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Дисертації з теми "Bacterial genetics"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 25 липня 2025

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1

Martins, Thaísa Zanetoni. "Mutagênese sítio-dirigida da ORF XAC0024 de Xanthomonas citri subsp. citri e suas implicações no desenvolvimento do cancro cítrico /." Jaboticabal, 2016. http://hdl.handle.net/11449/138238.

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Orientador: Jesus Aparecido Ferro<br>Coorientador: Helen Alves Penha<br>Banca: Fabrício José Jaciani<br>Banca: Flávia Maria de Souza Carvalho<br>Resumo: O cancro cítrico tem como agente causal a bactéria Xanthomonas citri subsp. citri (Xac), que afeta diferentes espécies de citros economicamente importantes. É uma doença ainda sem método curativo, e pela sua relevância e dano econômico, faz-se necessário o entendimento em termos moleculares da interação Xac-citros para o desenvolvimento de estratégias que controlem a doença. O objetivo do presente trabalho foi investigar os efeitos da deleção

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2

Cock, J. M. "Bacterial nitrate reductase genes." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355501.

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3

Ennis, Don Gregory. "Genetics of SOS mutagenesis." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184602.

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Previous genetic evidence suggested that RecA was required in SOS mutagenesis for its regulatory role and perhaps some other nonregulatory role (Mount, 1977; Blanco et al., 1982). I undertook a genetic study which confirmed the above studies and provided further evidence that RecA protein appeared to have a dual "role in mutagenesis; first, the cleavage of LexA repressor for the derepression of specific SOS genes and second, one or more additional role(s). For these studies a new phage mutagenesis assay was developed which allows rapid scoring of SOS mutagenesis in a large number of host mutan

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4

Leiman, Sara. "Genetics and Regulation of Bacterial Biofilms." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:17463954.

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Bacterial biofilm formation, the construction of dense, protective, multicellular communities, is a widely conserved behavior. In some bacteria, such as the Gram-positive model organism Bacillus subtilis, the genetics controlling biofilm formation are well understood. In other bacteria, however, including the Gram-negative opportunistic pathogen Pseudomonas aeruginosa, the identities or roles of many biofilm genes remain unknown. Importantly, many proposed applications of biofilm research, particularly in the medical field, require knowledge not only of biofilm assembly but also of biofilm

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5

Parahitiyawa, Nipuna Bandara. "Phylogenetic aspects of oral bacterial microbiome." Thesis, Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43278486.

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6

Rollie, Clare. "Memories are made of this : investigating the CRISPR-Cas adaption mechanism." Thesis, University of St Andrews, 2017. http://hdl.handle.net/10023/10814.

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CRISPR-Cas is an adaptive immune system unique to prokaryotes, which prevents infection by foreign genetic elements. Key to the function of CRISPR-Cas immunity is the ability to adapt to new threats in incorporating short segments, termed spacers, of invading DNA into the clustered regularly interspaced short palindromic repeat (CRISPR) array of the host. Spacers constitute immunological memories, used by CRISPR-associated (Cas) proteins to mount a sequence-specific attack on subsequent infections. The immunisation of the host is called CRISPR adaption. Adaption requires the integration of new

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7

Tsang, J. S. H. "The physiology and genetics of bacterial dehalogenases." Thesis, University of Kent, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380588.

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8

Koh, Cheng Gee. "New approaches for mapping bacterial genomes." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240188.

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9

Cook, Marisa Anne. "Replicons derived from endogenously isolated plasmids used to classify plasmids occurring in marine sediment bacteria." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/25736.

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10

Okuklu, Burcu Güneş Hatice. "Investigation of chromosomal and plasmid dna profiles of lactococcus lactics ssp. lactis/." [s.l.]: [s.n.], 2005. http://library.iyte.edu.tr/tezler/master/biyoloji/T000396.pdf.

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Thesis (Master)--İzmir Institute of Technology, İzmir, 2005<br>Keywords: Lactococcus lactis ssp. lactis, chromosome profiling, pulsed field gel electrophoresis, plasmid profiling, plasmid stability. Includes bibliographical references (leaves 58-63)

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11

Davidson, Seana Kelyn. "Biology of the bryostatins in the marine bryozoan Bugula neritina : symbiosis, cryptic speciation and chemical defense /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1999. http://wwwlib.umi.com/cr/ucsd/fullcit?p3035405.

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12

Hartford, Trudy. "An evaluation of DNA pairing in bacterial systematics." Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/35384.

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The main aim of this work was to evaluate the use of DNA-DNA pairing techniques in bacterial systematics. The genus Listeria was chosen for the study because of the small number of biochemical differences between the seven species. Also there has been a limited amount of nucleic acid studies carried out on the group using an endonuclease technique (Rocourt et al., 1982), therefore some comparisons of the two techniques were possible. Using optical DNA-DNA reassociation on a spectrophotometer with 23 Listeria strains from the seven species, a complete matrix of DNA-DNA homology values was produ

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13

Loman, Nicholas James. "Comparative bacterial genomics." Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/2839/.

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For the most part, diagnostic clinical microbiology still relies on 19th century ideas and techniques, particularly microscopy and laboratory culture. In this thesis I investigate the utility of a new approach, whole-genome sequencing (WGS), to tackle current issues in infectious disease. I present four studies. The first demonstrates the utility of WGS in a hospital outbreak of Acinetobacter baumannii. The second study uses WGS to examine the evolution of drug resistance following antibiotic treatment. I then explore the use of WGS prospectively during an international outbreak of food-borne

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14

余慧儀 and Wai-yee Annie Yu. "Epidemiological and emm gene analysis of non-m-typeable group A streptococcus isolates from Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31969987.

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15

Ramesar, Rajkumar Sewcharan. "Developmental genetic studies on Thiobacillus ferrooxidans." Doctoral thesis, University of Cape Town, 1988. http://hdl.handle.net/11427/26235.

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Thiobacillus ferrooxidans is an industrially important bacterium which is used in bioleaching operations. The work reported in this investigation extends current knowledge of the genetics of this organism. Conjugation was attempted as a means for plasmid DNA transfer to T. ferrooxidans. Recombinant T. ferrooxidans plasmids, pDER401 and pDER405, were shown to code for mobilization and replication functions in Escherichia coli and Thiobacillus novellus strains. The plasmids were mobilizable at high frequency by the IncP plasmid, R68.45. Attempts to transfer the T. ferrooxidans recombinant plasmi

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16

Leung, Kei-chun Jane. "Purification of a transcriptional regulator of the dehalogenase IVa gene of Burkholderia species MBA4." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B38734709.

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17

Leung, Kei-chun Jane, and 梁奇珍. "Purification of a transcriptional regulator of the dehalogenase IVa gene of Burkholderia species MBA4." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B38734709.

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18

Deng, Liyu, and 鄧麗瑜. "Exploration of the transcription factors that regulate the expression of the haloacid operon in Burkholderia caribensis MBA4." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208618.

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Bacterial dehalogenase is a key enzyme involved in bioremediation of halogenated organic compounds. A dehalogenase, Deh4a, was isolated from the Gram-negative bacterium Burkholderia caribensis MBA4, which can utilize haloacetic acids as carbon source. The haloacid operon in MBA4 was identified and characterized. It is composed of the structural genes forDeh4a and a transporter Deh4p. Transcription of this operon is negatively regulated, but the mechanism and the relevant regulator are still poorly understood. In this study, magnetic DNA affinity chromatography and Tn5transposon mutagenesis wer

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19

Osborn, Andrew Mark. "Evolutionary analysis of bacterial mercury resistance in natural environments." Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262489.

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20

Osbourn, Susanne Elizabeth Vana. "The evolution of the Tn21 subgroup of bacterial transposons." Thesis, University of Bristol, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284317.

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21

Scragg, Ian G. "The specificity of intracellular protein degradation in bacterial cells." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/14370.

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22

Marcinkiewicz, Ashley. "Bacterial and phage interactions influencing Vibrio parahaemolyticus ecology." Thesis, University of New Hampshire, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10127507.

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<p> <i>Vibrio parahaemolyticus,</i> a human pathogenic bacterium, is a naturally occurring member of the microbiome of the Eastern oyster. As the nature of this symbiosis in unknown, the oyster presents the opportunity to investigate how microbial communities interact with a host as part of the ecology of an emergent pathogen of importance. To define how members of the oyster bacterial microbiome correlate with <i>V. parahaemolyticus,</i> I performed marker-based metagenetic sequencing analyses to identify and quantify the bacterial community in individual oysters after culturally-quantifying

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23

Papapostolou, Anastasia. "Role of Genetics in Subgingival and Supragingival Bacterial Colonization." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243453546.

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24

Bone, E. J. "Biochemistry and genetics of sporulation in Bacillus subtilis." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377250.

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25

Pinyon, Rebecca A. "Isolation and characterisation of novel non-ribosomal peptide synthetase genes from the entomopathogenic Xenorhabdus bovienii T228." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09php659.pdf.

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26

Pierce, Eric John. "Bacterial toxins as probes for membrane glycolipids in mammalian cells." Thesis, University of Leicester, 1985. http://hdl.handle.net/2381/35129.

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Cholera toxin which binds specifically and with high affinity to a glycolipid; ganglioside GM1, has been used as a probe to study glycolipid-protein interactions in the plasma membranes of BALB/c 3T3 mouse fibroblasts and mouse lymphocytes. Evidence is presented that: i) a proportion of cholera toxin bound to GM1 of BALB/c 3T3 cells withstood extraction with Triton X-100 at 0C and remained associated with the cytoskeleton; ii) at 37C, cholera toxin-GM1 complexes were completely extracted with Triton X-100. The resulting complexes existed in a macromolecular form that did not involve other memb

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27

Poole, Deborah Marie. "Molecular analysis of plant cell wall hydrolases of bacterial origin." Thesis, University of Newcastle Upon Tyne, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238939.

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28

Omrani, M. D. "Molecular biology of a novel type of bacterial transport system." Thesis, University of Sheffield, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265584.

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29

Huen, Shing-yan Michael, and 禤承恩. "A mechanistic study of lambdaphage-mediated recombination in E. coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B35321854.

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30

Wang-Holmes, Jenny. "Molecular systematics and biodiversity of planctomycetes / Jenny Wang-Holmes (Jian-Hua Wang)." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18395.pdf.

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31

Campbell, Alan L. (Alan Lee). "The Genetics of Pigmentation in Corynebacterium poinsettiae ATCC 9682." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc330778/.

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Corynebacterium poinsettiae mutant strains blocked in carotenoid biosynthesis were obtained by treatment with the mutagen N-methyl-N1-nitro-N-nitrosoguanidine. Additional carotenoid (Crt) mutant strains were obtained from a previous study conducted in our laboratory. Fifty-nine Crt mutants affected in carotenoid biosynthesis were examined by a normal phase high performance liquid chromatography (HPLC) system. Pigment extracts of Crt mutants and C. poinsettiae wild type strains were resolved by an isocratic system with hexane:acetone:dicholoromethane, 11.35:1.73:1.00 (by vol.) as the eluting so

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32

Wong, Chi-wai Bonnie. "Essentiality of methionine aminopeptidase in staphylococcus aureus." Thesis, Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31479212.

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33

Kirke, David F. "Protein-nucleic acid interactions regulating bacterial quorum sensing." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364668.

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34

Tegmark, Wisell Karin. "Regulation of virulence gene expression in Staphylococcus aureus /." Stockholm : [Karolinska Univ. Press], 2000. http://diss.kib.ki.se/2000/91-89428-01-3/.

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35

Cheung, Lai-wan, and 張麗雲. "Evaluation of gene targets for identification of tsukamurella species." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206512.

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Using DNA-DNA hybridization as the reference method, the usefulness of six gene targets, secA, ssrA, rpoB, 16S rRNA, groEL and gyrB sequencing for identification of 28 strains isolated from human and snake, and five control strains of Tsukamurella species were evaluated. Phenotypic identification methods, using API 20C AUX and API 50 CH identification kits were also performed and analyzed. Among the 28 test strains, DNA-DNA hybridization confirmed that there were 13 strains of T. tyrosinosolvens, 15 strains of T. paurometabola. Phenotypic identification failed to differentiate the control stra

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36

Yu, Tin-Wein. "Physical and functional studies of polyketide synthase genes of Streptomyces." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260005.

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37

Lisle, Warren Derek. "Studies of the integrated functions of the phage T4 encoded Stp peptide." Thesis, University of Portsmouth, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298107.

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38

Chaithong, Thararat. "A molecular genetic investigation of enterotoxigenic factors in Bacillus cereus." Thesis, University of Strathclyde, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248351.

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39

Malik, A. N. "Genetic studies with Pseudomonas syringae pathovar pisi." Thesis, University of Greenwich, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354390.

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40

Hill, Russell. "Gene cloning studies in two nocardioform bacteria." Doctoral thesis, University of Cape Town, 1988. http://hdl.handle.net/11427/21896.

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Bibliography: pages 147-177.<br>Nocardioforms are Gram-positive, aerobic actinomycetes and are a metabolically diverse group which produce antibiotics, useful enzymes, are important in the biotransformation of organic compounds and the decomposition of organic wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of actinomycete genomic libraries. Cloning of DNA into the unique

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41

Perwez, Tariq. "The mobB protein of broad-host-range plasmid R1162 has two distinct functions, stabilization of relaxosome and enhancement of transfer /." Digital version accessible at:, 1998. http://wwwlib.umi.com/cr/utexas/main.

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42

Zhang, Xiaohong. "Cloning, sequencing, expression, and inactivation of the aminodehydroquinate dehydratase gene in Amycolatopsis mediterranei S699 /." Thesis, Connect to this title online; UW restricted, 2000. http://hdl.handle.net/1773/8602.

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43

Yu, Wai-yee Annie. "Epidemiological and emm gene analysis of non-m-typeable group A streptococcus isolates from Hong Kong." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23340204.

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44

Leung, Ka-ling. "Novel molecular targets of Burkholderia pseudomallei /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23440144.

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45

Purdy, Shona Thomson. "The biochemistry and genetics of sorbitol metabolism in clostridium pasteurianum." Thesis, Heriot-Watt University, 1991. http://hdl.handle.net/10399/870.

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46

Gibbons, Sarah Jane. "Population genetics of Wuchereria bancrofti and its bacterial endosymbiont Wolbachia." Thesis, University of Liverpool, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485943.

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Wuchereria bancrofli is a filarial parasite responsible for 90% of lymphatic filariasis worldwide. Currently a mass drug administration programme, The Global Programme for the Elimination of Lymphatic Filariasis (GPELF) is underway, through the administration of three drugs: diethylcarbamazine (DEC), albendazole and ivermectin, to eliminate lymphatic filariasis as a global he~lth problem. The major aims of the research described in this thesis, were to investigate the genetic diversity of W. bancrofli and their endosymbiotic bacteria Wolbachia and to investigate the presence of putative resist

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47

Nakajima, Erika. "Investigação de proteínas candidatas vacinais contra leptospirose. Apresentação de antígenos na forma de proteínas recombinantes purificadas ou como vacinas vivas em salmonelas atenuadas." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-02032011-173909/.

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A leptospirose é uma doença endêmica causada por Leptospiras. O genoma da Leptospira interrogans sorovar Copenhageni foi analisado para seleção de potenciais antígenos vacinais. Oito genes foram selecionados e clonados para expressão e purificação dos antígenos. A salmonela SL3261 foi usada como carregadora dos genes de leptospira em vetor pAEsox para expressão das proteínas in vivo. As salmonelas recombinantes induziram resposta imune quando administradas em camundongos por via intraperitoneal. Hamsters foram imunizados com as salmonelas, observando-se que a SLLIC10191 induziu proteção parcia

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48

Kacharia, Fenil Rashmin. "Investigating the Origin and Functions of a Novel Small RNA in Escherichia coli." PDXScholar, 2016. http://pdxscholar.library.pdx.edu/open_access_etds/3106.

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Non-coding small RNAs (sRNAs) regulate various cellular processes in bacteria. They bind to a chaperone protein Hfq for stability and regulate gene expression by base-pairing with target mRNAs. Although the importance of sRNAs in bacteria has been well established, the mode of origination of novel sRNA genes is still elusive, mainly because the rapid rate of evolution of sRNAs obscures their original sources. To overcome this impediment, we identified a recently formed sRNA (EcsR2) in E. coli, and show that it evolved from a degraded bacteriophage gene. Our analyses also revealed that young sR

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49

Stevens, James B. "The molecular genetics of iron uptake in rhizobium leguminosarum." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323075.

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50

Roberts, Daniel Paul. "Molecular mechanisms of pathogenesis incited by Erwinia carotovora subsp. carotovora." Diss., Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/71251.

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Erwinia carotovora subsp. Carotovora (Ecc) incites soft-rot on many plants. It is believed that soft-rot is due to the concerted activity of extracellular enzymes. Recombinant DNA techniques were used to study the molecular basis of pathogenesis incited by Ecc. Specifically, a clone library of Ecc strain EC14 DNA in plasmid pBR322 was constructed and transformed into Escherichia coli strain HB101. Some of the E. coli strains that contain these hybrid plasmids produce pectinases or cellulase(s). Plasmid pDR1 contains a 3.4 kilobase (kb) EC14 DNA fragment and mediates the production of endo-pect

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