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Artykuły w czasopismach na temat „Lipopolysaccharide antigens”

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Data publikacji: 3 czerwca 2025

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1

Smith, Bradford P., George W. Dilling, John K. House, Hans Konrad, and Nadia Moore. "Enzyme-Linked Immunosorbent Assay for Salmonella Serology using Lipopolysaccharide Antigen." Journal of Veterinary Diagnostic Investigation 7, no. 4 (1995): 481–87. http://dx.doi.org/10.1177/104063879500700410.

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Enzyme-linked immunosorbent assay (ELISA) using Salmonella lipopolysaccharide (LPS) to measure specific IgG titers in cattle has proven useful. Serology can be used to assess vaccine responses and infection rates, to detect carriers, and to aid in epidemiologic studies. The objective of this study was to assess cross-reactions using sera from cattle vaccinated with different Salmonella serogroups. ELISA plates using lipopolysaccharide from serogroup B, C1, C3, D1 or E1 as the plate antigens were tested. LPS was extracted from Salmonella typhimurium (Serogroup B; somatic antigens 01, 4, 12), S.

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2

Mutharia, Lucy W., Bonnie T. Raymond, Teri R. Dekievit, and Roselynn M. W. Stevenson. "Antibody specificities of polyclonal rabbit and rainbow trout antisera against Vibrio ordalii and serotype 0:2 strains of Vibrio anguillarum." Canadian Journal of Microbiology 39, no. 5 (1993): 492–99. http://dx.doi.org/10.1139/m93-070.

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Polyclonal rabbit antisera raised against Vibrio ordalii and serotype 02 strains of Vibrio anguillarum showed extensive cross-reactivity with lipopolysaccharide from these bacterial pathogens of fish when tested in western immunoblot analysis. Results with absorbed polyclonal antisera indicated that lipopolysaccharide molecules from these strains had both common and strain-specific antigenic determinants, which allowed the antisera to be used to differentiate between V. ordalii and serotype 02 strains of V. anguillarum. Unlike rabbits, the immune response in rainbow trout to serotype 02 common

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3

Rani, Mamta, Rajesh K. Gupta, and S. Chhibber. "Protection against Klebsiella pneumoniae induced lobar pneumonia in rats with lipopolysaccharide and related antigens." Canadian Journal of Microbiology 36, no. 12 (1990): 885–90. http://dx.doi.org/10.1139/m90-153.

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The immunoprotective role of lipopolysaccharide and related antigens from Klebsiella pneumoniae was studied in a lobar pneumonia model developed in rats. Various antigens were obtained by different chemical treatments of the lipopolysaccharide. All these antigens (purified lipopolysaccharide, reduced lipopolysaccharide, lipopolysaccharide – bovine serum albumin complex, and lipid A – bovine serum albumin complex were tested for pyrogenicity and the Shwartzman reaction. The lipopolysaccharide and the various related antigens were pyrogenic and elicited a positive Shwartzman reaction at high con

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4

Maclean, Ian W., Rosanna W. Peeling, and Robert C. Brunham. "Characterization of Chlamydia trachomatis antigens with monoclonal and polyclonal antibodies." Canadian Journal of Microbiology 34, no. 2 (1988): 141–47. http://dx.doi.org/10.1139/m88-028.

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We used monoclonal antibodies (MAbs) to examine the antigenic specificity and biologic function of several Chlamydia trachomatis antigens. Thirteen distinct MAbs to eight C. trachomatis antigens were produced. Six MAbs reacted with unique epitopes on the major outer membrane protein (MOMP) and two of these had neutralizing activity. MAbs were produced to each of the chlamydial antigens with molecular masses of 10, 29, 32, 57, 60, 70, and 75 kilodaltons (kDa). These MAbs showed species and genus specificity in an immunoblot assay. None of the MAbs had neutralizing activity. The epitopes recogni

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5

Whitfield, C. "Biosynthesis of lipopolysaccharide O antigens." Trends in Microbiology 3, no. 5 (1995): 178–85. http://dx.doi.org/10.1016/s0966-842x(00)88917-9.

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6

Grossman, N., K. A. Joiner, M. M. Frank, and L. Leive. "C3b binding, but not its breakdown, is affected by the structure of the O-antigen polysaccharide in lipopolysaccharide from Salmonellae." Journal of Immunology 136, no. 6 (1986): 2208–15. http://dx.doi.org/10.4049/jimmunol.136.6.2208.

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Abstract Bacteria whose lipopolysaccharide contains O-antigen side chains activate complement via the alternative pathway. We have shown previously that three strains of Salmonella, differing in the chemical structure of their O-antigens, consumed C3 to different extents when incubated in C4-deficient guinea pig serum. Moreover, sheep erythrocytes coated with lipopolysaccharide purified from these strains mimicked whole cells in C3 consumption, proving that lipopolysaccharide alone could account for these results. We have now measured the deposition of 125I-C3 in this system, and found that C3

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7

Whitfield, Chris, Danielle M. Williams, and Steven D. Kelly. "Lipopolysaccharide O-antigens—bacterial glycans made to measure." Journal of Biological Chemistry 295, no. 31 (2020): 10593–609. http://dx.doi.org/10.1074/jbc.rev120.009402.

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Lipopolysaccharides are critical components of bacterial outer membranes. The more conserved lipid A part of the lipopolysaccharide molecule is a major element in the permeability barrier imposed by the outer membrane and offers a pathogen-associated molecular pattern recognized by innate immune systems. In contrast, the long-chain O-antigen polysaccharide (O-PS) shows remarkable structural diversity and fulfills a range of functions, depending on bacterial lifestyles. O-PS production is vital for the success of clinically important Gram-negative pathogens. The biological properties and functi

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8

Paci, Valentina, Pierina Visciano, Ivanka Krasteva, et al. "Identification of Immunogenic Candidate for New Serological Tests for Brucella melitensis by a Proteomic Approach." Open Microbiology Journal 15, no. 1 (2021): 92–97. http://dx.doi.org/10.2174/1874285802115010092.

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Background: The diagnosis of brucellosis by serological tests is based on antigen suspensions derived from smooth lipopolysaccharide extracts, which can give false positive results linked to cross-reactivity with other Gram-negative microorganisms, especially Yersinia enterocolitica O:9 and Escherichia coli O157:H7. Objective: The objective of the present study was the characterization by proteomic analysis of specific immunogenic proteins not associated with smooth lipopolysaccharide to improve the diagnostic tests used in the ovine brucellosis eradication programs. Methods: The serum from a

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9

Marsden, Brian J., David R. Bundle, and Malcolm B. Perry. "Serological and structural relationships between Escherichia coli O: 98 and Yersinia enterocolitica O: 11,23 and O: 11,24 lipopolysaccharide O-antigens." Biochemistry and Cell Biology 72, no. 5-6 (1994): 163–68. http://dx.doi.org/10.1139/o94-024.

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The serologically related lipopolysaccharide O-antigens of Yersinia enterocolitica serotypes 11,23 and 11,24 and of Escherichia coli O: 98 were analysed by composition analysis, methylation, and the use of one- and two-dimensional nuclear magnetic reasonance methods. They were found to be composed of the same basic linear unbranched polysaccharide of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 2-acetamido-2-deoxy-D-galacturonic acid (D-GalNAcA), and 2-acetamido-2,6-dideoxy-L-glucose (L-quinovosamine, L-QuiNAc), having the structure[Formula: see text]The

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10

Lozniewski, Alain, Xavier Haristoy, David A. Rasko, et al. "Influence of Lewis Antigen Expression by Helicobacter pylori on Bacterial Internalization by Gastric Epithelial Cells." Infection and Immunity 71, no. 5 (2003): 2902–6. http://dx.doi.org/10.1128/iai.71.5.2902-2906.2003.

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ABSTRACT The role of Helicobacter pylori lipopolysaccharide (LPS) Lewis antigens in infection is still not well known. We investigated the influence of Lewis antigen expression by H. pylori on its internalization by AGS cells and the epithelium of human gastric xenografts in nude mice using isogenic mutants in LPS biosynthetic genes. In vivo, colonization rates were unaffected by the change in H. pylori Lewis antigen expression, whereas the number of viable intracellular bacteria was significantly higher with wild-type H. pylori strains expressing Lewis antigens when compared to the isogenic m

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11

Hampson, D. J., J. R. L. Mhoma, and B. Combs. "Analysis of lipopolysaccharide antigens ofTreponema hyodysenteriae." Epidemiology and Infection 103, no. 2 (1989): 275–84. http://dx.doi.org/10.1017/s0950268800030636.

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SUMMARYLipopolysaccharide (LPS) extracts obtained fromTreponema hyodysenteriaeof serogroups A, B, D and E, and fromT. innocenswere examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit sera. All organisms possessed multiple LPS bands, but their position and number differed.Immunoblotting of LPS with grouping sera identified three or four major antigenic LPS components in the 10-42 kDa range in all organisms: these components were largely specific to each type-organism of a serogroup, and presumably represented group antigens.

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12

Penaredondo, Maria V., Samuel K. Maheswaran, Laurence O. Whiteley, Trevor R. Ames, and Joel R. Leininger. "Production and partial characterization of monoclonal antibodies to Pasteurella haemolytica A1 capsular polysaccharide and lipopolysaccharide." American Journal of Veterinary Research 49, no. 11 (1988): 1962–68. https://doi.org/10.2460/ajvr.1988.49.11.1962.

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SUMMARY Hybridoma-derived monoclonal antibodies (mab) against the cell surface antigens of Pasteurella haemolytica serotype 1 were obtained by the fusion of murine myeloma cells (P3 × 63 − Ag 8.653) with splenocytes of BALB/c mice immunized with crude logarithmic growth-phase culture supernatant. Initial screening was performed, using an elisa, with the same bacterial growth culture supernatant as coating antigens. Further selection was done, using a panel of purified antigens—either capsular polysaccharide or lipopolysaccharide—as the coating antigen in an elisa, and then performing a leukoto

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13

Li, Hong, Hong Tang, Aleksandra Debowski, Keith Stubbs, Barry Marshall, and Mohammed Benghezal. "Lipopolysaccharide Structural Differences between Western and Asian Helicobacter pylori Strains." Toxins 10, no. 9 (2018): 364. http://dx.doi.org/10.3390/toxins10090364.

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Recent structural analysis of the lipopolysaccharide (LPS) isolated from Helicobacter pylori G27 wild-type and O-antigen ligase mutant resulted in the redefinition of the core-oligosaccharide and O-antigen domains. The short core-oligosaccharide (Glc–Gal–Hep-III–Hep-II–Hep-I–KDO) and its attached trisaccharide (Trio, GlcNAc–Fuc–Hep) appear to be highly conserved structures among H. pylori strains. The G27 LPS contains a linear glucan–heptan linker between the core-Trio and distal Lewis antigens. This linker domain was commonly identified in Western strains. In contrast, out of 12 partial LPS s

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14

RAOUF MAHDI, NITHAL. "PRODUCTION AND CHARACTERIZATION OF THREE BRUCELLA ANTIGENS, LIPOPOL- YSACCHRIDE (LPS), SONICATED CELLS AND WHOLE CELLS ANTIGEN." Iraqi Journal of Veterinary Medicine 20, no. 1 (1996): 13–23. http://dx.doi.org/10.30539/ijvm.v20i1.1569.

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Three types of Brucella abortus antigens were prepared: Lipopolysaccharide (LPS), sonicated cells and whole cells killed antigens. Enzyme-linked immunosorbent assay (ELSIA) was carried out in polysterene microtiter plates using horse-radish peroxidase conjugated to anti- normal bovine serum globuline with hydrogen peroxide and ortho-phenylenediamine as hydrogen as substrate. The results showed that the whole Brucella cells antigen gave the best distinguish between the positive and negative sera with lowest cross reactive with E. coli antiserum

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15

Pantophlet, Ralph, Lore Brade, Lenie Dijkshoorn, and Helmut Brade. "Specificity of Rabbit Antisera against Lipopolysaccharide of Acinetobacter." Journal of Clinical Microbiology 36, no. 5 (1998): 1245–50. http://dx.doi.org/10.1128/jcm.36.5.1245-1250.1998.

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Acinetobacter has been reported to be involved in hospital-acquired infections with increasing frequency. However, clinical laboratories still lack simple methods that allow the accurate identification of Acinetobacter strains at the species level. For this study, proteinase K-digested whole-cell lysates from 44 clinical and environmental isolates were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with hyperimmune rabbit sera to examine the possibility of developing a serotyping scheme based on the O antigen of Acinetobacterlipopolysaccharide (LPS

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16

Tsuji, M., and M. Matsumoto. "Immunochemical relationship of three antigens purified from Pasteurella multocida strain P-1059." American Journal of Veterinary Research 49, no. 9 (1988): 1510–15. https://doi.org/10.2460/ajvr.1988.49.09.1510.

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SUMMARY Three antigens were prepared from a type-3 avian strain of Pasteurella multocida, and their chemical and immunologic characteristics were studied. An antigen, designated 2.5S, was extracted with 2.5% NaCl solution and purified by chromatography. Lipopolysaccharide (lps) was extracted with phenol-water, and a third antigen, designated fs, was extracted in 0.3% formalin solution containing 0.85% NaCl and purified by differential centrifugation. The 2.5S and the fs antigens consisted of 40% protein and 15% carbohydrate, whereas lps did not contain a substantial amount of protein. A major

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17

Chernusky, V. G., N. N. Popov, O. L. Govalenkova, A. V. Letyago, V. L. Kashina-Yarmak, and T. V. Evdokimovа. "ANTIGENS OF THE BRONCHOPULMONARY SYSTEM AND THEIR ROLE IN THE DIAGNOSIS OF AUTOIMMUNE PROCESS IN CHILDREN WITH BRONCHIAL ASTHMA." Annals of Mechnikov Institute, no. 2 (June 7, 2017): 15–19. https://doi.org/10.5281/zenodo.803846.

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<strong>Introduction.</strong> One of the promising directions of improvement of immunodiagnostic BA recently recognized the creation of tissue antigens of the respiratory system structures and their use in diagnostic assays to detect subtle disturbances in the structure of the respiratory system in the development of this disease. Each of the body tissue has an extremely wide range of proteins that characterize its species and organ specificity of the individual. It follows that satisfactory diagnostic results can be obtained only if the isolation of individual proteins characteristic lesion

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18

Preston, Martin A., and J. L. Penner. "Characterization of cross-reacting serotypes of Campylobacter jejuni." Canadian Journal of Microbiology 35, no. 2 (1989): 265–73. http://dx.doi.org/10.1139/m89-040.

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Some strains of Campylobacter jejuni react with more than one reference antiserum from the serotyping scheme based on heat-stable lipopolysaccharide antigens. To investigate the molecular basis of these cross-reactions, lipopolysaccharides from the reference strains for serotypes 4, 13, 16, 43, and 50 and isolates recovered during two different outbreaks of C. jejuni enteritis were analyzed by passive haemagglutination and sodium dodecyl sulphate – polyacrylamide gel electrophoresis coupled with silver staining or immunoblotting. The results showed that lipopolysaccharides from the reference s

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19

Pitt, T. L., Hazel Aucken, and D. A. B. Dance. "Homogeneity of lipopolysaccharide antigens in Pseudomonas pseudomallei." Journal of Infection 25, no. 2 (1992): 139–46. http://dx.doi.org/10.1016/0163-4453(92)93920-l.

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20

Mosier, Derek A., K. René Simons, and Jerome G. Vestweber. "Passive protection of calves with Pasteurella haemolytica antiserum." American Journal of Veterinary Research 56, no. 10 (1995): 1317–21. http://dx.doi.org/10.2460/ajvr.1995.56.10.1317.

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SUMMARY Four colostrum-deprived calves each were immunized passively with antisera to whole Pasteurella haemolytica, leukotoxin-containing supernatants of P haemolytica, P haemolytica lipopolysaccharide, or newborn calf serum. Calves were challenge exposed intrabronchially with 5 × 109 P haemolytica, and 24 hours later, the resulting lesions were evaluated. The greatest protection against challenge exposure was provided by the antiserum to whole P haemolytica (lesion score = 6.3), whereas newborn calf serum provided the least protection (lesion score = 28.3). Calves that received antiserum to

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21

Duong, Bao Hoa, Hua Tian, Takayuki Ota, et al. "Decoration of T-independent antigen with ligands for CD22 and Siglec-G can suppress immunity and induce B cell tolerance in vivo." Journal of Experimental Medicine 207, no. 1 (2009): 173–87. http://dx.doi.org/10.1084/jem.20091873.

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Autoreactive B lymphocytes first encountering self-antigens in peripheral tissues are normally regulated by induction of anergy or apoptosis. According to the “two-signal” model, antigen recognition alone should render B cells tolerant unless T cell help or inflammatory signals such as lipopolysaccharide are provided. However, no such signals seem necessary for responses to T-independent type 2 (TI-2) antigens, which are multimeric antigens lacking T cell epitopes and Toll-like receptor ligands. How then do mature B cells avoid making a TI-2–like response to multimeric self-antigens? We presen

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22

Tyler, Jeff W., James S. Cullor, Mark C. Thurmond, David W. Hird, and Kent M. Parker. "Humoral recognition of lipopolysaccharide core antigens of gram-negative bacteria in neonatal swine." American Journal of Veterinary Research 50, no. 1 (1989): 126–30. https://doi.org/10.2460/ajvr.1989.50.01.126.

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SUMMARY Serologic recognition of common lipopolysaccharide core antigens has been related to enhanced resistance to gramnegative bacterial disease in several species. Class-specific titers (IgG, IgM) were determined by direct ELISA, using intact Escherichia coli (J5) as a plate antigen. Serum samples were obtained from 224 neonatal swine between the ages of 36 and 60 hours. The mean (± SEM) log10 IgG titer against gram-negative core antigens was 1:1,713 ± 0.4718 and the mean log10 IgM titer was 1:202 ± 0.5644. The IgG titer was directly related with litter size, birth weight, and serum total I

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23

Tsuji, M., and M. Matsumoto. "Evaluation of relationship among three purified antigens from Pasteurella multocida strain P-1059 and of their protective capacities in turkeys." American Journal of Veterinary Research 49, no. 9 (1988): 1516–21. https://doi.org/10.2460/ajvr.1988.49.09.1516.

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SUMMARY Three antigens were prepared from Pasteurella multocida strain P-1059, and their immunogenicity and antigenic relationships were investigated. The 3 antigens were a soluble antigen purified from a 2.5% NaCl extract (2.5S), a similar antigen purified from an extract in 0.3% formalin solution containing 0.85% NaCl (fs), and lipopolysaccharide (lps). The antigens were treated with various chemicals and enzymes to study their antigenic and immunogenic determinants. Antigenic analyses with elisa inhibition tests indicated that 2.5S and fs were similar lps-protein complex antigens. The 2.5S

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24

Lee, S. K., and R. T. Woodland. "Selective effect of irradiation on responses to thymus-independent antigen." Journal of Immunology 134, no. 2 (1985): 761–64. http://dx.doi.org/10.4049/jimmunol.134.2.761.

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Abstract Low doses of ionizing radiation have a selective immunosuppressive effect on in vivo B cell responses to thymus-independent (TI) antigens. The B cell response, assayed as direct anti-trinitrophenyl (TNP)-specific plaque-forming cells (PFC), induced by type 2, TI antigens (TNP-Ficoll or TNP-Dextran), was reduced, on the average, by 10-fold in animals exposed to 200 rad of ionizing radiation 24 hr before antigen challenge. In contrast, PFC responses to type 1, TI antigens (TNP-lipopolysaccharide or TNP-Brucella abortus) are unaffected in mice exposed to the same dose of radiation. Adopt

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25

Pacinelli, Elvia, Lei Wang, and Peter R. Reeves. "Relationship of Yersinia pseudotuberculosis O Antigens IA, IIA, and IVB: the IIA Gene Cluster Was Derived from That of IVB." Infection and Immunity 70, no. 6 (2002): 3271–76. http://dx.doi.org/10.1128/iai.70.6.3271-3276.2002.

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ABSTRACT O antigen is part of the lipopolysaccharide present in the outer membrane of gram-negative bacteria and is highly polymorphic. In this study, we obtained sequences of the O-antigen gene clusters for the Yersinia pseudotuberculosis antigens IA, IIA, and IVB. We propose that the IIA gene cluster was derived from the IVB cluster, one of the very few cases in which a parent gene cluster is identified, and that the IA gene cluster could be a hybrid of the IVB and IB gene clusters. All three O antigens contain 6-deoxy-d-mannoheptose, and we identified six genes for the biosynthetic pathway

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26

Chernusky, V. G., M. M. Popov, G. V. Letyago, O. L. Govalenkova, S. R. Tolmacheva, and A. M. Popova. "Role of autoimmune disorders in the pathogenesis of cerebrovascular insufficiency in children with bronchial asthma." PROBLEMS OF UNINTERRUPTED MEDICAL TRAINING AND SCIENCE 41, no. 1 (2021): 37–43. http://dx.doi.org/10.31071/promedosvity2021.01.037.

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It has been shown that the main spectrum of immunopathological reactions in bronchial asthma in children has a clear antigenic dependence not only on the inflammatory-activated intermediate stroma of the bronchopulmonary system, but also on the effects of autoantibodies on cerebral vessels and cell tissue. insufficiency of this contingent of children, which is currently insufficiently studied. The aim is to study autoimmune disorders in the pathogenesis of cerebrovascular insufficiency in children with bronchial asthma. 121 patients with asthma aged 5 to 15 years in the period of exacerbation

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27

Slutzky, Gerald M., Mauricio V. Londner, and Charles L. Greenblatt. "Lipid and Lipopolysaccharide-Like Antigens of Leishmania Promastigotes1." Journal of Protozoology 32, no. 2 (1985): 347–52. http://dx.doi.org/10.1111/j.1550-7408.1985.tb03064.x.

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Yokota, Shin-ichi, Ken-ichi Amano, Shunji Hayashi, Toru Kubota, Nobuhiro Fujii, and Takashi Yokochi. "Human Antibody Response to Helicobacter pylori Lipopolysaccharide: Presence of an Immunodominant Epitope in the Polysaccharide Chain of Lipopolysaccharide." Infection and Immunity 66, no. 6 (1998): 3006–11. http://dx.doi.org/10.1128/iai.66.6.3006-3011.1998.

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ABSTRACT We have examined the antibody response to Helicobacter pylori lipopolysaccharides (LPS) in humans. We used sera from patients with gastroduodenal diseases and healthy adults infected or not infected with H. pylori. Data from the experiments for antibody binding to LPS suggested that the polysaccharide chains from many H. pylori strains showed high immunogenicity in humans. Sera from most (above 70%) H. pylori-infected individuals contained immunoglobulin G (IgG) antibodies against the polysaccharide region highly immunogenic H. pylori LPS. The IgG titers of individual serum samples th

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29

Nelson, Michelle, Joann L. Prior, M. Stephen Lever, Helen E. Jones, Timothy P. Atkins, and Richard W. Titball. "Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis." Journal of Medical Microbiology 53, no. 12 (2004): 1177–82. http://dx.doi.org/10.1099/jmm.0.45766-0.

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Burkholderia pseudomallei is the causative agent of melioidosis, which is a major cause of morbidity and mortality in endemic regions. Currently there is no human vaccine against melioidosis. In this study, LPS or capsular polysaccharide was used to immunize BALB/c mice. The different polysaccharide antigens induced antibody responses. Mice vaccinated with LPS developed predominantly IgM and IgG3 responses. Contrastingly, mice vaccinated with capsular polysaccharide developed a predominantly IgG2b response. After immunization, mice were challenged by the intra-peritoneal route and an increased

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30

M .Mhanam‎, Hatem, Ather K. Kassab‎, and Esmail K. Shubber‎. "Isolation and identification of the causative agent of fowl cholera in ‎Iraq and preparation of vaccines against the disease ‎2- Preparation of different vaccines against fowl cholera disease." Iraqi Journal of Veterinary Medicine 29, no. 1 (2005): 22–32. https://doi.org/10.30539/ztkch564.

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Three types of locally isolated Pasteurella multocida antigenes namely the whole sonicated bacteria (WSB) the bacterial cell wall (BCW) and the lipopolysaccharide (LPS) of bacteria were prepared as a vaccine . Each type of antigenes was used in 3 concentrations that are 107, 109 and 1011 Colony Forming Units / bird for WSB and 250,500 and 750 mg/bird for BCW and LPS. Each of the prepared concentrations was injected subcutaneously into a group of broiler breeder chickens and one group was left as a control . The groups were vaccinated 2 times at 10 and 18 weeks. The progress of immune response

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31

McVey, D. Scott, and Raymond W. Loan. "Total and antigen-specific serum immunoglobulin isotype concentrations in hyperimmunized cattle that have undergone plasmapheresis." American Journal of Veterinary Research 50, no. 5 (1989): 758–61. https://doi.org/10.2460/ajvr.1989.50.05.758.

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SUMMARY The effects of prolonged plasmapheresis of cattle on total and antigen-specific immunoglobulin production were evaluated. Five adult cows were hyperimmunized by repeated iv administration of live, logarithmic-phase Pasteurella haemolytica A1 organisms. Three of the cows underwent plasmapheresis daily for 3 weeks. From 2 cows, serum was only obtained periodically. Anti-P haemolytica antibody was assayed by indirect hemagglutination and a kinetic-augmented, antigen-capture elisa for capsular polysaccharide and lipopolysaccharide/outer membrane protein antigens. Total serum immunoglobulin

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32

Taylor, H. R., S. L. Johnson, J. Schachter, H. D. Caldwell, and R. A. Prendergast. "Pathogenesis of trachoma: the stimulus for inflammation." Journal of Immunology 138, no. 9 (1987): 3023–27. http://dx.doi.org/10.4049/jimmunol.138.9.3023.

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Abstract Active trachoma is characterized by chronic inflammation of the conjunctiva, and repeated episodes of reinfection are thought to be necessary to sustain this inflammation. It is currently believed that much of the tissue damage is immunologically mediated. To identify which antigens might be responsible for stimulating this continued inflammation, cynomolgus monkeys that had recovered from a previous ocular infection with Chlamydia trachomatis were challenged with various antigen preparations. Purified preparations of formalin- or UV-inactivated elementary bodies did not elicit any in

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Yu, Wenhao, Xuhong Yuan, Peng Zhai, Xiaoyun Li, and Caixia Han. "Effects of Trichinella spiralis excretory-secretory antigens on expression of indoleamine 2, 3-dioxygenase on dendritic cells in vitro." Parasite 32 (2025): 26. https://doi.org/10.1051/parasite/2025018.

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Indoleamine 2, 3-dioxygenase (IDO) is a potent immunoenzyme found in dendritic cells (DCs). Research has demonstrated that Trichinella spiralis induces IDO expression in the host immune response through its excretory-secretory (ES) antigens. However, the role of IDO in the immune response to T. spiralis remains unclear. To examine the effects of T. spiralis ES antigens on IDO expression in DCs in vitro, assessments were conducted using qRT-PCR, Western blotting (WB), flow cytometry, and siRNA transfer. The findings indicated that ES antigen stimulation upregulated IDO expression in DCs in vitr

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Kamei, Akinobu, Yamara S. Coutinho-Sledge, Joanna B. Goldberg, Gregory P. Priebe, and Gerald B. Pier. "Mucosal Vaccination with a Multivalent, Live-Attenuated Vaccine Induces Multifactorial Immunity againstPseudomonas aeruginosaAcute Lung Infection." Infection and Immunity 79, no. 3 (2010): 1289–99. http://dx.doi.org/10.1128/iai.01139-10.

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ABSTRACTMany animal studies investigating adaptive immune effectors important for protection againstPseudomonas aeruginosahave implicated opsonic antibody to the antigenically variable lipopolysaccharide (LPS) O antigens as a primary effector. However, active and passive vaccination of humans against these antigens has not shown clinical efficacy. We hypothesized that optimal immunity would require inducing multiple immune effectors targeting multiple bacterial antigens. Therefore, we evaluated a multivalent live-attenuated mucosal vaccination strategy in a murine model of acuteP. aeruginosapn

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Belaia, O. F., O. A. Paevskaya, S. N. Zuevskaya, et al. "FREQUENCY OF DETECTION OF LPS O-ANTIGENS OF DIFFERENT CAUSATIVE AGENTS IN PATIENTS WITH ACUTE DIARRHEAL DISEASES." Epidemiology and Infectious Diseases 22, no. 2 (2017): 75–79. http://dx.doi.org/10.17816/eid42631.

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The aim is to determine the frequency and dynamics of detection of specific lipopolysaccharide (LPS) O-antigens LPS/O-antigens of causative agents in hospitalized diarrhea patients. Materials and methods. A total of 146 hospitalized diarrhea patients were examined with the use of an immunological method of the reaction of coagglutination (RCA) for the presence of LPS O-antigens of Shigella, Salmonella, Yersinia and Campylobacter in feces as markers of major intestinal infections pathogens. The control group was consisted of 40 blood donors. Results. In acute diarrhea patients there was detecte

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Rifai, Achmad Bahtiar, Anita Yuni Praptiwi, Dwi Sulistiyono, and Titis Wulandari. "TECHNIQUES OF PURIFICATION of ANTIGEN O ISOLATES of Escherichia coli AND IN VIVO ANALYSIS OF ANTIGEN IMMUNOGENICITY IN POULTRY." BIOEDUKASI 21, no. 1 (2023): 1. http://dx.doi.org/10.19184/bioedu.v21i1.28167.

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Lipopolysaccharide (LPS) is a major surface component of most Gram-negative bacteria and is recognized by immune cells as pathogen-associated molecules. Antibodies against O antigens can provide protection against infection and vaccines have been developed by conjugating O antigens to carrier proteins to enhance immunogenicity. This study aims to produce O antigen from Escherichia coli and analyze the in vivo test results in poultry using ELISA technique. The methods used in this study were E. coli Bacterial Culture, O Antigen Purification, SDS PAGE, Inoculation on Experimental Animals, Rapid

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Boissinot, Maurice, Danielle Ramsay, Christine Barthe, and Jean R. Joly. "Antigenic variability of the outer membrane antigens of Legionella pneumophila serogroups 1 to 8." Canadian Journal of Microbiology 33, no. 7 (1987): 607–13. http://dx.doi.org/10.1139/m87-106.

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The outer membrane proteins of Legionella pneumophila serogroups 1 to 8 were prepared from broken cells by selective solubilization using sodium lauryl sarcosinate. The isolated proteins were separated by sodium dodecyl sulfate – polyacrylamide gel electrophoresis and transferred to nitrocellulose sheets. Rabbit antisera against each of the eight serogroups of L. pneumophila were obtained by immunizing each animal with live bacteria. The transferred proteins were revealed using these antisera and peroxidase-labeled swine anti-rabbit immunoglobulins. Antigenic determinants common to all eight s

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M. Soliman, S., H. S. Soliman, H. I. Mohamed, M. A. Salem, and S. A. Ahmed. "Diagnostic performance of RFLP-PCR and sarcosine based indirect ELISA versus immunoassays in Brucella infected and vaccinated small ruminants." BULGARIAN JOURNAL OF VETERINARY MEDICINE 23, no. 3 (2020): 319–30. http://dx.doi.org/10.15547/bjvm.2217.

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This study was carried out for evaluation of the diagnostic performance of different serological assays; buffered acidified plate antigen test (BAPAT), rose bengal plate test (RBPT), immunochromatographic assay (ICA), rivanol test (RivT), indirect ELISA using two types of coating antigens (smooth lipopolysaccharide; S-LPS and N-lauroylsarcosine-extracted antigens; SE) and complement fixation test (CFT). Relative sensitivity and specificity of various techniques were estimated. The traditional serological tests failed to distinguish the vaccinated from naturally infected animals. Using iELISA w

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Chen, Jeng-Chang, Liang-Shiou Ou, Ming-Ling Kuo, et al. "Fetal Macrophages Exposed to Salmonella Antigens Elicit Protective Immunity Against Overwhelming Salmonella Challenge in A Murine Model." Biomedicines 9, no. 3 (2021): 245. http://dx.doi.org/10.3390/biomedicines9030245.

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Despite the evidence for fetal immunization following maternal infection, it remained a mystery how the fetal immune system was primed by vertically-transmitted pathogens or microbial antigens, especially before its full maturation. We previously demonstrated the capacity of fetal macrophages for endocytosing oncoprotein and allergens to bridge towards adaptive immunity in postnatal life. To investigate the immunological consequences of fetal contact with microbial antigens and the role of fetal macrophages in the defense against infection before T-cell development, we exposed gestational day

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Bryan, L. E., T. Schollaardt, C. Y. Pak, C. J. Kim, and J. W. Yoon. "Lack of protection against ascending Escherichia coli pyelonephritis in diabetic rats following immunization with purified lipopolysaccharide." Canadian Journal of Microbiology 32, no. 12 (1986): 967–69. http://dx.doi.org/10.1139/m86-179.

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Purified lipopolysaccharide of Escherichia coli produced specific antibody when injected intraperitoneally or given to rats orally. Either route of immunization did not prevent ascending pyelonephritis in a diabetic rat model. The use of purified LPS excludes the potential contribution of other virulence factors of E. coli as protective antigens in the prevention of ascending pyelonephritis and confirms that anti-lipopolysaccharide antibody is not protective for ascending pyelonephritis.

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Zirk, Nicole M., Saira F. Hashmi, and H. Kirk Ziegler. "The Polysaccharide Portion of Lipopolysaccharide Regulates Antigen-Specific T-Cell Activation via Effects on Macrophage-Mediated Antigen Processing." Infection and Immunity 67, no. 1 (1999): 319–26. http://dx.doi.org/10.1128/iai.67.1.319-326.1999.

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ABSTRACT The lipopolysaccharide (LPS) structure of Salmonella typhimurium has been correlated with the virulence of wild-type strain LT2. Mutants of LT2 with truncated polysaccharide portions of LPS are less virulent than strains with a complete LPS structure. Polyclonal T cells and monoclonal T-cell hybridomas were more reactive to heat-killed rough mutants than to heat-killed smooth strains, as measured by interleukin-2 (IL-2) production. Using a large panel of strains with truncated LPS molecules, we found that T-cell reactivity decreased with certain lengths of polysaccharide. The decrease

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Guillobel, Heloisa C. R., Joana I. Carinhanha, Lucia Cárdenas, John D. Clements, Darcy F. de Almeida, and Luís C. S. Ferreira. "Adjuvant Activity of a Nontoxic Mutant of Escherichia coli Heat-Labile Enterotoxin on Systemic and Mucosal Immune Responses Elicited against a Heterologous Antigen Carried by a LiveSalmonella enterica Serovar Typhimurium Vaccine Strain." Infection and Immunity 68, no. 7 (2000): 4349–53. http://dx.doi.org/10.1128/iai.68.7.4349-4353.2000.

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ABSTRACT Systemic and mucosal antibody responses against both the major subunit of colonization factor antigen I (CFA/I) of enterotoxigenicEscherichia coli (ETEC) and the somatic lipopolysaccharide expressed by recombinant bivalent Salmonella vaccine strains were significantly enhanced by coadministration of a detoxified derivative with preserved adjuvant effects of the ETEC heat-labile toxin, LT(R192G). The results further support the adjuvant effects of LT(R192G) and represent a simple alternative to improve responses against passenger antigens expressed by orally delivered Salmonella vaccin

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Cho, Sang Nae, Jeong Ran Uhm, and Joo Deuk Kim. "Comparative analysis of lipopolysaccharide and lipid antigens ofLeptospira interrogansserovars." Yonsei Medical Journal 33, no. 1 (1992): 24. http://dx.doi.org/10.3349/ymj.1992.33.1.24.

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Ronholm, Jennifer, Zhiyi Zhang, Xundong Cao, and Min Lin. "Monoclonal Antibodies to Lipopolysaccharide Antigens ofSalmonella entericaserotype Typhimurium DT104." Hybridoma 30, no. 1 (2011): 43–52. http://dx.doi.org/10.1089/hyb.2010.0066.

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Cochrane, Doreen M. G., Michael R. W. Brown, and Peter H. Weller. "Lipopolysaccharide antigens produced byPseudomonas aeruginosafrom cystic fibrosis lung infection." FEMS Microbiology Letters 50, no. 2-3 (1988): 241–45. http://dx.doi.org/10.1111/j.1574-6968.1988.tb02945.x.

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Chart, Henrik, and Claire Jenkins. "Salivary antibodies to lipopolysaccharide antigens of Escherichia coli 0157." Lancet 352, no. 9125 (1998): 371. http://dx.doi.org/10.1016/s0140-6736(05)60470-9.

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Domínguez, J. A., N. Galí, P. Pedroso, et al. "Comparison of the Binax Legionella Urinary Antigen Enzyme Immunoassay (EIA) with the Biotest Legionella Urin Antigen EIA for Detection of Legionella Antigen in both Concentrated and Nonconcentrated Urine Samples." Journal of Clinical Microbiology 36, no. 9 (1998): 2718–22. http://dx.doi.org/10.1128/jcm.36.9.2718-2722.1998.

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We evaluated a newly commercial enzyme immunoassay (EIA) (BiotestLegionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophilaserogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity and specificity with those of an EIA for detection ofL. pneumophila serogroup 1 antigen (Legionella urinary antigen EIA; Binax, Portland, Maine). Both tests were performed with both concentrated and nonconcentrated urine samples. We also evaluated the capabilities of both EIAs to de

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Barlow, Avlin K., Xin He, and Charles Janeway. "Exogenously Provided Peptides of a Self-antigen Can Be Processed into Forms that Are Recognized by Self–T Cells." Journal of Experimental Medicine 187, no. 9 (1998): 1403–15. http://dx.doi.org/10.1084/jem.187.9.1403.

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Major histocompatibility complex (MHC) class II molecules can present peptides derived from two different sources. The predominant source of peptide in uninfected antigen presenting cells (APCs) is from self-proteins that are synthesized within the cell and traffic through the MHC class II compartment. The other source of antigen is endocytosed proteins, which includes both self- and foreign proteins. Foreign protein antigens generate adaptive immune responses, whereas self-peptides stabilize the MHC class II heterodimer on the cell surface, allowing positive and negative selection of thymocyt

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Irene, Carmela, Laura Fantappiè, Elena Caproni, et al. "Bacterial outer membrane vesicles engineered with lipidated antigens as a platform for Staphylococcus aureus vaccine." Proceedings of the National Academy of Sciences 116, no. 43 (2019): 21780–88. http://dx.doi.org/10.1073/pnas.1905112116.

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Bacterial outer membrane vesicles (OMVs) represent an interesting vaccine platform for their built-in adjuvanticity and simplicity of production process. Moreover, OMVs can be decorated with foreign antigens using different synthetic biology approaches. However, the optimal OMV engineering strategy, which should guarantee the OMV compartmentalization of most heterologous antigens in quantities high enough to elicit protective immune responses, remains to be validated. In this work we exploited the lipoprotein transport pathway to engineer OMVs with foreign proteins. Using 5 Staphylococcus aure

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Allison, Anthony C., and Noelene E. Byars. "Adjuvants for a New Generation of Vaccines." Canadian Journal of Infectious Diseases 3, suppl b (1992): 84–93. http://dx.doi.org/10.1155/1992/342364.

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Subunit antigens of viruses and other infectious agents in their natural configuration can be produced by recombinant DNA technology. To elicit protective immunity such antigens have to be administered with adjuvants that elicit cell-mediated immunity, including genetically restricted cytotoxicity, and that produce high affinity antibodies of protective isotypes. Antibodies of such isotypes (immunoglobulin G2a [IgG2a] in the mouse and IgG 1 in humans) efficiently activate complement and effect antibody-dependent cell-mediated cytotoxicity. Naturally occurring adjuvants such as lipopolysacchari

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