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1

Eswari, S., G. Sai Kumar та G. Taru Sharma. "Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation". Zygote 21, № 2 (2012): 203–13. http://dx.doi.org/10.1017/s0967199412000172.

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SummaryThe objective of this study was to evaluate the effect of supplementation of recombinant leukaemia inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor (LIFRβ) genes in different stages of preimplantation buffalo embryos. Cumulus–oocyte complexes retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo s
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2

Karaivanov, C., K. Vlahov, M. Petrov, et al. "Studies on preimplantation development of buffalo embryos." Theriogenology 28, no. 5 (1987): 747–53. http://dx.doi.org/10.1016/0093-691x(87)90291-3.

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3

SHARMA, A. K., GOPAL PURI, V. B. KHARADI, and S. K. BHAVSAR. "In vitro production of early stage buffalo embryos in modified synthetic oviductal fluid (mSOF) medium." Indian Journal of Animal Sciences 88, no. 2 (2018): 176–80. http://dx.doi.org/10.56093/ijans.v88i2.79318.

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The objective of the present study was to observe the developmental rates and the stage of development since fertilization of in vitro produced early stage buffalo embryos. Buffalo cumulus-oocyte complexes (COC’s) obtained from slaughterhouse ovaries were matured and fertilized in vitro. The fertilized oocytes (400) were then cultured in modified synthetic oviductal fluid (mSOF) medium containing bovine serum albumin (BSA) and fetal bovine serum (FBS) and evaluated for the developmental stages of preimplantation early stage embryos up to morula on 48 h, 72 h, 96 h and 7th day post fertilizatio
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4

Kitiyanant, Y., J. Saikhun, N. Kitiyanant, H. Sritanaudomchai, T. Faisaikarm, and K. Pavasuthipaisit. "189ESTABLISHMENT OF BUFFALO EMBRYONIC STEM-LIKE (ES-LIKE) CELL LINES FROM DIFFERENT SOURCES OF DERIVED BLASTOCYSTS." Reproduction, Fertility and Development 16, no. 2 (2004): 216. http://dx.doi.org/10.1071/rdv16n1ab189.

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In the pleuripotent embryonic stem (ES) cells in mammalian species are derived from the inner cell mass (ICM ) of preimplantation embryos. In the current study we report the successful isolation of pleuripotent undifferentiated buffalo ES-like cells from the ICMs of in vitro fertilization (IVF), somatic cell nuclear transfer (NT)-reconstructed and parthenogenetic (PA) embryos. The ICMs were isolated from hatched blastocysts that had spread out after 3–5 days of culture on mouse embryonic fibroblast (MEF) feeder cell layer in the presence of leukemia inhibiting factor (LIF). The production of M
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5

Simon, Liz, C. Veerapandian, S. Balasubramanian, and A. Subramanian. "Somatic cell nuclear transfer in buffalos: effect of the fusion and activation protocols and embryo culture system on preimplantation embryo development." Reproduction, Fertility and Development 18, no. 4 (2006): 439. http://dx.doi.org/10.1071/rd05079.

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The present study was conducted primarily to evaluate several factors that affect the nuclear transfer programme in water buffalos, in which relatively few studies have been performed. Embryos reconstructed with quiescent fetal fibroblasts and metaphase II cytoplasts were matured for 24 h, and activation was found to be comparatively better than in those matured for 30 h. A significantly higher proportion of embryos fused (52.0 ± 1.9) and cleaved (51.2 ± 1.7) when the couplets were fused 4–6 h before activation than when fused and activated simultaneously (46.5 ± 1.6 and 44.5 ± 2.0, respective
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6

Verma, A., P. Kumar, S. Rajput, B. Roy, S. De, and T. K. Datta. "Embryonic genome activation events in buffalo (Bubalus bubalis) preimplantation embryos." Molecular Reproduction and Development 79, no. 5 (2012): 321–28. http://dx.doi.org/10.1002/mrd.22027.

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7

Edwards, J. L., A. M. Powell, and C. E. Rexroad Jr. "Alkaline phosphatase activity in bovine oocytes and preimplantation embryos as affected by removal of the zona pellucida and culture medium constituents." Reproduction, Fertility and Development 15, no. 5 (2003): 285. http://dx.doi.org/10.1071/rd03025.

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The aims of the present study were: (1) to characterize alkaline phosphatase (AP) activity in bovine oocytes and embryos with intact or removed zona pellucida (ZP); and (2) to evaluate the effect of culture medium constituents on AP activity. Alkaline phosphatase activity in non-matured and matured oocytes was most evident nearest the plasma membrane and perivitelline space. In more than 90% of two- to 16-cell embryos, AP activity was observed in the perivitelline space and at blastomere contacts. In blastocysts, AP activity was localized to the trophectoderm. Only after immunodissection was A
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8

Huang, Gao-Bo, Li Quan, Yong-Lian Zeng, Jian Yang, Ke-Huan Lu, and Sheng-Sheng Lu. "Role of linker histone H1c during the reprogramming of Chinese swamp buffalo (Bubalus Bubalis) embryos produced by somatic cell nuclear transfer." Reproduction, Fertility and Development 28, no. 3 (2016): 302. http://dx.doi.org/10.1071/rd14051.

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During reprogramming, there is exchange of histone H1c and the oocyte-specific linker histone, and H1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (SCNT). The aim of the present study was to investigate the role of the H1c gene in SCNT reprogramming in Chinese swamp buffalo (Bubalus bubalis) using RNA interference (RNAi). Chinese swamp buffalo H1c gene sequences were obtained and H1c-RNAi vectors were designed, synthesised and then transfected into a buffalo fetal skin fibroblast cell line. Expression of H1c was determined by real-time pol
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9

K, NAHEEF, BRINDHA K, SENTHIL KUMAR T. M A, SESH P. S L, PARTHIBAN M, and RANGASAMY S. "Isolation and characterization of exosomes derived from buffalo oviductal epithelial cells." Indian Journal of Animal Sciences 94, no. 9 (2024): 770–73. http://dx.doi.org/10.56093/ijans.v94i9.148385.

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In the present study, exosomes were successfully isolated from the conditioned medium of buffalo OECs cultured in vitro. Characterisation by nanoparticle analysis revealed that the size of the oviductal exosomes ranged between 40-150 nm. Imaging by Transmission electron microscopy showed that these exosomes exhibited circular/spherical morphology. The identity of isolated exosomes was further confirmed by analysing the expression of surface expressed tetraspannin markers (CD9, CD63, and CD81) by flow cytometry, and were found to be enriched with the tetraspannins analysed. As continuation of t
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10

Lu, F., T. Luo, H. Sun, et al. "136 EFFECTS OF INSULIN-LIKE GROWTH FACTOR I (IGF-1) ON THE DEVELOPMENT AND APOPTOSIS OF PREIMPLANTATION BUFFALO (BUBALUS BUBALIS) EMBRYOS." Reproduction, Fertility and Development 25, no. 1 (2013): 215. http://dx.doi.org/10.1071/rdv25n1ab136.

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The aim of this study was to explore the effects of insulin-like growth factor-I (IGF-1) on the development and apoptosis of preimplantation buffalo (Bubalus bubalis) embryos derived from IVF or somatic cell nuclear transfer (SCNT) in order to improve the quality of in vitro embryo culture (IVC). Buffalo oocytes collected from ovaries at slaughter were cultured in the maturation medium (TCM-199 + 26.2 mmol L–1 NaHCO3 + 5 mmol L–1 HEPES + 5% FBS) for 22–24 h, and fertilized in vitro, or enucleated and reconstructed for SCNT. Embryos were then cultured in the culture medium (CM: TCM-199 + 3% FBS
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11

Thiyagarajan, B., and K. Valivittan. "Ameliorating effect of vitamin E on in vitro development of preimplantation buffalo embryos." Journal of Assisted Reproduction and Genetics 26, no. 4 (2009): 217–25. http://dx.doi.org/10.1007/s10815-009-9302-1.

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12

Thiyagarajan, B., and K. Valivittan. "Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos." Animal 3, no. 3 (2009): 385–92. http://dx.doi.org/10.1017/s1751731108003455.

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13

Suárez Novoa, M. V., S. Di Francesco, M. Rubessa, et al. "126 EFFECT OF REDUCING GLUCOSE CONCENTRATION DURING IN VITRO EMBRYO CULTURE IN BUFFALO (BUBALUS BUBALIS)." Reproduction, Fertility and Development 23, no. 1 (2011): 168. http://dx.doi.org/10.1071/rdv23n1ab126.

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The current knowledge on metabolism and glucose utilisation of preimplantation bovine and ovine embryos suggest the reduction of glucose concentration during early culture. On the contrary, it has been demonstrated that glucose is absolutely required for in vitro culture of buffalo embryos, as indicated by the poor efficiency recorded in the absence of this substrate during early embryonic development (Monaco et al. 2006 Reprod. Domest. Anim. 41, 332). However, complete removal of glucose from culture medium throughout pre-elongation development is unlikely to benefit the embryo because glucos
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14

Matshikiza, M., P. Bartels, G. Vajta, et al. "57EMBRYO DEVELOPMENT FOLLOWING INTERSPECIES NUCLEAR TRANSFER OF AFRICAN BUFFALO (SYNCERUS CAFFER), BONTEBOK (DAMALISCUS DORCUS DORCUS) AND ELAND (TAUROTRAGUS ORYX) SOMATIC CELLS INTO BOVINE CYTOPLASTS." Reproduction, Fertility and Development 16, no. 2 (2004): 150. http://dx.doi.org/10.1071/rdv16n1ab57.

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Wildlife conservation requires traditional as well as innovative conservation strategies in order to preserve gene and species diversity. Interspecies nuclear transfer has the potential to conserve genes from critically endangered wildlife species where few or no oocytes are available from the endangered species, and where representative cell lines have been established for the wildlife population while numbers were still abundant. The purpose of this study was to investigate the developmental ability of embryos reconstructed with transfer of somatic cells from the African buffalo (Syncerus ca
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15

Kumar, D., T. Anand, K. P. Singh, M. S. Chauhan, P. Palta, and R. S. Manik. "165 EXPRESSION OF Oct-4 IN BUFFALO (BUBALUS BUBALIS) EMBRYOS GENERATED THROUGH IN VITRO FERTILIZATION OR PARTHENOGENETIC ACTIVATION." Reproduction, Fertility and Development 20, no. 1 (2008): 162. http://dx.doi.org/10.1071/rdv20n1ab165.

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Octamer-4 (Oct-4) is a member of Class V of the POU transcription factors family, which is involved in transcriptional regulation during early embryonic development and cell differentiation. It is expressed in the inner cell mass of blastocysts and in embryonic stem cells (ESCs), and its expression is widely used as a marker of pluripotency in ESCs in many species. This study was, therefore, carried out to examine the expression of Oct-4 in embryos at the 2-, 4-, 8- to 16-cell, morula, and blastocyst stages generated through IVF or parthenogenetic activation. A total of 100 embryos were used i
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16

Sadeesh, E. M., N. L. Selokar, A. K. Balhara, and P. S. Yadav. "Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media." Cytotechnology 68, no. 5 (2016): 1973–86. http://dx.doi.org/10.1007/s10616-016-0010-2.

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17

Pandey, A., S. C. Gupta, and N. Gupta. "192 EFFECT OF DIFFERENT CULTURE MEDIUM ON THE PRODUCTION OF GRANULOSA SCNT EMBRYOS IN WATER BUFFALO (BUBALUS BUBALIS) AND THEIR mRNA TRANSCRIPT ANALYSIS AT DEVELOPMENTAL STAGES IN COMPARISON WITH IN VITRO FERTILIZATION-PRODUCED EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 194. http://dx.doi.org/10.1071/rdv21n1ab192.

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Success of NT is as low as 0.2 to 3.4% in cloned offspring, and many cloned embryos arrest development before implantation. Expression patterns of mRNA in NT embryos at critical time points play an important role in epigenetic reprogramming and are affected by external factors (Dean et al. 2001 PNAS 98, 13 734–13 738). Embryo culture system can affect the silencing or activations of a particular gene at fetal development stages (Wrenzycki et al. 2001 Hum. Reprod. 16, 893–901). The study was aimed to analyze the expression of genes responsible for growth and embryogenesis (IGF1, IGF2, IGF1R &am
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18

Anand, T., D. Kumar, MK Singh, et al. "Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells and Preimplantation Embryos Exhibit Comparable Expression of Pluripotency-Related Antigens." Reproduction in Domestic Animals 46, no. 1 (2011): 50–58. http://dx.doi.org/10.1111/j.1439-0531.2009.01564.x.

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19

Wang, K., Z. Beyhan, R. Rodriguez, et al. "61 BOVINE OOPLASM PARTIALLY REMODELS CHIMPANZEE SOMATIC NUCLEI FOLLOWING SOMATIC CELL NUCLEAR TRANSFER." Reproduction, Fertility and Development 20, no. 1 (2008): 111. http://dx.doi.org/10.1071/rdv20n1ab61.

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Interspecies somatic cell nuclear transfer (iSCNT) is a tool to address basic questions related to nucleus–cytoplasm interactions between different species. This approach may have practical applications, such as endangered species preservation. To date, live offspring have been obtained only when closely related species were used, such as cow/gaur, cow/buffalo, and domestic cat/wild cat. Several groups have reported preimplantation development of iSCNT embryos using a diverse collection of species; however, further development of these embryos either failed or was not reported. The aim of this
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20

Daliri, M., K. B. C. Appa Rao, G. Kaur, S. Garg, S. Patil, and S. M. Totey. "Expression of growth factor ligand and receptor genes in preimplantation stage water buffalo (Bubalus bubalis) embryos and oviduct epithelial cells." Reproduction 117, no. 1 (1999): 61–70. http://dx.doi.org/10.1530/jrf.0.1170061.

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21

Taru Sharma, Gutulla, Amar Nath, Shiv Prasad, Sumit Singhal, Vikash Chandra, and Gutulla Saikumar. "Expression pattern of GLUT 1, 5, 8 and citrate synthase transcripts in buffalo ( Bubalus bubalis ) preimplantation embryos produced in vitro and derived in vivo." Reproduction in Domestic Animals 55, no. 10 (2020): 1362–70. http://dx.doi.org/10.1111/rda.13782.

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22

Rajhans, Rajib, G. Sai Kumar, Pawan K. Dubey, and G. Taru Sharma. "Effect of timing of development on total cell number and expression profile of HSP-70.1 and GLUT-1 in buffalo (Bubalus bubalis) oocytes and preimplantation embryos producedin vitro." Cell Biology International 34, no. 5 (2010): 463–68. http://dx.doi.org/10.1042/cbi20090295.

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23

Pang, Chun-Ying, Ming-Zhou Bai, Chi Zhang, et al. "Global transcriptome analysis of different stages of preimplantation embryo development in river buffalo." PeerJ 7 (December 2, 2019): e8185. http://dx.doi.org/10.7717/peerj.8185.

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Background Water buffalo (Bubalus bubalis) are divided into river buffalo and swamp buffalo subspecies and are essential livestock for agriculture and the local economy. Studies on buffalo reproduction have primarily focused on optimal fertility and embryonic mortality. There is currently limited knowledge on buffalo embryonic development, especially during the preimplantation period. Assembly of the river buffalo genome offers a reference for omics studies and facilitates transcriptomic analysis of preimplantation embryo development (PED). Methods We revealed transcriptomic profile of four st
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24

Figueiró, Marivaldo Rodrigues, Joaquim Mansano Garcia, Marina Ragagnin de Lima, Maite del Collado, and Naiara Zoccal Saraiva. "Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?" Zygote 28, no. 2 (2020): 109–15. http://dx.doi.org/10.1017/s096719941900073x.

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SummaryIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest
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25

Misra, A. K., R. Kasiraj, M. Mutha Rao, N. S. Rangareddy, R. S. Jaiswal, and H. C. Pant. "Rate of transport and development of preimplantation embryo in the superovulated buffalo ()." Theriogenology 50, no. 4 (1998): 637–49. http://dx.doi.org/10.1016/s0093-691x(98)00168-x.

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26

Zhuang, X.-J., Y. Huang, Y.-P. Duan, M. Zhang, Y.-Q. Lu, and K.-H. Lu. "Translocation of Active Mitochondria During Buffalo (Bubalus bubalis) Oocytes In vitro Maturation, Fertilization and Preimplantation Embryo Development." Reproduction in Domestic Animals 47, no. 3 (2011): 443–48. http://dx.doi.org/10.1111/j.1439-0531.2011.01900.x.

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27

Pandey, Alok, S. C. Gupta, and Neelam Gupta. "Comparative Potential of Cultured Skin Fibroblast, Cumulus, and Granulosa Cell to Produce Somatic Cell Nuclear Transfer (SCNT) Preimplantation Embryos in Buffaloes (Bubalus bubalis) in Relation to Gene Expressions." Cellular Reprogramming 12, no. 3 (2010): 357–68. http://dx.doi.org/10.1089/cell.2009.0083.

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28

Sun, Qinqiang, Yuan Yang, Yue Zhang, et al. "Padi6 expression patterns in buffalo oocytes and preimplantation embryos." Animal Reproduction 21, no. 1 (2024). http://dx.doi.org/10.1590/1984-3143-ar2023-0146.

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29

Abu-El, Naga EM1 El-Sheikh Ali H2 Balboula AZ2 Ramdan Badr M3 Abd El-Moneim M2 Said Hussein M4 Abd El-Raouf OH2 5. Zaabel SM. "Effect of Enrichment of In Vitro Fertilization Medium with Cysteine on Fertilization and Embryo Development in Buffaloes." July 13, 2017. https://doi.org/10.19070/2332-2748-1700039.

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The current study aimed to elucidate the effect of adding various concentrations of cysteine to the in vitro fertilization culture medium on the fertilization rate and following embryonic development of buffalo oocytes. In vitro matured oocytes were fertilized using different concentrations of cysteine (0.5, 1.3 mM) in the presence of 5.0 mM caffeine. After in vitro fertilization, fertilization rate, cleavage rate and developmental rates till the blastocyst stage have been evaluated. The present results revealed that supplementation of the fertilization media with 0.3 Mm cysteine enhanced sign
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30

Malpotra, Shivani, Pallavi Goel, Songyukta Shyam, Manoj Kumar Singh, and Prabhat Palta. "Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization." Scientific Reports 12, no. 1 (2022). http://dx.doi.org/10.1038/s41598-022-09207-8.

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AbstractSomatic cell nuclear transfer technique (SCNT) has proved to be an outstanding method of multiplication of elite animals but accompanied with low efficiency and live birth rate of cloned animals. Epigenetic alterations of DNA has been one of the culprits behind this issue. Cloned embryos are found to deviate slightly from regular pattern of demethylation and re-methylation at the time of nuclear reprogramming and embryonic development when compared with embryos produced by in vitro fertilization (IVF). Thus, the present study was aimed at evaluating global DNA methylation profiles of c
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31

"Transport of Preimplantation Embryos in the Genitalia of Buffalo Heifers Superovulated with pFSH and Variable Doses of LH." Assiut Veterinary Medical Journal 53, no. 114 (2007): 1–9. http://dx.doi.org/10.21608/avmj.2007.177606.

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