Добірка наукової літератури з теми "Domaines en rubans"

Three-finger proteins (TFPs) are small proteins with characteristic three-finger β-structural fold stabilized by the system of conserved disulfide bonds. These proteins have been found in organisms from different taxonomic groups and perform various important regulatory functions or act as components of snake venoms. Recently, four TFPs (Lystars 1–4) with unknown function were identified in the coelomic fluid proteome of starfish A. rubens. Here we analyzed the genomes of A. rubens and A. planci starfishes and predicted additional five and six proteins containing three-finger domains, respectively. One of them, named Lystar5, is expressed in A. rubens coelomocytes and has sequence homology to the human brain neuromodulator Lynx2. The three-finger structure of Lystar5 close to the structure of Lynx2 was confirmed by NMR. Similar to Lynx2, Lystar5 negatively modulated α4β2 nicotinic acetylcholine receptors (nAChRs) expressed in X. laevis oocytes. Incubation with Lystar5 decreased the expression of acetylcholine esterase and α4 and α7 nAChR subunits in the hippocampal neurons. In summary, for the first time we reported modulator of the cholinergic system in starfish.

This paper addresses the problem of the syllabic consonants in the selected Slavic and Celtic languages. We shall consider this issue through the optic of Government Phonology (henceforth GP), as defined e.g. in Harris 1994, Cyran 2003 and Gussmann 2007. Within the framework of GP, the phonological structure of morphemes is constructed in terms of the licensing and governing relations between adjacent skeletal positions – the timing slots. The prosodic positions are then projected onto the syllabic constituents of nuclei (the heads of rhymes) and onsets. In such configurations, onsets are always dependent on their nuclear licensers. A specific proposal advocated in this presentation is that onset-nucleus domains are not only licensing domains but they also constitute the so-called extension domains. It will be further maintained that the phenomenon of the syllabic consonants can be analyzed in terms of segment extension occurring within such onset-nucleus extension domains. It will be demonstrated that this solution effectively accounts for the relevant linguistic facts attested to in Polish, Czech, Slovak or Serbo-Croatian. In our analysis, the distinction between the syllabic and trapped consonants will be adopted which, as will be proposed, derives from different lexical structures of either type. Apart from the available dictionary entries, we shall rely on the data provided by Scheer (2003), Dalewska-Greń (2002) and Rubach (1997). The evidence concerning the behavior of the syllabic consonants in the Slavic languages will also be compared to the Irish situation. It will be proposed that the observable differences are contingent on both structural representations and different parameter settings in the languages under discussion.

SummaryThe asteroidal sperm-activating peptides (asterosaps) from the egg jelly bind to their sperm receptor, a membrane-bound guanylate cyclase, on the tail to activate sperm in sea stars. Asterosaps are produced as single peptides and then cleaved into shorter peptides. Sperm activation is followed by the acrosome reaction, which is subfamily specific. In order to investigate the molecular details of the asterosap–receptor interaction, corresponding cDNAs have been cloned, sequenced and analysed from the Asteriinae subfamily including Asterias amurensis, A. rubens, A. forbesi and Aphelasterias japonica, as well as Distolasterias nipon from the Coscinasteriinae subfamily. Averages of 29% and 86% identity were found from the deduced amino acid sequences in asterosap and its receptor extracellular domains, respectively, across all species examined. The phylogenic tree topology for asterosap and its receptor was similar to that of the mitochondrial cytochrome c oxidase subunit I. In spite of a certain homology, the amino acid sequences exhibited speciation. Conservation was found in the asterosap residues involved in disulphide bonding and proteinase-cleaving sites. Conversely, similarities were detected between potential asterosap-binding sites and the structure of the atrial natriuretic peptide receptor. Although the sperm-activating peptide and its receptor share certain common sequences, they may serve as barriers that ensure speciation in the sea star A. amurensis and closely related species.

Terpenes are phytochemicals found in multiple plant genera, especially aromatic herbs and conifers. Terpene content quantification is costly and complex, requiring the extraction of oil content and gas chromatography analyses. Near infrared (NIR) spectroscopy could provide an alternative quantitative method, especially if calibration can be developed with the spectra of dried plant material, which are easier and faster to acquire than oil-based spectra. Here, multispecies NIR spectroscopy calibrations were developed for total terpene content (mono- and sesquiterpenes) and for specific terpenes (α-pinene, β-pinene and myrcene) with five conifers species ( Picea glauca, Picea rubens, Pinus resinosa, Pinus strobus and Thuja occidentalis). The terpene content of fresh shoot samples was quantified with gas chromatography. The NIR spectra were measured on freeze-dried samples (n = 137). Using a subset of the samples, modified partial least squares regressions of total terpene and the three individual terpenes content were generated as a functions of the NIR spectra. The standard errors of the internal cross-validations (values between 0.25 and 2.28) and the ratio of prediction to deviation ratios (RPD values between 2.20 and 2.38) indicate that all calibrations have similar accuracy. The independent validations, however, suggest that the calibrations for total terpene and α-pinene content are more accurate (respective coefficient of determination: r2 = 0.85 and 0.82). In contrast, calibrations for β-pinene and myrcene had a low accuracy (respectively: r2 = 0.62 and 0.08), potentially because of the low concentration of these terpenes in the species studied. The calibration model fits (i.e., r2) are comparable to previously published calibration using the spectra of dried shoot samples and demonstrate the potential of this method for terpenes in conifer samples. The calibration method used could be useful in several other domains (e.g. seedling breeding program, industrial), because of the wide distribution of terpenes and especially of pinenes.

Le syndrome métabolique est fréquemment retrouvé en psychiatrie avec ses conséquences connues sur les risques cardio- et cérébrovasculaires. Le Comité de liaison alimentation nutrition (CLAN) de l’EPS Maison Blanche a réalisé suite à une enquête sur la traçabilité des indicateurs métaboliques un programme d’actions autour du dépistage de ce syndrome : équipement en matériel (pèse-personne, toise, mètre ruban), rédaction d’un protocole de dépistage prévention et prise en charge du syndrome métabolique, affiches et brochures d’information à l’attention des professionnels et des usagers, formations auprès des professionnels, traçabilité des indicateurs nutritionnels dans le dossier informatisé. Le CLAN s’est ensuite consacré à la sensibilisation de la prévention et de prise en charge du syndrome métabolique en s’intéressant à la promotion de l’activité physique. Une première journée sportive a eu lieu en 2012, réunissant 60 professionnels et usagers de tous services intra- et extrahospitaliers avec le soutien de la Fédération française de sport adapté (FFSA). L’évaluation de cette journée a confirmé l’importance de la place du sport dans la prise en soins. Une enquête dans les différentes unités a mis en évidence les activités existantes ainsi que les besoins et attentes des professionnels dans ce domaine. Une deuxième journée, plus ambitieuse, a réuni en 2013 plus d’une centaine de personnes (directeurs, administratifs, professionnels soignants, usagers) en partenariat avec la mairie de Paris, la direction de la jeunesse et des sports, et la FFSA. Son objectif, en réunissant les différents partenaires impliqués autour du moyen-sport, est de pérenniser ces actions dans le quotidien de la prise en charge avec une aide structurelle et fonctionnelle adaptée. Le projet actuel du CLAN est de créer une association à destination des usagers leur facilitant l’accès à une activité sportive.

This paper examines opaque examples of phrase-level phonology taken from Chilean Spanish under the framework of Stratal Optimality Theory (OT) (Rubach 1997; Bermúdez-Otero 2003, 2019) and Harmonic Serialism (HS) (McCarthy 2008a, b, 2016). The data show an interesting double repair of the coda /s/ taking place at word edges. It is argued that Stratal OT is superior in modelling phonological processes that take place at the interface between morphology and phonology because it embraces cyclicity. Under this model, prosodic structure is built serially, level by level, and in accordance with the morphological structure of the input string. In this way, opacity at constituent edges can be solved. Stratal OT also provides insight into word-internal morphological structure and the domain-specificity of phonological processes. It is demonstrated that a distinction in this model is necessary between the word and the phrase levels, and between the stem and the word levels. As illustrated by the behaviour of Spanish nouns, affixation and the resultant alternations inform us about the domains to which both morphological and phonological processes should be assigned. Against this background, Harmonic Serialism embraces an apparently simpler recursive mechanism in which stepwise prosodic parsing can be incorporated. What is more, it offers insight into the nature of operations in OT, as well as into such problematic issues as structure building and directionality. Nevertheless, despite the model’s ability to solve various cases of opacity, the need to distinguish between two competing repairs makes HS fail when confronted with the Chilean data under examination.

Sea stars adhere strongly but temporarily to underwater substrata via the secretion of a blend of proteins, forming an adhesive footprint that they leave on the surface after detachment. Their tube feet enclose a duo-gland adhesive system comprising two types of adhesive cells, contributing different layers of the footprint and de-adhesive cells. In this study, we characterized the catalogue of sea star footprint proteins (Sfps) in the species Asterias rubens to gain insights in their potential function. We identified 16 Sfps and mapped their expression to type 1 and/or type 2 adhesive cells or to de-adhesive cells by double fluorescent in situ hybridization. Based on their cellular expression pattern and their conserved functional domains, we propose that the identified Sfps serve different functions during attachment, with two Sfps coupling to the surface, six providing cohesive strength and the rest forming a binding matrix. Immunolabelling of footprints with antibodies directed against one protein of each category confirmed these roles. A de-adhesive gland cell-specific astacin-like proteinase presumably weakens the bond between the adhesive material and the tube foot surface during detachment. Overall, we provide a model for temporary adhesion in sea stars, including a comprehensive list of the proteins involved.

The TRP superfamily of channels (nomenclature as agreed by NC-IUPHAR [145, 915]), whose founder member is the Drosophila Trp channel, exists in mammals as six families; TRPC, TRPM, TRPV, TRPA, TRPP and TRPML based on amino acid homologies. TRP subunits contain six putative transmembrane domains and assemble as homo- or hetero-tetramers to form cation selective channels with diverse modes of activation and varied permeation properties (reviewed by [630]). Established, or potential, physiological functions of the individual members of the TRP families are discussed in detail in the recommended reviews and in a number of books [344, 589, 979, 216]. The established, or potential, involvement of TRP channels in disease is reviewed in [384, 588] and [591], together with a special edition of Biochemica et Biophysica Acta on the subject [588]. Additional disease related reviews, for pain [542], stroke [967], sensation and inflammation [843], itch [109], and airway disease [261, 896], are available. The pharmacology of most TRP channels has been advanced in recent years. Broad spectrum agents are listed in the tables along with more selective, or recently recognised, ligands that are flagged by the inclusion of a primary reference. See Rubaiy (2019) for a review of pharmacological tools for TRPC1/C4/C5 channels [692]. Most TRP channels are regulated by phosphoinostides such as PtIns(4,5)P2 although the effects reported are often complex, occasionally contradictory, and likely to be dependent upon experimental conditions, such as intracellular ATP levels (reviewed by [862, 592, 689]). Such regulation is generally not included in the tables.When thermosensitivity is mentioned, it refers specifically to a high Q10 of gating, often in the range of 10-30, but does not necessarily imply that the channel's function is to act as a 'hot' or 'cold' sensor. In general, the search for TRP activators has led to many claims for temperature sensing, mechanosensation, and lipid sensing. All proteins are of course sensitive to energies of binding, mechanical force, and temperature, but the issue is whether the proposed input is within a physiologically relevant range resulting in a response. TRPA (ankyrin) familyTRPA1 is the sole mammalian member of this group (reviewed by [246]). TRPA1 activation of sensory neurons contribute to nociception [356, 763, 516]. Pungent chemicals such as mustard oil (AITC), allicin, and cinnamaldehyde activate TRPA1 by modification of free thiol groups of cysteine side chains, especially those located in its amino terminus [491, 47, 311, 493]. Alkenals with α, β-unsaturated bonds, such as propenal (acrolein), butenal (crotylaldehyde), and 2-pentenal can react with free thiols via Michael addition and can activate TRPA1. However, potency appears to weaken as carbon chain length increases [21, 47]. Covalent modification leads to sustained activation of TRPA1. Chemicals including carvacrol, menthol, and local anesthetics reversibly activate TRPA1 by non-covalent binding [364, 438, 923, 922]. TRPA1 is not mechanosensitive under physiological conditions, but can be activated by cold temperatures [365, 175]. The electron cryo-EM structure of TRPA1 [639] indicates that it is a 6-TM homotetramer. Each subunit of the channel contains two short ‘pore helices’ pointing into the ion selectivity filter, which is big enough to allow permeation of partially hydrated Ca2+ ions. TRPC (canonical) familyMembers of the TRPC subfamily (reviewed by [239, 673, 14, 4, 79, 382, 638, 55]) fall into the subgroups outlined below. TRPC2 is a pseudogene in humans. It is generally accepted that all TRPC channels are activated downstream of Gq/11-coupled receptors, or receptor tyrosine kinases (reviewed by [661, 814, 915]). A comprehensive listing of G-protein coupled receptors that activate TRPC channels is given in [4]. Hetero-oligomeric complexes of TRPC channels and their association with proteins to form signalling complexes are detailed in [14] and [383]. TRPC channels have frequently been proposed to act as store-operated channels (SOCs) (or compenents of mulimeric complexes that form SOCs), activated by depletion of intracellular calcium stores (reviewed by [640, 14, 665, 703, 954, 132, 626, 51, 133]). However, the weight of the evidence is that they are not directly gated by conventional store-operated mechanisms, as established for Stim-gated Orai channels. TRPC channels are not mechanically gated in physiologically relevant ranges of force. All members of the TRPC family are blocked by 2-APB and SKF96365 [295, 294]. Activation of TRPC channels by lipids is discussed by [55]. Important progress has been recently made in TRPC pharmacology [692, 529, 372, 87]. TRPC channels regulate a variety of physiological functions and are implicated in many human diseases [248, 56, 759, 879]. TRPC1/C4/C5 subgroup TRPC1 alone may not form a functional ion channel [191]. TRPC4/C5 may be distinguished from other TRP channels by their potentiation by micromolar concentrations of La3+. TRPC2 is a pseudogene in humans, but in other mammals appears to be an ion channel localized to microvilli of the vomeronasal organ. It is required for normal sexual behavior in response to pheromones in mice. It may also function in the main olfactory epithelia in mice [951, 625, 624, 952, 462, 988, 947].TRPC3/C6/C7 subgroup All members are activated by diacylglycerol independent of protein kinase C stimulation [295].TRPM (melastatin) familyMembers of the TRPM subfamily (reviewed by [230, 294, 640, 978]) fall into the five subgroups outlined below. TRPM1/M3 subgroupIn darkness, glutamate released by the photoreceptors and ON-bipolar cells binds to the metabotropic glutamate receptor 6 , leading to activation of Go . This results in the closure of TRPM1. When the photoreceptors are stimulated by light, glutamate release is reduced, and TRPM1 channels are more active, resulting in cell membrane depolarization. Human TRPM1 mutations are associated with congenital stationary night blindness (CSNB), whose patients lack rod function. TRPM1 is also found melanocytes. Isoforms of TRPM1 may present in melanocytes, melanoma, brain, and retina. In melanoma cells, TRPM1 is prevalent in highly dynamic intracellular vesicular structures [341, 609]. TRPM3 (reviewed by [615]) exists as multiple splice variants which differ significantly in their biophysical properties. TRPM3 is expressed in somatosensory neurons and may be important in development of heat hyperalgesia during inflammation (see review [803]). TRPM3 is frequently coexpressed with TRPA1 and TRPV1 in these neurons. TRPM3 is expressed in pancreatic beta cells as well as brain, pituitary gland, eye, kidney, and adipose tissue [614, 802]. TRPM3 may contribute to the detection of noxious heat [870].TRPM2TRPM2 is activated under conditions of oxidative stress (respiratory burst of phagocytic cells) and ischemic conditions. However, the direct activators are ADPR(P) and calcium. As for many ion channels, PIP2 must also be present (reviewed by [935]). Numerous splice variants of TRPM2 exist which differ in their activation mechanisms [200]. The C-terminal domain contains a TRP motif, a coiled-coil region, and an enzymatic NUDT9 homologous domain. TRPM2 appears not to be activated by NAD, NAAD, or NAADP, but is directly activated by ADPRP (adenosine-5'-O-disphosphoribose phosphate) [827]. TRPM2 is involved in warmth sensation [724], and contributes to neurological diseases [61]. Recent study shows that 2'-deoxy-ADPR is an endogenous TRPM2 superagonist [231]. TRPM4/5 subgroupTRPM4 and TRPM5 have the distinction within all TRP channels of being impermeable to Ca2+ [915]. A splice variant of TRPM4 (i.e.TRPM4b) and TRPM5 are molecular candidates for endogenous calcium-activated cation (CAN) channels [278]. TRPM4 is active in the late phase of repolarization of the cardiac ventricular action potential. TRPM4 deletion or knockout enhances beta adrenergic-mediated inotropy [507]. Mutations are associated with conduction defects [347, 507, 753]. TRPM4 has been shown to be an important regulator of Ca2+ entry in to mast cells [847] and dendritic cell migration [39]. TRPM5 in taste receptor cells of the tongue appears essential for the transduction of sweet, amino acid and bitter stimuli [460] TRPM5 contributes to the slow afterdepolarization of layer 5 neurons in mouse prefrontal cortex [439]. Both TRPM4 and TRPM5 are required transduction of taste stimuli [206].TRPM6/7 subgroupTRPM6 and 7 combine channel and enzymatic activities (‘chanzymes’). These channels have the unusual property of permeation by divalent (Ca2+, Mg2+, Zn2+) and monovalent cations, high single channel conductances, but overall extremely small inward conductance when expressed to the plasma membrane. They are inhibited by internal Mg2+ at ~0.6 mM, around the free level of Mg2+ in cells. Whether they contribute to Mg2+ homeostasis is a contentious issue. When either gene is deleted in mice, the result is embryonic lethality. The C-terminal kinase region is cleaved under unknown stimuli, and the kinase phosphorylates nuclear histones. TRPM7 is responsible for oxidant- induced Zn2+ release from intracellular vesicles [3] and contributes to intestinal mineral absorption essential for postnatal survival [532]. TRPM8Is a channel activated by cooling and pharmacological agents evoking a ‘cool’ sensation and participates in the thermosensation of cold temperatures [50, 147, 186] reviewed by [864, 481, 391, 556]. TRPML (mucolipin) familyThe TRPML family [676, 964, 670, 926, 156] consists of three mammalian members (TRPML1-3). TRPML channels are probably restricted to intracellular vesicles and mutations in the gene (MCOLN1) encoding TRPML1 (mucolipin-1) cause the neurodegenerative disorder mucolipidosis type IV (MLIV) in man. TRPML1 is a cation selective ion channel that is important for sorting/transport of endosomes in the late endocytotic pathway and specifically, fission from late endosome-lysosome hybrid vesicles and lysosomal exocytosis [704]. TRPML2 and TRPML3 show increased channel activity in low extracellular sodium and are activated by similar small molecules [270]. A naturally occurring gain of function mutation in TRPML3 (i.e. A419P) results in the varitint waddler (Va) mouse phenotype (reviewed by [676, 593]). TRPP (polycystin) familyThe TRPP family (reviewed by [179, 177, 252, 905, 320]) or PKD2 family is comprised of PKD2 (PC2), PKD2L1 (PC2L1), PKD2L2 (PC2L2), which have been renamed TRPP1, TRPP2 and TRPP3, respectively [915]. It should also be noted that the nomenclature of PC2 was TRPP2 in old literature. However, PC2 has been uniformed to be called TRPP2 [293]. PKD2 family channels are clearly distinct from the PKD1 family, whose function is unknown. PKD1 and PKD2 form a hetero-oligomeric complex with a 1:3 ratio. [775]. Although still being sorted out, TRPP family members appear to be 6TM spanning nonselective cation channels. TRPV (vanilloid) familyMembers of the TRPV family (reviewed by [849]) can broadly be divided into the non-selective cation channels, TRPV1-4 and the more calcium selective channels TRPV5 and TRPV6.TRPV1-V4 subfamilyTRPV1 is involved in the development of thermal hyperalgesia following inflammation and may contribute to the detection of noxius heat (reviewed by [660, 756, 786]). Numerous splice variants of TRPV1 have been described, some of which modulate the activity of TRPV1, or act in a dominant negative manner when co-expressed with TRPV1 [722]. The pharmacology of TRPV1 channels is discussed in detail in [280] and [868]. TRPV2 is probably not a thermosensor in man [635], but has recently been implicated in innate immunity [469]. TRPV3 and TRPV4 are both thermosensitive. There are claims that TRPV4 is also mechanosensitive, but this has not been established to be within a physiological range in a native environment [106, 454].TRPV5/V6 subfamily TRPV5 and TRPV6 are highly expressed in placenta, bone, and kidney. Under physiological conditions, TRPV5 and TRPV6 are calcium selective channels involved in the absorption and reabsorption of calcium across intestinal and kidney tubule epithelia (reviewed by [901, 168, 558, 227]).

The TRP superfamily of channels (nomenclature as agreed by NC-IUPHAR [159, 999]), whose founder member is the Drosophila Trp channel, exists in mammals as six families; TRPC, TRPM, TRPV, TRPA, TRPP and TRPML based on amino acid homologies. TRP subunits contain six putative TM domains and assemble as homo- or hetero-tetramers to form cation selective channels with diverse modes of activation and varied permeation properties (reviewed by [679]). Established, or potential, physiological functions of the individual members of the TRP families are discussed in detail in the recommended reviews and in a number of books [371, 635, 1066, 236]. The established, or potential, involvement of TRP channels in disease is reviewed in [412, 634] and [637], together with a special edition of Biochemica et Biophysica Acta on the subject [634]. Additional disease related reviews, for pain [585], stroke [1052], sensation and inflammation [921], itch [117], and airway disease [284, 979], are available. The pharmacology of most TRP channels has been advanced in recent years. Broad spectrum agents are listed in the tables along with more selective, or recently recognised, ligands that are flagged by the inclusion of a primary reference. See Rubaiy (2019) for a review of pharmacological tools for TRPC1/C4/C5 channels [751]. Most TRP channels are regulated by phosphoinostides such as PtIns(4,5)P2 although the effects reported are often complex, occasionally contradictory, and likely to be dependent upon experimental conditions, such as intracellular ATP levels (reviewed by [941, 638, 747]). Such regulation is generally not included in the tables.When thermosensitivity is mentioned, it refers specifically to a high Q10 of gating, often in the range of 10-30, but does not necessarily imply that the channel's function is to act as a 'hot' or 'cold' sensor. In general, the search for TRP activators has led to many claims for temperature sensing, mechanosensation, and lipid sensing. All proteins are of course sensitive to energies of binding, mechanical force, and temperature, but the issue is whether the proposed input is within a physiologically relevant range resulting in a response. TRPA (ankyrin) familyTRPA1 is the sole mammalian member of this group (reviewed by [268]). TRPA1 activation of sensory neurons contribute to nociception [382, 831, 555]. Pungent chemicals such as mustard oil (AITC), allicin, and cinnamaldehyde activate TRPA1 by modification of free thiol groups of cysteine side chains, especially those located in its amino terminus [529, 51, 336, 531]. Alkenals with α, β-unsaturated bonds, such as propenal (acrolein), butenal (crotylaldehyde), and 2-pentenal can react with free thiols via Michael addition and can activate TRPA1. However, potency appears to weaken as carbon chain length increases [23, 51]. Covalent modification leads to sustained activation of TRPA1. Chemicals including carvacrol, menthol, and local anesthetics reversibly activate TRPA1 by non-covalent binding [391, 470, 1007, 1006]. TRPA1 is not mechanosensitive under physiological conditions, but can be activated by cold temperatures [392, 193]. The electron cryo-EM structure of TRPA1 [688] indicates that it is a 6-TM homotetramer. Each subunit of the channel contains two short ‘pore helices’ pointing into the ion selectivity filter, which is big enough to allow permeation of partially hydrated Ca2+ ions. TRPC (canonical) familyMembers of the TRPC subfamily (reviewed by [261, 726, 15, 4, 84, 410, 687, 60]) fall into the subgroups outlined below. TRPC2 is a pseudogene in humans. It is generally accepted that all TRPC channels are activated downstream of Gq/11-coupled receptors, or receptor tyrosine kinases (reviewed by [713, 889, 999]). A comprehensive listing of G-protein coupled receptors that activate TRPC channels is given in [4]. Hetero-oligomeric complexes of TRPC channels and their association with proteins to form signalling complexes are detailed in [15] and [411]. TRPC channels have frequently been proposed to act as store-operated channels (SOCs) (or compenents of mulimeric complexes that form SOCs), activated by depletion of intracellular calcium stores (reviewed by [689, 15, 718, 765, 1039, 141, 675, 55, 142]). However, the weight of the evidence is that they are not directly gated by conventional store-operated mechanisms, as established for Stim-gated Orai channels. TRPC channels are not mechanically gated in physiologically relevant ranges of force. All members of the TRPC family are blocked by 2-APB and SKF96365 [319, 318]. Activation of TRPC channels by lipids is discussed by [60]. Important progress has been recently made in TRPC pharmacology [751, 571, 400, 92]. TRPC channels regulate a variety of physiological functions and are implicated in many human diseases [270, 61, 827, 960]. TRPC1/C4/C5 subgroup TRPC1 alone may not form a functional ion channel [210]. TRPC4/C5 may be distinguished from other TRP channels by their potentiation by micromolar concentrations of La3+. TRPC2 is a pseudogene in humans, but in other mammals appears to be an ion channel localized to microvilli of the vomeronasal organ. It is required for normal sexual behavior in response to pheromones in mice. It may also function in the main olfactory epithelia in mice [1036, 672, 673, 1037, 496, 1077, 1032].TRPC3/C6/C7 subgroup All members are activated by diacylglycerol independent of protein kinase C stimulation [319].TRPM (melastatin) familyMembers of the TRPM subfamily (reviewed by [252, 318, 689, 1064]) fall into the five subgroups outlined below. TRPM1/M3 subgroupIn darkness, glutamate released by the photoreceptors and ON-bipolar cells binds to the metabotropic glutamate receptor 6 , leading to activation of Go . This results in the closure of TRPM1. When the photoreceptors are stimulated by light, glutamate release is reduced, and TRPM1 channels are more active, resulting in cell membrane depolarization. Human TRPM1 mutations are associated with congenital stationary night blindness (CSNB), whose patients lack rod function. TRPM1 is also found melanocytes. Isoforms of TRPM1 may present in melanocytes, melanoma, brain, and retina. In melanoma cells, TRPM1 is prevalent in highly dynamic intracellular vesicular structures [368, 657]. TRPM3 (reviewed by [663]) exists as multiple splice variants which differ significantly in their biophysical properties. TRPM3 is expressed in somatosensory neurons and may be important in development of heat hyperalgesia during inflammation (see review [878]). TRPM3 is frequently coexpressed with TRPA1 and TRPV1 in these neurons. TRPM3 is expressed in pancreatic beta cells as well as brain, pituitary gland, eye, kidney, and adipose tissue [662, 877]. TRPM3 may contribute to the detection of noxious heat [949].TRPM2TRPM2 is activated under conditions of oxidative stress (respiratory burst of phagocytic cells) and ischemic conditions. However, the direct activators are ADPR(P) and calcium. As for many ion channels, PIP2 must also be present (reviewed by [1020]). Numerous splice variants of TRPM2 exist which differ in their activation mechanisms [219]. The C-terminal domain contains a TRP motif, a coiled-coil region, and an enzymatic NUDT9 homologous domain. TRPM2 appears not to be activated by NAD, NAAD, or NAADP, but is directly activated by ADPRP (adenosine-5'-O-disphosphoribose phosphate) [902]. TRPM2 is involved in warmth sensation [789], and contributes to neurological diseases [66]. Recent study shows that 2'-deoxy-ADPR is an endogenous TRPM2 superagonist [253]. TRPM4/5 subgroupTRPM4 and TRPM5 have the distinction within all TRP channels of being impermeable to Ca2+ [999]. A splice variant of TRPM4 (i.e.TRPM4b) and TRPM5 are molecular candidates for endogenous calcium-activated cation (CAN) channels [301]. TRPM4 is active in the late phase of repolarization of the cardiac ventricular action potential. TRPM4 deletion or knockout enhances beta adrenergic-mediated inotropy [546]. Mutations are associated with conduction defects [374, 546, 821]. TRPM4 has been shown to be an important regulator of Ca2+ entry in to mast cells [926] and dendritic cell migration [43]. TRPM5 in taste receptor cells of the tongue appears essential for the transduction of sweet, amino acid and bitter stimuli [494] TRPM5 contributes to the slow afterdepolarization of layer 5 neurons in mouse prefrontal cortex [471]. Both TRPM4 and TRPM5 are required transduction of taste stimuli [226].TRPM6/7 subgroupTRPM6 and 7 combine channel and enzymatic activities (‘chanzymes’). These channels have the unusual property of permeation by divalent (Ca2+, Mg2+, Zn2+) and monovalent cations, high single channel conductances, but overall extremely small inward conductance when expressed to the plasma membrane. They are inhibited by internal Mg2+ at ~0.6 mM, around the free level of Mg2+ in cells. Whether they contribute to Mg2+ homeostasis is a contentious issue. When either gene is deleted in mice, the result is embryonic lethality. The C-terminal kinase region is cleaved under unknown stimuli, and the kinase phosphorylates nuclear histones. TRPM7 is responsible for oxidant- induced Zn2+ release from intracellular vesicles [3] and contributes to intestinal mineral absorption essential for postnatal survival [574]. TRPM8Is a channel activated by cooling and pharmacological agents evoking a ‘cool’ sensation and participates in the thermosensation of cold temperatures [54, 161, 205] reviewed by [943, 516, 420, 599]. TRPML (mucolipin) familyThe TRPML family [729, 1049, 723, 1010, 173] consists of three mammalian members (TRPML1-3). TRPML channels are probably restricted to intracellular vesicles and mutations in the gene (MCOLN1) encoding TRPML1 (mucolipin-1) cause the neurodegenerative disorder mucolipidosis type IV (MLIV) in man. TRPML1 is a cation selective ion channel that is important for sorting/transport of endosomes in the late endocytotic pathway and specifically, fission from late endosome-lysosome hybrid vesicles and lysosomal exocytosis [766]. TRPML2 and TRPML3 show increased channel activity in low extracellular sodium and are activated by similar small molecules [293]. A naturally occurring gain of function mutation in TRPML3 (i.e. A419P) results in the varitint waddler (Va) mouse phenotype (reviewed by [729, 639]). TRPP (polycystin) familyThe TRPP family (reviewed by [197, 195, 275, 988, 345]) or PKD2 family is comprised of PKD2 (PC2), PKD2L1 (PC2L1), PKD2L2 (PC2L2), which have been renamed TRPP1, TRPP2 and TRPP3, respectively [999]. It should also be noted that the nomenclature of PC2 was TRPP2 in old literature. However, PC2 has been uniformed to be called TRPP2 [317]. PKD2 family channels are clearly distinct from the PKD1 family, whose function is unknown. PKD1 and PKD2 form a hetero-oligomeric complex with a 1:3 ratio. [845]. Although still being sorted out, TRPP family members appear to be 6TM spanning nonselective cation channels. TRPV (vanilloid) familyMembers of the TRPV family (reviewed by [928]) can broadly be divided into the non-selective cation channels, TRPV1-4 and the more calcium selective channels TRPV5 and TRPV6.TRPV1-V4 subfamilyTRPV1 is involved in the development of thermal hyperalgesia following inflammation and may contribute to the detection of noxius heat (reviewed by [710, 824, 860]). Numerous splice variants of TRPV1 have been described, some of which modulate the activity of TRPV1, or act in a dominant negative manner when co-expressed with TRPV1 [787]. The pharmacology of TRPV1 channels is discussed in detail in [303] and [947]. TRPV2 is probably not a thermosensor in man [684], but has recently been implicated in innate immunity [503]. TRPV3 and TRPV4 are both thermosensitive. There are claims that TRPV4 is also mechanosensitive, but this has not been established to be within a physiological range in a native environment [114, 488].TRPV5/V6 subfamily TRPV5 and TRPV6 are highly expressed in placenta, bone, and kidney. Under physiological conditions, TRPV5 and TRPV6 are calcium selective channels involved in the absorption and reabsorption of calcium across intestinal and kidney tubule epithelia (reviewed by [984, 185, 601, 248]).

Les phases alpha'-Fe8N1-x et alpha''-Fe16N2 ont un fort potentiel d’application, en raison de leur anisotropie magnétocristalline uniaxiale et de leur grande aimantation à saturation. Cependant, les valeurs annoncées pour ces propriétés magnétiques restent sujettes à discussion. Les recherches menées au cours de cette thèse de doctorat ont été initiées dans le but de clarifier cette situation. L’élaboration des échantillons a principalement consisté en l’implantation ionique d’azote dans des couches minces de fer alpha épitaxiées sur ZnSe/GaAs (001). Entre autres, les effets de la température de la cible et de la fluence sur la structure cristalline des échantillons ont été analysés par diffractométrie des rayons X. La présence d’une anisotropie magnétique perpendiculaire a été mise en évidence dans les couches minces contenant les phases alpha'-Fe8N1-x ou alpha''-Fe16N2. La constante d’anisotropie a été évaluée par magnétométrie à échantillon vibrant et résonance ferromagnétique. À l’occasion de ces recherches, des domaines en rubans faibles ont été observés par microscopie à force magnétique dans certaines couches minces en Fe-N. Ceux-ci sont particulièrement rectilignes et des dislocations coin se trouvent au sein de leur structure périodique. Des études ont alors été réalisées dans le but de contrôler avec précision la réorientation des domaines en rubans et le déplacement des dislocations magnétiques, à l’aide d’un champ magnétique
The alpha'-Fe8N1-x and alpha''-Fe16N2 phases have a high potential of application, because of their uniaxial magnetocrystalline anisotropy and their large saturation magnetization. However, the values announced for these magnetic properties remain a subject of discussion. The research conducted during this PhD thesis was initiated in order to clarify this situation. Sample making consisted mainly of nitrogen ion implantation into alpha-Fe thin films, epitaxially grown on ZnSe/GaAs (001). Among others, the effects of target temperature and fluence on the crystal structure of the samples were analyzed by X-ray diffractometry. The presence of a perpendicular magnetic anisotropy was demonstrated in the thin films containing the alpha'-Fe8N1-x and alpha''-Fe16N2 phases. The anisotropy constant was evaluated by vibrating sample magnetometry and ferromagnetic resonance. In this research, weak stripe domains were observed by magnetic force microscopy in some Fe-N thin films. These are particularly straight and edge dislocations are found within their periodic structure. Studies were then carried out to precisely control the reorientation of the stripe domains and the displacement of the magnetic dislocations, using a magnetic field

Des distributions inhomogènes de l’aimantation existent lorsque le matériau n’est pas uniforme, ou lorsqu’une texture magnétique se forme dans un matériau homogène. Dans les deux cas, les symétries brisées modifient l’excitation et la propagation des ondes de spin et donnent lieu à des phénomènes surprenants. Dans ce contexte, nous avons étudié la propagation des ondes de spin dans une bicouche avec un contraste de l’aimantation de saturation dans la configuration Damon-Eshbach. Nous avons trouvé, à l’aide de simulations et expériences (spectroscopie d'ondes de spin propagatives et diffusion de Brouillon), que le système montre une très forte non-réciprocité en fréquence qui peut être utilisée pour réaliser une diode magnonique. Par ailleurs, nous avons étudié la dynamique des ondes de spin dans des couches minces qui présentent des domaines magnétiques en rubans faibles. Nous avons montré que ces ondes de spin peuvent être interprétées comme une extension des modes de Damon-Eshbach dans l’état saturé, qui s’adaptent à la brisure de symétrie. Nous avons également montré que les deux modes d’ondes de spin de plus basse fréquence correspondent aux modes de Goldstone et Higgs de la texture en rubans. Ces résultats ont été confirmés par des mesures de diffusion Brillouin et de résonance ferromagnétique
Inhomogeneous magnetization distributions may exist because the magnetic parameters are distributed, or because magnetic textures nucleate in homogenous materials. In both cases, the broken symmetries affect the spin-wave excitation and propagation, leading to a number of intriguing phenomena. In this context, we have studied the propagation of spin waves in a bilayer with a saturation magnetization contrast for the Damon-Eshbach configuration. We have found, by means of simulations and experiments (Propagating Spin Wave Spectroscopy and Brillouin Light Scattering), that this system shows a strong frequency non-reciprocity which can be used for the realization of a spin-wave diode. We have also studied the spin-wave dynamics in thin films which exhibit weak magnetic stripe domains. We have shown how these modes can be interpreted as an extension of the Damon-Eshbach spectrum of the saturated state, which adapts to the symmetry breaking. Furthermore, we have identified the two lowest frequency modes to the Goldstone- and Higgs- modes of the stripe texture. These results were confirmed by Brillouin Light Scattering and Ferromagnetic Resonance experiments

Nous presentons une etude sur les materiaux magnetiques doux a anisotropie evanescente, et plus particulierement sur les materiaux nanocristallins. Cette etude se decompose en deux parties. La premiere partie, une approche numerique basee sur l'integration de l'equation de gilbert landau et lifshitz, permet l'etude du modele a anisotropie aleatoire a une dimension, applique aux materiaux nanocristallins. Ces simulations micromagnetiques nous permettent de comprendre les mecanismes d'aimantation mis en jeu et mettent en evidence la presence de nuds de parois qui sont lies a la topologie 1d du systeme. Dans la deuxieme partie de cette these, nous nous interessons a la dynamique des parois de bloch dans les materiaux nanocristallins doux. Nous observons experimentalement la multiplication des parois pour un champ critique. Ce champ critique permet d'approcher la valeur de l'energie surfacique des parois et donc de remonter a l'anisotropie vue par celles-ci.

Nous proposons dans cette thèse de nouvelles structures à faibles pertes en vue de leur utilisation dans les bandes millimétriques. L'hypothèse forte sur laquelle es t construite notre étude est que la présence du silicium permet d'envisager une intégration plus facile des circuits. Pour ce faire nous avons étudié les caractéristiques électriques des lignes inversées silicium-verre à 60 GHz ainsi que la ligne de Goubau sur silicium aux mêmes fréquences. Nous nous sommes focalisés sur l'étude des pertes des lignes. Les simulations présentées jusqu'à 60 GHz ont montré que les qualités de la technologie verre-silicium sont bonnes en termes de paramètres hyperfréquences. Nous proposons donc d'utiliser plutôt le verre pour la partie passive et le silicium pour la partie active. Nous montrons ainsi que cette technologie admet la réalisation des fonctions diverses et qu'elle est susceptible de permettre l'obtention de systèmes intégrés

In this thesis we present solutions of spontaneous compactifications of D=11, N=1 supergravity on parallelizable manifolds S^1, S^3 and S^7. In Freund-Rubin compactifications one usually obtains AdS vacua in 4D, these solutions usually sets the fermionic VEV's to zero. However giving them non zero VEV's allows us to define torsion given by the fermionic bilinears that essentially flattens the geometry giving us a vanishing cosmological constant on M_4. We further give an analysis of the consistent truncation of the bosonic sector of D=11 supergravity on a S^3 manifold and relate this to other known consistent truncation compactifications. We also consider the squashed S^7 where we check for surviving supersymmetries by analyzing the generalised holonomy, this compactification is of interest in phenomenology.

Obtention par voie électrolytique de couches minces de Ni-P amorphe. Obtention de façon reproductible de rubans de grandes dimensions. Mesure de résistivité électrique sur des rubans de différentes compositions. Détermination de la conductivité et de la diffusivité thermiques de l'alliage Ni-P à partir de la propagation de la chaleur dans des rubans de cet alliage. Réalisation d'un banc de mesure fournissant en temps réel les variations thermiques dans le ruban. Stockage des données et tracé des courbes effectué à l'aide d'un microordinateur. Vérification de la loi Wiedemann Franz

The ‘Introduction’ asks what is ritual? Is ritual useful? What are the various kinds of ritual? It suggests that to think about ritual is to reflect on human nature, sociality, and culture. It is also to explore ritual's place, power, and potential in our lives and our society. Ritual includes both religious and nonreligious rites, the traditional and the new, the prescribed and the improvised, the human and nonhuman, and rubs up against a number of other cultural domains, such as play, games, performance, and theater. If ritual is action, it is also an idea, something we think with, and our exploration will move back and forth between these two dimensions.

In this chapter we explore the science of “external” remedies in India. What are some of the ways that people in India treat the body and mind and seek overall wellbeing through ornamentation and surface application of gemstones (maṇi) and amulets (bhadrikā and rakṣikā) (vis-à-vis, for example, “internal” treatments involving botanical decoctions, oils and powders that are assimilated by the body)? Drawing on textual references and ethnographic examples, we discuss how gemstones (e.g., rubies, pearls and sapphires) and amulets (constructed from such things as devil tree, wild asparagus, seashells, and other substances) have been used in India as preventative therapies in a person’s daily regimen and as specific remedies for disorders during pregnancy, planetary-based afflictions, and madness, among other things. The preparations, testing, and practices of minerals and amulets that are used to treat the body and mind reveal a worldview in which the human person is entwined within multiple and intersecting ecosystems. It is a worldview, furthermore, in which notions of bodily externality and internality are blurred. A distinctive and wide-ranging notion of wellbeing emerges, we suggest, that connects the mineral, botanical, and astral domains with the human body’s functioning and a person’s course of life.

The dynamic behavior of rotating machines due to faults can be simulated with model-based methods in which the faults are modelled with suitable sets of equivalent excitations. When rotor-to-stator rubs occur the friction forces can cause time-varying thermal bows of the shafts. The equivalent bending moments that cause the shaft bows can be estimated with model-based identification methods developed in the frequency domain: these techniques minimize the error between the machine experimental vibrations induced by the rubs and the system response provided by the simulating model. In this paper the results of the analysis of the experimental vibrations of a large turbine-generator unit that was subjected to partial-arc rubs are shown and discussed along with the diagnostic strategy used to identify the fault severity and the location of the shaft cross-sections where the heaviest rubs occurred. Comparisons between experimental data and simulated vibrations caused by the identified fault are shown.

Abstract In gas-turbine engines and other rotating machinery structures rubbing contact interactions can occur when the contacting components have large relative motion between components: such as in rotating bladed disc-casing rubbing contacts, rubbing in rotor bearing and labyrinth seals, etc. The analysis of vibrations of structures with rubbing contacts requires the development of a mathematical model and special friction contact elements that would allow for the prescribed relative motion of rubbing surfaces in addition to the motion due to vibrations of the contacting components. In the proposed paper, the formulation of the friction contact elements is developed which includes the effects of the prescribed relative motion on the friction stick-slip transitions and, therefore, on the contact interaction forces. For a first time, the formulation is made for the frequency domain analysis of coupled rubbing and vibrational motion, using the multiharmonic representation of the vibration displacements. The formulation is made fully analytically to express the multiharmonic contact interaction forces and multiharmonic tangent stiffness matrix in an explicit analytical form allowing their calculation accurately and fast. The dependency of the friction and contact stiffness coefficients on the energy dissipated during high-energy rubbing contacts and, hence, on the corresponding increase of the contact interface temperature is included in the formulation. The efficiency of the developed friction elements is demonstrated on a set of test cases including simple models and a large-scale realistic blade.

The dynamic forced response of a two-degrees-of-freedom model of an unbalanced overhung rotor with clearance and symmetric piecewise-linear stiffness is examined in the time domain. The stiffness nonlinearity is representative of the contact between the rotor and a concentric stator ring. This rubbing interaction comes as a result of the rotor transient motion initiated by the sudden application of a static unbalance, such as in a blade loss scenario. The focus of this study is on the range of rotor speeds above resonance, where the contact between rotor and stator is characterised by a “bouncing” or intermittent type of behaviour. Brute-force numerical bifurcation analysis on the long-term forced response revealed ranges of rotation frequency for which there is bi-stability between non-impacting synchronous equilibrium and impacting sub-synchronous motion. It is found that, for sufficiently high levels of transient energy in the rotor, there exists the possibility for the solution to jump into a stable limit cycle characterised by three non-harmonically related frequencies, namely the synchronous response frequency and the forward and backward whirl frequencies. A simple relationship defining the point of synchronisation between these three components is proposed as an explanation to the region of bi-stability detected. The stiffening effect induced by the contact non-linearity enables this synchronisation to be maintained over a range of forcing frequencies rather than just at the single condition determined from the nominal whirl mode frequencies.

Considering rotational speed-dependent stiffness for vibrational analysis of friction-damped bladed disk models has proven to lead to significant improvements in nonlinear frequency response curve computations. The accuracy of the result is driven by a suitable choice of reduction bases. Multi-model reduction combines various bases which are valid for different parameter values. This composition reduces the solution error drastically. The resulting set of equations is typically solved by means of the harmonic balance method. Nonlinear forces are regularized by a Lagrangian approach embedded in an alternating frequency/time domain method providing the Fourier coefficients for the frequency domain solution. The aim of this paper is to expand the multi-model approach to address rotational speed-dependent contact situations. Various reduction bases derived from composing Craig-Bampton, Rubin-Martinez, and hybrid interface methods will be investigated with respect to their applicability to capture the changing contact situation correctly. The methods validity is examined based on small academic examples as well as large-scale industrial blade models. Coherent results show that the multi-model composition works successfully, even if multiple different reduction bases are used per sample point of variable rotational speed. This is an important issue in case that a contact situation for a specific value of the speed is uncertain forcing the algorithm to automatically choose a suitable basis. Additionally, the randomized singular value decomposition is applied to rapidly extract an appropriate multi-model basis. This approach improves the computational performance by orders of magnitude compared to the standard singular value decomposition, while preserving the ability to provide a best rank approximation.

Clearance is of paramount importance for turbomachinery manufacturers to meet today’s aggressive power output, efficiency, and operational life goals. To minimize leakages, there are various seal types used, and new sealing concepts are in development. Because of their inherent flexibility and compliance, brush seals are capable of significantly reducing the leakage, and allow sufficient geometrical margins to accommodate design and operational variations of turbomachines. Brush seals can be assembled at very tight or zero radial clearance or even with interference on the rotor to minimize the leakage. This means that the risk of contact between the rotor and the seal bristles exists, especially in case of zero clearance or interference. If the contact occurs, a hot-spot develops on the rotor and this may cause the vibration to diverge, resulting in a synchronous instability, the so-called Newkirk effect. The friction forces generated by rotor-to-stator rubs often cause a shaft thermal bow whose main effect on the machine dynamic behavior is a progressive change of the synchronous (1X) vibration. The development of analytical tools able to model this phenomenon is therefore important to assess the rotordynamic stability during the design phase and avoid excessive vibrations which may have severe impact on the operability and on the mechanical integrity of the machine. The objective of this paper is the development of a numerical model to analyze the dynamic behavior of real turbomachines subject to thermally-induced vibration caused by light-rub of the rotor against brush seals. The model developed in the paper is based on the work of Bachschmid et al. [1]: the dynamics is analyzed in the frequency domain using the standard rotordynamic model, whereas the heat transfer analysis, to calculate the temperature distribution and the associated thermal bow, is studied in the time domain. The contact analysis has been deeply revised, aiming at estimating suitable normal and tangential force and the friction heating generated by the contact. The bow determined by the thermal conditions has been reproduced using suitable bending moments, which have been applied to the beam elements in the rotordynamic model.