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Дисертації з теми "Genetic vectors"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 25 липня 2025

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1

Theodorides, Kosmas. "Genetic and systematic studies on Cicadellidae vectors." Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368187.

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2

Shareck, Julie. "Isolation and characterization of a cryptic plasmid from Lactobacillus plantarum." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84072.

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Lactic acid bacteria (LAB), a group of generally recognized as safe (GRAS) organisms that metabolize sugars into primarily lactic acid, have traditionally been used for the fermentation and preservation of various foods and beverages. There is increasing interest in the genetic manipulation of LAB to improve existing characteristics or introduce novel, industrially pertinent phenotypes. However, because these bacteria have food-related applications, their genetic modification requires the use of food-grade genetic engineering tools. LAB plasmids, self-replicating extrachromosomal DNA mo

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3

Robson, Julia. "The construction of an expression vector for the transformation of the grape chloroplast genome." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53621.

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Thesis (MSc)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: The genetic information of plants is found in the nucleus, the mitochondria, and the plastids. The DNA of plastids is comprised of multiple copies of a double-stranded, circular, prokaryoticallyderived genome of -150 kb. The genome equivalents of plastid organelles in higher plant cells are an attractive target for genetic engineering as high protein expression levels are readily obtained due to the high genome copy number per organelle. The resultant proteins are contained within the plastid organelle and the correspon

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4

Ghosh, Arkasubhra. "Rational design of split gene vectors to expand the packaging capacity of adeno-associated viral vectors." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4712.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "December 2007" Includes bibliographical references.

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5

Wood, David Rowe Ding Jiahuan. "Design, optimization, and evaluation of conditionally active gene therapy vectors." Waco, Tex. : Baylor University, 2008. http://hdl.handle.net/2104/5153.

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6

Mück-Häusl, Martin Andreas. "Genetic engineering of adenoviral vectors for improved therapeutic applications." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-138269.

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7

Wong, Tik-wun Lina. "Construction of an infectious PRRSV cDNA clone and its use as a vector for foreign gene expression." Click to view the E-thesis via HKUTO, 2010. http://sunzi.lib.hku.hk/hkuto/record/B44251841.

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8

Van, Eeden C. (Christiaan). "The construction of gene silencing transformation vectors for the introduction of multiple-virus resistance in grapevines." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53764.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Viruses are some of the most important pathogens of grapevines. There are no effective chemical treatments, and no grapevine- or other natural resistance genes have been discovered against grapevine infecting viruses. The primary method of grapevine virus control is prevention by biological indexing and molecular- and serological screening of rootstocks and scions before propagation. Due to the spread of grapevine viruses through insect vectors, and in the case of GRSPaV the absence of serological screening, these metho

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9

Warren, Ann. "Transposable genetic elements in the mosquito Aedes aegypti." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237672.

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10

Limberis, Maria. "A lentiviral gene transfer vector for the treatment of cystic fibrosis airway disease." Title page, synopsis and list of contents only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phl735.pdf.

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"16th September 2002." Accompanying CD contains 2 MPEG clips with accompanying text, and a copy in PDF format of: Recovery of airway cystic fibrosis transmembrane conductance regulator function in mice with cystic fibrosis after single-dose lentivirus-mediated gene transfer / M. Limberis ... [et al.], published in Human gene therapy vol. 13 (2002). Bibliography: leaves xxix-li. This thesis focuses on modulating the physical barriers of the airway epithelium with mild detergents, so as to enhance gene transfer by a HIV-1 based lentivirus vector in vivo. The efficiency of the gene transfer was

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11

Ahmed, Seemin Seher. "rAAV-Mediated Gene Transfer For Study of Pathological Mechanisms and Therapeutic Intervention in Canavan's Disease: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/749.

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Анотація:

Canavan’s Disease is a fatal Central Nervous System disorder caused by genetic defects in the enzyme – aspartoacylase and currently has no effective treatment options. We report additional phenotypes in a stringent preclinical aspartoacylase knockout mouse model. Using this model, we developed a gene therapy strategy with intravenous injections of the aspartoacylase gene packaged in recombinant adeno associated viruses (rAAVs). We first investigated the CNS gene transfer abilities of rAAV vectors that can cross the blood-brain-barrier in neonatal and adult mice and subsequently used different

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12

Ahmed, Seemin Seher. "rAAV-Mediated Gene Transfer For Study of Pathological Mechanisms and Therapeutic Intervention in Canavan's Disease: A Dissertation." eScholarship@UMMS, 2012. http://escholarship.umassmed.edu/gsbs_diss/749.

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Анотація:

Canavan’s Disease is a fatal Central Nervous System disorder caused by genetic defects in the enzyme – aspartoacylase and currently has no effective treatment options. We report additional phenotypes in a stringent preclinical aspartoacylase knockout mouse model. Using this model, we developed a gene therapy strategy with intravenous injections of the aspartoacylase gene packaged in recombinant adeno associated viruses (rAAVs). We first investigated the CNS gene transfer abilities of rAAV vectors that can cross the blood-brain-barrier in neonatal and adult mice and subsequently used different

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13

Landazuri, Natalia. "Enhanced gene transfer using polymer-complexed retrovirus vectors." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/20677.

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14

com, shawnseet@gmail, and Shawn Ginn Ming Seet. "Genome sequence of bacteriophage ÖAR29 : a basis for integrative plasmid vectors." Murdoch University, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20060615.135718.

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Анотація:

The initial aim of this project was to characterise the integrative recombination mechanism of bacteriophage ÖAR29 , to provide a better understanding for development of the shuttle plasmid pBA as a site-specific Bacteroides integration vector. RT-PCR showed that the previously identified ÖAR29 recombination genes, integrase (Int) and excisionase (Xis), were transcribed from pBA in E. coli SCS110, B. thetaiotaomicron AR29 and B. uniformis AR20. In silico derived amino acid sequences from both genes showed only very low levels of similarity to other known Int and Xis in GenBank. To improve unde

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15

Seet, Shawn Ginn Ming. "Genome sequence of bacteriophage €AR29 : a basis for integrative plasmid vectors /." Access via Murdoch University Digital Theses Project, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20060615.135718.

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16

Maliti, Deodatus Vincent. "Ecological and genetic determinants of malaria vectors feeding and resting behaviours." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/6933/.

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Анотація:

Ecological and genetic factors play a key role in determining the behaviour of mosquito vectors, which in turn influences malaria transmission and epidemiology. Malaria vector control strategies such as long lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) rely on the timing and location of mosquito vector feeding behaviour, and their choice of resting habitat (inside houses versus outside). In some settings where these control measures have been intensively used, the major malaria vectors have been reported to change their behaviour to bite more often outdoors and/or earli

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17

Fuller, Maria. "A gene transfer system derived from human immunodeficiency virus type 1 (HIV-1)." Title page, table of contents, list of abbreviations and epitome only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phf9669.pdf.

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18

Wong, Tik-wun Lina, and 黃荻媛. "Construction of an infectious PRRSV cDNA clone and its use as a vectorfor foreign gene expression." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44251841.

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19

Shi, Bu-Jun. "Expression and function of cucumoviral genomes." Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phs5546.pdf.

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Анотація:

Bibliography: leaves 104-130. The aim of this thesis is to characterise subgenomic RNAs of cucumoviruses and the functions of their encoding genes. Strains of cucumber mosaic virus (CMV) are classified into two major subgroups (I and II) on the basis of nucleotide sequence homology. The V strain of tomato aspermy virus (V-TAV) and a subgroup I CMV strain (WAII) are chosen to determine whether the 2b genes encoded by these viruses are expressed 'in vivo'. For further investigation of the 2b gene function, cDNA clones of three genomic RNAs of V-TAV are constructed. Using the infectious cDNA clon

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20

Jeong, Pyengsoo. "Construction of a Cloning Vector Based upon a Rhizobium Plasmid Origin of Replication and its Application to Genetic Engineering of Rhizobium Strains." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc278189/.

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Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia as symbiont bacteroids within nodules of leguminous plant roots. Here, resident Rhizobium plasmids were studied as possible sources of components for the construction of a cloning vector for Rhizobium species.

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21

Thirion, Christian. "Improving gene transfer into skeletal muscle through genetic retargeting of adenoviral vectors." Diss., [S.l.] : [s.n.], 2004. http://edoc.ub.uni-muenchen.de/archive/00006339.

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22

Choi, Kwang Shik. "Genetic differentiation and introgression in malaria vectors of the Anopheles gambiae complex." Thesis, University of Liverpool, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431729.

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23

Lotti, Francesco. "Transcriptional targeting of lentiviral vectors to the erythroblastic progeny of hematopoietic stem cells." Thesis, Open University, 2003. http://oro.open.ac.uk/54805/.

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Анотація:

Correction of blood genetic disorders requires permanent gene transfer into self renewing, hematopoietic stem cells (HSC), and regulation of transgene expression in specific cell lineages. HIV-derived lentiviral vectors are very effective in transducing rare, non-dividing stem cell populations without altering their long-term repopulation and differentiation capacity. We developed a strategy for transcriptional targeting' of lentiviral vectors based on replacing the viral LTR control elements with cell lineage specific, genomic control elements. An upstream enhancer (HS2) of the erythroidspeci

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24

Sandersfeld, Lindsay Marie Maury Wendy J. "Identification of ebola glycoprotein mutants that exhibit increased transduction efficiency." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/428.

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25

Wolgamot, Gregory M. "Mus dunni endogenous virus (MDEV) /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/6319.

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26

Choudhury, Sourav Roy. "Developing an Adeno-Associated Viral Vector (AAV) Toolbox for CNS Gene Therapy: A Dissertation." eScholarship@UMMS, 2016. https://escholarship.umassmed.edu/gsbs_diss/809.

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Анотація:

Neurological disorders – disorders of the brain, spine and associated nerves – are a leading contributor to global disease burden with a sizable economic cost. Adeno-associated viral (AAV) vectors have emerged as an effective platform for CNS gene therapy and have shown early promise in clinical trials. These trials involve direct infusion into brain parenchyma, an approach that may be suboptimal for treatment of neurodegenerative disorders, which often involve more than a single structure in the CNS. However, overall neuronal transduction efficiency of vectors derived from naturally occurring

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27

Choudhury, Sourav Roy. "Developing an Adeno-Associated Viral Vector (AAV) Toolbox for CNS Gene Therapy: A Dissertation." eScholarship@UMMS, 2001. http://escholarship.umassmed.edu/gsbs_diss/809.

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Анотація:

Neurological disorders – disorders of the brain, spine and associated nerves – are a leading contributor to global disease burden with a sizable economic cost. Adeno-associated viral (AAV) vectors have emerged as an effective platform for CNS gene therapy and have shown early promise in clinical trials. These trials involve direct infusion into brain parenchyma, an approach that may be suboptimal for treatment of neurodegenerative disorders, which often involve more than a single structure in the CNS. However, overall neuronal transduction efficiency of vectors derived from naturally occurring

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28

Patterson, Sonya Marie. "Development of a cell-specific targeting strategy for therapeutic gene delivery vectors." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364767.

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29

Samaan, L. Z. "Comparative studies using Agrobacterium spp. as vectors for genetic engineering of higher plants." Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373321.

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30

Addison, Christina Lynn. "Construction and characterization of adenoviral vectors expressing cytokines for cancer immunotherapy /." *McMaster only, 1997.

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31

Thomas, Rodney H. "Machine Learning for Exploring State Space Structure in Genetic Regulatory Networks." Diss., NSUWorks, 2018. https://nsuworks.nova.edu/gscis_etd/1053.

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Анотація:

Genetic regulatory networks (GRN) offer a useful model for clinical biology. Specifically, such networks capture interactions among genes, proteins, and other metabolic factors. Unfortunately, it is difficult to understand and predict the behavior of networks that are of realistic size and complexity. In this dissertation, behavior refers to the trajectory of a state, through a series of state transitions over time, to an attractor in the network. This project assumes asynchronous Boolean networks, implying that a state may transition to more than one attractor. The goal of this project is to

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32

He, Jin. "Lentiviral vectors mechanisms of transgene silencing and functional characterization of novel genes /." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0006628.

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Анотація:

Thesis (Ph.D.)--University of Florida, 2004.<br>Typescript. Title from title page of source document. Document formatted into pages; contains 143 pages. Includes Vita. Includes bibliographical references.

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33

Brandén, Lars J. "The development of synthetic gene delivery systems /." Stockholm, 2001.

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34

Bae, Insoo. "Construction of a hybrid vector which allows for temperature regulation of expression of cloned genes in cyanobacterium, Synechocystis 6803." Virtual Press, 1988. http://liblink.bsu.edu/uhtbin/catkey/544002.

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A hybrid vector, pBVl, has been constructed which is capable of regulating expression of cloned genes in both Escherichia coli and in cyanobacterium Synechocystis 6803. Vector pBVl contains the powerful rightward promoter of bacteriophage lambda to ensure that cloned genes are transcibed at a high level. In addition, pBV] also contains a gene, cI857, encoding the lambda temperature sensitive repressor protein.The CAT gene coding for chloramphenicol aceyltransferase (CmActase) was cloned into pBV1 downstream of the lambda regulatory features to make plasmid pTC1. The expression of the CAT gene

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35

Ndabambi, Nonkululeko. "Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&amp.

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The aim of this thesis was to produce DNA expression constructs and use them to investigate the feasibility of recombinantly expression proteins for future interaction studies between human RBBP6 and p53 and pRb proteins.

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36

梁頌偉 and Chung-wai Leung. "Novel gene transfer vector targeted high affinity IL-2 receptor bearing cell." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226280.

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37

Leung, Chung-wai. "Novel gene transfer vector targeted high affinity IL-2 receptor bearing cell /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25248698.

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38

Rosli, Rozita. "Characterization of psb O mutants from cyanobacterium synechococcus PCC 7942 and expression of the wild-type gene in escherichia coli." Virtual Press, 1994. http://liblink.bsu.edu/uhtbin/catkey/941569.

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Анотація:

The 33 kilodalton (kD) manganese stabilizing protein (MSP) is intimately associated with the photolysis of water to molecular oxygen. The two main purposes of this study were: 1) to analyze previously constructed MSP mutants and 2) to subclone, express, and purify the wild-type MSP in Escherichia coli in order to investigate the relationship between structure and function of this protein.Growth rates were compared between bacterial cells harboring only the vector, the vector plus the wild-type MSP gene, and the vector plus a mutant MSP gene. No significant differences were seen. This result im

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39

Voronin, Yegor A. "Investigation of initiation of reverse transcription in retroviruses using vectors with two primer-binding sites." Morgantown, W. Va. : [West Virginia University Libraries], 2003. http://etd.wvu.edu/templates/showETD.cfm?recnum=3136.

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40

Francis, Murray A. "Characterisation of DNA damage inducible responses and repair in human cells using recombinant adenovirus vectors /." *McMaster only, 2000.

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41

Kjos, Sonia Alane. "Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas." [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-1299.

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42

Ustyugov, Alexey. "Expression and function of the small heat shock protein Hsp27 during embryogenesis of zebrafish Danio rerio." Online access for everyone, 2007. http://www.dissertations.wsu.edu/Thesis/Fall2007/a_ustyugov_080307.pdf.

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43

Myhre, Susanna. "Genetic re-targeting and de-targeting of adenovirus type 5 in order to create vectors for gene therapy /." Göteborg : Department of Microbiology and Immunology, The Sahlgrenska Academy at Göteborg University, 2007. http://hdl.handle.net/2077/7498.

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44

Chan, Fu-lun, and 陳賦麟. "Effective DNA delivery mediated by pH responsive peptides." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48333335.

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Анотація:

Non-viral vectors have been used to deliver therapeutic genes to treat different diseases. There are a variety of non-viral vectors such as liposomes, cationic polymers and peptides. Among all, pH responsive peptides showed excellent DNA transfection efficiency in many types of cell. These peptides are capable of changing their structural conformation as pH decreases, adopting a disordered structure which can destabilize endosomal membrane and therefore enhancing the release of DNA from endosomes into cytosol. Traditional pH responsive histidine-rich peptides showed good DNA transfection eff

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45

Xu, Zhenhua, and 许振华. "Functional characterization of cell cycle-related kinase in glioblastoma and development of gene delivery system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47752658.

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Cell cycle-related kinase (CCRK) is a 42 KDa serine/threonine protein kinase homologous to Cdk1, 2 and 7. Previous work has shown that CCRK regulates cell cycle transition by phosphorylating Cdk2 and Rb. More importantly, it was found that CCRK was a candidate oncogene in both glioblastoma multiform (GBM) and human colorectal cancer. However, the mechanistic role of CCRK in tumorigenicity is still not completely understood. In the first part of this thesis, I found that casein kinas II beta (CKIIβ) was one of proteins that interact with CCRK using the high-throughput yeast-two-hybrid

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46

Liu, Zun Kearney Christopher Michel. "New viral vectors for the expression of antigens and antibodies in plants." Waco, Tex. : Baylor University, 2009. http://hdl.handle.net/2104/5341.

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47

Du, Preez Jacques. "The development and characterisation of grapevine virus-based expression vectors." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4003.

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Thesis (PhD (Genetics))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Grapevine (Vitis vinifera L.) is a very important agricultural commodity that needs to be protected. To achieve this several in vivo tools are needed for the study of this crop and the pathogens that infect it. Recently the grapevine genome has been sequenced and the next important step will be gene annotation and function using these in vivo tools. In this study the use of Grapevine virus A (GVA), genus Vitivirus, family Flexiviridae, as transient expression and VIGS vector for heterologous protein expression

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48

Keeler, Allison M. "Gene Therapy for Very Long Chain Acyl-coA Dehydrogenase Deficiency Using Adeno-Associated Virus Vectors: A Dissertation." eScholarship@UMMS, 2012. https://escholarship.umassmed.edu/gsbs_diss/632.

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Анотація:

Very long chain acyl-coA dehydrogenase (VLCAD) is the rate-limiting step in mitochondrial fatty acid oxidation. VLCAD deficient mice and patients’ clinical symptoms stem from not only an energy deficiency but also long-chain metabolite accumulations. VLCAD deficient mice were treated systemically with 1x10 12 vector genomes of rAAV9-VLCAD. Expression was detected in the liver, heart and muscle. Also substantial expression of VLCAD was noted in the brain, where it was expressed across different sections of the brain and in different cell types with different morphologies. Biochemical correction

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49

Mück-Häusl, Martin Andreas [Verfasser], and Roland [Akademischer Betreuer] Beckmann. "Genetic engineering of adenoviral vectors for improved therapeutic applications / Martin Andreas Mück-Häusl. Betreuer: Roland Beckmann." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2011. http://d-nb.info/1018847227/34.

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Roig-Merino, Alicia [Verfasser], and Richard [Akademischer Betreuer] Harbottle. "Genetic Modification of Stem Cells Utilizing S/MAR DNA Vectors / Alicia Roig-Merino ; Betreuer: Richard Harbottle." Heidelberg : Universitätsbibliothek Heidelberg, 2018. http://d-nb.info/1177149265/34.

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