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Готові списки джерел за темами / Enzymes Synthesis

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Добірка наукової літератури з теми "Enzymes Synthesis"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 6 вересня 2023

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Статті в журналах з теми "Enzymes Synthesis"

1

Malá, Š., P. Karasová, M. Marková, and B. Králová. "Oligosaccharide synthesis using a-glucosidases of different origin." Czech Journal of Food Sciences 19, No. 2 (February 7, 2013): 57–61. http://dx.doi.org/10.17221/6576-cjfs.

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a-Glucosidase from Aspergillus awamori and intestinal a-glucosidase (saccharase-isomaltase complex) exhibited high transglycosylation activity and were able to synthesize tri- and tetrasaccharides during maltose hydrolysis. Both tested enzymes were also able to transfer the glucose residue to all tested monosaccharide acceptors (D-mannose, D-xylose, L-sorbose and D-galactose). Their transfer activity towards respective acceptors varied and their acceptor preference also depended on the origin of the enzyme. Out of the acceptors tested, both enzymes exhibited high transfer activity in xylose.

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2

Herman, Richard Ansah, Xuan Zhu, Ellen Ayepa, Shuai You, and Jun Wang. "Advances in the One-Step Approach of Polymeric Materials Using Enzymatic Techniques." Polymers 15, no. 3 (January 30, 2023): 703. http://dx.doi.org/10.3390/polym15030703.

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The formulation in which biochemical enzymes are administered in polymer science plays a key role in retaining their catalytic activity. The one-step synthesis of polymers with highly sequence-controlled enzymes is a strategy employed to provide enzymes with higher catalytic activity and thermostability in material sustainability. Enzyme-catalyzed chain growth polymerization reactions using activated monomers, protein–polymer complexation techniques, covalent and non-covalent interaction, and electrostatic interactions can provide means to develop formulations that maintain the stability of th

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3

O'Keefe, S. J., W. M. Bennet, A. R. Zinsmeister, and M. W. Haymond. "Pancreatic enzyme synthesis and turnover in human subjects." American Journal of Physiology-Gastrointestinal and Liver Physiology 266, no. 5 (May 1, 1994): G816—G821. http://dx.doi.org/10.1152/ajpgi.1994.266.5.g816.

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Animal studies have shown that pancreatic enzyme secretion is independent of enzyme synthesis. To investigate this relationship in humans, we have coinfused 14C-labeled leucine tracer with cholecystokinin octapeptide in nine healthy adults for 4 h and measured the rate of appearance of secreted and newly labeled enzymes in the duodenum. Enzyme secretion was well maintained throughout, but newly labeled enzymes only appeared in juice between 75 and 101 min (median time, 86 min), indicating that initial secretion was dependent on the release of zymogen stores and that the median production time

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4

Juwon, Arotupin Daniel, and Ogunmolu Funso Emmanuel. "Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation." Biotechnology Research International 2012 (July 15, 2012): 1–8. http://dx.doi.org/10.1155/2012/904763.

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The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented

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5

Hu, Chong, Yunxiu Bai, Miao Hou, Yisu Wang, Licheng Wang, Xun Cao, Chiu-Wing Chan, et al. "Defect-induced activity enhancement of enzyme-encapsulated metal-organic frameworks revealed in microfluidic gradient mixing synthesis." Science Advances 6, no. 5 (January 2020): eaax5785. http://dx.doi.org/10.1126/sciadv.aax5785.

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Mimicking the cellular environment, metal-organic frameworks (MOFs) are promising for encapsulating enzymes for general applications in environments often unfavorable for native enzymes. Markedly different from previous researches based on bulk solution synthesis, here, we report the synthesis of enzyme-embedded MOFs in a microfluidic laminar flow. The continuously changed concentrations of MOF precursors in the gradient mixing on-chip resulted in structural defects in products. This defect-generating phenomenon enables multimodal pore size distribution in MOFs and therefore allows improved ac

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6

Bur, Daniel, Marcel A. Luyten, Hla Wynn, Louis R. Provencher, J. Bryan Jones, Marvin Gold, James D. Friesen, Anthony R. Clarke, and J. John Holbrook. "An evaluation of the substrate specificity and asymmetric synthesis potential of the cloned L-lactate dehydrogenase from Bacillusstearothermophilus." Canadian Journal of Chemistry 67, no. 6 (June 1, 1989): 1065–70. http://dx.doi.org/10.1139/v89-161.

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The potential utility of the L-lactate dehydrogenase of Bacillusstearothermophilus (BSLDH) for stereospecific, preparative-scale reductions of α-keto acids to (S)-α-hydroxy acids of > 99% ee has been demonstrated. BSLDH is a stable, thermophilic, enzyme whose gene has been cloned into a high-expression vector to assure its plentiful supply. Its specificity for keto acid substrates possessing straight- and branched-chain alkyl, cyclopropyl, or phenyl groups has been evaluated in preparative and kinetic terms, and compared with that of the mammalian pig heart enzyme (PHLDH). The specificities

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7

Smith, A. G. "Subcellular localization of two porphyrin-synthesis enzymes in Pisum sativum (pea) and Arum (cuckoo-pint) species." Biochemical Journal 249, no. 2 (January 15, 1988): 423–28. http://dx.doi.org/10.1042/bj2490423.

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The subcellular location of the two porphyrin-synthesis enzymes 5-aminolaevulinate dehydratase (ALAD) and porphobilinogen deaminase (PBGD) was investigated in Pisum sativum (pea) leaves and spadices of Arum (cuckoo-pint). Throughout the tissue-fractionation procedures the distribution of the two enzymes paralleled that of the plastid marker enzyme (ADP-glucose pyrophosphorylase), even in Arum, a tissue where the synthesis of non-plastid haem is predominant. The distribution of cytosolic marker enzyme (lactate dehydrogenase) was significantly different from that of ALAD and PBGD and, although p

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8

Hai guan Ding, Hai guan Ding, Zhi qiang Cai Zhi qiang Cai, Ling Hou Ling Hou, Zhi quan Hu Zhi quan Hu, Zheng sheng Jin Zheng sheng Jin, Di Xu Di Xu, Hui Cao Miao miao Meng Hui Cao Miao miao Meng, Yu Hui Xie Yu Hui Xie, and De qiang Zheng De qiang Zheng. "Synthesis and Evaluation of Some Novel 6-Substituted Quinazoline Derivatives as Antitumor Agents." Journal of the chemical society of pakistan 41, no. 1 (2019): 186. http://dx.doi.org/10.52568/000716/jcsp/41.01.2019.

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A series of novel 6-substituted quinazoline derivatives were synthesised as epidermal growth factor receptor(EGFR) and Human epidermal growth factor receptor 2 (HER2)inhibitors in our lab. The novel compounds were measured for their dual enzyme inhibition as well as their cytotoxic activity on MCF7 cell line. The results revealed that all the compounds showed inhibition of both enzymes. Compound 5c showed the best inhibitory activity against both enzymes and IC50 of its was 2.6 nM against EGFR kinases and 4.3 nM against HER2 kinases, respectively. Most of the measured compounds showed to antit

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9

Morrow, Cary J. "Biocatalytic Synthesis of Polyesters Using Enzymes." MRS Bulletin 17, no. 11 (November 1992): 43–47. http://dx.doi.org/10.1557/s0883769400046650.

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Plants and animals have been exploited as sources of materials for centuries but, as our ability to analyze and fractionate them has progressed, the extraordinary range of properties available from materials produced by living systems has continued to grow. Doi, in another article in this issue of the MRS Bulletin, presents a discussion of a group of naturally occurring polyesters related to poly(beta-hydroxybutyrate). These polyesters are formed in vivo by several microorganisms as part of an energy storage scheme. Research on these systems has allowed growth conditions to be found that can l

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10

Wong, Chi-Huey. "Enzymes for Glycoprotein Synthesis." CHIMIA International Journal for Chemistry 63, no. 6 (June 24, 2009): 318–26. http://dx.doi.org/10.2533/chimia.2009.318.

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Дисертації з теми "Enzymes Synthesis"

1

Ekici, Özlem Doğan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Available online, Georgia Institute of Technology, 2004:, 2003. http://etd.gatech.edu/theses/available/etd-04062004-164633/unrestricted/ekici%5Fozlem%5Fd%5F200312%5Fphd.pdf.

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2

Ekici, Ozlem Dogan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Diss., Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/5333.

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3

Naylor, Neil J. "Biotransformations involving hydrolytic enzymes." Thesis, University of Warwick, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263607.

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4

Silveira, Alvito J. "Synthesis of 6-guanidinobenzoxazinones as potential inhibitors of trypsin-like enzymes." Thesis, Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/26914.

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5

Snider, Catherine E. "Synthesis and biochemical evaluation of irreversible inhibitors of aromatase /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487266362338344.

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6

Carnell, Andrew John. "Cycloalkanone monooxygenase enzymes in organic synthesis." Thesis, University of Exeter, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293971.

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7

Blum, Janna Karen. "Broadening the enyzme-catalyzed synthesis of semi-synthetic antibiotics." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/39528.

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An alpha-amino ester hydrolase (AEH) applicable to synthesis of semi-synthetic antibiotics was cloned from the genomic DNA of Xanthomonas campestris pv. campestris sp. strain ATCC 33913. AEHs catalyze the synthesis and hydrolysis of alpha-amino beta-lactam antibiotics. The enzyme was characterized for thermodynamic and kinetic parameters. The enzyme shows optimal ampicillin hydrolytic activity at 25C and pH 6.8. The AEH enzymes have been shown to have excellent synthetic capability. Additionally, we demonstrated the first fully aqueous enzymatic one-pot synthesis of ampicillin direct from the

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8

Admans, Gary David. "Asymmetric transformations catalysed by lipase enzymes." Thesis, University of Exeter, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240288.

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9

Chambers, Martina Natasha. "Synthesis of cellulosic glycolipids using engineered enzymes." Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/46032.

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Cellulose, a linear polymer of D-glucose units connected by β-1,4 glycosidic bonds, adopts a highly-ordered crystalline structure in solution. In cellulose I, the dominant form of cellulose in nature, the polymeric chains are aligned in the same direction. Previous attempts to synthesize cellulose I in vitro have resulted in the synthesis of cellulose II, which has the thermodynamically favored anti-parallel orientation of chains. The synthesis of soluble fragments or defined surfaces of cellulose I would enable more detailed study of carbohydrate binding domains and other proteins that intera

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10

Ahmed, Naveed. "The synthesis of inhibitors of proteolytic enzymes." Thesis, University of Huddersfield, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416765.

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Книги з теми "Enzymes Synthesis"

1

Kirby, Anthony J. From enzyme models to model enzymes. Cambridge: Royal Society of Chemistry, 2009.

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2

Bednarski, Mark D., and Ethan S. Simon, eds. Enzymes in Carbohydrate Synthesis. Washington, DC: American Chemical Society, 1991. http://dx.doi.org/10.1021/bk-1991-0466.

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3

1958-, Bednarski Mark D., Simon Ethan S. 1963-, American Chemical Society. Division of Carbohydrate Chemistry., and American Chemical Society Meeting, eds. Enzymes in carbohydrate synthesis. Washington, DC: American Chemical Society, 1991.

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4

library, Wiley online, ed. Asymmetric organic synthesis with enzymes. Weinheim: Wiley-VCH, 2008.

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5

Holland, H. L. Organic synthesis with oxidative enzymes. New York: VCH, 1992.

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6

Holland, Herbert L. Organic synthesis with oxidative enzymes. Weinheim: VCH, 1991.

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7

Wong, Chi-Huey. Enzymes in synthetic organic chemistry. Oxford, U.K: Pergamon, 1994.

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8

Ronald, Breslow, ed. Artificial enzymes. Weinheim: Wiley-VCH, 2005.

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9

Schneider, M. P., ed. Enzymes as Catalysts in Organic Synthesis. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4686-6.

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10

P, Schneider M., ed. Enzymes as catalysts in organic synthesis. Dordrecht, Holland: D. Reidel Pub. Co., 1986.

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Частини книг з теми "Enzymes Synthesis"

1

Ullmann, Dirk, and Hans-Dieter Jakubke. "Protease-Catalyzed Peptide Synthesis." In Proteolytic Enzymes, 312–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59816-6_18.

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2

Aehle, Wolfgang, and Juergen Eck. "Discovery of Enzymes." In Enzyme Catalysis in Organic Synthesis, 67–87. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527639861.ch3.

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3

Roberts, S. M. "Enzymes in Organic Synthesis." In Microbial Enzymes and Biotechnology, 395–424. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0765-2_12.

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4

Tiwari, Vinod K., Abhijeet Kumar, Sanchayita Rajkhowa, Garima Tripathi, and Anil Kumar Singh. "Enzymes in Organic Synthesis." In Green Chemistry, 317–52. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-2734-8_8.

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5

Pleiss, Jürgen. "Rational Design of Enzymes." In Enzyme Catalysis in Organic Synthesis, 89–117. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527639861.ch4.

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6

Reetz, Manfred T. "Directed Evolution of Enzymes." In Enzyme Catalysis in Organic Synthesis, 119–90. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527639861.ch5.

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7

Bentley, Paul A. "Synthetic Enzymes-Artificial Peptides in Stereoselective Synthesis." In Biotechnology, 491–517. Weinheim, Germany: Wiley-VCH Verlag GmbH, 2008. http://dx.doi.org/10.1002/9783527620913.ch12.

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8

Turner, N. J. "Asymmetric synthesis using enzymes and whole cells." In Advanced Asymmetric Synthesis, 260–74. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-007-0797-9_13.

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9

Mosbach, Klaus. "Immobilized Enzymes in Organic Synthesis." In Ciba Foundation Symposium 111 - Enzymes in Organic Synthesis, 57–75. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470720929.ch6.

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10

Rozzell, David. "Tabular Survey of Available Enzymes." In Enzyme Catalysis in Organic Synthesis, 1847–938. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527639861.ch46.

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Тези доповідей конференцій з теми "Enzymes Synthesis"

1

Grebennikova, Olga, Aloeksandrina Sulman, and Valentina Matveeva. "SYNTHESIS OF MAGNETICALLY SEPARATED BIOCATALYTIC SYSTEMS." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/6.1/s25.16.

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The use of magnetic nanoparticles in biocatalysis, due to their unique properties, such as controlled particle size, large surface area, and ease of separating them and the reaction mixture by applying an external magnetic field, makes it possible to reuse enzymes immobilized on magnetic nanoparticles for catalytic processes. In this work, horseradish root peroxidase was immobilized on Fe3O4 magnetic nanoparticles. The carrier surface was modified and activated before enzyme immobilization using 3- aminopropyltriethoxysilane and glutaraldehyde. Testing of biocatalytic systems was carried out i

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2

Davis, Benjamin G. "SUGARS AND ENZYMES: PROTEIN GLYCOSYLATION AND GLYCOSIDE SYNTHESIS." In XXIst International Carbohydrate Symposium 2002. TheScientificWorld Ltd, 2002. http://dx.doi.org/10.1100/tsw.2002.411.

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3

Terwilliger, Thomas C., and Joel Berendzen. "Protein Crystallography: From X-ray diffraction spots to a three-dimensional image." In Signal Recovery and Synthesis. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/srs.1998.swa.1.

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Proteins are remarkable molecular machines that are essential for life. They can do many things ranging from the precise control of blood clotting to synthesizing complex organic compounds. Pictures of protein molecules are in high demand in biotechnology because they are important for applications such as drug discovery and for engineering enzymes for commercial use.

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4

Bogojevic, Oliver, Carl Arevang, and Zheng Guo. "Synthesis of complex phospholipid species." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/rlyh7861.

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Phospholipids are essential for the preservation of life on the planet and carry numerous critical roles and functions, including being the main constituents of the cell-membranes in both eukaryotic and prokaryotic cells, providing (more bioavailable) energy, and maintaining chemical and electrical processes in the body. The structural characteristics of phospholipids can vary greatly among species, however, commonly consist of a hydrophilic region (phosphate-containing head-group) and a hydrophobic region (fatty acids, €œtails€), providing the amphiphilic features and unique functions. The c

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5

Daineko, A. V., A. B. Bulatovski, and A. I. Zinchenko. "STUDY ON POTENTIAL ENGINEERING OF ESCHERICHIA COLI XANTHOSE PHOSPHORYLASE STRAIN-PRODUCER." In SAKHAROV READINGS 2021: ENVIRONMENTAL PROBLEMS OF THE XXI CENTURY. International Sakharov Environmental Institute of Belarusian State University, 2021. http://dx.doi.org/10.46646/sakh-2021-2-38-41.

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Genetic engineering is an actively developing branch of modern biotechnology. Using the methods of genetic engineering, it is possible to construct new forms of microorganisms that can synthesize a variety of substances, including enzymes. Xanthosine phosphorylase is the second purine nucleoside phosphorylase (PNP-II) in E. coli. This enzyme performs both reactions of phosphorolysis and the synthesis of purine deoxy / ribonucleosides. Due to this ability, xanthosine phosphorylase can catalyze the reaction of the formation of nicotinamide riboside. This substance is a precursor of the most impo

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6

Mazumder, Sonal, Suvojit Ghosh, Joseph O. Falkinham, and Ishwar K. Puri. "Factors Affecting the Assembly of Carbon Nanostructures With Cells and Enzymes." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13258.

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Carbon nanostructures were synthesized and deposited through flame synthesis on stainless steel grids and foils, and on bare and ferrofluid-painted silicon wafers at different nonpremixed flame locations to produce hydrophobic surfaces. The hydrophobicity is characterized through the contact angle for water droplets placed on the surface. The surface morphology of the nanoparticles is obtained from high-resolution FESEM images. Following synthesis and deposition the adherence, activity, and stability of bacterial cells, antibodies, and enzymes on the carbon nanostructures can be studied.

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7

Gao, Junshan. "BmPCD and BmDHPR: Two BH4 synthesis enzymes are activated in theBombyx morimutantlem." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.110345.

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8

Hassan, Shady S., Gwilym A. Williams, and Amit K. Jaiswa. "Fungus-Mediated Synthesis of magnetic nanoparticles for immobilisation of Pectolytic and xylanolytic enzymes." In The 6th World Congress on New Technologies. Avestia Publishing, 2020. http://dx.doi.org/10.11159/icnfa20.134.

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9

Szczepaniak, Sylwia A., Jacek Jemielity, Joanna Zuberek, Joanna Kufel, and Edward Darżynkiewicz. "Synthesis of nonhydrolyzable cap analog substituted sepharose for affinity purification of decaping enzymes." In XIVth Symposium on Chemistry of Nucleic Acid Components. Prague: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2008. http://dx.doi.org/10.1135/css200810461.

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10

Radzi, S. Mat, M. N. Mohd Zulhilmi, H. Mohd Noor, and M. Mohd Rehan. "Preliminary study on the synthesis of kojic acid monooleate using dual enzymes system." In GREEN DESIGN AND MANUFACTURE: ADVANCED AND EMERGING APPLICATIONS: Proceedings of the 4th International Conference on Green Design and Manufacture 2018. Author(s), 2018. http://dx.doi.org/10.1063/1.5066873.

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Звіти організацій з теми "Enzymes Synthesis"

1

Granot, David, Scott Holaday, and Randy D. Allen. Enhancing Cotton Fiber Elongation and Cellulose Synthesis by Manipulating Fructokinase Activity. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7613878.bard.

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a. Objectives (a) Identification and characterization of the cotton fiber FRKs; (b) Generating transgenic cotton plants overproducing either substrate inhibited tomato FRK or tomato FRK without substrate inhibition; (c) Generating transgenic cotton plants with RNAi suppression of fiber expressed FRKs; (d) Generating Arabidopsis plants that over express FRK1, FRK2, or both genes, as additional means to assess the contribution of FRK to cellulose synthesis and biomass production. b. Background to the topic: Cellulose synthesis and fiber elongation are dependent on sugar metabolism. Previous resu

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Lurie, Susan, John Labavitch, Ruth Ben-Arie, and Ken Shackel. Woolliness in Peaches and Nectarines. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7570557.bard.

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The overall goal of the research was to understand the processes involved in the development of woolliness in peaches and nectarines. Four specific hypotheses were proposed and in the course of the research evidence was gathered t support two of them and to not support two others. The hypotheses and a summary of the evidence are outlined below. 1. That woolliness arises from an imbalance between the activities of the cell wall pectin degrading enzymes. Using 'Flavortop' nectarines and 'Hermoza' peaches as model systems, storage regimes were manipulated to induce or prevent woolliness. The expr

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Prusky, Dov, Noel Keen, and Rolf Christoffersen. Involvement of Epicatechin in the Regulation of Natural Resistance of Avocado Fruit against Postharvest Pathogens. United States Department of Agriculture, January 1997. http://dx.doi.org/10.32747/1997.7613028.bard.

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In this project it was found that the activation of the mechanism of resistance in avocado fruits to Colletotrichum gloeosporioides depends on the increase of the level of the preformed antifungal diene. This increase is regulated by the synthesis of the flavonoid epicatechin present in the fruit peel. Epicatechin is an inhibitor of the enzyme lipoxygenase whose activity catalyze the breakdown of the antifungal diene. Increase in epicatechin concentration inhibits the breakdown of the antifungal compound and since the compound is continuously synthesized, both combined processes result in the

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Amir, Rachel, David J. Oliver, Gad Galili, and Jacline V. Shanks. The Role of Cysteine Partitioning into Glutathione and Methionine Synthesis During Normal and Stress Conditions. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7699850.bard.

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The objective of this research is to study the nature of the competition for cysteine (Cys), the first organic sulfur-containing compound, between its two main metabolites, glutathione (GSH) and methionine (Met). GSH plays a central role in protecting plants during various stresses, while Met, an essential amino acid, regulates essential processes and metabolites in plant cells through its metabolite S-adenosyl-Met. Our results, which are based on flux analysis and measurements of Met- metabolites, show that the flux towards Met synthesis is high during non-stress conditions, however the flux

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Sukenik, Assaf, Paul Roessler, and John Ohlrogge. Biochemical and Physiological Regulation of Lipid Synthesis in Unicellular Algae with Special Emphasis on W-3 Very Long Chain Lipids. United States Department of Agriculture, January 1995. http://dx.doi.org/10.32747/1995.7604932.bard.

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Various unicellular algae produce omega-3 (w3) very-long-chain polyunsaturated fatty acids (VLC-PUFA), which are rarely found in higher plants. In this research and other studies from our laboratories, it has been demonstrated that the marine unicellular alga Nannochloropsis (Eustigmatophyceae) can be used as a reliable and high quality source for the w3 VLC-PUFA eicosapentaenoic acid (EPA). This alga is widely used in mariculture systems as the primary component of the artificial food chain in fish larvae production, mainly due to its high EPA content. Furthermore, w3 fatty acids are essentia

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Sharon, Amir, and Maor Bar-Peled. Identification of new glycan metabolic pathways in the fungal pathogen Botrytis cinerea and their role in fungus-plant interactions. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597916.bard.

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The involvement of glycans in microbial adherence, recognition and signaling is often a critical determinant of pathogenesis. Although the major glycan components of fungal cell walls have been identified there is limited information available on its ‘minor sugar components’ and how these change during different stages of fungal development. Our aim was to define the role of Rhacontaining-glycans in the gray mold disease caused by the necrotrophic fungus B. cinerea. The research was built on the discovery of two genes, Bcdhand bcer, that are involved in formation of UDP-KDG and UDP-Rha, two UD

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Stewart, John M., Karl Hahn, and Wieslaw A. Klis. Synthetic Helizyme Enzymes. Fort Belvoir, VA: Defense Technical Information Center, August 1989. http://dx.doi.org/10.21236/ada211789.

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Manulis, Shulamit, Christine D. Smart, Isaac Barash, Guido Sessa, and Harvey C. Hoch. Molecular Interactions of Clavibacter michiganensis subsp. michiganensis with Tomato. United States Department of Agriculture, January 2011. http://dx.doi.org/10.32747/2011.7697113.bard.

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Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial wilt and canker of tomato, is the most destructive bacterial disease of tomato causing substantial economic losses in Israel, the U.S.A. and worldwide. The molecular strategies that allow Cmm, a Gram-positive bacterium, to develop a successful infection in tomato plants are largely unknown. The goal of the project was to elucidate the molecular interactions between Cmmand tomato. The first objective was to analyze gene expression profiles of susceptible tomato plants infected with pathogenic and endophytic Cmms

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Nicholson, Ralph, Reuven Reuveni, and Moshe Shimoni. Biochemical Markers for Disease Resistance in Corn. United States Department of Agriculture, May 1996. http://dx.doi.org/10.32747/1996.7613037.bard.

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The objective was to screen maize lines for their ability to express resistance based on biochemical traits. Cultivars were screened for retention of the hydroxamic acid DIMBOA and the synthesis of phenols (based on anthocyanin production) as markers for resistance. Lines were selected and inoculated with fungal pathogens (Exserohilum turcicum, Puccinia sorghi, Cochliobolus heterostraphus, Colletotricum graminicola.), and the Maize Dwarf Mosaic and Johnson Grass Mosaic viruses. Lines were screened in the field and greenhouse. Results showed that lines selected for augmented phenol synthesis do

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Gantt, Elisabeth, Avigad Vonshak, Sammy Boussiba, Zvi Cohen, and Amos Richmond. Carotenoid-Rich Algal Biomass for Aquaculture: Astaxanthin Production by Haematococcus Pluvialis. United States Department of Agriculture, August 1996. http://dx.doi.org/10.32747/1996.7613036.bard.

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The synthesis of carotenoids has been studied toward enhancing the production of ketocarotenoids, since fish and crustaceans raised by aquaculture require astaxanthin and other ketocaroteinoids in their feed for desirable pigmentation. Notable progress has been made in attaining the goals of determining improved conditions for ketocarotenoid production in Haematococcus pluvialis and in elucidating the carotenoid biosynthetic pathway. For production of astaxanthin a number of strains of the green alga Haematococcus were evaluated, a strain CCAG was found to be optimal for photoautotrophic growt

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