Статті в журналах: "Branched1 (brc1)"

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Shen, Junjun, Yaqi Zhang, Danfeng Ge, Zhongyi Wang, Weiyuan Song, Ran Gu, Gen Che, Zhihua Cheng, Renyi Liu, and Xiaolan Zhang. "CsBRC1 inhibits axillary bud outgrowth by directly repressing the auxin efflux carrier CsPIN3 in cucumber." Proceedings of the National Academy of Sciences 116, no. 34 (August 7, 2019): 17105–14. http://dx.doi.org/10.1073/pnas.1907968116.

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Shoot branching is an important agronomic trait that directly determines plant architecture and affects crop productivity. To promote crop yield and quality, axillary branches need to be manually removed during cucumber production for fresh market and thus are undesirable. Auxin is well known as the primary signal imposing for apical dominance and acts as a repressor for lateral bud outgrowth indirectly. The TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) family gene BRANCHED1 (BRC1) has been shown to be the central integrator for multiple environmental and developmental factors that functions locally to inhibit shoot branching. However, the direct molecular link between auxin and BRC1 remains elusive. Here we find that cucumber BRANCHED1 (CsBRC1) is expressed in axillary buds and displays a higher expression level in cultivated cucumber than in its wild ancestor. Knockdown of CsBRC1 by RNAi leads to increased bud outgrowth and reduced auxin accumulation in buds. We further show that CsBRC1 directly binds to the auxin efflux carrier PIN-FORMED (CsPIN3) and negatively regulates its expression in vitro and in vivo. Elevated expression of CsPIN3 driven by the CsBRC1 promoter results in highly branched cucumber with decreased auxin levels in lateral buds. Therefore, our data suggest that CsBRC1 inhibits lateral bud outgrowth by direct suppression of CsPIN3 functioning and thus auxin accumulation in axillary buds in cucumber, providing a strategy to breed for cultivars with varying degrees of shoot branching grown in different cucumber production systems.

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Shim, Sangrea, Jungmin Ha, Moon Young Kim, Man Soo Choi, Sung-Taeg Kang, Soon-Chun Jeong, Jung-Kyung Moon, and Suk-Ha Lee. "GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)." International Journal of Molecular Sciences 20, no. 1 (January 1, 2019): 135. http://dx.doi.org/10.3390/ijms20010135.

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Branch number is one of the main factors affecting the yield of soybean (Glycine max (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs) were associated with branch numbers in a soybean core collection. Among these QTNs, a linkage disequilibrium (LD) block qtnBR6-1 spanning 20 genes was found to overlap a previously identified major quantitative trait locus qBR6-1. To validate and narrow down qtnBR6-1, we developed a set of near-isogenic lines (NILs) harboring high-branching (HB) and low-branching (LB) alleles of qBR6-1, with 99.96% isogenicity and different branch numbers. A cluster of single nucleotide polymorphisms (SNPs) segregating between NIL-HB and NIL-LB was located within the qtnBR6-1 LD block. Among the five genes showing differential expression between NIL-HB and NIL-LB, BRANCHED1 (BRC1; Glyma.06G210600) was down-regulated in the shoot apex of NIL-HB, and one missense mutation and two SNPs upstream of BRC1 were associated with branch numbers in 59 additional soybean accessions. BRC1 encodes TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 and 2 transcription factor and functions as a regulatory repressor of branching. On the basis of these results, we propose BRC1 as a candidate gene for branching in soybean.

3

González-Grandío, Eduardo, Alice Pajoro, José M. Franco-Zorrilla, Carlos Tarancón, Richard G. H. Immink, and Pilar Cubas. "Abscisic acid signaling is controlled by a BRANCHED1/HD-ZIP I cascade in Arabidopsis axillary buds." Proceedings of the National Academy of Sciences 114, no. 2 (December 27, 2016): E245—E254. http://dx.doi.org/10.1073/pnas.1613199114.

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Shoot-branching patterns determine key aspects of plant life and are important targets for crop breeding. However, we are still largely ignorant of the genetic networks controlling locally the most important decision during branch development: whether the axillary bud, or branch primordium, grows out to give a lateral shoot or remains dormant. Here we show that, inside the buds, the TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) transcription factor BRANCHED1 (BRC1) binds to and positively regulates the transcription of three related Homeodomain leucine zipper protein (HD-ZIP)-encoding genes: HOMEOBOX PROTEIN 21 (HB21), HOMEOBOX PROTEIN 40 (HB40), and HOMEOBOX PROTEIN 53 (HB53). These three genes, together with BRC1, enhance 9-CIS-EPOXICAROTENOID DIOXIGENASE 3 (NCED3) expression, lead to abscisic acid accumulation, and trigger hormone response, thus causing suppression of bud development. This TCP/HD-ZIP genetic module seems to be conserved in dicot and monocotyledonous species to prevent branching under light-limiting conditions.

4

Xia, Xiaojian, Han Dong, Yanling Yin, Xuewei Song, Xiaohua Gu, Kangqi Sang, Jie Zhou, et al. "Brassinosteroid signaling integrates multiple pathways to release apical dominance in tomato." Proceedings of the National Academy of Sciences 118, no. 11 (March 8, 2021): e2004384118. http://dx.doi.org/10.1073/pnas.2004384118.

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The control of apical dominance involves auxin, strigolactones (SLs), cytokinins (CKs), and sugars, but the mechanistic controls of this regulatory network are not fully understood. Here, we show that brassinosteroid (BR) promotes bud outgrowth in tomato through the direct transcriptional regulation of BRANCHED1 (BRC1) by the BR signaling component BRASSINAZOLE-RESISTANT1 (BZR1). Attenuated responses to the removal of the apical bud, the inhibition of auxin, SLs or gibberellin synthesis, or treatment with CK and sucrose, were observed in bud outgrowth and the levels of BRC1 transcripts in the BR-deficient or bzr1 mutants. Furthermore, the accumulation of BR and the dephosphorylated form of BZR1 were increased by apical bud removal, inhibition of auxin, and SLs synthesis or treatment with CK and sucrose. These responses were decreased in the DELLA-deficient mutant. In addition, CK accumulation was inhibited by auxin and SLs, and decreased in the DELLA-deficient mutant, but it was increased in response to sucrose treatment. CK promoted BR synthesis in axillary buds through the action of the type-B response regulator, RR10. Our results demonstrate that BR signaling integrates multiple pathways that control shoot branching. Local BR signaling in axillary buds is therefore a potential target for shaping plant architecture.

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Wang, Ming, Laurent Ogé, Linda Voisine, Maria-Dolores Perez-Garcia, Julien Jeauffre, Laurence Hibrand Saint-Oyant, Philippe Grappin, Latifa Hamama, and Soulaiman Sakr. "Posttranscriptional Regulation of RhBRC1 (Rosa hybrida BRANCHED1) in Response to Sugars is Mediated via its Own 3′ Untranslated Region, with a Potential Role of RhPUF4 (Pumilio RNA-Binding Protein Family)." International Journal of Molecular Sciences 20, no. 15 (August 4, 2019): 3808. http://dx.doi.org/10.3390/ijms20153808.

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The shoot branching pattern is a determining phenotypic trait throughout plant development. During shoot branching, BRANCHED1 (BRC1) plays a master regulator role in bud outgrowth, and its transcript levels are regulated by various exogenous and endogenous factors. RhBRC1 (the homologous gene of BRC1 in Rosa hybrida) is a main branching regulator whose posttranscriptional regulation in response to sugar was investigated through its 3′UTR. Transformed Rosa calluses containing a construction composed of the CaMV35S promoter, the green fluorescent protein (GFP) reporter gene, and the 3′UTR of RhBRC1 (P35S:GFP::3′UTRRhBRC1) were obtained and treated with various combinations of sugars and with sugar metabolism effectors. The results showed a major role of the 3′UTR of RhBRC1 in response to sugars, involving glycolysis/the tricarboxylic acid cycle (TCA) and the oxidative pentose phosphate pathway (OPPP). In Rosa vegetative buds, sequence analysis of the RhBRC1 3′UTR identified six binding motifs specific to the Pumilio/FBF RNA-binding protein family (PUF) and probably involved in posttranscriptional regulation. RhPUF4 was highly expressed in the buds of decapitated plants and in response to sugar availability in in-vitro-cultured buds. RhPUF4 was found to be close to AtPUM2, which encodes an Arabidopsis PUF protein. In addition, sugar-dependent upregulation of RhPUF4 was also found in Rosa calluses. RhPUF4 expression was especially dependent on the OPPP, supporting its role in OPPP-dependent posttranscriptional regulation of RhBRC1. These findings indicate that the 3′UTR sequence could be an important target in the molecular regulatory network of RhBRC1 and pave the way for investigating new aspects of RhBRC1 regulation.

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Lv, Xiang, Mingsheng Zhang, Xiaolan Li, Ruihua Ye, and Xiaohong Wang. "Transcriptome Profiles Reveal the Crucial Roles of Auxin and Cytokinin in the “Shoot Branching” of Cremastra appendiculata." International Journal of Molecular Sciences 19, no. 11 (October 26, 2018): 3354. http://dx.doi.org/10.3390/ijms19113354.

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Cremastra appendiculata has become endangered due to reproductive difficulties. Specifically, vegetative reproduction is almost its only way to reproduce, and, under natural conditions, it cannot grow branches, resulting in an extremely low reproductive coefficient (reproductive percentage). Here, we performed RNA-Seq and a differentially expressed gene (DEG) analysis of the three stages of lateral bud development in C. appendiculata after decapitation—dormancy (D2), transition (TD2), and emergence (TG2)—and the annual axillary bud natural break (G1) to gain insight into the molecular regulatory network of shoot branching in this plant. Additionally, we applied the auxin transport inhibitors N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodibenzoic acid (TIBA) to a treated pseudobulb string of C. appendiculata to verify the conclusions obtained by the transcriptome. RNA-Seq provided a wealth of valuable information. Successive pairwise comparative transcriptome analyses revealed 5988 genes as DEGs. GO (Gene Ontology) and KEGG (Kyoto encyclopedia of genes and genomes) analyses of DEGs showed significant enrichments in phytohormone biosynthesis and metabolism, regulation of hormone levels, and a hormone-mediated signaling pathway. qRT-PCR validation showed a highly significant correlation (p < 0.01) with the RNA-Seq generated data. High-performance liquid chromatography (HPLC) and qRT-PCR results showed that, after decapitation, the NPA- and TIBA-induced lateral buds germinated due to rapidly decreasing auxin levels, caused by upregulation of the dioxygenase for auxin oxidation gene (DAO). Decreased auxin levels promoted the expression of isopentenyl transferase (IPT) and cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A) genes and inhibited two carotenoid cleavage dioxygenases (CCD7 and CCD8). Zeatin levels significantly increased after the treatments. The increased cytokinin levels promoted the expression of WUSCHEL (WUS) and inhibited expression of BRANCHED1 (BRC1) in the cytokinin signal transduction pathway and initiated lateral bud outgrowth. Our data suggest that our theories concerning the regulation of shoot branching and apical dominance is really similar to those observed in annual plants. Auxin inhibits bud outgrowth and tends to inhibit cytokinin levels. The pseudobulb in the plant behaves in a similar manner to that of a shoot above the ground.

7

Jiang, Hongxin, Guimei Han, Yaping Xu, Junxing Li, Xiaowei Liu, and Deming Kong. "A fluorescent biosensor for highly specific and ultrasensitive detection of adenosine triphosphate based on ligation-triggered branched rolling circle amplification." Analytical Methods 11, no. 36 (2019): 4629–36. http://dx.doi.org/10.1039/c9ay01482a.

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A fluorescent sensing strategy for ultrasensitive and highly selective detection of ATP was presented by taking advantage of the exponential amplification capability of BRCA and the extreme specificity of T4 DNA ligase toward ATP.

8

Arason, Agnarsson, Johannesdottir, Johannsson, Hilmarsdottir, Reynisdottir, and Barkardottir. "The BRCA1 c.4096+3A>G Variant Displays Classical Characteristics of Pathogenic BRCA1 Mutations in Hereditary Breast and Ovarian Cancers, But Still Allows Homozygous Viability." Genes 10, no. 11 (November 1, 2019): 882. http://dx.doi.org/10.3390/genes10110882.

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Mutations in BRCA1 result in predisposal to breast and ovarian cancers, but many variants exist with unknown clinical significance (VUS). One is BRCA1 c.4096+3A>G, which affects production of the full-length BRCA1 transcript, while augmenting transcripts lacking most or all of exon 11. Nonetheless, homozygosity of this variant has been reported in a healthy woman. We saw this variant cosegregate with breast and ovarian cancer in several family branches of four Icelandic pedigrees, with instances of phenocopies and a homozygous woman with lung cancer. We found eight heterozygous carriers (0.44%) in 1820 unselected breast cancer cases, and three (0.15%) in 1968 controls (p = 0.13). Seeking conclusive evidence, we studied tumors from carriers in the pedigrees for wild-type-loss of heterozygosity (wtLOH) and BRCA1-characteristic prevalence of estrogen receptor (ER) negativity. Of 15 breast and six ovarian tumors, wtLOH occurred in nine breast and all six ovarian tumours, and six of the nine breast tumors with wtLOH were ER-negative. These data accord with a pathogenic BRCA1-mutation. Our findings add to the current knowledge of BRCA1, and the role of its exon 11 in cancer pathogenicity, and will be of use in clinical genetic counselling.

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Basha, S. Jaffar, V. Jayalakshmi, S. Khayum Ahammed, and N. Kamakshi. "Studies on growth and yield characters of chickpea (Cicer arietinum L.) varieties suitable for mechanical harvesting." Tropical Plant Research 7, no. 3 (December 31, 2020): 634–37. http://dx.doi.org/10.22271/tpr.2020.v7.i3.079.

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The growth and yield characters of chickpea varieties suitable for mechanical harvesting were evaluated through field experiment conducted for three consecutive years (2016__17 to 2018__19) during rabi season on vertisols under rainfed conditions at Regional Agricultural Research Station, Nandyal, Andhra Pradesh. The investigation was carried out in split plot design with three replications. Two plant geometries (30.0 × 10.0 cm and 22.5 × 10.0 cm) were assigned to main plots and six chickpea varieties (viz., GBM 2, Dheera, CSJ 515, HC 5, Phule G 08108 and BRC 1) were assigned to sub plots. Pooled analysis of experimental results indicated that significantly higher number of branches per plant (8.7) and number of pods per plant (31.1) and test weight (24.3 g) were observed under 30.0 × 10.0 cm when compared to 22.5 × 10.0 cm. Higher plant height (44.8 cm), height of lowest pod bearing branch (30.0 cm), lower days to 50 % flowering (42.1 days) and higher test weight (31.2 g) were observed in Dheera. Higher number of branches per plant (9.2) and number of pods per plant (34.2) were observed in GBM 2. Higher seed yield was observed in Phule G 08108 (1708 kg ha-1) which is followed by GBM 2 (1675 kg ha-1) Dheera (1569 kg ha-1) and BRC 1 (1493 kg ha-1). Higher harvest index (56.4%) was also observed in Phule G 08108. Chickpea varieties GBM2, Dheera and BRC1 were best suitable for mechanical harvesting and higher seed yield due to their excellent morphology.

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Wei, Wei, Junichi Inaba, Yan Zhao, Joseph D. Mowery, and Rosemarie Hammond. "Phytoplasma Infection Blocks Starch Breakdown and Triggers Chloroplast Degradation, Leading to Premature Leaf Senescence, Sucrose Reallocation, and Spatiotemporal Redistribution of Phytohormones." International Journal of Molecular Sciences 23, no. 3 (February 5, 2022): 1810. http://dx.doi.org/10.3390/ijms23031810.

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Witches’-broom (WB, excessive initiation, and outgrowth of axillary buds) is one of the remarkable symptoms in plants caused by phytoplasmas, minute wall-less intracellular bacteria. In healthy plants, axillary bud initiation and outgrowth are regulated by an intricate interplay of nutrients (such as sugars), hormones, and environmental factors. However, how these factors are involved in the induction of WB by phytoplasma is poorly understood. We postulated that the WB symptom is a manifestation of the pathologically induced redistribution of sugar and phytohormones. Employing potato purple top phytoplasma and its alternative host tomato (Solanum lycopersicum), sugar metabolism and transportation, and the spatiotemporal distribution of phytohormones were investigated. A transmission electron microscopy (TEM) analysis revealed that starch breakdown was inhibited, resulting in the degradation of damaged chloroplasts, and in turn, premature leaf senescence. In the infected source leaves, two marker genes encoding asparagine synthetase (Sl-ASN) and trehalose-6-phosphate synthase (Sl-TPS) that induce early leaf senescence were significantly up-regulated. However, the key gibberellin biosynthesis gene that encodes ent-kaurene synthase (Sl-KS) was suppressed. The assessment of sugar content in various infected tissues (mature leaves, stems, roots, and leaf axils) indicated that sucrose transportation through phloem was impeded, leading to sucrose reallocation into the leaf axils. Excessive callose deposition and the resulting reduction in sieve pore size revealed by aniline blue staining and TEM provided additional evidence to support impaired sugar transport. In addition, a spatiotemporal distribution study of cytokinin and auxin using reporter lines detected a cytokinin signal in leaf axils where the axillary buds initiated. However, the auxin responsive signal was rarely present in such leaf axils, but at the tips of the newly elongated buds. These results suggested that redistributed sucrose as well as cytokinin in leaf axils triggered the axillary bud initiation, and auxin played a role in the bud elongation. The expression profiles of genes encoding squamosa promoter-binding proteins (Sl-SBP1), and BRANCHED1 (Sl-BRC1a and Sl-BRC1b) that control axillary bud release, as determined by quantitative reverse transcription (qRT)-PCR, indicated their roles in WB induction. However, their interactions with sugars and cytokinins require further study. Our findings provide a comprehensive insight into the mechanisms by which phytoplasmas induce WB along with leaf chlorosis, little leaf, and stunted growth.

11

Cao, Yaoliang, Yanlong Dong, Runming Zhang, Qian Li, Ruonan Peng, Chao Chen, Mengdi Lu, and Xiaoxia Jin. "Cucumber Strigolactone Receptor CsDAD2 and GA3 Interact to Regulate Shoot Branching in Arabidopsis thaliana L." Horticulturae 9, no. 1 (December 23, 2022): 23. http://dx.doi.org/10.3390/horticulturae9010023.

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Previous studies identified that strigolactones (SLs) and gibberellins (GAs) interacted when controlling branching in plant shoots, but the underlying mechanism remains unknown. qRT-PCR analysis suggested that the SL receptor gene CsDAD2 was significantly upregulated in the leaves, stems, and nodes of cucumber after treatment with 50 mg/L of GA3. Furthermore, the CsDAD2 gene was cloned and introduced into wild-type Arabidopsis plants via Agrobacterium-mediated transformation. For the CsDAD2-OE lines, the endogenous content of GA3 was subsequently higher at the seedling stage, with the number of primary cauline branches also significantly increased at the maturity stage compared with WT. Additionally, GA-related genes were up-regulated in the first inter-nodes and the third nodes of the CsDAD2-OE lines, thus indicating that GA was metabolically active in these tissues. The expression of the branch inhibitor gene AtBRC1 decreased at the seedling stage as well as at the maturity stage of the CsDAD2-OE lines. These findings suggest that CsDAD2 might have important functions in the interactions between GAs and SLs as it can promote the accumulation of GAs in plant nodes and suppress the expression of BRC1, hence increasing primary cauline branching.

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Lynch, H. T., C. Snyder, J. Lynch, S. Ghate, and S. Thome. "Family information service (FIS) in a BRCA1 extended family." Journal of Clinical Oncology 24, no. 18_suppl (June 20, 2006): 1028. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.1028.

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1028 Background: Considering that 10% of breast cancer is hereditary, it is essential to effectively educate hereditary cancer family members regarding the syndrome, cancer risk, genetic testing and cancer control options. Researchers at Creighton University (CU) have for many years provided family (group-oriented) information services (FISs) for hereditary cancer families. Methods: Such a session was held in collaboration with a genetic counselor in a community where many members of an extended hereditary breast-ovarian cancer family with a known BRCA1 mutation reside. Family members helped contact relatives and invited them to attend the full-day FIS at a local hospital. Informal presentations were provided by a CU geneticist/oncologist, a CU nurse counselor, and a local genetic counselor. Questions and comments were encouraged. An opportunity to provide a blood sample for genetic testing was offered. Results: Contacting relatives for the FIS identified more than 400 family members from previously unknown branches. Many attendees indicated this was their first opportunity to learn about this hereditary cancer syndrome, their risk, and recommended cancer control options. Patient-stated benefits of the FIS included increased communication of cancer risk among family members as well as with their physicians, motivation to become more vigilant with screening and health management and receipt of initial cancer risk education in a non-threatening venue. Conclusions: The FIS is a relatively simple, cost-effective educational cancer control program that increases communication among family members and their physicians, educates high-risk individuals and provides an opportunity for genetic testing which will ultimately provide an accurate cancer risk status and thereby alter health management and cancer control strategies. No significant financial relationships to disclose.

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Aoyagi-Scharber, Mika, Anna S. Gardberg, Bryan K. Yip, Bing Wang, Yuqiao Shen, and Paul A. Fitzpatrick. "Structural basis for the inhibition of poly(ADP-ribose) polymerases 1 and 2 by BMN 673, a potent inhibitor derived from dihydropyridophthalazinone." Acta Crystallographica Section F Structural Biology Communications 70, no. 9 (August 29, 2014): 1143–49. http://dx.doi.org/10.1107/s2053230x14015088.

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Poly(ADP-ribose) polymerases 1 and 2 (PARP1 and PARP2), which are involved in DNA damage response, are targets of anticancer therapeutics. BMN 673 is a novel PARP1/2 inhibitor with substantially increased PARP-mediated tumor cytotoxicity and is now in later-stage clinical development for BRCA-deficient breast cancers. In co-crystal structures, BMN 673 is anchored to the nicotinamide-binding pocketviaan extensive network of hydrogen-bonding and π-stacking interactions, including those mediated by active-site water molecules. The novel di-branched scaffold of BMN 673 extends the binding interactions towards the outer edges of the pocket, which exhibit the least sequence homology among PARP enzymes. The crystallographic structural analyses reported here therefore not only provide critical insights into the molecular basis for the exceptionally high potency of the clinical development candidate BMN 673, but also new opportunities for increasing inhibitor selectivity.

14

Borger, Pieter, Anton Buzdin, Maksim Sorokin, Ekaterina Kachaylo, Bostjan Humar, Rolf Graf, and Pierre-Alien Clavien. "Large-Scale Profiling of Signaling Pathways Reveals a Distinct Demarcation between Normal and Extended Liver Resection." Cells 9, no. 5 (May 7, 2020): 1149. http://dx.doi.org/10.3390/cells9051149.

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Despite numerous studies addressing normal liver regeneration, we still lack comprehensive understanding of the biological processes underlying failed liver regeneration. Therefore, we analyzed the activity of 271 intracellular signaling pathways (ISPs) by genome wide profiling of differentially expressed RNAs in murine liver tissue biopsies after normal hepatectomy (nHx; 68% of liver removed) and extended hepatectomy (eHx; 86% of liver removed). Comprehensive, genome-wide transcriptome profiling using RNAseq was performed in liver tissue obtained from mice (sham, nHx, and eHx) harvested 1, 8, 16, 32, and 48 h after operation (n = 3 per group) and the OncoFinder toolkit was used for an unsupervised, unbiased identification of intracellular signaling pathways (ISP) activity. We observed that the normal regenerative process requires a transient activation and silencing of approximately two dozen of ISPs. After nHx, the Akt Pathway represented with 13 branches, the Chromatin Pathway and the DDR Pathways dominated. After eHx, the ATM main pathway and two of its branches (Cell Survival; G2_M Checkpoint Arrest) dominated, as well as the Hypoxia Pathways. Further, 14 ISPs demonstrated a strong inverse regulation, with the Hedgehog and the Brca1 Main Pathways as chief activators after nHx, and the ATM Pathway(G2_M Checkpoint Arrest) as the dominating constraining response after eHx.

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Zhou, Q., J. He, H. Yang, X. Luo, J. Xu, and L. Ren-Heidenreich. "RNA expression profiling of ERCC1, BRCA1, TUBB3, and STMN1 in non-small cell lung cancer by a multiplex branched DNA liquidchip technology (MBL) for predicting the efficacy of chemotherapy." Journal of Clinical Oncology 28, no. 15_suppl (May 20, 2010): e21020-e21020. http://dx.doi.org/10.1200/jco.2010.28.15_suppl.e21020.

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Li, Guofang, Ming Tan, Juanjuan Ma, Fang Cheng, Ke Li, Xiaojie Liu, Caiping Zhao, et al. "Molecular mechanism of MdWUS2–MdTCP12 interaction in mediating cytokinin signaling to control axillary bud outgrowth." Journal of Experimental Botany, June 11, 2021. http://dx.doi.org/10.1093/jxb/erab163.

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Abstract Shoot branching is an important factor that influences the architecture of apple trees and cytokinin is known to promote axillary bud outgrowth. The cultivar ‘Fuji’, which is grown on ~75% of the apple-producing area in China, exhibits poor natural branching. The TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) family genes BRANCHED1/2 (BRC1/2) are involved in integrating diverse factors that function locally to inhibit shoot branching; however, the molecular mechanism underlying the cytokinin-mediated promotion of branching that involves the repression of BRC1/2 remains unclear. In this study, we found that apple WUSCHEL2 (MdWUS2), which interacts with the co-repressor TOPLESS-RELATED9 (MdTPR9), is activated by cytokinin and regulates branching by inhibiting the activity of MdTCP12 (a BRC2 homolog). Overexpressing MdWUS2 in Arabidopsis or Nicotiana benthamiana resulted in enhanced branching. Overexpression of MdTCP12 inhibited axillary bud outgrowth in Arabidopsis, indicating that it contributes to the regulation of branching. In addition, we found that MdWUS2 interacted with MdTCP12 in vivo and in vitro and suppressed the ability of MdTCP12 to activate the transcription of its target gene, HOMEOBOX PROTEIN 53b (MdHB53b). Our results therefore suggest that MdWUS2 is involved in the cytokinin-mediated inhibition of MdTCP12 that controls bud outgrowth, and hence provide new insights into the regulation of shoot branching by cytokinin.

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Gramzow, Lydia, Katharina Klupsch, Noé Fernández-Pozo, Martin Hölzer, Manja Marz, Stefan A. Rensing, and Günter Theißen. "Comparative transcriptomics identifies candidate genes involved in the evolutionary transition from dehiscent to indehiscent fruits in Lepidium (Brassicaceae)." BMC Plant Biology 22, no. 1 (July 14, 2022). http://dx.doi.org/10.1186/s12870-022-03631-8.

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Abstract Background Fruits are the seed-bearing structures of flowering plants and are highly diverse in terms of morphology, texture and maturation. Dehiscent fruits split open upon maturation to discharge their seeds while indehiscent fruits are dispersed as a whole. Indehiscent fruits evolved from dehiscent fruits several times independently in the crucifer family (Brassicaceae). The fruits of Lepidium appelianum, for example, are indehiscent while the fruits of the closely related L. campestre are dehiscent. Here, we investigate the molecular and genetic mechanisms underlying the evolutionary transition from dehiscent to indehiscent fruits using these two Lepidium species as model system. Results We have sequenced the transcriptomes and small RNAs of floral buds, flowers and fruits of L. appelianum and L. campestre and analyzed differentially expressed genes (DEGs) and differently differentially expressed genes (DDEGs). DEGs are genes that show significantly different transcript levels in the same structures (buds, flowers and fruits) in different species, or in different structures in the same species. DDEGs are genes for which the change in expression level between two structures is significantly different in one species than in the other. Comparing the two species, the highest number of DEGs was found in flowers, followed by fruits and floral buds while the highest number of DDEGs was found in fruits versus flowers followed by flowers versus floral buds. Several gene ontology terms related to cell wall synthesis and degradation were overrepresented in different sets of DEGs highlighting the importance of these processes for fruit opening. Furthermore, the fruit valve identity genes FRUITFULL and YABBY3 were among the DEGs identified. Finally, the microRNA miR166 as well as the TCP transcription factors BRANCHED1 (BRC1) and TCP FAMILY TRANSCRIPTION FACTOR 4 (TCP4) were found to be DDEGs. Conclusions Our study reveals differences in gene expression between dehiscent and indehiscent fruits and uncovers miR166, BRC1 and TCP4 as candidate genes for the evolutionary transition from dehiscent to indehiscent fruits in Lepidium.

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Feng, Junjie, Qinlin Deng, Huanhuan Lu, Dayong Wei, Zhimin Wang, and Qinglin Tang. "Brassica juncea BRC1-1 induced by SD negatively regulates flowering by directly interacting with BjuFT and BjuFUL promoter." Frontiers in Plant Science 13 (September 30, 2022). http://dx.doi.org/10.3389/fpls.2022.986811.

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Flowering is crucial for sexual reproductive success in angiosperms. The core regulatory factors, such as FT, FUL, and SOC1, are responsible for promoting flowering. BRANCHED 1 (BRC1) is a TCP transcription factor gene that plays an important role in the regulation of branching and flowering in diverse plant species. However, the functions of BjuBRC1 in Brassica juncea are largely unknown. In this study, four homologs of BjuBRC1 were identified and the mechanism by which BjuBRC1 may function in the regulation of flowering time was investigated. Amino acid sequence analysis showed that BjuBRC1 contained a conserved TCP domain with two nuclear localization signals. A subcellular localization assay verified the nuclear localization of BjuBRC1. Expression analysis revealed that BjuBRC1-1 was induced by short days and was expressed abundantly in the leaf, flower, and floral bud but not in the root and stem in B. juncea. Overexpression of BjuBRC1-1 in the Arabidopsis brc1 mutant showed that BjuBRC1-1 delayed flowering time. Bimolecular fluorescent complementary and luciferase complementation assays showed that four BjuBRC1 proteins could interact with BjuFT in vivo. Notably, BjuBRC1 proteins formed heterodimers in vivo that may impact on their function of negatively regulating flowering time. Yeast one-hybrid, dual-luciferase reporter, and luciferase activity assays showed that BjuBRC1-1 could directly bind to the promoter of BjuFUL, but not BjuFT or BjuSOC1, to repress its expression. These results were supported by the reduced expression of AtFUL in transgenic Arabidopsis overexpressing BjuBRC1-1. Taken together, the results indicate that BjuBRC1 genes likely have a conserved function in the negative regulation of flowering in B. juncea.

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Digennaro, M., D. Sambiasi, S. Tommasi, B. Pilato, S. Diotaiuti, A. Kardhashi, G. Trojano, A. Tufaro, and A. V. Paradiso. "Hereditary and non-hereditary branches of family eligible for BRCA test: cancers in other sites." Hereditary Cancer in Clinical Practice 15, no. 1 (May 25, 2017). http://dx.doi.org/10.1186/s13053-017-0067-8.

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Wang, Xiaobei, Qiuping Wang, Lixia Yan, Yuhang Hao, Xiaodong Lian, Haipeng Zhang, Xianbo Zheng, et al. "PpTCP18 is upregulated by lncRNA5 and controls branch number in peach (Prunus persica) through positive feedback regulation of strigolactone biosynthesis." Horticulture Research, October 7, 2022. http://dx.doi.org/10.1093/hr/uhac224.

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Abstract Branch number is an important agronomic trait in peach (Prunus persica) trees because plant architecture affects fruit yield and quality. Although breeders can select varieties with different tree architecture, the biological mechanisms underlying architecture remain largely unclear. In this study, a pillar peach (‘Zhaoshouhong’) and a standard peach (‘Okubo’) were compared. ‘Zhaoshouhong’ was found to have significantly fewer secondary branches than ‘Okubo’. Treatment with the synthetic strigolactone (SL) GR24 decreased branch number. Transcriptome analysis indicated that PpTCP18 (a homologous gene of the Arabidopsis thaliana BRC1) expression was negatively correlated with strigolactone synthesis gene expression, indicating that PpTCP18 may play an important role in peach branching. Yeast one-hybrid, electrophoretic mobility shift, dual-luciferase assays and PpTCP18-knockdown in peach leaf buds indicated that PpTCP18 could increase expression of PpLBO1, PpMAX1, and PpMAX4. Furthermore, transgenic Arabidopsis plants overexpressing PpTCP18 clearly exhibited a reduced primary rosette-leaf branches. Moreover, lncRNA sequencing and transient expression analysis revealed that lncRNA5 targeted PpTCP18, significantly increasing PpTCP18 expression. These results provide insights into the mRNA and lncRNA network in the peach SL signaling pathway and indicate that PpTCP18, a transcription factor downstream of SL signaling, was involved in positive feedback regulation of SL biosynthesis. This role of PpTCP18 may represent a novel mechanism in peach branching regulation. Our study improves current understanding of the mechanisms underlying peach branching and provides theoretical support for genetic improvement of peach tree architecture.

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Ordonez, Liliana D., Lorenzo Melchor, Kirsty R. Greenow, Howard Kendrick, Giusy Tornillo, James Bradford, Peter Giles, and Matthew J. Smalley. "Reproductive history determines Erb b 2 locus amplification, WNT signalling and tumour phenotype in a murine breast cancer model." Disease Models & Mechanisms 14, no. 5 (May 1, 2021). http://dx.doi.org/10.1242/dmm.048736.

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ABSTRACT Understanding the mechanisms underlying tumour heterogeneity is key to the development of treatments that can target specific tumour subtypes. We have previously targeted CRE recombinase-dependent conditional deletion of the tumour suppressor genes Brca1, Brca2, p53 (also known as Trp53) and/or Pten to basal or luminal oestrogen receptor-negative (ER−) cells of the mouse mammary epithelium. We demonstrated that both the cell-of-origin and the tumour-initiating genetic lesions cooperate to influence mammary tumour phenotype. Here, we use a CRE-activated HER2 orthologue to specifically target HER2/ERBB2 oncogenic activity to basal or luminal ER− mammary epithelial cells and perform a detailed analysis of the tumours that develop. We find that, in contrast to our previous studies, basal epithelial cells are less sensitive to transformation by the activated NeuKI allele, with mammary epithelial tumour formation largely confined to luminal ER− cells. Histologically, most tumours that developed were classified as either adenocarcinomas of no special type or as metaplastic adenosquamous tumours. The former were typically characterized by amplification of the NeuNT/Erbb2 locus; in contrast, tumours displaying squamous metaplasia were enriched in animals that had been through at least one pregnancy and typically had lower levels of NeuNT/Erbb2 locus amplification but had activated canonical WNT signalling. Squamous changes in these tumours were associated with activation of the epidermal differentiation cluster. Thus, in this model of HER2 breast cancer, cell-of-origin, reproductive history, NeuNT/Erbb2 locus amplification and the activation of specific branches of the WNT signalling pathway all interact to drive inter-tumour heterogeneity.

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