Добірка наукової літератури з теми "Endocytosis"

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Статті в журналах з теми "Endocytosis"

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Bouchard, Beth A., Joseph M. Petty, and Benjamin T. Suratt. "Endocytosis of Factor V by Ex Vivo-Derived Mouse Megakaryocytes is Dependent Upon Low Density Lipoprotein Receptor-Related Protein-1." Blood 114, no. 22 (2009): 4014. http://dx.doi.org/10.1182/blood.v114.22.4014.4014.

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Abstract Abstract 4014 Poster Board III-950 Subsequent to platelet activation at sites of vascular injury, numerous hemostatic proteins are released from platelet α-granules. As platelets possess little, if any, biosynthetic capability, these proteins are either synthesized by megakaryocytes (e.g. vonWillebrand) or endocytosed from the plasma by both megakaryocytes and platelets (e.g. fibrinogen). In contrast, in humans, factor V is endocytosed exclusively by megakaryocytes from the plasma via a specific, receptor-mediated, clathrin-dependent mechanism, requiring two membrane proteins. Factor
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Fares, Hanna, and Iva Greenwald. "Genetic Analysis of Endocytosis in Caenorhabditis elegans: Coelomocyte Uptake Defective Mutants." Genetics 159, no. 1 (2001): 133–45. http://dx.doi.org/10.1093/genetics/159.1.133.

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Abstract The coelomocytes of Caenorhabditis elegans are scavenger cells that continuously and nonspecifically endocytose fluid from the pseudocoelom (body cavity). Green fluorescent protein (GFP) secreted into the pseudocoelom from body wall muscle cells is endocytosed and degraded by coelomocytes. We show that toxin-mediated ablation of coelomocytes results in viable animals that fail to endocytose pseudocoelomic GFP, indicating that endocytosis by coelomocytes is not essential for growth or survival of C. elegans under normal laboratory conditions. We examined known viable endocytosis mutant
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Bouchard, Beth A., Douglas J. Taatjes, Natalie T. Meisler та Paula B. Tracy. "Subsequent to Its Endocytosis by Megakaryocytes, Factor V Is Trafficked to the [Italic]cis[/Italic]-Golgi Network Prior to Its Storage in α-Granules." Blood 108, № 11 (2006): 1697. http://dx.doi.org/10.1182/blood.v108.11.1697.1697.

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Abstract Human platelet-derived factor V originates from megakaryocyte endocytosis of its plasma counterpart. Circulating platelets do not endocytose plasma-derived factor V and little, if any, of this hemostatically relevant, and unique, cofactor protein is synthesized by megakaryocytes. Fibrinogen, another α-granule protein, is also endocytosed from plasma, whereas other similarly stored proteins are synthesized by megakaryocytes, e.g. vWF and P-selectin. Using CD34+ex vivo-derived megakaryocytes as a model, mechanisms defining megakaryocyte endocytosis of plasma-derived factor V and its int
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Roweth, Harvey G., Michael Malloy, Jodi A. Forward, et al. "The Effects of Antiplatelet Agents on Endocytosis." Blood 134, Supplement_1 (2019): 1058. http://dx.doi.org/10.1182/blood-2019-131912.

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Almost every platelet-derived protein originates from either megakaryocytes (MKs), or the endocytosis of factors within blood circulation. These endocytosed factors can be locally released upon platelet activation to regulate hemostasis, or to promote the growth and neovascularization of solid tumors. Although platelet endocytosis has long been recognized, our mechanistic understanding remains largely confined to receptor-mediated endocytosis of fibrinogen via integrin αIIbβ3. Whether antiplatelet therapy and/or different disease states influence platelet endocytosis remain understudied areas
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Rivera, J., J. M. Mullins, K. Furuichi, and C. Isersky. "Endocytosis of aggregated immunoglobulin G by rat basophilic leukemia cells; rate, extent, and effects on the endocytosis of immunoglobulin E." Journal of Immunology 136, no. 2 (1986): 623–27. http://dx.doi.org/10.4049/jimmunol.136.2.623.

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Abstract Rat basophilic leukemia (RBL) cells have distinct receptors for IgE and IgG. We assessed the endocytosis of chemically and immunochemically cross-linked mouse-IgG and its influence on the simultaneous endocytosis of IgE. We found that at 37 degrees C, aggregates of IgG and IgE were endocytosed at about the same rate with one-half of the maximal endocytosis occurring in 5 to 13 min, and the efficiency of endocytosis for both ligands ranging from 40 to 70%. We also found that endocytosis of cross-linked IgE and IgG occurred simultaneously and neither ligand significantly affected the ra
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Vocelle, Daniel, Olivia M. Chesniak, Amanda P. Malefyt, et al. "Dextran functionalization enhances nanoparticle-mediated siRNA delivery and silencing." TECHNOLOGY 04, no. 01 (2016): 42–54. http://dx.doi.org/10.1142/s2339547816400100.

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Understanding the endocytosis and intracellular trafficking of short interfering RNA (siRNA) delivery vehicle complexes remains a critical bottleneck in designing siRNA delivery vehicles for highly active RNA interference (RNAi)-based therapeutics. In this study, we show that dextran functionalization of silica nanoparticles enhanced uptake and intracellular delivery of siRNAs in cultured cells. Using pharmacological inhibitors for endocytotic pathways, we determined that our complexes are endocytosed via a previously unreported mechanism for siRNA delivery in which dextran initiates scavenger
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Eyre, Jeanette, Kyriakos Ioannou, Blair D. Grubb, et al. "Statin-sensitive endocytosis of albumin by glomerular podocytes." American Journal of Physiology-Renal Physiology 292, no. 2 (2007): F674—F681. http://dx.doi.org/10.1152/ajprenal.00272.2006.

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Glomerular podocytes are critical regulators of glomerular permeability via the slit diaphragm and may play a role in cleaning the glomerular filter. Whether podocytes are able to endocytose proteins is uncertain. We studied protein endocytosis in conditionally immortalized mouse and human podocytes using FITC-albumin by direct quantitative assay and by fluorescence microscopy and electron microscopy in mouse podocytes. Furthermore, in vivo uptake was studied in human, rat, and mouse podocytes. Both mouse and human podocytes displayed specific one-site binding for FITC-albumin with Kd of 0.91
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Bouchard, Beth A., Natalie T. Meisler, Michael E. Nesheim, and Paula B. Tracy. "Uptake of Factor V by Megakaryocytes Requires a Specific Factor V Receptor Linked to a Low-Density Lipoprotein Receptor-Related Protein." Blood 106, no. 11 (2005): 688. http://dx.doi.org/10.1182/blood.v106.11.688.688.

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Abstract Factor V is endocytosed by megakaryocytes from plasma to form the platelet-derived factor V/Va pool via a receptor-mediated, clathrin-dependent mechanism. However, the megakaryocyte receptor that mediates the binding and subsequent endocytosis of plasma-derived factor V is unknown. Because of its known ability to interact with proteins involved in coagulation and fibrinolysis, the role of low-density lipoprotein receptor-related protein (LRP) or an LRP-like molecule was examined in factor V endocytosis by the megakaryocyte-like cell line CMK. As endocytosis by such proteins is Ca2+-de
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Ivesic, Caroline, Stefanie Krammer, Marianne Koller-Peroutka, et al. "Quantification of Protein Uptake by Endocytosis in Carnivorous Nepenthales." Plants 12, no. 2 (2023): 341. http://dx.doi.org/10.3390/plants12020341.

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Carnivorous plants adsorb prey-derived nutrients partly by endocytosis. This study quantifies endocytosis in Drosophyllum lusitanicum, Drosera capensis, Drosera roseana, Dionaea muscipula and Nepenthes × ventrata. Traps were exposed to 1% fluorescent-labeled albumin (FITC-BSA), and uptake was quantified repeatedly for 64 h. Formation of vesicles started after ≤1 h in adhesive traps, but only after 16 h in species with temporary stomach (D. muscipula and N. × ventrata). In general, there are similarities in the observed species, especially in the beginning stages of endocytosis. Nonetheless, fu
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Parks, A. L., K. M. Klueg, J. R. Stout, and M. A. Muskavitch. "Ligand endocytosis drives receptor dissociation and activation in the Notch pathway." Development 127, no. 7 (2000): 1373–85. http://dx.doi.org/10.1242/dev.127.7.1373.

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Endocytosis of the ligand delta; is required for activation of the receptor Notch during Drosophila development. The Notch extracellular domain (NotchECD) dissociates from the Notch intracellular domain (NotchICD) and is trans-endocytosed into delta;-expressing cells in wild-type imaginal discs. Reduction of dynamin-mediated endocytosis in developing eye and wing imaginal discs reduces Notch dissociation and Notch signalling. Furthermore, dynamin-mediated delta endocytosis is required for Notch trans-endocytosis in Drosophila cultured cell lines. Endocytosis-defective delta proteins fail to me
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Дисертації з теми "Endocytosis"

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Haglund, Kaisa. "Ubiquitination and Receptor Endocytosis." Doctoral thesis, Uppsala University, Ludwig Institute for Cancer Research, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4259.

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<p>Protein ubiquitination is an evolutionary conserved mechanism that controls a wide variety of cellular functions. Polyubiquitinated proteins are generally degraded in the proteasome, whereas monoubiquitination controls various other cellular processes, including endocytosis and endosomal sorting.</p><p>Termination of signaling by activated receptor tyrosine kinases (RTKs) largely occurs via their endocytosis and subsequent lysosomal degradation, processes accompanied by receptor ubiquitination. Cbl family proteins are major ubiquitin ligases that promote RTK ubiquitination and downregulatio
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Webster, Paul. "Endocytosis by African trypanosomes." Thesis, Brunel University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280722.

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Kalkman, Edward R. I. C. "Endocytosis in filamentous fungi." Thesis, University of Edinburgh, 2007. http://hdl.handle.net/1842/1970.

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Endocytosis is little understood in filamentous fungi. For some time it has been controversial as to whether endocytosis occurs in filamentous fungi. A comparative genomics analysis between Saccharomyces cerevisiae and 10 genomes of filamentous fungal species showed that filamentous fungi possess complex endocytic machineries. The use of the endocytic marker dye FM4-64, and various vesicle trafficking inhibitors revealed many similarities between endocytosis in the filamentous fungus Neurospora crassa, and endocytosis in budding yeast and mammalian cells. Actin polymerization was found to be c
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Gagliardi, M. "Endocytosis and wingless signalling." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1353109/.

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Wingless (Drosophila Wnt-1) is secreted glycoprotein that triggers an evolutionary conserved signal transduction pathway. The role of endocytosis in Wnt/Wingless signaling is not clearly understood and highly debated. In my thesis I explore the role that endocytosis/endocytic trafficking has on Wingless signalling activation and termination. In the canonical pathway Wingless binds to a member of the Frizzled family of seven-pass transmembrane receptor (Frizzled1 or Frizzled2) and to Arrow. Formation of this trimeric complex leads to the inactivation of the Armadillo degradation complex and tra
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Rehman, Michael. "Analysis of endocytosis at eisosomes." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-135951.

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Jha, Ankita. "Quantitative control of GPCR organization and signaling by endocytosis in epithelial morphogenesis." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0393/document.

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Au cours de la gastrulation de l’embryon de Drosophile, l’activation apicale du cytosquelette d’acto-myosine orchestre la constriction apicale dans le mésoderme en invagination ainsi que l’intercalation cellulaire dans l’ectoderme en extension. Un contrôle quantitatif de l’activité des GPCRs et, par conséquent, de l’activation de Rho1 est à l’origine des différences de déformation des cellules du mésoderme et de l’ectoderme mais ces mécanismes demeurent incompris. L’activité du GPCR Smog se concentre respectivement en deux compartiments distincts à la surface de la membrane plasmique (PM) et d
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Shurety, Wenda. "Apical endocytosis in Caco-2 cells." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242912.

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Karagiannis, Sophia. "The process of endocytosis of CD23." Thesis, King's College London (University of London), 1995. https://kclpure.kcl.ac.uk/portal/en/theses/the-process-of-endocytosis-of-cd23(553202e7-a9c8-444e-b0a5-f7561d1e6297).html.

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Nichols, II James Tucker. "DSL-ligand endocytosis and notch signaling." Diss., Restricted to subscribing institutions, 2008. http://proquest.umi.com/pqdweb?did=1692099791&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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Wilbur, Jeremy D. "Conformational switches regulate clathrin mediated endocytosis." Diss., Search in ProQuest Dissertations & Theses. UC Only, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3324583.

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Книги з теми "Endocytosis"

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Pastan, Ira, and Mark C. Willingham, eds. Endocytosis. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4615-6904-6.

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Courtoy, Pierre J., ed. Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5.

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H, Pastan Ira, and Willingham Mark C, eds. Endocytosis. Plenum Press, 1985.

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Mark, Marsh, ed. Endocytosis. Oxford University Press, 2001.

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Šamaj, Jozef, František Baluška, and Diedrik Menzel, eds. Plant Endocytosis. Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/b103851.

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Šamaj, Jozef, ed. Endocytosis in Plants. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-32463-5.

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Lamaze, Christophe, and Ian Prior, eds. Endocytosis and Signaling. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96704-2.

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Swan, Laura E., ed. Clathrin-Mediated Endocytosis. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8719-1.

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Ivanov, Andrei I., ed. Exocytosis and Endocytosis. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-178-9.

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Ivanov, Andrei I., ed. Exocytosis and Endocytosis. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0944-5.

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Частини книг з теми "Endocytosis"

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De Brabander, M., R. Nuydens, and H. Geerts. "Dynamic Cytomatrix-Membrane Interactions Investigated with Nanovid Microscopy." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_1.

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Storrie, B., and Y. Deng. "Protein Exchange Within the Lysosome and Pre-Lysosome Compartment: A Mechanism for Maintaining Organelle Functionality?" In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_10.

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Murphy, Robert F., Mario Roederer, David M. Sipe, Cynthia Corley Cain, and Russell B. Wilson. "Endosomal pH Regulation and the Maturation Model for Lysosome Biogenesis." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_11.

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Pypaert, Marc, and Graham Warren. "Effect of ATPγS on the Formation of Coated Vesicles in Broken Hela Cells." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_12.

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Schmid, Sandra L., Laura L. Carter, and Elizabeth Smythe. "ATP is Required for Receptor-Mediated Endocytosis Both in Vivo and in Vitro." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_13.

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Gruenberg, Jean, Jean-Pierre Gorvel, and Morgane Bomsel. "Regulation of Endocytic Membrane Traffic." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_14.

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Luzio, J. Paul, and Barbara M. Mullock. "The Interaction of Late Endosomes with Lysosomes in a Cell-Free System." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_15.

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Howell, Kathryn. "Roundtable on Cell-Free Systems." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_16.

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Fuchs, R., S. Schmid, I. Mellman, and H. Klapper. "Regulation of ATP-Dependent Endosome Acidification." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_17.

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Desbuquois, Bernard, François Authier, Jean-Pierre Clot, Michel Janicot, and Françoise Fouque. "Degradation of Insulin and Glucagon in Isolated Liver Endosomes: Functional Relationships with ATP-Dependent Endosomal Acidification and Partial Characterization of Degradation Products." In Endocytosis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84295-5_18.

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Тези доповідей конференцій з теми "Endocytosis"

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Nieto-Chaupis, Huber. "Endocytosis of Charged Electrically Nano Drugs Described by Boltzmann Equation." In 2024 IEEE 22nd Student Conference on Research and Development (SCOReD). IEEE, 2024. https://doi.org/10.1109/scored64708.2024.10872723.

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Guan, Yingxue, Aili Zhang, and Lisa X. Xu. "Theoretical Study of Cellular Uptake of QD Nanoparticles." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53361.

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Cellular uptake kinetics of nanoparticles is one of the key issues determining the design and application of the particles. It is widely accepted that the nanoparticles getting into the cells mainly by endocytosis[1], a process which is found to be inhibited distinctly when temperature is below 4 °C. In fact, some nanoparticles could enter cells at lower temperatures or when the endocytosis was blocked[2–4]. Models describing the intrusion of the nanoparticles into cells only take the endocytosis process into consideration5. Meanwhile, other than the factors as concentration, size, electrical
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Proskura, A. L., S. O. Vechkapova, and E. D. Sorokoumov. "MECHANISMS OF AMPA RECEPTOR ENDOCYTOSIS IN THE PROCESSES OF SYNAPTIC PLASTICITY OF THE HIPPOCAMPUS." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-267.

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Endocytosis of AMPA type glutamate receptors is a key event in the regulation of synaptic plasticity in the hippocampus. The work presents a reconstruction of the connection of synaptic signaling pathways with the molecular mechanisms of AMPA receptor endocytosis. GeneNet technology (ROSPATENT No. 990006 dated 15/02/1999) was used to reconstruct protein-protein interactions based on open sources of the PubMed database.
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Ciobanu, Gabriel. "On the power of endocytosis and exocytosis." In 2008 3rd International Conference on Bio-Inspired Computing: Theories and Applications (BIC-TA 2008). IEEE, 2008. http://dx.doi.org/10.1109/bicta.2008.4656694.

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Nuzzo, G. "Fractional diffusion of membrane receptors in endocytosis pathway." In AIMETA 2022. Materials Research Forum LLC, 2023. http://dx.doi.org/10.21741/9781644902431-50.

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Abstract. In this paper the diffusion model representing the motion of membrane receptors with respect to virus endocytosis is considered in the context of applied mechanics. The unexpected behaviour of the receptor density that moves from higher concentrations in the unbound phase to lower concentration at the right of the virus surface is accounted for introducing a mechanical drift term in the governing equation so that the difference of concentrations, higher in the bounded phase and lower in the unbounded phase is accounted for in the receptor motion. Additionally, a non-gaussian model of
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Massaeli, Hamid, Divya Viswanathan, Dhanya Pillai, and Nasrin Mesaeli. "Regulation Of Caveolin-dependent Endocytosis By Endoplasmic Reticulum Chaperones." In Qatar Foundation Annual Research Conference Proceedings. Hamad bin Khalifa University Press (HBKU Press), 2014. http://dx.doi.org/10.5339/qfarc.2014.hbpp1034.

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Grøvdal, Lene M., Lasse Henriksen, Michael V. Grandal, Stine LJ Knudsen, and Bo van Deurs. "Abstract 264: EGFR endocytosis after binding of different ligands." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-264.

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Kyung- Hwa Yoo. "Capacitance-based real time monitoring of receptor-mediated endocytosis." In 2010 Conference on Precision Electromagnetic Measurements (CPEM 2010). IEEE, 2010. http://dx.doi.org/10.1109/cpem.2010.5544238.

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Dufour, A., V. Meas-Yedid, A. Grassart, and J. C. Olivo-Marin. "Automated quantification of cell endocytosis using active contours and wavelets." In 2008 19th International Conference on Pattern Recognition (ICPR). IEEE, 2008. http://dx.doi.org/10.1109/icpr.2008.4761748.

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10

Khan, Imran, and Patricia S. Steeg. "Abstract 1047: Role of endocytosis in NM23 mediated motility suppression." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-1047.

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Звіти організацій з теми "Endocytosis"

1

Ceresa, Brian, and Sandra L. Schmid. EGF-Receptor Signaling in Endocytosis Deficient Cells. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada396679.

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2

Ceresa, Brian, and Sandra Schmid. EGF-Receptor Signaling in Endocytosis Deficient Cells. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada383645.

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3

Schmid, Sandra L., and Christophe Lamaze. Characterization of Ligand-Induced Endocytosis of EGF Receptors. Defense Technical Information Center, 1995. http://dx.doi.org/10.21236/ada300531.

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4

Schmid, Sandra L., and Christophe LAmaze. Characterization of Ligand-Induced Endocytosis of EGF - Receptors. Defense Technical Information Center, 1996. http://dx.doi.org/10.21236/ada314743.

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5

Lee, Anthony, and Mark A. Lemmon. Study of the Regulation of ErbB Signaling by Receptor-Mediated Endocytosis. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada406114.

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6

Lee, Anthony. Study of the Regulation of erbB Signaling by Receptor-Mediated Endocytosis. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada406140.

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7

Lee, Anthony, and Mark A. Lemmon. Study of the Regulation of erbB Signaling by Receptor-mediated Endocytosis. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada383058.

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8

El Bejjani, Rachid M. Effect of MUC1 Expression on EGFR Endocytosis and Degradation in Human Breast Cancer Cell Lines. Defense Technical Information Center, 2009. http://dx.doi.org/10.21236/ada504024.

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9

El Bejjani, Rachid M. Effect of MUC1 Expression on EGFR Endocytosis and Degradation in Human Breast Cancer Cell Lines. Defense Technical Information Center, 2007. http://dx.doi.org/10.21236/ada470580.

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10

Brittingham, Katherine C., Gordon Ruthel, Rekha G. Panchal, Claudette L. Fuller, and Wilson J. Ribot. Dendritic Cells Endocytose Bacillus Anthracis Spores: Implications for Anthrax Pathogenesis. Defense Technical Information Center, 2005. http://dx.doi.org/10.21236/ada434591.

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