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Thèses sur le sujet « Enzyme-linked immunoassays »

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1

Carroll, Andrea D. "Development of bead injection methodology for immunoassays /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/8599.

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Kiote-Schmidt, Chrissoula. "Etablierung eines kompetitiven enzymgekoppelten Immunoassays zum Nachweis eines kleinen Peptids in Serum- und Liquorproben." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-59192.

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3

Booth, Ronald A. Carleton University Dissertation Biology. "Development of cloth-enzyme immunoassays for the detection of E. coli O157 and verotoxin." Ottawa, 1996.

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4

Schob, Stefan, Donald Lobsien, Benjamin Friedrich, et al. "The cerebral surfactant system and its alteration in hydrocephalic conditions." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-213883.

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Introduction: Pulmonary Surfactant reduces surface tension in the terminal airways thus facilitating breathing and contributes to host's innate immunity. Surfactant Proteins (SP) A, B, C and D were recently identified as inherent proteins of the CNS. Aim of the study was to investigate cerebrospinal fluid (CSF) SP levels in hydrocephalus patients compared to normal subjects. Patients and methods: CSF SP A-D levels were quantified using commercially available ELISA kits in 126 patients (0±84 years, mean 39 years). 60 patients without CNS pathologies served as a control group. Hydrocephalus pati
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5

Hardy, Charles Thomas. "Evaluation and calibration of enzyme immunoassays for detecting antibody to the human immunodeficiency virus and other agents /." Thesis, Connect to this title online; UW restricted, 1993. http://hdl.handle.net/1773/9297.

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6

von, Buttlar Heiner, Sabine Siegemund, Matthias Büttner, and Gottfried Alber. "Identification of toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells." Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-151442.

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Parapoxvirus ovis (PPVO) is known for its immunostimulatory capacities and has been successfully used to generate vector vaccines effective especially in non-permissive host species. Murine conventional and plasmacytoid dendritic cells (cDC and pDC) are able to recognize PPVO. The PPVO-sensing receptor on pDC is hitherto unknown. In this study we aimed to define the pattern recognition receptor responsible for the activation of murine pDC by inactivated and replication-competent PPVO. We show that PPVO-induced expression of type I and type III interferons, pro-inflammatory cytokines, and costi
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7

Orchard, Robert Graham. "A flow-through enzyme-linked immunoassay for progesterone." The University of Waikato, 2007. http://hdl.handle.net/10289/2277.

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Bovine reproductive performance is one of the most important factors influencing dairy farm profitability. Present-day techniques for oestrus- and pregnancy-detection are unreliable and labour-intensive. Although measuring milk-progesterone at regular intervals allows the fertility status of a cow to be determined reliably, the labour cost of collecting and analysing samples is prohibitive. This project aimed to develop a progesterone sensing system that could be automated and integrated with the milking unit, thus minimising labour costs. The proposed system involved mixing the milk sample wi
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8

Campbell, Charles Nelson. "Separationless immunoassay and DNA sensing using wired enzyme based amperometric affinity electrodes /." Digital version:, 2000. http://wwwlib.umi.com/cr/utexas/fullcit?p9992761.

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9

de, Alwis Wathuthanthirige Uditha. "Analytical applications of chemically modified antibodies." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184601.

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The components involved in an immunoassay were investigated in order to improve the detection limits of the ELISA and to make the assay adaptable to a flow injection analysis (FIA) configuration. The goal being the total automation of the ELISA procedure which is long, tedious and has high standard deviation. The antibody purification and cleavage methods were studied with special emphasis on obtaining products with highest immunological activity. The antibody-enzyme coupling reactions using homobifunctional reagents and heterobifunctional reagents were studied in order to attempt the preparat
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10

Lövgren, Ulf. "Enzyme immunoassay in combination with liquid chromatography for sensitive and selective determination of drugs in biosamples." Lund : Dept. of Analytical Chemistry, Lund University, 1997. http://books.google.com/books?id=Ju1qAAAAMAAJ.

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11

Tandy, Mark Wayne. "Early diagnosis of systemic Candida infection : development of an enzyme immunoassay for mycelial antigen and comparison with other methods." Thesis, Queensland University of Technology, 1986. https://eprints.qut.edu.au/36810/1/36810_Tandy_1986.pdf.

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An Enzyme immunoassay (ELISA) using monoclonal antibodies was developed to detect antigen associated with Candida albicans mycelial cytoplasm. One hundred and fourteen sera from patients with and at risk of invasive Candida infection were assayed by this and five other laboratory diagnostic techniques; Counterimmunoelectrophoresis, Latex agglutination for mannan antigen, Passive haemagglutination for mannan antibody, Serum arabinitol levels, and Serum arabinitol/creatinine ratios. Sensitivites of the five tests were 22%,11%,44%,89%,and 78% respectively. Sensitivity for the ELISA was 44%. Spec
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12

Dumenil, Troy. "The development of an enzyme immunoassay for the detection of equine serum amyloid-A." Thesis, Queensland University of Technology, 2001.

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13

McDuffee, Emily. "A new immunoassay for quantification of a novel cancer antigen in serum and immunostaining of carcinoma tissues and cultured cells revealing the antigenic cellular location." [Johnson City, Tenn. : East Tennessee State University], 2002. http://etd-submit.etsu.edu/etd/theses/available/etd-1106102-175606/unrestricted/McDuffeeE112102a.pdf.

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14

Moberg, Ylva. "Evaluation of an equine-optimized enzyme-linked immunosorbent assay for the determination of serum insulin in canine and feline samples." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-354036.

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Background: Insulin is an important hormone for glucose homeostasis. It is released from β-cells in the endocrine pancreas as a response to increased concentrations of plasma glucose. The major effect of insulin is the facilitation of cellular uptake and storage of glucose as glycogen. Insulinomas are tumours that produce excessive amounts of insulin resulting in hypoglycaemia. The condition has been observed in dogs and cats and is often malignant. One part of establishing the diagnosis is confirmation of elevated concentrations of insulin in a hypoglycaemic sample. Aim: The aim of this study
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15

Kim, Sangkyung. "Redox cycling for an in-situ enzyme labeled immunoassay on interdigitated array electrodes." Diss., Available online, Georgia Institute of Technology, 2004:, 2004. http://etd.gatech.edu/theses/available/etd-08192004-105039/unrestricted/kim%5Fsangkyung%5F200412%5Fphd.pdf.

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Thesis (Ph. D.)--Biomedical Engineering, Georgia Institute of Technology, 2005.<br>Hesketh, Peter, Committee Chair ; Edmondson, Dale, Committee Member ; Frazier, Albert, Committee Member ; Hunt, William, Committee Member ; Janata, Jiri, Committee Member. Includes bibliographical references.
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16

Bahlmann, Arnold. "Entwicklung und Validierung eines Enzyme-linked Immunosorbent Assays (ELISA) für die Quantifizierung von Carbamazepin in Abwasser, Oberflächenwasser und Trinkwasser." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16729.

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Ein kompetitiver ELISA (Enzyme-linked Immunosorbent Assay) für den Nachweis von Carbamazepin (CBZ) mit einer Bestimmungsgrenze von ca. 30 ng/L wurde entwickelt und validiert. Dieser in Gewässern häufig auftretende anthropogene Marker wurde anschließend in einer Vielzahl an Proben aus Abwässern, Oberflächengewässern und Trinkwässern nachgewiesen. Der ELISA zeigte eine exzellente Präzision und erbrachte in allen Matrizes geringfügig höhere Analysenergebnisse als die Referenzmethode HPLC-MS/MS. Die beständige Überbestimmung der CBZ-Konzentration in Höhe von ca. 7 % konnte auf die Präsenz von Ceti
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17

Rowland, Denise Phi-Phoung. "Evaluation of soluble fibrin as an early marker of thrombosis." Thesis, Queensland University of Technology, 1997.

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18

Nording, Malin. "Rapid sample preparation and bioanalytical techniques for efficient screening of organic pollutants in the environment." Doctoral thesis, Umeå universitet, Kemi, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-842.

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Large numbers of samples often need to be prepared and analysed in surveys of organic pollutants in the environment, but while the methods commonly used in such surveys can provide abundant detail they are generally costly, time-consuming and require large amounts of resources, so there is a need for simpler techniques. The work underlying this thesis assessed the potential utility of more convenient sample preparation and bioanalytical techniques for rapidly screening various environmental matrices that could be useful complements to higher resolution methods. Initially, the utility of a simp
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19

de, Oliveira Felipe Marques Souza. "Development and Application of Proximity Assays for Proteome Analysis in Medicine." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-334536.

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Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. In paper I, we present an adaptation of the solid-phase proximity ligation assay (SP-PLA) for the detection of post-translational modification of proteins (PTMs). The assay was adapted for
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20

Cole, Steven Christopher. "Application of polymerase chain reaction (PCR) and enzyme linked immunoassays for the identification of enterotoxigenic Escherichia coli bacteria in piglet feces and antibodies in pig serum." 2003. http://hdl.handle.net/1993/19841.

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21

Shoha, Louis M. "An immunoassay to detect antibodies against endodontic medicaments a thesis submitted in partial fulfillment ... endodontics /." 1989. http://books.google.com/books?id=KIc9AAAAMAAJ.

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22

Widmann, Andrea A. "Development of an enzyme immunoassay using whole plasma to determine progesterone concentrations during early pregnancy in the mare." Thesis, 1991. http://hdl.handle.net/1957/37384.

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