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Articles de revues sur le sujet « Enzyme-linked immunoassays »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 26 juillet 2025

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1

Bai J K, Sonia, N. Midhun, Chakrapani K V, Haripriya G, Chandra Kiran Venkata Sai Pathuri, and N. Surya Ramalakshmi. "Enzyme linked immunosorbantassay- lab diagnosis: A review." Indian Journal of Microbiology Research 8, no. 1 (2021): 10–14. http://dx.doi.org/10.18231/j.ijmr.2021.003.

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Immunoassays are generally antigen antibody analytical methods used for quantitative or for qualitative analysis. They are most commonly used for diagnostic purposes, drug monitoring in pharmacokinetic studies and in the quality control. ELISA is the most common used immunoassay where an enzyme is linked to detect the antibodies in the blood. ELISA tests are more accurate compared to other antibody-assays as it yields quantitative results. This review article describes ELISA and its applications, types and limitations.

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Azimi, Nooshin T., Fujiang Wen, Richard M. Lister, Dennis A. Chen, and Fred E. Lytle. "Enzyme-Linked Immunoassays Using Nanosecond Fluorometric Detection." Applied Spectroscopy 46, no. 6 (1992): 994–98. http://dx.doi.org/10.1366/0003702924124402.

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Nanosecond temporal resolution is combined with an enzyme-linked immunosorbent assay (ELISA) to improve the lower limit of detection for a plant virus, brome mosaic virus. The method uses alkaline phosphatase as the enzyme link and β-naphthyl phosphate as the substrate. Enzymatic activity produces the highly fluorescent tag β-naphthol. The 8.9-ns lifetime of the tag allows temporal discrimination against the assay blank, providing a 64× improvement in the detection limit as compared to a steady-state measurement, and a ∼4100× improvement over a standard ELISA method incorporating the chromogen

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Carr, Ronald I., Mervat Mansour, Damaso Sadi, Hana James, and John Verrier Jones. "A substrate amplification system for enzyme-linked immunoassays." Journal of Immunological Methods 98, no. 2 (1987): 201–8. http://dx.doi.org/10.1016/0022-1759(87)90006-8.

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Carr, Ronald I., Mervat Mansour, Damaso Sadi, Hana James, and John Verrier Jones. "A substrate amplification system for enzyme-linked immunoassays." Journal of Immunological Methods 103, no. 1 (1987): 157. http://dx.doi.org/10.1016/0022-1759(87)90259-6.

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Verrier Jones, J., M. Mansour, H. James, D. Sadi, and R. I. Carr. "A substrate amplification system for enzyme-linked immunoassays." Journal of Immunological Methods 118, no. 1 (1989): 79–84. http://dx.doi.org/10.1016/0022-1759(89)90056-2.

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Ehm, M., and A. Kappel. "Immunoassays for diagnosis of coagulation disorders." Hämostaseologie 30, no. 04 (2010): 194–201. http://dx.doi.org/10.1055/s-0037-1619055.

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SummaryImmunoassays play a pivotal role in the clinical laboratory. In the coagulation section of the laboratory, they are used as an aid for diagnosis of deep vein thrombosis or pulmonary embolism, thrombophilia screening, or detection of coagulation factor deficiencies, respectively. Enzyme-linked immunosorbent assay (ELISA) and latex agglutination immunoassay technologies are currently most widely used, while Luminescent Oxygen Channeling Immuno - assay (LOCI®) and other chemiluminescencebased immunoassays are emerging technologies for the coagulation laboratory. However, not all immunoassa

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Zhou, Lu, Yifan Liu, Yang Lu, et al. "Recent Advances in the Immunoassays Based on Nanozymes." Biosensors 12, no. 12 (2022): 1119. http://dx.doi.org/10.3390/bios12121119.

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As a rapid and simple method for the detection of multiple targets, immunoassay has attracted extensive attention due to the merits of high specificity and sensitivity. Notably, enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay, which can provide high detection sensitivity since the enzyme labels can promote the generation of catalytically amplified readouts. However, the natural enzyme labels usually suffer from low stability, high cost, and difficult storage. Inspired by the advantages of superior and tunable catalytic activities, easy preparation, low cost, and high sta

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Rajesh, K. Sharma, K. Sharma Pankaj, Talat Gaush, Gautam Praveen, Chhabra Reba, and Singh Surinder. "BRIEF OVERVIEW ON HEPATITIS C VIRUS IMMUNOASSAYS." International Journal of Research – Granthaalayah 4, no. 1 (2017): 178–84. https://doi.org/10.5281/zenodo.848525.

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The publication deals with a brief overview of Hepatitis C Virus (HCV) and donor blood screening for HCV by using conventional Rapid, Enzyme Linked Immunosorbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA) also. The advantages of various generation o

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Mutayoba, B. M., H. H. D. Meyer, D. Schams, and E. Schallenberger. "Development of a sensitive enzyme immunoassay for LH determination in bovine plasma using the streptavidin-biotin technique." Acta Endocrinologica 122, no. 2 (1990): 227–32. http://dx.doi.org/10.1530/acta.0.1220227.

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Abstract Using the biotin-streptavidin amplification technique, highly sensitive specific second-antibody enzyme immunoassays for determining LH in bovine plasma with long (48 h) and short (4 h) incubation periods were developed. Biotin was linked to bLH by the N-hydroxysuccimidine method and the product (biotinyl-bLH) used to bridge between streptavidin-peroxidase and the immobilised bLH antibody in competitive tests. The assays were validated and their performance compaired with a radioimmunoassay currently in use. The sensitivities of the long and short incubation enzyme immunoassays (8 pg

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Liu, Lin, Yuanqiang Hao, Dehua Deng, and Ning Xia. "Nanomaterials-Based Colorimetric Immunoassays." Nanomaterials 9, no. 3 (2019): 316. http://dx.doi.org/10.3390/nano9030316.

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Colorimetric immunoassays for tumor marker detection have attracted considerable attention due to their simplicity and high efficiency. With the achievements of nanotechnology and nanoscience, nanomaterials-based colorimetric immunoassays have been demonstrated to be promising alternatives to conventional colorimetric enzyme-linked immunoassays. This review is focused on the progress in colorimetric immunoassays with the signal amplification of nanomaterials, including nanomaterials-based artificial enzymes to catalyze the chromogenic reactions, analyte-induced aggregation or size/morphology c

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Laze, Blerta, and Anila Mitre. "Comparison of ECL, ELISA and ELFA immunoassays." Buletini Shkencor Reald 8, no. 1 (2023): 40–49. http://dx.doi.org/10.59858/bshr100043.

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The aim of these study was the evaluation of electrochemiluminescence assay (ECL), enzyme-linked immunosorbent assay (ELISA) and enzyme-linked fluorescent assay (ELFA), for an early diagnosis of Cytomegalovirus infections in pregnant women. In medical diagnostics, it is necessary to determine the most sensitive techniques for the detection of this pathogen, due to it’s multiple fetal infections during pregnancy. The ECL technique resulted with the highest sensitivity and specificity values. However, for diagnostic purposes, the results should always be assessed in conjunction with the patient’

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Liu, Lin, Yong Chang, Jiaxin Lou, Shuo Zhang, and Xinyao Yi. "Overview on the Development of Alkaline-Phosphatase-Linked Optical Immunoassays." Molecules 28, no. 18 (2023): 6565. http://dx.doi.org/10.3390/molecules28186565.

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The drive to achieve ultrasensitive target detection with exceptional efficiency and accuracy requires the advancement of immunoassays. Optical immunoassays have demonstrated significant potential in clinical diagnosis, food safety, environmental protection, and other fields. Through the innovative and feasible combination of enzyme catalysis and optical immunoassays, notable progress has been made in enhancing analytical performances. Among the kinds of reporter enzymes, alkaline phosphatase (ALP) stands out due to its high catalytic activity, elevated turnover number, and broad substrate spe

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Vítková, M., Z. Macková, R. Koblovská, and O. Lapčík. "Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants." Czech Journal of Food Sciences 22, SI - Chem. Reactions in Foods V (2004): S199—S202. http://dx.doi.org/10.17221/10659-cjfs.

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The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4’ via a carboxy methyl spacer. The developed ELISAs are highly specific, I<sub>50</sub> values of the standard curves range between 0.3–1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid

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Jeon, Hye-Bin, Dong-Yeon Song, Yu Jin Park, and Dong-Myung Kim. "Enhancing ELISA Sensitivity: From Surface Engineering to Synthetic Biology." Biosensors 15, no. 7 (2025): 434. https://doi.org/10.3390/bios15070434.

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Accurate and sensitive detection of protein biomarkers is critical for advancing in vitro diagnostics (IVD), yet conventional enzyme-linked immunosorbent assays (ELISA) often fall short in terms of sensitivity compared to nucleic acid-based tests. Bridging this sensitivity gap is essential for improving diagnostic accuracy, particularly in diseases where protein levels better reflect disease progression than nucleic acid biomarkers. In this review, we present strategies developed to enhance the sensitivity of ELISA, structured according to the sequential steps of the assay workflow. Beginning

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Sheng, Enze, Mei Du, Jiachuan Yang, Xiude Hua, and Minghua Wang. "Development of immunoassays for detecting oxyfluorfen residue in agricultural and environmental samples." RSC Advances 8, no. 9 (2018): 5020–25. http://dx.doi.org/10.1039/c7ra12445g.

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Uclés, Ana, Antonio Valverde García, María Dolores Gil García, Ana María Aguilera del Real, and Amadeo R. Fernández-Alba. "Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay." Analytical Methods 7, no. 21 (2015): 9158–65. http://dx.doi.org/10.1039/c5ay01048a.

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Kim, Dongwook, Rachael R. Jetson, and Casey J. Krusemark. "A DNA-assisted immunoassay for enzyme activity via a DNA-linked, activity-based probe." Chemical Communications 53, no. 68 (2017): 9474–77. http://dx.doi.org/10.1039/c7cc05236g.

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Pinsky, Norman A., Jeanne M. Huddleston, Robert M. Jacobson, Peter C. Wollan, and Gregory A. Poland. "Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies." Clinical Diagnostic Laboratory Immunology 10, no. 1 (2003): 19–21. http://dx.doi.org/10.1128/cdli.10.1.19-21.2003.

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ABSTRACT We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels

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Velázquez, Blanca, Hugo Massaldi, Julio Battistoni, and José A. Chabalgoity. "Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays." Clinical Diagnostic Laboratory Immunology 12, no. 5 (2005): 683–84. http://dx.doi.org/10.1128/cdli.12.5.683-684.2005.

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ABSTRACT Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.

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Orton, Susan M., Amy Peace-Brewer, John L. Schmitz, Kristie Freeman, William C. Miller, and James D. Folds. "Practical Evaluation of Methods for Detection and Specificity of Autoantibodies to Extractable Nuclear Antigens." Clinical Diagnostic Laboratory Immunology 11, no. 2 (2004): 297–301. http://dx.doi.org/10.1128/cdli.11.2.297-301.2004.

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ABSTRACT Detection and specificity of autoantibodies against extractable nuclear antigens (ENA) play a critical role in the diagnosis and management of autoimmune disease. Historically, the detection of these antibodies has employed double immunodiffusion (DID). Autoantibody specificity was correlated with diagnoses by this technique. Enzyme immunoassays have been developed by multiple manufacturers to detect and identify the specificity ENA autoantibodies. To address the relationship of ENA detection by DID and enzyme immunoassay, the performances of five immunoassays were compared. These inc

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McNulty, Melissa, Ravinder J. Singh, Xujian Li, Eric J. Bergstralh, and Rajiv Kumar. "Determination of Serum and Plasma Sclerostin Concentrations by Enzyme-Linked Immunoassays." Journal of Clinical Endocrinology & Metabolism 96, no. 7 (2011): E1159—E1162. http://dx.doi.org/10.1210/jc.2011-0254.

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Gómez-Sánchez, Celso E., Lissette M. León, and Elise P. Gómez-Sánchez. "Biotin-hydrazide derivatives for the development of steroid enzyme-linked immunoassays." Journal of Steroid Biochemistry and Molecular Biology 43, no. 6 (1992): 523–27. http://dx.doi.org/10.1016/0960-0760(92)90239-f.

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de la Rica, Roberto, and Aldrik H. Velders. "Supramolecular Au Nanoparticle Assemblies as Optical Probes for Enzyme-Linked Immunoassays." Small 7, no. 1 (2010): 66–69. http://dx.doi.org/10.1002/smll.201001340.

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Nestorova, Gergana G., Varun L. Kopparthy, Niel D. Crews, and Eric J. Guilbeau. "Thermoelectric lab-on-a-chip ELISA." Analytical Methods 7, no. 5 (2015): 2055–63. http://dx.doi.org/10.1039/c4ay02764g.

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Thermoelectric lab-on-a-chip ELISA is a novel method performing immunoassays by measuring the heat of the enzymatic reaction between enzyme-linked detection antibody and a substrate using a thin-film thermopile.

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Lyu, Zhaoyuan, Shichao Ding, Nan Zhang та ін. "Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of Aβ 1-40: A Biomarker of Alzheimer’s Disease". Research 2020 (19 жовтня 2020): 1–11. http://dx.doi.org/10.34133/2020/4724505.

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Single-atom nanozymes (SANs) possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity. By introducing SANs into immunoassay, limitations of ELISA such as low stability of horseradish peroxidase (HRP) can be well addressed, thereby improving the performance of the immunoassays. In this work, we have developed novel Fe-N-C single-atom nanozymes (Fe-Nx SANs) derived from Fe-doped polypyrrole (PPy) nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40. Result

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Sharma, Rajesh, Pankaj Sharma, Gaush Talat, Praveen Gautam, Reba Chhabra, and Surinder Singh. "BRIEF OVERVIEW ON HEPATITIS C VIRUS IMMUNOASSAYS." International Journal of Research -GRANTHAALAYAH 4, no. 1 (2016): 178–84. http://dx.doi.org/10.29121/granthaalayah.v4.i1.2016.2862.

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The publication deals with a brief overview of Hepatitis C Virus (HCV) and donor blood screening for HCV by using conventional Rapid, Enzyme Linked Immunosorbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA) also. The advantages of various generation of HCV tests in terms of sensitivity, specificity and reduction in window period are discussed.

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Bar-Haim, Erez, Shahar Rotem, Uri Elia, et al. "Early Diagnosis of Pathogen Infection by Cell-Based Activation Immunoassay." Cells 8, no. 9 (2019): 952. http://dx.doi.org/10.3390/cells8090952.

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Diagnostic identification of pathogens is usually accomplished by isolation of the pathogen or its substances, and should correlate with the time and site of infection. Alternatively, immunoassays such as enzyme-linked immunosorbent assay (ELISA) tests for quantification of serum antibodies are expedient and are usually employed for retrospective diagnostic of a particular infective agent. Here, the potential of cell-based immunoassays for early pathogen detection was evaluated by quantification of specific, antigen-activated, low-frequency IFNγ-secreting cells in mouse spleens following infec

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Banno, Yuto, Takuma Nomiyama, Shoma Okuno, Sachiko Ide, and Noritada Kaji. "Quantitative Evaluation of Interleukin-4 by Immunowall Devices Made of Gelatin Methacryloyl Hydrogel." Molecules 28, no. 12 (2023): 4635. http://dx.doi.org/10.3390/molecules28124635.

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Immunoassays, which use antigen–antibody reactions, are the primary techniques used to selectively quantify specific disease markers in blood. Conventional immunoassays, such as the microplate-based enzyme-linked immunosorbent assay (ELISA) and paper-based immunochromatography, are widely used, but they have advantages and disadvantages in terms of sensitivity and operating time. Therefore, in recent years, microfluidic-chip-based immunoassay devices with high sensitivity, rapidity and simplicity, which are compatible with whole blood assays and multiplex assays, have been actively investigate

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LaBrecque, F. D., D. R. LaBrecque, D. Klinzman, et al. "Recombinant Hepatitis A Virus Antigen: Improved Production and Utility in Diagnostic Immunoassays." Journal of Clinical Microbiology 36, no. 7 (1998): 2014–18. http://dx.doi.org/10.1128/jcm.36.7.2014-2018.1998.

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Hepatitis A virus (HAV) immunoassays use cell culture-derived HAV antigen to detect HAV-specific antibodies. The current method of production of HAV antigen in tissue culture is time-consuming and expensive. We previously expressed the HAV open reading frame in recombinant vaccinia viruses (rV-ORF). The recombinant HAV polyprotein was accurately processed and was assembled into subviral particles. These particles were bound by HAV-neutralizing antibodies and were able to elicit antibodies which were detected by commercial immunoassays. The present investigation compared the production of HAV a

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Butler, John E. "Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays." Methods 22, no. 1 (2000): 4–23. http://dx.doi.org/10.1006/meth.2000.1031.

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Xia, Ning, Fengli Gao, Jiwen Zhang, Jiaqiang Wang, and Yaliang Huang. "Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling." Molecules 29, no. 12 (2024): 2796. http://dx.doi.org/10.3390/molecules29122796.

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Enzyme-linked electrochemical immunosensors have attracted considerable attention for the sensitive and selective detection of various targets in clinical diagnosis, food quality control, and environmental analysis. In order to improve the performances of conventional immunoassays, significant efforts have been made to couple enzyme-linked or nanozyme-based catalysis and redox cycling for signal amplification. The current review summarizes the recent advances in the development of enzyme- or nanozyme-based electrochemical immunosensors with redox cycling for signal amplification. The special f

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Peuravuori, H., and T. Korpela. "Pyrophosphatase-based enzyme-linked immunosorbent assay of total IgE in serum." Clinical Chemistry 39, no. 5 (1993): 846–51. http://dx.doi.org/10.1093/clinchem/39.5.846.

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Abstract Human IgE was purified to near homogeneity by a two-step procedure consisting of immunoaffinity chromatography and high-performance liquid chromatography. Mouse hybridoma cell lines secreting antibodies against IgE were generated. Monoclonal and polyclonal antibodies were used to develop a "sandwich"-type ELISA for determining total IgE in human serum. Inorganic pyrophosphatase (EC 3.6.1.1), an enzyme having a high turnover number, was used as the label. The mean analytical recovery of our ELISA was 95.2% and the results showed good linear correlation with an established RIA of IgE. W

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Kunishima, ShinjI, Klyotaka Hayashi, Sentaro Kobayashi, Tomokl Naoe, and Ryuzo Ohno. "New enzyme-linked immunosorbent assay for glycocalicin in plasma." Clinical Chemistry 37, no. 2 (1991): 169–72. http://dx.doi.org/10.1093/clinchem/37.2.169.

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Abstract A new sandwich-type enzyme-linked immunosorbent assay for quantifying glycocalicin, a proteolytic fragment of platelet membrane glycoprotein Ib, is described. The assay is based on the use of two monoclonal antibodies raised against glycoprotein Ib and involves the avidin-biotin technique. The detection limit is 7 micrograms/L and the range of glycocalicin determined in plasma is 0.01 to 1 mg/L. Assay time is 2 h. The intra-assay CV ranged from 3.6% to 5.2%, the interassay CV from 5.4% to 8.0%. Analytical recovery of purified glycocalicin added to a plasma pool averaged 96%. In 36 hea

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Danilov, Sergei, Fran??oise Savoie, Bertrand Lenoir, et al. "Development of enzyme-linked immunoassays for human angiotensin I converting enzyme suitable for large-scale studies." Journal of Hypertension 14, no. 6 (1996): 719–27. http://dx.doi.org/10.1097/00004872-199606000-00007.

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Thijssen, J. H., W. G. Wood, A. C. Kessler, et al. "Multicenter evaluation of new enzyme-linked immunoassays of follitropin and lutropin in serum or plasma." Clinical Chemistry 37, no. 7 (1991): 1257–63. http://dx.doi.org/10.1093/clinchem/37.7.1257.

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Abstract Results from a multicenter evaluation of two new enzyme-linked immunosorbent assays [Enzymun-Test for follitropin (FSH) and lutropin (LH)] are presented and compared with results from 11 other commercial immunoassays, radioactive as well as nonradioactive. Enzymun-Test FSH and LH assays are suitable for automated systems and manual applications. The tests were reproducible (CV less than 5%), highly specific, and sensitive enough (less than 0.5 int. unit/L) to measure the hormones directly in almost all patients' samples, except for LH measurements in prepubertal children. We did not f

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Polson, R. J., J. G. Kenna, I. P. Shears, A. Bomford, and R. Williams. "Measurement of ferritin in serum by an indirect competitive enzyme-linked immunosorbant assay." Clinical Chemistry 34, no. 4 (1988): 661–64. http://dx.doi.org/10.1093/clinchem/34.4.661.

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Abstract This indirect competitive enzyme-linked immunosorbant assay (ELISA) for ferritin, unlike other currently available ELISAS, does not require use of an anti-ferritin antibody-enzyme conjugate. Designed for use on microtiter plates, the method has a precision and sensitivity similar to those of other immunoassays. The detection limit is 20 pg of ferritin per test (corresponding to 2.0 micrograms/L in serum samples). Comparison of results obtained on serum from 57 patients by this method with those from a conventional radioimmunoassay gave a correlation coefficient of 0.92.

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Huang, Rui-Lin, Yi-Chen Fu, Yung-Chih Wang, et al. "A Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader for SARS-CoV-2 Neutralizing Antibody Detection." Vaccines 10, no. 2 (2022): 271. http://dx.doi.org/10.3390/vaccines10020271.

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As of August 2021, there have been over 200 million confirmed case of coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus and more than 4 million COVID-19-related deaths globally. Although real-time polymerase chain reaction is considered to be the primary method of detection for SARS-CoV-2 infection, the use of serological assays for detecting COVID-19 antibodies has been shown to be effective in aiding with diagnosis, particularly in patients who have recovered from the disease and those in later stages of infection. Since it has a high detection rate and few lim

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Li, Li, Wenbo Lu, Chang Liu, Ying Wang, Jian Dong, and Weiping Qian. "Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres." Nano 12, no. 08 (2017): 1750092. http://dx.doi.org/10.1142/s1793292017500928.

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The sandwich-type immunoassays have been developed by using electrochemical and surface-enhanced Raman scattering (SERS) techniques for the detection of carcinoembryonic antigen (CEA). Nile blue as a kind of Raman dye has been decorated on nanospheres with polydopamine resin (PDR) via [Formula: see text]-stacking interaction. The Nile blue displays the strong SERS signals as well as a characteristic electrochemical reduction peak at [Formula: see text]0.33[Formula: see text]V (versus Ag/AgCl). The implementation of the PDR nanospheres mixing with Au nanoparticles (AuNPs/PDR) exhibits a better

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Radha, Remya, Syeda Kiran Shahzadi, and Mohammad Hussein Al-Sayah. "Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review." Molecules 26, no. 16 (2021): 4812. http://dx.doi.org/10.3390/molecules26164812.

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Cardiovascular diseases are considered one of the major causes of human death globally. Myocardial infarction (MI), characterized by a diminished flow of blood to the heart, presents the highest rate of morbidity and mortality among all other cardiovascular diseases. These fatal effects have triggered the need for early diagnosis of appropriate biomarkers so that countermeasures can be taken. Cardiac troponin, the central key element of muscle regulation and contraction, is the most specific biomarker for cardiac injury and is considered the “gold standard”. Due to its high specificity, the me

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Safenkova, Irina V., Pavel A. Galushka, Yuri A. Varitsev, et al. "Highly Targeted Detection of Priority Phytopathogen Pectobacterium brasiliense: From Obtaining Polyclonal Antibodies to Development and Approbation of Enzyme-Linked Immunoassay and Lateral Flow Immunoassay." Microorganisms 12, no. 12 (2024): 2436. http://dx.doi.org/10.3390/microorganisms12122436.

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Pectobacterium brasiliense is a bacterial phytopathogen that causes soft and black rot and actively spreads worldwide. Our study is the first development of immunoassays for detecting P. brasiliense. We immunized rabbits and obtained serum with an extremely high titer (1:108). Isolated polyclonal antibodies were tested by enzyme-linked immunosorbent assay (ELISA) using 18 closely related strains and 5 non-related bacterial pathogens. No cross-reactivity was found concerning the tested pathogens. The ELISA of P. brasiliense was developed in a double-antibody sandwich format with a detection lim

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Weiler, Barbara E., Heike Schäcke, Michael Bachmann, et al. "Human immunodeficiency virus: novel enzyme-linked immunoassays for quantitation of envelope glycoprotein 120." Journal of Virological Methods 32, no. 2-3 (1991): 287–301. http://dx.doi.org/10.1016/0166-0934(91)90059-9.

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Katlinskaya, Yuliya, Sviatlana Akalovich, Kanstantsin Katlinski, and Nikolai N. Voitenok. "Enzyme-linked immunoassays differentially recognizing glycosylated and deglycosylated forms of soluble human CXCR2." Cytokine 48, no. 1-2 (2009): 81–82. http://dx.doi.org/10.1016/j.cyto.2009.07.288.

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Terzapulo, Xeniya, Aiym Kassenova, and Rostislav Bukasov. "Immunoassays: Analytical and Clinical Performance, Challenges, and Perspectives of SERS Detection in Comparison with Fluorescent Spectroscopic Detection." International Journal of Molecular Sciences 25, no. 4 (2024): 2080. http://dx.doi.org/10.3390/ijms25042080.

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Immunoassays (IAs) with fluorescence-based detection are already well-established commercialized biosensing methods, such as enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA). Immunoassays with surface-enhanced Raman spectroscopy (SERS) detection have received significant attention from the research community for at least two decades, but so far they still lack a wide clinical commercial application. This review, unlike any other review that we have seen, performs a three-dimensional performance comparison of SERS IAs vs. fluorescence IAs. First, we compared the lim

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Khan, Imran H., Sara Mendoza, JoAnn Yee, et al. "Simultaneous Detection of Antibodies to Six Nonhuman-Primate Viruses by Multiplex Microbead Immunoassay." Clinical and Vaccine Immunology 13, no. 1 (2006): 45–52. http://dx.doi.org/10.1128/cvi.13.1.45-52.2006.

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ABSTRACT To maintain healthy nonhuman primates for use in biomedical research, animals are routinely screened for several infectious agents at most facilities. Commonly, monkey serum samples are tested by conventional immunoassays, such as enzyme-linked immunosorbent assays (ELISAs) or Western blotting, for antibodies to specific infectious agents. For testing for antibodies against multiple agents in each sample, conventional immunoassays are laborious and time-consuming. More efficient immunoassays are needed. Accordingly, we have developed a novel multiplex serodiagnostic system based on in

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Fan, Chenchen. "Comparative analysis of three immunoassays for SARS-CoV-2 detection." Highlights in Science, Engineering and Technology 99 (June 18, 2024): 88–94. http://dx.doi.org/10.54097/1ewjt996.

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As the 2019 coronavirus disease (COVID-19) spread rapidly around the world starting in late 2019, researchers were intrigued by the emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. In many nations, the real-time reverse transcription polymerase chain reaction (RT-PCR) is the accepted "gold standard" technique to detect COVID-19 because of its rapid process with high-level specificity. To solve the false-positive results caused by real-time RT-PCR test, more immunoassays in serological diagnosis need to be developed such as enzyme-linked immunosorbent assays (ELISA),

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Hirokawa, Shinichiro, and Eberhard F. Mammen. "A Functional Protein S and Microlatex Immunoassay for Protein S and C4b-Binding Protein on the Automated Coagulation Laboratory (ACL) 300 Plus." Clinical and Applied Thrombosis/Hemostasis 2, no. 4 (1996): 268–75. http://dx.doi.org/10.1177/107602969600200407.

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Protein S can be determined by functional or immunological assays. Electroimmunodiffusion (EID) or enzyme immunoassays (enzyme-linked immunosorbent assay; ELISA) are the commonly employed techniques for measuring protein S and C4b-binding protein (C4b- BP) immunologically. Procedures for these assays are time-consuming and labor-intensive. The introduction of microlatex immunoassays (LIATEST system; Diagnos tica Stago, Asnieres-Sur-Seine, France) has provided an alternative for rapid and reliable immunological determi nation. We have placed the microlatex immunoassay for total and free protein

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Kim, Jaehi, Min-Sup Shin, Jonghyun Shin, et al. "Recent Trends in Lateral Flow Immunoassays with Optical Nanoparticles." International Journal of Molecular Sciences 24, no. 11 (2023): 9600. http://dx.doi.org/10.3390/ijms24119600.

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Rapid, accurate, and convenient diagnosis is essential for effective disease management. Various detection methods, such as enzyme-linked immunosorbent assay, have been extensively used, with lateral flow immunoassay (LFIA) recently emerging as a major diagnostic tool. Nanoparticles (NPs) with characteristic optical properties are used as probes for LFIA, and researchers have presented various types of optical NPs with modified optical properties. Herein, we review the literature on LFIA with optical NPs for the detection of specific targets in the context of diagnostics.

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Mičková, B., P. Rauch, A. Montoya, E. Ferri, F. Fini, and S. Girotti. "The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection." Czech Journal of Food Sciences 22, SI - Chem. Reactions in Foods V (2004): S280—S282. http://dx.doi.org/10.17221/10681-cjfs.

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In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I<sub>50</sub> value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate conc

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Feuser, Paulo Emilio, Camila Guindani, Jonathann Correa Possato, et al. "Bovine Serum Albumin Conjugation in Superparamagnetic/Poly(methyl methacrylate) Nanoparticles as an Alternative for Magnetic Enzyme-Linked Immunosorbent Assays." Journal of Nanoscience and Nanotechnology 21, no. 11 (2021): 5493–98. http://dx.doi.org/10.1166/jnn.2021.19458.

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Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM)

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Wang, Bing, Jincui Gu, Boyi Chen, et al. "Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool." Journal of Analytical Methods in Chemistry 2018 (June 5, 2018): 1–9. http://dx.doi.org/10.1155/2018/2641624.

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The identification of ancient wool is of great importance in archaeology. Despite lots of meaningful information can be achieved by conventional detection methods, that is, light and electron microscopy, spectroscopy, and chromatography, the efficacy is likely to be limited in the detection of ancient samples with contamination or severe degradation. In this work, an immunoassay was proposed and performed for the identification of ancient wool. First, a specific antibody, which has the benefits of low cost, easy operation, and extensive applicability, was developed directly through immunizing

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