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Rozprawy doktorskie na temat „Grapes Genetics”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 30 stycznia 2023

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1

Ross-Adams, Helen Esther. "The characterisation of selected grapevine cultivars using microsatellites." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/53092.

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Thesis (MScAgric)--Stellenbosch University, 2002.<br>ENGLISH ABSTRACT: Grapevine supports one of the oldest industries in South Africa today, and is also of significant international importance. With increasing international trade and the transport of fruit and other grapevine-derived products between borders, it has become increasingly important for South African farmers and viticulturalists to ensure their products conform to strict international market requirements if they are to remain competitive. Such requirements include the correct and accurate identification of berries and wine

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2

Espach, Yolandi. "The detection of mycoviral sequences in grapevine using next-generation sequencing." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/80025.

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Thesis (MSc)--Stellenbosch University, 2013.<br>ENGLISH ABSTRACT: Metagenomic studies that make use of next-generation sequencing (NGS) generate large amounts of sequence data, representing the genomes of multiple organisms of which no prior knowledge is necessarily available. In this study, a metagenomic NGS approach was used to detect multiple novel mycoviral sequences in grapevine phloem tissue. Individual sequencing libraries of doublestranded RNA (dsRNA) from two grapevine leafroll diseased (GLD) and three shiraz diseased (SD) vines were sequenced using an Illumina HiScanSQ instrumen

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3

Venter, Mauritz. "Isolation of grapevine promoters with special emphasis on the vacuolar pyrophosphatase." Thesis, Stellenbosch : University of Stellenbosch, 2004. http://hdl.handle.net/10019.1/16078.

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Dissertation (PhD)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Understanding the complex nature of grapevine molecular biology is of great importance for viticulturists. Progress in the elucidation of key events on a genetic level could provide further insight into the underlying cues responsible for the precise control of physiological and metabolic changes during a specific condition such as fruit development. The use and analysis of molecular ‘tools’, such as promoters controlling the site and level of gene activity, could assist in the understanding of grapevine biology an

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4

Robson, Julia. "The construction of an expression vector for the transformation of the grape chloroplast genome." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53621.

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Thesis (MSc)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: The genetic information of plants is found in the nucleus, the mitochondria, and the plastids. The DNA of plastids is comprised of multiple copies of a double-stranded, circular, prokaryoticallyderived genome of -150 kb. The genome equivalents of plastid organelles in higher plant cells are an attractive target for genetic engineering as high protein expression levels are readily obtained due to the high genome copy number per organelle. The resultant proteins are contained within the plastid organelle and the correspon

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5

Olivier, Abraham Jacobus. "Differential gene expression during berry ripening in Vitis vinifera (cv Chardonnay) : isolation of specific sequences through subtractive cloning." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52802.

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Thesis (MSc)--Stellenbosch University, 2002.<br>ENGLISH ABSTRACT: Grapevine is worldwide an agronomically important crop. Traditionally selective breeding has been used to improve existing cultivars. In the last ten years, however, the advent of biotechnology has shortened these breeding programmes by producing transgenic grapevine. Because this new technology is aimed at the possible genetic manipulation of the ripening process in grape berries, it is important to elucidate all the mechanisms that may be involved in ripening. The aim of the present study was the identification of genes

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6

Rose, B. A. (Beverley Ann). "The characterisation and partial sequencing of the grapevine chloroplast genome." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53763.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: A number of proteins essential for the survival of a plant are encoded by the chloroplast genome. The characterization and sequencing of a number of algal and plant chloroplast genomes has facilitated researchers understanding of cellular functions and metabolism. Chloroplast DNA (cpDNA) has also been used to determine inter- and intraspecies evolutionary relationships and this organelle offers an alternative means of expressing foreign genes. Although a number of species' chloroplast genomes have been characterized and

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7

Burger, Anita L. "The isolation and characterisation of a developmentally-regulated gene from Vitis vinifera L. berries." Thesis, Stellenbosch : University of Stellenbosch, 2004. http://hdl.handle.net/10019.1/15938.

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Dissertation (PhD)--University of Stellenbosch, 2004.<br>152 Leaves printed single pages, preliminary pages i-xiv and 129 numberd pages. Includes bibliography. List of abbreviations.<br>ENGLISH ABSTRACT: Despite increased focus on ripening-related gene transcription in grapevine, and the large number of ripening-related cDNAs identified from grapes in recent years, the molecular basis of processes involved in grape berry ripening is still poorly understood. Moreover, little is known about the mechanisms involved in the ripening-related regulation of fruit-specific genes, since the isolatio

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8

Van, Straten Celene Debra. "The construction of plant expression vectors for the introduction of leafroll disease resistance in grapevine." Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51950.

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Thesis (MSc)--University of Stellenbosch, 2000.<br>ENGLISH ABSTRACT: Grapevine leafroll is one of the most damaging viral diseases that affect many viticultural regions of the world. Numerous reports over the last few years have associated closterovirus-like particles with leafroll disease. To date, eight serologically distinct closteroviruses have been isolated from leafroll infected vines, of which grapevine leafroll associated closterovirus-3 (GLRaV-3) is the best characterized. Virus resistance in transgenic plants based on the expression of a virusderived gene is known as pathogen

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9

Van, Eeden C. (Christiaan). "The construction of gene silencing transformation vectors for the introduction of multiple-virus resistance in grapevines." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53764.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Viruses are some of the most important pathogens of grapevines. There are no effective chemical treatments, and no grapevine- or other natural resistance genes have been discovered against grapevine infecting viruses. The primary method of grapevine virus control is prevention by biological indexing and molecular- and serological screening of rootstocks and scions before propagation. Due to the spread of grapevine viruses through insect vectors, and in the case of GRSPaV the absence of serological screening, these metho

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10

Brackenridge, Anika Elma. "Over-expression and analysis of two Vitis vinifera carotenoid biosynthetic genes in transgenic Arabidopsis." Thesis, Stellenbosch : University of Stellenbosch, 2006. http://hdl.handle.net/10019/508.

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11

Taylor, Kerry Lyn. "Isolation and characterisation of carotenoid biosynthetic genes from Vitis vinifera." Thesis, Link to online version, 2007. http://hdl.handle.net/10019/469.

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12

Blignaut, Marguerite. "The molecular and biological characterisation of ORF5 of three South African variants of Grapevine Vitivirus A." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/2421.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2009.<br>Grapevine Vitivirus A (GVA), genus Vitivirus, family Flexiviridae is a well characterised single-stranded RNA virus that has been implicated in the grapevine diseases, Kober stem grooving and Shiraz disease. The virus infects both its host, Vitis vinifera and the experimental model plant, Nicotiana spp.. Biological studies performed on the virus in its herbaceous host, Nicotiana benthami- ana, revealed that many divergent variants of the virus exists in South Africa and can induce di erent symptoms in the model plant. Further

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13

Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ﬁcus (Signoret)". Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/4083.

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Thesis (MSc (Genetics))--Stellenbosch University, 2008.<br>The vine mealybug, Planococcus ficus (Signoret), is a severe pest of grapevine in many grape and wine producing countries around the world. It is renowned not only for the considerable damage it infers to grapevine of its own accord, but in particular for its role in transmitting deleterious viral diseases such as grapevine leafroll disease, Kober stem grooving, Shiraz disease and corky bark. Incidentally, it is an exceptionally tenacious antagonist of grapevine, being resistant to both chemical and biological control mechanisms.

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14

De, Koker Wenhelene Crystal. "Molecular characterization of grapevine virus E in South Africa." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71709.

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Thesis (MSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Grapevine virus E (GVE) is a newly identified virus that has been detected in an established vineyard in South Africa. This virus is a member of the genus Vitivirus, family Flexiviridae. Members of this genus are known to infecte grapevine and are associated with various disease complexes, such as the Rugose wood complex (RWC) and Shiraz disease (SD). However, the role and impact of GVE in South African vineyards are still unknown. It is important to study these viruses to determine how they infect and the possible impact they m

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15

Du, Preez Jacques. "The construction of an infectious clone of grapevine virus A (GV A)." Thesis, Link to the online version, 2005. http://hdl.handle.net/10019/1012.

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16

Carstens, Roleen. "The incidence and distribution of grapevine yellows disease in South African vineyards." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86683.

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Thesis (MSc)--Stellenbosch University, 2014.<br>ENGLISH ABSTRACT: South Africa is ranked eighth in the world as far as international wine production is concerned and in terms of area under bearing vines South Africa is ranked 12th. In 2011 the wine industry contributed R4 204.4 million to the South African economy in state revenue from wine products. The importance of viticulture to the economy of South Africa forces the industry to limit the effect of all disease causing pathogens in order to keep their competitive edge. Aster yellows (AY) phytoplasma 16SrI-B subgroup was reported for th

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17

Young, Philip Richard 1973. "Molecular analyses of candidate carotenoid biosynthetic genes in Vitis vinifera L." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53752.

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Thesis (PhD)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Plants cannot avoid stress and must therefore be capable of rapidly responding to extreme environmental changes. An inability to control and regulate the photosynthetic process during stress conditions will lead to the formation of highly reactive oxygen species that concomitantly causes photo-oxidative damage to the pigments and proteins of the photosynthetic apparatus. Since light is the primary source of energy for the photosynthetic process, it is clear that plants are continuously required to balance the light ene

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18

Bester, Rachelle. "Sequencing and detection of a new strain of grapevine leafroll-associated virus 3 in South Africa." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71743.

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Thesis (MSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Grapevine leafroll-associated virus 3 (GLRaV-3) is the type member of the genus Ampelovirus in the family Closteroviridae and is considered to be the main contributing agent of grapevine leafroll disease (GLD) worldwide. A metagenomic sequencing study of a grapevine leafroll-diseased vineyard led to the discovery of a new variant of GLRaV-3 in South Africa. This new variant was most related to a New Zealand isolate, NZ-1. In this study, we sequenced two isolates, GH11 and GH30, of the new variant group of GLRaV-3. These isolates

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19

Malan, Stefanie. "Real time PCR as a versatile tool for virus detection and transgenic plant analysis." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/1921.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2009.<br>ENGLISH ABSTRACT: South Africa is regarded as one of the top wine producing countries in the world. One of the threats to the sustainability of the wine industry is viral diseases of which Grapevine leafroll-associated virus 3 (GLRaV-3) and Grapevine virus A (GVA) are considered to be the most important and wide spread. Scion material is regularly tested for viruses; however scion material is often grafted onto rootstocks that have questionable phytosanitary status. Virus detection in rootstocks is challenging due to low and v

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20

Liebenberg, Annerie. "The development of an enzyme linked immunosorbent assay for the detection of the South African strain(s) of grapevine fanleaf nepovirus." Thesis, Link to the online version, 2008. http://hdl.handle.net/10019/1909.

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21

Visser, Marike. "An evaluation of the efficacy of antimicrobial peptides against grapevine pathogens." Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/6729.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2011.<br>Includes bibliography<br>ENGLISH ABSTRACT: This study investigated the use of antimicrobial peptides (AMPs) as possible source of resistance against a range of pathogens in grapevine. Whilst the ultimate aim would be to express AMPs in grapevine, the development of transgenic grapevine is time consuming and therefore pre-screening of potential AMPs is necessary. These small molecules, of less than 50 amino acids in length, are expressed by almost all organisms as part of their non-specific defence system. In vitro pre-screening of A

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22

Venter, Alida. "The functional analysis of Vitaceae polygalacturonase-inhibiting protein (PGIP) encoding genes overexpressed in tobacco." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4350.

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Thesis (MScAgric (Viticulture and Oenology. Wine Biotechnology))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Agriculture worldwide is under great pressure to produce enough food in order to sustain the ever-growing world population. Among the many challenges faced by food producers, crop losses and damage caused by fungal plant pathogens is a major problem. The study of fungal pathogens and the interaction between plants and fungi is therefore essential, and has been carried out for many years. Much has been learned in this time, but the full mechanisms of the various modes of

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23

Coetzee, Beatrix. "A metagenomic approach using next-generation sequencing for viral profiling of a vineyard and genetic characterization of grapevine virus E." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5186.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2010.<br>Includes bibliography.<br>Title page: Dept. of Genetics, Faculty of Science<br>ENGLISH ABSTRACT: Next-generation sequencing technologies are increasingly used in metagenomic studies, largely due to the high sequence data throughput capacity and unbiased approach in determining the genetic composition of an unknown environmental sample. This study investigated the applicability of the Illumina next-generation sequencing platform for metagenomic sequencing of grapevine viruses to provide the first complete viral profile, or virome

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24

Maree, Hans Jacob. "Identification and characterisation of grapevine leafroll-associated virus 3 genomic and subgenomic RNAs." Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5417.

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Thesis (PhD (Genetics))--University of Stellenbosch, 2010.<br>Includes bibliography.<br>Title page: Dept. of Genetics, Faculty of Science<br>ENGLISH ABSTRACT: Grapevine leafroll-associated virus 3 (GLRaV-3) is the type strain for the genus Ampelovirus, family Closteroviridae. There has been only one report that claimed the complete nucleotide sequence of GLRaV-3 (isolate NY-1, AF037268). Here we report the complete sequence of the South African GLRaV-3, isolate GP18 (EU259806) and show a significantly extended 5’ end. We used RLM-RACE to determine the 5’ end of GP18 and found the 5’ UTR t

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25

Scheper, Reiny W. A. "Studies on the biology and genetic variation of phomopsis on grapevine /." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phs325.pdf.

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Noach, Liesl Christine. "The molecular characterization of South African isolates of Grapevine Rupestris Stem Pitting-associated virus (GRSPaV)." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5252.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2010.<br>Includes bibliography.<br>ENGLISH ABSTRACT: The first aim of this study was to reliably and rapidly detect Grapevine rupestris stem pittingassociated virus (GRSPaV) in grapevine. This was achieved by screening 94 grapevines using crude plant extracts in both quantitative and conventional reverse transcription polymerase chain reaction (RT-PCR). The second aim was to establish a technique capable of differentiating GRSPaV sequence variants. The application of this technique is for the largescale screening of diseased vines to as

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27

Vaidya, Bijayeswar. "Genetics of autoimmune endocrine disorders." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341439.

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Sutherland, Catherine M. "A genetic strategy to reduce sulfite reductase activity in Saccharomyces cerevisiae /." Title page, contents and summary only, 2000. http://web4.library.adelaide.edu.au/theses/09APSP/09apsps966.pdf.

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29

Soloki, Mahmod. "Genetic transformation of grape somatic embryos." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387659.

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Uduman, Mohamed. "Identifying the largest complete data set from ALFRED /." Link to online version, 2006. https://ritdml.rit.edu/dspace/handle/1850/1876.

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Joubert, Dirk Albert 1973. "Regulation of the Vitis vinifera PGIP1 gene encoding a polygalacturonase-inhibiting protein." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53759.

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Thesis (PhD)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Plant-pathogen interactions have been intensively investigated in the last decade. This major drive towards understanding the fundamental aspects involved in plant disease resistance is propelled by the obvious agricultural and economical benefits that are intrinsically linked to disease and stress resistant plants. It is, therefore, not surprising that fundamental research in this area is not just restricted to model organisms, such as Arabidopsis and tobacco, but also extends to more traditional crop plants, such as

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32

Heward, Joanne Marie. "Genetic susceptibility to the development of Graves' disease." Thesis, University of Birmingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343429.

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33

Alabi, Olufemi Joseph. "Studies on epidemiology, molecular detection and genetic diversity of selected viruses infecting cassava and wine grapes." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Dissertations/Fall2009/o_alabi_110409.pdf.

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Minichiello, Mark Joseph. "Analysis of genetic variation data using ancestral recombination graphs." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613255.

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35

Santos, Roberto Bernardo dos. "Perfil genetico de risco para doença de Graves e para a oftalmopatia de Graves em uma população brasileira." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310274.

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Orientador: Laura Sterian Ward<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas<br>Made available in DSpace on 2018-08-13T00:14:50Z (GMT). No. of bitstreams: 1 Santos_RobertoBernardodos_D.pdf: 2009971 bytes, checksum: 0cf90a1782f718e3576f9172bd717ecf (MD5) Previous issue date: 2009<br>Resumo: A Doença de Graves é um processo imunológico em que a combinação de fatores genéticos e ambientais é fundamental. Vários genes têm sido propostos como envolvidos no desenvolvimento da doença, particularmente os genes do sistema HLA e os polimorfismos do gene CTLA-4.

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36

Samarakoon, Mudiyanselage Buddhika. "Design evolution of engineering systems using bond graphs and genetic programming." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/41900.

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Engineering design is a complex task, which typically involves multiple physical domains. It can benefit from a modeling tool that can represent different domains in a unified manner. For complex designs, optimization by traditional techniques (such as gradient-based methods) may not be appropriate. Evolutionary methodologies may be used in design optimization of complex engineering systems. This research is based on a framework for evolutionary design system consisting of a machine health monitoring system, model-based evolutionary design optimization system, and a design expert system. The d

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Levin, Alex Ph D. (Alexander) Massachusetts Institute of Technology. "Graphs, matrices, and populations : linear algebraic techniques in theoretical computer science and population genetics." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/83695.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Department of Mathematics, 2013.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 149-155).<br>In this thesis, we present several algorithmic results for problems in spectral graph theory and computational biology. The first part concerns the problem of spectral sparsification. It is known that every dense graph can be approximated in a strong sense by a sparse subgraph, known as a spectral sparsifier of the graph. Furthermore, researchers have recently developed efficient algorithms for computing suc

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38

Weninger, Timothy Edwards. "Link discovery in very large graphs by constructive induction using genetic programming." Thesis, Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/1087.

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Dockrall, Samantha. "Carotenoid cleavage dioxygenases (CCDs) of grape." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71899.

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Thesis (MScAgric)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Plant carotenoid cleavage dioxygenases (CCD) are a family of enzymes that catalyse the oxidative cleavage of carotenoids and/or apocarotenoids. Carotenoids are synthesised in plastids (primarily chloroplasts and chromoplasts), where they are involved in light-harvesting and protecting the photosynthetic apparatus from photo-oxidation. The carotenoid-derived apocarotenoids fulfil a number of roles in plants such as phytohormones, pollinator attractants and flavour and aroma compounds. Due to the floral and fruity characteris

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40

Sato, Akihiko. "Statistical genetic analysis of berry flesh texture and breeding strategies for its improvement in interspecific hybrid grapes." Kyoto University, 2006. http://hdl.handle.net/2433/144077.

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Kyoto University (京都大学)<br>0048<br>新制・論文博士<br>博士(農学)<br>乙第11839号<br>論農博第2593号<br>新制||農||928(附属図書館)<br>学位論文||H18||N4151(農学部図書室)<br>24381<br>UT51-2006-J536<br>(主査)教授米森敬三, 教授矢澤進, 教授谷坂隆俊<br>学位規則第4条第2項該当

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41

Forneck, Astrid. "Genetic variation and differences in host performance of grape phylloxera (Daktulosphaira vitifoliae Fitch) /." Stuttgart : Grauer, 1999. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009158066&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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42

Dickinson, Stephen John. "A building heating system simulation and optimisation tool incorporating bond graphs and genetic algorithms." Thesis, Lancaster University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.481623.

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43

Newton, Matthew. "Sequential and parallel algorithms for low-crossing graph drawing." Thesis, Loughborough University, 2007. https://dspace.lboro.ac.uk/2134/12944.

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The one- and two-sided bipartite graph drawing problem alms to find a layout of a bipartite graph, with vertices of the two parts placed on parallel imaginary lines, that has the minimum number of edge-crossings. Vertices of one part are in fixed positions for the one-sided problem, whereas all vertices are free to move along their lines in the two-sided version. Many different heuristics exist for finding approximations to these problems, which are NP-hard. New sequential and parallel methods for producing drawings with low edgecrossings are investigated and compared to existing algorithms, n

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Wilsen, Kathleen L. (Kathleen Lucy). "Investigating the introduction of a broadspectrum antiviral mechanism into grapevine." Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51803.

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Thesis (MSc)--University of Stellenbosch, 2000.<br>ENGLISH ABSTRACT: Ribosome inactivating proteins (RIPs) are potent toxins produced by a wide range of evolutionarily diverse plants. These toxins cause cell death by physically dismantling ribosomal RNA and shutting down protein synthesis. They also have a strong antiviral activity. Some believe that the antiviral property of RIPs is a function of ribosomal inactivation, others believe that the two properties are unrelated. RIPs are non-specific in their antiviral activity. Transgenic RIPexpressing plants are resistant to a wide spectru

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Bedin, Márcia Regina. "Associação dos polimorfismos -318C/T, CT60 e A49G do gene CTLA4, R620W do gene PTPN22 e A946T do gene IFIH1 em pacientes pediátricos com doença autoimune tireoidiana e diabetes mellitus tipo 1." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-27092013-123929/.

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As doenças autoimunes da tireoide (DAIT) representadas, principalmente pela doença de Graves (DG) e tireoidite de Hashimoto (TH), apresentam causas multifatoriais, incluindo fatores genéticos e ambientais. Diversos genes estão envolvidos, entre eles CTLA4, PTPN22 e mais recentemente IFIH1, principalmente quando associados a diabetes mellitus tipo 1 (DM1). OBJETIVOS: Determinar a frequência alélica e genotípica dos polimorfismos: -318C/T, A49G e CT60 do CTLA4, R620W do PTPN22 e A946T do IFIH1 em pacientes pediátricos portadores de DG, TH e DM1 associado a TH e em uma população controle normal,

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Zhai, Jing, Chiu-Hsieh Hsu, and Z. John Daye. "Ridle for sparse regression with mandatory covariates with application to the genetic assessment of histologic grades of breast cancer." BIOMED CENTRAL LTD, 2017. http://hdl.handle.net/10150/622811.

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Background: Many questions in statistical genomics can be formulated in terms of variable selection of candidate biological factors for modeling a trait or quantity of interest. Often, in these applications, additional covariates describing clinical, demographical or experimental effects must be included a priori as mandatory covariates while allowing the selection of a large number of candidate or optional variables. As genomic studies routinely require mandatory covariates, it is of interest to propose principled methods of variable selection that can incorporate mandatory covariates. Method

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Lorins, Peterson Marthen. "A Comparative Analysis Between Context-Based Reasoning (CxBR) and Contextual Graphs (CxGs)." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2302.

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Context-based Reasoning (CxBR) and Contextual Graphs (CxGs) involve the modeling of human behavior in autonomous and decision-support situations in which optimal human decision-making is of utmost importance. Both formalisms use the notion of contexts to allow the implementation of intelligent agents equipped with a context sensitive knowledge base. However, CxBR uses a set of discrete contexts, implying that models created using CxBR operate within one context at a given time interval. CxGs use a continuous context-based representation for a given problem-solving scenario for decision-support

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Francisco, Daniela Oliveira. "Aplicação de algoritmos bio-inspirados ao problema de geração automática de grades horárias." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/18/18153/tde-26082013-104459/.

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A geração de grades horárias de qualidade é um fator crítico em qualquer instituição de ensino, tanto em escolas de ensino fundamental/médio como em universidades. Este problema é considerado complexo, pois devem ser relacionados e otimizados diversos recursos, tais como horários, disciplinas, professores e alunos. Em grande parte das instituições de ensino, a geração de grades horárias é realizada manualmente, o que vem a tornar este processo custoso e sujeito a falhas. Diversas abordagens são também encontradas na literatura para resolução deste problema, nas quais foram aplicados métodos de

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Santibanez, Claudia. "Comparative genetic and metabolic characterization between two table grape varieties with contrasted color berry skin : red Globe and Chimenti Globe." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0918/document.

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Le développement de la baie de raisin est un processus dynamique présentant une courbe de croissance sigmoïde avec deux phases de croissance séparée par une phase de latence. Il se caractérise par une biosynthèse coordonnée de métabolites primaires et secondaires. À la fin de la phase de latence, un phénomène appelé véraison se produit, au cours duquel le fruit commence à prendre de la couleur et le processus de maturation est initié. Les anthocyanes sont responsables de la coloration des baies et la régulation de leur biosynthèse été largement étudiée. Cependant, peu d'études ont porté sur la

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Du, Preez Jacques. "The development and characterisation of grapevine virus-based expression vectors." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4003.

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Thesis (PhD (Genetics))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Grapevine (Vitis vinifera L.) is a very important agricultural commodity that needs to be protected. To achieve this several in vivo tools are needed for the study of this crop and the pathogens that infect it. Recently the grapevine genome has been sequenced and the next important step will be gene annotation and function using these in vivo tools. In this study the use of Grapevine virus A (GVA), genus Vitivirus, family Flexiviridae, as transient expression and VIGS vector for heterologous protein expression

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