the effect of marine biofilms on larval settlement. Of great interest is the impact of different components of marine biofilms, such as bacteria and diatoms, extracellular polymeric subs

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18

Tam, Nguyen K. M., Nguyen Q. Uyen, Huynh A. Hong, et al. "The Intestinal Life Cycle of Bacillus subtilis and Close Relatives." Journal of Bacteriology 188, no. 7 (2006): 2692–700. http://dx.doi.org/10.1128/jb.188.7.2692-2700.2006.

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ABSTRACT Bacillus subtilis is considered a soil organism for which endospore formation provides a means to ensure long-term survival in the environment. We have addressed here the question of what happens to a spore when ingested. Spores displaying on their surface a heterologous antigen, tetanus toxin fragment C (TTFC), were shown to generate anti-TTFC responses not to the antigen contained in the primary oral inoculum but to those displayed on spores that had germinated and then resporulated. We then used reverse transcription-PCR to determine expression of vegetative genes and sporulation-s

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Huang, Yiying, Steve H. Flint, and Jon S. Palmer. "Bacillus cereus spores and toxins – The potential role of biofilms." Food Microbiology 90 (September 2020): 103493. http://dx.doi.org/10.1016/j.fm.2020.103493.

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K, Capriotti, Pelletier J, Barone S, and Capriotti J. "Efficacy of Dilute Povidone-Iodine against Multi- Drug Resistant Bacterial Biofilms, Fungal Biofilms and Fungal Spores." Journal of Clinical Research in Dermatology 5, no. 1 (2018): 1–5. http://dx.doi.org/10.15226/2378-1726/5/1/00174.

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Wang, Kai-Ling, Zheng-Rong Dou, Gao-Fen Gong, Hai-Feng Li, Bei Jiang, and Ying Xu. "Anti-Larval and Anti-Algal Natural Products from Marine Microorganisms as Sources of Anti-Biofilm Agents." Marine Drugs 20, no. 2 (2022): 90. http://dx.doi.org/10.3390/md20020090.

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Bacteria growing inside biofilms are more resistant to hostile environments, conventional antibiotics, and mechanical stresses than their planktonic counterparts. It is estimated that more than 80% of microbial infections in human patients are biofilm-based, and biofouling induced by the biofilms of some bacteria causes serious ecological and economic problems throughout the world. Therefore, exploring highly effective anti-biofilm compounds has become an urgent demand for the medical and marine industries. Marine microorganisms, a well-documented and prolific source of natural products, provi

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Permpoonpattana, P., H. A. Hong, R. Khaneja, and S. M. Cutting. "Evaluation of Bacillus subtilis strains as probiotics and their potential as a food ingredient." Beneficial Microbes 3, no. 2 (2012): 127–35. http://dx.doi.org/10.3920/bm2012.0002.

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Spores of Bacillus subtilis including one strain used commercially were evaluated for their potential value as a probiotic and as potential food additives. Two isolates of B. subtilis examined here were HU58, a human isolate and PXN21, a strain used in an existing commercial product. Compared to a domesticated laboratory strain of B. subtilis both isolates carried traits that could prove advantageous in the human gastro-intestinal tract. This included full resistance to gastric fluids, rapid sporulation and the formation of robust biofilms. We also showed that PXN21 spores when administered we

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Puttaiah, Raghunath, Kathy KH Svoboda, Shih Ming Lin, et al. "Evaluation of an Automated Dental Unit Water System's Contamination Control Protocol." Journal of Contemporary Dental Practice 13, no. 1 (2012): 1–10. http://dx.doi.org/10.5005/jp-journals-10024-1087.

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ABSTRACT Background This study addresses the efficacy of an automated decontamination protocol using the germicide ‘tetra acetyl ethylene diamine (TAED) perborate’ (Farmec SpA, Italy). The germicide TAED perborate protocol is used in the Castellini Dental Units fitted with an Autosteril unit (an automated device that can cycle 0.26% TAED perborate solution and sterile water for cleaning the water system between patients and overnight). Prior to testing the Autosteril and the 0.26% TAED perborate protocol on the Logos Jr Dental Unit (Castellini SpA, Italy), TAED perborate was used on a dental u

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Holzman, David C. "Agent from Soil Microbe Blocks B. subtilis from Forming Biofilms, Spores." Microbe Magazine 10, no. 6 (2015): 230. http://dx.doi.org/10.1128/microbe.10.230.1.

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Doggett, M. Steven. "Characterization of Fungal Biofilms within a Municipal Water Distribution System." Applied and Environmental Microbiology 66, no. 3 (2000): 1249–51. http://dx.doi.org/10.1128/aem.66.3.1249-1251.2000.

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ABSTRACT Biofilms of a municipal water distribution system were characterized to assess the occurrence of fungi within surface matrixes. Densities of filamentous fungi ranged from 4.0 to 25.2 CFU cm−2, whereas yeast densities ranged from 0 to 8.9 CFU cm−2. Observations by scanning electron microscopy further suggested that spores, not hyphae or vegetative cells, comprised the primary source of viable propagules.

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Xaplanteri, Panagiota, Chrysanthi Oikonomopoulou, Chrysanthi Xini, and Charalampos Potsios. "Community-Acquired Clostridioides difficile Infection: The Fox Among the Chickens." International Journal of Molecular Sciences 26, no. 10 (2025): 4716. https://doi.org/10.3390/ijms26104716.

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Clostridioides difficile infection (CDI) appears mainly as nosocomial antibiotic-associated diarrhea, and community-acquired infection is increasingly being recognized. The threshold of asymptomatic colonization and the clinical manifestation of CDI need further elucidation. Community-acquired CDI (CA-CDI) should be considered when the disease commences within 48 h of admission to hospital or more than 12 weeks after discharge. Although CDI is not established as a food-borne or zoonotic disease, some data support that direction. The spores’ ability to survive standard cooking procedures and on

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Houry, A., R. Briandet, S. Aymerich, and M. Gohar. "Involvement of motility and flagella in Bacillus cereus biofilm formation." Microbiology 156, no. 4 (2010): 1009–18. http://dx.doi.org/10.1099/mic.0.034827-0.

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Bacillus cereus is a food-borne pathogen and a frequent contaminant of food production plants. The persistence of this pathogen in various environments results from the formation of spores and of biofilms. To investigate the role of the B. cereus flagellar apparatus in biofilm formation, we constructed a non-flagellated mutant and a flagellated but non-motile mutant. Unexpectedly, we found that the presence of flagella decreased the adhesion of the bacterium to glass surfaces. We hypothesize that this decrease is a consequence of the flagella hindering a direct interaction between the bacteria

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Park, Hyong Seok, Jungwoo Yang, Hee Jung Choi, and Kyoung Heon Kim. "Effective Thermal Inactivation of the Spores of Bacillus cereus Biofilms Using Microwave." Journal of Microbiology and Biotechnology 27, no. 7 (2017): 1209–15. http://dx.doi.org/10.4014/jmb.1702.02009.

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Pirog, T. P., L. V. Kliuchka, T. A. Shevchuk, and G. O. Iutynska. "Destruction of Biofilms on Silicone Tubes under the Action of a Mixture of Nocardia vaccinii IMV B-7405 Surfactants with other Biocides." Mikrobiolohichnyi Zhurnal 83, no. 4 (2021): 43–53. http://dx.doi.org/10.15407/microbiolj83.04.043.

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The formation of pathogenic microorganisms biofilms on the central venous catheter is the cause of catheter-associated infections. An alternative method of combating biofilms is the use of “antibacterial” and “antifungal” locks, which are solutions of antibiotics or antifungal drugs in a mixture with other natural compounds, which can be microbial surface-active substances (surfactants) or essential oils. Aim. To investigate the role of Nocardia vaccinii IMV B-7405 surfactants mixture with other antimicrobial compounds in the destruction of biofilm on silicone tubes. Methods. N. vaccinii IMV B

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Tirloni, Erica, Simone Stella, Francesco Celandroni, Diletta Mazzantini, Cristian Bernardi, and Emilia Ghelardi. "Bacillus cereus in Dairy Products and Production Plants." Foods 11, no. 17 (2022): 2572. http://dx.doi.org/10.3390/foods11172572.

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Spore-forming Bacillus cereus is a common contaminant of dairy products. As the microorganism is widespread in the environment, it can contaminate milk at the time of milking, but it can also reach the dairy products in each phase of production, storage and ripening. Milk pasteurization treatment is not effective in reducing contamination and can instead act as an activator of spore germination, and a potential associated risk still exists with the consumption of some processed foods. Prevalences and concentrations of B. cereus in milk and dairy products are extremely variable worldwide: in pa

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Hoque, Enamul, Stephan Pflugmacher, Johannes Fritscher, and Manfred Wolf. "Induction of Glutathione S-Transferase in Biofilms and Germinating Spores of Mucor hiemalis Strain EH5 from Cold Sulfidic Spring Waters." Applied and Environmental Microbiology 73, no. 8 (2007): 2697–707. http://dx.doi.org/10.1128/aem.02786-06.

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ABSTRACT The occurrence and activation of glutathione S-transferase (GST) and the GST activities in biofilms in cold sulfidic spring waters were compared to the occurrence and activation of GST and the GST activities of the aquatic fungal strains EH5 and EH7 of Mucor hiemalis isolated for the first time from such waters. Using fluorescently labeled polyclonal anti-GST antibodies and GST activity measurements, we demonstrated that a high level of GST occurred in situ in natural biofilms and pure cultures of strain EH5. Measurement of microsomal and cytosolic soluble GST activities using differe

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SVENSSON, BIRGITTA, ÅSA ENEROTH, JOHANNE BRENDEHAUG, GÖRAN MOLIN, and ANDERS CHRISTIANSSON. "Involvement of a pasteurizer in the contamination of milk by Bacillus cereus in a commercial dairy plant." Journal of Dairy Research 67, no. 3 (2000): 455–60. http://dx.doi.org/10.1017/s0022029900004313.

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Bacillus cereus is a common contaminant in raw milk. The spores survive pasteurization and psychrotrophic strains of B. cereus often limit the keeping quality of pasteurized milk stored at > 6 °C (Griffiths, 1992). High numbers of B. cereus in pasteurized milk are most frequent when the cows are grazing (Slaghuis et al. 1997), mainly owing to increased levels of spores in raw milk resulting from teat contamination by soil (Christiansson et al. 1999). However, high numbers can also be found in pasteurized milk while the cows are housed indoors, and this is probably caused by additional conta

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Hosni, Ahmed A., Jeffery G. Szabo, and Paul L. Bishop. "Efficacy of Chlorine Dioxide as a Disinfectant for Bacillus Spores in Drinking-Water Biofilms." Journal of Environmental Engineering 137, no. 7 (2011): 569–74. http://dx.doi.org/10.1061/(asce)ee.1943-7870.0000355.

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Rabochová, M., M. Kulišová, J. Lorinčík, et al. "Assessing the antimicrobial efficacy of nonthermal plasma treatment on filamentous fungi spores and biofilms." Food Control 163 (September 2024): 110522. http://dx.doi.org/10.1016/j.foodcont.2024.110522.

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Giglio, Krista M., Chengjun Zhu, Courtney Klunder, Shelley Kummer, and Anthony G. Garza. "The Enhancer Binding Protein Nla6 Regulates Developmental Genes That Are Important for Myxococcus xanthus Sporulation." Journal of Bacteriology 197, no. 7 (2015): 1276–87. http://dx.doi.org/10.1128/jb.02408-14.

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ABSTRACTIn the bacteriumMyxococcus xanthus, starvation triggers the formation of multicellular fruiting bodies containing thousands of stress-resistant spores. Recent work showed that fruiting body development is regulated by a cascade of transcriptional activators called enhancer binding proteins (EBPs). The EBP Nla6 is a key component of this cascade; it regulates the promoters of other EBP genes, including a downstream-functioning EBP gene that is crucial for sporulation. In recent expression studies, hundreds of Nla6-dependent genes were identified, suggesting that the EBP gene targets of

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Tsarelunga, А. А., and Е. Yu Blagoveschenskaya. "Phylloplane as fungi habitat." Журнал общей биологии 84, no. 4 (2023): 263–78. http://dx.doi.org/10.31857/s0044459623040073.

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As currently shown the phylloplane of different plants is actively colonized by yeasts and filamentous fungi of different taxonomic groups. The features of the leaf as a microhabitat are low humidity, susceptibility to mechanical effects of rain and wind, lack of nutrients on the surface, and high insolation, which causes the allocation of epiphytic fungi as a separate ecological group. Although the data vary from plant to plant, in general it can be said that basidial yeasts and such filamentous fungi as Alternaria, Epicoccum, Cladosporium, Phoma and Trichoderma are most commonly found on pla

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Schuerger, Andrew C., Steven Trigwell, and Carlos I. Calle. "Use of non-thermal atmospheric plasmas to reduce the viability ofBacillus subtilison spacecraft surfaces." International Journal of Astrobiology 7, no. 1 (2008): 47–57. http://dx.doi.org/10.1017/s1473550407004016.

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AbstractAtmospheric pressure glow-discharge (APGD) plasmas have been proposed for sterilizing spacecraft surfaces prior to launch. The advantages of APGD plasmas for the sterilization of spacecraft surfaces include low temperatures at treatment sites, rapid inactivation kinetics of exposed microbial cells, physical degradation and removal of microbial cells, physical removal of organic biosignature molecules, and short exposure times for the materials. However, few studies have tested APGD plasmas on spacecraft materials for their effectiveness in both sterilizing surfaces and removal of micro

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Mowat, Eilidh, John Butcher, Sue Lang, Craig Williams, and Gordon Ramage. "Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus." Journal of Medical Microbiology 56, no. 9 (2007): 1205–12. http://dx.doi.org/10.1099/jmm.0.47247-0.

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Aspergillus fumigatus is an increasingly prevalent opportunistic fungal pathogen of various immunocompromised individuals. It has the ability to form filaments within the lungs, producing dense intertwined mycelial balls, which are difficult to treat. The aim of this study was to develop a suitable model of A. fumigatus to examine the effects of antifungal challenge on these intertwined filamentous communities. A. fumigatus NCPF 7367 growth conditions were optimized on both Thermanox coverslips and on flat-bottomed microtitre plates to establish optimal conidial seeding densities. Isolates wer

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Engel, Tobias, Paul E. Verweij, Joost van den Heuvel, et al. "Parasexual recombination enables Aspergillus fumigatus to persist in cystic fibrosis." ERJ Open Research 6, no. 4 (2020): 00020–2020. http://dx.doi.org/10.1183/23120541.00020-2020.

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Aspergillus fumigatus is a saprobic fungus that causes a range of pulmonary diseases, some of which are characterised by fungal persistence such as is observed in cystic fibrosis (CF) patients. Creation of genetic variation is critical for A. fumigatus to adapt to the lung environment, but biofilm formation, especially in CF patients, may preclude mutational supply in A. fumigatus due to its confinement to the hyphal morphotype. We tested our hypothesis that genetic variation is created through parasexual recombination in chronic biofilms by phenotypic and genetic analysis of A. fumigatus isol

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Mohsin, Muhammad Zubair, Rabia Omer, Jiaofang Huang, et al. "Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials." Synthetic and Systems Biotechnology 6, no. 3 (2021): 180–91. http://dx.doi.org/10.1016/j.synbio.2021.07.002.

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AXELSSON, L., A. HOLCK, I. RUD, et al. "Cleaning of Conveyor Belt Materials Using Ultrasound in a Thin Layer of Water." Journal of Food Protection 76, no. 8 (2013): 1401–7. http://dx.doi.org/10.4315/0362-028x.jfp-12-563.

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Cleaning of conveyor belts in the food industry is imperative for preventing the buildup of microorganisms that can contaminate food. New technologies for decreasing water and energy consumption of cleaning systems are desired. Ultrasound can be used for cleaning a wide range of materials. Most commonly, baths containing fairly large amounts of water are used. One possibility to reduce water consumption is to use ultrasonic cavitation in a thin water film on a flat surface, like a conveyor belt. In order to test this possibility, a model system was set up, consisting of an ultrasound transduce

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Khan, Mahjabeen, Murray McDonald, Kaustubh Mundada, and Mark Willcox. "Efficacy of Ultraviolet Radiations against Coronavirus, Bacteria, Fungi, Fungal Spores and Biofilm." Hygiene 2, no. 3 (2022): 120–31. http://dx.doi.org/10.3390/hygiene2030010.

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Ultra-violet (UV) C (200–280 wavelength) light has long been known for its antimicrobial and disinfecting efficacy. It damages DNA by causing the dimerization of pyrimidines. A newly designed technology (MUVi-UVC; Mobile UV Innovations Pty Ltd., Melbourne, VIC, Australia) that emits UVC at 240 nm is composed of an enclosed booth with three UVC light stands each with four bulbs, and has been developed for disinfecting mobile medical equipment. The aim of this project was to examine the spectrum of antimicrobial activity of this device. The experiments were designed following ASTM E1052-20, EN14

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Huang, Yiying, Steve H. Flint, and Jon S. Palmer. "The heat resistance of spores from biofilms of Bacillus cereus grown in tryptic soy broth and milk." International Dairy Journal 123 (December 2021): 105169. http://dx.doi.org/10.1016/j.idairyj.2021.105169.

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Koval’ová, Zuzana, Kataŕna Tarabová, Karol Hensel, and Zdenko Machala. "Decontamination of Streptococci biofilms and Bacillus cereus spores on plastic surfaces with DC and pulsed corona discharges." European Physical Journal Applied Physics 61, no. 2 (2013): 24306. http://dx.doi.org/10.1051/epjap/2012120449.

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Prado, Daniela Biral do, Márcia Maria dos Anjos Szczerepa, Otávio Augusto Capeloto, et al. "Effect of ultraviolet (UV-C) radiation on spores and biofilms of Alicyclobacillus spp. in industrialized orange juice." International Journal of Food Microbiology 305 (September 2019): 108238. http://dx.doi.org/10.1016/j.ijfoodmicro.2019.108238.

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Akishev, Yuri, Michail Grushin, Vladimir Karalnik, et al. "Atmospheric-pressure, nonthermal plasma sterilization of microorganisms in liquids and on surfaces." Pure and Applied Chemistry 80, no. 9 (2008): 1953–69. http://dx.doi.org/10.1351/pac200880091953.

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Gas discharge plasma inactivation of microorganisms at low (close to ambient) temperature is a promising area of investigation that is attracting widespread interest. This paper describes atmospheric-pressure, nonthermal plasma (NTP) methods for cold sterilization of liquids and thermal sensitive surfaces. These methods are based on the use of direct current (DC) gas discharge plasma sources fed with steady-state high voltage. Parameters characterizing the plasma sources used (plasma-forming gas, gas flow rate, electric power consumed, etc.) are given. The results for plasma sterilization of d

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Gómez-Pérez, Daniel, Vasvi Chaudhry, Ariane Kemen, and Eric Kemen. "Amyloid Proteins in Plant-Associated Microbial Communities." Microbial Physiology 31, no. 2 (2021): 88–98. http://dx.doi.org/10.1159/000516014.

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Amyloids have proven to be a widespread phenomenon rather than an exception. Many proteins presenting the hallmarks of this characteristic beta sheet-rich folding have been described to date. Particularly common are functional amyloids that play an important role in the promotion of survival and pathogenicity in prokaryotes. Here, we describe important developments in amyloid protein research that relate to microbe-microbe and microbe-host interactions in the plant microbiome. Starting with biofilms, which are a broad strategy for bacterial persistence that is extremely important for plant col

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KOSEKI, SHIGENOBU, KYOICHIRO YOSHIDA, SEIICHIRO ISOBE, and KAZUHIKO ITOH. "Decontamination of Lettuce Using Acidic Electrolyzed Water." Journal of Food Protection 64, no. 5 (2001): 652–58. http://dx.doi.org/10.4315/0362-028x-64.5.652.

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The disinfectant effect of acidic electrolyzed water (AcEW), ozonated water, and sodium hypochlorite (NaOCl) solution on lettuce was examined. AcEW (pH 2.6; oxidation reduction potential, 1140 mV; 30 ppm of available chlorine) and NaOCl solution (150 ppm of available chlorine) reduced viable aerobes in lettuce by 2 log CFU/g within 10 min. For lettuce washed in alkaline electrolyzed water (AlEW) for 1 min and then disinfected in AcEW for 1 min, viable aerobes were reduced by 2 log CFU/g. On the other hand, ozonated water containing 5 ppm of ozone reduced viable aerobes in lettuce 1.5 log CFU/g

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Syrchina, N. V., L. V. Pilip, E. P. Kolevatykh, and T. Ya Ashikhmina. "Effect of various processing methods on the numbers of Clostridium (Clostridia, Bacteria) in animal by-products." Povolzhskiy Journal of Ecology, no. 4 (December 12, 2023): 466–80. http://dx.doi.org/10.35885/1684-7318-2023-4-466-480.

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Clostridia are one of the largest genera of eubacteria living in water, soil, the intestines of humans and animals. The genus Clostridium includes both pathogenic and non-pathogenic species. Representatives of this genus occupy a leading position among all types of bacteria in producing a variety of toxins. Due to their ability to form spores, these microorganisms can survive in the most unfavorable conditions. Antibiotic-resistant Clostridia, which are released to the environment as part of farm animal manure, are of particular environmental danger. We have studied the influence of various tr

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Rao, Dhana, Jeremy S. Webb, Carola Holmström, et al. "Low Densities of Epiphytic Bacteria from the Marine Alga Ulva australis Inhibit Settlement of Fouling Organisms." Applied and Environmental Microbiology 73, no. 24 (2007): 7844–52. http://dx.doi.org/10.1128/aem.01543-07.

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ABSTRACT Bacteria that produce inhibitory compounds on the surface of marine algae are thought to contribute to the defense of the host plant against colonization of fouling organisms. However, the number of bacterial cells necessary to defend against fouling on the plant surface is not known. Pseudoalteromonas tunicata and Phaeobacter sp. strain 2.10 (formerly Roseobacter gallaeciensis) are marine bacteria often found in association with the alga Ulva australis and produce a range of extracellular inhibitory compounds against common fouling organisms. P. tunicata and Phaeobacter sp. strain 2.

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