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Добірка наукової літератури з теми "Extracellular dehydration of an organism"

Автор: Grafiati

Опубліковано: 28 травня 2022

Оновлено: 31 липня 2025

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Статті в журналах з теми "Extracellular dehydration of an organism"

Coble, Jeffrey P., Justin L. Grobe, Alan Kim Johnson, and Curt D. Sigmund. "Mechanisms of brain renin angiotensin system-induced drinking and blood pressure: importance of the subfornical organ." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 308, no. 4 (2015): R238—R249. http://dx.doi.org/10.1152/ajpregu.00486.2014.

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It is critical for cells to maintain a homeostatic balance of water and electrolytes because disturbances can disrupt cellular function, which can lead to profound effects on the physiology of an organism. Dehydration can be classified as either intra- or extracellular, and different mechanisms have developed to restore homeostasis in response to each. Whereas the renin-angiotensin system (RAS) is important for restoring homeostasis after dehydration, the pathways mediating the responses to intra- and extracellular dehydration may differ. Thirst responses mediated through the angiotensin type

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Prykhodko, Olha. "STRUCTURAL CHANGES OF ORGANS AND TISSUES UNDER CONDITIONS OF DEHYDRATION DISORDERS OF WATER-SALT EXCHANGE." Clinical anatomy and operative surgery 21, no. 4 (2022): 98–106. http://dx.doi.org/10.24061/1727-0847.21.4.2022.52.

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Most processes in the body take place with the participation of water. It is an indispensable component of living organisms. Any violations of the water- electrolyte balance have consequences for the structural organization of all organs and tissues. Studying changes in the structural organization of organs and tissues during dehydration is an urgent task of both theoretical and practical medicine. The aim was to analyze and generalize data from modern scientiﬁ c literature on structural changes in organs and tissues during dehydration. Water is involved in maintaining a normal body temperatur

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Huang, Ju, Fulvio Reggiori, and Daniel J. Klionsky. "The Transmembrane Domain of Acid Trehalase Mediates Ubiquitin-independent Multivesicular Body Pathway Sorting." Molecular Biology of the Cell 18, no. 7 (2007): 2511–24. http://dx.doi.org/10.1091/mbc.e06-11-0995.

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Trehalose serves as a storage source of carbon and plays important roles under various stress conditions. For example, in many organisms trehalose has a critical function in preserving membrane structure and fluidity during dehydration/rehydration. In the yeast Saccharomyces cerevisiae, trehalose accumulates in the cell when the nutrient supply is limited but is rapidly degraded when the supply of nutrients is renewed. Hydrolysis of trehalose in yeast depends on neutral trehalase and acid trehalase (Ath1). Ath1 resides and functions in the vacuole; however, it appears to catalyze the hydrolysi

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Pankonien, Ines, Margarida C. Quaresma, Cláudia S. Rodrigues, and Margarida D. Amaral. "CFTR, Cell Junctions and the Cytoskeleton." International Journal of Molecular Sciences 23, no. 5 (2022): 2688. http://dx.doi.org/10.3390/ijms23052688.

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The multi-organ disease cystic fibrosis (CF) is caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) protein, a cAMP regulated chloride (Cl−) and bicarbonate (HCO3−) ion channel expressed at the apical plasma membrane (PM) of epithelial cells. Reduced CFTR protein results in decreased Cl− secretion and excessive sodium reabsorption in epithelial cells, which consequently leads to epithelial dehydration and the accumulation of thick mucus within the affected organs, such as the lungs, pancreas, gastrointestinal (GI) tract, reproductive system and sweat glan

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Dunne, W. Michael. "Bacterial Adhesion: Seen Any Good Biofilms Lately?" Clinical Microbiology Reviews 15, no. 2 (2002): 155–66. http://dx.doi.org/10.1128/cmr.15.2.155-166.2002.

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SUMMARY The process of surface adhesion and biofilm development is a survival strategy employed by virtually all bacteria and refined over millions of years. This process is designed to anchor microorganisms in a nutritionally advantageous environment and to permit their escape to greener pastures when essential growth factors have been exhausted. Bacterial attachment to a surface can be divided into several distinct phases, including primary and reversible adhesion, secondary and irreversible adhesion, and biofilm formation. Each of these phases is ultimately controlled by the expression of o

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Jaikumpun, Pongsiri, Kasidid Ruksakiet, Balázs Stercz, et al. "Antibacterial Effects of Bicarbonate in Media Modified to Mimic Cystic Fibrosis Sputum." International Journal of Molecular Sciences 21, no. 22 (2020): 8614. http://dx.doi.org/10.3390/ijms21228614.

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Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene encoding an epithelial anion channel. In CF, Cl− and HCO3− hyposecretion, together with mucin hypersecretion, leads to airway dehydration and production of viscous mucus. This habitat is ideal for colonization by pathogenic bacteria. We have recently demonstrated that HCO3− inhibits the growth and biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus when tested in laboratory culture media. Using the same bacteria our aim was to investigate the effects of HCO3− in artificial sputum medium (ASM), whose

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Lozano-Iturbe, Víctor, Noelia Blanco-Agudín, Emma Vázquez-Espinosa, et al. "The Binding of Pseudomonas aeruginosa to Cystic Fibrosis Bronchial Epithelial Model Cells Alters the Composition of the Exosomes They Produce Compared to Healthy Control Cells." International Journal of Molecular Sciences 25, no. 2 (2024): 895. http://dx.doi.org/10.3390/ijms25020895.

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Cystic fibrosis (CF) is a genetic disease that causes dehydration of the surface of the airways, increasing lung infections, most frequently caused by Pseudomonas aeruginosa. Exosomes are nanovesicles released by cells that play an essential role in intercellular communication, although their role during bacterial infections is not well understood. In this article, we analyze the alterations in exosomes produced by healthy bronchial epithelial and cystic fibrosis cell lines caused by the interaction with P. aeruginosa. The proteomic study detected alterations in 30% of the species analyzed. In

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Nicolaidis, S., O. Galaverna, and C. H. Metzler. "Extracellular dehydration during pregnancy increases salt appetite of offspring." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 258, no. 1 (1990): R281—R283. http://dx.doi.org/10.1152/ajpregu.1990.258.1.r281.

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The propensity to prefer and to consume salty foods varies considerably from person to person, and excessive salt intake has been linked to a number of pathological conditions. Extracellular dehydration occurs in humans after vomiting or diarrhea and is commonly observed during pregnancy. Because the hormonal responses to extracellular dehydration are known to increase salt appetite, we tested the hypothesis that extracellular dehydration during pregnancy increases the propensity of offspring to consume salt. Pregnant rats were treated with polyethylene glycol, which is known to produce extrac

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Harrison, Harold E. "Dehydration in Infancy: Hospital Treatment." Pediatrics In Review 11, no. 5 (1989): 139–43. http://dx.doi.org/10.1542/pir.11.5.139.

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Dehydration of a degree severe enough to require intravenous replacement of water and electrolytes indicates (1) depletion of extracellular fluid sodium and water to such an extent that reduction of plasma volume results or (2) distortion of the composition of extracellular fluid with consequent disturbance of physiologic function. Such distortion may be marked hypernatremia, severe depletion of extracellular bicarbonate, or disturbances of potassium concentration resulting in either hyperkalemia or hypokalemia. In addition, hypocalcemia or hypomagnesemia may require specific replacement of th

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Tarasova, Z. G., O. K. Kirilochev, and G. R. Sagitova. "Case report of hypotonic dehydration with outcome in cerebral edema in a child." Meditsinskiy sovet = Medical Council, no. 1 (March 4, 2022): 264–67. http://dx.doi.org/10.21518/2079-701x-2022-16-1-264-267.

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Dehydration is one of the most common disorders of water and electrolyte metabolism in young children. The reasons leading to the lack of water in the child’s body are very diverse. In clinical practice, a correct assessment of the pathophysiological mechanisms in various types of dehydration is necessary, which will allow timely identification of changes in various organ systems and conduct rational rehydration therapy. Water losses in children occur in a certain sequence. First of all, the intravascular subsector of the extracellular sector is subjected to water losses (clinical manifestatio

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Більше джерел

Дисертації з теми "Extracellular dehydration of an organism"

Ogbodo, Amobi, and A. Yusupova. "Morphological changes of thyroid gland in conditions of extracellular dehydration of organism." Thesis, Sumy State University, 2016. http://essuir.sumdu.edu.ua/handle/123456789/44928.

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A thyroid gland has a high ability to morphofunctional reformation under the impact of exo- and endogenous factors. Presently, the most studied are: the influence on the thyroid of ionizing radiation, temperature rate, physical activity, traumatic stress, tobacco smoke, various hormones, xenobiotics, drugs. However, in the modern scientific literature, the morphological changes in conditions of water-salt metabolism dysfunction is highlighted insufficiently.

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Сікора, Володимир Віталійович, Владимир Витальевич Сикора, Volodymyr Vitaliiovych Sikora, Валентина Іванівна Бумейстер, Валентина Ивановна Бумейстер та Valentyna Ivanivna Bumeister. "Клітинний склад регенерату великогомілкової кістки в умовах позаклітинного зневоднення". Thesis, Видавництво СумДУ, 2009. http://essuir.sumdu.edu.ua/handle/123456789/6779.

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Бончев, Сергій Дмитрович, Сергей Дмитриевич Бончев, Serhii Dmytrovych Bonchev та В. І. Полякова. "Морфологічні особливості довгих кісток при загальному зневодненні організму". Thesis, Вид-во СумДУ, 2005. http://essuir.sumdu.edu.ua/handle/123456789/6656.

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Хоменко, Інна Вікторівна, Инна Викторовна Хоменко та Inna Viktorivna Khomenko. "Структурні зміни щитоподібної залози за умов загального зневоднення організму". Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/44903.

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Структурна організація щитоподібної залози є об'єктом значної уваги клініцистів і морфологів. Щитоподібна залоза реагує на вплив різних факторів, а саме метеорологічних, геологічних, екологічних, оскільки її структура та функції тісно пов'язані з надходженням йоду та інших мікроелементів. Зміни органу можуть бути пов'язані з порушенням його ауторегуляції, в тому числі й на тканинному рівні. На основі цих фактрів виникає потреба комплексного вивчення структурних компонентів щитоподібної залози в динаміці за умов різних ступенів дегідратації, або зневоднення.

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Ковчун, В. Ю. "Мікроструктурні зміни підшлункової залози за умов загального зневоднення організму". Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/44901.

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Приходько, Ольга Олексіївна, Ольга Алексеевна Приходько, Olha Oleksiivna Prykhodko та ін. "Вивчення кількості CD3+ клітин щурів при легкому ступені позаклітинної дегідратації". Thesis, Сумський державний університет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/64347.

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Вступ. Тимус являється первинним лімфоїдним органом імунної системи, у якому відбувається антигеннезалежна проліферація та диференціація субпопуляції Т-лімфоцитів, які потрапляють у кров і розносяться по всьому організму. Субпопуляції Т-лімфоцитів (CD3+) є дуже важливими у функціональному плані. CD3+ клітини є поверхневими маркерами, специфічними для усіх клітин субпопуляції Т-лімфоцитів. За функціями відноситься до сімейства білків, що формують комплекс мембранної передачі сигналу, пов'язаний з Т-клітинним рецептором. За останні роки накопичено багато даних, що свідчать про важливу роль цен

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Масленко, А. О. "Гістологічна характеристика поперечно-посмугованих м'язів щурів за умов впливу загального зневоднення". Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/44900.

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Скелетна м’язова тканина складає близько 40 % маси тіла та містить у внутрішньоклітинному та позаклітинному просторі значні об’єми води. Тому вплив загальної дегідратації, яка виникає як наслідок значних та нераціональних фізичних навантажень за спекотливих умов, викликає порушення компенсаторних та регенераторних механізмів. Результатом цього стала необхідність поглибленого вивчення гістологічної характеристики м’язової тканини для подальшого розроблення нових експериментально-теоретичних підходів до проблеми регенерації м’язів, оптимізації протікання запальних процесів, які передбачають з'я

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Гордієнко, Олена Володимирівна, Елена Владимировна Гордиенко, Olena Volodymyrivna Hordiienko та К. О. Гончарова. "Методи, що використовуються при дослідженні репаративного остеогенезу". Thesis, Сумський державний університет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/64322.

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З метою вивчення особливостей репаративного остеогенезу губчастих кісток скелета в умовах загального зневоднення організму проводилися дослідження на безпородних щурах-самцях, що перебували в стаціонарних умовах віварію. У даній статті будуть розглянуті основні методи досліджень: гістологічне дослідження ділянки дефекту, морфометрія гістологічних препаратів, растрова електронна мікроскопія (PIXE) з зондовим мікроаналізом поверхні кістки, хімічний аналіз.

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Tomeo, Rodrigo Anderson. "Participação dos receptores gabaérgicos, serotoninérgicos e adrenérgicos do núcleo do trato solitário na ingestão de água e sódio induzida por diferentes tipos de desidratação." Universidade Federal de São Carlos, 2016. https://repositorio.ufscar.br/handle/ufscar/9038.

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Submitted by Ronildo Prado (ronisp@ufscar.br) on 2017-08-21T19:55:09Z No. of bitstreams: 1 TeseRAT.pdf: 1727135 bytes, checksum: b87fbc21ecbb31231523f49768c99fd8 (MD5)<br>Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-08-21T19:55:17Z (GMT) No. of bitstreams: 1 TeseRAT.pdf: 1727135 bytes, checksum: b87fbc21ecbb31231523f49768c99fd8 (MD5)<br>Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-08-21T19:55:23Z (GMT) No. of bitstreams: 1 TeseRAT.pdf: 1727135 bytes, checksum: b87fbc21ecbb31231523f49768c99fd8 (MD5)<br>Made available in DSpace on

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Книги з теми "Extracellular dehydration of an organism"

Clarke, Andrew. Freezing. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780199551668.003.0006.

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Freezing is a widespread ecological challenge, affecting organisms in over half the terrestrial environment as well as both polar seas. With very few exceptions, if a cell freezes internally, it dies. Polar teleost fish in shallow waters avoid freezing by synthesising a range of protein or glycoprotein antifreezes. Terrestrial organisms are faced with a far greater thermal challenge, and exhibit a more complex array of responses. Unicellular organisms survive freezing temperatures by preventing ice nucleating within the cytosol, and tolerating the cellular dehydration and membrane disruption t

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Vincent, Julian. Biomimetic materials. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780199674923.003.0010.

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Biological materials present the conventional materials scientist with alternative ways of achieving durability, recyclability, and adaptability. Technical materials are commonly designed to resist the initiation of cracks; biological materials control disaster by initiating failure where it can be more closely controlled and the strain energy can be more easily absorbed, at the same time controlling shape so that stress concentrations are avoided in sensitive areas. Most materials are hydrated and soft, achieving stiffness by dehydration and mineralization. The low energy of the predominant h

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Частини книг з теми "Extracellular dehydration of an organism"

Hahn, Robert G. "Fluid Physiology Part 2: Regulation of Body Fluids and the Distribution of Infusion Fluids." In Rational Use of Intravenous Fluids in Critically Ill Patients. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-42205-8_3.

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AbstractThe regulation of body fluids and infusion fluid distribution is a critical aspect of intensive care management. In this chapter, we explore the various control systems that maintain fluid balance in the body, including the kidneys, nervous system, and hormones. We also discuss the impact of disease and medical treatments on these control systems and the resulting fluid derangements, such as hypovolemia, volume depletion, and dehydration. The use of infusion fluids to distribute into the different body fluid spaces is analyzed, including the plasma, extracellular fluid, and total body

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Svoren, Martin, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf. "Approaches to Determine Nuclear Shape in Cells During Migration Through Collagen Matrices." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_7.

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AbstractFibrillar collagen is an abundant extracellular matrix (ECM) component of interstitial tissues which supports the structure of many organs, including the skin and breast. Many different physiological processes, but also pathological processes such as metastatic cancer invasion, involve interstitial cell migration. Often, cell movement takes place through small ECM gaps and pores and depends upon the ability of the cell and its stiff nucleus to deform. Such nuclear deformation during cell migration may impact nuclear integrity, such as of chromatin or the nuclear envelope, and therefore

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Omujal, Francis, and Joseph Mukisa. "Graphite Plate for Pervaporation Wound Therapy." In Alternative Medicine - New Insights [Working Title]. IntechOpen, 2024. https://doi.org/10.5772/intechopen.1006368.

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Graphitized carbon black is one of the carbon adsorbent biomaterials claimed to treat venomous wounds, but its efficacy has been debate by scientists. We have endeavored to explain the principle of the black stone as a graphite plate in wound therapy. The graphite plate is prepared using cattle bones by pyrolysis and has the ability to create vapor-pressure difference and promoting toxin dehydration in the wound will experience a reduction in pressure. In the process, bacterial membrane disruption and a reduction in reactive oxygen species production around the wound occur. The permanent vapor

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"Extracellular Dehydration Thirst and Drinking." In Thirst and Body Fluid Regulation. Cambridge University Press, 2021. http://dx.doi.org/10.1017/9781108878166.006.

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Toman, David, and Benoit De Crombrugghe. "Generation of cosmid DNA libraries and screening for extracellular matrix genes." In Extracellular Matrix. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199632213.003.0016.

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Abstract The application of molecular biological techniques to study the structure, function, and transcriptional regulation of extracellular matrix genes has provided a wealth of knowledge at the DNA level. A nine base pair repeat encoding Gly-X-Y at the amino acid level has been implicated as the ancestor of the collagen molecule. This repeat has been assembled into a 54 base pair exon which has been duplicated and combined with other functional groups to generate at least part of the family of collagen genes. Individual members of the collagen family show tissue specificity and provide diff

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Hryntsova, Nataliia. "STATE OF RAT PINEAL GLAND STRUCTURAL COMPONENTS IN THE CONDITIONS OF DIFFERENT EXTRACELLULAR DEHYDATION TERMS." In Integration of traditional and innovation processes of development of modern science. Publishing House “Baltija Publishing”, 2020. http://dx.doi.org/10.30525/978-9934-26-021-6-30.

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The monograph presents a theoretical generalization and a new solution for the research target to study the state of the pineal gland structural components in sexually mature rats under different terms of extracellular dehydration. The experiment was performed on 24 white sexually mature male rats aged 5-6 months. The animals of the experimental group were simulated extracellular dehydration of mild and heavy severity. Animals were kept and manipulated in compliance with national and international bioethics standards. Different degrees of extracellular dehydration severity caused negative chan

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Sandell, Linda J. "Matrix Biology: Extracellular Matrix – Building Function Through Complexity." In Mimicking the Extracellular Matrix. The Royal Society of Chemistry, 2015. http://dx.doi.org/10.1039/9781839168956-00003.

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The extracellular matrix (ECM) is the extracellular component of a multicellular organism or tissue that provides structural and biochemical support to the surrounding cells. The ECM functions to control many critical parameters of tissue homeostasis and response such as proliferation, apoptosis, development and morphogenesis. Characteristic of ECM is a complex interaction of specific large and small molecules that function as a composite structure: these structures can vary in different parts of the extracellular environment from a pericellular localization (concentrated around the cell) to i

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Storrie, Brian. "Chapter 1 The lysosome: Its role in the biology of the cell and organism." In Cellular Organelles and the Extracellular Matrix. Elsevier, 1996. http://dx.doi.org/10.1016/s1569-2582(05)80032-7.

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Lorca, Cristina, María Fernández-Rhodes, Jose Antonio Sánchez Milán, et al. "BP-EVs: A Novel Source of EVs in the Nanocarrier Field." In Extracellular Vesicles - Applications and Therapeutic Potential [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.113891.

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Extracellular vesicles (EVs) represent a complex mechanism of molecular exchange that has garnered significant attention in recent times. Nonetheless, identifying sustainable sources of biologically safe EVs remains challenging. This chapter delves into the utilization of fermented food industry by-products as a circular and secure reservoir of biocompatible EVs, dubbed as BP-EVs. BP-EVs demonstrate excellent oral bioavailability and biodistribution, with negligible cytotoxicity, and a preferential targeting capacity toward the central nervous system, liver, and skeletal tissues. The ease of e

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Sampaio, Joana, Joana Ferreira, Ana Carolina Santos, Manuel Bicho, and Maria Clara Bicho. "The Importance of the Extracellular Matrix in HPV-Associated Diseases." In Cervical Cancer - A Global Public Health Treatise. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99907.

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The extracellular matrix (ECM) is the non-cellular component of the tissues of our organism. It is the dynamic element that maintains a biochemical structure capable of supporting the organization and architecture of the tissue constituents. The diversity of ECM’s constituents gives it the biochemical and biophysical properties necessary to regulate its behavior and differentiation. ECM has an important role in the biology of cancer cell development and progression. Human papillomavirus infection (HPV) is the principal etiological agent of the most common sexually transmitted diseases. It is a

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Тези доповідей конференцій з теми "Extracellular dehydration of an organism"

Yoshimori, Takashi, Masaki Fukagawa, and Hiroshi Takamatsu. "Effect of Cell-to-Surface Interaction on Freeze Tolerance and Osmotic Response of Cells." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192404.

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Cryopreservation of tissues and organs, including artificial organs, could be one of the important steps in the medical service that brings the progress in the tissue engineering to realization. In this case, high viability of cryopreserved cells is critical to recovery after transplantation. In contrast, in the cryosurgery, which is expected to expand its application as a minimally invasive treatment of cancer, malignant cells should be destructed completely to prevent from recurrence. The appropriate freeze-thaw protocol is therefore needed to be established for cryopreservation or cryosurge

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Shefer, A. A., L. V. Yanshole, A. E. Grigorieva, and S. N. Tamkovich. "CHARACTERIZATION OF EXOSOMAL PROTEINS DERIVED FROM DIFFERENT BREAST CELL LINES AND SEARCH FOR CANDIDATE PROTEINS FOR LIQUID BIOPSY." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-395.

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Exosomes are extracellular vesicles secreted by the cells of the organism and are present in various biological fluids. They provide signal transduction between cells both in normal biological processes and in various pathological conditions including breast cancer. In this study, we isolated and characterized exosomes secreted by different breast cancer cell lines and identified proteins in their composition.

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Zhang, Aili, Lisa X. Xu, George A. Sandison, and Jiayao Zhang. "A Microscale Model for Prediction of Breast Cancer Cell Damage During Cryosurgery." In ASME 2003 Heat Transfer Summer Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/ht2003-47110.

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The morphology of most ductal carcinoma is characterized by tightly packed groups of small malignant cells. This special structure can make these breast cancer cells have different osmotic responses to freezing and affect the probability of damage from cellular dehydration and intracellular ice formation. A mathematical model has been developed to study the microscale damage of the breast cancer cells during cryosurgery by taking its unique structure into consideration. The model was built based on a spherical unit comprised of an extracellular region that surrounds several layers of cancer ce

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Sitaula, Ranjan, and Sankha Bhowmick. "Modeling of Osmotic Injury in Bovine Sperm During Desiccation." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19325.

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Although desiccation preservation offers promise as an alternative method for the preservation of mammalian cells, there has been limited success in achieving survival at very low water content [1]. Osmotic injury is one of the major damage factors during cellular dehydration. During the drying process, cells experience increased extracellular hypertonic environment as a result of evaporation of water. This factor coupled with the limited permeability of cell membranes leads to irreversible cellular damage. In the current study, we have studied the effect of hypertonic osmolality and exposure

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Finkbiner, J. J., K. L. Harrigan, K. C. Dee, and G. A. Livesay. "Fabrication and Properties of Collagen Fibers With Increased Surface Areas for Enhanced Fiber-Matrix Interactions." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176514.

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Collagen plays an important structural role in many natural tissues, such as ligaments and tendons. Due to its ubiquity in the human body and its commercial availability, biomaterials using collagen gel as a scaffold for an extracellular matrix are being developed as alternative treatments for soft tissue injuries [1]. The use of collagen fibers as a matrix in cell-seeded collagen gels has been shown to limit contraction of the gels as well as increase scaffold permeability and cell viability [2,3]. Additionally, it has been found that dehydration of collagen fibers increases fiber strength [4

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Bhaduri, S., S. K. Mitra, and A. Kumar. "Understanding Biofilm Growth Dynamics Within a Stagnant Culture of Sporosarcina Pasteurii." In ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-36778.

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Biofilms are bacterial colonies that form at interfaces, where bacteria are encased in extracellular polymeric substances (EPS). Biofilms are ubiquitous in both artificial systems and our environment. Here we focus on understanding biofilm growth within a stagnant pool of confined diluted culture of the bacteria. Sporosarcina pasteurii is taken as the model bacterium for this study. The motivation behind the choice of this organism stems from the fact that S. Pasteurii has the unique ability to precipitate calcite inside the host media which has tremendous applications in reservoir and restora

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Spilimbergo, Sara, Alessandro Zambon, Giovanni Giobbe, et al. "Long-term maintenance of dried acellular matrices." In 21st International Drying Symposium. Universitat Politècnica València, 2018. http://dx.doi.org/10.4995/ids2018.2018.7844.

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Dried and sterile acellular esophageal matrix was obtained within a new drying process based on the use of supercritical carbon dioxide (SC-CO2). Experiments were performed coupling a conventional detergent enzymatic treatment with two different drying methods: (i) SC-CO2 drying alone; (ii) dehydration in ethanol and a subsequent SC-CO2 drying. Long term preservation was achieved for several months after drying, demonstrating the maintenance of extracellular matrix (ECM) structure, mechanical properties and biocompatibility within cell repopulation studies in vitro. Overall, the results highli

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Gupta, M. S., and S. B. Nicoll. "Lower Macromer Concentration Enhances Nucleus Pulposus-Like Differentiation and Functional Extracellular Matrix Assembly by Human Marrow-Derived Stromal Cells Encapsulated in Photocrosslinked Carboxymethylcellulose Hydrogels." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80697.

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Intervertebral disc (IVD) degeneration is associated with dehydration and loss of integrity of the nucleus pulposus (NP) region of the disc. The NP is a tissue comprised mainly of proteoglycans and type II collagen (COL II) [1]. Tissue engineering therapies could provide viable NP replacements as an alternative to current surgical procedures. Recently, photocrosslinked carboxymethylcellulose (CMC) hydrogels were shown to support cell viability and the assembly of functional extracellular matrix (ECM) by encapsulated human marrow-derived mesenchymal stromal cells (hMSCs) when cultured in serum-

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Taboas, Juan M., and Amy L. Lerner. "Biological Gradient Regulated Predictive Model of Long Bone Growth." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-0204.

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Abstract Morphogenesis is regulated by a complex interaction of factors that are intrinsic and extrinsic to the cells composing the organism (e.g. genetic programming, cytokine gradients, and applied mechanical/electrical forces). Mechanical stimulation has been shown to affect bone growth, but the interaction of mechanical forces with biological factors during the development of a cartilage anlage into adult form is not clear. During skeletal development, chondrocytes produce cartilage through differential cell growth, cell volume changes, and extracellular matrix secretion. Cartilage cells a

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Bolyukh, Vladimir F., Igor I. Katkov, Vsevolod Katkov, and Ilya Yakhnenko. "KrioBlastTM: A Hyper-Fast Cooling and Thawing Scalable Device for Vitrification of Stem and Other Cells in Large Volumes." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-85468.

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Kinetic (very rapid) vitrification (KVF) is a very promising approach in cryopreservation (CP) of biological materials as it is simple, avoids lethal intracellular ice formation (IIF) and minimizes damaging dehydration effects of extracellular crystallization. Moreover, achieving the ultra-high rates, which would prevent IIF during cooling and devitrification during resuscitation, and achieve KVF for practically any type of cells with one protocol of cooling and re-warming would be the “Holy Grail” of cell cryobiology [3]. However such hyperrapid rates currently require very small sample size

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Звіти організацій з теми "Extracellular dehydration of an organism"

Schaffer, Arthur A., and Jocelyn Rose. Understanding Cuticle Development in Tomato through the Study of Novel Germplasm with Malformed Cuticles. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7593401.bard.

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Plant cuticle development and metabolism are still poorly understood, partly due to the chemical complexity of the cuticular layer. The overall research objective was to broaden and deepen our understanding of tomato fruit cuticle development by analyzing novel germplasm with cuticular malformations and by studying the transcriptome and proteome of the fruit epidermal tissues, as strategies to overcome the challenges posed by the recalcitrance of the biological system. During the project we succeeded in identifying two genes with major impact on cuticle development. One of these encoded the fi

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Chaiyabutr, Narongsak, Chollada Buranakarl, Somchai Chanpongsang, and Prapa Loypetjra. Changes in water metabolism in relation to renal functions of Swamp buffaloes during short term exposed to the solar radiation. Chulalongkorn University, 1989. https://doi.org/10.58837/chula.res.1989.29.

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The effects of short term heat exposure on the changes of body fluid and renal function were studied in four heifer buffaloes weighing between 174-207 kg. The animals were individually exposed to the sun up to 8 h each day over period of ten days (nonshaded period). Before this, they were kept in normal ambient temperature (control period). Each animal was fed same diet throughout experimental periods. In short term heat exposure, nonshaded buffaloes increased body water turnover rate while the marked decreases in total body water and blood volume were noted. On the first five days, there was

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Rafaeli, Ada, and Russell Jurenka. Molecular Characterization of PBAN G-protein Coupled Receptors in Moth Pest Species: Design of Antagonists. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7593390.bard.

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The proposed research was directed at determining the activation/binding domains and gene regulation of the PBAN-R’s thereby providing information for the design and screening of potential PBAN-R-blockers and to indicate possible ways of preventing the process from proceeding to its completion. Our specific aims included: (1) The identification of the PBAN-R binding domain by a combination of: (a) in silico modeling studies for identifying specific amino-acid side chains that are likely to be involved in binding PBAN with the receptor and; (b) bioassays to verify the modeling studies using mut

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Rafaeli, Ada, Russell Jurenka, and Chris Sander. Molecular characterisation of PBAN-receptors: a basis for the development and screening of antagonists against Pheromone biosynthesis in moth pest species. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7695862.bard.

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The original objectives of the approved proposal included: (a) The determination of species- and tissue-specificity of the PBAN-R; (b) the elucidation of the role of juvenile hormone in gene regulation of the PBAN-R; (c) the identificationof the ligand binding domains in the PBAN-R and (d) the development of efficient screening assays in order to screen potential antagonists that will block the PBAN-R. Background to the topic: Moths constitute one of the major groups of pest insects in agriculture and their reproductive behavior is dependent on chemical communication. Sex-pheromone blends are

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