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Готові списки джерел за темами / Electrophoretic analysis / Статті в журналах

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Статті в журналах з теми "Electrophoretic analysis"

Автор: Grafiati

Опубліковано: 10 січня 2023

Оновлено: 28 січня 2023

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1

Folin, Marcella, and Eva Contiero. "Electrophoretic analysis of human hair keratins." Anthropologischer Anzeiger 53, no. 4 (December 5, 1995): 337–48. http://dx.doi.org/10.1127/anthranz/53/1995/337.

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2

Folin, Marcella, and Eva Contiero. "Electrophoretic analysis of mammalian hair keratins." Anthropologischer Anzeiger 54, no. 4 (December 12, 1996): 331–39. http://dx.doi.org/10.1127/anthranz/54/1996/331.

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3

Gabriel, Hancu, Tero-Vescan Amelia, Filip Cristina, and Rusu Aura. "Capillary Electrophoresis in the Analysis of Polyunsaturated Fatty Acids." Acta Medica Marisiensis 61, no. 4 (December 1, 2015): 378–81. http://dx.doi.org/10.1515/amma-2015-0103.

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AbstractThe aim of this study to inventory the main electrophoretic methods for identification and quantitative determination of fatty acids from different biological matrices. Critical analysis of electrophoretic methods reported in the literature show that the determination of polyunsaturated fatty acids can be made by: capillary zone electrophoresis, micellar electrokinetic chromatography and microemulsion electrokinetic chromatography using different detection systems such as ultraviolet diode array detection, laser induced fluorescence or mass – spectrometry. Capillary electrophoresis is a fast, low-cost technique used for polyunsaturated fatty acids analysis although their determination is mostly based on gas chromatography.

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4

Royer, John C., W. E. Hintz, R. W. Kerrigan, and P. A. Horgen. "Electrophoretic karyotype analysis of the button mushroom, Agaricus bisporus." Genome 35, no. 4 (August 1, 1992): 694–98. http://dx.doi.org/10.1139/g92-105.

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An efficient procedure is presented for the generation of protoplasts from shake flask cultures of the commercial mushroom Agaricus bisporus. Orthogonal field electrophoresis (OFAGE) of high molecular weight DNA from lysed protoplasts resolved the genome of A. bisporus into at least 10 bands, ranging in size from 1.2 to 4 Mb. The illustrated electrophoretic karyotypes of two homokaryons were highly polymorphic. A heterokaryon of the two homokaryons contained a mixture of the two electrophoretic patterns, though the ratio of nuclear types was not equal. A number of RFLP and RAPD markers and the rDNA repeat have been localized to specific chromosomes. Based on ethidium bromide staining intensity and autoradiography, we have estimated the chromosome number of A. bisporus to be 13.Key words: Agaricus bisporus, orthogonal field electrophoresis, RFLP, rDNA.

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5

Kelly, Robert H. "Electrophoretic analysis of proteins." Clinical Immunology Newsletter 13, no. 8 (August 1993): 93. http://dx.doi.org/10.1016/0197-1859(93)90015-c.

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6

Czupryn, Marta, and Kazimierz Toczko. "Variety-specificity of soluble proteins of potato tubers." Acta Societatis Botanicorum Poloniae 43, no. 4 (2015): 491–98. http://dx.doi.org/10.5586/asbp.1974.047.

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Separation of soluble tuber proteins from six potato clones and twelve varieties cultivated in Poland has been accomplished by disc electrophoresis. It was found that electrophoretic pattern was unique for a given clone or variety. Data obtained confirm results of the other authors for the other varieties and indicate that electrophoretic analysis of potato tuber proteins can be a useful method for taxonomic studies. Such analysis however cannot be used for genetic research since no correlations were found between electrophoretic patterns and genetic origin of respective clones and varieties.

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7

Wipf, Daniel, Jean-Philippe Bedell, Bernard Botton, Jean Charles Munch, and François Buscot. "Polymorphism in morels: isozyme electrophoretic analysis." Canadian Journal of Microbiology 42, no. 8 (August 1, 1996): 819–27. http://dx.doi.org/10.1139/m96-103.

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The aim of this study was to assess whether isozyme polymorphism in different members of the Morchellaceae could be used to improve the systematics in this fungal group and to characterize intraspecific crossings between monosporal strains in Morchella esculenta. For this purpose, isozyme electrophoretic analysis of the following enzymes was performed: glutamine synthetase, NAD–glutamate dehydrogenase, NADP–glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, NAD–glyceraldehyde phosphate dehydrogenase, glucose phosphate isomerase, and superoxide dismutase. The analyses allowed discrimination at the inter- or intra-specific levels and could help to establish a method of identification for strains in the Morchellaceae. To a certain extent they appeared to be suitable to analyze interactions of monosporal strains of Morchella esculenta in pairing experiments. The polymorphism shown in this study was consistent with the phylogenetic relationships between the investigated strains only at the genus level.Key words: isozyme analysis, electrophoresis, Morchella sp., polymorphism.

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8

Okuyama, Kazumi, Hiroshi Sugano, Fumitake Gejyo, and Masaaki Arakawa. "Electrophoretic analysis of tubular proteinuria. Immunological identification of electrophoretic components." SEIBUTSU BUTSURI KAGAKU 29, no. 6 (1985): 349–54. http://dx.doi.org/10.2198/sbk.29.349.

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9

Sato, Hirohisa, and Mariko Kudo. "Clinical diagnosis using electrophoretic analysis." Electrophoresis Letters 59, no. 1 (2015): 45–49. http://dx.doi.org/10.2198/electroph.59.45.

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10

Ferey, Ludivine, and Nathalie Delaunay. "Food Analysis on Electrophoretic Microchips." Separation & Purification Reviews 45, no. 3 (October 29, 2015): 193–226. http://dx.doi.org/10.1080/15422119.2015.1014049.

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11

Schulz, J. "Electrophoretic Methods in Protein Analysis." Journal of Electroanalytical Chemistry and Interfacial Electrochemistry 320, no. 3 (June 1991): 465. http://dx.doi.org/10.1016/0022-0728(91)85663-a.

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12

Schulz, J. "Electrophoretic Methods in Protein Analysis." Bioelectrochemistry and Bioenergetics 25, no. 3 (June 1991): 465. http://dx.doi.org/10.1016/0302-4598(91)80013-s.

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13

C.-Mendoza, C. E., T. Bhatti, and A. R. Bhatti. "Electrophoretic analysis of snake venoms." Journal of Chromatography B: Biomedical Sciences and Applications 580, no. 1-2 (September 1992): 355–63. http://dx.doi.org/10.1016/0378-4347(92)80543-y.

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14

Seitz, Ulrike, Peter J. Oefner, Suraphol Nathakarnkitkool, Michael Popp, and Günther K. Bonn. "Capillary electrophoretic analysis of flavonoids." Electrophoresis 13, no. 1 (1992): 35–38. http://dx.doi.org/10.1002/elps.1150130107.

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15

Wätzig, Hermann, and Gerhard Scriba. "Electrophoretic methods in pharmaceutical analysis." ELECTROPHORESIS 27, no. 12 (June 2006): 2261. http://dx.doi.org/10.1002/elps.200690040.

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16

Dunn, Michael J. "Electrophoretic Techniques for Protein Analysis." Journal of Chemical Technology & Biotechnology 51, no. 1 (April 24, 2007): 119–23. http://dx.doi.org/10.1002/jctb.280510114.

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17

Sikorski, Aleksander F. "Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins." Acta Societatis Botanicorum Poloniae 48, no. 4 (2015): 511–21. http://dx.doi.org/10.5586/asbp.1979.042.

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SDS-polyacrylamide electrophoretic patterns of <i>Chlamydomonas</i> flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides.

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18

Oto, Michiei, Akira Koguchi та Yasuhito Yuasa. "Analysis of a polyadenine tract of the transforming growth factor-β type II receptor gene in colorectal cancers by non-gel-sieving capillary electrophoresis". Clinical Chemistry 43, № 5 (1 травня 1997): 759–63. http://dx.doi.org/10.1093/clinchem/43.5.759.

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Abstract We developed a method to analyze a polyadenine tract, the (A)10 repeat, within the cysteine-rich domain of the transforming growth factor-β (TGF-β) type II receptor gene using a non-gel-sieving capillary electrophoresis technique and applied it to the DNA diagnosis of colorectal cancers. This method consists of single-strand DNA amplification of the (A)10 repeat by an asymmetric PCR technique and capillary electrophoresis. A higher concentration of dATP in the PCR reaction mixture led to more specific amplification of the (A)10 repeat. Under the optimal electrophoretic conditions, one nucleotide difference could be determined in 8 to 32 nucleotides. One or two base deletions of the (A)10 repeat in colorectal cancers could be detected under these conditions within 30 min, and the results coincided with those obtained on DNA sequencing analyses. According to a sensitivity study, we could detect the deleted sequence if it was present in 12.5% or more of the wild-type allele. The reproducibility of this technique was satisfactory because the intraassay imprecision (CV) (n = 10) was 1.4%. These results indicate that capillary electrophoretic analysis of small repeated sequences results in easier handling and more feasible automation, compared with conventional gel electrophoretic analysis.

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19

Jamasbi, Roudabeh J., Stephen J. Kennel, Larry C. Waters, Linda J. Foote, and J. Michael Ramsey. "Genetic Analysis ofPseudomonas aeruginosaby Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (PCR) and Arbitrarily Primed PCR: Gel Analysis Compared with Microchip Gel Electrophoresis." Infection Control & Hospital Epidemiology 25, no. 1 (January 2004): 65–71. http://dx.doi.org/10.1086/502295.

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AbstractObjectives:To assess the applicability of a newly emerging microchip gel electrophoresis for rapid strain differentiation among clinical isolates ofPseudomonas aeruginosa,and to compare this technique with the traditional gel method for DNA separation.Methods:One hundred clinical strains ofP. aeruginosaobtained from a hospital in northwestern Ohio were tested for reactivity to 3 serotype-specific monoclonal antibodies by enzyme-linked immunosorbent assay. Twelve strains (4 from each serogroup) were selected for DNA analysis by polymerase chain reaction (PCR)-based, single primer DNA fingerprinting methods with 3 different primers: 1 enterobacterial repetitive intergenic consensus PCR and 2 arbitrarily primed PCRs. The PCR products were analyzed by agarose slab gel and microchip gel electrophoresis.Results:Of the 100 clinical isolates tested, 39% (4%, 14%, and 21%) were found to be serotypes 0:3, 0:6, and 0:11, respectively. Twelve strains were chosen for DNA analysis by PCR. The PCR products were analyzed by agarose slab gel electrophoresis and on microchips to determine interspecies diversity. Both methods demonstrated that different serotypes exhibited different electrophoretic patterns. Two strains (clinical strains 6 and 7, serotype 0:6) showed identical patterns, indicating a high degree of relatedness.Conclusion:In all cases, there was concordance between the electrophoretic patterns detected by the two methods. The capability of conducting both PCR and microchip gel electrophoresis offers an opportunity for an automated and rapid method for genetic analysis and differentiation among strains ofP. aeruginosaand other microorganisms.

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20

Righetti, Pier Giorgio, Annalisa Castagna, Ben Herbert, and Giovanni Candiano. "How to Bring the “Unseen” Proteome to the Limelight via Electrophoretic Pre-Fractionation Techniques." Bioscience Reports 25, no. 1-2 (February 4, 2005): 3–17. http://dx.doi.org/10.1007/s10540-005-2844-2.

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The present review reports a panoply of electrophoretic methods as pre-fractionation tools in proteomic investigations in preparation for mass spectrometry or two-dimensional electrophoresis map analysis. Such electrophoretic pre-fractionation protocols include all those electrokinetic methodologies which are performed in free solution, most of them relying on isoelectric focusing steps (although some approaches based on gels and granulated media are also discussed). Devices associated with electrophoretic separations are multi-chamber apparatuses, such as the multi-compartment electrolyzers equipped with either isoelectric membranes or with isoelectric beads, Off-Gel electrophoresis in a multi-cup device and the Rotofor, an instrument also based on a multi-chamber system but exploiting the conventional technique of carrier-ampholyte-focusing. Other free-flow systems, as well as miniaturized chambers, are also described.

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21

Bücking, W., and T. Nann. "Electrophoretic analysis of gold nanoparticles: size-dependent electrophoretic mobility of nanoparticles." IEE Proceedings - Nanobiotechnology 153, no. 3 (2006): 47. http://dx.doi.org/10.1049/ip-nbt:20050043.

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22

Latzelsberger, M., and I. Krisai-Greilhuber. "Electrophoretic karyotype analysis in some species of Bolbitius and Conocybe (Bolbitiaceae, Agaricales)." Nova Hedwigia 70, no. 1-2 (February 1, 2000): 67–77. http://dx.doi.org/10.1127/nova.hedwigia/70/2000/67.

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23

Itoh, M., S. Sato, A. Moriyama, and M. Sasaki. "Effective pretreatment by cysteine proteinase inhibitor for improved analysis of protein components of trematodes on SDS-PAGE." Journal of Helminthology 64, no. 3 (September 1990): 175–79. http://dx.doi.org/10.1017/s0022149x00012128.

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ABSTRACTSince Fasciola sp. contained proteolytic enzyme(s), it was confirmed that degradation took place in protein components in extracts of the liver flukes, which resulted in lack of clarity of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Degradation was shown to occur mostly during a heating process of the extract samples. The proteolytic activity in the extracts was completely blocked and electrophoretic patterns were improved only by the use of cysteine proteinase inhibitor N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine (E-64). Great improvement was also noted in electrophoretic patterns of the extracts of other trematodes, such as Paragonimus westermani, P. miyazakii and Clonorchis sinensis, when their extracts were treated with E-64.

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24

Mamenko, T. P., L. V. Sirant, M. O. Dikun, and V. M. Pochinok. "Electrophoretic spectra and activity of peroxidase in winter plants of different varieties." Faktori eksperimental'noi evolucii organizmiv 22 (September 9, 2018): 138–43. http://dx.doi.org/10.7124/feeo.v22.938.

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Aim. To study electrophoretic spectra and activity of peroxidase in seedlings of wheat varieties, which differ in genetically determined characteristics. Methods. Biochemicals using a native gel-electrophoresis and a spectrophotometer. Results. Among the studied varieties, the highest peroxidase activity in seedlings varied winter wheat varieties of Astarta, spruce sparrows Polba Golikovskaya and high-amylose wheat HAW. When comparing the molecular forms of the enzyme, it was found that in the seedlings of the studied varieties there were 8–9 isoforms with peroxidase activity, which differed in relative electrophoretic mobility. Inactive isoforms and isoforms with relatively fast mobility were stable and recorded in all studied varieties. The revealed forms with average electrophoretic mobility were in 90 % of the studied varieties. Conclusions. Electrophoretic spectra of peroxidase in seedlings of the studied varieties differ significantly in number and mobility of its multiple molecular forms. Electrophoretic spectra and activity of peroxidase can be used as diagnostic features for comparative analysis of the studied plants in the early phases of ontogeny. Keywords: peroxidase, electrophoretic spectra, varieties, wheat (Triticum L.).

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25

Koutny, Lance B., and Edward S. Yeung. "Automated Image Analysis for Distortion Compensation in Sequencing Gel Electrophoresis." Applied Spectroscopy 46, no. 1 (January 1992): 136–41. http://dx.doi.org/10.1366/0003702924444461.

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A computerized method is described for correcting systematic distortion of images from slab gel electrophoresis. Such distortions can lead to misinterpretation of the information contained in the gel. The method is useful for data analysis in one-dimension slab gel electrophoresis where the information is manifested in rectangular shaped bands, such as conventional restriction digest or sequencing gels, and the distortions can be adequately described by continuous low-order polynomial functions. The purpose is to eliminate human skill and judgement from the process and to minimize human interaction, which would be useful in any future attempts to automate the analysis of electrophoretic gels.

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26

Sofer, William, and Presley Martin. "Analysis of densitometric data obtained from electrophoretic analysis." Bioinformatics 3, no. 2 (1987): 129. http://dx.doi.org/10.1093/bioinformatics/3.2.129.

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27

Gardner, Robert A. "An invariant-manifold analysis of electrophoretic traveling waves." Journal of Dynamics and Differential Equations 5, no. 4 (October 1993): 599–606. http://dx.doi.org/10.1007/bf01049140.

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28

Lilley, David M. J. "Analysis of branched nucleic acid structure using comparative gel electrophoresis." Quarterly Reviews of Biophysics 41, no. 1 (February 2008): 1–39. http://dx.doi.org/10.1017/s0033583508004678.

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AbstractElectrophoresis in polyacrylamide gels provides a simple yet powerful means of analyzing the relative disposition of helical arms in branched nucleic acids. The electrophoretic mobility of DNA or RNA with a central discontinuity is determined by the angle subtended between the arms radiating from the branchpoint. In a multi-helical branchpoint, comparative gel electrophoresis can provide a relative measure of all the inter-helical angles and thus the shape and symmetry of the molecule. Using the long–short arm approach, the electrophoretic mobility of all the species with two helical arms that are longer than all others is compared. This can be done as a function of conditions, allowing the analysis of ion-dependent folding of branched DNA and RNA species. Notable successes for the technique include the four-way (Holliday) junction in DNA and helical junctions in functionally significant RNA species such as ribozymes. Many of these structures have subsequently been proved correct by crystallography or other methods, up to 10 years later in the case of the Holliday junction. Just as important, the technique has not failed to date. Comparative gel electrophoresis can provide a window on both fast and slow conformational equilibria such as conformer exchange in four-way DNA junctions. But perhaps the biggest test of the approach has been to deduce the structures of complexes of four-way DNA junctions with proteins. Two recent crystallographic structures show that the global structures were correctly deduced by electrophoresis, proving the worth of the method even in these rather complex systems. Comparative gel electrophoresis is a robust method for the analysis of branched nucleic acids and their complexes.

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29

Mircia, Eleonora, Gabriel Hancu, Aura Rusu, Adriana Cazacu, Teodora Balaci, and Ruxandra Soare. "Determination of HMG-CoA reductase inhibitors by micellar electrokinetic chromatography." Acta Medica Marisiensis 62, no. 2 (June 1, 2016): 187–91. http://dx.doi.org/10.1515/amma-2016-0006.

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AbstractObjective: In this study we report the development of a simple, rapid and efficient capillary electrophoresis method for the simultaneous determination of atorvastatin, fluvastatin, lovastatin and simvastin.Methods: Capillary zone electrophoresis proved to be efficient for the simultaneous separation of atorvastatin and fluvastatin, but could not resolve the determination of lovastatin and simvastatin. The simultaneous separation of all four statins was achieved by applying a micellar electrokinetic chromatographic method, after transforming lovastatin and simvastatin in β-hydroxyl acid forms through alkaline hydrolysis. The optimum electrophoretic conditions and analytical parameters were investigated and the analytical performances of the method were verified with regard to linearity, precision, accuracy, LOD and LOQ.Results: The optimum electrophoretic separation conditions were: 25 mM sodium tetraborate with 25 mM sodium dodecyl sulphate buffer electrolyte at pH 9.5, applied voltage + 25 kV, separation temperature 25 °C, injection pressure/time 50 mbar/1 minutes, UV detection at 230 nm. Using the optimized electrophoretic conditions we succeeded in the simultaneous determination of the four statins in approximately 3 minutes, the order of migration being: atorvastatin, fluvastatin, lovastatin, simvastatin. The proposed method has been applied to the determination of the analytes in pharmaceutical tablets formulations.Conclusions: The capillary electrophoretic method developed in the present work proved to be suitable for the routine analysis of statins and can be adopted as quality control protocol in pharmaceutical analysis.

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30

Rogakou, E. P., C. Redon, C. Boon, K. Johnson, and W. M. Bonner. "Rapid Histone Extraction for Electrophoretic Analysis." BioTechniques 28, no. 1 (January 2000): 38–46. http://dx.doi.org/10.2144/00281bm06.

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31

Karousou, Evgenia, Manuela Viola, Davide Vigetti, Anna Genasetti, Manuela Rizzi, Moira Clerici, Barbara Bartolini, Giancarlo Luca, and Alberto Passi. "Analysis of Glycosaminoglycans by Electrophoretic Approach." Current Pharmaceutical Analysis 4, no. 2 (May 1, 2008): 78–89. http://dx.doi.org/10.2174/157341208784246260.

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32

Conradsen, Knut, and Jan Pedersen. "Analysis of Two-Dimensional Electrophoretic Gels." Biometrics 48, no. 4 (December 1992): 1273. http://dx.doi.org/10.2307/2532718.

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33

Kitamoto, Katsuhiko. "Electrophoretic Karyotype Analysis of Filamentous Fungi." Journal of the agricultural chemical society of Japan 68, no. 7 (1994): 1142–45. http://dx.doi.org/10.1271/nogeikagaku1924.68.1142.

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34

Lumb, R., JA Lanser, and PJ O'Donoghue. "Electrophoretic and immunoblot analysis ofCryptosporidium oocysts." Immunology and Cell Biology 66, no. 5-6 (October 1988): 369–76. http://dx.doi.org/10.1038/icb.1988.48.

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35

Deering, Norman R., and David Shalloway. "GelMetric: semi-automated electrophoretic mobility analysis." Bioinformatics 7, no. 1 (1991): 109–10. http://dx.doi.org/10.1093/bioinformatics/7.1.109.

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36

He, C., S. Fischer, G. A. Kullak-Ublick, N. Domingo, H. Lafont, and D. Jüngst. "Electrophoretic analysis of proteins in bile." Analytica Chimica Acta 383, no. 1-2 (March 1999): 185–203. http://dx.doi.org/10.1016/s0003-2670(98)00498-x.

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37

Grillon, F., D. Fayeulle, and M. Jeandin. "Quantitative image analysis of electrophoretic coatings." Journal of Materials Science Letters 11, no. 5 (1992): 272–75. http://dx.doi.org/10.1007/bf00729410.

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38

Lin, T. I., Y. H. Lee, and Y. C. Chen. "Capillary electrophoretic analysis of inorganic cations." Journal of Chromatography A 654, no. 1 (November 1993): 167–76. http://dx.doi.org/10.1016/0021-9673(93)83077-6.

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39

Marshall, Thomas, and Katherine M. Williams. "Electrophoretic analysis of Bence Jones proteinuria." Electrophoresis 20, no. 7 (June 1, 1999): 1307–24. http://dx.doi.org/10.1002/(sici)1522-2683(19990601)20:7<1307::aid-elps1307>3.0.co;2-p.

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40

Kan, J. A. L., A. Goverse, and C. J. B. Vlugt-Bergmans. "Electrophoretic karyotype analysis of Botrytis cinerea." Netherlands Journal of Plant Pathology 99, S3 (May 1993): 119–28. http://dx.doi.org/10.1007/bf03041402.

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41

Kajiwara, Hideyuki, and Andrew M. Hemmings. "Capillary electrophoretic analysis of ginseng polypeptide." Electrophoresis 19, no. 8-9 (June 1998): 1270–74. http://dx.doi.org/10.1002/elps.1150190808.

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42

Jinno, K., H. Sawada, and Y. Han. "Drug analysis by capillary electrophoretic methods." Biomedical Chromatography 12, no. 3 (May 1998): 126–27. http://dx.doi.org/10.1002/(sici)1099-0801(199805/06)12:3<126::aid-bmc777>3.0.co;2-h.

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43

Wessels, R. A., B. B. Rogers, C. N. Ou, R. Alcorn, and G. J. Buffone. "Liquid chromatography used in diagnosis of a rare hemoglobin combination: hemoglobin S/LeporeBoston." Clinical Chemistry 32, no. 5 (May 1, 1986): 903–6. http://dx.doi.org/10.1093/clinchem/32.5.903.

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Abstract "High-performance" liquid chromatography (HPLC), applied to hemoglobin analysis, is decidely more sensitive and gives better resolution than do routine electrophoretic methods. Here we present a case with a rare double heterozygote hemoglobin S/LeporeBoston, originally diagnosed as homozygous hemoglobin S by routine electrophoretic methods. Using a gradient elution weak cation-exchange HPLC technique, we could separate hemoglobin S and hemoglobin LeporeBoston and make the correct diagnosis. This case demonstrates how HPLC can be a useful adjunct to routine electrophoresis.

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44

Miranda, Silvia R. P., Silvana F. Fonseca, Maria S. Figueiredo, Myoko Yamamoto, Helena Z. W. Grotto, Sara T. O. Saad, and Fernando F. Costa. "Hb Köln [a2b298(FG5) val-met] identified by DNA analysis in a Brazilian family." Brazilian Journal of Genetics 20, no. 4 (December 1997): 745–48. http://dx.doi.org/10.1590/s0100-84551997000400030.

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Hb Köln was identified by DNA analysis in a Brazilian patient. A four-year old Brazilian female, with jaundice since birth, presented an abnormal band, between A2 and S, in hemoglobin electrophoresis on a cellulose acetate membrane, and a band with electrophoretic migration similar to Hb C on agar gel. Thermic instability and isopropanol precipitation tests were positive. Heinz bodies were observed in the patient’s peripheral blood. Sequencing of the three exons of the <FONT FACE="Symbol">b</font> globin gene detected a transition from G to A in the first position of codon 98. This alteration does not create or abolish any known restriction site. In this case, confirmation of the mutation was accomplished by allele-specific oligonucleotide hybridization, which is a simple and fast identification method when the clinical data and hematological and electrophoretic patterns are suggestive of Hb Köln.

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45

Zhang, Chenhua, Cong Bi, William Clarke, and David S. Hage. "Glycoform analysis of alpha1-acid glycoprotein based on capillary electrophoresis and electrophoretic injection." Journal of Chromatography A 1523 (November 2017): 114–22. http://dx.doi.org/10.1016/j.chroma.2017.08.032.

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46

Ferreira de Oliveira, Natacha, Ana Teresa Azevedo Sachetto, and Marcelo Larami Santoro. "Two-Dimensional Blue Native/SDS Polyacrylamide Gel Electrophoresis for Analysis of Brazilian Bothrops Snake Venoms." Toxins 14, no. 10 (September 23, 2022): 661. http://dx.doi.org/10.3390/toxins14100661.

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Viperidae snakes are the most important agents of snakebites in Brazil. The protein composition of snake venoms has been frequently analyzed by means of electrophoretic techniques, but the interaction of proteins in venoms has barely been addressed. An electrophoretic technique that has gained prominence to study this type of interaction is blue native polyacrylamide gel electrophoresis (BN-PAGE), which allows for the high-resolution separation of proteins in their native form. These protein complexes can be further discriminated by a second-dimension gel electrophoresis (SDS-PAGE) from lanes cut from BN-PAGE. Once there is no study on the use of bidimensional BN/SDS-PAGE with snake venoms, this study initially standardized the BN/SDS-PAGE technique in order to evaluate protein interactions in Bothrops atrox, Bothrops erythromelas, and Bothrops jararaca snake venoms. Results of BN/SDS-PAGE showed that native protein complexes were present, and that snake venom metalloproteinases and venom serine proteinases maintained their enzymatic activity after BN/SDS-PAGE. C-type lectin-like proteins were identified by Western blotting. Therefore, bidimensional BN/SDS-PAGE proved to be an easy, practical, and efficient method for separating functional venom proteins according to their assemblage in complexes, as well as to analyze their biological activities in further details.

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47

Spychalski, Przemysław, Dominik Poradowski, and Aleksander Chrószcz. "Histological and Electrophoretic Analysis of Carpathian barbel (Barbus carpathicus, Cyprinidae) Skin and Mucus in Environmental Context." Animals 10, no. 4 (April 8, 2020): 645. http://dx.doi.org/10.3390/ani10040645.

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Fish frequently serve as bioindicators of aquatic environments during their ecological evaluation. Carpathian barbel (Barbus carpathicus, Cyprinidae) is a species common to rivers and lakes of Eurasia and Africa. Seasons of the year can influence its skin morphology and mucus composition. The clinical status of the animal depends on the above-mentioned factors. The aim of this study was a histological, histometrical and electrophoretical analysis of periodical changes in barbel common integument. The accessible material was investigated in histological, cytological and electrophoretic analysis using hematoxylin-eosin staining, histometric morphometry, gel electrophoresis and cytological methods. The results demonstrated significant differences in the investigated parameters for spring–summer and autumn–winter periods. Both skin epithelium morphology (epithelium thickness, number of cell layers, melanophores and mucous cell existence) and mucus composition (proteins, immune system cells, keratinocytes and mucocytes) showed significant differences between investigated seasons. These morphological and physiological changes were more pronounced in the dorsal than ventral regions of common integument. The differences in the physical characteristics of mucus and the histological structure of the skin cannot only serve as a source of useful information about an evaluated ecosystem, but can be also related to additional factors, e.g., microbiological and chemical water contamination.

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48

Grenier, Jean, François Côté, and Alain Asselin. "Analysis of extracellular proteins by native polyacrylamide gel electrophoresis with infiltrated leaf tissue: application to pathogenesis-related proteins." Canadian Journal of Botany 66, no. 6 (June 1, 1988): 1227–29. http://dx.doi.org/10.1139/b88-174.

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In addition to polyacrylamide gel electrophoretic analysis of intercellular fluid extracts, a simple method of detection of extracellular pathogenesis-related proteins was based on direct native polyacrylamide gel electrophoresis for acidic and basic proteins with leaf tissue infiltrated with 150 mM sucrose. This technique allowed for the detection of the complete set of tobacco pathogenesis-related proteins without having to extract the intercellular fluid by low-speed centrifugation. A major advantage of the technique is the capacity to observe the distribution of extracellular endogenous or exogenous proteins in the tissue directly subjected to electrophoresis.

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49

Hodgkiss, M. T., and J. W. Moodie. "Genomic Analysis of RNA Viruses Isolated from Water." Water Science and Technology 17, no. 10 (October 1, 1985): 15–21. http://dx.doi.org/10.2166/wst.1985.0091.

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Polyacrylamide gel electrophoresis (PAGE) was used to distinguish human from animal rotaviruses and type four reoviruses isolated from sewage effluent. A simple method of extracting the ds RNA was used without resorting to viral purification. The electrophoretic patterns were clearly discernible after silver nitrate staining. The human rotaviruses were of different strains and could be easily distinguished from the simian agent. All four reoviruses were of the same type, Type 3, but appeared to be of a different strain from the control type 3 with which they were compared. The feasibility of building a collection of various animal and human rota- and reovirus genomic profiles is discussed.

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50

Steinberger, Stephanie, Sobha Karuthedom George, Lucia Lauková, René Weiss, Carla Tripisciano, Ruth Birner-Gruenberger, Viktoria Weber, Günter Allmaier, and Victor U. Weiss. "A possible role of gas-phase electrophoretic mobility molecular analysis (nES GEMMA) in extracellular vesicle research." Analytical and Bioanalytical Chemistry 413, no. 30 (October 7, 2021): 7341–52. http://dx.doi.org/10.1007/s00216-021-03692-y.

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AbstractThe emerging role of extracellular vesicles (EVs) as biomarkers and their envisioned therapeutic use require advanced techniques for their detailed characterization. In this context, we investigated gas-phase electrophoresis on a nano electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA, aka nES differential mobility analyzer, nES DMA) as an alternative to standard analytical techniques. In gas-phase electrophoresis, single-charged, surface-dry, native, polydisperse, and aerosolized analytes, e.g., proteins or bio-nanoparticles, are separated according to their electrophoretic mobility diameter, i.e., globular size. Subsequently, monodisperse particles are counted after a nucleation step in a supersaturated atmosphere as they pass a focused laser beam. Hence, particle number concentrations are obtained in accordance with recommendations of the European Commission for nanoparticle characterization (2011/696/EU from October 18th, 2011). Smaller sample constituents (e.g., co-purified proteins) can be detected next to larger ones (e.g., vesicles). Focusing on platelet-derived EVs, we compared different vesicle isolation techniques. In all cases, nanoparticle tracking analysis (NTA) confirmed the presence of vesicles. However, nES GEMMA often revealed a significant co-purification of proteins from the sample matrix, precluding gas-phase electrophoresis of less-diluted samples containing higher vesicle concentrations. Therefore, mainly peaks in the protein size range were detected. Mass spectrometry revealed that these main contaminants belonged to the group of globulins and coagulation-related components. An additional size exclusion chromatography (SEC) step enabled the depletion of co-purified, proteinaceous matrix components, while a label-free quantitative proteomics approach revealed no significant differences in the detected EV core proteome. Hence, the future in-depth analysis of EVs via gas-phase electrophoresis appears feasible. Graphical abstract

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