Добірка наукової літератури з теми "Buffalo preimplantation embryos"

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Статті в журналах з теми "Buffalo preimplantation embryos"

1

Eswari, S., G. Sai Kumar та G. Taru Sharma. "Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation". Zygote 21, № 2 (2012): 203–13. http://dx.doi.org/10.1017/s0967199412000172.

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SummaryThe objective of this study was to evaluate the effect of supplementation of recombinant leukaemia inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor (LIFRβ) genes in different stages of preimplantation buffalo embryos. Cumulus–oocyte complexes retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo s
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2

Karaivanov, C., K. Vlahov, M. Petrov, et al. "Studies on preimplantation development of buffalo embryos." Theriogenology 28, no. 5 (1987): 747–53. http://dx.doi.org/10.1016/0093-691x(87)90291-3.

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3

SHARMA, A. K., GOPAL PURI, V. B. KHARADI, and S. K. BHAVSAR. "In vitro production of early stage buffalo embryos in modified synthetic oviductal fluid (mSOF) medium." Indian Journal of Animal Sciences 88, no. 2 (2018): 176–80. http://dx.doi.org/10.56093/ijans.v88i2.79318.

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The objective of the present study was to observe the developmental rates and the stage of development since fertilization of in vitro produced early stage buffalo embryos. Buffalo cumulus-oocyte complexes (COC’s) obtained from slaughterhouse ovaries were matured and fertilized in vitro. The fertilized oocytes (400) were then cultured in modified synthetic oviductal fluid (mSOF) medium containing bovine serum albumin (BSA) and fetal bovine serum (FBS) and evaluated for the developmental stages of preimplantation early stage embryos up to morula on 48 h, 72 h, 96 h and 7th day post fertilizatio
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4

Kitiyanant, Y., J. Saikhun, N. Kitiyanant, H. Sritanaudomchai, T. Faisaikarm, and K. Pavasuthipaisit. "189ESTABLISHMENT OF BUFFALO EMBRYONIC STEM-LIKE (ES-LIKE) CELL LINES FROM DIFFERENT SOURCES OF DERIVED BLASTOCYSTS." Reproduction, Fertility and Development 16, no. 2 (2004): 216. http://dx.doi.org/10.1071/rdv16n1ab189.

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In the pleuripotent embryonic stem (ES) cells in mammalian species are derived from the inner cell mass (ICM ) of preimplantation embryos. In the current study we report the successful isolation of pleuripotent undifferentiated buffalo ES-like cells from the ICMs of in vitro fertilization (IVF), somatic cell nuclear transfer (NT)-reconstructed and parthenogenetic (PA) embryos. The ICMs were isolated from hatched blastocysts that had spread out after 3–5 days of culture on mouse embryonic fibroblast (MEF) feeder cell layer in the presence of leukemia inhibiting factor (LIF). The production of M
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5

Simon, Liz, C. Veerapandian, S. Balasubramanian, and A. Subramanian. "Somatic cell nuclear transfer in buffalos: effect of the fusion and activation protocols and embryo culture system on preimplantation embryo development." Reproduction, Fertility and Development 18, no. 4 (2006): 439. http://dx.doi.org/10.1071/rd05079.

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The present study was conducted primarily to evaluate several factors that affect the nuclear transfer programme in water buffalos, in which relatively few studies have been performed. Embryos reconstructed with quiescent fetal fibroblasts and metaphase II cytoplasts were matured for 24 h, and activation was found to be comparatively better than in those matured for 30 h. A significantly higher proportion of embryos fused (52.0 ± 1.9) and cleaved (51.2 ± 1.7) when the couplets were fused 4–6 h before activation than when fused and activated simultaneously (46.5 ± 1.6 and 44.5 ± 2.0, respective
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6

Verma, A., P. Kumar, S. Rajput, B. Roy, S. De, and T. K. Datta. "Embryonic genome activation events in buffalo (Bubalus bubalis) preimplantation embryos." Molecular Reproduction and Development 79, no. 5 (2012): 321–28. http://dx.doi.org/10.1002/mrd.22027.

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7

Edwards, J. L., A. M. Powell, and C. E. Rexroad Jr. "Alkaline phosphatase activity in bovine oocytes and preimplantation embryos as affected by removal of the zona pellucida and culture medium constituents." Reproduction, Fertility and Development 15, no. 5 (2003): 285. http://dx.doi.org/10.1071/rd03025.

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The aims of the present study were: (1) to characterize alkaline phosphatase (AP) activity in bovine oocytes and embryos with intact or removed zona pellucida (ZP); and (2) to evaluate the effect of culture medium constituents on AP activity. Alkaline phosphatase activity in non-matured and matured oocytes was most evident nearest the plasma membrane and perivitelline space. In more than 90% of two- to 16-cell embryos, AP activity was observed in the perivitelline space and at blastomere contacts. In blastocysts, AP activity was localized to the trophectoderm. Only after immunodissection was A
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8

Huang, Gao-Bo, Li Quan, Yong-Lian Zeng, Jian Yang, Ke-Huan Lu, and Sheng-Sheng Lu. "Role of linker histone H1c during the reprogramming of Chinese swamp buffalo (Bubalus Bubalis) embryos produced by somatic cell nuclear transfer." Reproduction, Fertility and Development 28, no. 3 (2016): 302. http://dx.doi.org/10.1071/rd14051.

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During reprogramming, there is exchange of histone H1c and the oocyte-specific linker histone, and H1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (SCNT). The aim of the present study was to investigate the role of the H1c gene in SCNT reprogramming in Chinese swamp buffalo (Bubalus bubalis) using RNA interference (RNAi). Chinese swamp buffalo H1c gene sequences were obtained and H1c-RNAi vectors were designed, synthesised and then transfected into a buffalo fetal skin fibroblast cell line. Expression of H1c was determined by real-time pol
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9

K, NAHEEF, BRINDHA K, SENTHIL KUMAR T. M A, SESH P. S L, PARTHIBAN M, and RANGASAMY S. "Isolation and characterization of exosomes derived from buffalo oviductal epithelial cells." Indian Journal of Animal Sciences 94, no. 9 (2024): 770–73. http://dx.doi.org/10.56093/ijans.v94i9.148385.

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In the present study, exosomes were successfully isolated from the conditioned medium of buffalo OECs cultured in vitro. Characterisation by nanoparticle analysis revealed that the size of the oviductal exosomes ranged between 40-150 nm. Imaging by Transmission electron microscopy showed that these exosomes exhibited circular/spherical morphology. The identity of isolated exosomes was further confirmed by analysing the expression of surface expressed tetraspannin markers (CD9, CD63, and CD81) by flow cytometry, and were found to be enriched with the tetraspannins analysed. As continuation of t
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10

Lu, F., T. Luo, H. Sun, et al. "136 EFFECTS OF INSULIN-LIKE GROWTH FACTOR I (IGF-1) ON THE DEVELOPMENT AND APOPTOSIS OF PREIMPLANTATION BUFFALO (BUBALUS BUBALIS) EMBRYOS." Reproduction, Fertility and Development 25, no. 1 (2013): 215. http://dx.doi.org/10.1071/rdv25n1ab136.

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The aim of this study was to explore the effects of insulin-like growth factor-I (IGF-1) on the development and apoptosis of preimplantation buffalo (Bubalus bubalis) embryos derived from IVF or somatic cell nuclear transfer (SCNT) in order to improve the quality of in vitro embryo culture (IVC). Buffalo oocytes collected from ovaries at slaughter were cultured in the maturation medium (TCM-199 + 26.2 mmol L–1 NaHCO3 + 5 mmol L–1 HEPES + 5% FBS) for 22–24 h, and fertilized in vitro, or enucleated and reconstructed for SCNT. Embryos were then cultured in the culture medium (CM: TCM-199 + 3% FBS
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