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Дисертації з теми "Drosophila Molecular genetics"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 10 лютого 2022

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1

Johnstone, Oona. "Characterization of the Vasa-eIF5B interaction during Drosophila development." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84265.

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Translational control is an important means of regulating gene expression. Development of the Drosophila germ line relies on translational regulation to differentially express maternal mRNAs, allowing it to develop distinctly from the soma. One of the critical factors required for germ cell development and function is the conserved DEAD-box RNA helicase Vasa (Vas). The research presented in this thesis examines the role of Vas in translational regulation during Drosophila germ line development. A two-hybrid screen conducted with Vas identified a translation initiation factor eIF5B (dIF2

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2

Zhang, Li. "DRMT4 (Drosophila arginine methyltransferase 4) : functions in Drosophila oogenesis." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80905.

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DRMT4 (Drosophila Arginine MethylTransferase 4) is an arginine methyltransferase in Drosophila (Boulanger et al. 2004). It shows the highest identities with mammalian PRMT4/CARM1 (Protein Arginine MethylTransferase 4) (59% identity, 75% similarity). HPLC analysis demonstrated that DRMT4 belongs to the type I class of methyltransferases (Boulanger et al. 2004), meaning that DRMT4 catalyzes asymmetrical dimethylarginine formation. A polyclonal antibody against DRMT4 was generated and used to study DRMT4 expression using western blots and immunostainings. In order to study DRMT4 function

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3

Tauber, Merav. "Molecular genetics of aggressive behaviour in Drosophila melanogaster." Thesis, University of Leicester, 2010. http://hdl.handle.net/2381/10224.

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Aggression is a key component of the normal repertoire of behaviours in a broad range of animals from insects to mammals. Although the genetic basis for aggression is widely accepted, only a few individual candidate genes have been studied. Recent studies have indicated that Drosophila melanogaster can serve as a powerful model system to study the genetics of aggression. The aim of this project was to identify genes associated with aggression by global profiling of the fly transcriptome using DNA expression microarrays. At the core of this study was a behavioural screen in which the aggression

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4

Sun, Qi Zinn Kai George. "Molecular genetics of axon guidance in Drosophila melanogaster /." Diss., Pasadena, Calif. : California Institute of Technology, 2000. http://resolver.caltech.edu/CaltechETD:etd-03242005-130557.

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5

Freeman, Sally Jean. "Molecular analysis of the Drosophila gene, Polyhomeotic." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27924.

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Polyhomeotic (ph) is a developmentally important gene in Drosophila melanogaster which has been genetically characterized and recently cloned. ph is genetically and molecularly complex and has a strong maternal effect. Analysis of null or amorphic alleles reveal phenotypic effects that include embryonic lethality, cell death of the ventral epithelium, homeotic transformations, and alteration in the pattern of axon pathways. Two independent point mutations are required to produce a ph null allele. I have shown that the ph locus contains two, large, highly conserved, tandem repeats that are both

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6

Stevens, Naomi Rosalie. "The molecular regulation of centriole duplication in Drosophila." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611818.

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7

Loh, Samantha Hui Yong. "Molecular and genetic characterisation of Drosophila Sox50E and Sox100B." Thesis, University of Cambridge, 2000. https://www.repository.cam.ac.uk/handle/1810/251700.

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8

Ditch, Lynn Marie. "Molecular genetics of mutations altering sexual behavior in Drosophila /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3071049.

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9

Howard, K. R. "Molecular genetics of the hairy locus of Drosophila melanogaster." Thesis, Imperial College London, 1986. http://hdl.handle.net/10044/1/38040.

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10

Harley, Alyssa Skye. "Analysis of a nuclear role for 'pebble', a gene required for cytokinesis in Drosophila." Title page, abstract and table of contents only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phh284.pdf.

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"May 2002" Bibliography: leaves 157-176. Through the use of a variety of biochemical and genetic techniques, the importance of the nuclear localisation of PBL was examined, as well as the function of its RadECl and BRCT domains. The RadECl/BRCT domains were found to be required in the cytoplasm for cytokinesis, extending the range of function attributed to these domains. PBL was also shown to shuttle between the nucleus and the cytoplasm, providing an explanation for the observed ability of nuclear PBL to influence cytoplasmic structure.

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11

Jud, Molly Christine. "Jun signaling during Drosophila development." Thesis, The University of Utah, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10130207.

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<p> Jun N-terminal kinase (JNK) signaling is a key modulator of development and disease in all multicellular organisms. One process in which the consequences of both gain and loss of JNK signaling can be monitored is embryonic dorsal closure (DC) in the fruit fly, <i>Drosophila melanogaster.</i> DC occurs midway through embryogenesis; it is the process by which the lateral epidermis expands bilaterally to meet and fuse at the dorsal midline, thereby encasing the entire embryo in epidermis. JNK signaling in leading edge (LE) cells (the dorsal-most row of epidermis) initiates closure. My studies

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12

Crompton, Douglas Ewan. "Molecular analysis of the shaking-B locus of Drosophila melanogaster." Thesis, University of Glasgow, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309523.

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13

Abukashawa, Sumaia. "Patterns of molecular evolution at the amylase locus in Drosophila." Thesis, University of Ottawa (Canada), 1990. http://hdl.handle.net/10393/5995.

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Genes encoding the starch-degrading enzyme, alpha-amylase, are found in all major groups of animals, plants and microbes. In this thesis, amylase-coding sequences have been chosen as a model system to investigate the patterns of molecular evolution in an enzyme-coding gene. Previous phylogenetic comparisons of amylase-coding sequences have shown high levels of primary sequence conservation over long evolutionary periods. The studies described here have concentrated on the evolution of these genes within the genus Drosophila. I have studied patterns of genetic variation within populations of a

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14

Carneiro, da Silva Joana Servulo Correia. "Population genetics of P transposable elements and their host species, with emphasis on Drosophila willistoni and Drosophila sturtevanti." Diss., The University of Arizona, 2000. http://hdl.handle.net/10150/284221.

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The evolution of the P element family was studied in members of the Drosophila willistoni and Drosophila saltans species groups (subgenus Sophophora). The transmission of P elements among species, their spread within species and the strength of selective constraints, as well as the level at which they are imposed on these elements, were investigated using DNA sequence data. Particular emphasis was placed on the evolution of the canonical P element subfamily. This subfamily includes the functional P element first isolated from Drosophila melanogaster, which was termed canonical. It includes a

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15

Chicoine, Jarred. "The molecular role of Bicaudal-C in Drosophila oogenesis /." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102968.

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Bicaudal-C (Bic-C) encodes a KH-type RNA binding protein required maternally for anterior patterning of the Drosophila oocyte and correct migration of the centripetal follicle cells. In Drosophila, premature translation of the germ-plasm determinant Oskar in Bic-C mutant oocytes suggests a function for Bic-C in post-transcriptional gene regulation.<br>Purification and microarray analysis of Bic-C containing ribonucleoprotein complexes revealed that Bic-C associates with multiple transcripts encoding functionally-related components of the Wnt/Frizzled/Dishevelled signaling pathway that regulate

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16

Hu, Song. "The molecular genetics of the raspberry locus in Drosophila melanogaster." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ34780.pdf.

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17

McCaffrey, Ruth Anne. "Molecular and genetic characterisation of spindle-C in Drosophila melanogaster." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621898.

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18

O'Connell, Sinead. "Functional characterisation of the Polycomblike protein of Drosophila melanogaster." Title page, table of contents and abstract only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09pho1841.pdf.

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19

Corish, Peter. "Molecular studies of non-cytotype P-M hybrid dysgenesis repression in Drosophila melanogaster." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624105.

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20

Lonie, Andrew. "Cloning and characterisation of the Polycomblike gene, a transacting repressor of homeotic gene expression in Drosophila." Title page, contents and summary only, 1994. http://hdl.handle.net/2440/21504.

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Includes bibliographies.<br>{59} leaves : ill. ; 30 cm.<br>Title page, contents and abstract only. The complete thesis in print form is available from the University Library.<br>The Polycomblike gene of Drosophila melanogaster is required for the correct spatial expression of the homeotic genes of Antenapaedia and Bithorax Complexes. This thesis describes the isolation and molecular characterization of the Polycomblike gene.<br>Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1995

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21

Lavoie, Cynthia. "The molecular and biochemical characterization of proteins involved in translation initiation in Drosophila melanogaster." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29072.

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A preliminary analysis of translation initiation has been carried out using the model system Drosophila melanogaster. These efforts have focussed on identifying the homolog of mammalian of mammalian eIF-4F, a complex of three subunits; eIF-4E which binds the mRNA cap, eIF-4A which has ATP-dependent RNA helicase activity, and eIF-4$ gamma$, of unknown function. Attempts to clone the genes encoding subunits of eIF-4F have led to the isolation of two novel genes. A molecular screen for members of the DEAD family of RNA helicases that includes eIF-4A led to the isolation of a gene which encodes th

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22

Jeffs, Pete. "The molecular evolution of the Adh gene in the Drosophila melanogaster subgroup." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333273.

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23

Saunders, Robert David Comrie. "Molecular analysis of a female-sterile mutation in Drosophila melanogaster." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/12900.

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24

Nguyen, Thuy 1973. "Identification of factors which interact with Bicaudal-D in oocyte determination." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=20598.

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Traditional screens for female sterile mutants have revealed only two genes which when mutant, give a fully penetrant 16 nurse cell phenotype. One way to gain a better understanding of the function of these genes in oocyte determination, is to identify genes which interact with them. Using P-element mutagenesis, I have isolated one dominant suppressor and five dominant enhancers of Bicaudal-D, and begun the phenotypic and molecular characterization of three of these genes. By deficiency screening, I have identified two different loci which act as dominant enhancers of Bic-D, and eight differen

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25

Rother, Katherine L. "The KH domain protein BICAUDAL-C regulates oskar expression during Drosophila mid-oogenesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0005/MQ44261.pdf.

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26

Paré, Chantal. "Genetic analysis of localization of a Bic-D::GFP fusion protein and identification of novel subcellular domains." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0028/MQ50851.pdf.

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27

Weston, Matthew James Dominic. "The genetic and molecular characterisation of weak localiser : a gene required for oskar mRNA localisation in Drosophila." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627603.

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28

Torres, Isabel Laureano. "The molecular function of Drosophila PAR-1 in the establishment of cell polarity and microtubule cytoskeleton regulation." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612544.

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29

Hain, Daniel. "Genetic and molecular analysis of drop out, the single homolog of the vertebrate MAST kinases in Drosophila melanogaster." Thesis, University of Dundee, 2011. https://discovery.dundee.ac.uk/en/studentTheses/4d371f0c-df4c-41de-bc5b-3f2e8ee5d35a.

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Cellularisation is a specialised form of cytokinesis in Drosophila melanogaster. Cellularisation occurs after the first 13 syncytial cell cycles of the embryo and involves targeted insertion of membrane to form the blastoderm, which represents a polarised epithelium made out of about 6000 cells. The molecular machinery driving cellularisation is complex and not well understood. In this work a novel gene regulating this process is identified and characterised. The mutation drop out causes defects in intracellular transport, cell polarity and nuclear positioning. Previous work provided evidence

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30

Ragab, Anan. "Genetic and functional studies on abundant Drosophila HMGB proteins." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.615068.

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31

Jowdy, Casey C. "The Regulation of Commissureless in the Embryonic CNS of Drosophila melanogaster." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1284172382.

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32

McElroy, Kyle A. "Balancing transcriptional activity in Drosophila through protein-protein interactions on chromatin." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493492.

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Chromatin plays a vital role in the implementation of gene expression programs. Several disparate groups of regulatory proteins alter chromatin state through post-translational modification of histone proteins, nucleosome remodeling, and higher order chromatin structure in order to affect gene expression. Several of these key groups, such as the Male-Specific Lethal complex and Polycomb Group have been well characterized in Drosophila. Yet aspects of their biology at the molecular level, such as the means by which they are faithfully targeted to regulated loci throughout the genome and the

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33

Eleiche, Aliaa Abdel-Salam. "Developing an eggshell marker based on a dominant female sterile mutation for the identification of complete follicle cell clones in Drosophila melanogaster." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101118.

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Patterning of the body axes of the Drosophila embryo depends on maternally expressed genes, some of which function in the follicular epithelium of the developing egg chamber. Many such genes were identified in genetic screens for homozygous mutant females that produce abnormal embryos. However, mutations in zygotically required maternal effect genes are homozygous lethal, and therefore viable females cannot be recovered using this screening approach. This limitation can be overcome by generating homozygous mutant follicle cell clones in heterozygous females using a system that induces site-spe

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34

Swan, Andrew. "Initiation of developmental asymmetry by Drosophila Bic-D, DLis-1 and microtubules." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0021/NQ55384.pdf.

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35

Ballard, Shannon L. "Regulation of Drosophila larval growth and metabolism by BMP signaling." View abstract/electronic edition; access limited to Brown University users, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3318289.

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36

Morais, de Sá Eurico Manuel. "Molecular mechanisms for organization of cell polarity and axis formation in Drosophila." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608497.

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37

Sarac, Amila. "Investigating pellino function in Drosophila development." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100208.

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Although many of the genes and pathways involved in Drosophila embryogenesis have been thoroughly investigated, a complete understanding of the mechanisms behind these processes is still lacking. In order to gain a better perspective, the main objective of current research is to identify additional components of the signaling pathways that are crucial for normal Drosophila development.<br>One such developmental process is germ band retraction, which occurs in mid-embryogenesis and consists of the movement of the tail end of the germ band, or embryo proper, to its final posterior position. One

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38

Martin, Sophie Geneviève. "Molecular and genetic analysis of cell polarisation, mRNA localisation and axis formation during Drosophila oogenesis." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620714.

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39

Rounding, Atkey Matthew. "Follicle cell fate determination in the Drosophila ovary : the role of the capicua gene." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84068.

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The gene capicua is required for the establishment of dorsal-ventral polarity in the Drosophila melanogaster ovary. Loss of capicua function in the follicle cells results in dorsalization of both the embryo and eggshell. The most prominent dorsal features of the Drosophila eggshell are the dorsal appendages. We show that loss of capicua function results in the ventral ectopic specification of dorsal appendage-producing follicle cell fate. This cell fate change is due in part to the ectopic expression of genes such as mirror and Broad-Complex in capicua mutant ovaries. When either mirror

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40

Mosley-Bishop, Kathleen Laverne. "Molecular genetic analysis of the Klarsicht gene in Drosophila eye development /." Digital version accessible at:, 1998. http://wwwlib.umi.com/cr/utexas/main.

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41

Sinadinos, Christopher. "Analysis of axonal transport and molecular chaperones during neurodegeneration in Drosophila." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/183403/.

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Neuronal dysfunction and cell death occurs during neurodegeneration. Animal models that express human disease genes and show neurodegenerative-like pathologies are widely used to study particular molecular systems in early neurodegenerative changes. Axonal transport (AT) is perturbed in several prevalent neurodegenerative diseases. The development of a Huntington’s Disease (HD) model in Drosophila melanogaster larvae is described, in which disease gene expression is directed to motor neurons (Chapter 2). This results in stalling and accumulation of AT vesicles in live animals and a locomotion

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42

An, Xin. "Behavioural and molecular studies of the Drosophila brain using the P[GAL4] enhancer-trap." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363158.

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43

Weinzierl, R. O. J. "Molecular studies of the ultrabithorax gene in Drosophila and of homologous genes in vertebrates." Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233603.

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44

Jacobs, Marc D. "The molecular evolution of the alcohol dehydrogenase gene in the Drosophila melanogaster species group." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338323.

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45

Mahone, Michèle. "The phenotypic and molecular characterization of the Bicaudal-C locus in Drosophila melanogaster." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22766.

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Bicaudal-C is a dominant maternal effect mutation which shows incomplete penetrance. Females are fertile and not all their progeny are affected. Those embryos which do not hatch show defects in their antero-posterior polarity, and give rise to bicaudal embryos which are duplicated for posterior structures. Twelve alleles of the genes have been phenotypically analysed. The penetrance of each allele has been determined and the phenotypes of embryonic defects such as mouth/head defect, bicaudal and uncellularized embryos classified and scored, in order to use this information to analyse these all

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46

Masrouha, Nisrine. "Functional analysis of the Drosophila chk2 gene, loki : analysis of novel genetic interactors of Bic-D in Drosophila melanogaster." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80329.

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Cell cycle checkpoints are signal transduction pathways that control the order and timing of cell cycle transitions, ensuring that critical events are completed before the cell cycle proceeds. The Chk2 family of kinases plays a central role in mediating responses to DNA damage or DNA replication blocks in various organisms. My functional analysis of the Drosophila serine/threonine kinase Loki/Chk2 shows that fly chk2 monitors double-strand breaks caused by irradiation during S and G2 phases and induces cell cycle arrest in embryonic cells around cellularization.<br>loki is also required

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47

Wesolowska, Natalia. "Modification and nuclear organization of the Drosophila melanogaster genome." Thesis, The Johns Hopkins University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3575013.

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<p>The success of Drosophila as a system for genetic analysis is closely linked to its amenability to genetic manipulation. Part 1 of the dissertation elucidates a novel scheme for long-range targeted manipulation of genes. We integrated an 80-kb genomic fragment at its endogenous locus, utilizing a targeted attP attachment site for the phiC31 integrase. We achieved single-copy reduction of the resulting region duplication by inducing recombinational DNA repair. We showed that this two-step scheme of integration and reduction is efficient and useful for delivering modifications. We established

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48

Barkus, Rosemarie Victoria. "The functions of Unc-104 in Drosophila motor axons." [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3278203.

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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2007.<br>Title from home page (viewed Nov. 14, 2008). Source: Dissertation Abstracts International, Volume: 68-09, Section: B, page: 5708. Adviser: William Saxton.

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Patarro, Thais de França. "Produtividade em espécies de Drosophila do subgrupo saltans (grupo saltans, subgênero Sophophora) : efeitos da infecção por Wolbachia em linhagens normais e introgredidas /." São José do Rio Preto, 2015. http://hdl.handle.net/11449/127784.

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Orientador: Hermione Elly Melara de Campos Bicudo<br>Banca: Carlos Roberto Ceron<br>Banca: Marluci Monteiro Guirado<br>Banca: Maurício Roberto Viana Sant'Anna<br>Banca: Fabio de Melo Sene<br>Resumo: Mecanismos de isolamento reprodutivo são agentes que impedem ou diminuem a troca de genes entre duas espécies ou populações de uma mesma espécie que se encontram em processo de especiação. Esses mecanismos são compreendidos por uma serie de processos que atuam em diferentes níveis da reprodução, incluindo desde barreiras pré-zigóticas até barreiras pós-zigóticas. Nas ultimas décadas, muitos estudos

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Yang, Long 1976. "Functional analysis of the loki serinethreonine protein kinase." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33859.

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In cell cycle checkpoint control, the Chk2 family protein kinases play a central role in mediating the cellular responses to DNA damage or DNA replication block. However, at the beginning of this project, there was no evidence for a Drosophila homologue of Chk2. loki was identified in a screen for serine/threonine protein kinases that are expressed in the ovary. Using a phylogenetic analysis, I showed that loki is a Drosophila chk2 orthologue. To characterize the checkpoint function of loki in Drosophila development, we created a loki null mutant and generated anti-Loki antibodies. Under norma

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