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Artykuły w czasopismach na temat "Rapd analysis of E. aerogenes"

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Abdul Rahim, Muhammad Khairulanwar, Nur Mas Ayu Jamaludin, Jacinta Santhanam, Azrul Azlan Hamzah, and Muhamad Ramdzan Buyong. "Rapid ESKAPE Pathogens Detection Method using Tapered Dielectrophoresis Electrodes via Crossover Frequency Analysis." Sains Malaysiana 49, no. 12 (2020): 2913–25. http://dx.doi.org/10.17576/jsm-2020-4912-04.

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This paper introduces the versatile of an electrokinetic technique by using the non-uniform electric field for dielectrophoresis (DEP) application. This technique is defined as electromicrofluidics. The potential application for portable and real time detection method of Enterococcus faecium (EF), Staphylococcus aureus (SA), Klebsiella pneumoniae (KP), Acinetobacter baumannii (AB), Pseudomonas aeruginosa (PA) and Enterobacter aerogenes (EA), which are the (ESKAPE) bacteria. The MATLAB analytical modelling was used in simulating the polarisation factor and velocities of bacteria based on Clausi
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Tićac, Mateo, Tanja Grubić Kezele, Maja Abram, and Marina Bubonja-Šonje. "Evaluation of the Microbiological Performance and Potential Clinical Impact of New Rapid Molecular Assays for the Diagnosis of Bloodstream Infections." Microorganisms 13, no. 3 (2025): 616. https://doi.org/10.3390/microorganisms13030616.

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Bloodstream infection (BSI) is a critical medical emergency associated with a high mortality rate. Rapid and accurate identification of the causative pathogen and the results of antimicrobial susceptibility testing are crucial for initiating appropriate antimicrobial therapy. The aim of this study was to evaluate the performance of a new rapid PCR Molecular Mouse System (MMS) for the identification of Gram-negative bacteria (GNB) and GNB resistance genes directly from a positive blood culture (BC). The validation of these rapid multiplex assays was carried out in a real hospital setting. A tot
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Ibraheim, Hanaa, Khadeeja Madhi, Alyaa Jasim, and Hasanain Gharban. "<b>Molecular identification of <i>Klebsiella</i> species from pneumonic goats, Iraq</b>." Open Veterinary Journal 14, no. 11 (2024): 2980. https://doi.org/10.5455/ovj.2024.v14.i11.26.

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&lt;b&gt;Background:&lt;/b&gt; In goats, acute and chronic respiratory infections are often characterized by a rapid progressing clinical course with a little opportunity to develop an effective antibiotic therapy. &lt;b&gt;Aim:&lt;/b&gt; This study aimed to identify Klebsiella spp. in pneumonic goats, assess its antibiotic susceptibility, and confirm the molecular phylogenetics of Klebsiella spp. &lt;b&gt;Methods:&lt;/b&gt; A total of 80 pneumonic goats were selected from the slaughterhouses located in Basra province (Iraq) during June to November 2023, and each animal was subjected to obtain
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Patel, Vipul R., and Nikhil Bhatt. "Isolation, development and identification of salt-tolerant bacterial consortium from crude-oil-contaminated soil for degradation of di-azo dye Reactive Blue 220." Water Science and Technology 72, no. 2 (2015): 311–21. http://dx.doi.org/10.2166/wst.2015.208.

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The objective of this study was development and characterization of a halophilic bacterial consortium for rapid decolorization and degradation of a wide range of dyes and their mixtures. The 16S rRNA gene analysis of developed halophilic consortium VN.1 showed that the bacterial consortium contained six bacterial strains, which were identified as Pseudomonas fluorescens HM480360, Enterobacter aerogenes HM480361, Shewanella sp. HM589853, Arthrobacter nicotianae HM480363, Bacillus beijingensis HM480362 and Pseudomonas aeruginosa JQ659549. Halophilic consortium VN.1 was able to decolorize up to 2
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Ngaffo, Carine M. N., Simplice B. Tankeo, Michel-Gael F. Guefack, et al. "Phytochemical analysis and antibiotic-modulating activity of Cocos nucifera, Glycine max and Musa sapientum methanol extracts against multidrug resistant Gram-negative bacteria." Investigational Medicinal Chemistry and Pharmacology 4, no. 2 (2021): 1–12. http://dx.doi.org/10.31183/imcp.2020.00053.

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Background: The rapid emergence of multidrug resistant (MDR) bacteria is occurring worldwide, endangering the efficacy of antibiotics, which have transformed medicine and saved millions of lives. Antibiotic-resistant infections are already widespread in the Sub-Saharan Africa and across the globe. To extend the search for new and more efficient antimicrobial drugs from natural sources, this work has been carried out to study the phytochemical composition and the antibacterial activities of some Cameroonian dietary plants (Cocos nucifera, Glycine max and Musa sapientum) against several MDR Gram
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HUANG, FANG, SHUANG LI, LAN LOU, JUNJUN MO, and HAO XU. "Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella aerogenes." Polish Journal of Microbiology 70, no. 3 (2021): 409–12. http://dx.doi.org/10.33073/pjm-2021-038.

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Bronchoscopes have been linked to outbreaks of nosocomial infections. The phenotypic and genomic profiles of bronchoscope-associated Klebsiella aerogenes isolates are largely unknown. In this work, a total of 358 isolates and 13 isolates were recovered from samples after clinical procedures and samples after decontamination procedures, respectively, over the five months. Antimicrobial susceptibility testing found seven K. aerogenes isolates exhibiting a low-level resistance to antimicrobial agents. Among seven K. aerogenes isolates, we found five sequence types (STs) clustered into three main
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Choi, Sunghee, Seulgi Kim, Seojin Woo, and Dongju Lee. "Comparative Study of Reduced Graphene Oxide Aerogels and Films for Supercapacitor Electrodes." Journal of Powder Materials 32, no. 1 (2025): 23–29. https://doi.org/10.4150/jpm.2024.00472.

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Supercapacitors, renowned for their high power density and rapid charge-discharge rates, are limited by their low energy density. This limitation has prompted the need for advanced electrode materials. The present study investigated reduced graphene oxide (rGO) in two distinct structures, as a film and as an aerogel, for use as supercapacitor electrodes. The rGO film, prepared by vacuum filtration and thermal reduction, exhibited a compact, lamellar structure, while the aerogel, synthesized through hydrothermal treatment, was a highly porous three-dimensional network. Electrochemical analyses
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Gawel, Nick J., Rory Mellinger, Eric Stout, and R. Sauve. "RAPD Analysis of Acer." HortScience 30, no. 4 (1995): 813F—813. http://dx.doi.org/10.21273/hortsci.30.4.813f.

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DNA from 27 Acer species was used for RAPD analysis. A relatively high number of phylogenically informative polymorphisms were detected, as would be expected in intraspecific comparisons. Principle coordinates analysis was used to discern groupings among the species and a RAPD-based phylogeny was constructed. As expected when making comparisons among species, very high levels of polymorphism were found. Cultivars that grouped together in the principle components analysis also grouped together in the phylogenic analysis. Parts of the phylogenic analysis do not agree with morphology-based phylog
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Tran, Que-Tien, Myrielle Dupont, Jean-Philippe Lavigne та ін. "Occurrence of Efflux Mechanism and Cephalosporinase Variant in a Population of Enterobacter aerogenes and Klebsiella pneumoniae Isolates Producing Extended-Spectrum β-Lactamases". Antimicrobial Agents and Chemotherapy 53, № 4 (2009): 1652–56. http://dx.doi.org/10.1128/aac.00822-08.

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ABSTRACT We investigated the occurrence of multidrug resistance in 44 Enterobacter aerogenes and Klebsiella pneumoniae clinical isolates. Efflux was involved in resistance in E. aerogenes isolates more frequently than in K. pneumoniae isolates (100 versus 38% of isolates) and was associated with the expression of phenylalanine arginine β-naphthylamide-susceptible active efflux. AcrA-TolC overproduction in E. aerogenes isolates was noted. An analysis of four E. aerogenes isolates for which cefepime MICs were high revealed no modification in porin expression but a new specific mutation in the Am
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Wu, Y. J., Y. Chen, J. Wang, C. X. Zhu, and B. L. Xu. "RAPD analysis of jasmine rice-specific genomic structure." Genome 49, no. 6 (2006): 716–19. http://dx.doi.org/10.1139/g06-018.

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Total genomic DNA was extracted from 29 samples of rice seed, including jasmine rice Oryza sativa L. subsp indica 'KDML105', 'KDML105'-derived varieties, nonaromatic Thailand rice, and japonica rice. Polymorphism in RAPD profiles was analyzed to explore the genomic structure specific to jasmine rice. The degree of band sharing was used to evaluate genetic distance between varieties and to construct a phylogenetic tree. RD15, CNTLR85033, and CNT87040 were found to be closest to 'KDML105', which was consistent with the true relation among them. Four RAPD fragments that cooperatively distinguishe
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Rozprawy doktorskie na temat "Rapd analysis of E. aerogenes"

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Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

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Tavale, S. T. "Molecular analysis of wheat genome using ISSR and RAPD markers." Thesis(M.Sc.), CSIR-National Chemical Laboratory, Pune, 2001. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2239.

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Hall, Amanda, Steven Pribanich, and Sean Fox. "Analysis of Reciprocal Inhibition Between Candida albicans and Opportunistic Pathogens Enterobacter aerogenes and Enterobacter cloacae." Digital Commons @ East Tennessee State University, 2020. https://dc.etsu.edu/asrf/2020/presentations/51.

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The fungal pathogen Candida albicans and the opportunistic bacterial pathogens Enterobacter aerogenes and Enterobacter cloacae are common sources of human disease. The colonization of proximal anatomical locations by these pathogens suggests that interspecies polymicrobial interactions between Candida albicans and Enterobacter species occur. In order to understand mechanisms of diseases caused by these pathogens and to further the study of disease prevention, analyzation of their combined activities was conducted in this study. Changes in fungal morphology, cellular viability, and colony densi
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Morgan, Una M. "Molecular characterisation of Cryptosporidium and Giardia using random amplified polymorphic DNA (RAPD) analysis." Thesis, Morgan, Una M. ORCID: 0000-0003-2710-9324 (1995) Molecular characterisation of Cryptosporidium and Giardia using random amplified polymorphic DNA (RAPD) analysis. PhD thesis, Murdoch University, 1995. https://researchrepository.murdoch.edu.au/id/eprint/52235/.

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In this study, genetic variation in two medically important protozoan parasites Cryptosporidium and Giardia, was investigated using the novel PCR-based technique of RAPD (Random Amplified Polymorphic DNA) analysis. This was undertaken in order to obtain fundamental knowledge on the population structure and zoonotic potential of Giardia and Cryptosporidium, both of which are important to fully understand the epidemiology of these parasites. The RAPD technique was also applied to the development of diagnostic PCR primers for Giardia and Cryptosporidium in view of the limitations of currently ava
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Roberts, David Mark. "Genome analysis of plant and insect pathogenic species of Verticillium using molecular DNA methodologies." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313053.

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Weir, Brian James. "Development and application of RAPD analysis for intra- and interspecific characterization within the genus Amelanchier." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/nq23957.pdf.

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Chani, Eduard. "Molecular marker analysis of a segregating monoploid potato family." Diss., Virginia Tech, 1998. http://hdl.handle.net/10919/29792.

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Anther culture experiments were conducted to construct a monoploid family. The donor plants used were hybrids between high leptine producing selections of Solanum chacoense Bitt. and anther culture responsive selections of Solanum phureja Juz. et Buk. Several steps of the anther culture process were studied. The results indicated that genotype remains the main factor affecting anther culture response. Growing anther-donor plants in higher greenhouse temperatures (30 degrees C day/20 degrees C night) increased the number of embryos per anther by 40 percent. A heat shock given to anthers in
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Paterson, Ian. "Molecular genetic (RAPD) analysis of Leach's storm petrels (Oceanodroma leucorhoa) from three breeding islands in Atlantic Canada." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22034.pdf.

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Ahmed, Sabina. "Availability of Nitrogen in Rice-Azolla Dual System and Phylogeny Analysis of Genus Azolla Using RAPD Markers." Kyoto University, 2009. http://hdl.handle.net/2433/124004.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第14692号<br>農博第1774号<br>新制||農||971(附属図書館)<br>学位論文||H21||N4465(農学部図書室)<br>UT51-2009-D404<br>京都大学大学院農学研究科農学専攻<br>(主査)教授 谷坂 隆俊, 教授 稲村 達也, 教授 冨永 達<br>学位規則第4条第1項該当
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陳堅峰 and Kin-fung Chan. "Phylogenetic relationships and genetic diversity detected by rapd and isozyme analysis of crop and weedy species of amaranthus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1996. http://hub.hku.hk/bib/B29803846.

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Książki na temat "Rapd analysis of E. aerogenes"

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Gabino García de los Santos. Crossing behavior, RAPD analysis and chlorophyll flourescence in relationship to the geographic adaption of birdsfoot trefoil (Lotus corniculatus L.). 1997.

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Części książek na temat "Rapd analysis of E. aerogenes"

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Tingey, Scott V., J. Antoni Rafalski, and Michael K. Hanafey. "Genetic Analysis with RAPD Markers." In Plant Molecular Biology. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78852-9_45.

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Taylor, P. W. J., T. A. Fraser, H. L. Ko, and R. J. Henry. "RAPD Analysis of Sugarcane During Tissue Culture." In Current Issues in Plant Molecular and Cellular Biology. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0307-7_32.

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Kornacker, M. G., A. Boyd, and G. S. Plastow. "A Genetic Analysis of Pullulanase Export from Klebsiella Aerogenes." In Membrane Biogenesis. Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73184-6_28.

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Saunders, G. C., and S. J. Owens. "RAPD and ITS Analysis of Orchid Mycorrhizal Fungi." In Mycorrhiza Manual. Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-60268-9_26.

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Fujimori, F., H. Takaya, Y. Anzai, and T. Okuda. "RAPD and PAUP Analysis for Microbial Screening Programs." In Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_25.

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Bin, Wang, Li Songtao, Zhang Zhongting, Bao Xiaoming, and Huang Baiqu. "Identification of Wheat-Wheatgrass Translocation Lines by RAPD Analysis." In Biotechnology in Agriculture. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1779-1_39.

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Mikhailova, N., and K. Johannesson. "A comparison of different protocols for RAPD analysis of Littorina." In Aspects of Littorinid Biology. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5336-2_5.

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Waugh, R. "RAPD Analysis: Use for Genome Characterization, Tagging Traits and Mapping." In Plant Molecular Biology — A Laboratory Manual. Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-87873-2_6.

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Black, William C., and Nancy M. DuTeau. "RAPD-PCR and SSCP analysis for insect population genetic studies." In The Molecular Biology of Insect Disease Vectors. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-1535-0_31.

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Graham, G. C., and R. J. Henry. "Preparation of Fungal Genomic DNA for PCR and RAPD Analysis." In Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_5.

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Streszczenia konferencji na temat "Rapd analysis of E. aerogenes"

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Varshini, R. Amirtha, T. Sathishkumar, K. Kumaresan, and A. Thirumurugan. "In silico sequence analysis of urease from Klebsiella aerogenes." In THE 8TH ANNUAL INTERNATIONAL SEMINAR ON TRENDS IN SCIENCE AND SCIENCE EDUCATION (AISTSSE) 2021. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0108173.

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Aputri, Farah Nuriessa, Laila Hanum, Rahmat Pratama, and Yuanita Windusari. "Analysis of Mosquito Genetic with PCR-RAPD Approach." In 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce). IEEE, 2021. http://dx.doi.org/10.1109/inhence52833.2021.9537231.

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Besedina, E. N., V. I. Kil, and M. N. Karpunin. "UNIVERSAL RAPD AND ISSR PRIMERS FOR LACE BUGS PCR ANALYSIS (HETEROPTERA: TINGIDAE)." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS. DSTU-PRINT, 2020. http://dx.doi.org/10.23947/interagro.2020.1.393-396.

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46 RAPD and 25 ISSR primers were tested for universality and specificity for DNA of bugs of the lace family (Heteroptera, Tingidae). Universal RAPD primers (OPA 07, 09, 18), with high specificity and revealing genetic polymorphism in lace bugs were revealed. These primers can be used for intraspecific and interspecific comparisons of the studied bugs
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Shahimi, Safiyyah, Sahilah Abdul Mutalib, Rozida Abdul Khalid, et al. "Genome analysis and identification of gelatinase encoded gene in Enterobacter aerogenes." In THE 2016 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Author(s), 2016. http://dx.doi.org/10.1063/1.4966769.

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"Molecular analysis of sugar beet samples using the RAPD method." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-001.

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Nurhasanah, Ade Nena, Eva Erdayani, Vincentia Esti Windiastri, Ina Erlinawati, Dwi Widyajayantie, and Satya Nugroho. "RAPD analysis of fifteen accessions of Typhonium in Java Island." In INTERNATIONAL CONFERENCE ON ORGANIC AND APPLIED CHEMISTRY (ICOAC) 2022. AIP Publishing, 2024. http://dx.doi.org/10.1063/5.0188654.

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Aloysius, Suyitno, Ixora S. Mercuriani, Ratnawati, and Sudarsono. "Molecular Characterization of Orchid Variants Spathoglottis plicata Blume Based on RAPD Analysis." In 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020). Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210305.009.

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Auvira, Fay Della Prika, Ixora Sartika Mercuriani, and Suyitno Aloysius. "Genetic Variability Analysis of Terrestrial Spathoglottis plicata Orchid Variants Based on RAPD Marker." In 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020). Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210305.011.

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Sianipar, Nesti Fronika, Reflinur, Muhammad Dzulkifly Ashan, Khoirunnisa Assidqi, Dwityantari Widyaningrum, and Ragapadmi Purnamaningsih. "Direct verification of bogor superior mutant (Typhonium flagelliforme) using RAPD marker techniques and sequencing analysis." In THE 8TH INTERNATIONAL CONFERENCE AND WORKSHOP ON BASIC AND APPLIED SCIENCE (ICOWOBAS) 2021. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0104057.

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Ifah, Arini Al, Endang Yuniastuti, and Parjanto. "Analysis of breadfruit plant diversity (Artocarpus altilis P.) by random amplified polymorphic DNA (RAPD) in DIY." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062802.

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Raporty organizacyjne na temat "Rapd analysis of E. aerogenes"

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Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim, and Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613014.bard.

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Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and be
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ทัศนาขจร, อัญชลี. การศึกษาความแตกต่างแปรผันทางพันธุกรรมในประชากรกุ้งกุลาดำ โดยการตรวจลายพิมพ์ดีเอ็นเอ ด้วยตัวตรวจสอบที่มีลำดับเบสซ้ำแบบง่าย : รายงานวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์์มหาวิทยาลัย, 1997. https://doi.org/10.58837/chula.res.1997.19.

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ในการศึกษาความแตกต่างแปรผันทางพันธุกรรมในประชากรกุ้งกุลาดำ ได้ทดสอบวิธีการตรวจลายพิมพ์ดีเอ็นเอในกุ้งกุลาดำและได้เลือก 2 วิธี เพื่อใช้ในการศึกษาความแตกต่างแปรผันทางพันธุกรรม คือ 1. การตรวจลายพิมพ์ดีเอ็นเอ โดยเทคนิค RAPD (Random Amplified Polymorphic DNA analysis) และ 2. การตรวจความแปรผันของ microsatellites DNA ในการศึกษานี้ได้เก็บตัวอย่างกุ้งจากแหล่งที่นิยมใช้เป็นพ่อ-แม่พันธุ์ในการเพาะเลี้ยงกุ้ง จำนวน 5 แหล่ง โดยเก็บตัวอย่างจากทะเลอันดามัน 3 แหล่ง ได้แก่ กุ้งบริเวณ จ. สตูล-ตรัง กุ้งจากจ. พังงา และกุ้งจากเมดาน ประเทศอินโดนีเซีย และเก็บตัวอย่างกุ้งจากฝั่งอ่าวไทย 2 แหล่ง ได้แก่ กุ้งจาก จ. ตราด และ
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Sadka, Avi, Mikeal L. Roose, and Yair Erner. Molecular Genetic Analysis of Citric Acid Accumulation in Citrus Fruit. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7573071.bard.

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The acid content of the juice sac cells is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Pulp acidity is thought to be dependent on two mechanisms: the accumulation of citric acid in the vacuoles of the juice sac cells, and acidification of the vacuole. The major aim of the project was to direct effort toward understanding the mechanism of citric acid accumulation in the fruit. The following objectives were suggested: Measure the activity of enzym
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Veilleux, Richard E., Jossi Hillel, A. Raymond Miller, and David Levy. Molecular Analysis by SSR of Genes Associated with Alkaloid Synthesis in a Segregating Monoploid Potato Family. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7570550.bard.

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More than 15,000 anthers of an interspecific hybrid (CP2) between two diploid (2n=2x=24) potato species, Solanum chacoense (weedy) and S. phureja (cultivated), were cultured to generate a family of monoploid (haploid, 2n-1x=12) plants. Of 260 regenerated plants, 34 were monoploid, 210 diploid and 16 tetraploid. SSR analysis revealed that six monoploids were genetically identical and 14 diploids were homozygous, thus limiting the population to 42 (28 monoploids and 14 homozygous diploids). New microsatellite loci were developed for potato from database sequences (15), a conventional genomic lib
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Kistler, Harold Corby, Talma Katan, and Dani Zamir. Molecular Karyotypes of Pathogeic Strains of Fusarium oxysporum. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604927.bard.

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Genetic diversity of pathogenic strains of the fungus Fusarium oxysporum was determied by analysis of electrophoretic karyotype, as well as by DNA variation detected by Restriction Fragment Length Polymorphisms (RFLPs) and Random Amplified Polymorphic DNAs (RAPDs). The electrophoretic karyotypes for 130 isolates of the fungus pathogenic to tomato, melon, and banana were analyzed. Electrophoretic karyotype variation, reflected in differences in apparent chromosome number and genome size, was observed even among isolates from the same host and sub specific category. Sub specific categories studi
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Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular mark
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Amzeri, Achmad, Kaswan Badami, and Gita Pawana. Inheritance of resistance to downy mildew (Peronosclerospora maydis) in crossing of Madura Maize Plant (Zea mays L.). Innovative Scientific Information & Services Network, 2019. http://dx.doi.org/10.21107/amzeri.2019.1.

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Hybridization of Back cross is one method to get varieties that are resistant to downy mildew. The purpose of this study was to obtain information on inheritance characteristics of downy mildew resistance. This research was conducted at the experiment center of Agro-Technology Study Program of Agriculture Faculty, University of Trunojoyo Madura. Research of Assessment of resistance to Downy Mildew used a randomized block design with 18 treatments (P1, P2, F1, F2, BC1P1 and BC1P2 in three sets of crosses, namely LGL x Mdr-3, T12 x Mdr-1 and E02 x Mdr-2) and three replications so there were 54 e
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Freeman, Stanley, and Daniel Legard. Epidemiology and Etiology of Colletotrichum Species Causing Strawberry Diseases. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7695845.bard.

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Diseases caused by Colletotrichum spp. are one of the most important limitations on international strawberry production, affecting all vegetative and fruiting parts of the plant. From 1995 to 1997, C. acutatum infections reached epidemic levels in Israeli strawberry nurseries, causing extensive loss of transplants in fruit-bearing fields and additional reductions in yield. Although C. acutatum also occurs on strawberry in Florida, recent crown rot epidemics have been primarily caused by C. gloeosporioides. Little is known about the basic epidemiology of these important diseases on strawberry.
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Katzir, Nurit, Rafael Perl-Treves, and Jack E. Staub. Map Merging and Homology Studies in Cucumis Species. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575276.bard.

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List of original objectives (1) Construct a saturated map of melon, using RFLP, SSR, RAPD and Inter-SSR genetic markers. (2) Study the homology between the genomes of cucumber and melon. (3) Add to the Cucumis map, biologically important genes that had been cloned in other plant systems. Background Cucumber and melon are important vegetable crops in Israel and the US. Genome analysis of these crops has lagged behind the major plant crops, but in the last few years genetic maps with molecular markers have been developed. The groups that participated in this program were all involved in initial
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Droby, Samir, Joseph W. Eckert, Shulamit Manulis, and Rajesh K. Mehra. Ecology, Population Dynamics and Genetic Diversity of Epiphytic Yeast Antagonists of Postharvest Diseases of Fruits. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568777.bard.

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One of the emerging technologies is the use of microbial agents for the control of postharvest diseases of fruits and vegetables. A number of antagonistic microorganisms have been discovered which have the potential to effectively control postharvest diseases. Some of this technology has been patented and commercial products such as AspireTM (Ecogen Corporatin, Langhorne, PA, USA), Biosave 10TM and Biosave 11TM (Ecoscience Inc., Worchester, MA, USA) have been registered for commercial use. The principal investigator of this project was involved in developing the yeast-based biofungicide-Aspire
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