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Готові списки джерел за темами / Enzyme activation

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Добірка наукової літератури з теми "Enzyme activation"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 25 липня 2025

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Статті в журналах з теми "Enzyme activation"

1

Wollenberger, Ulla, and Frieder W. Scheller. "Enzyme activation for activator and enzyme activity measurement☆." Biosensors and Bioelectronics 8, no. 6 (1993): 291–97. http://dx.doi.org/10.1016/0956-5663(93)85009-d.

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2

Wang, Fang, Yuchen Liu, Chang Du, and Renjun Gao. "Current Strategies for Real-Time Enzyme Activation." Biomolecules 12, no. 5 (2022): 599. http://dx.doi.org/10.3390/biom12050599.

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Enzyme activation is a powerful means of achieving biotransformation function, aiming to intensify the reaction processes with a higher yield of product in a short time, and can be exploited for diverse applications. However, conventional activation strategies such as genetic engineering and chemical modification are generally irreversible for enzyme activity, and they also have many limitations, including complex processes and unpredictable results. Recently, near-infrared (NIR), alternating magnetic field (AMF), microwave and ultrasound irradiation, as real-time and precise activation strate

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3

Hamilton-Miller, J. M. T., and Q. Li. "Enzyme-Catalyzed Antimicrobial Activation." Antimicrobial Agents and Chemotherapy 46, no. 11 (2002): 3692. http://dx.doi.org/10.1128/aac.46.11.3692.2002.

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4

Hadfield, Andrea T. "Electron-Induced Enzyme Activation." Structure 14, no. 1 (2006): 1–2. http://dx.doi.org/10.1016/j.str.2005.12.002.

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5

Bott, R., G. Ganshaw, M. Soltis, P. Kuhn, and M. Knapp. "Snapshots of Enzyme Activation." Acta Crystallographica Section A Foundations of Crystallography 56, s1 (2000): s247. http://dx.doi.org/10.1107/s0108767300025319.

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6

Cassels, R., R. Fears, and R. A. Smith. "The interaction of streptokinase.plasminogen activator complex, tissue-type plasminogen activator, urokinase and their acylated derivatives with fibrin and cyanogen bromide digest of fibrinogen. Relationship to fibrinolytic potency in vitro." Biochemical Journal 247, no. 2 (1987): 395–400. http://dx.doi.org/10.1042/bj2470395.

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The effects of purified soluble fibrin and of fibrinogen fragments (fibrin mimic) on the activation of Lys-plasminogen (i.e. plasminogen residues 77-790) to plasmin by streptokinase.plasminogen activator complex and by tissue-type plasminogen activator were studied. Dissociation constants of both activators were estimated to lie in the range 90-160 nM (fibrin) and 16-60 nM (CNBr-cleavage fragments of fibrinogen). The kinetic mechanism for both types of activator comprised non-essential enzyme activation via a Rapid Equilibrium Ordered Bireactant sequence. In order to relate the fibrin affinity

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7

Arcus, Vickery L., and Adrian J. Mulholland. "Temperature, Dynamics, and Enzyme-Catalyzed Reaction Rates." Annual Review of Biophysics 49, no. 1 (2020): 163–80. http://dx.doi.org/10.1146/annurev-biophys-121219-081520.

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We review the adaptations of enzyme activity to different temperatures. Psychrophilic (cold-adapted) enzymes show significantly different activation parameters (lower activation enthalpies and entropies) from their mesophilic counterparts. Furthermore, there is increasing evidence that the temperature dependence of many enzyme-catalyzed reactions is more complex than is widely believed. Many enzymes show curvature in plots of activity versus temperature that is not accounted for by denaturation or unfolding. This is explained by macromolecular rate theory: A negative activation heat capacity f

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8

KHOLODENKO, Boris N., and Guy C. BROWN. "Paradoxical control properties of enzymes within pathways: can activation cause an enzyme to have increased control?" Biochemical Journal 314, no. 3 (1996): 753–60. http://dx.doi.org/10.1042/bj3140753.

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It is widely assumed that within a metabolic pathway inhibition of an enzyme causes the control exerted by that enzyme over the flux through its own reaction to increase, whereas activation causes its control to decrease. This assumption forms the basis of a number of experimental methods. For a pathway conceptually divided into two enzyme groups connected via a single metabolite we have derived a general condition under which this assumption is false, and thus the pathway shows paradoxical control behaviour, i.e. increased control with activation and decreased control with inhibition of an en

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9

Vater, C. A., H. Nagase, and E. D. Harris. "Proactivator-dependent activation of procollagenase induced by treatment with EGTA." Biochemical Journal 237, no. 3 (1986): 853–58. http://dx.doi.org/10.1042/bj2370853.

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A new mechanism for activation of the proactivator of procollagenase [Vater, Nagase & Harris (1983) J. Biol. Chem. 258, 9374-9382] has been found. Collagenolytic and other proteolytic enzyme activities in the medium of cultured rabbit synovial fibroblasts were found to be activated by a new mechanism: short-term incubation at 37 degrees C performed in the presence of EGTA followed by replacement of Ca2+ during enzyme assay. The crucial event in procollagenase activation is the production of a functional activator enzyme. Activation of procollagenase in the culture medium did not occur when

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10

Shisler, Krista A., Rachel U. Hutcheson, Masaki Horitani, et al. "Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme." Journal of the American Chemical Society 139, no. 34 (2017): 11803–13. http://dx.doi.org/10.1021/jacs.7b04883.

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Дисертації з теми "Enzyme activation"

1

Eddeb, Fadel. "Stabilisation and activation of horseradish peroxidase." Thesis, University of Newcastle Upon Tyne, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294853.

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2

Aubatin, Aude. "Modulation de la réponse immune par IL4I1 : rôle dans les évènements précoces d’activation lymphocytaire T." Thesis, Paris Est, 2016. http://www.theses.fr/2016PESC0069.

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Modulation de la réponse lymphocytaire T par IL4I1 - RESUME : L’enzyme Interleukin-four Induced Gene 1 (IL4I1), qui dégrade la phénylalanine, est exprimée par des cellules présentatrices d’antigène (CPA) en réponse à des stimuli pro-inflammatoires. Elle affecte la prolifération et les fonctions lymphocytaires T et pourrait donc participer au rétrocontrôle des réponses immunitaires. Cependant, les mécanismes d’action d’IL4I1 restent encore mal connus. Mon projet de thèse a comporté deux axes. Dans un premier axe, j’ai participé à la caractérisation du rôle d’IL4I1 dans la différenciation des ly

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3

Quayle, Katherine Amanda. "Mechanisms of regulation of acetyl-CoA carboxylase." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30657.

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One of the major physiological responses to insulin secretion is the activation of lipogenesis in target tissues (principally fat and liver). As acetyl-CoA carboxylase (ACC) is the rate limiting enzyme in fatty acid synthesis, the mechanisms involved in the short term regulation of this enzyme represent a pertinent model system for determining elements involved in amplification of the signals produced in response to stimulation of cells with lipogenic and counter regulatory hormones. The regulation of mammalian ACC by hormones is a complex phenomenon involving interplay between allosteric and

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4

Lee, Charles Kai-Wu. "Eurythermalism of a deep-sea symbiosis system from an enzymological aspect." The University of Waikato, 2007. http://hdl.handle.net/10289/2588.

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The recently proposed and experimentally validated Equilibrium Model provides the most detailed description of temperature's effect on enzyme catalytic activity to date. By introducing an equilibrium between Eact, the active form of enzyme, and Einact, a reversibly inactivated form of enzyme, the Equilibrium Model explains apparent enzyme activity loss at high temperatures that cannot be accounted for by irreversible thermal denaturation. The Equilibrium Model describes enzyme behavior in the presence of substrates and under assay conditions; thus its associated parameters, deltaHeq and Teq,

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5

Trinconi, Cristiana de Melo. "Investigação sobre a atividade de Ceramida Sintase em Leishmania amazonensis." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-13022012-091618/.

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A leishmaniose é uma doença parasitária de ampla distribuição e de difícil tratamento. Recentemente foi identificada a atividade leishmanicida de tamoxifeno, levando à proposta de sua utilização como alternativa para o tratamento de leishmaniose. Neste trabalho, propusemo-nos a caracterizar a atividade de ceramida sintase (CerS) em L. amazonensis e testar a interferência do tamoxifeno nesta enzima. Identificamos, no genoma de L. amazonensis, uma ORF que codifica uma proteína similar à CerS de Saccharomyces cerevisiae, com seis domínios transmembrana e um motivo Lag1 característico. A caracteri

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6

Eatock, Susan A. (Susan Amelia) Carleton University Dissertation Chemistry. "Activation and reconstitution studies of the enzyme L-Phenylalanine Hydroxylase." Ottawa, 1989.

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7

Valdivia, Ciro Pablo Kopp. "Pruebas de elaboracion de leche de soya (Glycine max (L.) Merril) y derivados proyecto de viabilidad industrial /." Diss., Cochabamba, 1998. http://contentdm.lib.byu.edu/u?/Benson,4195.

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Valdivia, Ciro Pablo Kopp. "Pruebas de elaboracion de leche de soya (Glycine max (L.) Merril) y derivados proyecto de viabilidad industrial /." La Paz, 1997. http://www.lib.byu.edu/valdivia.

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9

Celik, Haydar. "Enzyme-catalyzed Reductive Activation Of Anticancer Drugs Idarubicin And Mitomycin C." Phd thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609247/index.pdf.

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Idarubicin (IDA) and mitomycin C (MC) are clinically effective quinone-containing anticancer agents used in the treatment of several human cancers. Quinone-containing anticancer drugs have the potential to undergo bioreduction by oxidoreductases to reactive species, and thereby exert their cytotoxic effects. In the present study, we investigated, for the first time, the potential of IDA, in comparison to MC, to undergo reductive activation by NADPH-cytochrome P450 reductase (P450R), NADH-cytochrome b5 reductase (b5R) and P450R-cytochrome P4502B4 (CYP2B4) system by performing both in vitro plas

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10

Lai, Chung-Jeng. "Fumarate Activation and Kinetic Solvent Isotope Effects as Probes of the NAD-Malic Enzyme Reaction." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc278864/.

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The kinetic mechanism of activation of the NAD-malic enzyme by fumarate and the transition state structure for the oxidation malate for the NAD-malic enzyme reaction have been studied. Fumarate exerts its activating effect by decreasing the off-rate for malate from the E:Mg:malate and E:Mg:NAD:malate complexes. The activation by fumarate results in a decrease in K_imalate and an increase in V/K_malate by about 2-fold, while the maximum velocity remains constant. A discrimination exists between active and activator sites for the binding of dicarboxylic acids. Activation by fumarate is proposed

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Книги з теми "Enzyme activation"

1

H, Stenmark, ed. Phosphoinositides in subcellular targeting and enzyme activation. Springer, 2004.

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2

Stenmark, Harald, ed. Phosphoinositides in Subcellular Targeting and Enzyme Activation. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18805-3.

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3

Alan, Wiseman, ed. Enzyme induction, mutagen activation, and carcinogen testing in yeast. E. Horwood, 1987.

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4

Mordechai, Liscovitch, ed. Signal-activated phospholipases. R.G. Landes Co., 1994.

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5

H, Schmid-Schönbein, Wurzinger L. J, Zimmermann R. E, and Commission of the European Communities. Committee on Medical and Public Health Research., eds. Enzyme activation in blood-perfused artificial organs: Proceedings of an interdisciplinary meeting. Nijhoff, 1985.

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6

Light, P. E. An investigation into factors controlling activation of the enzyme protein Kinase C in the regulation of neurotransmitter release at the frog neuromuscular junction. University of Birmingham, 1990.

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7

Chen, Chang-Hwei. Activation and Detoxification Enzymes. Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-1049-2.

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8

Chen, Chang-Hwei. Activation and Detoxification Enzymes. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-55287-8.

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9

Zdeněk, Deyl, ed. HPLC in enzymatic analysis. 2nd ed. Wiley, 1998.

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10

1923-, Lorand Laszlo, and Mann Kenneth G, eds. Proteolytic enzymes in coagulation, fibrinolysis, and complement activation. Academic Press, 1993.

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Частини книг з теми "Enzyme activation"

1

Yang, Yanhui, Yu Chen, Herve Aloysius, Daigo Inoyama, and Longqin Hu. "ENZYMES AND TARGETED ACTIVATION OF PRODRUGS." In Enzyme Technologies. John Wiley & Sons, Inc, 2013. http://dx.doi.org/10.1002/9781118739907.ch5.

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2

Chen, Chang-Hwei. "Diets Rich in Enzyme Modulators." In Activation and Detoxification Enzymes. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4614-1049-2_11.

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3

Chen, Chang-Hwei. "Diversified Classes of Enzyme Modulators." In Activation and Detoxification Enzymes. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4614-1049-2_16.

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4

Chen, Chang-Hwei. "Diversified Classes of Enzyme Modulators." In Activation and Detoxification Enzymes. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-55287-8_16.

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5

Chen, Chang-Hwei. "Metabolic Enzyme Induction for Health Benefits." In Activation and Detoxification Enzymes. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-55287-8_18.

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6

Scheller, Frieder, Ulla Wollenberger, Florian Schubert, Dorothea Pfeiffer, Alexander Makower, and C. McNeil. "Multienzyme Biosensors — Coupled Enzyme Reactions and Enzyme Activation." In Uses of Immobilized Biological Compounds. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1932-0_17.

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7

Workman, Paul, and Michael I. Walton. "Enzyme-Directed Bioreductive Drug Development." In Selective Activation of Drugs by Redox Processes. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4615-3768-7_16.

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8

Figarella, C., D. Basso, and O. Guy-Crotte. "Lysosomal Enzyme Activation of Digestive Enzymes During Chronic Pancreatitis?" In Chronic Pancreatitis. Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75319-0_15.

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9

Baici, Antonio. "Multiple Interactions: Essential Activation and Liberation." In Kinetics of Enzyme-Modifier Interactions. Springer Vienna, 2015. http://dx.doi.org/10.1007/978-3-7091-1402-5_7.

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10

Mawson, Raymond, Mala Gamage, Netsanet Shiferaw Terefe, and Kai Knoerzer. "Ultrasound in Enzyme Activation and Inactivation." In Food Engineering Series. Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-7472-3_14.

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Тези доповідей конференцій з теми "Enzyme activation"

1

Matveeva, Valentina, Boris Tikhonov, Daniil Lisichkin, Ajay Desai, and J. C. S. Santos. "STUDY OF PROPERTIES OF GLUCOSE OXIDASE IMMOBILIZED ON MODIFIED WITH CHITOSAN AND SODIUM TRIPOLYPHOSPHATE MAGNETITE." In SGEM International Multidisciplinary Scientific GeoConference 24. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/6.1/s25.29.

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A new biocatalyst based on glucose oxidase immobilized on Fe3O4 magnetite nanoparticles modified with chitosan and sodium tripolyphosphate was synthesized. Magnetite nanoparticles were obtained by mixing solutions of FeCl2 and FeCl3 with ammonia while heating to a temperature of 65 ?C. To stabilize the nanoparticles and ensure the presence of amino groups on their surface, chitosan and sodium tripolyphosphate were sequentially deposited on magnetite. Immobilization of glucose oxidase on the support was carried out after preliminary activation of the carboxyl groups of the enzyme by 1-ethyl-3-(

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2

Wallace, Robert W., E. Ann Tallant, and Lynn M. Brumley. "POSSIBLE ROLE FOR THE CA2+-DEPENDENT PROTEASE (CALPAIN I) AS AN IRREVERSIBLE ACTIVATOR OF CA2+/CALMODULIN-MEDIATED REACTIONS IN THE HUMAN PLATELET." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644528.

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Calmodulin (CaM)-binding proteins have been identified in human platelets using Western blotting techniques and 125I-CaM. Ten proteins of 245, 225. 175, 150, 90. 82(2), 60 and 41(2) kilodaltons (kDa) bind 125I-CaM in a Ca2+-dependent manner; the binding is blocked by both trifluoperazine and nonradiolabeled CaM. The 225 and 90 kDa proteins are labeled by antisera against myosin light chain kinase (MLCK); the 60 kDa and one of the 82 kDa proteins have been identified as the CaM-dependent phosphatase (calcineurin) and caldesmon. The other proteins are presumed to be other Ca2+/CaM regulated enzy

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3

Selak, M. A., M. Chignard, and J. B. Smith. "CHARACTERIZATION OF A NEUTROPHIL CPYMOTRYPSIN-LIKE ENZYME THAT ACTIVATES PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643157.

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Communication between neutrophils and platelets was previously investigated by measuring platelet aggregation, serotonin release and changes in cytosolic free calcium subsequent to specific stimulation of neutrophils by fMet-Leu-Phe (FMLP) in a suspension of both cell types. The addition of the chemotactic peptide was shown to elicit secondary platelet activation as a consequence of primary stimulation of neutrophils. Cell-free supernatants from FMLP-stimulated neutrophils were capable of inducing platelet activation thus demonstrating that a factor released bv neutrophils was responsible for

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4

Noerager, Brett D., Xin Xu, Svetlana Okafor, J. E. Blalock, and Patricia L. Jackson. "Acrolein Treatment Of Human Neutrophils Affects Enzyme Release And Activation." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a2550.

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5

Maschio, A. Del, E. Corvazier, F. Maillet, M. Kazatchkine, and J. Maclouf. "PLATELET-DEPENDENT ACTIVATION AND AMPLIFICATION OF THE POLYMORPHONUCLEAR LEUKOCYTES LYSOSOMAL ENZYME RELEASE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644858.

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The degranulatlon of human PMNs by opsonlsed zymosan (OpZ) was studied In the presence or In the absence of platelet alone or after stimulation by thrombin. Evidence Is presented that the presence of platelets Increased the extent of the liberation of lysozyme from PMNs stimulated by OpZ with a maximal intensity when they were stimulated by thrombin. The extent of the amplification was higher when the PMNs trigger was lower (i.e. 0.5 x 108 particles/ml as compared to 3.0 x 108 particles). This effect was dependent on the platelet concentration (from 10-80 platelets/PMN). Platelets stimulated b

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6

Del Maschio, A., M. Albors, F. Bucchi, et al. "HUMAN POLYMORPHONUCLEAR LEUKOCYTE ACTIVATION INDUCED BY PLATELET ACTIVATING FACTOR (PAF)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643482.

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Human polymorphonuclear leukocytes (PMNs) loaded with the photoprotein Aequorin, were exposed to PAF in the presence of extracellular Ca2+ (1 mM). PMNs aggregation measured In the “Platelet Ionized Calcium Aggregometer” (P.I.C.A.) was dependent on the concentration of the stimulus. Ca2+ cytoplasmatic increase was monitored in parallel at concentrations of PAF which did not modify cellular integrity (10-7-10-5M). The intracellular Ca2+ flux (up to 19±3 µM) triggered by PAF was also concentration-dependent. In order to establish the role played by this intracellular messenger, we studied some ce

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7

Black, Kvar, Mingzhou Zhou, Pinaki Sarder, et al. "Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation." In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X, edited by Samuel Achilefu and Ramesh Raghavachari. SPIE, 2018. http://dx.doi.org/10.1117/12.2301033.

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8

Mannervik, Bengt, and Birgitta I. Sjödin. "Abstract LB-B23: Prodrug activation by designer enzyme targeted to CD123-expressing tumors." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; October 26-30, 2017; Philadelphia, PA. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1535-7163.targ-17-lb-b23.

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9

Collins, Christian, and Christopher J. Ackerson. "Remote enzyme activation using gold coated magnetite as antennae for radio frequency fields." In Colloidal Nanoparticles for Biomedical Applications XIII, edited by Xing-Jie Liang, Wolfgang J. Parak, and Marek Osiński. SPIE, 2018. http://dx.doi.org/10.1117/12.2290478.

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10

Menasni, S., W. Hornebeck, L. Robert, and Y. Legrand. "ELASTASE TYPE ACTIVITY OF ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643360.

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Elastin degrading enzymes have been reported in the vessel wall and both fibroblasts and smooth muscle cells have been shown to produce elastase type enzymes in culture. Data is presented here showing that porcine aortic endothelial cells produce enzyme activities hydrolyzing elastin and synthetic substrates I Sue Ala Ala Ala nitroanilide, SAPNAI considered specific for elastase. Enzyme activity against the SAPNA but not against H-elastin was found to be associated with the cells after triton lysis .This activity was not secreted into the culture medium . The elastolytic activity has been part

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Звіти організацій з теми "Enzyme activation"

1

Wang, Min, and Garry Beuttner. The Enzyme MnSOD Suppresses Malignant Breast Cell Growth by Preventing HIF-1 Activation. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada418673.

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2

Prusky, Dov, Noel Keen, and Rolf Christoffersen. Involvement of Epicatechin in the Regulation of Natural Resistance of Avocado Fruit against Postharvest Pathogens. United States Department of Agriculture, 1997. http://dx.doi.org/10.32747/1997.7613028.bard.

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In this project it was found that the activation of the mechanism of resistance in avocado fruits to Colletotrichum gloeosporioides depends on the increase of the level of the preformed antifungal diene. This increase is regulated by the synthesis of the flavonoid epicatechin present in the fruit peel. Epicatechin is an inhibitor of the enzyme lipoxygenase whose activity catalyze the breakdown of the antifungal diene. Increase in epicatechin concentration inhibits the breakdown of the antifungal compound and since the compound is continuously synthesized, both combined processes result in the

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3

Locy, Robert D., Hillel Fromm, Joe H. Cherry, and Narendra K. Singh. Regulation of Arabidopsis Glutamate Decarboxylase in Response to Heat Stress: Modulation of Enzyme Activity and Gene Expression. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7575288.bard.

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Most plants accumulate the nonprotein amino acid, g-aminobutyric acid (GABA), in response to heat stress. GABA is made from glutamate in a reaction catalyzed by glutamate decarboxylase (GAD), an enzyme that has been shown by the Israeli PI to be a calmodulin (CaM) binding protein whose activity is regulated in vitro by calcium and CaM. In Arabidopsis there are at least 5 GAD genes, two isoforms of GAD, GAD1 and GAD2, are known to be expressed, both of which appear to be calmodulin-binding proteins. The role of GABA accumulation in stress tolerance remains unclear, and thus the objectives of th

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4

Lurie, Susan, John Labavitch, Ruth Ben-Arie, and Ken Shackel. Woolliness in Peaches and Nectarines. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7570557.bard.

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The overall goal of the research was to understand the processes involved in the development of woolliness in peaches and nectarines. Four specific hypotheses were proposed and in the course of the research evidence was gathered t support two of them and to not support two others. The hypotheses and a summary of the evidence are outlined below. 1. That woolliness arises from an imbalance between the activities of the cell wall pectin degrading enzymes. Using 'Flavortop' nectarines and 'Hermoza' peaches as model systems, storage regimes were manipulated to induce or prevent woolliness. The expr

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5

Hodges, Thomas K., and David Gidoni. Regulated Expression of Yeast FLP Recombinase in Plant Cells. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7574341.bard.

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Research activities in both our laboratories were directed toward development of control of the FLP/frt recombination system for plants. As described in the text of the research proposal, the US lab has been engaged in developing regulatory strategies such as tissue-specific promoters and the steroid-inducible activation of the FLP enzyme while the main research activities in Israel have been directed toward the development and testing of a copper-regulated expression of flp recombinase in tobacco (this is an example of a promoter activation by metal ions). The Israeli lab hat additionally com

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6

นุชประยูร, อิศรางค์, มณฑน์มาศ สุนทราวัฒน์ та ธัญณิชา อ่อนดี. การศึกษาโปรตีน ที่สำคัญในพิษงูแมวเซาเพื่อนำมาดัดแปลงใช้ประโยชน์ทางการแพทย์ : รายงานการวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.23.

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ปัญหางูพิษกัดเป็นปัญหาทางสาธารณะสุขที่สำคัญของประเทศไทย หนึ่งในงูพิษที่สำคัญในไทยคืองูแมวเซา (Daboia russellii siamensis) งูชนิดนี้พบมากในแถบภาคกลางและภาคตะวันออกของไทย ผู้ที่ถูกงูชนิดนี้กัด มักมีอาการทางระบบเลือด และภาวะไตวายเฉียบพลัน ซึ่งเป็นสาเหตุสำคัญที่ทำให้ผู้ที่ถูกงูแมวเซากัดเสียชีวิต ในปัจจุบันความรู้เกี่ยวกับกลไกการเกิดพิษหลังถูกงูแมวเซากัด รวมทั้งโปรตีนสำคัญในพิษงูแมวเซาที่อาจมีประโยชน์ทางการแพทย์ ยังไม่มีการศึกษาอย่างแน่ชัด การศึกษาองค์ประกอบของพิษงูแมวเซาในเชิงลึก จะช่วยให้เข้าใจกลไกการเกิดพิษ นำไปสู่การรักษาที่มีประสิทธิภาพที่ดีขึ้น พร้อมทั้งอาจนำความรู้ที่ได้ไปใช้ประโยชน์ในทางการ

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7

นุชประยูร, อิศรางค์. การศึกษาโปรตีนที่สำคัญในพิษงูแมวเซาเพื่อนำมาดัดแปลงใช้ประโยชน์ทางการแพทย์ : รายงานการวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์มหาวิทยาลัย, 2008. https://doi.org/10.58837/chula.res.2008.26.

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ปัญหางูพิษกัดเป็นปัญหาทางสาธารณสุขที่สำคัญของประเทศไทย หนึ่งในงูพิษที่สำคัญในไทยคือ งูแมวเซา (Daboia russellii siamensis) งูชนิดนี้พบมากในแถบภาคกลางและภาคตะวันออกของไทย ผู้ที่ถูกงูชนิดนี้กัด มักมีอาการทางระบบเลือด และภาวะไตวายเฉียบพลัน ซึ่งเป็นสาเหตุสำคัญที่ทำให้ผู้ที่ถูกงูแมวเซากัดเสียชีวิต ในปัจจุบันความรู้เกี่ยวกับกลไกการเกิดพิษหลังถูกงูแมวเซากัด รวมทั้งโปรตีนสำคัญในพิษงูแมวเซาที่อาจมีประโยชน์ทางการแพทย์ ยังไม่มีการศึกษาอย่างแน่ชัด การศึกษาองค์ประกอบของพิษงูแมวเซาในเชิงลึก จะช่วยให้เข้าใจกลไกการเกิดพิษ นำไปสู่การรักษาที่มีประสิทธิภาพที่ดีขึ้น พร้อมทั้งอาจนำความรู้ที่ได้ไปใช้ประโยชน์ในทางการ

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8

Sandermann, Heinrich, Duncan Jr., and Thomas M. Lipid-Dependent Membrane Enzymes. Kinetic Modelling of the Activation of Protein Kinase C by Phosphatidylserine. Defense Technical Information Center, 1991. http://dx.doi.org/10.21236/ada302987.

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9

Prusky, Dov, Noel T. Keen, and Stanley Freeman. Elicitation of Preformed Antifungal Compounds by Non-Pathogenic Fungus Mutants and their Use for the Prevention of Postharvest Decay in Avocado Fruits. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7570573.bard.

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Анотація:

C. gloeosporioides attacks unripe avocado fruits in the orchard. Germinated spores produce appressoria that germinate and breach the cuticle, but the resultant subcuticular hyphae become quiescent and do not develop further until fruit is harvested and ripens. Resistance of unripe avocado to attach by C. gloeosporioides is correlated with the presence of fungitoxic concentrations of the preformed antifungal compound, 1-acetoxy-2-hydroxy-4-oxoheneicosa-12, 15 diene in the pericarp of unripe fruits. The objective of this proposal was to study the signal transduction process by which elicitors in

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10

Freeman, Stanley, and Russell J. Rodriguez. The Interaction Between Nonpathogenic Mutants of Colletotrichum and Fusarium, and the Plant Host Defense System. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573069.bard.

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The intent of this proposal was to study the interaction between nonpathogenic mutants of Colletotrichum magna and Fusarium oxysporum, and the cucurbit host defense system. We had shown previously that a nonpathogenic endophytic mutant path- 1 of C. magna, caused no visible disease symptoms but protected watermelon seedlings from disease caused by the wildtype isolate and F. o. niveum. Objectives were: 1) Determine the microscopic, biochemical and molecular genetic interaction between "protected" (path- 1 colonized) cucurbit hosts and wildtype isolates of C. magna; 2) Isolate non-pathogenic mu

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