Готові списки джерел за темами / ATCC 53103

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Добірка наукової літератури з теми "ATCC 53103"

Автор: Grafiati

Опубліковано: 14 березня 2023

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Статті в журналах з теми "ATCC 53103"

SALMINEN, SEPPO J., and D. C. DONOHUE. "Safety Assessment of Lactobacillus Strain GG (ATCC 53103)." Nutrition Today 31, Supplement 1 (November 1996): 16S. http://dx.doi.org/10.1097/00017285-199611001-00005.

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Fan, Xuejing, Kenan Zhang, Zongcai Zhang, Zhen Zhang, Xue Lin, Xin Liu, Zhen Feng, and Huaxi Yi. "Ribosome Profiling Reveals Genome-Wide Cellular Translational Regulation in Lacticaseibacillus rhamnosus ATCC 53103 under Acid Stress." Foods 11, no. 10 (May 13, 2022): 1411. http://dx.doi.org/10.3390/foods11101411.

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During fermentation and food processing, Lacticaseibacillus rhamnosus ATCC 53103 can encounter many adverse conditions, and acid stress is one of them. The purpose of the present study was to investigate the influence of acid stress on the global translational and transcriptional regulation of Lacticaseibacillus rhamnosus ATCC 53103. Two pH values (pH 6.0 vs. pH 5.0) were applied, the effects of which were studied via ribosome profiling and RNA sequencing assay. Under acid stress, many genes showed differential changes at the translational and transcriptional levels. A total of 10 genes showed different expression trends at the two levels. The expression of 337 genes—which mainly participated in the ABC transporters, amino acid metabolism, and ribosome functional group assembly pathways—was shown to be regulated only at the translational level. The translational efficiency of a few genes participating in the pyrimidine and amino acid metabolism pathways were upregulated. Ribosome occupancy data suggested that ribosomes accumulated remarkably in the elongation region of open reading frame regions under acid stress. This study provides new insights into Lacticaseibacillus rhamnosus ATCC 53103 gene expression under acid stress, and demonstrates that the bacterium can respond to acid stress with synergistic translational and transcriptional regulation mechanisms, improving the vitality of cells.

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Savino, Francesco, Paola Montanari, Ilaria Galliano, Valentina Daprà, and Massimiliano Bergallo. "Lactobacillus rhamnosus GG (ATCC 53103) for the Management of Infantile Colic: A Randomized Controlled Trial." Nutrients 12, no. 6 (June 5, 2020): 1693. http://dx.doi.org/10.3390/nu12061693.

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Background: The aim of this study was to investigate the efficacy of Lactobacillus rhamnosus ATCC 53103 together with the maternal diet avoidance of cow’s milk in treating infantile colic. Methods: Forty-five colicky breastfed infants were consecutively randomized to receive L. rhamnosus for 28 days at a dosage of 5 × 109 cfu per day or placebo. Faecal samples were collected from each subject before starting supplementation and at the end of the study period and were immediately analysed. Faecal calprotectin was detected via a quantitative assay. The total bacterial load and selected bacterial species were evaluated using real-time TaqMan PCR. Results: After supplementation for 28 days with Lactobacillus rhamnosus ATCC 53103, median full-force daily crying was reduced (104 versus 242 min, p < 0.001) and the values of faecal calprotectin decreased significantly (p = 0.026). Furthermore, the probiotic increased the abundance of Lactobacillus (p = 0.048) and total bacteria (p = 0.040); all these effects were not observed in the placebo group. Conclusion: Infants treated with Lactobacillus rhamnosus ATCC 53103 for 28 days, in association with the elimination of cow’s milk from the maternal diet, presented some interesting features related to the effect of this probiotic treatment: reductions in crying time and faecal calprotectin, with increased total bacteria and Lactobacillus. To validate these results, a double-blind, placebo-controlled trial on a larger cohort is required.

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Asahara, Takashi, Masatoshi Takahashi, Koji Nomoto, Hiroo Takayama, Masaharu Onoue, Masami Morotomi, Ryuichiro Tanaka, Teruo Yokokura, and Naoya Yamashita. "Assessment of Safety of Lactobacillus Strains Based on Resistance to Host Innate Defense Mechanisms." Clinical Diagnostic Laboratory Immunology 10, no. 1 (January 2003): 169–73. http://dx.doi.org/10.1128/cdli.10.1.169-173.2003.

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ABSTRACT Seven Lactobacillus strains belonging to four species were evaluated for pathogenicity as well as for in vitro sensitivity to the bactericidal mechanisms of macrophages in a rabbit infective endocarditis (IE) model. Two bacteremia-associated strains, L. rhamnosus PHLS A103/70 and L. casei PHLS A357/84, as well as the L. rhamnosus type strain and the probiotic L. rhamnosus strain ATCC 53103, showed moderate infectivity, and the virulence of the probiotic L. casei strain Shirota and type strains such as L. acidophilus ATCC 4356T and L. gasseri DSM 20243T in the model was negligible. The strains that showed pathogenic potential in the rabbit IE model (PHLS A357/84, PHLS A103/70, and ATCC 53103) were more resistant than strain Shirota to intracellular killing activity by mouse macrophages in vitro and also to bactericidal nitrogen intermediates, such as nitric oxide and NO2 − ions. These results suggest that resistance to host innate defense systems, which would function at inflammatory lesions, should be considered in the safety assessment of Lactobacillus strains.

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Morita, Hidetoshi, Hidehiro Toh, Kenshiro Oshima, Masaru Murakami, Todd D. Taylor, Shizunobu Igimi, and Masahira Hattori. "Complete Genome Sequence of the Probiotic Lactobacillus rhamnosus ATCC 53103." Journal of Bacteriology 191, no. 24 (October 9, 2009): 7630–31. http://dx.doi.org/10.1128/jb.01287-09.

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ABSTRACT Lactobacillus rhamnosus is a facultatively heterofermentative lactic acid bacterium and is frequently isolated from human gastrointestinal mucosa of healthy individuals. L. rhamnosus ATCC 53103, isolated from a healthy human intestinal flora, is one of the most widely used and well-documented probiotics. Here, we report the finished and annotated genome sequence of this organism.

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Landersjö, Clas, Zhennai Yang, Eine Huttunen, and Göran Widmalm. "Structural Studies of the Exopolysaccharide Produced byLactobacillusrhamnosusstrain GG (ATCC 53103)." Biomacromolecules 3, no. 4 (July 2002): 880–84. http://dx.doi.org/10.1021/bm020040q.

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Korpela, Riitta, Eeva Moilanen, Maija Saxelin, and Heikki Vapaatalo. "Lactobacillus rhamnosus GG (ATCC 53103) and platelet aggregation in vitro." International Journal of Food Microbiology 37, no. 1 (June 1997): 83–86. http://dx.doi.org/10.1016/s0168-1605(97)00049-4.

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Nikoskelainen, Sami, Seppo Salminen, Göran Bylund, and Arthur C. Ouwehand. "Characterization of the Properties of Human- and Dairy-Derived Probiotics for Prevention of Infectious Diseases in Fish." Applied and Environmental Microbiology 67, no. 6 (June 1, 2001): 2430–35. http://dx.doi.org/10.1128/aem.67.6.2430-2435.2001.

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ABSTRACT The present study aimed to investigate the potential probiotic properties of six lactic acid bacteria (LAB) intended for human use, Lactobacillus rhamnosus ATCC 53103,Lactobacillus casei Shirota, Lactobacillus bulgaricus, L. rhamnosus LC 705, Bifidobacterium lactis Bb12, and Lactobacillus johnsonii La1, and one for animal use, Enterococcus faecium Tehobak, for use as a fish probiotic. The strains for human use were specifically chosen since they are known to be safe for human use, which is of major importance because the fish are meant for human consumption. The selection was carried out by five different methods: mucosal adhesion, mucosal penetration, inhibition of pathogen growth and adhesion, and resistance to fish bile. The adhesion abilities of the seven LAB and three fish pathogens, Vibrio anguillarum, Aeromonas salmonicida, and Flavobacterium psychrophilum, were determined to mucus from five different sites on the surface or in the gut of rainbow trout. Five of the tested LAB strains showed considerable adhesion to different fish mucus types (14 to 26% of the added bacteria). Despite their adhesive character, the LAB strains were not able to inhibit the mucus binding of A. salmonicida. Coculture experiments showed significant inhibition of growth of A. salmonicida, which was mediated by competition for nutrients rather than secretion of inhibitory substances by the probiotic bacteria as measured in spent culture liquid. All LAB except L. casei Shirota showed tolerance against fish bile. L. rhamnosus ATCC 53103 and L. bulgaricus were found to penetrate fish mucus better than other probiotic bacteria. Based on bile resistance, mucus adhesion, mucus penetration, and suppression of fish pathogen growth,L. rhamnosus ATCC 53103 and L. bulgaricus can be considered for future in vivo challenge studies in fish as a novel and safe treatment in aquaculture.

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Serra, Stefano, Davide De Simeis, Stefano Marzorati, and Mattia Valentino. "Oleate Hydratase from Lactobacillus rhamnosus ATCC 53103: A FADH2-Dependent Enzyme with Remarkable Industrial Potential." Catalysts 11, no. 9 (August 30, 2021): 1051. http://dx.doi.org/10.3390/catal11091051.

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Recently, we described the preparation of the recombinant oleate hydratase from Lactobacillus rhamnosus ATCC 53103. We observed that the purified C-terminal His-tagged enzyme was completely inactive and the catalytic activity was partially restored only in presence of a large amount of flavin adenine dinucleotide (FAD). In the present work, we assess that this hydratase in the presence of the reduced form of flavin adenine dinucleotide (FADH2) is at least one hundred times as active as in the presence of the same concentration of FAD. By means of two different biochemical processes, we demonstrated unambiguously that oleate hydratase from Lactobacillus rhamnosus ATCC 53103 is a FADH2-dependent enzyme. As a first relevant application of this discovery, we devised a preparative procedure for the stereoselective synthesis of (R)-10-hydroxystearic acid. Accordingly, the hydration of oleic acid (up to 50 g/L) is performed on a multigram scale using the recombinant hydratase and FADH2 generated in situ as cofactor. The produced (R)-10-hydroxystearic acid (ee > 97%) precipitates from the reaction solvent (water/glycerol/ethanol) and is conveniently recovered by simple filtration (>90% yield).

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Drago, L., V. Rodighiero, R. Mattina, M. Toscano, and E. De Vecchi. "In VitroSelection of Antibiotic Resistance in the Probiotic StrainLactobacillus rhamnosusGG ATCC 53103." Journal of Chemotherapy 23, no. 4 (August 2011): 211–15. http://dx.doi.org/10.1179/joc.2011.23.4.211.

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Більше джерел

Дисертації з теми "ATCC 53103"

Iodice, Domenico. "Construction and characterization of probiotic Lactobacillus rhamnosus GG deletion mutant for extracellular polysaccharide (EPS) biosynthesis regulator." Doctoral thesis, Università di Siena, 2018. http://hdl.handle.net/11365/1037713.

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Lactobacillus rhamnosus GG ATCC 53103 (LGG) is a probiotic microorganism producing a galactose-rich exopolysaccharide (EPS) surrounding the cell wall involved in adhesion and biofilm formation. The EPS gene cluster is composed by 16 genes associated to EPS biosynthesis. The wzb gene is an ortholog of the Streptococcus pneumoniae cpsB, encoding a protein phosphatase involved in the regulation of capsule production. In order to study the role of wzb gene in EPS biosynthesis, a L. rhamnosus GG wzb deletion mutant was constructed. Deletion of wzb produced a 12 minutes reduction of duplication time during growth. Deletion mutant strain produced smaller colonies than wild-type strain. Alcian Blue and India Ink staining denoted a strong reduction of the halo surrounding cells likely associated to EPS in Δwzb strain. Zeta potential analysis showed 2 bacterial populations in the Δwzb strain, while a single population was present in wild type indicating different Zeta potential values and consequently different electrokinetic properties of bacterial surfaces in the isogenic strains. Flow cytometry and dot blot analyses were used to assess the binding of Wheat Germ Agglutinin (WGA) and Chum Salmon Lectin 3 (CSL-3) lectins to the Nacetylglucosamine and rhamnose residues of EPS. In presence of WGA, 2 bacterial populations were observed in Δwzb strain, while a single population in wild type strain. In Δwzb strain a strong reduction of CSL-3 binding was observed. Furthermore, deletion of wzb is responsible of a significant reduction in biofilm production as estimated by crystal violet coloration. A general reduction of gene expression level of the EPS gene cluster was observed in Δwzb strain from the mid exponential to the early stationary growth phases. The present data reported the first inactivation of wzb gene in L. rhamnosus and the functional characterization of the Δwzb strain confirmed a role of Wzb phosphatase in EPS production.

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Ananta, Edwin [Verfasser]. "Impact of environmental factors on viability and stability and high pressure pretreatment on stress tolerance of Lactobacillus rhamnosus GG (ATCC 53103) during spray drying / vorgelegt von Edwin Ananta." 2005. http://d-nb.info/976924277/34.

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