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Artykuły w czasopismach na temat „In Vitro Cytotoxicity Test”

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Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Eskov, Andrey, Rinat Kayumov, and Anatoly Sokolov. "Dynamic, label free test for in vitro cytotoxicity." Toxicology Letters 211 (June 2012): S148. http://dx.doi.org/10.1016/j.toxlet.2012.03.539.

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Honma, Masamitsu. "Cytotoxicity measurement in in vitro chromosome aberration test and micronucleus test." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 724, no. 1-2 (2011): 86–87. http://dx.doi.org/10.1016/j.mrgentox.2011.05.003.

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Groth, Thomas, Peter Falck, and Rainer-Reginald Miethke. "Cytotoxicity of Biomaterials — Basic Mechanisms and In Vitro Test Methods: A Review." Alternatives to Laboratory Animals 23, no. 6 (1995): 790–99. http://dx.doi.org/10.1177/026119299502300609.

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Biocompatibility is one of the main prerequisites for the clinical use of biomaterials. Central to the testing of biocompatibility is the estimation of cytotoxicity, which can be assessed in vitro by using a variety of different target primary cells or cell lines. The influence of toxic agents derived from biomaterials on cellular functions and cell viability can be characterised by reductions in cell adhesion, alterations in cellular morphology, reduced cellular proliferation, and cell death, demonstrated by an absence of metabolic activity, structural disintegration and cell lysis. A brief r

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4

Spielmann, Horst, Manfred Liebsch, Sabine Kalweit, et al. "Results of a Validation Study in Germany on Two in Vitro Alternatives to the Draize Eye Irritation Test, the HET-CAM Test and the 3T3 NRU Cytotoxicity Test." Alternatives to Laboratory Animals 24, no. 5 (1996): 741–858. http://dx.doi.org/10.1177/026119299602400511.

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During 1988–1992, a validation study was carried out in Germany on the capacity of two in vitro tests to replace the Draize eye test for severely eye irritating chemicals, namely, the hen's egg chorio-allantoic membrane (HET-CAM) test and the 3T3 cell neutral red uptake (NRU) cytotoxicity test, which had shown promising results in an earlier test development project. The formal validation study, which was coordinated by Centre for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET) and funded by the German Department of Research and Technology (BMBF), was conducte

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5

Seibert, Hasso, Michael Gulden, Marike Kolossa, and Gabi Schepers. "Evaluation of the Relevance of Selected In Vitro Toxicity Test Systems for Acute Systemic Toxicity." Alternatives to Laboratory Animals 20, no. 2 (1992): 240–45. http://dx.doi.org/10.1177/026119299202000211.

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Progress in the implementation of in vitro methods in testing procedures for non-genetic toxicity is limited by poor or missing validation of the various approaches. Many efforts have focused on determinations of general cytotoxicity in vitro using proliferating, non-differentiated cell lines. The aim of our present studies is to evaluate the relevance to acute systemic toxicity of three in vitro systems using differentiated mammalian cells (bovine spermatozoa, primary cultured rat hepatocytes, primary cultured rat skeletal muscle cells) and also one in vitro system which uses a continuous cel

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6

Kumar, M. Praveen, Shravan Kini, M. S. Prathap, Aravind R. Kudva, Aboobacker Sidheeque, and Ashwini Prabhu. "In vitro cytotoxicity evaluation of endodontic irrigants combined with cationic peptide." Journal of Conservative Dentistry and Endodontics 28, no. 1 (2025): 84–89. https://doi.org/10.4103/jcde.jcde_690_24.

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Abstract Aim: The aim of this study was to assess the cytotoxicity of endodontic irrigants 2% chlorhexidine (CHX) and ethylenediaminetetraacetic acid (EDTA) combined with cationic peptide DJK-5 on L929 mouse subcutaneous fibroblast cells. Subjects and Methods: L929 cells were cultured and treated with 2% CHX, 2% CHX + DJK-5, 8.5% EDTA, 8.5% EDTA + DJK-5, and DJK-5 alone (DJK-5 peptide concentration–10 μg/mL) for 24 and 48 h. The cytotoxicity of the endodontic irrigants was determined using methyl thiazolyl tetrazolium assay and Live/Dead assay using acridine orange and ethidium bromide dual fl

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7

Srivastava, Sabita, Stephen D. Gorham, and James M. Courtney. "Screening of in vitro cytotoxicity by the adhesive film test." Biomaterials 11, no. 2 (1990): 133–37. http://dx.doi.org/10.1016/0142-9612(90)90129-e.

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8

Fang-gang, Liao, and Wei Yi. "Cytotoxicity test of nickel-containing ceramic metal alloys in vitro." Journal of Dentistry 19, no. 2 (1991): 132. http://dx.doi.org/10.1016/0300-5712(91)90112-c.

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9

MAZLUMOGLU, Busra Sahin. "In Vitro Cytotoxicity Test Methods: MTT and Neutral Red Uptake." Pharmata 3, no. 2 (2023): 50–53. http://dx.doi.org/10.5152/pharmata.2023.1287964.

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Hanan, M. Ali, Saad Abdul Samad Baryham, Loay Mohammed Noha, Shaheen Hammadi Saad, and Salim Temma Asawer. "Analytical study and cytotoxicity test of azo dye in vitro." Journal of Wildlife and Biodiversity 7, Special Issue (2023): 471–77. https://doi.org/10.5281/zenodo.10246975.

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Analytical study of the azo dye (HA) showed that it can be affected by their solubility and the dielectric constant (D) of solvents. The MTT assay was used to test the effectiveness of HA against MCF-7 cell line, which indicated their sensible inhibition rates in contrast with the control (C). The assay also showed the great ability of the (HA) azo dye in destroying the MDA-MB231 cells and how it affected their viability%, which was better than their ability against the MCF-7 cell line. And this activity increased with the increase of their concentration. Statistical analysis gave P<0.05. F

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11

Hunt, Stephen M., Christina Chrzanowska, Christopher R. Barnett, Helen N. Brand, and John K. Fawell. "A Comparison of In Vitro Cytotoxicity Assays and Their Application to Water Samples." Alternatives to Laboratory Animals 15, no. 1 (1987): 20–29. http://dx.doi.org/10.1177/026119298701500104.

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A group of 13 compounds were tested for in vitro cytotoxicity in four test systems; MIT-24 test, inhibition of cell growth (protein method), inhibition of cell growth (vital dye method) and cloning efficiency. In general, all four assays tended to rank compounds in a similar order for toxicity. The length of the exposure period appeared to be important for some compounds. The cytotoxicity of a variety of water samples was examined in two tests; inhibition of cell growth (vital dye method) and cloning efficiency. Under the conditions in which the assays were carried out, the latter proved to be

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12

Huang, Zehao, Na Li, Kaifeng Rao, Cuiting Liu, Zijian Wang, and Mei Ma. "In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests." Combinatorial Chemistry & High Throughput Screening 21, no. 4 (2018): 262–70. http://dx.doi.org/10.2174/1386207321666180330120248.

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Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations. Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China. Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micr

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13

Vojtassak, J., J. Carsky, and D. Böhmer. "Cytotoxicity test and cytogenetic analysis of effects of aminoguanidine in vitro." Methods and Findings in Experimental and Clinical Pharmacology 25, no. 1 (2003): 11. http://dx.doi.org/10.1358/mf.2003.25.1.772542.

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14

Clothier, Richard H., Lesley Hulme, Aamer B. Ahmed, Helen L. Reeves, Moira Smith, and Michael Balls. "In Vitro Cytotoxicity of 150 Chemicals to 3T3-L1 Cells, Assessed by the FRAME Kenacid Blue Method." Alternatives to Laboratory Animals 16, no. 1 (1988): 84–95. http://dx.doi.org/10.1177/026119298801600116.

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The cytotoxicities to 3T3-L1 cells of 150 coded chemicals, estimated by the FRAME kenacid blue method, are given as a basis for further studies on the relative in vitro toxicities of related and distinct sets of test chemicals, and as a basis for future attempts at the comparison and correlation of estimates of toxicity obtained in vitro and in vivo. The expression of the results of in vitro cytotoxicity tests is discussed, as are various ways of comparing values obtained for different chemicals. It is suggested that there should be agreement on the inclusion of a standard set of reference che

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15

Flint, Oliver P. "In Vitro Toxicity Testing: Purpose, Validation and Strategy." Alternatives to Laboratory Animals 18, no. 1_part_1 (1990): 11–18. http://dx.doi.org/10.1177/026119299001800103.1.

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The fullest potential for in vitro evaluation of toxicity will be realised in the context of the process of assessing the risk of human toxicity. This article is an attempt to clarify what contributions can be made by in vitro tests and what types of in vitro test can best be used. In vitro tests are clarified according to the type of biological endpoint evaluated, first into tests for general (‘basal’) cytotoxicity and, secondly, into tests for differentiated cell function. The role of each type of test is analysed and it is suggested that tests for general cytotoxicity, as opposed to differe

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16

Cahours, X., M. Blanchet, M. Dubois, and C. Haond. "Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke." Beiträge zur Tabakforschung International/Contributions to Tobacco Research 24, no. 4 (2011): 157–65. http://dx.doi.org/10.2478/cttr-2013-0893.

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AbstractCurrently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test). These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to the preparation and the handling of cells. It is difficult to use these in vitro tests as screening method for product testing and development. For a better assessment of the cytotoxicity of the vapor phase, a rapid cell-free method has been developed. This paper describes a capillary electrop

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17

Chon, Yang, Lee, et al. "Novel PEEK Copolymer Synthesis and Biosafety – I: Cytotoxicity Evaluation for Clinical Application." Polymers 11, no. 11 (2019): 1803. http://dx.doi.org/10.3390/polym11111803.

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In this research, we synthesized novel polyetheretherketone (PEEK) copolymers and evaluated the biosafety and cytotoxicity of their composites for spinal cage applications in the orthopedic field. The PEEK copolymers and their composites were prepared through a solution polymerization method using diphenyl sulfone as a polymerization solvent. The composite of PEEK copolymer showed good mechanical properties similar to that of natural bone, and also showed good thermal characteristics for the processing of clinical use as spine cage. The results of an in vitro cytotoxicity test did not show any

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18

Räägel, Helin, Audrey Turley, Trevor Fish, et al. "Medical Device Industry Approaches for Addressing Sources of Failing Cytotoxicity Scores." Biomedical Instrumentation & Technology 55, no. 2 (2021): 69–84. http://dx.doi.org/10.2345/0890-8205-55.2.69.

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Abstract To ensure patient safety, medical device manufacturers are required by the Food and Drug Administration and other regulatory bodies to perform biocompatibility evaluations on their devices per standards, such as the AAMI-approved ISO 10993-1:2018 (ANSI/AAMI/ISO 10993-1:2018).However, some of these biological tests (e.g., systemic toxicity studies) have long lead times and are costly, which may hinder the release of new medical devices. In recent years, an alternative method using a risk-based approach for evaluating the toxicity (or biocompatibility) profile of chemicals and materials

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19

Dominici, Luca, Barbara Cerbone, Milena Villarini, Cristina Fatigoni, and Massimo Moretti. "In Vitro Testing for Genotoxicity of Indigo Naturalis Assessed by Micronucleus Test." Natural Product Communications 5, no. 7 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500711.

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In the field of cosmetic dyes, used for coloring the hair and skin, there is a clear tendency to replace the widely used synthetic dyes by natural colorants, such as henna and mixtures of henna with indigo. The aim of this study was to estimate the genotoxicity of water and DMSO solutions of indigo naturalis (prepared from Indigofera tinctoria leaves) using the cytokinesis-blocked micronucleus (CBMN) assay in the human metabolically active HepG2 cell line. The cytotoxic effects of indigo solutions were first assessed by propidium iodide and fluorescein-diacetate simultaneous staining. For both

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20

Kalinin, Roman E., Igor A. Suchkov, Nina D. Mzhavanadze, et al. "Comparison of cytotoxicity of vascular prostheses in vitro." I.P. Pavlov Russian Medical Biological Herald 28, no. 2 (2020): 183–92. http://dx.doi.org/10.23888/pavlovj2020282183-192.

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Aim. To study and compare cytotoxicity of the main types of synthetic prostheses used in arterial reconstructive surgery, including polytetrafluoroethylene (PTFE) and polyethylene-terephthalate (Dacron). Materials and Methods. On the culture of human umbilical vein endothelial cells (HUVEC) of the 3rd passage, MTS test was conducted that is used in laboratory examinations with attraction of cellular technologies to study cytotoxicity of medical drugs and medical products. The test implies use of MTS reagent that is 3-(4,5-dimethylthiazol-2-il)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)

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21

MALCOVATI, M., C. GIORDANI, C. RANZATI, and M. TENCHINI. "A cytotoxicity test based on in vitro cultured human keratinocytes and fibroblasts." Cell Biology International Reports 14 (September 1990): 76. http://dx.doi.org/10.1016/0309-1651(90)90410-z.

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Milhomem-Paixão, Susana Suely Rodrigues, Maria Luiza Fascineli, Luis Alexandre Muehlmann, et al. "Andiroba Oil (Carapa guianensis Aublet) Nanoemulsions: Development and Assessment of Cytotoxicity, Genotoxicity, and Hematotoxicity." Journal of Nanomaterials 2017 (2017): 1–11. http://dx.doi.org/10.1155/2017/4362046.

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Andiroba oil (AO) is obtained from an Amazonian plant and is used in traditional medicine. We carried out a comparative study to test the cytotoxicity, genotoxicity, and hematotoxicity of the oil and its nanoemulsion (AN) in vitro (fibroblasts, lineage NIH/3T3) and in vivo (Swiss mice). The AN was characterized by DLS/Zeta, and its stability was investigated for 120 days. The biological activity of AN was assessed in vitro by MTT test and cell morphology analyses and in vivo by micronucleus, comet, and hematotoxicity tests. The AN presented a hydrodynamic diameter (Hd) of 142.5±3.0 and PDI of

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Hunt, S. M., C. Chrzanowska, C. R. Barnett, and J. K. Fawell. "The Application of In Vitro Cytotoxicity Assays to the Water Industry." Alternatives to Laboratory Animals 14, no. 2 (1986): 64–71. http://dx.doi.org/10.1177/026119298601400203.

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This paper describes areas in which in vitro cytotoxicity assays may be of use to the water industry and reviews the studies that have already been published on water samples and water industry materials. In vitro cytotoxicity assays are useful in comparing aqueous samples and for testing of materials, but direct comparisons of test systems have not been carried out. At present, it is therefore unclear if any one method is particularly appropriate for use with water samples.

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Rodil, S. E., R. Olivares, and H. Arzate. "In vitro cytotoxicity of amorphous carbon films." Bio-Medical Materials and Engineering 15, no. 1-2 (2005): 101–12. https://doi.org/10.1177/0959298920050151-2011.

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Amorphous carbon (a‐C), carbon nitride (a‐CN) and titanium films were deposited on stainless steel substrates (SS) using a dc magnetron sputtering system attached to a high vacuum chamber. Films were deposited using a base pressure of 1.3×10 −4 Pa. For the carbon films a pure graphite target was eroded in an Argon plasma. For the case of the a‐CN films, the Ar flux was substituted by 100% N 2 gas. Titanium films were deposited in a different chamber, using a pure Ti target and an argon plasma. In vitro studies were carried out on the coated samples using human osteoblasts cells. Cytotoxicity o

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de Carlos, Alejandro, Jacinto P. Borrajo, Julia Serra, Pio González, Sara Liste, and Betty León. "In Vitro Cytotoxicity Testing of Wood-Based Biomorphic SiC Ceramics." Key Engineering Materials 284-286 (April 2005): 581–84. http://dx.doi.org/10.4028/www.scientific.net/kem.284-286.581.

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The aim of this study was to test the in vitro cytotoxicity of wood-based biomorphic Silicon Carbide (SiC) ceramics, using MG-63 human osteoblast-like cells. This innovative material has been recently developed and it exhibits unique mechanical properties towards their application in biomedical technology. In the solvent extraction test the SiC ceramic extracts had almost no effect on cellular activity even at 100% concentration. A similar behaviour was found for Ti6Al4V and bioactive glass, used as reference materials. The results of the cell morphology and the cellular attachment response ha

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Kopponen, Päivi, Mirkka Asikainen, Riitta Törrönen, Kaisa Klemola, Jyrki Liesivuori, and Sirpa Kärenlampi. "In Vitro Cytotoxicity of Textile Dyes and Extracts of Dyed/Finished Fabrics." Alternatives to Laboratory Animals 25, no. 5 (1997): 539–46. http://dx.doi.org/10.1177/026119299702500510.

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The aim of this study was to investigate whether an in vitro test can give an indication of the overall toxicity of fabric extracts, and whether this toxicity correlates with the toxicity of the dyes and finishes used. Thirteen textile dyes and dyed/finished cotton fabrics were tested by using the Hepa-1 cytotoxicity test. Black sulphur and two blue reactive dyes were the most toxic, with IC50 (the concentration at which the total protein content was 50% of the protein content of non-exposed cells) values of 40–65μg/ml. The least toxic dyes, the black and yellow mix reactive dyes, had IC50 val

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Zhang, Jian-Qiang, Ke Li, Kun-Ming Jiang, et al. "Development of an oral satraplatin pharmaceutical formulation by encapsulation with cyclodextrin." RSC Advances 6, no. 21 (2016): 17074–82. http://dx.doi.org/10.1039/c5ra27182g.

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A novel water-soluble oral satraplatin/β-cyclodextrin inclusion complex was prepared and characterized with a variety of techniques. The inclusion complex showed much higher antitumor activity in vitro cytotoxicity test and in vivo antitumor test.

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Halle, Willi, Ingo Baeger, Björn Ekwall, and Horst Spielmann. "Correlation between In Vitro Cytotoxicity and Octanol/Water Partition Coefficient of 29 Substances from the MEIC Programme." Alternatives to Laboratory Animals 19, no. 3 (1991): 338–43. http://dx.doi.org/10.1177/026119299101900307.

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Single linear regression analysis was used for chemicals of the MEIC (multicentre evaluation of in vitro cytotoxicity) project, to obtain a correlation between in vitro cytotoxicity determined in various test systems, according to data from the literature, and their lipophilicity. A linear correlation coefficient of 0.76 was determined between the IC50x¯ values (geometrical mean of two or more IC50 values per substance) of 29 MEIC substances and their octanol/water partition coefficient (owp). Since the MEIC substances were selected from a broad spectrum of structurally unrelated classes of ch

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Souza, Klester Santos, Ruth Flavia Vera Villamil Jaimes, Sizue Otta Rogero, Pedro Augusto de Paula Nascente, and Silvia Maria Leite Agostinho. "In Vitro Cytotoxicity Test and Surface Characterization of CoCrW Alloy in Artificial Saliva Solution for Dental Applications." Brazilian Dental Journal 27, no. 2 (2016): 181–86. http://dx.doi.org/10.1590/0103-6440201300513.

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Abstract In order to evaluate its application as a dental prosthesis material, a CoCrW alloy was subjected to in vitro cytotoxicity test, surface characterization and electrochemical studies performed in artificial saliva and 0.15 mol.L-1 NaCl medium. The used techniques were: anodic polarization curves, chronoamperometric measurements, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) analysis and X-ray photoelectron spectroscopy (XPS). Cytotoxicity test was also performed. The electrochemical behavior of CoCrW alloy w

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Clemedson, Cecilia, Elisabeth McFarlane-Abdulla, Marianne Andersson, et al. "MEIC Evaluation of Acute Systemic Toxicity." Alternatives to Laboratory Animals 24, no. 1_part_1 (1996): 251–72. http://dx.doi.org/10.1177/026119299602400102.1.

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The multicentre evaluation of in vitro cytotoxicity (MEIC) study is a programme designed to evaluate the relevance of in vitro toxicity tests for predicting human toxicity, and is organised by the Scandinavian Society for Cell Toxicology. The project started in 1989 and is scheduled to be finished by June 1996. MEIC is a voluntary effort by international laboratories to test the same 50 reference chemicals in their own in vitro toxicity systems. At present, 31 laboratories have submitted results for the first 30 reference chemicals from a total of 68 in vitro cytotoxicity tests. In the definit

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Costa, Isolda, Sizue Ota Rogero, Olandir Vercino Correa, Clarice Terui Kunioshi, and Mitiko Saiki. "Corrosion and Cytotoxicity Evaluation of AISI 316L Stainless Steel Produced by Powder Injection Molding (PIM) Technology." Materials Science Forum 498-499 (November 2005): 86–92. http://dx.doi.org/10.4028/www.scientific.net/msf.498-499.86.

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This study investigates the in vitro corrosion and cytotoxicity response of AISI 316L stainless steel produced by powder injection molding (PIM) technology in a solution that simulates physiological fluids (MEM) by electrochemical techniques and neutral red uptake cytotoxicity assay. The results were compared with those of AISI 316L produced by conventional metallurgy. Both steels showed high corrosion resistance and no toxic effect in the cytotoxicity test. The corrosion products were analyzed by instrumental neutron activation analysis (INAA). The surfaces of the alloys were evaluated before

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Ferreira, Inês, Mafalda Laranjo, Carlos Miguel Marto, et al. "GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study." Molecules 25, no. 18 (2020): 4297. http://dx.doi.org/10.3390/molecules25184297.

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The sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow® bioseal sealer and to compare them with AH26® epoxy resin. Culture media were conditioned with the GuttaFlow® bioseal and AH26® pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types w

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Nagami, Kazuyuki, and Eiji Maki. "In vitro cytotoxicity test for estimating non-ocular irritation dose of ophthalmic solutions." Cell Biology and Toxicology 9, no. 2 (1993): 107–18. http://dx.doi.org/10.1007/bf00757573.

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Vavilov, M. P., A. P. Vorotyntsev, E. A. Luzhnikov, and E. K. Nazarova. "Nonspecific leukocytolysisin vitro as a test of comparative cytotoxicity of low-molecular nonelectrolytes." Bulletin of Experimental Biology and Medicine 119, no. 2 (1995): 165–67. http://dx.doi.org/10.1007/bf02445867.

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Puig-Herreros, Clara, José Luis Sanz, David García-Bernal, et al. "Comparative Cytotoxicity of Menthol and Eucalyptol: An In Vitro Study on Human Gingival Fibroblasts." Pharmaceutics 16, no. 4 (2024): 521. http://dx.doi.org/10.3390/pharmaceutics16040521.

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The aim of this study was to assess the influence of eucalyptol and menthol on the cell viability, migration, and reactive oxygen species production of human gingival fibroblasts (GFs) in vitro. Three different concentrations of eucalyptol and menthol were prepared following ISO 10993-5 guidelines (1, 5, and 10 mM). GFs were isolated from extracted teeth from healthy donors. The following parameters were assessed: cell viability via MTT, Annexin-V-FITC and 7-AAD staining, and IC50 assays; cell migration via horizontal scratch wound assay; and cell oxidative stress via reactive oxygen species a

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Mahmoud, Nouf N., Lubna M. Al-Kharabsheh, Enam A. Khalil, and Rana Abu-Dahab. "Interaction of Gold Nanorods with Human Dermal Fibroblasts: Cytotoxicity, Cellular Uptake, and Wound Healing." Nanomaterials 9, no. 8 (2019): 1131. http://dx.doi.org/10.3390/nano9081131.

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Herein, the cytotoxicity, cellular uptake and wound healing of human dermal fibroblasts were investigated upon treatment with gold nanorods (GNR) decorated with different ligands. Neutral and cationic poly ethylene glycol (PEG)-decorated GNR demonstrated the least cytotoxicity and cellular internalization, while anionic- and bovine serum albumin (BSA)-coated GNR revealed significant cytotoxicity and cellular uptake into human dermal fibroblasts. The cell scratch test demonstrated that neutral, cationic PEGylated GNR and anionic-decorated GNR have accelerated the wound healing rate in vitro aft

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Mačkić-Đurović, Mirela, Amina Aščerić, Dunja Rukavina, and Izeta Aganović-Mušinović. "In vitro analysis of thimerosal genotoxicity and cytotoxicity effect." Asian Journal of Medical Sciences 13, no. 3 (2022): 53–58. http://dx.doi.org/10.3126/ajms.v13i3.41657.

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Background: Thimerosal is an organomercury compound with high mercury content. Thimerosal is located in the market as an effective bacteriostatic in a series of pharmaceutical products, ophthalmic and nasal products, immunoglobulin preparations, and as a preservative in vaccines. Aims and Objectives: Since it is a compound with living content, this study aimed to examine the genotoxic and cytotoxic potential of thimerosal in human lymphocytes culture. Materials and Methods: We used chromosome aberration analysis and cytokinesis-block micronucleus cytome (CBMN-Cyt) assay to test its genotoxic a

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Mirela Mačkić-Đurović, Dzenana Klepo, Amina Aščerić, Dunja Rukavina, and Izeta Aganović-Mušinović. "In vitro analysis of thimerosal genotoxicity and cytotoxicity effect." Asian Journal of Medical Sciences 13, no. 3 (2023): 53–58. https://doi.org/10.71152/ajms.v13i3.3933.

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Background: Thimerosal is an organomercury compound with high mercury content. Thimerosal is located in the market as an effective bacteriostatic in a series of pharmaceutical products, ophthalmic and nasal products, immunoglobulin preparations, and as a preservative in vaccines. Aims and Objectives: Since it is a compound with living content, this study aimed to examine the genotoxic and cytotoxic potential of thimerosal in human lymphocytes culture. Materials and Methods: We used chromosome aberration analysis and cytokinesis-block micronucleus cytome (CBMN-Cyt) assay to test its genotoxic a

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Liu, Hao-Yang, Lei Du, Yan-Teng Zhao, and Wei-Qun Tian. "In Vitro Hemocompatibility and Cytotoxicity Evaluation of Halloysite Nanotubes for Biomedical Application." Journal of Nanomaterials 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/685323.

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Halloysite nanotubes (HNTs), due to their unique structures and properties, may play an important role in biomedical applications. In vitro test is usually conducted as a preliminary screening evaluation of the hemocompatibility and cytotoxicity of HNTs for its short term consuming, convenience, and less expense. In this work, HNTs were processed with anticoagulated rabbit blood to detect its blood compatibility. The result of hemolysis test shows that the hemolysis ratios are below 0.5%, indicating nonhemolysis of HNTs. Plasma recalcification time suggests that HNTs are dose-dependently contr

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Riddell, R. J., D. S. Panacer, S. M. Wilde, R. H. Clothier, and M. Balls. "The Importance of Exposure Period and Cell Type in In Vitro Cytotoxicity Tests." Alternatives to Laboratory Animals 14, no. 2 (1986): 86–92. http://dx.doi.org/10.1177/026119298601400205.

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The cytotoxicities of 50 coded chemicals from the FRAME blind trial set were estimated by the FRAME kenacid blue (KB) and Rockefeller neutral red (NR) assay methods after test periods of 24 hours and 72 hours with murine 3T3 fibroblasts. Results obtained after the two test periods were considered to be similar for 25 of the chemicals, but different for 25 others. Our results indicate that a test period of 72 hours is more suitable for the assessment of potential cytotoxicity by the KB and NR methods than the 24 hour test period, which is less likely to allow sufficient time for certain chemica

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Hussein, Rajaa A. "Actinomycin- Production Assay: A simple Bacteriological Method For Antitumor Activity Screening In Vitro." Kufa Journal For Veterinary Medical Sciences 2, no. 2 (2011): 116–26. http://dx.doi.org/10.36326/kjvs/2011/v2i23909.

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We present and evaluate the actinomycin-production assay, a bacterial test for detecting DNA-damaging agents as a primary step for detecting the antitumor activity of new synthesized, modified or isolated compounds. It’s a simple test based on the activity of the agents against actinomycin antibiotic production of Streptomyces antibioticus, bacteria whose value is monitored by the inhibition activity of E. coli and S. aureus bacterial growth using two standard anticancer drugs cisplatin and flurouracil. In this assay, production of actinomycin antibiotic is measured as a specific indication of

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Hussein, Rajaa A. "Actinomycin- Production Assay: A simple Bacteriological Method For Antitumor Activity Screening In Vitro." Kufa Journal For Veterinary Medical Sciences 2, no. 2 (2011): 116–26. http://dx.doi.org/10.36326/kjvs/2011/v2i23909.

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We present and evaluate the actinomycin-production assay, a bacterial test for detecting DNA-damaging agents as a primary step for detecting the antitumor activity of new synthesized, modified or isolated compounds. It’s a simple test based on the activity of the agents against actinomycin antibiotic production of Streptomyces antibioticus, bacteria whose value is monitored by the inhibition activity of E. coli and S. aureus bacterial growth using two standard anticancer drugs cisplatin and flurouracil. In this assay, production of actinomycin antibiotic is measured as a specific indication of

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Nielsen, Hans Ingolf, and Kjell Bertheussen. "The Medi-Cult® Hybritest for In Vitro Toxicology and Quality Control." Alternatives to Laboratory Animals 17, no. 3 (1990): 199–202. http://dx.doi.org/10.1177/026119299001700313.

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The Medi-Cult® Hybritest is presented as a new, general test for in vitro toxicology and routine quality control for cytotoxicity in a variety of products. The test is based upon the growth rate of a sensitive, anchorage-independent cell line, which proliferates rapidly in a protein-free medium, thereby avoiding neutralisation of toxins by serum proteins.

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Pustovoyt, V. I., T. A. Astrelina, E. I. Balakin, et al. "Evaluation of in vitro cytotoxicity of «ACVR-IN-01» in a human mesenchymal stromal cell model." Infekcionnye bolezni 20, no. 3 (2022): 83–91. http://dx.doi.org/10.20953/1729-9225-2022-3-83-91.

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Objective. Evaluate the cytotoxicity of a new chemical compound «ACVR-IN-01» (in vitro) on a culture of human mesenchymal stromal cells (MSCs). Materials and methods. The characterized MSC culture of the 4th passage from the biobank of cell cultures was used. Test samples «ACVR-IN-01» were prepared at concentrations of 25, 200 and 400 μg/ml. For each tested concentration of «ACVRIN-01» cultivation was carried out in triplicate. A visual assessment of the state of the cell culture was performed after 24, 48 hours and 7 days. The assessment of the functional state of MSCs was carried out by iden

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Harti, Shreya A., M. S. Adarsha, N. Meena, N. S. Priya, L. Vijayalakshmi, and Akshata J. Airsang. "An in vitro assessment of cytotoxicity and genotoxicity of root repair materials." Journal of Oral and Maxillofacial Pathology 27, no. 4 (2023): 700–705. http://dx.doi.org/10.4103/jomfp.jomfp_467_23.

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Background: The success of the root-end procedure depends on the regeneration of the functional periodontal attachment system, including the cementum on the resected root-end surface, periodontal ligament (PDL), and alveolar bone. As root end filling materials remain in close contact with live periapical tissues, they may influence the endodontic treatment outcome. Aim: To assess and compare the cytotoxicity and genotoxicity of three root repair materials, mineral trioxide aggregate (MTA), endosequence, and geristore in human-cultured periodontal ligament fibroblasts. Materials and Methods: Cu

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Martina, Stefano, Roberto Rongo, Rosaria Bucci, Armando Viviano Razionale, Rosa Valletta, and Vincenzo D'Antò. "In vitro cytotoxicity of different thermoplastic materials for clear aligners." Angle Orthodontist 89, no. 6 (2019): 942–45. http://dx.doi.org/10.2319/091718-674.1.

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ABSTRACT Objectives: To investigate the in vitro cytotoxicity of different thermoplastic materials for clear aligners on human primary gingival fibroblasts (HGFs). Materials and Methods: Four materials for clear aligners were considered in this study: Duran (Scheu-Dental GmbH, Iserlohn, Germany), Biolon (Dreve Dentamid GmbH, Unna, Germany), Zendura (Bay Materials LLC, Fremont, CA, USA), and SmartTrack (Align Technology, San Jose, CA, USA). Three out of four materials (Duran, Biolon, Zendura) were assessed as thermoformed and nonthermoformed, whereas the SmartTrack was assessed only as thermofo

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Rosli, Nurfarhana, Sandra Catherine Zainathan, and Siti Nor Khadijah Addis. "Biosynthesis and Cytotoxic Activity of In Vitro Expressed Scygonadin Protein." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 17, no. 3 (2022): 141–45. http://dx.doi.org/10.15578/squalen.699.

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Antimicrobial peptides (AMP) are key components of an innate immune response which represent immediate action of the defence mechanism of an organism. It is considered a novel therapeutic agent due to its abundance in nature and a broad range of defence activity against microbial. Preceding research has shown that scygonadin AMPs isolated from seminal plasma of mud crab had the potential as a novel antimicrobial agent. However, its cytotoxicity properties on cultured cells have never been experimentally addressed. In this study, the scygonadin protein was expressed in vitro, followed by cytoto

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Ja’apar, Siti Aisyah Nadirah, Solachuddin Jauhari Arief Ichwan, and Musliana Mustaffa. "In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells." Archives of Orofacial Sciences 17, no. 2 (2022): 209–24. http://dx.doi.org/10.21315/aos2022.1702.oa05.

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This study evaluated the cytotoxicity of four bioceramic root canal sealers (bioceramic sealers): GuttaFlow Bioseal (GB), MTA Fillapex, CeraSeal Bioceramic root canal sealer (CS), and iRoot SP root canal sealer (iRSP). The viability of human gingival fibroblast (HGF) cells was used to evaluate the cytotoxicity of these bioceramic sealers. HGF cells were cultured and exposed to bioceramic sealer extracts for 24 hours, 48 hours and 72 hours at 37°C in an incubator humidified with 5% CO2. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide or MTT assay was conducted to determine ce

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Babich, Harvey, and Ellen Borenfreund. "Applications of the Neutral Red Cytotoxicity Assay to In Vitro Toxicology." Alternatives to Laboratory Animals 18, no. 1_part_1 (1990): 129–44. http://dx.doi.org/10.1177/026119299001800116.1.

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A concerted effort is currently in progress to develop alternatives to the use of live animals for the acute toxicity testing of xenobiotics. To this end, the neutral red in vitro cytotoxicity assay was developed which, although initially based on the use of mammalian cells in culture, has also been adapted for ecotoxicity studies using fish cells in culture. The neutral red assay is based on the binding of neutral red, a weakly cationic supravital dye, to the lysosomal matrix of viable cells after their incubation with toxic agents. Spectrophotometric quantitation of the extracted dye with a

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Missier, Mary Sheloni, R. Mahesh, S. P. Saravana Dinesh, S. Rajeshkumar, and V. Amalorpavam. "In-Vitro Cytotoxic Evaluation of Titanium Dioxide Nanoparticle Using L929 Cell Lines." Journal of Pharmacy and Bioallied Sciences 16, Suppl 2 (2024): S1468—S1473. http://dx.doi.org/10.4103/jpbs.jpbs_824_23.

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ABSTRACT Tooth movement in orthodontic therapy is connected to the frictional force that is created as the wire passes over the bracket. It is possible for teeth to move quickly when friction is at a minimum. Wires coated with nanoparticles have been found to reduce friction as a result of current developments in the orthodontics sector. Having an antimicrobial property is an added benefit. To reduce the friction generated by brackets, wires, and bands by lessening their cytotoxicity, this study examines the potential use of TiO2 nanoparticles in orthodontics. A monolayer of L929 was utilized

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