Добірка наукової літератури з теми "Chromatid cohesion"

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Статті в журналах з теми "Chromatid cohesion"

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Mishra, Prashant K., Sultan Ciftci-Yilmaz, David Reynolds, et al. "Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis." Molecular Biology of the Cell 27, no. 14 (2016): 2286–300. http://dx.doi.org/10.1091/mbc.e16-01-0004.

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Анотація:
Sister chromatid cohesion is essential for tension-sensing mechanisms that monitor bipolar attachment of replicated chromatids in metaphase. Cohesion is mediated by the association of cohesins along the length of sister chromatid arms. In contrast, centromeric cohesin generates intrastrand cohesion and sister centromeres, while highly cohesin enriched, are separated by >800 nm at metaphase in yeast. Removal of cohesin is necessary for sister chromatid separation during anaphase, and this is regulated by evolutionarily conserved polo-like kinase (Cdc5 in yeast, Plk1 in humans). Here we addre
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Sapkota, Hem, Emilia Wasiak, John R. Daum, and Gary J. Gorbsky. "Multiple determinants and consequences of cohesion fatigue in mammalian cells." Molecular Biology of the Cell 29, no. 15 (2018): 1811–24. http://dx.doi.org/10.1091/mbc.e18-05-0315.

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Анотація:
Cells delayed in metaphase with intact mitotic spindles undergo cohesion fatigue, where sister chromatids separate asynchronously, while cells remain in mitosis. Cohesion fatigue requires release of sister chromatid cohesion. However, the pathways that breach sister chromatid cohesion during cohesion fatigue remain unknown. Using moderate-salt buffers to remove loosely bound chromatin cohesin, we show that “cohesive” cohesin is not released during chromatid separation during cohesion fatigue. Using a regulated protein heterodimerization system to lock different cohesin ring interfaces at speci
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Shi, Di, Shuaijun Zhao, Mei-Qing Zuo, et al. "The acetyltransferase Eco1 elicits cohesin dimerization during S phase." Journal of Biological Chemistry 295, no. 22 (2020): 7554–65. http://dx.doi.org/10.1074/jbc.ra120.013102.

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Анотація:
Cohesin is a DNA-associated protein complex that forms a tripartite ring controlling sister chromatid cohesion, chromosome segregation and organization, DNA replication, and gene expression. Sister chromatid cohesion is established by the protein acetyltransferase Eco1, which acetylates two conserved lysine residues on the cohesin subunit Smc3 and thereby ensures correct chromatid separation in yeast (Saccharomyces cerevisiae) and other eukaryotes. However, the consequence of Eco1-catalyzed cohesin acetylation is unknown, and the exact nature of the cohesive state of chromatids remains controv
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Boavida, Ana, Diana Santos, Mohammad Mahtab, and Francesca M. Pisani. "Functional Coupling between DNA Replication and Sister Chromatid Cohesion Establishment." International Journal of Molecular Sciences 22, no. 6 (2021): 2810. http://dx.doi.org/10.3390/ijms22062810.

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Анотація:
Several lines of evidence suggest the existence in the eukaryotic cells of a tight, yet largely unexplored, connection between DNA replication and sister chromatid cohesion. Tethering of newly duplicated chromatids is mediated by cohesin, an evolutionarily conserved hetero-tetrameric protein complex that has a ring-like structure and is believed to encircle DNA. Cohesin is loaded onto chromatin in telophase/G1 and converted into a cohesive state during the subsequent S phase, a process known as cohesion establishment. Many studies have revealed that down-regulation of a number of DNA replicati
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Chen, Yu-Fan, Chia-Ching Chou, and Marc R. Gartenberg. "Determinants of Sir2-Mediated, Silent Chromatin Cohesion." Molecular and Cellular Biology 36, no. 15 (2016): 2039–50. http://dx.doi.org/10.1128/mcb.00057-16.

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Анотація:
Cohesin associates with distinct sites on chromosomes to mediate sister chromatid cohesion. Single cohesin complexes are thought to bind by encircling both sister chromatids in a topological embrace. Transcriptionally repressed chromosomal domains in the yeastSaccharomyces cerevisiaerepresent specialized sites of cohesion where cohesin binds silent chromatin in a Sir2-dependent fashion. In this study, we investigated the molecular basis for Sir2-mediated cohesion. We identified a cluster of charged surface residues of Sir2, collectively termed the EKDK motif, that are required for cohesin func
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Ochs, Fena, Charlotte Green, Aleksander Tomasz Szczurek, et al. "Sister chromatid cohesion is mediated by individual cohesin complexes." Science 383, no. 6687 (2024): 1122–30. http://dx.doi.org/10.1126/science.adl4606.

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Анотація:
Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin’s stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclus
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Henrikus, Sarah S., and Alessandro Costa. "Towards a Structural Mechanism for Sister Chromatid Cohesion Establishment at the Eukaryotic Replication Fork." Biology 10, no. 6 (2021): 466. http://dx.doi.org/10.3390/biology10060466.

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Анотація:
Cohesion between replicated chromosomes is essential for chromatin dynamics and equal segregation of duplicated genetic material. In the G1 phase, the ring-shaped cohesin complex is loaded onto duplex DNA, enriching at replication start sites, or “origins”. During the same phase of the cell cycle, and also at the origin sites, two MCM helicases are loaded as symmetric double hexamers around duplex DNA. During the S phase, and through the action of replication factors, cohesin switches from encircling one parental duplex DNA to topologically enclosing the two duplicated DNA filaments, which are
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Nakamura, Akito, Hiroyuki Arai, and Naoya Fujita. "Centrosomal Aki1 and cohesin function in separase-regulated centriole disengagement." Journal of Cell Biology 187, no. 5 (2009): 607–14. http://dx.doi.org/10.1083/jcb.200906019.

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Sister chromatid separation at anaphase is triggered by cleavage of the cohesin subunit Scc1, which is mediated by separase. Centriole disengagement also requires separase. This dual role of separase permits concurrent control of these events for accurate metaphase to anaphase transition. Although the molecular mechanism underlying sister chromatid cohesion has been clarified, that of centriole cohesion is poorly understood. In this study, we show that Akt kinase–interacting protein 1 (Aki1) localizes to centrosomes and regulates centriole cohesion. Aki1 depletion causes formation of multipola
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Carvalhal, Sara, Alexandra Tavares, Mariana B. Santos, Mihailo Mirkovic, and Raquel A. Oliveira. "A quantitative analysis of cohesin decay in mitotic fidelity." Journal of Cell Biology 217, no. 10 (2018): 3343–53. http://dx.doi.org/10.1083/jcb.201801111.

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Анотація:
Sister chromatid cohesion mediated by cohesin is essential for mitotic fidelity. It counteracts spindle forces to prevent premature chromatid individualization and random genome segregation. However, it is unclear what effects a partial decline of cohesin may have on chromosome organization. In this study, we provide a quantitative analysis of cohesin decay by inducing acute removal of defined amounts of cohesin from metaphase-arrested chromosomes. We demonstrate that sister chromatid cohesion is very resistant to cohesin loss as chromatid disjunction is only observed when chromosomes lose &am
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Stanyte, Rugile, Johannes Nuebler, Claudia Blaukopf, et al. "Dynamics of sister chromatid resolution during cell cycle progression." Journal of Cell Biology 217, no. 6 (2018): 1985–2004. http://dx.doi.org/10.1083/jcb.201801157.

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Анотація:
Faithful genome transmission in dividing cells requires that the two copies of each chromosome’s DNA package into separate but physically linked sister chromatids. The linkage between sister chromatids is mediated by cohesin, yet where sister chromatids are linked and how they resolve during cell cycle progression has remained unclear. In this study, we investigated sister chromatid organization in live human cells using dCas9-mEGFP labeling of endogenous genomic loci. We detected substantial sister locus separation during G2 phase irrespective of the proximity to cohesin enrichment sites. Alm
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Дисертації з теми "Chromatid cohesion"

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Liu, Zhe. "Characterization of sister chromatid cohesins having overlapping function and the role of separase, AtESP1, in controlling sister chromatid cohesion in Arabidopsis." Connect to this document online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1134155133.

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Thesis (Ph. D.)--Miami University, Dept. of Chemistry and Biochemistry, 2005.<br>Title from second page of PDF document. Document formatted into pages; contains [3], vi, 124 p. : ill. Includes bibliographical references.
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Almedawar, Seba. "A SUMO-dependent step during establishment of Sister Chromatid Cohesion." Doctoral thesis, Universitat de Lleida, 2013. http://hdl.handle.net/10803/123807.

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Анотація:
Els anells de cohesina, formats per les proteïnes Smc1, Smc3, Scc1 i Scc3, s’uneixen topològicament al DNA, mantenint les parelles de cromàtides germanes unides des de la duplicació del DNA fins al començament de l’anafase. Aquesta funció, coneguda com a Cohesió entre Cromàtides Germanes, permet la biorientació dels cromosomes en el fus mitòtic i, posteriorment, la seva correcta segregació. Es tracta per tant d’una funció fonamental per a la vida. La cohesió entre cromàtides germanes també té altres funcions, com ara afavorir la reparació del dany en el DNA a través de recombinació homòloga. É
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Page, Andrea Wilder. "The meiotic cell cycle and sister-chromatid cohesion in Drosophila oocytes." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/9843.

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Borges, V. S. F. "Establishment of sister chromatid cohesion during DNA replication in Saccharomyces cerevisiae." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1370644/.

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Анотація:
Establishment of sister chromatid cohesion is a process thought to occur as the replication fork passes chromosomal loci bound by the cohesin complex. After fork passage, cohesin holds together pairs of replication products to allow their recognition by the mitotic machinery for segregation into daughter cells. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of sister chromatid cohesion. At the time of anaphase, Smc3
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Sundaramoorthy, S. "Mediators of pre-mRNA splicing regulate sister chromatid cohesion in mammalian cells." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1418244/.

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Анотація:
The ‘endless forms most beautiful’ that populate our planet rely on the process of cell division to ensure equal segregation of the cellular content including the DNA to the two daughter cells. The accurate segregation of chromosomes in eukaryotes relies on connection between replicated sister chromatids, a phenomenon known as sister chromatid cohesion. Sister chromatid cohesion is mediated by a conserved ring-like protein complex known as cohesin. Defects in this process can promote aneuploidy and contribute to meiotic segregation errors with adverse consequences for developing embryos. Despi
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Vickridge, Elise. "Management of E. coli sister chromatid cohesion in response to genotoxic stress." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS172/document.

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La réplication fidèle de l’ADN au cours du cycle cellulaire est essentielle au maintien de l’intégrité du génome à travers les générations. Toutefois, de nombreux éléments peuvent perturber et compromettre la réplication et donc cette intégrité. La mitomycine C (MMC) est une molécule génotoxique utilisée en chimiothérapie. Elle forme des liaisons covalentes entre les deux brins d’ADN, ce qui est un obstacle à la bonne réplication de l’ADN. La rencontre de la fourche de réplication avec une liaison covalente entre les deux brins d’ADN va aboutir à une cassure double brin. Escherichia coli (E.co
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Vickridge, Elise. "Management of E. coli sister chromatid cohesion in response to genotoxic stress." Electronic Thesis or Diss., Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS172.

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Анотація:
La réplication fidèle de l’ADN au cours du cycle cellulaire est essentielle au maintien de l’intégrité du génome à travers les générations. Toutefois, de nombreux éléments peuvent perturber et compromettre la réplication et donc cette intégrité. La mitomycine C (MMC) est une molécule génotoxique utilisée en chimiothérapie. Elle forme des liaisons covalentes entre les deux brins d’ADN, ce qui est un obstacle à la bonne réplication de l’ADN. La rencontre de la fourche de réplication avec une liaison covalente entre les deux brins d’ADN va aboutir à une cassure double brin. Escherichia coli (E.co
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Lee, Janice Ying 1974. "Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/32256.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004.<br>Includes bibliographical references.<br>In cell division, the proper segregation of chromosomes requires sister-chromatid cohesion. This physical attachment between sister chromatids is established during DNA replication, maintained throughout mitosis and released at the metaphase-anaphase transition. In meiosis, sister-chromatid cohesion is released along the chromosome arms in the first meiotic division, but retained at the centromere until the second meiotic division. In this thesis, we have analyzed the loca
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BOATENG, KINGSLEY A. "STUDIES ON ARABIDOPSIS PROTEINS REQUIRED FOR THE ESTABLISHMENT AND RELEASE OF SISTER CHROMATID COHESION." Miami University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=miami1185209243.

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Tang, Tracy Tzu-Ling 1971. "The Drosophila centrometic protein MEI-S332 : its role and regulation in sister-chromatid cohesion." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/85285.

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Книги з теми "Chromatid cohesion"

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Lam, Wendy W. S. Keeping sisters together: Cohesin and Ycs4 define distinct mechanisms for sister chromatid cohesion. 2005.

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Lam, Wendy W. S. Keeping sisters together: Cohesin and Ycs4 define distinct mechanisms for sister chromatid cohesion. 2005.

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Частини книг з теми "Chromatid cohesion"

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Orr-Weaver, T. L., D. P. Moore, A. W. Kerrebrock, S. E. Bickel, and D. W. Wyman. "Proteins controlling sister-chromatid cohesion." In Chromosomes Today. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-1537-4_16.

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Suja, J. A., and J. L. Barbero. "Cohesin Complexes and Sister Chromatid Cohesion in Mammalian Meiosis." In Meiosis. KARGER, 2008. http://dx.doi.org/10.1159/000166622.

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Feeney, Katherine M., Laura McFarlane-Majeed, and Joanna L. Parish. "Analyzing Sister Chromatid Cohesion in Mammalian Cells." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0888-2_32.

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Bickel, Sharon E., and Terry L. Orr-Weaver. "Regulation of Sister-Chromatid Cohesion During Drosophila Meiosis." In Germ Cell Development, Division, Disruption and Death. Springer New York, 1998. http://dx.doi.org/10.1007/978-1-4612-2206-4_5.

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Leman, Adam R., and Eishi Noguchi. "Linking Chromosome Duplication and Segregation via Sister Chromatid Cohesion." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0888-2_5.

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Shintomi, Keishi, and Tatsuya Hirano. "A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6545-8_1.

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Severson, Aaron F. "Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6545-8_5.

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Dorsett, Dale, and Ziva Misulovin. "Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6545-8_8.

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Prasad, Priya. "Loss of Function of Sth1, The Catalytic Component of RSC (Remodel the Structure of Chromatin) Complex Grossly Alter the Chromatin Architecture." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_17.

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AbstractChromatin architecture has a profound effect on the gene expression in eukaryotes. It is constantly modulated in the cells in response to different stress condition and during the normal physiological process in the cell. The chromatin is also modulated during the cell growth and division, where several proteins involved during the cell cycle are synthesized, and hence the gene expression profile and chromatin state of an actively dividing cell differ from that of a resting cell in G0 state. Candida albicans, which is a harmless commensal in human host and an opportunistic fungal patho
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Gupta, Preksha, Thais Lavagnolli, Hegias Mira-Bontenbal, and Matthias Merkenschlager. "Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6545-8_12.

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Тези доповідей конференцій з теми "Chromatid cohesion"

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Matsumoto, Shinya, Kan Okubo, Takuya Abe, and Kiyoshi Nishikawa. "Detection model of sister chromatid cohesion defects based on Vision Transformer." In 2023 Asia Pacific Signal and Information Processing Association Annual Summit and Conference (APSIPA ASC). IEEE, 2023. http://dx.doi.org/10.1109/apsipaasc58517.2023.10317257.

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Koedoot, Esmee, John Martens, Sylvia E. Le Dévédec, and Bob van de Water. "Abstract 1527: Splicing factors determine breast cancer cell mitosis through control of sister chromatid cohesion." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-1527.

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Viushkov, V. S., N. A. Lomov, and M. A. Rubtsov. "INVESTIGATION OF THE IMPACT OF COHESIN ON CONSTRAINING CHROMATIN DYNAMICS UNDER NORMAL CONDITIONS AND AFTER THE INDUCTION OF DOUBLE-STRAND DNA BREAKS." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-225.

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Using CRISPR-Sirius imaging in human cells with inducible degradation of the cohesin subunit RAD21, we studied the spatial dynamics of the chromatin locus upon induction of DNA double-strand breaks by etoposide, upon RAD21 degradation, and upon simultaneous RAD21 degradation and break induction. We showed that cohesin acts as a constraining factor of chromatin diffusion mobility both under normal conditions and upon the induction of double-strand breaks.
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Sosnovskaia, Sofiia. "Microscopic approach to study the role of cohesin in chromatin folding." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.1140.

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Звіти організацій з теми "Chromatid cohesion"

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Pati, Debananda. Linking Sister Chromatid Cohesion to Apoptosis and Aneuploidy in the Development of Breast Cancer. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada418197.

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