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Статті в журналах з теми "CPV-2a"

Автор: Grafiati

Опубліковано: 7 червня 2025

Оновлено: 2 серпня 2025

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1

Vimalan, Yuvachandran Viva, Raja Paramasivam, Parthiban Manoharan, et al. "Genomic Sequence and Phylogenetic analysis of Canine Parvovirus New 2a Variant Strain in Indian Dogs." Indian Journal of Veterinary Sciences & Biotechnology 19, no. 2 (2023): 47–53. http://dx.doi.org/10.48165/ijvsbt.19.2.09.

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Анотація:

In this study, a virulent field canine parvovirus type - 2a variant (CPV-new 2a) strain, ABT/CPV/MVC02, was identified from the faeces of a Dog with severe haemorrhagic gastroenteritis in Chennai, Tamil Nadu, India. The genomic sequence of ABT/CPV/MVC02 (MN661243) shares 99.33% nucleotide identity with those of CPV new 2a variant strains. Based on the VP2 capsid protein amino acid variation (Ser297Ala), the ABT/CPV/MVC02 strain is classified as a new CPV 2a variant strain. Two VP2 mutations Tyr324Ile and Thr440Ala were reported in most of the CPV-2a stains also present in ABT/CPV/MVC02.The phy

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2

Giraldo-Ramirez, Sebastián, Santiago Rendon-Marin, and Julián Ruiz-Saenz. "Phylogenetic, Evolutionary and Structural Analysis of Canine Parvovirus (CPV-2) Antigenic Variants Circulating in Colombia." Viruses 12, no. 5 (2020): 500. http://dx.doi.org/10.3390/v12050500.

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Анотація:

Canine parvovirus (CPV-2) is the causative agent of haemorrhagic gastroenteritis in canids. Three antigenic variants—CPV-2a, CPV-2b and CPV-2c—have been described, which are determined by variations at residue 426 of the VP2 capsid protein. In Colombia, the CPV-2a and CPV-2b antigenic variants have previously been reported through partial VP2 sequencing. Mutations at residues Asn428Asp and Ala514Ser of variant CPV-2a were detected, implying the appearance of a possible new CPV-2a variant in Colombia. The purpose of the present study was to characterise the full VP2 capsid protein in samples fr

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3

Inthong, N., S. Kaewmongkol, N. Meekhanon, K. Sirinarumitr, and T. Sirinarumitr. "Dynamic evolution of canine parvovirus in Thailand." February-2020 13, no. 2 (2020): 245–55. http://dx.doi.org/10.14202/vetworld.2020.245-255.

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Background and Aim: According to the previous study, the circulating canine parvovirus (CPV) in Thailand is 2a and 2b. Nowadays, CPV mutants, including CPV-2c, have been identified in many parts of the world. This study aimed to investigate the genetic diversity of the circulating CPV in Thailand. Materials and Methods: Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were an

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4

Cavalli, Alessandra, Vito Martella, Costantina Desario, et al. "Evaluation of the Antigenic Relationships among Canine Parvovirus Type 2 Variants." Clinical and Vaccine Immunology 15, no. 3 (2007): 534–39. http://dx.doi.org/10.1128/cvi.00444-07.

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ABSTRACT The antigenic relationships among the original canine parvovirus type 2 (CPV-2) and the variants CPV-2a, -2b, and -2c were evaluated. Cross-antigenic evaluation revealed clear differences among the CPV variants, which were more appreciable by serum neutralization (SN) than by hemagglutination inhibition. Antigenic differences were found mostly between the original CPV-2 and the variants, but they were also observed among the variants CPV-2a, -2b, and -2c. The variant CPV-2c exhibited a unique antigenic pattern, since it was poorly recognized by the sera of animals immunized with CPV-2

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5

Pavana Jyoth, Vanjavaka, Mohana Subramanian Bhaskaran, and Vijay A. K. B. Gundi. "Epidemiology, molecular prevalence and prevention on canine parvovirus in India: A review." Bioinformation 20, no. 5 (2024): 536–46. http://dx.doi.org/10.6026/973206300200536.

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Анотація:

Canine parvovirus (CPV) is a highly contagious and lethal virus that causes severe gastroenteritis and myocarditis in young dogs. In 1978, CPV has rapidly spread worldwide, resulting in outbreaks and high morbidity rates among dog populations. Over a decade, CPV has undergone genetic changes, leading to the emergence of different genotypes (CPV-2a, CPV-2b, and CPV-2c), which have expanded its host range to include cats and tissue culture cells. This review focuses on CPV-2 outbreaks in India from 2010 to 2023, analyzing gene lengths covering 274-438 amino acids in the VP2 gene which are collec

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6

Maganga, Gael Darren, Ingrid Labouba, Serda Zita Milendz Ikapi, et al. "Molecular Characterization of Canine Parvovirus Variants CPV-2a and CPV-2c, Associated with Vaccinated Dogs at Libreville, Gabon." Viruses 15, no. 5 (2023): 1169. http://dx.doi.org/10.3390/v15051169.

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Анотація:

The first detection of canine parvovirus type-2 (CPV-2) was in the early 1970s, when it was known to cause severe gastroenteritis in dogs. However, it has evolved over the years into CPV-2a within 2 years, into CPV-2b after 14 years, into CPV-2c after 16 years and more recently CPV-2a-, 2b- and 2c-like variants reported in 2019, with a global distribution. Reports on the molecular epidemiology of this virus are missing in most African countries. The report of clinical cases among vaccinated dogs in Libreville in Gabon triggered the execution of this study. The objective of this study was to ch

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7

Pereira, Nicole Assis, Telma Alves Monez, César Augusto Dinóla Pereira, Leonardo José Richtzenhain, and Edison Luiz Durigon. "Caracterização antigênica de isolados de parvovirus canino do Brasil utilizando monoclonais específicos." Brazilian Journal of Veterinary Research and Animal Science 45, no. 5 (2008): 385. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2008.26680.

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O Parvovírus Canino (CPV) é um patógeno emergente em cães, isolado pela primeira vez em 1978, nos Estados Unidos. A amostra original de 1978 foi designada CPV tipo 2 (CPV-2). Entretanto, análises de isolados de CPV dos Estados Unidos, por enzimas de restrição e anticorpos monoclonais demonstraram que cerca de 1979, uma amostra variante, designada CPV tipo 2a (CPV-2a) tornou-se prevalente. Subseqüentemente, uma nova amostra antigênica, designada CPV tipo 2b (CPV-2b) também foi observada por análises de isolados de CPV de várias partes do mundo, embora a proporção fosse diferente entre os países

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8

Gainor, Kerry, April Bowen, Pompei Bolfa, Andrea Peda, Yashpal S. Malik, and Souvik Ghosh. "Molecular Investigation of Canine Parvovirus-2 (CPV-2) Outbreak in Nevis Island: Analysis of the Nearly Complete Genomes of CPV-2 Strains from the Caribbean Region." Viruses 13, no. 6 (2021): 1083. http://dx.doi.org/10.3390/v13061083.

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Анотація:

To date, there is a dearth of information on canine parvovirus-2 (CPV-2) from the Caribbean region. During August–October 2020, the veterinary clinic on the Caribbean island of Nevis reported 64 household dogs with CPV-2-like clinical signs (hemorrhagic/non-hemorrhagic diarrhea and vomiting), of which 27 animals died. Rectal swabs/fecal samples were obtained from 43 dogs. A total of 39 of the 43 dogs tested positive for CPV-2 antigen and/or DNA, while 4 samples, negative for CPV-2 antigen, were not available for PCR. Among the 21 untested dogs, 15 had CPV-2 positive littermates. Analysis of th

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9

Nakamura, Masato, Kazuya Nakamura, Takayuki Miyazawa, Yukinobu Tohya, Masami Mochizuki, and Hiroomi Akashi. "Monoclonal Antibodies That Distinguish Antigenic Variants of Canine Parvovirus." Clinical Diagnostic Laboratory Immunology 10, no. 6 (2003): 1085–89. http://dx.doi.org/10.1128/cdli.10.6.1085-1089.2003.

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Анотація:

ABSTRACT Canine parvovirus (CPV) is classified as a member of the feline parvovirus (FPV) subgroup. CPV isolates are divided into three antigenic types: CPV type 2 (CPV-2), CPV-2a, and CPV-2b. Recently, new antigenic types of CPV were isolated from Vietnamese leopard cats and designated CPV-2c(a) or CPV-2c(b). CPV-2c viruses were distinguished from the other antigenic types of the FPV subgroup by the absence of reactivity with several monoclonal antibodies (MAbs). To characterize the antigenicity of CPV-2c, a panel of MAbs against CPV-2c was generated and epitopes recognized by these MAbs were

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10

Tumenjargal, Sh, Ts Ariunaa, L. Ganbayar, G. Otgontuya, B. Chimedtseren, and Ch Tungalag. "Predominance of canine parvovirus type 2b in dogs of Ulaanbaatar City." Mongolian Journal of Agricultural Sciences 15, no. 2 (2015): 71–74. http://dx.doi.org/10.5564/mjas.v15i2.549.

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Анотація:

Canine parvovirus is a highly contagious virus that causes fatal disease acute hemorrhagic enteritis and myocarditis in dogs. The aim of this work is to detect canine parvovirus 2 (CPV-2) by standard polymerase chain reaction (PCR). Viral DNA was isolated from faecel samples of 36 puppies with suspicious symptoms for parvovirus infection and used as template in standard PCR. 23 samples wereof CPV-2b serotype, 9 samples of CPV-2a serotype but 4 samples were neither 2b and nor 2a. We used two different primer sets, one specific both serotypes CPV-2a and CPV-2b and one specific only for CPV-2b. T

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11

Organtini, Lindsey J., Andrew B. Allison, Tiit Lukk, Colin R. Parrish, and Susan Hafenstein. "Global Displacement of Canine Parvovirus by a Host-Adapted Variant: Structural Comparison between Pandemic Viruses with Distinct Host Ranges." Journal of Virology 89, no. 3 (2014): 1909–12. http://dx.doi.org/10.1128/jvi.02611-14.

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Анотація:

Canine parvovirus type 2 (CPV-2) emerged in 1978 and spread worldwide within 2 years. Subsequently, CPV-2 was completely replaced by the variant CPV-2a, which is characterized by four specific capsid (VP2) mutations. The X-ray crystal structure of the CPV-2a capsid shows that each mutation confers small local changes. The loss of a hydrogen bond and introduction of a glycine residue likely introduce flexibility to sites that control interactions with the host receptor, antibodies, and sialic acids.

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12

Sung, Ming-Hua, Tsung-Ming Chen, Yi-Chen Yang, et al. "MOLECULAR EPIDEMIOLOGY OFCANINE PARVOVIRUS TYPE2A AND 2B AMONG SHELTER DOGS — THE NEW RECOMBINATION IN VIRUS." Taiwan Veterinary Journal 43, no. 01 (2017): 47–53. http://dx.doi.org/10.1142/s1682648515500341.

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Анотація:

Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen mainly affecting dogs. CPV-2 contains three antigenic variants (2a, 2b, and 2c) that are distributed internationally. Detection and characterization of the currently circulating CPV-2 strains are vital for the understanding of viral evolution, transmission, and the development of methods to control its spreading. Herein, we analyzed the strains in central Taiwan to provide information of local viral evolution, diversity, and epidemiology. Stool and blood samples from 24 dogs and 2 cats were genotyped by PCR amplification of strain-s

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13

KOUR, KARMAN, GURPREET KAUR, MUDIT CHANDRA, and P. N. DWIVEDI. "Real time PCR typing of canine parvovirus types in various regions of northern India." Indian Journal of Animal Sciences 92, no. 8 (2022): 931–34. http://dx.doi.org/10.56093/ijans.v92i8.106851.

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Анотація:

Canine parvovirus (CPV) is a ssDNA virus which has high morbidity in unvaccinated and some vaccinated dogs as well. It is seen to be evolving and has various antigenic types. The present study was aimed to study the prevalence of the antigenic type in northern India by real-time PCR. This study was conducted in 2019 wherein 178 samples (collected from Delhi, Punjab, Jammu, Chandigarh and Assam) available in the Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana were subjected to real time PCR for CPV 2, CPV 2

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14

Rakib, Tofazzal Md, Babu Kanti Nath, Tridip Das, Saroj Kumar Yadav, Shane R. Raidal, and Shubhagata Das. "Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh." Pathogens 10, no. 11 (2021): 1373. http://dx.doi.org/10.3390/pathogens10111373.

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Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspect

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15

Monteiro, Kyssia, Susan D. Allendorf, Acácia F. Vicente, et al. "Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil." Pesquisa Veterinária Brasileira 36, no. 12 (2016): 1181–85. http://dx.doi.org/10.1590/s0100-736x2016001200007.

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ABSTRACT: Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70’s new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central-West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All

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16

Hao, Xiangqi, Yanchao Li, Xiangyu Xiao, Bo Chen, Pei Zhou, and Shoujun Li. "The Changes in Canine Parvovirus Variants over the Years." International Journal of Molecular Sciences 23, no. 19 (2022): 11540. http://dx.doi.org/10.3390/ijms231911540.

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Анотація:

Canine parvovirus (CPV-2) is one of the most important pathogens in dogs, and despite the continual development of vaccines against CPV-2, CPV-2 is still circulating in the canine population. The CPV-2a/2b/2c variant has replaced the original CPV-2 virus and seems to exhibit accelerated transmission. Although CPV-2 infection has been frequently reported, no studies have summarized information of CPV-2 variants currently circulating worldwide. To track the evolution of CPV-2, we downloaded and analyzed all VP2 sequences from the NCBI database (from 1978 to 2022). We found that CPV-2c shows a te

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17

Loor-Giler, Anthony, Silvana Santander-Parra, Sara Castillo-Reyes, et al. "Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022–2023." Veterinary Sciences 12, no. 1 (2025): 46. https://doi.org/10.3390/vetsci12010046.

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Анотація:

Canine parvovirus (CPV-2) is a highly contagious virus in canines, and it is mostly spread by touching infected feces. Dogs of all ages can catch it, but puppies are more likely to suffer from it. Severe signs include vomiting, diarrhea with blood, feeling tired, and not drinking enough water. There are three different types of the original CPV-2 that have been found so far, which are CPV-2a, 2b, and 2c. The genome of CPV-2 is about 5.2 kb long and has two open reading frames (ORFs), namely the VP region and the NS region. Based on changes in amino acids at position 426, the VP2 protein distin

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18

Jantafong, Tippawan, Sakchai Ruenphet, Harold R. Garner, and Krit Ritthipichai. "Tracing the Genetic Evolution of Canine Parvovirus Type 2 (CPV-2) in Thailand." Pathogens 11, no. 12 (2022): 1460. http://dx.doi.org/10.3390/pathogens11121460.

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Canine parvovirus type 2 (CPV-2) is responsible for hemorrhagic gastroenteritis in dogs worldwide. High genomic substitution rates in CPV-2 contribute to the progressive emergence of novel variants with increased ability to evade the host immune response. Three studies have analyzed the genomic mutations of CPV-2 variants in Thailand. These investigations were independently conducted at different timepoints. Thus, a retrospective integrated analysis of CPV-2 genomic mutations has not been fully performed. Our study aimed at evaluating the evolutionary changes in CPV-2 in Thailand from 2003 to

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19

Mandawat, Saksham, Rashmi Singh, Nazeer Mohammed, et al. "Comparative Pathophysiology of Canine Parvovirus-2 Variants in Naturally Infected Dogs." Asian Journal of Research in Biochemistry 15, no. 3 (2025): 137–44. https://doi.org/10.9734/ajrb/2025/v15i3395.

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Canine Parvovirus-2 (CPV-2) is a highly lethal virus that affects the canid family. It targets rapidly dividing cells in organs such as the intestines, thymus, lymph nodes, bone marrow, and heart, often resulting in multi-organ failure.This study aimed to evaluate the impact of CPV-2 variants on clinico-physiological, haematological and biochemical parameters in dogs. Two hundred faecal and blood samples from symptomatic dogs, along with 10 healthy controls, were collected at VCC, PGIVER, Jaipur, from July 2024 to December 2024. PCR identified 166 CPV-2 positive cases, comprising 147 CPV-2a, 1

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20

Giuseppa, Purpari, Mira Francesco, Di Bella Santina, Di Pietro Simona, Giudice Elisabetta, and Guercio Annalisa. "Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay." Acta Veterinaria 68, no. 1 (2018): 80–94. http://dx.doi.org/10.2478/acve-2018-0007.

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Abstract Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and thes

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21

WÓJCIK, ALICJA, JERZY ZIĘTEK, MARTA STANIEC, and STANISŁAW WINIARCZYK. "Genetic variability among canine parvovirus strains currently circulating in Poland." Medycyna Weterynaryjna 77, no. 05 (2021): 6525–2021. http://dx.doi.org/10.21521/mw.6525.

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Анотація:

The aim of the study was to perform a molecular analysis of the CPV VP2 gene fragment isolated from faeces samples of 46 dogs (vaccinated, as well as non-vaccinated against parvovirosis) with clinical parvovirosis. In all animals, the infection was confirmed by PCR. The purified PCR products (fragment VP2 gene) were sequenced and compared to each other and to the sequences of CPV VP2 gene from NCBI GenBank. Readable sequences of the VP2 gene were obtained for 46 samples. In 23 out of 30 non-vaccinated dogs, the most frequently isolated CPV strain was 2b. In the remaining cases the dogs were in

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22

Nakamura, Kazuya, Maki Sakamoto, Yasuhiro Ikeda, et al. "Pathogenic Potential of Canine Parvovirus Types 2a and 2c in Domestic Cats." Clinical Diagnostic Laboratory Immunology 8, no. 3 (2001): 663–68. http://dx.doi.org/10.1128/cdli.8.3.663-668.2001.

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ABSTRACT The in vivo pathogenicity of canine parvovirus (CPV) type 2c (strain V203) and of CPV type 2a (strain V154) against cats was investigated. Our results indicate that both types of CPV have the potential to induce disease in cats.

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23

Zienius, D., R. Lelešius, H. Kavaliauskis, A. Stankevičius, and A. Šalomskas. "Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples." Polish Journal of Veterinary Sciences 19, no. 1 (2016): 187–96. http://dx.doi.org/10.1515/pjvs-2016-0023.

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Abstract The aim of the present study was to detect canine parvovirus (CPV) from faecal samples of clinically ill domestic dogs by polymerase chain reaction (PCR) followed by VP2 gene partial sequencing and molecular characterization of circulating strains in Lithuania. Eleven clinically and antigen-tested positive dog faecal samples, collected during the period of 2014-2015, were investigated by using PCR. The phylogenetic investigations indicated that the Lithuanian CPV VP2 partial sequences (3025-3706 cds) were closely related and showed 99.0-99.9% identity. All Lithuanian sequences were as

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24

Hong, Charles, Nicola Decaro, Costantina Desario, et al. "Occurrence of Canine Parvovirus Type 2c in the United States." Journal of Veterinary Diagnostic Investigation 19, no. 5 (2007): 535–39. http://dx.doi.org/10.1177/104063870701900512.

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Анотація:

Canine parvovirus (CPV) type 2 (CPV-2) emerged around 1978 as a major pathogen of dogs worldwide. In the mid-1980s, the original CPV-2 had evolved and was completely replaced by 2 variants, CPV-2a and CPV-2b. In 2000, a new variant of CPV (named CPV-2c) was detected in Italy and now cocirculates with types 2a and 2b in that country. The CPV-2c has also been reported from single outbreaks in Vietnam and Spain. This study was conducted to determine if CPV-2c occurs in the United States. Thirty-three fecal samples were collected from dogs in 16 states between April 2006 and April 2007 and were te

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25

Jinadasa, Rasika, Sayani Ghosh, Simon Hills, et al. "Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka." Pathogens 10, no. 9 (2021): 1102. http://dx.doi.org/10.3390/pathogens10091102.

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Анотація:

The aim of the study was to identify canine parvovirus type 2 (CPV-2) subtypes circulating among a selected population of domestic dogs and cats in Sri Lanka and to investigate the evolutionary patterns among Sri Lankan viruses in the context of contemporary global CPV-2 sequences. Altogether, 40/61 (65.6%) samples tested were positive for CPV-2 DNA, including 31/48 (64.6%) dogs and 9/13 (69%) cats. All three subtypes (CPV-2a, CPV-2b and CPV-2c) were detected, with CPV-2a being most common. International median joining haplotype network of 291 CPV-2 sequences suggested that there was little ba

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26

Mukhopadhyay, Hirak K., Mangadevi Nookala, Nobal RK Thangamani, et al. "Molecular characterisation of parvoviruses from domestic cats reveals emergence of newer variants in India." Journal of Feline Medicine and Surgery 19, no. 8 (2016): 846–52. http://dx.doi.org/10.1177/1098612x16661375.

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Objectives The present study was undertaken to characterise the viral polypeptide 2 (VP2) gene of parvovirus from domestic cats in India. Methods The faecal samples from diarrhoeic/healthy domestic cats were collected from different geographical regions of India for screening by PCR assay followed by sequence analysis of the VP2 gene. Results Canine parvovirus (CPV)/feline panleukopenia virus (FPV) infections were found in 12 (11.3%) of 106 faecal samples tested. Two new CPV-2a (297Ala and Asn426) and three FPV strains were identified by VP2 gene analysis. Several unique and existing amino aci

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27

Kowalczyk, Marek, Barbara Majer-Dziedzic, Krzysztof Kostro, et al. "Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland." Acta Veterinaria 69, no. 1 (2019): 32–46. http://dx.doi.org/10.2478/acve-2019-0002.

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Abstract Canine parvovirus type 2 is one of the most common causes of death among puppies. Despite preventive vaccination, the disease continues to be diagnosed. The aim of the study was to provide a molecular characterization of CPV-2 isolates found in southeastern Poland. Genetic CPV-2 material was isolated from the blood (n=10) and feces (n=50) of infected dogs. The presence of CPV-2 was confirmed by amplification of sequences coding both VP1 and VP2 protein. The products of the PCR reaction with primers amplifying VP2 protein were sequenced and used for genotyping. Bioinformatics analysis

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Magouz, AF, I. Elkon, E. Khaled, and N. Alkhalefa. "First report of detection of Canine Parvovirus type 2 in naturally infected domestic cats in Egypt by duplex PCR for simultaneous detection of Canine Parvovirus type 2 and Feline Panleukopenia virus." Journal of the Hellenic Veterinary Medical Society 74, no. 3 (2023): 5921–28. http://dx.doi.org/10.12681/jhvms.28838.

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Feline Panleukopenia virus (FPLV) and Canine Parvovirus type 2 (CPV) cause fatal gastroenteritis in cats and dogs. In this study we developed a duplex polymerase chain reaction (dPCR) assay for the concurrent detection of FPLV and CPV-2 in a single PCR tube. Two primers were used based on nucleic acid conserved regions of the two viruses which specifically amplify 237 bp of the VP2 gene of FPLV and 583 bp of the VP2 gene of CPV 2.Sensitivity and Specificity of the dPCR were evaluated. A total of 30 rectal/fecal swabs were collected from domestic cats in Kafrelsheikh province, Egypt and were te

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Singh, Parikshit, Gurpreet Kaur, Mudit Chandra, and P. N. Dwivedi. "Prevalence and molecular characterization of canine parvovirus." March-2021 14, no. 3 (2021): 603–6. http://dx.doi.org/10.14202/vetworld.2021.603-606.

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Background and Aim: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. Materials and Methods: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of

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Pérez, Ruben, Pablo Bianchi, Lucía Calleros, et al. "Recent spreading of a divergent canine parvovirus type 2a (CPV-2a) strain in a CPV-2c homogenous population." Veterinary Microbiology 155, no. 2-4 (2012): 214–19. http://dx.doi.org/10.1016/j.vetmic.2011.09.017.

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Steinel, A., L. Munson, M. van Vuuren, and U. Truyen. "Genetic characterization of feline parvovirus sequences from various carnivores." Microbiology 81, no. 2 (2000): 345–50. http://dx.doi.org/10.1099/0022-1317-81-2-345.

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Infections with viruses of the feline parvovirus subgroup such as feline panleukopenia virus (FPV), mink enteritis virus (MEV) and canine parvovirus (CPV-2) [together with its new antigenic types (CPV-2a, CPV-2b)] have been reported from several wild carnivore species. To examine the susceptibility of different species to the various parvoviruses and their antigenic types, samples from wild carnivores with acute parvovirus infections were collected. Viral DNA was amplified, and subsequently analysed, from faeces or formalin-fixed small intestines from an orphaned bat-eared fox (Otocyon megalot

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SINGH, DEEPTI, AMIT KUMAR VERMA, AMIT KUMAR, and MUKESH KUMAR SRIVASTAVA. "Molecular typing of canine parvovirus with PCR assay based on genetic markers of the antigenic variants." Indian Journal of Animal Sciences 85, no. 1 (2015): 12–15. http://dx.doi.org/10.56093/ijans.v85i1.46118.

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The canine parvovirus (CPV) is prevalent in India but only scanty literature is available on molecular differentiation of its strain using polymerase chain reaction. In the present study, 100 faecal samples from dogs suspected for canine parvo virus infection (gastroenteritis) were collected and processed for isolation of viral genomic DNA. Molecular test like PCR assay using 3 specific pairs of primers (pCPV-RT, pCPV-2ab and pCPV- 2b) was conducted for differential diagnosis of the virus type. Out of 100 faecal samples, 63 were positive for presence of CPV, of which 54 were CPV-2b and 9 were

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Fontana, Danúbia S., Paulo Ricardo D. Rocha, Raquel A. S. Cruz, et al. "A phylogenetic study of canine parvovirus type 2c in midwestern Brazil." Pesquisa Veterinária Brasileira 33, no. 2 (2013): 214–18. http://dx.doi.org/10.1590/s0100-736x2013000200013.

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Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-

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Hyeon, Lee-Sang, Dong-Kun Yang, Eun-Ju Kim, Yu-Ri Park, Hye Jeong Lee, and Bang-Hun Hyun. "Evaluation of commercial immunochromatography test kits for diagnosing canine parvovirus." Korean Journal of Veterinary Research 63, no. 2 (2023): e19. http://dx.doi.org/10.14405/kjvr.20230016.

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Rapid immunochromatography test (RICT) kits are commonly used for the diagnosis of canine parvovirus (CPV) because of their rapid turnaround time, simplicity, and ease of use. However, the potential for cross-reactivity and low sensitivity can yield false-positive or false-negative results. There are 4 genotypes of CPV. Therefore, evaluating the performance and reliability of RICT kits for CPV detection is essential to ensure accurate diagnosis for appropriate treatment. In this study, we evaluated the performance of commercial RICT kits in the diagnosis of all CPV genotypes. The cross-reactiv

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Uronu, C. E., K. Said, A. P. Muhairwa, and A. A. Chengula. "Molecular detection and characterization of canine parvovirus variants in Morogoro and Arusha regions of Tanzania." Tanzania Veterinary Journal 39, no. 1 (2024): 1–12. https://doi.org/10.4314/tvj.v39i1.1.

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Canine parvovirus (CPV) is a significant pathogen that affects wild and domestic dogs globally, resulting in intricate pathological and epidemiological consequences, primarily observed in puppies, such as haemorrhagic enteritis and myocarditis. This virus is categorized into three strains: CPV-2a (which replaced the original strain CPV-2), CPV-2b, and CPV-2c. The objective of this study was to determine the status of CPV-2 and its circulating variants in domestic dogs residing in the Morogoro and Arusha regions of Tanzania. Rectal swabs were collected from a total of 143 domestic dogs with gas

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Jeoung, Seok-Young. "Evaluation for Protective Effect of CPV-2 and CPV-2b Vaccines against a Korean CPV-2a Isolate in Pups." Journal of Veterinary Clinics 31, no. 5 (2014): 361. http://dx.doi.org/10.17555/ksvc.2014.10.31.5.361.

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Yen, Sheng-Lun, Minh Hoang, Wei-Chen Wang, et al. "DETECTION OF CANINE PARVOVIRUS TYPE 2 BY A COMMERCIALLY AVAILABLE IN-HOUSE PCR." Taiwan Veterinary Journal 46, no. 01 (2020): 37–43. http://dx.doi.org/10.1142/s1682648519500069.

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Canine parvovirus type 2 (CPV-2) is an important pathogen that causes anorexia, vomiting, acute gastroenteritis, and bloody diarrhea. CPV-2 was divided into three variants (2a–2c) based on residue 426 of the VP2 protein. Recently, a novel Asian CPV-2c isolate was more prevalent in the Asian continent. The diagnostic tools of CPV-2 are characterized into traditional (such as immunochromatography test) and molecular methods based on their pathogen detection mechanisms. However, the low sensitivity of the immunochromatographic tests is supported by their simplicity and rapid ability to provide re

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Wilson, Stephen, Joanna Illambas, Elisabeth Siedek, et al. "Vaccination of dogs with canine parvovirus type 2b (CPV-2b) induces neutralising antibody responses to CPV-2a and CPV-2c." Vaccine 32, no. 42 (2014): 5420–24. http://dx.doi.org/10.1016/j.vaccine.2014.07.102.

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Pan, Shunshun, Yuanzhuo Man, Xin Xu, et al. "Genetic Diversity and Recombination Analysis of Canine Parvoviruses Prevalent in Central and Eastern China, from 2020 to 2023." Microorganisms 12, no. 11 (2024): 2173. http://dx.doi.org/10.3390/microorganisms12112173.

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Canine parvovirus type-2 (CPV-2), the primary causative agent of serious canine enteric diseases, is highly contagious and associated with high fatality rates worldwide. To comprehend the current emergence of CPV-2 in central and eastern China, 130 rectal swabs from domestic or stray dogs with gastroenteritis symptoms were collected during 2020–2023. A total of 118 positive samples were detected via polymerase chain reaction, and further used to amplify and sequence the VP2 gene. Sequence analysis of the deduced amino acids of VP2 protein indicated that CPV-2c was the most prevalent variant (n

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Alexis, Véliz-Ahumada, Vidal Sonia, Siel Daniela, et al. "Molecular Analysis of Full-Length VP2 of Canine Parvovirus Reveals Antigenic Drift in CPV-2b and CPV-2c Variants in Central Chile." Animals 11, no. 8 (2021): 2387. http://dx.doi.org/10.3390/ani11082387.

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Canine parvovirus (CPV) is a major pathogen in canines, with a high mortality rate in unvaccinated puppies. CPV is traditionally classified into three antigenic variants (CPV-2a, CPV-2b and CPV-2c) based on the amino acid sequence of the VP2 protein. Currently, various mutations are described in the receptor-binding area or in the regions of greatest antigenicity of the VP2 protein, giving rise to new viral variants that are capable of immunological escape, affecting the protective immunity of traditional vaccines. In the present study, a molecular characterization of the VP2 gene was performe

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Kelman, Mark, Lana Harriott, Maura Carrai, Emily Kwan, Michael P. Ward, and Vanessa R. Barrs. "Phylogenetic and Geospatial Evidence of Canine Parvovirus Transmission between Wild Dogs and Domestic Dogs at the Urban Fringe in Australia." Viruses 12, no. 6 (2020): 663. http://dx.doi.org/10.3390/v12060663.

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Canine parvovirus (CPV) is an important cause of disease in domestic dogs. Sporadic cases and outbreaks occur across Australia and worldwide and are associated with high morbidity and mortality. Whether transmission of CPV occurs between owned dogs and populations of wild dogs, including Canis familiaris, Canis lupus dingo and hybrids, is not known. To investigate the role of wild dogs in CPV epidemiology in Australia, PCR was used to detect CPV DNA in tissue from wild dogs culled in the peri-urban regions of two Australian states, between August 2012 and May 2015. CPV DNA was detected in 4.7%

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Hu, Wen, Liangyan Zheng, Xin Xu, et al. "Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019." Journal of Veterinary Research 64, no. 4 (2020): 477–86. http://dx.doi.org/10.2478/jvetres-2020-0076.

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AbstractIntroductionCanine parvovirus type-2 (CPV-2) causes acute infectious diseases in puppies, which show high morbidity and mortality. Better effect of vaccination against these diseases could be achieved with deeper knowledge of CPV-2 genotype dissemination and mutation history. This study investigated CPV-2–positive samples collected recently over a wide region of China.Material and MethodsA total of 118 faecal samples from dogs identified as CPV-positive were collected from veterinary clinics in central and eastern China. Overall, 16 strains collected from Anhui, 29 from Henan, and 16 f

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Shivani, Barsain Subhash Verma Rajesh Chahota Prasenjit Dhar and Monika Bhardwaj. "Key strains of canine parvovirus 2 in india: a short communication." Vet Farm Frontier 02, no. 04 (2025): 19–22. https://doi.org/10.5281/zenodo.15362298.

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Canine Parvovirus (CPV) is a highly infectious viral illness that primarily affects dogs, especially puppies aged between six weeks and six months. It is caused by Canine Parvovirus type 2 (CPV-2), which mainly targets the gastrointestinal system and, in some instances, the heart. The virus attacks rapidly dividing cells such as those in the intestinal crypts, bone marrow, and lymphoid tissues, resulting in enteritis, leukopenia, and immunosuppression. Since its discovery, CPV-2 has evolved into multiple strains, contributing to its high virulence. This article focuses on the predominant CPV-2

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Sharma, Kishan Kumar, Irsadullakhan Habibullakhan Kalyani, Shailee Manishbhai Pandya, and Jignesh Alabhai Vala. "Diagnosis and characterization of canine parvovirus-2 affecting canines of South Gujarat, India." Acta Veterinaria Brno 87, no. 3 (2018): 247–54. http://dx.doi.org/10.2754/avb201887030247.

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The present study was carried out in the region of South Gujarat, India, to determine the prevalence and predisposing factors of canine parvovirus-2 (CPV-2) infection in acute gastroenteritis of pups. Further, haemagglutination (HA) test, enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and rapid immunochromatography test were compared for diagnosis and prevalent CPV-2 types were ascertained. A total of 73 diarrhoea samples were collected and out of those 32, 33 and 35 were found positive via HA, ELISA and PCR, respectively. In rapid test, 26/52 samples were found pos

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Ndiana, L. A., G. N. Odaibo, and D. O. Olaleye. "Molecular characterization of canine parvovirus from domestic dogs in Nigeria: Introduction and spread of a CPV-2c mutant and replacement of older CPV-2a by the “new CPV-2a” strain." VirusDisease 32, no. 2 (2021): 361–68. http://dx.doi.org/10.1007/s13337-021-00689-0.

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WÓJCIK, ALICJA, STANISŁAW WINIARCZYK, and JERZY ZIĘTEK. "Canine parvovirosis: Still an existing problem." Medycyna Weterynaryjna 75, no. 04 (2019): 6191–2019. http://dx.doi.org/10.21521/mw.6191.

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Parvoviral infections of dogs are still a clinical problem throughout the world. This is despite the development of prevention, increased awareness among the owners and increasingly effective treatment protocols due to the extremely high virulence of the virus. Parvovirosis is a disease caused by a virus of the Parvoviridae family in various variants: CPV-2, CPV-2a, CPV-2b and CPV-2c, all of which produce similar clinical signs, including acute hemorrhagic enteritis and myocarditis. It is one of the most important viral pathogens, with extremely high morbidity and mortality. This study is a co

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Yip, Hiu Ying Esther, Anne Peaston, Lucy Woolford, et al. "Diagnostic Challenges in Canine Parvovirus 2c in Vaccine Failure Cases." Viruses 12, no. 9 (2020): 980. http://dx.doi.org/10.3390/v12090980.

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In this study, three different diagnostic tests for parvovirus were compared with vaccination status and parvovirus genotype in suspected canine parvovirus cases. Faecal samples from vaccinated (N17) and unvaccinated or unknown vaccination status (N41) dogs that had clinical signs of parvovirus infection were tested using three different assays of antigen tests, conventional and quantitative PCR tests. The genotype of each sample was determined by sequencing. In addition to the suspected parvovirus samples, 21 faecal samples from apparently healthy dogs were tested in three diagnostic tests to

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Hasırcıoğlu, Sibel, and Hatice Pelin Aslım. "Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR." Turkish Journal of Agriculture - Food Science and Technology 10, no. 2 (2022): 254–59. http://dx.doi.org/10.24925/turjaf.v10i2.254-259.4653.

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Canine parvovirus (CPV) is a pathogen causing hemorrhagic enteritis in puppies and mainly transmitting via feco-oral route. In this study, stool samples were collected from a total of 35 animals suspected of CPV. The samples were examined by Conventional PCR, Nested PCR and Differential PCR tests. 20 out of 35 dogs (57.1%) were detected positive by conventional PCR, 31 (88.6%) by nested PCR and 30 (85.7%) by differential PCR. CPV 2a was stated as the most common antigenic type, male animals and 0–3-month-olds had a high rate of becoming sick and vaccinated animals might also catch the disease,

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Bergmann, Michèle, Monika Freisl, and Katrin Hartmann. "Prophylaxe der kaninen Parvovirose." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 49, no. 02 (2021): 122–25. http://dx.doi.org/10.1055/a-1402-9476.

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ZusammenfassungDie Impfung ist die wichtigste Maßnahme zum Schutz vor kaniner Parvovirose. Daher zählt die Impfung gegen das kanine Parvovirus (CPV) zu den Core-Komponenten der Hundeimpfung. Modifizierte Lebendvakzinen (MLV) haben sich als sehr wirksam und zugleich sicher erwiesen. Sie bieten einen zuverlässigen Schutz, der bereits nach wenigen Tagen eintritt und viele Jahre anhält. MLV enthalten keine Adjuvanzien; sie sind hinsichtlich ihres allergenen und toxischen Potenzials Impfstoffen mit inaktiviertem CPV in aller Regel überlegen. Aus diesen Gründen werden MLV Impfstoffen aus inaktiviert

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WÓJCIK, ALICJA, STANISŁAW WINIARCZYK, and ŁUKASZ ADASZEK. "Research on factors that influence the course of canine parvovirosis." Medycyna Weterynaryjna 77, no. 07 (2021): 6552–2021. http://dx.doi.org/10.21521/mw.6552.

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The aim of this study was to demonstrate correlations of the course of canine parvovirus infection and the rate of cytopathic effect (CPE) on CPV-infected CCC clone 81 cell lines with the age of the affected animals, their history of CPV vaccination and the strain of the virus causing the infection. Forty-five dogs with parvovirus confirmed by rapid diagnostic tests and PCR were studied. Faecal samples from diseased animals were used to infect cell lines, which were then observed for a cytopathic effect. The statistical analysis of the results obtained demonstrated a positive correlation betwe

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