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Littérature scientifique sur le sujet « Enzyme-linked immunoassays »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 26 juillet 2025

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Articles de revues sur le sujet "Enzyme-linked immunoassays"

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Bai J K, Sonia, N. Midhun, Chakrapani K V, Haripriya G, Chandra Kiran Venkata Sai Pathuri, and N. Surya Ramalakshmi. "Enzyme linked immunosorbantassay- lab diagnosis: A review." Indian Journal of Microbiology Research 8, no. 1 (2021): 10–14. http://dx.doi.org/10.18231/j.ijmr.2021.003.

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Immunoassays are generally antigen antibody analytical methods used for quantitative or for qualitative analysis. They are most commonly used for diagnostic purposes, drug monitoring in pharmacokinetic studies and in the quality control. ELISA is the most common used immunoassay where an enzyme is linked to detect the antibodies in the blood. ELISA tests are more accurate compared to other antibody-assays as it yields quantitative results. This review article describes ELISA and its applications, types and limitations.

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Azimi, Nooshin T., Fujiang Wen, Richard M. Lister, Dennis A. Chen, and Fred E. Lytle. "Enzyme-Linked Immunoassays Using Nanosecond Fluorometric Detection." Applied Spectroscopy 46, no. 6 (1992): 994–98. http://dx.doi.org/10.1366/0003702924124402.

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Nanosecond temporal resolution is combined with an enzyme-linked immunosorbent assay (ELISA) to improve the lower limit of detection for a plant virus, brome mosaic virus. The method uses alkaline phosphatase as the enzyme link and β-naphthyl phosphate as the substrate. Enzymatic activity produces the highly fluorescent tag β-naphthol. The 8.9-ns lifetime of the tag allows temporal discrimination against the assay blank, providing a 64× improvement in the detection limit as compared to a steady-state measurement, and a ∼4100× improvement over a standard ELISA method incorporating the chromogen

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Carr, Ronald I., Mervat Mansour, Damaso Sadi, Hana James, and John Verrier Jones. "A substrate amplification system for enzyme-linked immunoassays." Journal of Immunological Methods 98, no. 2 (1987): 201–8. http://dx.doi.org/10.1016/0022-1759(87)90006-8.

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Carr, Ronald I., Mervat Mansour, Damaso Sadi, Hana James, and John Verrier Jones. "A substrate amplification system for enzyme-linked immunoassays." Journal of Immunological Methods 103, no. 1 (1987): 157. http://dx.doi.org/10.1016/0022-1759(87)90259-6.

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Verrier Jones, J., M. Mansour, H. James, D. Sadi, and R. I. Carr. "A substrate amplification system for enzyme-linked immunoassays." Journal of Immunological Methods 118, no. 1 (1989): 79–84. http://dx.doi.org/10.1016/0022-1759(89)90056-2.

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Ehm, M., and A. Kappel. "Immunoassays for diagnosis of coagulation disorders." Hämostaseologie 30, no. 04 (2010): 194–201. http://dx.doi.org/10.1055/s-0037-1619055.

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SummaryImmunoassays play a pivotal role in the clinical laboratory. In the coagulation section of the laboratory, they are used as an aid for diagnosis of deep vein thrombosis or pulmonary embolism, thrombophilia screening, or detection of coagulation factor deficiencies, respectively. Enzyme-linked immunosorbent assay (ELISA) and latex agglutination immunoassay technologies are currently most widely used, while Luminescent Oxygen Channeling Immuno - assay (LOCI®) and other chemiluminescencebased immunoassays are emerging technologies for the coagulation laboratory. However, not all immunoassa

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Zhou, Lu, Yifan Liu, Yang Lu, et al. "Recent Advances in the Immunoassays Based on Nanozymes." Biosensors 12, no. 12 (2022): 1119. http://dx.doi.org/10.3390/bios12121119.

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As a rapid and simple method for the detection of multiple targets, immunoassay has attracted extensive attention due to the merits of high specificity and sensitivity. Notably, enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay, which can provide high detection sensitivity since the enzyme labels can promote the generation of catalytically amplified readouts. However, the natural enzyme labels usually suffer from low stability, high cost, and difficult storage. Inspired by the advantages of superior and tunable catalytic activities, easy preparation, low cost, and high sta

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Rajesh, K. Sharma, K. Sharma Pankaj, Talat Gaush, Gautam Praveen, Chhabra Reba, and Singh Surinder. "BRIEF OVERVIEW ON HEPATITIS C VIRUS IMMUNOASSAYS." International Journal of Research – Granthaalayah 4, no. 1 (2017): 178–84. https://doi.org/10.5281/zenodo.848525.

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The publication deals with a brief overview of Hepatitis C Virus (HCV) and donor blood screening for HCV by using conventional Rapid, Enzyme Linked Immunosorbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA) also. The advantages of various generation o

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Mutayoba, B. M., H. H. D. Meyer, D. Schams, and E. Schallenberger. "Development of a sensitive enzyme immunoassay for LH determination in bovine plasma using the streptavidin-biotin technique." Acta Endocrinologica 122, no. 2 (1990): 227–32. http://dx.doi.org/10.1530/acta.0.1220227.

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Abstract Using the biotin-streptavidin amplification technique, highly sensitive specific second-antibody enzyme immunoassays for determining LH in bovine plasma with long (48 h) and short (4 h) incubation periods were developed. Biotin was linked to bLH by the N-hydroxysuccimidine method and the product (biotinyl-bLH) used to bridge between streptavidin-peroxidase and the immobilised bLH antibody in competitive tests. The assays were validated and their performance compaired with a radioimmunoassay currently in use. The sensitivities of the long and short incubation enzyme immunoassays (8 pg

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Liu, Lin, Yuanqiang Hao, Dehua Deng, and Ning Xia. "Nanomaterials-Based Colorimetric Immunoassays." Nanomaterials 9, no. 3 (2019): 316. http://dx.doi.org/10.3390/nano9030316.

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Colorimetric immunoassays for tumor marker detection have attracted considerable attention due to their simplicity and high efficiency. With the achievements of nanotechnology and nanoscience, nanomaterials-based colorimetric immunoassays have been demonstrated to be promising alternatives to conventional colorimetric enzyme-linked immunoassays. This review is focused on the progress in colorimetric immunoassays with the signal amplification of nanomaterials, including nanomaterials-based artificial enzymes to catalyze the chromogenic reactions, analyte-induced aggregation or size/morphology c

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Thèses sur le sujet "Enzyme-linked immunoassays"

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Carroll, Andrea D. "Development of bead injection methodology for immunoassays /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/8599.

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Kiote-Schmidt, Chrissoula. "Etablierung eines kompetitiven enzymgekoppelten Immunoassays zum Nachweis eines kleinen Peptids in Serum- und Liquorproben." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-59192.

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Booth, Ronald A. Carleton University Dissertation Biology. "Development of cloth-enzyme immunoassays for the detection of E. coli O157 and verotoxin." Ottawa, 1996.

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Schob, Stefan, Donald Lobsien, Benjamin Friedrich, et al. "The cerebral surfactant system and its alteration in hydrocephalic conditions." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-213883.

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Introduction: Pulmonary Surfactant reduces surface tension in the terminal airways thus facilitating breathing and contributes to host's innate immunity. Surfactant Proteins (SP) A, B, C and D were recently identified as inherent proteins of the CNS. Aim of the study was to investigate cerebrospinal fluid (CSF) SP levels in hydrocephalus patients compared to normal subjects. Patients and methods: CSF SP A-D levels were quantified using commercially available ELISA kits in 126 patients (0±84 years, mean 39 years). 60 patients without CNS pathologies served as a control group. Hydrocephalus pati

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Hardy, Charles Thomas. "Evaluation and calibration of enzyme immunoassays for detecting antibody to the human immunodeficiency virus and other agents /." Thesis, Connect to this title online; UW restricted, 1993. http://hdl.handle.net/1773/9297.

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von, Buttlar Heiner, Sabine Siegemund, Matthias Büttner, and Gottfried Alber. "Identification of toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells." Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-151442.

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Parapoxvirus ovis (PPVO) is known for its immunostimulatory capacities and has been successfully used to generate vector vaccines effective especially in non-permissive host species. Murine conventional and plasmacytoid dendritic cells (cDC and pDC) are able to recognize PPVO. The PPVO-sensing receptor on pDC is hitherto unknown. In this study we aimed to define the pattern recognition receptor responsible for the activation of murine pDC by inactivated and replication-competent PPVO. We show that PPVO-induced expression of type I and type III interferons, pro-inflammatory cytokines, and costi

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Orchard, Robert Graham. "A flow-through enzyme-linked immunoassay for progesterone." The University of Waikato, 2007. http://hdl.handle.net/10289/2277.

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Bovine reproductive performance is one of the most important factors influencing dairy farm profitability. Present-day techniques for oestrus- and pregnancy-detection are unreliable and labour-intensive. Although measuring milk-progesterone at regular intervals allows the fertility status of a cow to be determined reliably, the labour cost of collecting and analysing samples is prohibitive. This project aimed to develop a progesterone sensing system that could be automated and integrated with the milking unit, thus minimising labour costs. The proposed system involved mixing the milk sample wi

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Campbell, Charles Nelson. "Separationless immunoassay and DNA sensing using wired enzyme based amperometric affinity electrodes /." Digital version:, 2000. http://wwwlib.umi.com/cr/utexas/fullcit?p9992761.

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de, Alwis Wathuthanthirige Uditha. "Analytical applications of chemically modified antibodies." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184601.

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The components involved in an immunoassay were investigated in order to improve the detection limits of the ELISA and to make the assay adaptable to a flow injection analysis (FIA) configuration. The goal being the total automation of the ELISA procedure which is long, tedious and has high standard deviation. The antibody purification and cleavage methods were studied with special emphasis on obtaining products with highest immunological activity. The antibody-enzyme coupling reactions using homobifunctional reagents and heterobifunctional reagents were studied in order to attempt the preparat

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Lövgren, Ulf. "Enzyme immunoassay in combination with liquid chromatography for sensitive and selective determination of drugs in biosamples." Lund : Dept. of Analytical Chemistry, Lund University, 1997. http://books.google.com/books?id=Ju1qAAAAMAAJ.

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Livres sur le sujet "Enzyme-linked immunoassays"

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M, Kemeny D., and Challacombe Stephen J, eds. ELISA and other solid phase immunoassays: Theoretical and practical aspects. 1988., 1988.

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International Symposium on "Advances in Immunoassays for Veterinary and Food Analysis" (2nd 1986 University of Surrey). Immunoassays for veterinary and food analysis. Elsevier Applied Science, 1988.

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Marius, Teodorescu, and Froelich Christopher J, eds. Advanced immunoassays in rheumatology. CRC Press, 1994.

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Rothwell, E. An assessment of the value of IgM antibody to Hepatitis C virus using synthetic peptide enzyme immunoassays. University College Dublin, 1997.

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Eric, Koglin, Superfund Innovative Technology Evaluation Program (U.S.), Oak Ridge National Laboratory, and United States. Environmental Protection Agency, eds. Immunoassay kit: EnviroLogix, Inc. PCB in Soil Tube Assay. U.S. Environmental Protection Agency, Office of Research and Development, 1998.

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Koston, Suzanne C. Studies on the development of an Immunoassay to detect Neutrophil Collagenase (MMP-8). University College Dublin, 1997.

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E, Debus, ed. Control of immune response by endocrine factors: Malaria vaccine, controlled drug delivery, enzyme-immunoassay. Springer-Verlag, 1987.

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Pape, Carsten. Melatoningehalt in marinen Makroalgen: Entwicklung und Validierung quantitativer Bestimmungen mittels HPLC und Enzym-gekoppeltem Immunoassay = Melatonin in marine macroalgae : development and validation of quantitative determinations by HPLC and enzyme-linked immunosorbent assay. Alfred-Wegener-Institut für Polar- und Meeresforschung, 2004.

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S, Deshpande S. Enzyme Immunoassays: From Concept to Product Development. Springer London, Limited, 2012.

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S, Deshpande S. Enzyme Immunoassays: From Concept to Product Development. Springer, 2011.

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Chapitres de livres sur le sujet "Enzyme-linked immunoassays"

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Schmitt, K., Erwin Märtlbauer, Ewald Usleber, R. Gessler, J. Lepschy, and David Abramson. "Detection of Acetylated Deoxynivalenol by Enzyme-Linked Immunosorbent Assay." In Immunoassays for Residue Analysis. American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0621.ch023.

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Morsy, Mohamed A., Azza A. Ibrahim, and Mahmoud M. Hewedi. "Detection of Dieldrin by Enzyme-Linked Immunosorbent Assay in Some Dairy Products." In Immunoassays for Residue Analysis. American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0621.ch013.

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Bushway, Rodney J., Titan S. Fan, Lynn E. Katz, et al. "Development of an Enzyme-Linked Immunosorbent Assay for Hexazinone and Its Application to Water." In Immunoassays for Residue Analysis. American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0621.ch014.

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Morsy, Mohamed A., Azza A. Ibrahim, and Mahmoud M. Hewedi. "Detection of Pesticides in Human Milk Samples Collected in Egypt by Enzyme-Linked Immunosorbent Assay." In Immunoassays for Residue Analysis. American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0621.ch012.

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Maragos, Chris M., and Steven D. Miklasz. "Monoclonal Antibody-Based Competitive Enzyme-Linked Immunosorbent Assays for the Hydrolysis Product of Fumonisin B1(HFB1)." In Immunoassays for Residue Analysis. American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0621.ch027.

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Lopez-Avila, Viorica, Chatan Charan, and Jeanette Van Emon. "Supercritical Fluid Extraction—Enzyme-Linked Immunosorbent Assay Applications for Determination of Pesticides in Soil and Food." In Immunoassays for Residue Analysis. American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0621.ch035.

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Hsu, Hei-ti. "Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection." In Plant Pathology. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-062-1_2.

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Tsang, Victor C. W., George E. Bers, and Kathy Hancock. "Enzyme-Linked Immunoelectrotransfer Blot (EITB)." In Enzyme-Mediated Immunoassay. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5012-5_22.

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Butler, J. E., J. H. Peterman, and T. E. Koertge. "The Amplified Enzyme-Linked Immunosorbent Assay (a-ELISA)." In Enzyme-Mediated Immunoassay. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5012-5_14.

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Cimolai, Nevio. "Enzyme-Linked Immunoassay (Conventional Solid Phase)." In Serodiagnosis of the Infectious Diseases. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-5249-9_11.

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Actes de conférences sur le sujet "Enzyme-linked immunoassays"

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Brandhoff, L., M. J. Vellekoop, H. Zirath, et al. "C4.4 - Ultrasonic Dispersion of Particles in Lab-on-Chip Systems for Enzyme-Linked-Immunoassays." In AMA Conferences 2015. AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany, 2015. http://dx.doi.org/10.5162/sensor2015/c4.4.

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Lin, Samuel I. En. "A Study of Bifurcation Design Used in CD-ELISA Micro-Fluidic Platform." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13079.

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Enzyme-linked immunosorbent assays (ELISA), one of the most common immunoassays, is widely used for detection and quantification of chemical and biological molecules and is becoming more and more important in clinical diagnostics, food safety testing, and environmental monitoring. A major challenge in developing the CD-ELISA is to split the flow (e.g., bio-reagents) evenly on the micro-channels. The Coriolis force resultant from CD rotation can disturb the flow in the splitter region and thus cause the failure mode in delivering the solution from each reservoir in a pre-specified manner. In th

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Tanaka, I., A. Yoshioka, T. Fujiwara, H. Nakai, and H. Fukui. "THE CHANGES OF FACTOR VIII ANTIGEN DURING THE COAGULATION PROCESS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644038.

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The changes of factor VIII (F. VIII) during blood coagulation process is still controversial. We analyzed the F. VIII antigen (F. VIII:Ag) at various intervals of in vitro blood clotting by immunoassays using polyclonal and different kinds of monoclonal antibodies to F. VIII.We used two immunoassays, an immunoradiometric assay (IRMA) and an enzyme-linked immunosorbent assay (ELISA). The IRMA was performed by the method of Peake et al. using high-titer allo-antibodies to F. VIII. The ELISA was performed by two-site solid phase system consisting of alloantibodies as the first and one of three ki

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Váradi, K., J. Kárpáti, and S. Elödi. "ENZYME LINKED IMMUNOASSAY (ELISA) FOR FACTOR VIII ANTIGEN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644033.

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A two site ELISA test was developed for measuring factor VIII antigen (FVIIIsAg). The assay is based on two antibodies developed in a non-haemophilic and in a severe haemophilia-A patient, respectively. The IgG fraction prepared from the non-haemophilic plasma was used for coating, and the IgG isolated from the haemophilia-A plasma was labelled with horse-radish peroxidaseFVIII:Ag and FVIII activity was measured in 28 healthy blood donors and in 41 haemophilia-A patients. The normal range for FVIIIcAg was 40 - 180 %, the correlation coefficient between FVIII:Ag and FVIII activity assays was 0.

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Longinotti, G., G. Ybarra, P. Lloret, et al. "Diagnosis of foot-and-mouth disease by electrochemical enzyme-linked immunoassay." In 2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2010). IEEE, 2010. http://dx.doi.org/10.1109/iembs.2010.5626230.

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Sugihara, T., J. Takamatsu, T. Kamiya, H. Saito, K. Kimata, and K. Kato. "A SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR SERUM LAMININ." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643554.

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Laminin, a large glycoprotein, is a major and specific component of basement membrane. There were little or no circulating laminin in normal persons, although some recent reports have showed increased values in various diseases(ex. diabetes mellitus and liver disease) by a radioimmunoassay(RIA) using laminin fragment. The minimum detectable sensitivity of RIA was reported to be 20 ng/ml of serum sample. We describe here a more sensitive immunoassay system, and also the concentrations of laminin in sera from healthy subjects and patients. A sandwich ELISA method for measurement of laminin was e

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Lateef, Mehreen. "Determination of Serum C-reactive Protein Levels in Breast Cancer by Enzyme Linked Immunoassay Technique." In IBRAS 2021 INTERNATIONAL CONFERENCE ON BIOLOGICAL RESEARCH AND APPLIED SCIENCE. Juw, 2021. http://dx.doi.org/10.37962/ibras/2021/13.

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Triplett, D. A., J. T. Brant, K. Musgrave, and C. A. Orr. "RELATIONSHIP BETWEEN LUPUS ANTICOAGULANTS AND ANTIBODIES TO PHOSPHOLIPID." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643657.

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The relationship of lupus anticoagulants (LA's) to antibodies (IgG and IgM) to cardiolipin (CL), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidic acid (PA), detected by an enzyme linked immunoassay, was examined in a series of 100 patients with well characterized LA's. 73% of patients demonstrated antibodies to one or more phospholipids; 62% were positive for CL, 57% were positive for PS, 57% were positive for PI, and 51% were positive for PA. Of the samples with antibodies to phospholipid, 32% had IgG type antibody only, 16% had IgMonly, and 52% had both IgG and IgM antibod

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Shekha, Gawhar, Kalthum Maulood, and Mudhir Shekha. "Influence of Interleukin-6 174G>C gene Polymorphism on Patients with Stable Coronary Artery Disease." In 5th International Conference on Biomedical and Health Sciences. Cihan University-Erbil, 2024. http://dx.doi.org/10.24086/biohs2024/paper.1450.

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Background: Coronary artery disease is caused by plaque buildup in the arteries' walls that deliver blood to the heart. The study examined the correlation between the IL-6 gene promoter 174G>C polymorphism, blood IL-6 levels, and specific physiological parameters in Kurdish people from Iraq with stable coronary artery disease (CAD). Methods: The investigation took place on a sample of 62 persons diagnosed with stable coronary artery disease (SCAD) and a control group of 31 individuals without CAD. The Sanger sequencing method was employed to detect IL-6 SNP genes, and the enzyme-linked immu

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González Mota, Alba, Marina Covacho González, Isabel Valriberas Herrero, and Carlos Roncero Alonso. "Screening of cannabis use during pregnancy and neonates." In 22° Congreso de la Sociedad Española de Patología Dual (SEPD) 2020. SEPD, 2020. http://dx.doi.org/10.17579/sepd2020p090.

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Introduction: Cannabis use in pregnancy is related to developmental and mental disorders. The acknowledgement of prenatal exposure frequently depends on the mother’s report, which can often be omitted. There exists little description in the literature of the different methods to detect the use of cannabis during pregnancy. Moreover, nowadays there is no standardized screening available. Objectives: The objective is to analyze the different methods of prenatal screening of cannabis during pregnancy. Methods: A systematic review of studies on the methods of screening of cannabis use during pregn

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Rapports d'organisations sur le sujet "Enzyme-linked immunoassays"

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สิโรตมรัตน์, สัตถาพร, เครือวัลย์ พลจันทร та อัมพร ตันประเสริฐ. การตรวจหาดีเอ็นเอไวรัสตับอักเสบบีในซีรัมหญิงตั้งครรภ์ด้วยวิธี Polymerase chain reaction. จุฬาลงกรณ์มหาวิทยาลัย, 2001. https://doi.org/10.58837/chula.res.2001.29.

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นำตัวอย่างซีรัมหญิงตั้งครรภ์ทั้งหมด 512 ตัวอย่าง มาตรวจหาดีเอ็นเอส่วน S gene ของไวรัสตับอักเสบบี (HBV-DNA) ซึ่งเป็นแถบดีเอ็นเอขนาด 431 คู่เบส ด้วยวิธี Polymerase Chain Reaction (PCR) ศึกษาควบคู่กับการตรวจหาดัชนีในซีรัม (seromarkers) อื่นๆ ด้วยวิธีทางอิมมูโนแอสเสย์ ได้แก่ แอนติเจนชนิดผิวของไวรัสตับอักเสบบี (HBsAg) ภูมิต้านทานต่อแอนติเจนชนิดผิวของไวรัส (Anti-HBs) และ ภูมิต้านทานต่อส่วยแกน (core) ของอนุภาคไวรัส (Anti-HBC) การตรวจหา HBsAgใช้ 2 วิธี คือ Chemiluminescent immunoassay : Abbott prism HBsAg ประเทศเยอรมัน และ Enzyme-Linked Immunosorbent Assay (ELISA) ซึ่งพัฒนาโดยกรมวิทยาศาสตร์ กระทรวงสาธ

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