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Artigos de revistas sobre o tema "Tissue"

Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 5 de abril de 2025

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1

Schmidt, Christine E., e Jennie M. Baier. "Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering". Biomaterials 21, n.º 22 (novembro de 2000): 2215–31. http://dx.doi.org/10.1016/s0142-9612(00)00148-4.

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2

Kishida, Akio, Seiichi Funamoto, Jun Negishi, Yoshihide Hashimoto, Kwangoo Nam, Tsuyoshi Kimura, Toshiya Fujisato e Hisatoshi Kobayashi. "Tissue Engineering with Natural Tissue Matrices". Advances in Science and Technology 76 (outubro de 2010): 125–32. http://dx.doi.org/10.4028/www.scientific.net/ast.76.125.

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Natural tissue, especially autologous tissue is one of ideal materials for tissue regeneration. Decellularized tissue could be assumed as a second choice because the structure and the mechanical properties are well maintained. Decellularized human tissues, for instance, heart valve, blood vessel, and corium, have already been developed and applied clinically. Nowadays, decellularized porcine tissues are also investigated. These decellularized tissues were prepared by detergent treatment. The detergent washing is easy but sometime it has problems. We have developed the novel decellularization method, which applied the high-hydrostatic pressure (HHP). As the tissue set in the pressurizing chamber is treated uniformly, the effect of the high-hydrostatic pressurization does not depend on the size of tissue. We have reported the HHP decellularization of heart valve, blood vessel, bone, and cornea. Furthermore, HHP treatments are reported to have the ability of the extinction of bacillus and the inactivation of virus. So, the HHP treatment is also expected as the sterilization method. We are investigating efficient processes of decellularization and recellularization of biological tissues to have bioscaffolds keeping intact structure and biomechanical properties. Our recent studies on tissue engineering using HHP decellularized tissue will be reported here.

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3

Okano, T. "Muscular tissue engineering: capillary-incorporated hybrid muscular tissues in vivo tissue culture". Cell Transplantation 7, n.º 5 (10 de setembro de 1998): 435–42. http://dx.doi.org/10.1016/s0963-6897(98)00030-x.

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Okano, Takahisa, e Takehisa Matsuda. "Muscular Tissue Engineering: Capillary-Incorporated Hybrid Muscular Tissues in Vivo Tissue Culture". Cell Transplantation 7, n.º 5 (setembro de 1998): 435–42. http://dx.doi.org/10.1177/096368979800700502.

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Requirements for a functional hybrid muscular tissue are 1) a high density of multinucleated cells, 2) a high degree of cellular orientation, and 3) the presence of a capillary network in the hybrid tissue. Rod-shaped hybrid muscular tissues composed of C2C12 cells (skeletal muscle myoblast cell line) and type I collagen, which were prepared using the centrifugal cell-packing method reported in our previous article, were implanted into nude mice. The grafts, comprised three hybrid tissues (each dimension, diameter, approximately 0.3 mm, length, approximately 1 mm, respectively), were inserted into the subcutaneous spaces on the backs of nude mice. All nude mice that survived the implantation were sacrificed at 1, 2, and 4 wk after the implantation. The grafts were easily distinguishable from the subcutaneous tissues of host mice with implantation time. The grafts increased in size with time after implantation, and capillary networks were formed in the vicinities and on the surfaces of the grafts. One week after implantation, many capillaries formed in the vicinities of the grafts. In the central portion of the graft, few capillaries and necrotic cells were observed. Mononucleated myoblasts were densely distributed and a low number of multinucleated myotubes were scattered. Two weeks after implantation, the formation of a capillary network was induced, resulting in the surfaces of the grafts being covered by capillaries. Numerous elongated multinucleated myotubes and mononucleated myoblasts were densely distributed and numerous capillaries were observed throughout the grafts. Four weeks after implantation a dense capillary network was formed in the vicinities and on the surfaces of the grafts. In the peripheral portion of the graft, multinucleated myotubes in the vicinities of the rich capillaries were observed. Thus, hybrid muscular tissues in vitro preconstructed was remodeled in vivo, which resulted in facilitating the incorporation of capillary networks into the tissues. © 1998 Elsevier Science Inc.

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Criddle, Richard S., Lee D. Hansen, Brian F. Woodfield e H. Dennis Tolley. "Modeling transthyretin (TTR) amyloid diseases, from monomer to amyloid fibrils". PLOS ONE 19, n.º 6 (6 de junho de 2024): e0304891. http://dx.doi.org/10.1371/journal.pone.0304891.

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ATTR amyloidosis is caused by deposition of large, insoluble aggregates (amyloid fibrils) of cross-β-sheet TTR protein molecules on the intercellular surfaces of tissues. The process of amyloid formation from monomeric TTR protein molecules to amyloid deposits has not been fully characterized and is therefore modeled in this paper. Two models are considered: 1) TTR monomers in the blood spontaneously fold into a β-sheet conformation, aggregate into short proto-fibrils that then circulate in the blood until they find a complementary tissue where the proto-fibrils accumulate to form the large, insoluble amyloid fibrils found in affected tissues. 2) TTR monomers in the native or β-sheet conformation circulate in the blood until they find a tissue binding site and deposit in the tissue or tissues forming amyloid deposits in situ. These models only differ on where the selection for β-sheet complementarity occurs, in the blood where wt-wt, wt-v, and v-v interactions determine selectivity, or on the tissue surface where tissue-wt and tissure-v interactions also determine selectivity. Statistical modeling in both cases thus involves selectivity in fibril aggregation and tissue binding. Because binding of protein molecules into fibrils and binding of fibrils to tissues occurs through multiple weak non-covalent bonds, strong complementarity between β-sheet molecules and between fibrils and tissues is required to explain the insolubility and tissue selectivity of ATTR amyloidosis. Observation of differing tissue selectivity and thence disease phenotypes from either pure wildtype TTR protein or a mix of wildtype and variant molecules in amyloid fibrils evidences the requirement for fibril-tissue complementarity. Understanding the process that forms fibrils and binds fibrils to tissues may lead to new possibilities for interrupting the process and preventing or curing ATTR amyloidosis.

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Bakhshandeh, Behnaz, Payam Zarrintaj, Mohammad Omid Oftadeh, Farid Keramati, Hamideh Fouladiha, Salma Sohrabi-jahromi e Zarrintaj Ziraksaz. "Tissue engineering; strategies, tissues, and biomaterials". Biotechnology and Genetic Engineering Reviews 33, n.º 2 (3 de julho de 2017): 144–72. http://dx.doi.org/10.1080/02648725.2018.1430464.

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7

Hardingham, Tim. "Tissue engineering: Designing for health". Biochemist 25, n.º 5 (1 de outubro de 2003): 19–21. http://dx.doi.org/10.1042/bio02505019.

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The tissue engineering that is now emerging in biomedical research groups is concerned with living tissues and how we can harness biological processes to achieve healing and repair, where it is otherwise failing. It aims to develop our scientific understanding of how living cells function, so that we can gain control and direct their activity to the promote the repair of damaged and diseased tissue1.

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8

Sahoo, Sambit, Thomas KH Teh, Pengfei He, Siew Lok Toh e James CH Goh. "Interface Tissue Engineering: Next Phase in Musculoskeletal Tissue Repair". Annals of the Academy of Medicine, Singapore 40, n.º 5 (15 de maio de 2011): 245–51. http://dx.doi.org/10.47102/annals-acadmedsg.v40n5p245.

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Increasing incidence of musculoskeletal injuries coupled with limitations in the current treatment options have necessitated tissue engineering and regenerative medicine- based approaches. Moving forward from engineering isolated musculoskeletal tissues, research strategies are now being increasingly focused on repairing and regenerating the interfaces between dissimilar musculoskeletal tissues with the aim to achieve seamless integration of engineered musculoskeletal tissues. This article reviews the state-of-the-art in the tissue engineering of musculoskeletal tissue interfaces with a focus on Singapore’s contribution in this emerging field. Various biomimetic scaffold and cell-based strategies, the use of growth factors, gene therapy and mechanical loading, as well as animal models for functional validation of the tissue engineering strategies are discussed. Keywords: Functional tissue engineering, Orthopaedic interfaces, Regenerative medicine, Scaffolds

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9

Goud, K. Anand. "Necrotizing Soft Tissue Infections". Journal of Medical Science And clinical Research 05, n.º 02 (10 de fevereiro de 2017): 17509–13. http://dx.doi.org/10.18535/jmscr/v5i2.49.

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10

Francisco, George, Joel Alan e Benjamin Dylan. "The Partial Tissue Expansions". Dermatology and Dermatitis 2, n.º 3 (15 de abril de 2018): 01–02. http://dx.doi.org/10.31579/2578-8949/030.

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Background: Tissue expanders are usually inflated with saline. We attempted to mitigate the side effects of the weight of the tissue expanders by replacing some of the saline with air. Methods: Of the 23 patients who were implanted with tissue expanders at our hospital, 7 complained of discomfort resulting from awareness of implant expansion and consciousness of implant weight, and 3 showed marked malposition. For these 10 patients, we replaced some of the saline with air to alleviate their symptoms. Results: Symptoms improved in all 10 patients without complications, and their tissue expanders were eventually replaced with permanent implants. Conclusions: No difference was observed between the 10 patients with tissue expanders inflated partially with air and the 13 for whom, only saline was used. Inflating tissue expanders with a mixture of air and saline is a good way to prevent side effects related to expander weight.

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11

Tezcaner, A., G. Köse e V. Hasırcı. "Fundamentals of tissue engineering: Tissues and applications". Technology and Health Care 10, n.º 3-4 (8 de julho de 2002): 203–16. http://dx.doi.org/10.3233/thc-2002-103-406.

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Patil, Amol Somaji, Yash Merchant e Preethi Nagarajan. "Tissue Engineering of Craniofacial Tissues – A Review". journal of Regenerative Medicine and Tissue Engineering 2, n.º 1 (2013): 6. http://dx.doi.org/10.7243/2050-1218-2-6.

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13

Duance, Vic. "Connective tissue: Get connected with connective tissues". Biochemist 25, n.º 5 (1 de outubro de 2003): 7–10. http://dx.doi.org/10.1042/bio02505007.

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14

Leong, Ivone. "New tissue processing technique for adipose tissues". Nature Reviews Endocrinology 14, n.º 3 (29 de janeiro de 2018): 128. http://dx.doi.org/10.1038/nrendo.2018.8.

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15

Yoshizato, Katsutoshi. "Tissue reconstitution: metamorphosis, regeneration, and artificial tissues". Wound Repair and Regeneration 6, n.º 4 (julho de 1998): 273–75. http://dx.doi.org/10.1046/j.1524-475x.1998.60403.x.

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16

Villar, Cristina C., e David L. Cochran. "Regeneration of Periodontal Tissues: Guided Tissue Regeneration". Dental Clinics of North America 54, n.º 1 (janeiro de 2010): 73–92. http://dx.doi.org/10.1016/j.cden.2009.08.011.

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17

Rickles, Richard J., e Sidney Strickland. "Tissue plasminogen activator mRNA in murine tissues". FEBS Letters 229, n.º 1 (29 de fevereiro de 1988): 100–106. http://dx.doi.org/10.1016/0014-5793(88)80806-8.

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18

Atala, Anthony. "Tissue engineering of reproductive tissues and organs". Fertility and Sterility 98, n.º 1 (julho de 2012): 21–29. http://dx.doi.org/10.1016/j.fertnstert.2012.05.038.

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19

McCullen, Seth D., Andre GY Chow e Molly M. Stevens. "In vivo tissue engineering of musculoskeletal tissues". Current Opinion in Biotechnology 22, n.º 5 (outubro de 2011): 715–20. http://dx.doi.org/10.1016/j.copbio.2011.05.001.

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20

Boschetti, Federica. "Tissue Mechanics and Tissue Engineering". Applied Sciences 12, n.º 13 (30 de junho de 2022): 6664. http://dx.doi.org/10.3390/app12136664.

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Tissue engineering (TE) combines scaffolds, cells, and chemical and physical cues to replace biological tissues. Several disciplines, such as biology, chemistry, materials science, mathematics, and most branches of engineering, support this goal while improving the quality of the reconstructed tissues [...]

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21

Feng, Wei, Yoke San Wong e Dietmar W. Hutmacher. "The Application of Image Processing Software for Tissue Engineering(Cellular & Tissue Engineering)". Proceedings of the Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics 2004.1 (2004): 95–96. http://dx.doi.org/10.1299/jsmeapbio.2004.1.95.

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22

Rhee, Sung-Mi, Hi-Jin You e Seung-Kyu Han. "Injectable Tissue-Engineered Soft Tissue for Tissue Augmentation". Journal of Korean Medical Science 29, Suppl 3 (2014): S170. http://dx.doi.org/10.3346/jkms.2014.29.s3.s170.

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23

Bove, Mary, Annalisa Carlucci, Giovanni Natale, Chiara Freda, Antonio Noro, Vincenzo Ferrara, Giorgia Opromolla et al. "Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature". Surgeries 2, n.º 1 (28 de janeiro de 2021): 58–82. http://dx.doi.org/10.3390/surgeries2010005.

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Tissue engineering refers to the attempt to create functional human tissue from cells in a laboratory. This is a field that uses living cells, biocompatible materials, suitable biochemical and physical factors, and their combinations to create tissue-like structures. To date, no tissue engineered skeletal muscle implants have been developed for clinical use, but they may represent a valid alternative for the treatment of volumetric muscle loss in the near future. Herein, we reviewed the literature and showed different techniques to produce synthetic tissues with the same architectural, structural and functional properties as native tissues.

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24

Alsberg, E., E. E. Hill e D. J. Mooney. "Craniofacial Tissue Engineering". Critical Reviews in Oral Biology & Medicine 12, n.º 1 (janeiro de 2001): 64–75. http://dx.doi.org/10.1177/10454411010120010501.

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There is substantial need for the replacement of tissues in the craniofacial complex due to congenital defects, disease, and injury. The field of tissue engineering, through the application of engineering and biological principles, has the potential to create functional replacements for damaged or pathologic tissues. Three main approaches to tissue engineering have been pursued: conduction, induction by bioactive factors, and cell transplantation. These approaches will be reviewed as they have been applied to key tissues in the craniofacial region. While many obstacles must still be overcome prior to the successful clinical restoration of tissues such as skeletal muscle and the salivary glands, significant progress has been achieved in the development of several tissue equivalents, including skin, bone, and cartilage. The combined technologies of gene therapy and drug delivery with cell transplantation will continue to increase treatment options for craniofacial cosmetic and functional restoration.

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25

Rosati, Adolfo, Silvia Caporali, Sofiene B. M. Hammami, Inmaculada Moreno-Alías, Andrea Paoletti e Hava F. Rapoport. "Tissue size and cell number in the olive (Olea europaea) ovary determine tissue growth and partitioning in the fruit". Functional Plant Biology 39, n.º 7 (2012): 580. http://dx.doi.org/10.1071/fp12114.

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The relationship between tissue size and cell number in the ovary and tissue size in the fruit, was studied in eight olive (Olea europaea L.) cultivars with different fruit and ovary size. All tissues in the ovary increased in size with increasing ovary size. Tissue size in the fruits correlated with tissue size in the ovary for both mesocarp and endocarp, but with different correlations: the mesocarp grew about twice as much per unit of initial volume in the ovary. Tissue size in the fruit also correlated with tissue cell number in the ovary. In this case, a single regression fitted all data pooled for both endocarp and mesocarp, implying that a similar tissue mass was obtained in the fruit per initial cell in the ovary, independent of tissues and cultivars. Tissue relative growth from bloom to harvest (i.e. the ratio between final and initial tissue size) differed among cultivars and tissues, but correlated with tissue cell size at bloom, across cultivars and tissues. These results suggest that in olive, tissue growth and partitioning in the fruit is largely determined by the characteristics of the ovary tissues at bloom, providing important information for plant breeding and crop management.

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26

Butler, David L., Natalia Juncosa-Melvin, John West, Jason Shearn, Marc Galloway, Greg Boivin, Victor Nirmalanandhan e Gindi Gooch. "Functional Tissue Engineering for Soft Tissue Repair : Matching In Vivo Biomechanics(International Workshop 3)". Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2005.18 (2006): 4–5. http://dx.doi.org/10.1299/jsmebio.2005.18.4.

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27

Toh, S. L., S. W. Goh, S. Y. Lau, W. L. Teng, J. C. Goh, H. W. Ouyang e T. E. Tay. "Mechanical Characterisation of Knitted/Woven Scaffolds for Tissue Engineering Applications(Cellular & Tissue Engineering)". Proceedings of the Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics 2004.1 (2004): 97–98. http://dx.doi.org/10.1299/jsmeapbio.2004.1.97.

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28

Narayan, R. P. "Development of tissue bank". Indian Journal of Plastic Surgery 45, n.º 02 (maio de 2012): 396–402. http://dx.doi.org/10.4103/0970-0358.101326.

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ABSTRACTThe history of tissue banking is as old as the use of skin grafting for resurfacing of burn wounds. Beneficial effects of tissue grafts led to wide spread use of auto and allograft for management of varied clinical conditions like skin wounds, bone defects following trauma or tumor ablation. Availability of adequate amount of tissues at the time of requirement was the biggest challenge that forced clinicians to find out techniques to preserve the living tissue for prolonged period of time for later use and thus the foundation of tissue banking was started in early twentieth century. Harvesting, processing, storage and transportation of human tissues for clinical use is the major activity of tissue banks. Low temperature storage of processed tissue is the best preservation technique at present. Tissue banking organization is a very complex system and needs high technical expertise and skilled personnel for proper functioning in a dedicated facility. A small lapse/deviation from the established protocol leads to loss of precious tissues and or harm to recipients as well as the risk of transmission of deadly diseases and tumors. Strict tissue transplant acts and stringent regulations help to streamline the whole process of tissue banking safe for recipients and to community as whole.

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Hauser, Peter Viktor, Hsiao-Min Chang, Masaki Nishikawa, Hiroshi Kimura, Norimoto Yanagawa e Morgan Hamon. "Bioprinting Scaffolds for Vascular Tissues and Tissue Vascularization". Bioengineering 8, n.º 11 (6 de novembro de 2021): 178. http://dx.doi.org/10.3390/bioengineering8110178.

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In recent years, tissue engineering has achieved significant advancements towards the repair of damaged tissues. Until this day, the vascularization of engineered tissues remains a challenge to the development of large-scale artificial tissue. Recent breakthroughs in biomaterials and three-dimensional (3D) printing have made it possible to manipulate two or more biomaterials with complementary mechanical and/or biological properties to create hybrid scaffolds that imitate natural tissues. Hydrogels have become essential biomaterials due to their tissue-like physical properties and their ability to include living cells and/or biological molecules. Furthermore, 3D printing, such as dispensing-based bioprinting, has progressed to the point where it can now be utilized to construct hybrid scaffolds with intricate structures. Current bioprinting approaches are still challenged by the need for the necessary biomimetic nano-resolution in combination with bioactive spatiotemporal signals. Moreover, the intricacies of multi-material bioprinting and hydrogel synthesis also pose a challenge to the construction of hybrid scaffolds. This manuscript presents a brief review of scaffold bioprinting to create vascularized tissues, covering the key features of vascular systems, scaffold-based bioprinting methods, and the materials and cell sources used. We will also present examples and discuss current limitations and potential future directions of the technology.

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Kouadjo, Kouame E., Yuichiro Nishida, Jean F. Cadrin-Girard, Mayumi Yoshioka e Jonny St-Amand. "Housekeeping and tissue-specific genes in mouse tissues". BMC Genomics 8, n.º 1 (2007): 127. http://dx.doi.org/10.1186/1471-2164-8-127.

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Caplan, Arnold I., e Victor M. Goldberg. "Principles of Tissue Engineered Regeneration of Skeletal Tissues". Clinical Orthopaedics and Related Research 367 (outubro de 1999): S12—S16. http://dx.doi.org/10.1097/00003086-199910001-00003.

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32

Martin, I., R. Quarto, B. Dozin e R. Cancedda. "Producing prefabricated tissues and organs via tissue engineering". IEEE Engineering in Medicine and Biology Magazine 16, n.º 2 (1997): 73–80. http://dx.doi.org/10.1109/51.582179.

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Niederberger, Craig. "Re: Tissue Engineering of Reproductive Tissues and Organs". Journal of Urology 189, n.º 3 (março de 2013): 1038. http://dx.doi.org/10.1016/j.juro.2012.11.137.

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Mardon, Helen J., e James T. Triffitt. "A tissue-specific protein in rat osteogenic tissues". Journal of Bone and Mineral Research 2, n.º 3 (3 de dezembro de 2009): 191–99. http://dx.doi.org/10.1002/jbmr.5650020305.

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Noda, Sawako, Yoshinori Sumita, Seigo Ohba, Hideyuki Yamamoto e Izumi Asahina. "Soft tissue engineering with micronized-gingival connective tissues". Journal of Cellular Physiology 233, n.º 1 (3 de maio de 2017): 249–58. http://dx.doi.org/10.1002/jcp.25871.

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J, Mancini-Filho. "Natural Antioxidants and Tissue Inflammation". Bioequivalence & Bioavailability International Journal 7, n.º 2 (4 de julho de 2023): 1–3. http://dx.doi.org/10.23880/beba-16000203.

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The phenolic compounds present in food cover a wide range of structures that have different biological properties. Highlighting its antioxidant properties and the presence mainly of spices, herbs and other foods. Some compounds present in spices can be listed for their antioxidant activity, such as: cloves have eugenol, pinene in their composition, cinnamon also has eugenol, limonene, pinene, catechins and other phenolic compounds in their composition, anise has pinene, rutin, apigenin, oregano has apigenin, quercecin, rosmarinic, caffeic, p-coumaric acids, and others. Rosemary presents the carnosic, rosmarinic, caffeic and hydroxycinnamic. The tissue inflammatory process normally starts with the presence of free radicals that are associated with the oxidative process activated by reactive oxygen species represented by peroxides, superoxide ion, presence of hydroxyl radical, singlet oxygen, among others. The highlighted phenolic compounds have in their structure one or more hydroxyls that have the property of donating a hydrogen atom to free radical structures, which can block the triggering of the oxidative process and thus inflammation.

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Matoka, Derek J., e Earl Y. Cheng. "Tissue engineering in urology". Canadian Urological Association Journal 3, n.º 5 (1 de maio de 2013): 403. http://dx.doi.org/10.5489/cuaj.1155.

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Tissue engineering encompasses a multidisciplinary approach gearedtoward the development of biological substitutes designed to restoreand maintain normal function in diseased or injured tissues. Thisarticle reviews the basic technology that is used to generateimplantable tissue-engineered grafts in vitro that will exhibit characteristicsin vivo consistent with the physiology and function ofthe equivalent healthy tissue. We also examine the current trendsin tissue engineering designed to tailor scaffold construction, promoteangiogenesis and identify an optimal seeded cell source.Finally, we describe several currently applied therapeutic modalitiesthat use a tissue-engineered construct. While notable progresshas clearly been demonstrated in this emerging field, these effortshave not yet translated into widespread clinical applicability. Withcontinued development and innovation, there is optimism that thetremendous potential of this field will be realized.L’ingénierie tissulaire englobe une approche multidisciplinaireaxée sur le développement de substituts biologiques en vue derétablir et de maintenir la fonction normale de tissus lésés. L’articlequi suit passe en revue la technologie fondamentale utilisée pourgénérer des greffons implantables produits par ingénierie in vitroet possédant des caractéristiques in vivo correspondant aux tissussains équivalents sur les plans physiologique et fonctionnel.Nous examinons également les tendances actuelles en ingénierietissulaire visant à adapter des échafaudages tissulaires, à promouvoirl’angiogenèse et à dégager une source optimale de cellulesimplantables. Enfin, nous décrivons plusieurs modalités thérapeutiquesactuellement mises en application utilisant un échafaudagecréé par ingénierie tissulaire. En dépit de progrès remarquablesdans ce domaine en effervescence, les efforts déployés ne se sontpas encore traduits par une applicabilité clinique étendue. Desdéveloppements et des percées continus permettent d’être optimisteface au potentiel prodigieux de ce domaine.

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Iwata, Takanori, Masayuki Yamato, Isao Ishikawa, Tomohiro Ando e Teruo Okano. "Tissue Engineering in Periodontal Tissue". Anatomical Record 297, n.º 1 (2 de dezembro de 2013): 16–25. http://dx.doi.org/10.1002/ar.22812.

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Czeczot, Hanna, Dorota Scibior, Michał Skrzycki e Małgorzata Podsiad. "Glutathione and GSH-dependent enzymes in patients with liver cirrhosis and hepatocellular carcinoma." Acta Biochimica Polonica 53, n.º 1 (9 de janeiro de 2006): 237–41. http://dx.doi.org/10.18388/abp.2006_3384.

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We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.

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Yao, Lan, Wenhua Zhang, Xuedong Wang, Lishuang Guo, Wenlu Liu, Yueyue Li, Rui Ma et al. "Orbital Adipose Tissue: The Optimal Control for Back-Table Fluorescence Imaging of Orbital Tumors". Bioengineering 11, n.º 9 (14 de setembro de 2024): 922. http://dx.doi.org/10.3390/bioengineering11090922.

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Control tissue is essential for ensuring the precision of semiquantitative analysis in back-table fluorescence imaging. However, there remains a lack of agreement on the appropriate selection of control tissues. To evaluate the back-table fluorescence imaging performance of different normal tissues and identify the optimal normal tissue, a cohort of 39 patients with orbital tumors were enrolled in the study. Prior to surgery, these patients received indocyanine green (ICG) and following resection, 43 normal control tissues (34 adipose tissues, 3 skin tissues, 3 periosteal tissues, and 3 muscle tissues) were examined using back-table fluorescence imaging. The skin tissue demonstrated significantly elevated fluorescence intensity in comparison to the diseased tissue, whereas the muscle tissue exhibited a broad range and standard deviation of fluorescence signal intensity. Conversely, the adipose and periosteum displayed weak fluorescence signals with a relatively consistent distribution. Additionally, no significant correlations were found between the signal-to-background ratio (SBR) of adipose tissue and patients’ ages, genders, weights, disease duration, tumor origins, dosing of administration of ICG infusion, and the time interval between ICG infusion and surgery. However, a positive correlation was observed between the SBR of adipose tissue and its size, with larger adipose tissues (>1 cm) showing an average SBR 27% higher than smaller adipose tissues (≤1 cm). In conclusion, the findings of this study demonstrated that adipose tissue consistently exhibited homogeneous hypofluorescence during back-table fluorescence imaging, regardless of patient clinical variables or imaging parameters. The size of the adipose tissue was identified as the primary factor influencing its fluorescence imaging characteristics, supporting its utility as an ideal control tissue for back-table fluorescence imaging.

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Ikada, Yoshito. "Challenges in tissue engineering". Journal of The Royal Society Interface 3, n.º 10 (18 de abril de 2006): 589–601. http://dx.doi.org/10.1098/rsif.2006.0124.

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Almost 30 years have passed since a term ‘tissue engineering’ was created to represent a new concept that focuses on regeneration of neotissues from cells with the support of biomaterials and growth factors. This interdisciplinary engineering has attracted much attention as a new therapeutic means that may overcome the drawbacks involved in the current artificial organs and organ transplantation that have been also aiming at replacing lost or severely damaged tissues or organs. However, the tissues regenerated by this tissue engineering and widely applied to patients are still very limited, including skin, bone, cartilage, capillary and periodontal tissues. What are the reasons for such slow advances in clinical applications of tissue engineering? This article gives the brief overview on the current tissue engineering, covering the fundamentals and applications. The fundamentals of tissue engineering involve the cell sources, scaffolds for cell expansion and differentiation and carriers for growth factors. Animal and human trials are the major part of the applications. Based on these results, some critical problems to be resolved for the advances of tissue engineering are addressed from the engineering point of view, emphasizing the close collaboration between medical doctors and biomaterials scientists.

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Bhave, Gautam. "Quantitating excess tissue sodium". Clinical Science 133, n.º 6 (março de 2019): 739–40. http://dx.doi.org/10.1042/cs20190037.

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Abstract Using changes in tissue [Na+] concentration alone as done with Na+ MRI may not accurately quantitate excess tissue Na+, particularly in cellular tissues. However, individually quantitating alterations in tissue Na+ and water content as possible with ashing studies may still accurately quantitate excess tissue Na+ in these situations. Furthermore, when tissue [Na+] exceeds plasma [Na+], excess tissue Na+ must be present.

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Lahiri, Pooja, Suranjana Mukherjee, Biswajoy Ghosh, Debnath Das, Basudev Lahiri, Shailendra Kumar Varshney, Mousumi Pal, Ranjan Rashmi Paul e Jyotirmoy Chatterjee. "Comprehensive Evaluation of PAXgene Fixation on Oral Cancer Tissues Using Routine Histology, Immunohistochemistry, and FTIR Microspectroscopy". Biomolecules 11, n.º 6 (15 de junho de 2021): 889. http://dx.doi.org/10.3390/biom11060889.

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The choice of tissue fixation is critical for preserving the morphology and biochemical information of tissues. Fragile oral tissues with lower tensile strength are challenging to process for histological applications as they are prone to processing damage, such as tissue tear, wrinkling, and tissue fall-off from slides. This leads to loss of morphological information and unnecessary delay in experimentation. In this study, we have characterized the new PAXgene tissue fixation system on oral buccal mucosal tissue of cancerous and normal pathology for routine histological and immunohistochemical applications. We aimed to minimize the processing damage of tissues and improve the quality of histological experiments. We also examined the preservation of biomolecules by PAXgene fixation using FTIR microspectroscopy. Our results demonstrate that the PAXgene-fixed tissues showed significantly less tissue fall-off from slides. Hematoxylin and Eosin staining showed comparable morphology between formalin-fixed and PAXgene-fixed tissues. Good quality and slightly superior immunostaining for cancer-associated proteins p53 and CK5/6 were observed in PAXgene-fixed tissues without antigen retrieval than formalin-fixed tissues. Further, FTIR measurements revealed superior preservation of glycogen, fatty acids, and amide III protein secondary structures in PAXgene-fixed tissues. Overall, we present the first comprehensive evaluation of the PAXgene tissue fixation system in oral tissues. This study concludes that the PAXgene tissue fixation system can be applied to oral tissues to perform diagnostic molecular pathology experiments without compromising the quality of the morphology or biochemistry of biomolecules.

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Solovyov, V. G., Yu M. Lankin e I. Yu Romanova. "Skin-effect in soft biological tissue and features of tissue heating during automatic bipolar welding". Paton Welding Journal 2021, n.º 7 (28 de julho de 2021): 24–29. http://dx.doi.org/10.37434/tpwj2021.07.05.

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Klimczak, Aleksandra, e Urszula Kozlowska. "Mesenchymal Stromal Cells and Tissue-Specific Progenitor Cells: Their Role in Tissue Homeostasis". Stem Cells International 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/4285215.

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Multipotent mesenchymal stromal/stem cells (MSCs) reside in many human organs and comprise heterogeneous population of cells with self-renewal ability. These cells can be isolated from different tissues, and their morphology, immunophenotype, and differentiation potential are dependent on their tissue of origin. Each organ contains specific population of stromal cells which maintain regeneration process of the tissue where they reside, but some of them have much more wide plasticity and differentiate into multiple cells lineage. MSCs isolated from adult human tissues are ideal candidates for tissue regeneration and tissue engineering. However, MSCs do not only contribute to structurally tissue repair but also MSC possess strong immunomodulatory and anti-inflammatory properties and may influence in tissue repair by modulation of local environment. This paper is presenting an overview of the current knowledge of biology of tissue-resident mesenchymal stromal and progenitor cells (originated from bone marrow, liver, skeletal muscle, skin, heart, and lung) associated with tissue regeneration and tissue homeostasis.

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Lugli, Alessandro, Yvonne Forster, Philippe Haas, Antoinio Nocito, Christoph Bucher, Heidi Bissig, Martina Mirlacher, Martina Storz, Michael J. Mihatsch e Guido Sauter. "Calretinin expression in human normal and neoplastic tissues: a tissue microarray analysis on 5233 tissue samples". Human Pathology 34, n.º 10 (outubro de 2003): 994–1000. http://dx.doi.org/10.1053/s0046-8177(03)00339-3.

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Abedi, Niloufar, Zahra Sadat Sajadi-Javan, Monireh Kouhi, Legha Ansari, Abbasali Khademi e Seeram Ramakrishna. "Antioxidant Materials in Oral and Maxillofacial Tissue Regeneration: A Narrative Review of the Literature". Antioxidants 12, n.º 3 (27 de fevereiro de 2023): 594. http://dx.doi.org/10.3390/antiox12030594.

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Oral and maxillofacial tissue defects caused by trauma, tumor reactions, congenital anomalies, ischemic diseases, infectious diseases, surgical resection, and odontogenic cysts present a formidable challenge for reconstruction. Tissue regeneration using functional biomaterials and cell therapy strategies has raised great concerns in the treatment of damaged tissue during the past few decades. However, during biomaterials implantation and cell transplantation, the production of excessive reactive oxygen species (ROS) may hinder tissue repair as it commonly causes severe tissue injuries leading to the cell damage. These products exist in form of oxidant molecules such as hydrogen peroxide, superoxide ions, hydroxyl radicals, and nitrogen oxide. These days, many scientists have focused on the application of ROS-scavenging components in the body during the tissue regeneration process. One of these scavenging components is antioxidants, which are beneficial materials for the treatment of damaged tissues and keeping tissues safe against free radicals. Antioxidants are divided into natural and synthetic sources. In the current review article, different antioxidant sources and their mechanism of action are discussed. The applications of antioxidants in the regeneration of oral and maxillofacial tissues, including hard tissues of cranial, alveolar bone, dental tissue, oral soft tissue (dental pulp, periodontal soft tissue), facial nerve, and cartilage tissues, are also highlighted in the following parts.

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Owida, Hamza Abu, Jamal I. Al-Nabulsi, Feras Alnaimat, Muhammad Al-Ayyad, Nidal M. Turab, Ashraf Al Sharah e Murad Shakur. "Recent Applications of Electrospun Nanofibrous Scaffold in Tissue Engineering". Applied Bionics and Biomechanics 2022 (9 de fevereiro de 2022): 1–15. http://dx.doi.org/10.1155/2022/1953861.

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Tissue engineering is a relatively new area of research that combines medical, biological, and engineering fundamentals to create tissue-engineered constructs that regenerate, preserve, or slightly increase the functions of tissues. To create mature tissue, the extracellular matrix should be imitated by engineered structures, allow for oxygen and nutrient transmission, and release toxins during tissue repair. Numerous recent studies have been devoted to developing three-dimensional nanostructures for tissue engineering. One of the most effective of these methods is electrospinning. Numerous nanofibrous scaffolds have been constructed over the last few decades for tissue repair and restoration. The current review gives an overview of attempts to construct nanofibrous meshes as tissue-engineered scaffolds for various tissues such as bone, cartilage, cardiovascular, and skin tissues. Also, the current article addresses the recent improvements and difficulties in tissue regeneration using electrospinning.

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Pei, Guangsheng, Yulin Dai, Zhongming Zhao e Peilin Jia. "deTS: tissue-specific enrichment analysis to decode tissue specificity". Bioinformatics 35, n.º 19 (1 de março de 2019): 3842–45. http://dx.doi.org/10.1093/bioinformatics/btz138.

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Abstract Motivation Diseases and traits are under dynamic tissue-specific regulation. However, heterogeneous tissues are often collected in biomedical studies, which reduce the power in the identification of disease-associated variants and gene expression profiles. Results We present deTS, an R package, to conduct tissue-specific enrichment analysis with two built-in reference panels. Statistical methods are developed and implemented for detecting tissue-specific genes and for enrichment test of different forms of query data. Our applications using multi-trait genome-wide association studies data and cancer expression data showed that deTS could effectively identify the most relevant tissues for each query trait or sample, providing insights for future studies. Availability and implementation https://github.com/bsml320/deTS and CRAN https://cran.r-project.org/web/packages/deTS/ Supplementary information Supplementary data are available at Bioinformatics online.

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Inyushin, Mikhail, Daria Meshalkina, Lidia Zueva e Astrid Zayas-Santiago. "Tissue Transparency In Vivo". Molecules 24, n.º 13 (28 de junho de 2019): 2388. http://dx.doi.org/10.3390/molecules24132388.

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In vivo tissue transparency in the visible light spectrum is beneficial for many research applications that use optical methods, whether it involves in vivo optical imaging of cells or their activity, or optical intervention to affect cells or their activity deep inside tissues, such as brain tissue. The classical view is that a tissue is transparent if it neither absorbs nor scatters light, and thus absorption and scattering are the key elements to be controlled to reach the necessary transparency. This review focuses on the latest genetic and chemical approaches for the decoloration of tissue pigments to reduce visible light absorption and the methods to reduce scattering in live tissues. We also discuss the possible molecules involved in transparency.

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