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Добірка наукової літератури з теми "Enzyme linked immunosorbent assay kit"

Автор: Grafiati
Опубліковано: 10 січня 2023
Оновлено: 28 січня 2023

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Статті в журналах з теми "Enzyme linked immunosorbent assay kit"

1

Zhang, Hui Ying, and Jun Ping Wang. "Enzyme-Linked Immunosorbent Assay for Streptomycin in Animal Feeds." Advanced Materials Research 651 (January 2013): 280–83. http://dx.doi.org/10.4028/www.scientific.net/amr.651.280.

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Polyclonal antibody against streptomycin was prepared by using a streptomycin–bovine serum albumin conjugate for the immunization of rabbits. Using this antibody, we developed quantitative assays for streptomycin by means of an indirect competitive enzyme-linked immunosorbent assay (icELISA). Fifty percent inhibition concentration (IC50) for the antibody was 3.6 ng/ml. The detection limit was 0.4 ng/ml. The average of recoveries for all samples was 86.14% and the coefficients of variation of intra- and inter-assays were below 18%. The detection limit using the kit was 15 ng/ml in animal feeds.
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2

Madeali, Mun Imah, and Nurhidayah Nurhidayah. "KIT ENZYME-LINKED IMMUNOSORBENT ASSAY UNTUK DETEKSI WSSV PADA UDANG." Jurnal Riset Akuakultur 6, no. 1 (April 30, 2011): 131. http://dx.doi.org/10.15578/jra.6.1.2011.131-137.

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Komponen dasar yang penting dan menentukan keberhasilan pengendalian suatu penyakit dalam bidang perikanan adalah informasi tentang patogen secara dini, cepat, dan akurat, serta epidemi penyakit di lapangan. Teknik serologi, khususnya ELISA, merupakan salah satu teknik yang menjanjikan untuk keperluan tersebut, karena relatif mudah dan murah, serta berpeluang untuk digunakan secara langsung di lapangan. Telah dilakukan uji lapang terhadap perangkat Kit ELISA yang telah diproduksi oleh Balai Riset Perikanan Budidaya Air Payau. Hasil uji lapang menunjukkan bahwa Kit ELISA dapat digunakan untuk mendeteksi penyakit WSSV pada udang windu, dengan waktu ± 3 jam untuk setiap kali pengujian mulai persiapan sampai pembacaan hasil uji. Hasil Kit ELISA lebih cepat dibandingkan dengan metode PCR yang memerlukan waktu 24 jam. Biaya yang diperlukan untuk mendeteksi satu sampel juga lebih ekonomis yaitu ± Rp 35.000,- dibandingkan dengan PCR (Rp 150.000,- – Rp 300.000,-) per sampel
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3

Gabrovská, Dana, Jana Rysová, Vanda Filová, Jan Plicka, Petr Cuhra, Martin KubíK,, Soňa BaršOvá, et al. "Gluten Determination by Gliadin Enzyme-Linked Immunosorbent Assay Kit: Interlaboratory Study." Journal of AOAC INTERNATIONAL 89, no. 1 (January 1, 2006): 154–60. http://dx.doi.org/10.1093/jaoac/89.1.154.

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Abstract An interlaboratory study with 10 participants was performed to obtain validation and performance data for an enzyme-linked immunosorbent assay (ELISA) kit developed for quantitative gluten determination in foods. The ELISA kit used for this study is based on 2 monoclonal and 1 polyclonal antibody developed by Immunotech, a Beckman Coulter Co. This kit did not show any false positive results or cross-reactivity with oat, rice, maize, and buckwheat. The gliadin standard from the Working Group on Prolamin Analysis and Toxicity was included in the kit as reference material for calibration. All participants obtained a gliadin ELISA kit with Standard Operational Procedure and a form for recording test results. The study included 13 samples labeled as gluten-free and 2 samples spiked by wheat flour. Seven samples had gliadin content below the limit of quantitation (LOQ) of the method, and 1 sample exceeded the highest calibration level. Gliadin content in the range from 10 to 157 mg/kg (1st day) and from 11 to 183 mg/kg (2nd day) was found in 7 samples (including 2 spiked samples). Results of these samples were used for further statistical analysis and evaluation. The Cochran, Dixon, and Mandel statistical tests were applied for detection of outliers. The LOQ of the kit was estimated.
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GARBER, ERIC A. E. "Detection of Melamine Using Commercial Enzyme-Linked Immunosorbent Assay Technology." Journal of Food Protection 71, no. 3 (March 1, 2008): 590–94. http://dx.doi.org/10.4315/0362-028x-71.3.590.

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Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. To meet this need, commercial enzyme-linked immunosorbent assay (ELISA) test kits for the detection of triazines were evaluated. The recently released Melamine Plate kit (Abraxis, Warminster, Pa.) displayed a limit of detection of 9 ng/ml for melamine in phosphate-buffered saline (PBS) and approximately 1 μg/ml for melamine added to dog food. An atrazine ELISA test kit produced by Abraxis required 0.2 mg/ml to generate a response more than four times the standard deviation from background. In contrast, with the EnviroGard Triazine Plate kit (Strategic Diagnostics, Inc., Newark, Del.), 1.5 mg/ml melamine in PBS generated a signal only one standard deviation from background, which was insufficient to define a limit of detection. Extraction based on dilution with 105 mM sodium phosphate/75 mM NaCl/2.5% nonfat milk/0.05% Tween 20 (UD) enabled detection of fivefold less melamine in dog food than did use of the procedure recommended by the manufacturer, which entailed extraction into 60% methanol, sonication, centrifugation, filtration, and further dilution into 10% methanol/PBS. Using the Abraxis Melamine ELISA, both extraction protocols yielded identical results with a dog food sample adulterated with mela-mine. The recovery of melamine spiked into gravy from dog food using UD was 74% ± 4%. In conclusion, the recently released Abraxis ELISA for melamine proved to be a useful alternative to more cumbersome methods.
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Štumr, František, Dana Gabrovská, Jana Rysová, Petr Hanák, Jan Plicka, Kvta Tomková, Petr Cuhra, et al. "Enzyme-Linked Immunosorbent Assay Kit for Beta-Lactoglobulin Determination: Interlaboratory Study." Journal of AOAC INTERNATIONAL 92, no. 5 (September 1, 2009): 1519–25. http://dx.doi.org/10.1093/jaoac/92.5.1519.

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Abstract An interlaboratory study was performed in six laboratories to prove the validation of the ELISA method developed for quantitative determination of beta-lactoglobulin (BLG) in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. In-house validation of the kit did not produce false-positive results or cross-reactivity in a broad range of food matrixes containing no milk proteins. All participants obtained the BLG kit with a standard operational procedure, the list of the samples, samples, and a protocol for recording test results. The study included 14 food samples (extruded breakfast cereals, bread, two soy desserts, butter, chicken ham, chicken meat, wheat flour, long grain rice, jelly, two whey drinks, crackers, and bitter chocolate) and six spiked samples (two rice, two wheat flour, and two chicken meat). Nine samples of food matrixes containing no milk proteins showed BLG content lower than the first standard (0.15 mg/kg). Two samples of food matrixes with no milk proteins revealed BLG content higher than standard 3 (1.5 mg/100 g) and standard 4 (5.0 mg/100 g). Three food samples containing milk were tested as positive, and all spiked samples were evaluated as positive. The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as LOQ (0.22 mg BLG/kg) and LOD (0.07 mg BLG/kg), for the kit were calculated.
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Rachmawati, Faidah Rachmawati, I. Wayan Teguh Wibawan, and Ni Luh Putu Ika Mayasari. "Penggunaan Antigen Mycoplasma gallisepticum Freeze-Thawing dalam Teknik Enzyme-Linked Immunosorbent Assay." Jurnal Sain Veteriner 36, no. 2 (January 8, 2019): 151. http://dx.doi.org/10.22146/jsv.27021.

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Chronic Respiratory Disease (CRD) is a bacterial infection in chickens caused by Mycoplasma gallisepticum. CRD may result in economic loss in the livestock industry, decreasing of egg production and feed efficiency, increasing of medication costs, downgrading of carcass and egg qualities. The aim of this study was to develop the Enzyme-Linked Immunosorbent Assay (ELISA) kit for detecting antibodies of M. gallisepticum using freeze-thawing antigens. The cut-off point was determined by S/N method. Chicken antiserum againts M. gallisepticum was collected every week on 1st – 6th week post immunization of whole cell antigen of M. gallisepticum. Optimization of antigen, serum and conjugate were 10 μg/ml protein concentrations, 1:800 serum dilution and 1:10000 conjugate dilution. The validation results on developed ELISA kit (MyGELISA) analyzed by ROC curve using MedCalc statistical software revealed that developed ELISA kit (MyGELISA) had 100% sensitivity and 86.21% specificity with 95% confidence interval. The results of RSA, MyGELISA and commercial ELISA kit showed antibodies positif againts M. gallisepticum. In conclusion, MyGELISA using freeze-thawing antigen can be used to detect antibodies of M. gallisepticum.
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ALSHAIBANI, MUHANNA, Radin Maya Saphira Radin Mohamed, Ishak Mat, Adel AlGheethi, and Jacinta Santhanam. "ENZYME-LINKED IMMUNOSORBENT ASSAY DETECTION FOR MALIGNANCY USING ANTI-P53 ANTIBODIES." Malaysian Journal of Public Health Medicine 21, no. 1 (April 24, 2021): 208–15. http://dx.doi.org/10.37268/mjphm/vol.21/no.1/art.789.

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Tumour protein 53 (p53) plays an important role in the instruction of the cell cycle. In a variety of transformed cell lines, tumour protein is expressed in high amounts, and it is believed to contribute to transformation and malignancy. This research aimed to detect the anti-p53 antibodies in sera of patients with various malignant tumours and to evaluate the sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA). A case-control study was conducted on samples from 49 patients with various types of malignant tumours at Sultanah Bahiyah Hospital, Alor Setar, Kedah, Malaysia, and 32 healthy control cases with non‐malignant disease collected from Universiti Sains Malaysia clinic, Penang, Malaysia. The antibodies against p53 protein in the serum samples were analysed using the commercial ELISA kit, Calbiochem® p53- ELISAPLUS. The results showed that the rate of anti-p53 antibodies in patients with various malignant tumours was 13 out of 49 (26.5 %), compared with only 2 out of 32 (6.25%) in healthy controls (p < 0.001). The sensitivity of this kit reached 28.6% and the specificity was 93.8%. In conclusion, these results suggest that the anti-p53 antibodies can be detected in different sera of malignant tumour patients and the ELISA kit is highly specific; nevertheless, its discrimination power is not perfect because of its low sensitivity to determine the anti-p53 antibodies.
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Collins, Michael T., Arthur Angulo, Claus D. Buergelt, Steve G. Hennager, Sharon K. Hietala, Richard H. Jacobson, Diana L. Whipple, and Robert H. Whitlock. "Reproducibility of a Commercial Enzyme-Linked Immunosorbent Assay for Bovine Paratuberculosis among Eight Laboratories." Journal of Veterinary Diagnostic Investigation 5, no. 1 (January 1993): 52–55. http://dx.doi.org/10.1177/104063879300500112.

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Interlaboratory reproducibility of an absorbed enzyme-linked immunosorbent assay (ELISA) kit for detection of bovine serum antibodies to Mycobacterium paratuberculosis was evaluated. A panel of 30 bovine sera (15 positives and 15 negatives) was tested in triplicate microtiter wells on each of 2 days at 8 different laboratories. One laboratory had invalid results because of positive or negative serum control optical density (OD) readings beyond the acceptable range specified by the kit. The coefficient of variation (CV) for mean OD values was influenced by low ODs on test negative sera at 2 laboratories, thus the CVs on positive sera were considered a more representative measure of kit reproducibility. Between-well CVs averaged 6.7% ± 2.8% (mean ± standard deviation), and between-day CVs averaged 14.5% ± 9.8% among the 7 laboratories with valid assays on the 15 positive sera. The OD values were converted to positive or negative classifications for each assay well, and the results were compared. Among 1,392 assays in 7 laboratories, 98.6% were in agreement. Eleven of 18 discrepant results were due to a sample that consistently gave OD values near the cutoff for a positive test. Exclusion of that serum from the analysis resulted in a 99.8% rate of agreement among laboratories. Results indicated that the absorbed ELISA kit provided reproducible results within and between laboratories.
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Trombley, Arthur, Titan Fan, and Robert LaBudde. "Aflatoxin Plate Kit." Journal of AOAC INTERNATIONAL 94, no. 5 (September 1, 2011): 1519–30. http://dx.doi.org/10.1093/jaoac/94.5.1519.

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Abstract The level of total aflatoxin contamination was analyzed in naturally contaminated and spiked samples of corn and peanut using the Aflatoxin Plate Kit. This kit is an enzyme-linked immunosorbent assay (ELISA) suitable for rapid testing of grains and peanuts. The assay was evaluated for ruggedness and linearity of the standard curve. The test kit results were then statistically evaluated for accuracy, precision, and correlation to a validated HPLC method (AOAC 994.08). The results were verified by an independent laboratory.
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Sakai, Shinobu, Reiko Adachi, Hiroshi Akiyama, Reiko Teshima, Hirotoshi Doi, Haruki Shibata, Atsuo Urisu, et al. "Determination of Walnut Protein in Processed Foods by Enzyme-Linked Immunosorbent Assay: Interlaboratory Study." Journal of AOAC INTERNATIONAL 93, no. 4 (July 1, 2010): 1255–61. http://dx.doi.org/10.1093/jaoac/93.4.1255.

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Abstract Because food allergens from tree nuts, including walnuts, are a frequent cause of adverse food reactions for allergic patients, the labeling of foods containing ingredients derived from tree nuts is required in numerous countries. According to Japanese regulations, the labeling of food products containing walnuts is recommended. To ensure proper labeling, a novel sandwich ELISA kit for the determination of walnut protein in processed foods (Walnut Protein [2S-Albumin] Kit; Morinaga Institute of Biological Science, Inc.; walnut kit) has been developed. We prepared seven types of incurred samples (model processed foods: biscuits, bread, sponge cake, orange juice, jelly, chicken meatballs, and rice gruel) containing 10 g walnut soluble protein/g of food for use in interlaboratory evaluations of the walnut kit. The walnut kit displayed sufficient reproducibility relative standard deviations (interlaboratory precision: 5.89.9 RSDR) and a high level of recovery (81119) for all the incurred samples. All the repeatability relative standard deviation (RSDr) values for the incurred samples that were examined were less than 6.0. The results of this interlaboratory evaluation suggested that the walnut kit could be used as a precise and reliable tool for determination of walnut protein in processed foods.
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Більше джерел

Дисертації з теми "Enzyme linked immunosorbent assay kit"

1

Kleiner, Eric J. "Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA) Test Kits for the Quantitative Determination of Endocrine Disrupting Compounds (EDCs) in Aqueous Phase Environmental Samples." University of Cincinnati / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1282056078.

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2

Mello, Marcelle Bral de. "Padronização do Kit de ELISA Recombinante para o diagnóstico da doença de Chagas visando sua utilização nos Serviços de Hemoterapia." Instituto de Tecnologia em Imunobiológicos, 2009. https://www.arca.fiocruz.br/handle/icict/5875.

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Анотація:
Submitted by Priscila Nascimento (pnascimento@icict.fiocruz.br) on 2012-11-27T12:04:45Z No. of bitstreams: 1 marcelle-bral-de-mello.pdf: 1087389 bytes, checksum: 74eb4d07ffe50812d0cea3fd71cdf47a (MD5)
Made available in DSpace on 2012-11-27T12:04:45Z (GMT). No. of bitstreams: 1 marcelle-bral-de-mello.pdf: 1087389 bytes, checksum: 74eb4d07ffe50812d0cea3fd71cdf47a (MD5) Previous issue date: 2009
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Bio-Manguinhos produz, desde a década de 80, Kits para diagnóstico, inclusive para o diagnóstico da doença de Chagas. O kit EIE-Recombinante-Chagas, que utiliza na sua composição as proteínas recombinantes CRA e FRAdo T. cruzi, foi registrado como produto junto à ANVISA no ano de 1999. No período de renovação do registro, o Kit passou por uma avaliação prévia no INCQS, onde não foi possível alcançar condição satisfatória quanto aos níveis de sensibilidade. O presente trabalho teve por objetivo retomar a padronização deste Kitno âmbito do desenvolvimento tecnológico. Para tanto foi estabelecido a padronização de três modelos de protótipos, utilizando variações das construções disponíveis das proteínas recombinantes CRA e FRA, na forma de apresentação como antígeno e como conjugado. Posteriormente, foi avaliado o desempenho de cada protótipo, em três diferentes fases. Na primeira fase, o melhor desempenho foi apresentado pelo protótipo 3, cujos níveis de sensibilidade e especificidade foram respectivamente de 99% e 99,5% (IC 95%). Na segunda fase o nível de sensibilidade do protótipo 3 foi de 95.8% e o de especificidade 100%, mantendo a melhor condição de desempenho em relação aos demais. Na última fase, somente o protótipo 3 foi avaliado em função da indisponibilidade de volume das amostras selecionadas e os níveis de sensibilidade e especificidade encontrados de 100%.O desempenho do protótipo 2 foi insatisfatório nas duas primeiras fases de avaliação.Tendo em vista o bom desempenho alcançado pelo protótipo 3, propomos a continuação deste modelo na elaboração futura de lotes-piloto, de estudos multicêntricos e de validação, em ações conjuntas com o controle e a garantia da qualidade para a elaboração de documentação visando pedido de registro desse produto junto aos órgãos regulatórios.
Bio-Manguinhos produces, since the 80’s, a variety of kits for diagnosis, including the kit for Chagas’ disease diagnostic. The EIE-Recombinant-Chagas’ kit, which has in its constitution the recombinant proteins CRA and FRA from T. cruzi, was registered at ANVISA as a product in 1999. During the renewal of the registration period, the kit was submitted to a previous registration analysis by The National Institute for Quality Control in Health (INCQS), when it did not show satisfactory sensitivity levels. The present work aimed to remake the kit standardization process under the scope of the technological development. In order to do it, we established the standardization of three prototype models, each one using variations of available constructions from CRA and FRA recombinant proteins in the form of presentation as antigen and as conjugate. Afterwards, we evaluated the performanceof each prototype, in three different stages. In the first one, prototype 3 showed the best performance with 99% sensitivity and 99.5% specificity levels (CI 95%). In the second stage, prototype 3 levels sensitivity and specificity were 95.8% and 100% respectively, keeping the best performance condition as compared to the other two prototypes. In the last stage, only prototype 3 was assessed due to the lack of sample volumes. The reported sensitivity and specificity levels forsuch prototype were equal to 100%. The performance of prototype 2 was unsatisfactory in the first two assessment stages. Taking into account the performance achieved by prototype 3, we propose the continuation of this model for future development of pilot lots, multicenter and validation studies, in joint actions with quality assurance and quality control for the arrangement of the documents requested by the National Regulatory Agency for the registration of the product.
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3

Andersson, Josefin. "Utvärdering av Malaria Antigen ELISA kit för diagnostik av malaria vid Christian Medical College and Hospital i Vellore, Indien. : en jämförande studie mellan Quantitative buffy coat och enzyme-linked immunosorbent assays (ELISA) metodik." Thesis, Örebro universitet, Institutionen för hälsovetenskap och medicin, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-4889.

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Malaria är ett globalt hälsoproblem som orsakar många dödsfall runt om i världen varje år och nästan hälften av jordens befolkning ligger i riskzonen att drabbas av sjukdomen. I Indien drabbas mellan 2-3 miljoner människor varje år och det inträffar omkring 900 dödsfall. Malaria orsakas av Plasmodium sp. som är en protozoe, och det finns fyra olika arter som är patogena för människor, P. vivax, P. ovale, P. falciparium samt P. malariae. Vanliga metoder för att diagnostisera malaria är genom tunna och tjocka blodutstryk som färgas till exempel med Giemsa, Fields eller Leishmans färgningsteknik och studeras mikroskopiskt, Quantitative Buffy Coat (QBC), PCR tester, acridinorange färgning samt olika immunologiska tester för detektion av antikroppar eller antigen som till exempel enzyme-linked immunosorbent assays (ELISA) test och dipstick test. Syftet med denna studie är att utvärdera om en användning av SD Bio Line Malaria Antigen ELISA kit ger en mer känslig, tillförlitlig, praktisk samt mindre kostsam diagnostikmetod för malaria hos patienter med misstänkt malariainfektion än den nuvarande guldstandardmetoden, QBC tillsammans med blodutstryk, vid Christian Medical College and Hospital i Vellore. Patientproverna har i både ELISA testet samt QBC testet tillsammans med utstryk erhållit samma resultat vilket tyder på att SD Bio Line Malaria Antigen ELISA kitet skulle kunna vara en lika bra diagnostikmetod som QBC testet för diagnos av malaria. ELISA kitet har dock fler nackdelar, i jämförelse med QBC testet, så därför är slutsatsen att SD Bio Line Malaria Antigen ELISA kitet inte är en mer lämplig diagnostisk metod för malaria än den som används vid CMCH. Men då ELISA testet ändå ger en säker diagnos, enligt resultatet i studien, kan den vara ett lämpligt test inom något annat användningsområde.
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4

Wesolowski, Eva. "Monoclonal antibody enzyme-linked immunosorbent assay for carcinoembryonic antigen." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66230.

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5

Kiote-Schmidt, Chrissoula. "Etablierung eines kompetitiven enzymgekoppelten Immunoassays zum Nachweis eines kleinen Peptids in Serum- und Liquorproben." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-59192.

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6

Kim, In Soo. "A quantitative enzyme linked immunosorbent assay for polychlorinated biphenyls in transformer oil." Thesis, Cranfield University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323838.

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7

Meindel, Mandy J. "Enzyme-linked immunosorbent assay to measure serum ferritin in toucans (Ramphastidae sp.)." Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/18896.

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Анотація:
Master of Science
Department of Diagnostic Medicine/ Pathobiology
Lisa M. Pohlman
Background: Iron storage disease has proven to be a serious health concern for captive toucans. Physiologic mechanisms to efficiently extract iron from naturally iron-deficient diets appear the likely cause of iron overload when fed iron-sufficient diets in captivity. Iron overload can result in diabetes, heart failure, and even death. Serum ferritin concentrations are considered the most reliable screening tool to predict total body iron stores in many species, but an assay has not been available to measure serum ferritin in toucans. Objective: The purpose of this study was to develop an enzyme-linked immunosorbent assay (ELISA) to measure serum ferritin in toucans using a polyclonal antibody in a sandwich arrangement. Methods: Ferritin was isolated from toucan liver and used as a standard. A rabbit polyclonal anti-toucan antibody was used as the capture antibody and as a detection antibody conjugated to horseradish peroxidase. Linearity of toucan ferritin standards, effect of serum dilution, recovery of added ferritin standards, and intra- and inter-assay variability were determined. Results: Ferritin standards were linear from 0 to 50 ng/ml. The relationship between serum dilution and serum ferritin concentration was also linear. When 10, 20, 30, 40, or 50 ng/ml of purified toucan ferritin were added to diluted serum, the recoveries varied from 69% to 104%. The intra-assay variability for four test serum samples averaged 11% and the inter-assay variability for the same four samples averaged 11%. Conclusions: Although the results from the linearity and recovery studies are promising for assay development when viewed independently, preliminary ferritin concentrations from all toucans studied are much higher than expected. Upon further evaluation including Dot blot assays, Western blot assays, SDS-PAGE, and protein determination of the ferritin stock solution, it was determined that the ferritin stock solution did not contain a pure protein and therefore likely renders the assay invalid. Further testing is needed to confirm these findings.
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8

Leung, Sau-man Sally. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2337309X.

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9

Carroll, Andrea D. "Development of bead injection methodology for immunoassays /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/8599.

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10

Kwok, Siu Kwong Alan. "Enzyme-linked immunosorbent assay development for advanced glycation end products and brochocin-C." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22621.pdf.

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Книги з теми "Enzyme linked immunosorbent assay kit"

1

Hosseini, Samira, Patricia Vázquez-Villegas, Marco Rito-Palomares, and Sergio O. Martinez-Chapa. Enzyme-linked Immunosorbent Assay (ELISA). Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-6766-2.

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The ELISA guidebook. 2nd ed. New York, NY: Humana Press, 2009.

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3

M, Cambra Alvarez, International Office of Epizootics, and Instituto Nacional de Investigaciones Agrarias., eds. Enzyme immunoassay techniques, ELISA, in animal and plant diseases. 2nd ed. Paris, France: Office international des épizooties, 1987.

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4

A practical guide to ELISA. Oxford [England]: Pergamon Press, 1991.

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5

Calamel, Michel. E.L.I.S.A. standardised technique. Nice, France: Laboratoire national de pathologie des petits ruminants et des abeilles, 1988.

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6

M, Kemeny D., ed. ELISA and other solid phase immunoassays: Theoretical and practical aspects. Chichester: J. Wiley, 1988.

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7

The ELISA guidebook. Totowa, NJ: Humana Press, 2001.

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8

ELISA: Theory and practice. Totowa, N.J: Humana Press, 1995.

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9

World Health Organization. Working Group on Rapid Diagnostic Methods for M. leprae Infection. Report: Meeting of the Working Group on Rapid Diagnostic Methods for M. leprae Infection. Manila, Philippines: The Office, 1986.

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10

Rothwell, E. An assessment of the value of IgM antibody to Hepatitis C virus using synthetic peptide enzyme immunoassays. Dublin: University College Dublin, 1997.

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Частини книг з теми "Enzyme linked immunosorbent assay kit"

1

Cooley, Laura A., Daniel G. Bausch, Marija Stojkovic, Waldemar Hosch, Thomas Junghanss, Marija Stojkovic, Waldemar Hosch, et al. "Enzyme-Linked Immunosorbent Assay." In Encyclopedia of Intensive Care Medicine, 877. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-00418-6_3099.

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2

Dijkstra, Jeanne, and Cees P. de Jager. "Enzyme-Linked Immunosorbent Assay." In Practical Plant Virology, 348–62. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-72030-7_56.

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3

Töpfer, G. "Enzyme-linked Immunosorbent Assay." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_1013-1.

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Töpfer, G. "Enzyme-linked Immunosorbent Assay." In Springer Reference Medizin, 789–90. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_1013.

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5

Jordan, William J. "Enzyme-Linked Immunosorbent Assay." In Medical Biomethods Handbook, 419–27. Totowa, NJ: Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:419.

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Bandopadhyay, Ritam, Suraj Singh S. Rathod, and Awanish Mishra. "Enzyme-Linked Immunosorbent Assay." In Analysis of Naturally Occurring Food Toxins of Plant Origin, 215–44. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003222194-17.

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Crowther, John. "Enzyme Linked Immunosorbent Assay (ELISA)." In Springer Protocols Handbooks, 657–82. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_37.

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Crowther, John R. "Enzyme- Linked Immunosorbent Assay (ELISA)." In Springer Protocols Handbooks, 595–617. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_46.

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Gooch, Jan W. "Enzyme-Linked Immunosorbent Assay (ELISA)." In Encyclopedic Dictionary of Polymers, 891. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13676.

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10

Jordan, William. "Competitive Enzyme-Linked Immunosorbent Assay." In Immunochemical Protocols, 215–25. Totowa, NJ: Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-873-0:215.

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Тези доповідей конференцій з теми "Enzyme linked immunosorbent assay kit"

1

Kunhipurayil, Hasna, Muna Ahmed, and Gheyath Nasrallah. "West Nile Virus Seroprevalence among Qatari and Immigrant Populations within Qatar." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0197.

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Background: West Nile virus (WNV) is one of the most widely spread arboviruses worldwide and a highly significant pathogen in humans and animals. Despite frequent outbreaks and endemic transmission being reported in the Middle East and North Africa (MENA), seroprevalence studies of WNV in Qatar are highly lacking. Aim: This study aims to investigate the actual prevalence of WNV among local and expatriate communities in the Qatar using a large sample size of seemingly healthy donors. Method: A total of 1992 serum samples were collected from donors of age 18 or older and were tested for the presence of WNV antibodies. Serion enzyme-linked immunosorbent assay (ELISA) commercial microplate kits were used to detect the presence of the WNV IgM and IgG. The seropositivity was statistically analyzed using SPSS software with a confidence interval of 95%. Results: The seroprevalence of anti-WNV IgG and IgM in Qatar was 10.3% and 3.4%, respectively. The country-specific seroprevalence according to nationality for WNV IgG and IgM, respectively, were Sudan (37.0%, 10.0%), Egypt (31.6%, 4.4%), India (13.4%, 3.2%), Yemen(10.2%, 7.0%), Pakistan (8.6%, 2.7%), Iran (10.6%, 0.0%), Philippines (5.4%, 0.0%), Jordan(6.8%, 1.1%), Syria (2.6%, 9.6%), Palestine (2.6%, 0.6%), Qatar (1.6%, 1.7%), and Lebanon (0.9%, 0.0%). The prevalence of both IgM and IgG was significantly correlated with the nationality (p≤0.001). Conclusion: Among these tested nationalities, Qatar national has a relatively low burden of WNV disease. The highest prevalence of WNV was found in the Sub Saharan African nationalities like Sudan and Egypt. The seroprevalence of WNV is different from the previously reported arboviruses such as CHIKV and DENV, which was highest among Asian countries (India and Philippines). Further confirmatory tests such as viral neutralization assays are needed to confirm the IgM seropositivity in these samples since these samples could be a source of viral transmission through blood donation.
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Ananda, Alfie Rizky, Donny Danudirdjo, Agung W. Setiawan, and Tati Latifah R. Mengko. "Development of LED Based Enzyme-Linked Immunosorbent Assay Reader." In 2021 IEEE 7th International Conference on Smart Instrumentation, Measurement and Applications (ICSIMA). IEEE, 2021. http://dx.doi.org/10.1109/icsima50015.2021.9526331.

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Sugihara, T., J. Takamatsu, T. Kamiya, H. Saito, K. Kimata, and K. Kato. "A SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR SERUM LAMININ." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643554.

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Laminin, a large glycoprotein, is a major and specific component of basement membrane. There were little or no circulating laminin in normal persons, although some recent reports have showed increased values in various diseases(ex. diabetes mellitus and liver disease) by a radioimmunoassay(RIA) using laminin fragment. The minimum detectable sensitivity of RIA was reported to be 20 ng/ml of serum sample. We describe here a more sensitive immunoassay system, and also the concentrations of laminin in sera from healthy subjects and patients. A sandwich ELISA method for measurement of laminin was established by use of purified antibodies to mouse laminin. The assay system consisted of poly-stylene balls with immobilized antibody F(ab’)2 fragments and the same antibody Fab1 fragments labeled with β-D-galactosidase from E.Coli. The assay was highly sensitive and can detect as small as 0.5 ng/ml of serum laminin.Coefficients of variation in within-run and between-run precision studies for serum laminin were good. Serum laminin levels in healthy subjects of various ages ranged from 1.5 to 3.9 ng/ml(n=60). Fifty eight patient sera (collagen disease(n=18), hepatic disease(n=20), and renal disease (n=20)) were examined. Significant differences between the normal sera and 3 diseases sera were observed as shown belowIt is concluded that circulating laminin apparently exists in normal persons and there are higher laminin level in some diseases which appears to involve basement membrane-rich organ
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Ananda, Alfie Rizky, Donny Danudirdjo, Agung W. Setiawan, and Tati Latifah R. Mengko. "Development of Low Cost Enzyme-Linked Immunosorbent Assay Plate Reader." In 2019 International Symposium on Electronics and Smart Devices (ISESD). IEEE, 2019. http://dx.doi.org/10.1109/isesd.2019.8909658.

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Ishizawa, M., T. Azeta, H. Nose, Y. Ukita, and Y. Utsumi. "Three-dimensional lab-on-a-CD with enzyme-linked immunosorbent assay." In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2012. http://dx.doi.org/10.1109/nems.2012.6196759.

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Sasagawa, Kiyotaka, Soo Heyon Kim, Kazuya Miyazawa, Hironari Takehara, Toshihiko Noda, Takashi Tokuda, Ryota Iino, Hiroyuki Noji, and Jun Ohta. "Dual-mode lensless imaging device for digital enzyme linked immunosorbent assay." In SPIE BiOS, edited by Benjamin L. Miller, Philippe M. Fauchet, and Brian T. Cunningham. SPIE, 2014. http://dx.doi.org/10.1117/12.2039948.

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Berger, C., A. Pilch, J. Ruppert, FP Armbruster, and J. Stein. "An enzyme-linked immunosorbent assay for therapeutic drug monitoring of Golimumab." In Viszeralmedizin 2017. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1604810.

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Alhumdany, Zahraa, and Yasser Albadrany. "Pharmacokinetic of Flunixin Meglumine in Broiler Chicks by Enzyme-linked Immunosorbent Assay." In 1st International Ninevah Conference on Medical Sciences (INCMS 2021). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/ahsr.k.211012.025.

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Lee, Wen-Wai, Kuan-Ju Tseng, and Ju-Nan Kuo. "PDMS microlens fabricated for compact disk enzyme-linked immunosorbent assay (CD-ELISA) applications." In 2010 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2010). IEEE, 2010. http://dx.doi.org/10.1109/nems.2010.5592184.

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Atanasova, M. K., N. V. Ivanova, and T. I. Godjevargova. "Enzyme-linked immunosorbent assay for determination of aflatoxin M1 based on magnetic nanoparticles." In PROCEEDINGS OF THE 6TH INTERNATIONAL ADVANCES IN APPLIED PHYSICS AND MATERIALS SCIENCE CONGRESS & EXHIBITION: (APMAS 2016). Author(s), 2017. http://dx.doi.org/10.1063/1.4975420.

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Звіти організацій з теми "Enzyme linked immunosorbent assay kit"

1

López-Valverde, Nansi, Antonio López-Valverde, Ana Suarez, Bruno Macedo de Sousa, and Juan Manuel Aragoneses. Association of gastric infection and periodontal disease through Helicobacter pylori as a common denominator: A systematic review and meta-analysi. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, October 2021. http://dx.doi.org/10.37766/inplasy2021.10.0097.

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Review question / Objective: Is gastric helicobacter pylori infection related to periodontal diseases? Condition being studied: Therefore, the aim of this systematic review and meta-analysis was to identify and analyze clinical studies to determine the direct correlation between Helicobacter Pylori gastric infection andPeriodontal Disease. Study designs to be included: Clinical studies that provided data on Helicobacter Pylori infection in both the stomach and oral cavity, confirmed by polymerase chain reaction (PCR), rapid urease test (RUT) or enzyme-linked immunosorbent assay (ELISA). Clinical studies that associated PD with Helicobacter Pylori. The diagnosis of PD was confirmed ac-cording to the diagnostic criteria in periodontology.
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Occurrence and Distribution of Pesticides in the St. Lucie River Watershed, South-Central Florida, 2000-01, Based on Enzyme-Linked Immunosorbent Assay (ELISA) Screening. US Geological Survey, 2003. http://dx.doi.org/10.3133/wri024304.

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