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Дисертації з теми "Escherichia coli Genetics"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 6 вересня 2023

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1

Baldwin, Thomas John. "Disease mechanism of enteropathogenic Escherichia coli." Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/34445.

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Colonization of gut mucosal surfaces by enteropathogenic Escherichia coli (EPEC) elicits a severe persistant diarrhoea in infants and young children without elaboration of enterotoxins or tissue invasion. The formation of a distinct ultrastructural lesion involving intimate adherence to cell surfaces, loss of microvilli and membrane perturbations, however, has for some time been recognized as an important component of pathogenesis, but the processes involved in lesion formation and its relevance to the disease state remained obscure. In this study intimate adherence of EPEC to surfaces of cult

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2

Lim, C. K. "Cloning of Streptomyces genes in Escherichia coli." Thesis, University of Leeds, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373850.

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3

Chua, K.-L. "Plasmid recombination in Escherichia coli K-12." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383777.

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4

Morgan, Brian Alexander. "The regulation of rpoBC in Escherichia coli." Thesis, University of Edinburgh, 1986. http://hdl.handle.net/1842/15431.

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5

Agostinho, Juliana Maria Avanci [UNESP]. "Diversidade genética, fatores de virulência e perfis de susceptibilidade a antimicrobianos de isolados de Escherichia coli provenientes do útero, da boca e das fezes de cadelas com piometra." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/108413.

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Made available in DSpace on 2014-08-13T14:50:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-07-26Bitstream added on 2014-08-13T18:01:12Z : No. of bitstreams: 1 000735330_20140826.pdf: 26004 bytes, checksum: 80491d16dd561bc066d6dacbdc900451 (MD5)<br>A piometra canina é uma enfermidade caracterizada pela inflamação do útero com acúmulo de exsudatos, acometendo principalmente fêmeas adultas. Ocorre na fase lútea do ciclo estral em decorrência de alterações hormonais e infecção bacteriana. É reconhecida como uma das principais causas de morte em cadelas e a Escherichia coli é o prin

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6

Sendy, Bandar. "Studies on transcription in Escherichia coli." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7576/.

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The expression of genes is tightly controlled, predominantly at the point of transcription. RNA polymerase (RNAP) must first bind to a deoxyribonucleic acid (DNA) promoter upstream of a gene to transcribe it. However, the ability of RNAP binding is dictated by the core promoter DNA sequence, the presence of transcription activator or repressor proteins and numerous other factors. The strength of promoters has been indirectly measured. Only a few studies have attempted to directly address the RNAP flux through transcription units, and further studies are still required. In the current study, th

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7

Ennis, Don Gregory. "Genetics of SOS mutagenesis." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184602.

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Previous genetic evidence suggested that RecA was required in SOS mutagenesis for its regulatory role and perhaps some other nonregulatory role (Mount, 1977; Blanco et al., 1982). I undertook a genetic study which confirmed the above studies and provided further evidence that RecA protein appeared to have a dual "role in mutagenesis; first, the cleavage of LexA repressor for the derepression of specific SOS genes and second, one or more additional role(s). For these studies a new phage mutagenesis assay was developed which allows rapid scoring of SOS mutagenesis in a large number of host mutan

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8

Woodgate, R. "A mechanism for UV mutagenesis in Escherichia coli." Thesis, University of Sussex, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375858.

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9

Hallewell, Jennyka, and University of Lethbridge Faculty of Arts and Science. "Shiga toxin-producing bacteriophage in Escherichia coli O157:H7." Thesis, Lethbridge, Alta. : University of Lethbridge, Deptartment of Biochemistry, 2008, 2008. http://hdl.handle.net/10133/776.

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Shiga toxin-producing E. coli (STEC) including E. coli O157:H7 are potential food and water borne zoonotic bacterial pathogens capable of causing outbreaks of severe illness in humans. The virulence of E. coli O157:H7 strains may be related to the type of Stx produced and several Stx2 variants have been identified which appear to differ in their ability to cause disease. Two lineages exist within O157 strains where lineage I is associated mainly with human and bovine isolates and lineage II is associated mainly with bovine isolates. The goal of this study was to identify and characterize a lin

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10

Miao, Yuanying, and 缪元颖. "The localization of E. coli persistent gene products." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45554791.

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11

Ho, Joanne Ming-Li. "Towards Sense Codon Reassignment in Escherichia Coli." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:26718706.

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Expansion of the genetic code through engineering of the translation machinery has vastly increased the chemical repertoire of the proteome. Incorporation of non-canonical amino acids (ncAAs) entails use of engineered aminoacyl-tRNA synthetase (AARS)•transfer RNA (tRNA) pairs that do not interact with the canonical aminoacylation machinery. Although engineered AARSs are selected for their ability to utilize ncAAs and discriminate against the twenty canonical amino acids, many utilize other ncAAs – a phenomenon known as polyspecificity. Specific incorporation of multiple ncAAs in a single polyp

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12

Picksley, S. M. "Inducible recombination and DNA repair in Escherichia coli K12." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332729.

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13

Taylor, L. "The recB and recC gene products of Escherichia coli." Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355080.

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14

Dave, Emma. "The physiology of the Escherichia coli pyruvate dehydrogenase complex." Thesis, University of Sheffield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364242.

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15

Doyle, Noel James. "Plasmid mediated error prone DNA repair in Escherichia coli." Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262031.

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16

Pickett, M. A. "Regulatory aspects of the gua operon of Escherichia coli." Thesis, University of Southampton, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381272.

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17

Malloch, Richard Anthony. "Ribonuclease III processing of Escherichia coli rpoBC messenger RNA." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/15259.

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18

Stumpf, Jeffrey D. "Polyphosphate kinase regulates DNA polymerase IV in Escherichia coli." [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3253637.

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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2007.<br>Title from PDF t.p. (viewed Nov. 19, 2008). Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0748. Adviser: Patricia L. Foster.

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19

Cowan, Peter J. "Controlled production of tryptophan by genetically-manipulated strains of Escherichia coli." Connect to thesis, 1992. http://repository.unimelb.edu.au/10187/1020.

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The tryptophan productivity of the genetically-manipulated strain JP4153 was increased 2.5-fold by introducing pMU78, a medium copy-number plasmid carrying a feedback-resistant trp operon. JP4153(pMU78) produced 23.5 g/l of tryptophan at a rate of 0.7 g/l/h when grown at 37 degrees C in a defined glucose and ammonium salts medium in a bench-scale fermentor.<br>During prolonged cultivation in the presence of antibiotic, the recombinant strain generated faster-growing, production-defective variants, which harboure mutated derivatives of pMU78. Insertion sequences were responsible for the two pre

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20

Park, Simon Fearon. "The biochemistry and genetics of osmoregulation in Escherichia coli." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254420.

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21

Xiao, Minfeng, and 肖敏鳳. "The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/206531.

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ompW encodes a widespread outer-membrane porin protein in Gram-negative bacteria. It has been implicated in bacterial responses to various antibiotics and environmental substances such as antibiotics, drugs and mouse mucus etc. Little is known, however, about its regulation and physiological roles during bacterial stress responses. Recently, comparative genomics studies revealed that the ompW gene is a core regulon of the global transcription factor FNR (Fumarate Nitrate Reduction) which mediates the transition from aerobic to anaerobic lifestyle of facultative bacteria. Anaerobiosis represent

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22

Huen, Shing-yan Michael, and 禤承恩. "A mechanistic study of lambdaphage-mediated recombination in E. coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B35321854.

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23

Sule, Preeti. "Phenotypic and Genotypic Effects of FlhC Mediated Gene Regulation in Escherichia Coli O157:H7." Diss., North Dakota State University, 2011. https://hdl.handle.net/10365/29205.

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Escherichia coli (E.coli) 0157:H7, a pathogen belonging to the enterohemorrhagic group of E.coli, has long been a concern to human health. The pathogen causes a myriad of symptoms in humans, ranging from diarrhea and malaise to renal failure. Human infection with the spread of the pathogen is mainly attributed to consumption of contaminated food material such as meat. Decontamination of meat via sprays have to date been the most commonly practiced method to reduce contamination, which now has little relevance in the face of developing resistance by the pathogen. In the following study we inves

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24

Bendezu, Felipe Oseas. "CELL SHAPE DETERMINATION IN ESCHERICHIA COLI." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1215459479.

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25

Leong, Mei-kid, and 梁美潔. "Development of an effective method to tag escherichia coli chromosomalgenes by recombineering." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30430811.

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26

黃雅誼 and Nga-yi Queenie Wong. "DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226851.

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27

Paton, Adrienne Webster. "Molecular characterization of variant shiga-like toxin genes of Escherichia coli /." Title page, contents and abstract only, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09php3118.pdf.

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28

Crickmore, Neil. "Analysis of a cell division gene cluster in "Escherichia coli"." Thesis, University of Warwick, 1987. http://wrap.warwick.ac.uk/2468/.

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Several genes, essential for cell growth and division in Escherichia coli, have been mapped to the 76 minute region of the chromosome. DNA sequencing of part of this region revealed three cell division genes (ftsY, ftsE, and ftsX) in a putative operon. A fourth gene (orf4) was also identified that was transcribed in the opposite direction to the putative operon. The genes rpof, f am, dnaM and ftsS have also been mapped to this region, but their location, relative to the putative operon, was unknown. In this study the fam and rpoU genes were independently cloned and shown to be allelic. The dna

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29

Anton, I. A. "Studies on the shikimate dehydrogenase gene of Escherichia coli." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234808.

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30

Stanley, Peter. "Studies on the mutator gene mutS of Escherichia coli K-12." Thesis, University of Kent, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328271.

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31

Ralph, Edward T. "Molecular characterisation of FNR, the anaerobic transcription regulator of Escherichia coli." Thesis, University of Sheffield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323194.

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32

Musuri, Periasamy Vigneshbabu. "Co-ordination of replication initiation with transcriptional regulation in Escherichia coli." Thesis, State University of New York at Buffalo, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10013540.

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<p>In Escherichia coli, replication initiates when the DnaA-ATP protein assembles at the origin oriC. Hda protein in complex with the beta sliding clamp protein (? clamp) on DNA, functions in altering the nucleotide bound form of the replication initiator DnaA protein from DnaA-ATP to DnaA-ADP in a process termed as Regulatory Inactivation of DnaA (RIDA). DnaA also functions as a transcription factor of several genes including the aerobic ribonucleotide reductase nrdAB genes. In this study, I have exploited the cold sensitive growth phenotype due to loss of Hda function and its suppressors to

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33

Dorrell, Nicholas. "Characterisation of a gene for photorepair in Escherichia coli K-12." Thesis, University of Bath, 1993. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357241.

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34

Agostinho, Juliana Maria Avanci. "Diversidade genética, fatores de virulência e perfis de susceptibilidade a antimicrobianos de isolados de Escherichia coli provenientes do útero, da boca e das fezes de cadelas com piometra /." Jaboticabal, 2013. http://hdl.handle.net/11449/108413.

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Orientador: José Moacir Marin<br>Banca: Janete Apparecida Desidério<br>Banca: Maria de Fátima Martins<br>Resumo: A piometra canina é uma enfermidade caracterizada pela inflamação do útero com acúmulo de exsudatos, acometendo principalmente fêmeas adultas. Ocorre na fase lútea do ciclo estral em decorrência de alterações hormonais e infecção bacteriana. É reconhecida como uma das principais causas de morte em cadelas e a Escherichia coli é o principal patógeno associado a esta doença. O objetivo deste estudo foi isolar e identificar cepas de E. coli provenientes de conteúdo intra uterino, boca

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35

Leung, Hang-mei Polly, and 梁杏媚. "Epidemiology and molecular genetics of verocytotoxigenic escherichia coli in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31245663.

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36

Kempsell, K. "The regulation of glutamate and melibiose utilisation in Escherichia coli." Thesis, University of Aberdeen, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384465.

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Wild type cells of <i>Escherichia coli</i> K-12 are unable to grow on glutamate as the sole source of carbon. Glutamate-utilising mutants have been isolated previously and found to exhibit enhanced glutamate transport. Mutations at two loci <i>gltS</i> and <i>gltR</i> and possibly a third <i>gltC</i> were previously shown by other workers to mediate enhanced glutamate permeability. The <i>gltR</i> locus was suggested to be a negative regulatory for the <i>gltS</i> gene. A mutation at the <i>gltS</i> locus, the <i>gltSo</i> mutation increases the activity of a Na<sup>+</sup>-stimulated glutamat

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37

Blakely, Garry William. "The regulation and stability of insertion sequence IS1 in Escherichia coli K12." Thesis, Queen's University Belfast, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335291.

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38

Walters, Robin George. "The organisation, expression and function of the UVRC gene of Escherichia coli." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237558.

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39

McFarlane, Ramsay James. "Molecular mechanisms of recombinogenic recircularization of linear plasmid DNA in escherichia coli." Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384995.

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40

Moffat, K. G. "The molecular cloning of the mutT gene of Escherichia coli K-12." Thesis, Cranfield University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373991.

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41

Huen, Shing-yan Michael. "A mechanistic study of lambdaphage-mediated recombination in E. coli." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B35321854.

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42

Wendel, Brian Michael. "Completion of DNA Replication in Escherichia coli." PDXScholar, 2018. https://pdxscholar.library.pdx.edu/open_access_etds/4406.

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To maintain genomic integrity, all cells must accurately duplicate their genetic material in order to provide intact and complete copies to each daughter cell following cell division. Successful inheritance of chromosomal information without changing even a single nucleotide requires accurate and robust DNA replication. This requires that cells tightly control replication initiation from the origin(s), processive elongation of the replisome, and the completion of DNA replication by resolving convergent replication forks ensuring that each sequence is duplicated without alteration. Unlike initi

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43

Nelson, Adam Michael. "Genomic analysis of pathogen evolution virulence gene acquisition and genetic erosion in Escherichia coli /." Diss., Connect to online resource - MSU authorized users, 2008.

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44

Benson, Fiona Elizabeth. "Molecular cloning and analysis of the ruv gene of Escherichia coli K12." Thesis, University of Nottingham, 1988. http://eprints.nottingham.ac.uk/30593/.

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Mutations in the ruv gene of Escherichia coli K-12 result in an increased sensitivity to agents that damage DNA. Studies presented in this thesis demonstrate that the ruv gene product is required for conjugational recombination in certain genetic backgrounds. From this it was inferred that the role of the ruv gene product was in the recombination repair of daughter strand gaps and double strand breaks in damaged DNA. In addition, the ruv gene product is shown to be required for the efficient recovery of F' transconjugants in certain genetic backgrounds, suggesting that recombination between tr

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45

Luo, Xuebin. "Structural Analysis of the Genes Encoding the Oxalocrotonate Branch of the Pseudomonas putida TOL Plasmid pDKI meta-cleavage Pathway and the Expression of the xy1G Gene Product in Escherichia coli." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500659/.

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Three overlapping DNA fragments from the lower operon of Pseudomonas putida TOL plasmid pDK1, covering the xy1IH genes and downstream flanking region, were cloned into pUC19. They include a 2.8 kbp XhoI fragment, a 2.7 kbp PstI fragment and a 2.0 kbp EcoRI-HindIII fragment. They were subjected to DNA sequence analysis. The xy1I (4-oxalocrotonate decarboxylase) and xy1H (4-oxalocrotonate tautomerase) genes were found to possess coding regions of 792 and 189 nucleotides, respectively. A possible transcriptional terminator resembling E. coli rho-independent terminators was identified downstream o

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46

Baertlein, Dawn Marie August. "Identification of phosphate starvation inducible mineral phosphate solubilization genes in Escherichia coli." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184606.

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Under conditions of phosphate starvation Escherichia coli can solubilize mineral phosphates, such as dicalcium phosphate, to orthophosphate which is then available for uptake and cell growth processes. lac operon fusions were created using MudX phage, and mineral phosphate solubilization (Mps) mutants were identified by their inability to solubilize mineral phosphate on plate assays. Four of these mutants have been mapped on the E. coli chromosome via Hfr matings and are located at two distinct portions of the chromosome; between 23 and 50 minutes and between 60 and 90 minutes. One mutant in e

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47

Ossanna, Nina. "Isolation and characterization of SOS constitutive mutations in Escherichia coli." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184398.

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Early events occurring during induction of the SOS response in Escherichia coli are poorly understood. In order to understand the early steps in SOS induction more fully, we have isolated several mutations which constitutively express the SOS regulon. Using a Mud(Apᴿ,lac) fusion to the SOS regulated gene sulA, we isolated Lac⁺ colonies as mutants in which RecA protein is constitutively activated for repressor cleavage. The mutations map to four loci: dam, lig, uvrD and recA. The extent of constitutive SOS induction in these mutants varied greatly, indicating different levels or types of signal

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48

Lozano, Gabriela Cazonato. "Avaliação do papel de genes envolvidos na mobilização de poli-3-hidroxibutirato em linhagens recombinantes de Escherichia coli." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-17052014-093733/.

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O P3HB é um tipo de poliéster sintetizado por bactérias como reserva de carbono e energia, e mobilizado na escassez destes. Um estudo com mutantes de Burkholderia sacchari, indicou o envolvimento de PhaZa1 e LonA na mobilização de P3HB. Este estudo avaliou o papel de PhaZa1 e LonA neste processo. A complementação heteróloga dos mutantes de B. sacchari, a partir dos genes phaZa1 e lonA de Ralstonia eutropha, restabeleceu a capacidade de mobilização dessas cepas, confirmando o envolvimento de seus produtos no processo. A partir de cepas recombinantes de Escherichia coli, abrigando tanto os genes

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Whitby, Matthew Conway. "Molecular and biochemical analysis of the recR operon of Escherichia coli K-12." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335405.

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Hufton, Simon Evan. "A structure-function analysis of the Escherichia coli vitamin Bâ†1â†2 receptor." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317422.

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