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Дисертації з теми "Barley Disease and pest resistance Genetic aspects"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 28 січня 2023

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1

Collins, Nicholas C. "The genetics of barley yellow dwarf virus resistance in barley and rice." Title page, table of contents and summary only, 1996. http://hdl.handle.net/2440/46063.

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Barley yellow dwarf virus (BYDV), an aphid transmitted luteovirus, is the most widespread and economically damaging virus of cereal crops. The work in this thesis aims to characterise the basis of the naturally occurring resistance to BYDV in cereals in three ways: Firstly, by facilitating the isolation of the Yd2 gene for BYDV resistance from barley by a map-based approach. Secondly, by determining if a BYDV resistance gene in rice is orthologous to Yd2. Thirdly, by establishing if other BYDV resistance genes in non- Ethiopian barleys are allelic to Yd2. It is hoped that the information gener

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2

King, Brendon James. "Towards cloning Yd2 : a barley resistance gene to barley yellow dwarf virus." Title page, contents and summary only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phk523.pdf.

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3

Rathjen, John Paul. "Aspects of luteovirus molecular biology in relation to the interaction between BYDV-PAV and the Yd2 resistance gene of barley /." Title page, contents and summary only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phr2342.pdf.

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4

Hossain, Mohammad Abul. "Powdery mildew on barley : pathogen variability in South Australia : resistance genes in cv. Galleon /." Title page, contents and abstract only, 1986. http://web4.library.adelaide.edu.au/theses/09PH/09phh8287.pdf.

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5

Jenkin, Mandy Jane. "Genetics of boron tolerance in barley / by Mandy Jane Jenkin." Thesis, Adelaide Thesis (Ph.D.) -- University of Adelaide, Department of Plant Science, 1993. http://hdl.handle.net/2440/21652.

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6

Jenkin, Mandy Jane. "Genetics of boron tolerance in barley /." Adelaide : Thesis (Ph.D.) -- University of Adelaide, Department of Plant Science, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09phj514.pdf.

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7

Shams-Bakhsh, Masoud. "Studies on the structure and gene expression of barley yellow dwarf virus." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phs5275.pdf.

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Bibliography: leaves 118-132. This thesis examines the structure and gene expression of barley yellow dwarf viruses (BYDVs)-PAV in order to gain a better understanding of the interaction between the virus and the Yd2 resistance gene. The protein products of open reading frame (ORF)3, ORF4 and ORF5 are expressed in bacterial cells, in order to characterise the BYDV-PAV virion-associated proteins. The effect of the Yd2 resistance gene on the expression of the BYDV-PAV viral proteins in infected cells is also studied.

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8

Golegaonkar, Prashant G. "Genetic and molecular analysis of resistance to rust diseases in barley." University of Sydney, 2007. http://hdl.handle.net/2123/3549.

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Doctor of Philosophy<br>The responses of 92 barley genotypes to selected P. hordei pathotypes was assessed in greenhouse tests at seedling growth stages and in the field at adult plant growth stages to determine known or unknown resistances. On the basis of multipathotype tests, 35 genotypes were postulated to carry Rph2, Rph4, Rph5, Rph12, RphCantala alone or combinations of Rph2 + Rph4 and Rph1 + Rph2, whereas 52 genotypes lacked detectable seedling resistance to P. hordei. Five genotypes carried seedling resistance that was effective to all pathotypes tested, of which four were believed to

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9

Singh, Rampal. "Characterization of virus disease resistance in Lactuca sativa." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55529.

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Little is known about the mechanism of virus disease resistance in plants. The aim of the work presented here was to answer whether disease resistance is offered within the cell or at the level of intercellular movement of the virus. The protoplast system was used for this purpose. Conditions were optimized to isolate viable protoplasts from the leaves of Lactuca sativa cultivars. Protoplasts and leaves from resistant and susceptible Lactuca sativa cultivars were inoculated separately with turnip mosaic virus (TuMV) and lettuce mosaic virus (LMV), Virus multiplication was examined over time us

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10

Marchione, Wesley A. "Pathogen resistance genes and proteins in orchids." Virtual Press, 2003. http://liblink.bsu.edu/uhtbin/catkey/1260625.

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To study resistance (R) genes that are expressed when Sophrolaeliacattleya Ginny Champion 'Riverbend' orchid tissue was infected with the tobacco mosaic virus (TMV0), a subtraction library of cDNA clones was previously constructed using mRNA isolated before and after infection (Shuck, unpublished). From 200 clones collected, 5 clones were randomly selected, DNA was isolated, and the cDNA insert was sequenced. These sequences were imported into BLAST to search for homology to other R genes. This search revealed clone 4A to have an 84% homology to a 54 nucleotide region from the Arabidopsis thal

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11

Wang, Hongxia. "Identification of Molecular Markers Linked to X-Disease Resistance in Chokecherry." Diss., North Dakota State University, 2012. https://hdl.handle.net/10365/26565.

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X-disease, caused by phytoplasmas, is one of the destructive diseases in stone fruit trees, causing yield loss and poor fruit quality. So far no effective methods are available to control X-disease. X-disease resistance has been first discovered in chokecherry (Prunus virginiana, 2n=4x=32), which is a native woody species of North America. To identify molecular markers linked to X-disease resistance, simple sequence repeat (SSR) markers were used to construct genetic linkage maps for chokecherry and to identify markers associated with X-disease resistance in chokecherry. In this research, thre

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12

au, lars kamphuis@csiro, and Lars Gian Kamphuis. "Genetic dissection of disease resistance to Phoma medicaginis in Medicago truncatula." Murdoch University, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20090424.121918.

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Phoma medicaginis is a necrotrophic fungal pathogen, commonly found infecting Medicago truncatula and M. sativa in temperate regions of Australia. To identify, characterize and differentiate eight P. medicaginis isolates from Western Australia, morphological phenotypes and five gene regions (actin, â- tubulin, calmodulin, internal transcribed spacer, translation elongation factor 1-á) were examined. Sequence comparisons showed that specimens isolated from M. truncatula in Western Australia formed a group that was consistently different from, but closely allied to, a P. medicaginis var. medicag

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13

Ubalijoro, Eliane. "Characterization of resistance to lettuce mosaic virus in Lactuca sativa." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22821.

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Lettuce mosaic virus (LMV) is an economically important pathogen with worldwide distribution. LMV infection in L. sativa can cause significant yield losses. Resistance to LMV in L. sativa is conferred by the recessive gene mo. We attempted to position the mo gene on the L. sativa map. The ultimate goal is a better understanding of plant-virus interactions. To do so, Random Amplified Polymorphic DNA (RAPD) markers were screened in the near isogenic lines (NILs) Vanguard and Vanguard 75. These NILs differ in the presence of the mo gene in Vanguard 75. Polymorphic markers were screened for linkag

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14

Reid, Lana M. (Lana Marie). "Resistance of maize silk to Fusarium graminearum." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=70316.

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The characteristics and inheritance of maize silk resistance to Fusarium graminearum ear rot were investigated. In an in vitro test, genotypic differences in the degradation of detached silk tissue by F. graminearum were correlated to field evaluations of resistance. Susceptibility to infection decreased with silk age. Total phenolics of silk channel silk tissue increased in response to infection in resistant inbreds but decreased in susceptible inbreds. The flavones iso-orientin, iso-vitexin, maysin, luteolin, and apigenin were identified in the silk. No significant genotype by isolate intera

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15

Dufresne, Philippe J. "Development and validation of molecular markers for the detection of disease resistance alleles in Lactuca sativa." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=78352.

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In this study, RAPD (Randomly Amplified Polymorphic DNA) and SCAR (Sequence Amplified Characterized Region) markers found within 5 centiMorgans of known disease resistance loci in L. sativa were tested for their potential use in MAS. Out of thirty RAPD and SCAR markers evaluated, ten were found to be reliable predictors of disease resistance or susceptibility across a wide range of commercial and reference cultivars. Direct sequencing of seven selected markers did not reveal any significant similarity with known sequences. Three SNPs (Single Nucleotide Polymorphism) associated with two

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16

Yu, Yong Gang. "Molecular genetic analysis of host resistance to soybean mosaic virus." Diss., Virginia Tech, 1994. http://hdl.handle.net/10919/37253.

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17

Galagedara, Nelomie Nayanathara. "Identification of Quantitative Trait Loci for Resistance to Tan Spot in Durum Wheat." Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/28765.

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Tan spot, caused by Pyrenophora tritici-repentis (Ptr), is a major foliar disease on wheat. The pathosystem involves three pairs of necrotrophic effector (NE) and host sensitivity (S) gene interactions, namely Ptr ToxA-Tsn1, Ptr ToxB-Tsc2 and Ptr ToxC-Tsc1. Additionally, genetic factors conferring race-nonspecific resistance have been identified. The objectives of this study were to map tan spot resistance QTL and investigate the role of NE-S interactions in disease in durum using association and bi-parental mapping. Evaluation of a worldwide collection of durum accessions allowed identifying

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18

Horn, Marizanne. "Transfer of genetic resistance to the Russian wheat aphid from rye to wheat." Thesis, Stellenbosch : Stellenbosch University, 1997. http://hdl.handle.net/10019.1/55770.

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Thesis (MSc.) -- Stellenbosch University, 1997.<br>ENGLISH ABSTRACT: An octoploid triticale was derived from the F1 of a Russian wheat aphid resistant rye, 'Turkey 77', and 'Chinese Spring' wheat. The alloploid was crossed (a) to common wheat, and (b) to the 'Imperial' rye to 'Chinese Spring' disomic addition lines. F2 progeny from these crosses were tested for Russian wheat aphid resistance and C-banded. Resistance was found to be associated with chromosome arm 1RS of the 'Turkey 77' rye genome. This initial work was done by MARAIS (1991) who made a RWA resistant, monotelosomic 1RS (

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19

Brière, Stéphan C. "Selection of partial resistance for crown rust (Puccinia ćoronata Cda.) race 264 in oat." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60678.

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Nineteen cultivars and fourteen breeding lines were evaluated for partial resistance to crown rust Puccinia coronata race 264. Multivariate statistical methods such as principal component and cluster analyses were employed to identify significant resistance parameters and to group oat genotypes with similar rust resistance characteristics. This involved two separate investigations consisting of two experiments each, the first experiment conducted under field conditions and the second conducted under growth bench conditions. From both of the investigations a group of oat genotypes with high par

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20

Abu-Nada, Yousef. "Metabolic profiling of potato cultivars varying in horizontal resistance to late blight, Phytophthora infestans." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102947.

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Potato is one of the most important crops grown in Canada and all over the world. Late blight caused by P. infestans is one of the major diseases of potato and is mainly managed by fungicides application. The extensive use of fungicides not only causes adverse effects on the environment but also accelerates the development of resistance in this pathogen. Horizontal resistance is considered as the best choice to control P. infestans as it is durable over years. Breeding for durable resistance requires evaluation of hundreds of breeding lines in greenhouses and in the field. This is usually done

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21

Van, Staden Derick. "AFLP and PCR markers for the Ht1, Ht2, Ht3 and Htn1 resistance genes in maize." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52078.

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Thesis (PhDAgric)--University of Stellenbosch, 2001.<br>ENGLISH ABSTRACT: Maize is undoubtedly South Africa's most important field crop. The identification of markers and genes for traits of interest is important to sustain the improvement of maize cultivation. Northern corn leaf blight (NClB) is a disease that occurs worldwide and can dramatically reduce yield. A number of single dominant resistance genes have been identified for NClB and some have been mapped. Currently there are no simple PCR markers for any of these resistance genes, making markerassisted selection (MAS) difficult.

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22

Lehmensiek, Anke. "Genetic mapping of gray leaf spot resistance genes in maize." Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51776.

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Thesis (PhD)--Stellenbosch University, 2000.<br>ENGLISH ABSTRACT: Gray leaf spot (GLS) of maize, caused by the fungus Cercospora zeae-maydis, can reduce grain yields by up to 60% and it is now recognized as one of the most significant yield-limiting diseases of maize in many parts of the world. The most sustainable and long-term management strategy for GLS will rely heavily on the development of high-yielding, locally adapted GLS resistant hybrids. Molecular markers could be useful to plant breeders to indirectly select for genes affecting GLS resistance and to identify resistance genes

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23

Krsikapa, Nenad. "Variation for resistance to Fusarium graminearum ear rot in selfed families from the corn population Zapalote Chico." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0021/MQ37137.pdf.

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24

Marino, Dante. "Screening of Germplasm Accessions from the Brassica Species for Resistance against PG3 and PG4 Isolates of Blackleg." Thesis, North Dakota State University, 2011. https://hdl.handle.net/10365/29053.

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Blackleg is a disease of canola and rapeseed cultivars that is caused by the fungus Leptosphaeria maculans (Desm.) Ces. & de Not., and it is by far the most destructive pathogen of canola in North America. In recent years, blackleg strains belonging to pathogenicity groups (PG) 3 and 4 have been discovered in North Dakota. Recent outbreaks of the disease have added a sense of urgency to characterize the risk these new strains represent for the canola industry and to identify sources of resistance against them. Thus, the objectives of this study were to screen germplasm collections of Brassica

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25

Hinga, Clark D. "Genetic analysis of resistance to Myzus persicae (Sulzer) in Nicotiana tabacum L." Thesis, Virginia Tech, 1985. http://hdl.handle.net/10919/41552.

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<p>Field experiments with the green peach aphid on tobacco were conducted at Blackstone, Virginia in 1983 and 1984. The objectives were to: 1) confirm and identify source materials resistant to the green peach aphid; 2) study the inheritance of aphid resistance; 3) verify heritability of resistant genotypes through F2 and advanced generation testing and 4) investigate the nature of the resistance.</p> <p> Results showed green peach aphid resistance in Tobacco Introductions 1462, 1118, 1112, 1024, breeding line 1-35 and cultivar NC 745. Inheritance studies showed that the resistance is a recess

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26

Bierman, Anandi. "Mapping and survey sequencing of Dn resistance genes in Triticum aestivum L." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96912.

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Thesis (PhD)--Stellenbosch University, 2015<br>ENGLISH ABSTRACT : Diuraphis noxia Kurdjumov (Russian Wheat Aphid; RWA) is a pest of wheat and barley that has spread from its home range in the fertile crescent to most wheat producing countries except Australia. Since its first introduction to South Africa and the USA in the late 20th century, breeding programs for wheat phenotypes resistant to the aphid were put in place. Conventional breeding practices rely on phenotypic screening to verify traits carried by offspring and genetic tools such as marker assisted selection (MAS) have greatly aide

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27

Montesclaros, Luz B. "Mapping of molecular markers surrounding the Tu gene conferring resistance to turnip mosaic virus in Lactuca sativa L." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23923.

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In lettuce (Lactuca sativa), the dominant gene Tu confers resistance to turnip mosaic virus (TuMV) infection. In order to eventually clone and characterize the Tu gene using a map-based cloning strategy, the chromosome region in which Tu is located needs to be saturated with molecular markers. Random polymorphic DNA (RAPD) markers were screened using bulked segregant analysis. Nine new RAPD markers, UBC431$ rm sb{420}, UBC431 sb{940}, UBC434 sb{360}, UBC434 sb{1000}, UBC439 sb{520}, UBC448 sb{685:750}, UBC135 sb{240}, OP108 sb{410} and OP108 sb{1305},$ were identified as linked to Tu. Each mar

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28

Williams, Kevin John. "Biological and genetic studies of wheat resistance to Heterodera avenae." Title page, summary and contents only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phw7238.pdf.

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29

Deng, Yinghai 1966. "Development and disease resistance of leafy reduced stature maize (Zea mays L.)." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38177.

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Previous studies on Leafy reduced-stature (LRS) maize found that it had extremely early maturity and a higher harvest index (HI), leading to high yields for its maturity rating. Whether this apparent high HI is relaxed to its earliness, or can also exist among the medium or late maturity LRS maize has not been previously investigated. It was also of interest to know if the traits that produced the LRS canopy structure have pleiotropic effects on root architecture. Finally, field observations indicated that LRS maize had a lower incidence of common smut. It is not known whether this apparent re

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30

Robbins, Marjorie. "The location of Tu on the genetic map of Lactuca sativa and the identification of random amplified polymorphic DNA markers flanking and tightly linked to Tu /." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69684.

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In Lactuca sativa, the dominant gene Tu confers resistance to infection by turnip mosaic virus (TuMV). Tu and Dm5/8, a gene for resistance to Bremia lactucae, are linked in L. sativa. The area surrounding Dm5/8 on the genetic map of L. sativa contains restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. The orientation of Tu relative to Dm5/8 was not known. Locating Tu would indicate which markers are on the map of lettuce close to Tu. To locate Tu on the L. sativa genetic map, F$ sb3$ families from recombinant F$ sb2$ in the Dm5/8 area of a cros

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31

Van, Eeden C. (Christiaan). "The construction of gene silencing transformation vectors for the introduction of multiple-virus resistance in grapevines." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53764.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Viruses are some of the most important pathogens of grapevines. There are no effective chemical treatments, and no grapevine- or other natural resistance genes have been discovered against grapevine infecting viruses. The primary method of grapevine virus control is prevention by biological indexing and molecular- and serological screening of rootstocks and scions before propagation. Due to the spread of grapevine viruses through insect vectors, and in the case of GRSPaV the absence of serological screening, these metho

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32

Joubert, Dirk Albert 1973. "Regulation of the Vitis vinifera PGIP1 gene encoding a polygalacturonase-inhibiting protein." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53759.

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Thesis (PhD)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Plant-pathogen interactions have been intensively investigated in the last decade. This major drive towards understanding the fundamental aspects involved in plant disease resistance is propelled by the obvious agricultural and economical benefits that are intrinsically linked to disease and stress resistant plants. It is, therefore, not surprising that fundamental research in this area is not just restricted to model organisms, such as Arabidopsis and tobacco, but also extends to more traditional crop plants, such as

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33

Spaner, Dean Michael. "Agronomic evaluation of short season quality protein maize." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61042.

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The introduction of Quality Protein Maize (QPM), hard endosperm opaque-2 maize, into northern temperate maize growing areas is a desirable breeding objective. In topcrosses with opaque-2 testers, in diallel combination, as inbreds per se, and in inbred disease screening nurseries, some QPM lines performed better than or equal to the best local checks. In general, while agronomic potential is high for some lines and gains from selection are statistically possible, longer days to flowering intervals and higher levels of moisture at harvest than check hybrids indicated a need to improve adaptatio

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34

Schuck, Heather A. "Differentially expressed genes of Sophrolaeliacattleya Ginny Champion "Riverbend" in response to the odontoglossum ringspot virus." Virtual Press, 2000. http://liblink.bsu.edu/uhtbin/catkey/1164841.

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Due to the rapid destruction of native orchid habitats it has become necessary to house many endangered orchid species in greenhouse environments where enhanced spread of viral disease occurs due to the close contact between plants. This research was concerned with the construction of a library of genes whose expression is induced in response to viral challenge. In uncovering the genes that are activated during plant-pathogen interactions, it may be possible to manipulate these pathways to develop virus resistant orchids. Furthermore, this research will contribute additional information for th

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35

Heyns, I. C. "Mapping and restructuring of an Ae. kotschyi derived translocation segment in common wheat." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5172.

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Thesis (PhD (Genetics))--University of Stellenbosch, 2010.<br>Includes bibliography.<br>ENGLISH ABSTRACT: The wild relatives are an important source of new genes for the genetic improvement of wheat. At Stellenbosch University the leaf and stripe rust resistance genes Lr54 and Yr37 were transferred from Aegilops kotschyi to chromosome 2DL of wheat. In an attempt to reduce the size of the whole-arm translocation on which the resistance genes occur, homoeologous pairing was induced between the wheat and corresponding Ae. kotschyi chromatin. The purpose of this study was to: (i) Evaluate th

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36

Filkowski, Jody, and University of Lethbridge Faculty of Arts and Science. "The effect of pathogens on plant genome stability." Thesis, Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, 2004, 2004. http://hdl.handle.net/10133/254.

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Resistance (R) genes, a key factor in determining the resistance of plants, have been shown often to be highly allelic entities existing in duplicated regions of the genome. This characteristic suggests that R-gene acquisition may have arisen through frequent genetic rearrangements as a result of transient, reduced genome stability. Tabacco plants transgenic for a recombination construct exhibited reduced genome stability upon infection with a virulent pathogen (tobacco mosaic virus). The reduced genome stability manifested as an increase in recombination events in the transgene. Such increase

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37

Beeck, Cameron. "Simultaneous improvement in black spot resistance and stem strength in field pea (Pisum sativum L.)." University of Western Australia. School of Plant Biology, 2006. http://theses.library.uwa.edu.au/adt-WU2006.0057.

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[Truncated abstract] Field pea (Pisum sativum) has many benefits when included in the crop rotation system in broadacre grain farming. These benefits include a disease break and improved weed control for cereals and less dependence on nitrogenous fertilisers due to the leguminous nature of pea. Currently, field pea adoption in Australia is low because the crop is susceptible to the fungal disease `black spot’ (Mycosphaerella pinodes) and has low stem strength and a lodged canopy. Black spot causes yield losses averaging 10-15% per year. Lodging results in difficult and costly harvesting, incre

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Saxon, Herbert. "The molecular biology of orchids : transformation by Agrobacterium Tumefaciens and DNA fingerprinting." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/941575.

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The work reported here was done at the Wheeler Orchid Collection and Species Bank and the Department of Biology at Ball State University. We have developed a research teaching program with two applied research goals: genetically transforming and DNA fingerprinting orchid tissue. As part of their molecular biology education, students have investigated the genetic transformation of orchids for mitigating viral symptoms and the identification of unknown orchids by DNA fingerprinting. In a second application of the technology, DNA fingerprinting has been used to determine evolutionary relationship

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39

Khan, Imtiaz Ahmed. "Utilisation of molecular markers in the selection and characterisation of wheat-alien recombiant chromosomes." Title page, contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phk451.pdf.

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Bibliography: leaves 137-163. his is a comprehensive study of induced homoeologous recombination along most of the complete genetic length of two homoeologous chromosomes in the Triticeae (7A of common wheat and 7Ai of Agropyron intermedium), using co-dominant DNA markers. Chromosome 7Ai was chosen as a model alien chromosome because is has been reported to carry agronomically important genes conferring resistance to stem rust and barley yellow dwarf virus on its short and long arms, respectively.

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40

Ramburan, Viresh Premraj. "Genetic mapping of adult plant stripe rust resistance in the wheat cultivar Kariega." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53438.

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Thesis (PhD (Agric)) -- Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: Stripe (yellow) rust of wheat, caused by Puccinia striiformis f.sp. tritici, was first detected as a single introduction into South Africa in 1996. Two additional pathotypes have since been identified. Control of the disease may be achieved by use of genetic adult plant resistance (APR) as is present in the local cultivar 'Kariega'. The aim of this project was to understand the genetic basis of the APR in 'Kariega' to facilitate breeding of new varieties with genetic resistance to stripe rust. A partial linkage

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41

Carroll, Audra L. "Sense and antisense oligonucleotide inhibition of the Odontoglossum ringspot virus (ORSV) coat protein gene via microprojectile bombardment of orchid callus tissue." Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1210536.

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A major goal of our laboratory is to confer resistence specifically to the Odontoglossum ringspot virus [ORSV; sometimes referred to as tobacco mosaic virus strain O (TMV-O)] in orchids. The chosen strategy may also provide cross-protection to other pathogens. The experimental design for the entire project is presented here along with the results obtained in several preliminary experiments performed in this research. Our approach involved RT-PCR amplification of the viral coat protein gene with gene-specific primers and digestion of the cDNAs into oligonucleotides. These fragments were cl

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42

Collins, Nicholas Charles. "The genetics of barley yellow dwarf virus resistance in barley and rice." Thesis, 1996. http://hdl.handle.net/2440/46063.

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Barley yellow dwarf virus (BYDV), an aphid transmitted luteovirus, is the most widespread and economically damaging virus of cereal crops. The work in this thesis aims to characterise the basis of the naturally occurring resistance to BYDV in cereals in three ways: Firstly, by facilitating the isolation of the Yd2 gene for BYDV resistance from barley by a map-based approach. Secondly, by determining if a BYDV resistance gene in rice is orthologous to Yd2. Thirdly, by establishing if other BYDV resistance genes in non- Ethiopian barleys are allelic to Yd2. It is hoped that the information gener

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43

March, Timothy. "The genetic basis of barley black point formation." 2008. http://hdl.handle.net/2440/50740.

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Black point of barley grain refers to a discolouration of the embryo end of the grain. Historically black point has been proposed to be due to fungal colonisation of the grain. However, Koch’s postulates have yet to be satisfied. The discolouration occurs during grain fill in response to high humidity or rainfall during the grain filling period. In wheat, which is also affected by black point, the discolouration has been proposed to be due to the oxidation of phenolic acids within the grain to form discoloured end products. Within this study, two approaches were investigated in order to unders

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44

March, Timothy. "The genetic basis of barley black point formation." Thesis, 2008. http://hdl.handle.net/2440/50740.

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Black point of barley grain refers to a discolouration of the embryo end of the grain. Historically black point has been proposed to be due to fungal colonisation of the grain. However, Koch’s postulates have yet to be satisfied. The discolouration occurs during grain fill in response to high humidity or rainfall during the grain filling period. In wheat, which is also affected by black point, the discolouration has been proposed to be due to the oxidation of phenolic acids within the grain to form discoloured end products. Within this study, two approaches were investigated in order to unde

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45

Abbott, David Colin. "Pyramiding scald resistance genes in barley." Phd thesis, 1997. http://hdl.handle.net/1885/145297.

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46

Castro, Tabo Ariel Julio. "Stripe rust resistance pyramids in barley." Thesis, 2002. http://hdl.handle.net/1957/29789.

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47

Toojinda, Theeryut. "Mapping and introgression of disease resistance genes in barley (Hordeum vulgare L.)." Thesis, 1998. http://hdl.handle.net/1957/33275.

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Molecular tools, coupled with unique germplasm stocks and rigorous phenotyping, are useful for developing a better understanding of qualitative and quantitative disease resistance genes in plants. The identification of molecular markers linked to all types of resistance genes provides opportunities for implementing a range of resistance breeding strategies, ranging from gene pyramiding to gene deployment. This thesis consists of two chapters. The first describes a disease resistance gene mapping effort and the second describes a disease resistance gene introgression effort. The number, locatio

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Prehn, Doris A. "Analysis of genetic resistance to barley stripe rust (Puccinia striiformis f. sp. hordei)." Thesis, 1993. http://hdl.handle.net/1957/36114.

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Stripe rust (Puccinia striiformis f. sp. hordei) is a serious disease of barley that can cause up to 70% yield loss in susceptible varieties. The fungus is moving northward, threatening major barley production areas in the US, where most cultivars are susceptible. Fungicides are available for control of stripe rust, but economic and environmental considerations favor genetic resistance. Two stripe rust resistance quantitative trait loci (QTLs) located in chromosomes 4 and 7 have previously been reported. One hundred and ten doubled haploid progeny from a stripe rust susceptible x resistant cro

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Paltridge, Nicholas G. (Nicholas Geoffrey). "The development of molecular markers for barley Yd2, the barley yellow dwarf virus resistance gene." 1998. http://web4.library.adelaide.edu.au/theses/09APSP/09apspp183.pdf.

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Includes bibliographical references (l5 leaves) The aim of the work presented in this thesis was to develop molecular genetic markers for YD2 (the gene in barley which provides protection against barley yellow dwarf luteovirus) which could be used for the marker assisted selection of the gene in breeding programs and enable the gene to be cloned via a map-based approach.

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50

Choe, Y. W. (Young Won). "DNA markers for cereal cyst nematode (Heterodera avenae Woll.) resistance gene in barley." 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phc545.pdf.

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