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Chiykowski, L. N. "BIOLOGY AND REARING OF PARAPHLEPSIUS IRRORATUS (HOMOPTERA: CICADELLIDAE), A VECTOR OF PEACH X-DISEASE." Canadian Entomologist 117, no. 6 (June 1985): 717–26. http://dx.doi.org/10.4039/ent117717-6.
Повний текст джерелаАнотація:
AbstractA method of rearing Paraphlepsius irroratus (Say) under artificial conditions was developed by using a leguminous plant species as the oviposition host and a gramineous species as the food plant for nymphs. Nymphs completed their development on all 10 gramineous species tested. Total time for completion of the 5 nymphal instars ranged from 31.8 to 59.2 days, depending on the plant species used. With the exception of Alopecurus pratensis, all monocotyledonous species tested were found to be suitable food plants for nymphs, allowing 50% or more of the insects to survive for 3 weeks or more. Only 5 dicotyledonous species supported a similar survival level of nymphs. Adult survival remained above the 50% level on Apium graveolans var. dulce, 6 members of the Leguminosae, and all members of Composite with the exception of Callistephus chinensis. Survival of adult leafhoppers on monocotyledons was relatively high after 1 week but considerable mortality followed longer confinement. Although nymphs emerged from eggs laid on a wide range of plant species, the numbers recorded on monocotyledons suggest that monocots are important as oviposition hosts for P. irroratus in nature.
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Weston, Leslie A., Jacob N. Barney, and Antonio DiTommaso. "A Review of the Biology and Ecology of Three Invasive Perennials in New York State: Japanese Knotweed (Polygonum cuspidatum), Mugwort (Artemisia vulgaris) and Pale Swallow-wort (Vincetoxicum rossicum)." Plant and Soil 277, no. 1-2 (December 2005): 53–69. http://dx.doi.org/10.1007/s11104-005-3102-x.
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Yamada, Masashi, Nobuyasu Matsuura, Hiroto Suzuki, Chihiro Kurosaka, Naoko Hasegawa, Makato Ubukata, Toshiyuki Tanaka, and Munekazu Iinuma. "Corrigendum to “Germacranolides from Calea urticifolia” [Phytochem. 65 (2004) 3107–3111]." Phytochemistry 66, no. 15 (August 2005): 1864. http://dx.doi.org/10.1016/j.phytochem.2005.05.014.
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Chan, Charlotte R., and Robert D. Marquard. "Horticultural Research at The Holden Arboretum." HortScience 31, no. 4 (August 1996): 627e—627. http://dx.doi.org/10.21273/hortsci.31.4.627e.
Повний текст джерелаАнотація:
The Holden Arboretum, established in 1931, is the largest arboretum in the United States. Its mission is to promote the knowledge and appreciation of plants for personal enjoyment, inspiration, and recreation; for scientific research; and for educational and aesthetic purposes. Of the Arboretum's 3100 acres, 800 acres support collections and display gardens, while the balance comprise natural areas. The collections include nearly 8,000 accessions from 76 plant families; about 700 plant species, some rare or endangered, occupy the natural areas. The education component of the mission connects the Arboretum with the public through school programs, classes, horticultural therapy, and seasonal internships. Two research fellowships are also available. The Holden Arboretum has expanded the research emphasis. The David G. Leach Research Station, part of the Arboretum since 1986, focuses on rhododendron and magnolia breeding and research. Built in 1993, the Horticulture Science Center is a modern research and production facility able to more fully implement and support a broad range of formal horticultural research. The main objective of the research program is to develop superior woody ornamentals for the landscape through hybridization. Additional research emphasizes reproductive biology and using biochemical markers (isozymes and RAPDs) to answer basic questions about the genera under study (Aesculus, Hamamelis, Cercis).
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Djurovic, Sanja, Vesna Dragicevic, Hadi Waisi, Maja Pagnacco, Nevena Lukovic, Zorica Knezevic-Jugovic, and Bogdan Nikolic. "Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products." Archives of Biological Sciences 71, no. 3 (2019): 425–34. http://dx.doi.org/10.2298/abs190123024d.
Повний текст джерелаАнотація:
Polyphenols present in different plant cell organelles increase the resistance of plants to various types of environmental stresses. We investigated the possibility of increasing the content of bioactive compounds in the seed of yellow soybean variety Laura. The soybean was treated during vegetation with five products based on plant extracts, on the assumption of enrichment of plants with various nutrients. Soybean flour extracts were screened spectrophotometrically for total phenolic content and antioxidant activity. The antioxidant activity was evaluated using three methods. The content of phenolic acids was determined by HPLC, and the raw protein content was estimated by the Kjeldahl method. Depending on the treatment, variations in the quantity of individual phenolic acids with up to 90% higher concentration as compared to the control were observed. Controlled usage of certain plant extracts can increase the concentration of the target group of bioactive compounds in the samples. The synergistic effect of proteins and phenolic compounds on the antioxidant activity of extracts was detected. The results of this study are not only important from the aspect of plant resistance to various types of stress, but also when considering soybean as a functional food. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31018, Grant no. 31037, Grant no. 37021 and Grant no. 172015]
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Huang, Yuanyuan, Zhansheng Wu, Yanhui He, Bang-Ce Ye, and Chun Li. "RhizosphericBacillus subtilisExhibits Biocontrol Effect againstRhizoctonia solaniin Pepper(Capsicum annuum)." BioMed Research International 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/9397619.
Повний текст джерелаАнотація:
This study aimed at evaluating the ability of SL-44 to controlRhizoctonia solaniand promote pepper(Capsicum annuum)growth. Strain SL-44 was isolated from plant rhizosphere and the pot experiment results indicated that the dry and fresh weights of pepper in SL-44 andRhizoctonia solani(S-R) treatment were 45.5% and 54.2% higher than those inRhizoctonia solani(R) treatment and 18.2% and 31.8% higher than those in CK (control, noninoculation) treatment. The plant height in S-R treatment increased by 14.2% and 9.0% compared with those in the R and CK treatments, respectively. In vitro antagonism assay showed that SL-44 exhibited strong antifungal activity against the mycelial growth ofRhizoctonia solani, with an inhibition rate of 42.3%. The amount of phosphorus dissolved by SL-44 reached 60.58 mg·L−1in broth and 7.5 μg·mL−1IAA were secreted by SL-44. Strain SL-44 inhibited the growth ofR. solaniand improved biomass of pepper plants. Mass exchange and information transmission between the pepper plants and SL-44 mutually promoted their development.Bacillus subtilisSL-44 has a great potential as biocontrol agent againstRhizoctonia solanion pepper plants.
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Peterson, Richard B. "PsbS genotype in relation to coordinated function of PS II and PS I in Arabidopsis leaves." Photosynthesis Research 85, no. 2 (August 2005): 205–19. http://dx.doi.org/10.1007/s11120-005-3106-7.
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Gennis, Robert B. "Respiration in Archaea and Bacteria: Diversity of Prokaryotic Electron Transport Carriers. Davide Zannoni, Advances in Photosynthesis and Respiration (Series Editor, Govindjee), Kluwer Academic Publishers, Dordrecht, The Netherlands, Volume 15, 2004, 350 pp, ISBN 1-4020-2001-5, Price EUR 175.00, USD 193.00, GBP 121.00." Photosynthesis Research 83, no. 3 (March 2005): 363–64. http://dx.doi.org/10.1007/s11120-005-3107-6.
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Nair, N., D. Plant, J. Isaacs, A. Morgan, K. Hyrich, A. Barton, and A. G. Wilson. "AB0011 DNA METHYLATION AS A BIOMARKER OF TOCILIZUMAB RESPONSE IN RHEUMATOID ARTHRITIS PATIENTS." Annals of the Rheumatic Diseases 81, Suppl 1 (May 23, 2022): 1141.1–1141. http://dx.doi.org/10.1136/annrheumdis-2022-eular.3102.
Повний текст джерелаАнотація:
BackgroundTocilizumab (TCZ) is a disease-modifying antirheumatic biologic drug, which targets the IL-6 signalling pathway and is effective in ameliorating disease activity in rheumatoid arthritis (RA). However, approximately 50% of patients do not respond adequately to TCZ and some patients report adverse events. Considering there is growing evidence that DNA methylation is implicated in RA susceptibility and response to some biologics (1, 2), we investigated DNA methylation as a candidate biomarker for response to TCZ in RA.ObjectivesTo identify differential DNA methylation signatures in whole blood associated with TCZ response in patients with RA.MethodsEpigenome-wide DNA methylation patterns were measured using the Infinium EPIC 850k BeadChip (Illumina) in whole blood-derived DNA samples from patients with RA. DNA was extracted from blood samples taken pre-treatment and following 3 months on therapy, and response was determined at 6 months using the Clinical Disease Activity Index (CDAI). Patients who had good response (n=10) or poor response (n=10) to TCZ by 6 months were selected. Samples from secondary poor responders (n=10) (patients who had an improvement of CDAI and were in remission at 3 months, followed by a worsening of CDAI at 6 months) were also analysed. Differentially methylated positions (DMPs) were identified using linear regression, adjusting for gender, age, cell composition, smoking status, and glucocorticoid use.ResultsIn the pre-treatment samples, 20 DMPs were significantly associated with response status at 6 months (unadjusted p-value <10-6), whilst in the 3 month samples, 21 DMPs were associated with response. One DMP, cg03121467, was significantly less methylated in good responders compared to poor responders in the pre-treatment samples. This DMP is close to EPB41L4A and may play a role in β–catenin signalling. Interestingly, cg10136146 was significantly less methylated in secondary poor responders compared to both good and poor responders in the 3 month samples. This DMP maps close to CD81, which plays a role in mediating the development and activation of B and T lymphocytes.ConclusionThese preliminary results provide evidence that DNA methylation patterns may predict response to TCZ. Further regional and pathway analyses is in progress and validation of these findings in other larger data sets is required.References[1]Liu,Y., Aryee,M.J., Padyukov,L., Fallin,M.D., Hesselberg,E., Runarsson,A., Reinius,L., Acevedo,N., Taub,M., Ronninger,M., et al. (2013) Epigenome-wide association data implicate DNA methylation as an intermediary of genetic risk in rheumatoid arthritis. Nat. Biotechnol., 31, 142–147.[2]Plant,D., Webster,A., Nair,N., Oliver,J., Smith,S.L., Eyre,S., Hyrich,K.L., Wilson,A.G., Morgan,A.W., Isaacs,J.D., et al. (2016) Differential Methylation as a Biomarker of Response to Etanercept in Patients With Rheumatoid Arthritis. Arthritis Rheumatol. (Hoboken, N.J.), 68, 1353–60.Disclosure of InterestsNisha Nair: None declared, Darren Plant: None declared, John Isaacs Speakers bureau: Abbvie, Gilead, Roche, UCB, Grant/research support from: GSK, Janssen, Pfizer, Ann Morgan Speakers bureau: Roche/Chugai, Consultant of: GSK, Roche, Chugai, AstraZeneca, Regeneron, Sanofi, Vifor, Grant/research support from: Roche, Kiniksa Pharmaceuticals, Kimme Hyrich Consultant of: AbbVie, Grant/research support from: Pfizer, BMS, Anne Barton Grant/research support from: I have received grant funding from Pfizer, Galapagos, Scipher Medicine and Bristol Myers Squibb., Anthony G Wilson: None declared
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Keerthana, K., S. Chitra, and T. Naveenkumar. "Screening of finger millet genotypes for sodicity tolerance using the Na+/K+ ratio as a major physiological trait." Journal of Applied and Natural Science 14, SI (July 15, 2022): 73–76. http://dx.doi.org/10.31018/jans.v14isi.3571.
Повний текст джерелаАнотація:
Sodicity affects a larger area than salinity, but research on the sodicity tolerance mechanism is limited. The study was carried out to screen 120 finger millet genotypes under sodic soil conditions and identify sodicity-tolerant genotypes. The experimental field soil conditions were sandy clay loam with pH 8.9, electrical conductivity (EC) 0.94 dSm-1 and exchangeable sodium percentage (ESP) 21.5, which was naturally sodic. Grain yield per plant and Na+/K+ ratio were recorded for each genotype to screen sodicity tolerance among the genotypes. A significantly higher grain yield per plant than that of the sodicity-tolerant check variety TRY 1 (23.10 g) was observed in 30 finger millet genotypes. The analysis of sodium and potassium revealed that these 30 finger millet genotypes also recorded a significantly lower Na+/K+ ratio, which is comparatively lower than that of the sodicity-tolerant check variety TRY 1 (0.23 Na+/K+ ratio). The genotypes (FIN 3045, FIN 2875, FIN 3077, FIN 3015, FIN 3063, FIN 2861, FIN 3028, FIN 2867, FIN 2854, FIN 2860, FIN 2872, FIN 2896, FIN 4268, FIN 3034, FIN 3928, FIN 3104, FIN 3965, FIN 3091, FIN 2960, FIN 3994, FIN 4198, FIN 3174, FIN 3078, FIN 4288, FIN 4202, FIN 4238, FIN 3089, FIN 4205, FIN 3966 and FIN 3182) that recorded higher grain yield per plant and lower Na+/K+ ratio can be considered sodicity tolerant. These genotypes with a high grain yield per plant and a low Na+/K+ ratio could be utilized in stress breeding programs to develop sodicity-tolerant finger millet varieties.
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Lan, Ping, Wenfeng Li, and Wolfgang Schmidt. "Complementary Proteome and Transcriptome Profiling in Phosphate-deficient Arabidopsis Roots Reveals Multiple Levels of Gene Regulation." Molecular & Cellular Proteomics 11, no. 11 (July 25, 2012): 1156–66. http://dx.doi.org/10.1074/mcp.m112.020461.
Повний текст джерелаАнотація:
Phosphate (Pi) deficiency impairs plant growth and productivity in many agricultural ecosystems, causing severe reductions in crop yield. To uncover novel aspects in acclimation to Pi starvation, we investigated the correlation between Pi deficiency-induced changes in transcriptome and proteome profiles in Arabidopsis roots. Using exhaustive tandem mass spectrometry-based shotgun proteomics and whole-genome RNA sequencing to generate a nearly complete catalog of expressed mRNAs and proteins, we reliably identified 13,298 proteins and 24,591 transcripts, subsets of 356 proteins and 3106 mRNAs were differentially expressed during Pi deficiency. Most dramatic changes were noticed for genes involved in Pi acquisition and in processes that either liberate Pi or bypass Pi/ATP-consuming metabolic steps, for example during membrane lipid remodeling and glycolytic carbon flux. The concordance between the abundance of mRNA and its encoded protein was generally high for highly up-regulated genes, but the analysis also revealed numerous discordant changes in mRNA/protein pairs, indicative of divergent regulation of transcription and post-transcriptional processes. In particular, a decreased abundance of proteins upon Pi deficiency was not closely correlated with changes in the corresponding mRNAs. In several cases, up-regulation of gene activity was observed solely at the protein level, adding novel aspects to key processes in the adaptation to Pi deficiency. We conclude that integrated measurement and interpretation of changes in protein and transcript abundance are mandatory for generating a complete inventory of the components that are critical in the response to environmental stimuli.
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Elangovan, S., Y. H. Kwan, and W. Fong. "SAT0371 ARE ENGLISH-LANGUAGE VIDEOS ON YOUTUBE A USEFUL SOURCE OF INFORMATION FOR SPONDYLOARTHRITIS?" Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 1133.1–1133. http://dx.doi.org/10.1136/annrheumdis-2020-eular.3109.
Повний текст джерелаАнотація:
Background:Spondyloarthritis (SpA) is a family of chronic inflammatory disorders. Social media, such as YouTube, is a popular online platform where patients often visit for information. However, the validity of the content uploaded onto YouTube is not known.Objectives:This study aimed to evaluate the content, reliability and quality of the most viewed English-language YouTube videos on SpA.Methods:Keywords “spondyloarthritis”, “spondyloarthropathy” and “ankylosing spondylitis” were searched on YouTube on October 7th, 2019. The top 270 videos were screened. Videos were excluded if they were irrelevant, in non-English language or if they had no audio. Total number of views, duration on YouTube (days), video length, upload date, number of likes, dislikes, subscribers and comments were recorded for videos. A modified 5-point DISCERN tool1and the 5-point Global Quality Scale (GQS) score2were used to assess the reliability and quality of the videos, with higher scores indicating greater reliability and quality respectively.Results:Two hundred of 270 videos were included in the final analysis [61.5% from healthcare professionals, 37.0% from patients, 1.5% from news channels]. Of the 200 videos, 15 were uploaded within the last year and 112 in the last five years. 120 (60%) were categorized as useful information (Group 1), 6 (3%) as misleading information (Group 2), 52 (26%) as useful patient opinion (Group 3) and 22 (11%) as misleading patient opinion (Group 4). Useful videos were mainly from healthcare professionals or patients (86%). Useful videos (Group 1 and 3) had higher median (IQR) number of subscribers [2700 (14700) vs 211 (457), p < 0.01], reliability scores [3 (1) vs 2 (1), p < 0.01] and GQS scores [3 (1) vs. 2 (1), p < 0.001] compared to misleading videos (Group 2 and 4), respectively.Videos uploaded by healthcare professionals tended to have more useful information [94% (116 of 123) vs. 66% (49 of 74), p < 0.001] and had higher median (IQR) reliability scores [3 (1) vs 2 (1), p < 0.001] and GQS scores [3 (2) vs 2 (1), p < 0.001] compared to patient uploaded videos respectively. Of the 5 (out of 123) videos from healthcare professionals that had misleading information, it was because of outdated information on diagnosis (3 videos) and treatment (5 videos) of SpA. Of the 22 videos that had misleading patient opinion, 9 (41%) wrongly described the clinical features for SpA and 14 (64%) portrayed the current evidence based treatment options as ineffective and described alternative treatment plans (i.e. diet restrictions, complementary and alternative medicine).Conclusion:The majority of English language YouTube videos have useful information on the topic of SpA, however, 31% of patient opinions have inaccurate information on the clinical features and treatment options, and viewers need to be cognisant of these “fake news”.References:[1]Charnock D, Shepperd S, Needham G, Gann R (1999) DISCERN: an instrument for judging the quality of written consumer health information on treatment choices. J Epidemiol Community Health 53(2): 105-111[2]Bernard A, Langille M, Hughes S, Rose C, Leddin D, Veldhuyzen van Zanten S (2007) A systematic review of patient inflammatory bowel disease information resources on the World Wide Web. Am J Gastroenterol 102(9):2070-2077Disclosure of Interests:Sakktivel Elangovan: None declared, Yu Heng Kwan: None declared, Warren Fong Consultant of: Abbvie, Janssen, Novartis, Speakers bureau: Abbvie, Janssen, Novartis
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Tannuri, Luciano Abi Rached, Everaldo Antônio Lopes, Willian Rodrigues Macedo, and Ellen Júnia Canedo. "Exogenous application of salicylic acid to control coffee rust." Acta Scientiarum. Biological Sciences 43 (March 23, 2021): e54495. http://dx.doi.org/10.4025/actascibiolsci.v43i1.54495.
Повний текст джерелаАнотація:
The exogenous application of salicylic acid can induce plant resistance against pathogens. However, little is known about the potential uses of this bioregulator for controlling coffee diseases. In this study, we assessed the effect of applying salicylic acid (SA – 150 mg L-1) on the management of coffee rust (Hemileia vastatrix) in a 7-year-old coffee plantation with low crop load (651.6 kg ha-1 in 2017). For comparison, plants were sprayed with protectant fungicide (copper hydroxide – CH) and standard fungicides (SF) used by local farmers (boscalid, pyraclostrobin + epoxiconazole, and copper hydroxide). Non-treated plants were included as a negative control. Five monthly applications were performed from November 2016 to March 2017. Rust incidence and severity, defoliation, and growth of plagiotropic branches were evaluated monthly. The activity of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and total proteins was assessed one day after the first, third, and fifth product applications. Compared to untreated plants, SA reduced the severity and incidence of rust from 36.3 to 54.7%, while CH and SF reduced disease from 31.8 to 54.6% and from 83.8 to 88%, respectively. SA reduced defoliation by 54.1%. SA increased the concentration of CAT, APX, and SOD after the first application. However, this effect was not observed after subsequent applications. Foliar application of SA reduces the severity and incidence of coffee rust and defoliation in plants with a low crop load.
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McPherson, Brett, Mihray Sharip, and Terry Grimmond. "The impact on life cycle carbon footprint of converting from disposable to reusable sharps containers in a large US hospital geographically distant from manufacturing and processing facilities." PeerJ 7 (February 22, 2019): e6204. http://dx.doi.org/10.7717/peerj.6204.
Повний текст джерелаАнотація:
Background Sustainable purchasing can reduce greenhouse gas (GHG) emissions at healthcare facilities (HCF). A previous study found that converting from disposable to reusable sharps containers (DSC, RSC) reduced sharps waste stream GHG by 84% but found transport distances impacted significantly on GHG outcomes and recommended further studies where transport distances are large. This case-study examines the impact on GHG of nation-wide transport distances when a large US health system converted from DSC to RSC. Methods The study’s scope was to examine life cycle GHG emissions during 12 months of facility-wide use of DSC and RSC at Loma Linda University Health (LLUH). The facility is an 1100-bed US, 5-hospital system where: the source of polymer was distant from the RSC manufacturing plant; both manufacturing plants were over 3,000 km from the HCF; and the RSC processing plant was considerably further from the HCF than was the DSC disposal plant. Using a “cradle to grave” life cycle GHG tool we calculated the annual GHG emissions of CO2, CH4 and N2O expressed in metric tonnes of carbon dioxide equivalents (MTCO2eq) for each container system. Primary energy input data was used wherever possible and region-specific energy-impact conversions were used to calculate GHG of each unit process over a 12-month period. The scope included Manufacture, Transport, Washing, and Treatment & disposal. GHG emissions from all unit process within these four life cycle stages were summed to estimate each container-system’s carbon footprint. Emission totals were workload-normalized and analysed using CHI2test with P ≤ 0.05 and rate ratios at 95% CL. Results Converting to RSC, LLUH reduced its annual GHG by 162.4 MTCO2eq (−65.3%; p < 0.001; RR 2.27–3.71), and annually eliminated 50.2 tonnes of plastic DSC and 8.1 tonnes of cardboard from the sharps waste stream. Of the plastic eliminated, 31.8 tonnes were diverted from landfill and 18.4 from incineration. Discussion Unlike GHG reduction strategies dependent on changes in staff behavior (waste segregation, recycling, turning off lights, car-pooling, etc), purchasing strategies can enable immediate, sustainable and institution-wide GHG reductions to be achieved. This study confirmed that large transport distances between polymer manufacturer, container manufacturer, user and processing facilities, can significantly impact the carbon footprint of sharps containment systems. However, even with large transport distances, we found that a large university health system significantly reduced the carbon footprint of their sharps waste stream by converting from DSC to RSC.
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He, Ziqing, Yingjuan Su, and Ting Wang. "Full-Length Transcriptome Analysis of Four Different Tissues of Cephalotaxus oliveri." International Journal of Molecular Sciences 22, no. 2 (January 14, 2021): 787. http://dx.doi.org/10.3390/ijms22020787.
Повний текст джерелаАнотація:
Cephalotaxus oliveri is a tertiary relict conifer endemic to China, regarded as a national second-level protected plant in China. This species has experienced severe changes in temperature and precipitation in the past millions of years, adapting well to harsh environments. In view of global climate change and its endangered conditions, it is crucial to study how it responds to changes in temperature and precipitation for its conservation work. In this study, single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing were combined to generate the complete transcriptome of C. oliveri. Using the RNA-seq data to correct the SMRT sequencing data, the four tissues obtained 63,831 (root), 58,108 (stem), 33,013 (leaf) and 62,436 (male cone) full-length unigenes, with a N50 length of 2523, 3480, 3181, and 3267 bp, respectively. Additionally, 35,887, 11,306, 36,422, and 25,439 SSRs were detected for the male cone, leaf, root, and stem, respectively. The number of long non-coding RNAs predicted from the root was the largest (11,113), and the other tissues were 3408 (stem), 3193 (leaf), and 3107 (male cone), respectively. Functional annotation and enrichment analysis of tissue-specific expressed genes revealed the special roles in response to environmental stress and adaptability in the different four tissues. We also characterized the gene families and pathways related to abiotic factors. This work provides a comprehensive transcriptome resource for C. oliveri, and this resource will facilitate further studies on the functional genomics and adaptive evolution of C. oliveri.
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Komodromos, Dimitrios, Charalampos Kotzamanidis, Virginia Giantzi, Styliani Pappa, Anna Papa, Antonios Zdragas, Apostolos Angelidis, and Daniel Sergelidis. "Prevalence, Infectious Characteristics and Genetic Diversity of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) in Two Raw-Meat Processing Establishments in Northern Greece." Pathogens 11, no. 11 (November 17, 2022): 1370. http://dx.doi.org/10.3390/pathogens11111370.
Повний текст джерелаАнотація:
In the present study, we investigated the isolation frequency, the genetic diversity, and the infectious characteristics of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from the incoming meat and the meat products, the environment, and the workers’ nasal cavities, in two meat-processing establishments in northern Greece. The isolated S. aureus strains were examined for their resistance to antimicrobials, carriage of the mecA and mecC genes, carriage of genes encoding for the production of nine staphylococcal enterotoxins, carriage of the Panton–Valentine Leukocidin and Toxic Shock Syndrome genes, and the ability to form biofilm. The genetic diversity of the isolates was evaluated using Pulsed Field Gel Electrophoresis (PFGE) and spa typing. S. aureus was isolated from 13.8% of the 160 samples examined, while only one sample (0.6%) was contaminated by MRSA carrying the mecA gene. The evaluation of the antimicrobial susceptibility of the isolates revealed low antimicrobial resistance. The higher resistance frequencies were observed for penicillin (68.2%), amoxicillin/clavulanic acid (36.4%) and tetracycline (18.2%), while 31.8% of the isolates were sensitive to all antimicrobials examined. Multidrug resistance was observed in two isolates. None of the isolates carried the mecC or lukF-PV genes, and two isolates (9.1%) harbored the tst gene. Eight isolates (36.4%) carried the seb gene, one carried the sed gene, two (9.1%) carried both the sed and sei genes, and one isolate (4.5%) carried the seb, sed and sei genes. Twenty-one (95.5%) of the isolates showed moderate biofilm production ability, while only one (4.5%) was characterized as a strong biofilm producer. Genotyping of the isolates by PFGE indicates that S. aureus from different meat-processing establishments represent separate genetic populations. Ten different spa types were identified, while no common spa type isolates were detected within the two plants. Overall, our findings emphasize the need for the strict application of good hygienic practices at the plant level to control the spread of S. aureus and MRSA to the community through the end products.
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Naveenkumar, T., M. Baskar, and K. Aswitha. "Kinetics of nitrogen in sodic soil of Kumulur village, Trichy District, Tamil Nadu under different organic and inorganic amendments." Journal of Applied and Natural Science 14, SI (July 15, 2022): 85–89. http://dx.doi.org/10.31018/jans.v14isi.3572.
Повний текст джерелаАнотація:
Currently more than 20 per cent of the world’s irrigated land is salt affected. Of that about 60 per cent are sodic soils, warranting greater attention for efficient and eco-friendly environmentally amelioration techniques. Transformation and availability of several plant nutrient elements are affected by soil sodicity. Alkali/sodic soils are to be reclaimed so as tomake nutrients available to plants optimally. A laboratory incubation study was examined to analyse the impact of various amendments, either alone or in combination with nitrogen (N) @75 kg/ha, on the physico-chemical characteristics and nitrogen dynamics in sodic soil of Kumulur village Trichy District, Tamil Nadu (pH-10.4, EC-0.40 dS, m-1, ESP-31.8). The investigation was conducted at ADAC&RI in Trichy with three replications and eight treatments in a completely randomized design. The treatments used for sodic soil reclamation utilizing a standardized procedure were Gypsum (GYP) + Green manure (GM) @ 6.5 t ha-1 (T2), Distillery spent wash (DSW) @ 5 lakhs liter-1(T4), and Green leaf manure (GLM) @ 12.5 t ha-1(T3). Soil samples were taken at 15-day intervals from the 15th to the 60th day and tested for NH4-N, NO3-N, and accessible nitrogen. Using DSW, GYP + GM, and GLM, the pH of the water was decreased from 10.2 to 8.37, 8.42, and 9.21, respectively. The soil pH dropped the most in the DSW-controlled treatments. The application of nitrogen alone without any amendments (T5) recorded a higher value (135 kg ha-1) during an initial period (15 days) only and thereafter declined sharply due to various losses, i.e., volatilization, denitrification or fixation. When nitrogen was applied along with amendments, a significant (CD-0.05%) buildup in available N contents was observed over the application of N alone. Available nitrogen (325 kg ha-1), nitrate nitrogen (102 kg ha-1) and ammonical nitrogen (205 kg ha-1) were significantly increased due to the addition of amendments. However, the decline in available N with incubation period was only marginal when nitrogen was applied along with amendments. An increase in NH4-N at 30 DAI might be due to the release of nutrients. A slight increase in the nitrate-N content of the soil was observed at the end of the incubation period due to microbial oxidation of NH4-N to NO3-N. The application of amendments could save a quantity of N dose besides reclaiming the sodicity.
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BUKHORI, ALPINA, DWI SURYANTO, and KIKI NURTJAHJA. "Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L." Biodiversitas Journal of Biological Diversity 23, no. 6 (June 6, 2022). http://dx.doi.org/10.13057/biodiv/d230638.
Повний текст джерелаАнотація:
Abstract. Bukhori A, Suryanto D, Nurtjahja K. 2022. Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L. Biodiversitas 23: 3108-3114. Biosurfactants are surface-active molecules produced by living organisms predominantly by microorganisms with amphiphilic properties. Exploration of biosurfactant-producing bacteria has been promoted to find the suitable agent for mass production in the laboratory following its biochemical and genetic modification. Leaf-colonizing bacteria or phyllosphere bacteria are of great interest, including those colonizing the surface of ornamental plants which are still understudied. This study aimed to isolate the biosurfactant-producing bacteria from an ornamental plant, Colocasia esculenta L and to determine their physical characteristics. Four bacterial isolates coded as IC1, IC3, IC4 and IC5 were recovered from the surface of C. esculenta and were tested positive for their growth under Bushnell-Haas agar + 1% olive oil (v/v) as the sole carbon source. Two isolates, namely IC3 and IC5, later molecularly identified as Bacillus cereus and Alcaligenes faecalis produced the highest biosurfactant concentration (IC3 = 157 ppm, IC5 = 106 ppm) on 10th day incubation based on a colorimetric test using rhamnolipid as a standard solution. Crude biosurfactants produced by A. faecalis IC5 showed better physical attributes than B. cereus IC3 in terms of surface tension, emulsification index, and oil spreading capability on four different hydrophobic compounds i.e., kerosene, solar fuel, octane fuel (Pertalite, Pertamax). The results of this study confirmed the existence of biosurfactant-producing bacteria in C. esculenta and the possibility of developing prominent strains for the treatment of hydrocarbon pollution in the environment.
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Fatimah, Siti, ARIFFIN ARIFFIN, ARDIARINI NOER RAHMI, and KUSWANTO KUSWANTO. "Tolerance and determinants of drought character descriptors of the Madurese landrace bambara groundnut (Vigna subterranea)." Biodiversitas Journal of Biological Diversity 21, no. 7 (June 18, 2020). http://dx.doi.org/10.13057/biodiv/d210731.
Повний текст джерелаАнотація:
Abstract. Fatimah S, Ariffin, Rahmi AN, Kuswanto. 2020. Tolerance and determinants of drought character descriptors of the Madurese landrace bambara groundnut (Vigna subterranea). Biodiversitas 21: 3108-3116. Bambara groundnut (Vigna subterranea L. Verdc.) is legume of African origin overlooked in Indonesia. It has underdeveloped in Indonesia; for example, in East Java, it is only cultivated in Gresik, Lamongan, and Bangkalan of Madura. This plant can potentially be developed in dry lands, such as Madura as it has the ability to grow and develop well in a dry environment with low nutrient level. At present, there are sparse researches on the selection and determination of the drought-tolerant character descriptors of bambara groundnut in Indonesia. The present study used the expected lines of bambara groundnut selected from local lines of various regions in Indonesia using the nested design. The results showed that the drought stress treatment led to stunted growth of 12 bambara groundnut genotypes, including the number of leaves, plant height, canopy diameter, leaf thickness, number of flowers, number of stems or branches, number of internodes, length of leaf stems, root length, root wet weight, canopy wet weight, canopy dry weight, root dry weight, and leaf chlorophyll content. However, drought leads to a slight increase in the width and length of the stomata opening and leaf proline contents. The cluster analysis based on stress index and sensitivity index can classify 3 expected lines originating from Gresik Regency (G1, G2, G3), falling into the drought stress-tolerant category. Accumulated proline contents cannot be used as a descriptor of tolerance to drought stresses in bambara groundnut since the expected lines with an increase in proline contents in leaves during drought stresses based on the cluster analysis do not fall into the genotypic cluster tolerant to drought stresses.
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Kots, Serhii, Dmytro Kiriziy, Anastasiia Pavlyshche, and Liliia Rybachenko. "PECULIARITIES OF FORMATION AND FUNCTIONING OF THE SOYBEAN–BRADYRHIZOBIUM JAPONICUM SYMBIOTIC APPARATUS IN RELATION TO PHOTOSYNTHETIC ACTIVITY UNDER THE INFLUENCE OF SEED PROTECTANTS." Journal of microbiology, biotechnology and food sciences, January 19, 2022, e3128. http://dx.doi.org/10.55251/jmbfs.3128.
Повний текст джерелаАнотація:
The aim of work was to investigate the influence of seed treatment with two preparations of fungicidal action, differing in the active substances composition, on the formation and functioning of legume-rhizobial symbiosis in interaction with the photosynthetic apparatus of soybean plants. It was established that in plants treated with preparation Fever, the nitrogen-fixing activity (NFA) difference with the control was insignificant, but under the impact of the preparation Standak Top NFA was by 63% less at the stage of three true leaves, by 48% – at the stage of budding, and by 34% – at the stage of budding–flowering. The net photosynthetic rate, calculated per leaf area unit, was lower than control at all investigated stages of soybean plants development in the variants with the seed protectants applying. A fairly close positive correlations (R2 = 0.79) were observed between the average weight of one nodule and its NFA, and between the last and total NFA of the whole plant (R2 = 0.78). Close correlations between plant NFA, chlorophyll content in leaves, and net photosynthetic rate were revealed. It was concluded that seed protectants significantly influenced on nitrogen fixation and photosynthesis. The strength of manifestation, and the sign of this influence in comparison with the relevant parameters of the control plants depended on the preparation, the stage of plant development, as well as on the index (nodules formation, nitrogen-fixing activity, photosynthetic, chlorophyll content, chlorophyll index, biological productivity of soybean plants) under consideration.
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Mahmudi, Mohammad, Evellin Dewi Lusiana, Endang Y. Herawati, and Lukas G. Serihollo. "Environmental factors and seasonal effect on the potential harmful algae presence at Ambon Bay, Indonesia." Biodiversitas Journal of Biological Diversity 21, no. 7 (June 17, 2020). http://dx.doi.org/10.13057/biodiv/d210730.
Повний текст джерелаАнотація:
Abstract. Mahmudi M, Lusiana ED, Herawati EY, Serihollo LG. 2020. Environmental factors and seasonal effects on the potential harmful algae presence at Ambon Bay, Indonesia. Biodiversitas 21: 3101-3107. Marine and coastal areas are susceptive to harmful algae presence which can lead to Harmful Algae Blooms (HABs). Major drivers for this event are climate change, domestic and industrial activities. These alter the environmental condition in marine ecosystems which caused the shift of phytoplankton community. This study aims to analyze the environmental factors affecting the harmful algae occurrence in Ambon Bay as well as the seasonal change during west monsoon and first transition in regards to this issue. There were six environmental variables accounted in this research which measured in situ and ex-situ. The results showed that there were three algae divisions that formed the phytoplankton structure in Ambon Bay, namely Bacillariophyceae, Dinophyceae, and Cyanophyceae. These divisions have been reported as the cause of previous HABs in the area. Furthermore, algae density during first transition season was higher than during west monsoon season, but the composition of phytoplankton community was stable in which dominated by harmful algae such as Chaetoceros, Skeletonema, Nitzschia, Ceratium, Pyrodinium, Dinophysis, Alexandrium, and Trichodesmium. Meanwhile, based on Canonical Correspondence Analysis (CCA), temperature and nutrients were the main factors that highly associated with the presence and abundance of harmful.
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Gupta, Tanya, and Juhi Saxena. "BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES FROM ASPERGILLUS ORYZAE MTCC 3107 AGAINST PLANT PATHOGENIC FUNGI SCLEROTINIA SCLEROTIORUM MTCC 8785." Journal of microbiology, biotechnology and food sciences, November 15, 2022, e9387. http://dx.doi.org/10.55251/jmbfs.9387.
Повний текст джерелаАнотація:
Phytopathogen including Sclerotinia sclerotiorum is a major problem for agricultural crops. Being safe, antifungal, and environment friendly, silver nanoparticles (AgNPs) are the first choices to combat phytopathogens. In view of this, the present study was designed to formulate AgNPs from Aspergillus oryzae MTCC No. 3107. Biosynthesis of AgNPs by A. oryzae was investigated using cell-free filtrates from fungi cultivated in potato dextrose broth (PDB) and amylase production media (APM). Fungal production media harbour inducers which upregulate secretion of specific enzymes. Amylase is known to catalyze the bio-reduction process. The cell- free filtrates containing extracellularly secreted fungal amylases when exposed to the metal salt solution (silver nitrate) at 1mM concentration, silver ions were reduced to zero oxidation state forming stable nano silver. The colour change was observed and the formation of AgNPs was further characterised by UV–vis spectrophotometry by scanning from 300-700 nm wavelength. Transmission electron microscope characterization revealed a size of 40nm. Further, the FTIR analysis identified the key functional groups involved in the stabilization and capping of AgNPs. Moreover, XRD analysis was done to identify the diffraction pattern in AgNPs. Antifungal effect of synthesized AgNPs on phytopathogen S. sclerotiorum MTCC 8785 was studied using variable concentrations of amylase mediated AgNPs. 100 percent inhibition was observed at 100 μg/ml concentration when compared to positive control.
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Fortunato, Ivan, and Raphael Alves Feitosa. "3.100KM ENTRE PRÁTICAS DE EDUCAÇÃO AMBIENTAL DESCOLONIALIZANTES: EXPERIÊNCIAS DIDÁTICAS NA FORMAÇÃO INICIAL DE PROFESSORES." Educere et Educare 13, no. 29 (November 29, 2018). http://dx.doi.org/10.17648/educare.v13i29.15665.
Повний текст джерелаАнотація:
Este artigo é constituído pela análise de dois relatos de experiência com educação ambiental descolonializante, sendo um desenvolvido no curso de licenciatura em física no Instituto Federal, câmpus de Itapetininga, interior de São Paulo, e o outro no curso de licenciatura em biologia, da Universidade Federal do Ceará. Separados geograficamente por cerca de 3100 quilômetros, o objetivo é o de colocar nossas práticas ambientalmente educativas em evidência e no mesmo plano de equivalência para que, na reflexão interdisciplinar, possamos cooperativamente aprender e ensinar formas mais longevas de educação ambiental.
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FADHIL, WASAN F., ABDULLAH H. AL-SAADOON, and FALEH M. AL-MOUSSAWI. "New records of mycobiota associated with stored wheat and its by-products in Iraq." Biodiversitas Journal of Biological Diversity 23, no. 6 (June 5, 2022). http://dx.doi.org/10.13057/biodiv/d230637.
Повний текст джерелаАнотація:
Abstract. Fadhil WF, Al-Saadoon AH, Al-Moussawi FM. 2022. New records of mycobiota associated with stored wheat and its by-products in Iraq. Biodiversitas 23: 3099-3107. Wheat is a staple food for the Iraqi population and is an important feed source due to its high nutritional value. Consequently, wheat and wheat by-products are susceptible to various species of fungi during processing, transportation, or storage. In this paper, thirty-two selected fungal isolates were subjected to morphological and molecular analysis for identification through combined sequences of ITS regions, actin, calmodulin, glyceraldehyde-3-phosphate dehydrogenase, and ?-tubulin genes according to the target fungal genus. The results showed that eighteen fungal species were identified from wheat grains, flour, and bran samples collected from different silos and mills in three provinces in southern Iraq. In addition, nine species were recorded for the first time from Iraq, i.e., Alternaria consortialis, A. japonica, A. lolii, A. multiformis, A. ventricosa, Aspergillus montevidensis, Cladosporium halotolerans, C. versiforme, and Staganosporopsis tanaceti. Brief descriptions of the new records are presented. This study represents an important addition to the mycobiota of Iraq.
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Thompson, Nathan E., Danielle Rubinstein, William Parrella-O'Donnell, Matthew A. Brett, Brigitte Demes, Susan G. Larson, and Matthew C. O'Neill. "The loss of the ‘pelvic step’ in human evolution." Journal of Experimental Biology 224, no. 16 (August 15, 2021). http://dx.doi.org/10.1242/jeb.240440.
Повний текст джерелаАнотація:
ABSTRACT Human bipedalism entails relatively short strides compared with facultatively bipedal primates. Unique non-sagittal-plane motions associated with bipedalism may account for part of this discrepancy. Pelvic rotation anteriorly translates the hip, contributing to bipedal stride length (i.e. the ‘pelvic step’). Facultative bipedalism in non-human primates entails much larger pelvic rotation than in humans, suggesting that a larger pelvic step may contribute to their relatively longer strides. We collected data on the pelvic step in bipedal chimpanzees and over a wide speed range of human walking. At matched dimensionless speeds, humans have 26.7% shorter dimensionless strides, and a pelvic step 5.4 times smaller than bipedal chimpanzees. Differences in pelvic rotation explain 31.8% of the difference in dimensionless stride length between the two species. We suggest that relative stride lengths and the pelvic step have been significantly reduced throughout the course of hominin evolution.
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Nakamura, Ryotaro, Wael Saber, Michael J. Martens, Alyssa Ramirez, Bart Scott, Betul Oran, Eric Leifer, et al. "Biologic Assignment Trial of Reduced-Intensity Hematopoietic Cell Transplantation Based on Donor Availability in Patients 50-75 Years of Age With Advanced Myelodysplastic Syndrome." Journal of Clinical Oncology, June 9, 2021, JCO.20.03380. http://dx.doi.org/10.1200/jco.20.03380.
Повний текст джерелаАнотація:
PURPOSE Allogeneic hematopoietic cell transplantation (HCT) is the only potentially curative therapy for myelodysplastic syndromes (MDS), although it is infrequently offered to older patients. The relative benefits of HCT over non-HCT therapy in older patients with higher-risk MDS have not been defined. METHODS We conducted a multicenter biologic assignment trial comparing reduced-intensity HCT to hypomethylating therapy or best supportive care in subjects 50-75 years of age with intermediate-2 or high-risk de novo MDS. The primary outcome was overall survival probability at 3 years. Between January 2014 and November 2018, we enrolled 384 subjects at 34 centers. Subjects were assigned to the Donor or No-Donor arms according to the availability of a matched donor within 90 days of study registration. RESULTS The median follow-up time for surviving subjects was 34.2 months (range: 2.3-38 months) in the Donor arm and 26.9 months (range: 2.4-37.2 months) in the No-Donor arm. In an intention-to-treat analysis, the adjusted overall survival rate at 3 years in the Donor arm was 47.9% (95% CI, 41.3 to 54.1) compared with 26.6% (95% CI, 18.4 to 35.6) in the No-Donor arm ( P = .0001) with an absolute difference of 21.3% (95% CI, 10.2 to 31.8). Leukemia-free survival at 3 years was greater in the Donor arm (35.8%; 95% CI, 29.8 to 41.8) compared with the No-Donor arm (20.6%; 95% CI, 13.3 to 29.1; P = .003). The survival benefit was seen across all subgroups examined. CONCLUSION We observed a significant survival advantage in older subjects with higher-risk MDS who have a matched donor identified and underwent reduced-intensity HCT, when compared with those without a donor. HCT should be included as an integral part of MDS management plans in fit older adults with higher-risk MDS.
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SETIAWAN, EDWIN, MOHAMMAD RIZKI CHODIANTORO, GHULAM FATHUR INSANY, IWENDA BELA SUBAGIO, NURUL KUSUMA DEWI, and FARID KAMAL MUZAKI. "Short Communication: Diversity of sponges associated in seagrass meadows at coastal area of Pacitan District, East Java, Indonesia." Biodiversitas Journal of Biological Diversity 22, no. 8 (July 8, 2021). http://dx.doi.org/10.13057/biodiv/d220803.
Повний текст джерелаАнотація:
Abstract. Setiawan E, Chodiantoro MR, Insany GF, Subagio IB, Dewi NK, Muzaki FK. 2021. Short Communication: Diversity of sponges associated in seagrass meadows at coastal area of Pacitan District, East Java, Indonesia. Biodiversitas 22: 3105-3112. Sponges are sessile organisms and aquatic filter feeders that play an important role in marine ecosystems. They are also secondary metabolite compounds that exhibit promising novel properties. Indonesia has been categorized as a hot spot for sponge diversity. Despite this fact, some Indonesian coastal areas, especially in the southern area of East Java, have not yet been comprehensively explored to understand its diversity and potential value. For this reason, we investigated species richness of sponges associated with seagrass meadows in the southern coastal area of Pacitan District, in East Java. Seagrass meadows are important coastal areas that have been recognized as buffer zones for sustainable marine conservation because it provides safe shelter and acts as a nursery for many fishes and other invertebrates, including sponges. We identified 16 genera out of 12 families from two selected beaches in Pacitan. Boring sponges belonging to the Clionaidea family were dominant in those areas, covering more than 20 percent of the total recorded specimens. Moreover, conspicuous boring sponges in this study can be used for further environment alert since this group plays a vital role and negatively impacts destroying the calcium carbonate of marine organisms.
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Rakhmawati, Anna, Endang Tri Wahyuni, and Triwibowo Yuwono. "Thermophilic bacteria isolated from Mount Merapi, Java, Indonesia as a potential lead bioremediation agent." Biodiversitas Journal of Biological Diversity 22, no. 6 (May 9, 2021). http://dx.doi.org/10.13057/biodiv/d220612.
Повний текст джерелаАнотація:
Abstract. Rakhmawati A, Wahyuni ET, Yuwono T. 2021. Thermophilic bacteria isolated from Mount Merapi, Java, Indonesia as a potential lead bioremediation agent. Biodiversitas 22: 3101-3110. Contamination by lead (Pb) has become a serious health and environmental problem, that has to be urgently prevented. Bioremediation is one of the environmentally friendly methods for eliminating Pb from the contaminated environment. This study was aimed to investigate the potency of lead-tolerant thermophilic bacteria isolated from solfatara Mount Merapi, Indonesia. A total of 340 isolates of thermophilic bacteria were screened for lead tolerance at 55 °C. Five bacterial isolates were found to show tolerance to 100 mg/L Pb (II), and then were further evaluated and identified as Aeribacillus pallidus strains MRP112, MRP148, MRP272, MRP278, and MRP280 based on 16S rRNA gene sequences analysis. Among the five isolates, A. pallidus MRP 280 showed highest activity in removing Pb at pH 6, 55°C for 24 h. The analysis of scanning and transmission electron microscopy, biofilm formation, and siderophore production, demonstrated that lead tolerance of A. pallidus MRP 280 strain was also accompanied by morphological changes, bioaccumulation, biosorption, biofilm, and siderophore assembly. In conclusion A. pallidus MRP 280 was demonstrated as one of the most potential bacterial strains, can be recommended as an agent for high-temperature lead bioremediation.
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Nasri, Nasri, Urip Harahap, Jansen Silalahi, and Denny Satria. "Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria." Biodiversitas Journal of Biological Diversity 22, no. 8 (July 8, 2021). http://dx.doi.org/10.13057/biodiv/d220802.
Повний текст джерелаАнотація:
Abstract. Nasri, Harahap U, Silalahi J, Satria D. 2021. Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria. Biodiversitas 22: 3098-3104. Diarrhea is the discharge of liquid or watery stools 3 to 4 times a day caused by a bacterial infection. Treatments for diarrhea are probiotics, which have a beneficial effect on the health of the host such as antibacterial. Traditional Batak Toba fermented food, Dengke Naniura, is a source of probiotics. This study aimed to determine the minimum inhibitory concentration, minimum bactericidal concentration, and leakage of DNA and protein from lactic acid bacteria against pathogens. Isolation of LAB was obtained from Dengke Naniura by pour plate method on deMann Rogosa and Sharpe Agar + CaCO3 1%. In this study, Characterization and analysis of bacterial sequencing used Polymerase Chain Reaction. Determination of MIC used the agar diffusion method. The MBC test used the streaking method which was a stroke from the inhibition zone formed. DNA and protein leakage was measured using spectrophotometry UV-VIS (260nm and 280nm). The isolation results obtained were Lactobacillus fermentum, the characterization showed that the bacteria were Gram-positive, bacilli, non-sporing, catalase-negative, and able to ferment sugar. The MIC determination was obtained at a concentration of 10%v/v with a clear zone diameter. Determination of MBC against pathogens was obtained at different concentrations. The results of DNA and protein leakage showed an increased absorption (260nm and 280nm).
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Ngcaba, Pelisa, and Alfred Maroyi. "Home gardens in the Eastern Cape Province, South Africa: A promising approach to enhance household food security and well-being." Biodiversitas Journal of Biological Diversity 22, no. 9 (September 8, 2021). http://dx.doi.org/10.13057/biodiv/d220953.
Повний текст джерелаАнотація:
Abstract. Ngcaba P, Maroyi A. 2021. Home gardens in the Eastern Cape Province, South Africa: A promising approach to enhance household food security and well-being. Biodiversitas 22: 4045-4053. The current study is aimed at documenting the diversity and composition of food plants cultivated and maintained in home gardens; and also assessing their role at enhancing household food security and well-being in the Eastern Cape province, South Africa. This study was undertaken in six villages in the province, between June 2014 and March 2016. Information on socioeconomic and demographic characteristics of the respondents, home garden practices, food plants grown and maintained in home gardens were collected by means of questionnaires and guided field walks with 129 respondents. A total of 32 edible plants belonging to 27 genera and 18 families were recorded. The main uses of plants grown and managed in home gardens were edible fruits or seeds (56.3%), leaves cooked as leafy vegetables (28.1%), edible bulbs, roots and tubers (21.9%), beverage, cereal, and culinary herb or spice (3.1% each). The most preferred species were Zea mays (64.3%), followed by Brassica oleracea (59.7%), Solanum tuberosum (58.9%), Spinacia oleracea (52.7%), Allium cepa (51.9%), Cucurbita moschata (44.2%), Capsicum annuum (38.0%), Cucurbita maxima (31.8%) and Daucas carota (31.0%). This study showed that home gardens can provide alternative sources of diverse and nutritious food to households, making home gardens an important agricultural resource for increasing food security in the province.
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ISRONI, WAHYU, R. ADHARYAN ISLAMY, MOHAMAD MUSA, and PUTUT WIJANARKO. "Short Communication: Species composition and density of mangrove forest in Kedawang Village, Pasuruan, East Java, Indonesia." Biodiversitas Journal of Biological Diversity 20, no. 6 (May 24, 2019). http://dx.doi.org/10.13057/biodiv/d200626.
Повний текст джерелаАнотація:
Abstract. Isroni W, Islamy RA, Musa M, Wijanarko P. 2019. Short Communication: Species composition and density of mangrove forest in Kedawang Village, Pasuruan, East Java. Biodiversitas 20: 1688-1692. Mangrove is one of ecosystems located in estuary and shallow coastal waters and its existence has a significant role for life. The aim of this study was to identify species composition and density of mangrove forest in Kedawang village, Nguling Sub-district, Pasuruan District, East Java, Indonesia. Survey method using belt transects was carried at three sampling points. Results of this study showed that mangrove vegetation in Kedawang includes five species from three families, namely Avicenniaceae (Avicennia alba and Avicennia marina), Rhizophoraceae (Rhizophora apiculata and Rhizophora mucronata), and Sonneratiace (Sonneratia alba). At sampling point 1, A. alba dominated with a density of 37 individuals/ha at tree level, 380 individuals/ha at pole level and 3100 individuals/ha at stake level. At sampling point 2, the tree level was dominated by A. alba with 142 individuals/ha, while R. mucronata dominated the pole and stake level with 1300 and 1467individuals/ha, respectively. At sampling point 3, the pole and stake level was dominated by A. alba with 167 and 933 individuals/ha respectively, while A. marina dominated the sapling level with 800 individuals/ha. At all stations, the density at stake level is greater than that tree level. Therefore, it is necessary to carry out conservation efforts in the form of protection combined with restoration by enrichment planting at all study locations to increase the diversity and density of mangrove vegetation.
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Berame, Julie, N. LAWSIN, F. MIGUEL, and J. CHAVEZ. "Morphological variations of mangosteen fruits from Luzon and Mindanao Islands, the Philippines." Biodiversitas Journal of Biological Diversity 21, no. 7 (June 17, 2020). http://dx.doi.org/10.13057/biodiv/d210729.
Повний текст джерелаАнотація:
Abstract. Berame J, Lawsin N, Miguel F, Chavez J. 2020. Morphological variations of mangosteen fruits from Luzon and Mindanao Islands, the Philippines. Biodiversitas 21: 3094-3100. Luzon and Mindanao islands in the Philippines are the important mangosteen producing provinces whose growing areas are scattered on these two islands. With this, mangosteen variability was detected based on field fruit collections in summer of 2019. The morphological variations of mangosteen fruits were observed and collected in Quezon Province in Luzon and Davao-Zamboanga Provinces in Mindanao based on the total number of 175 mangosteen trees. The main objectives of the research were to find out the morphological variations of mangosteen fruits and using Tomato Analyzer 4.0 to determine the morphological characters that can be used to differentiate mangosteen accessions in the Philippines. The variables include fruit shape index (external and internal), curve fruit shape index, asymmetry, internal eccentricity and weight of the ripe fruits. The results show that fruit characters like ellipsoidal, circular, rectangular, lobe shape, size, pericarp area, and pericarp thickness. are highly resemblance particularly in its fruit shape characters, and even sharing a similar taste of ripe fruits. Data revealed that Garcinia mangostana shared a similar character with Garcinia malaccensis even though they are from different geographical areas. Based on the morphological variations and characters, the two varieties of mangosteen fruits in the Philippines, G. mangostana and G. malaccensis, are so closely related that they could be combined together as one species as identified in the morphological analysis of this study.
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Cait, Jessica, Alissa Cait, R. Wilder Scott, Charlotte B. Winder, and Georgia J. Mason. "Conventional laboratory housing increases morbidity and mortality in research rodents: results of a meta-analysis." BMC Biology 20, no. 1 (January 13, 2022). http://dx.doi.org/10.1186/s12915-021-01184-0.
Повний текст джерелаАнотація:
Abstract Background Over 120 million mice and rats are used annually in research, conventionally housed in shoebox-sized cages that restrict natural behaviours (e.g. nesting and burrowing). This can reduce physical fitness, impair thermoregulation and reduce welfare (e.g. inducing abnormal stereotypic behaviours). In humans, chronic stress has biological costs, increasing disease risks and potentially shortening life. Using a pre-registered protocol (https://atrium.lib.uoguelph.ca/xmlui/handle/10214/17955), this meta-analysis therefore tested the hypothesis that, compared to rodents in ‘enriched’ housing that better meets their needs, conventional housing increases stress-related morbidity and all-cause mortality. Results Comprehensive searches (via Ovid, CABI, Web of Science, Proquest and SCOPUS on May 24 2020) yielded 10,094 publications. Screening for inclusion criteria (published in English, using mice or rats and providing ‘enrichments’ in long-term housing) yielded 214 studies (within 165 articles, using 6495 animals: 59.1% mice; 68.2% male; 31.8% isolation-housed), and data on all-cause mortality plus five experimentally induced stress-sensitive diseases: anxiety, cancer, cardiovascular disease, depression and stroke. The Systematic Review Center for Laboratory animal Experimentation (SYRCLE) tool assessed individual studies’ risks of bias. Random-effects meta-analyses supported the hypothesis: conventional housing significantly exacerbated disease severity with medium to large effect sizes: cancer (SMD = 0.71, 95% CI = 0.54–0.88); cardiovascular disease (SMD = 0.72, 95% CI = 0.35–1.09); stroke (SMD = 0.87, 95% CI = 0.59–1.15); signs of anxiety (SMD = 0.91, 95% CI = 0.56–1.25); signs of depression (SMD = 1.24, 95% CI = 0.98–1.49). It also increased mortality rates (hazard ratio = 1.48, 95% CI = 1.25–1.74; relative median survival = 0.91, 95% CI = 0.89–0.94). Meta-regressions indicated that such housing effects were ubiquitous across species and sexes, but could not identify the most impactful improvements to conventional housing. Data variability (assessed via coefficient of variation) was also not increased by ‘enriched’ housing. Conclusions Conventional housing appears sufficiently distressing to compromise rodent health, raising ethical concerns. Results also add to previous work to show that research rodents are typically CRAMPED (cold, rotund, abnormal, male-biased, poorly surviving, enclosed and distressed), raising questions about the validity and generalisability of the data they generate. This research was funded by NSERC, Canada.