Готові списки джерел за темами / Helicase Dependent Amplification (HDA)

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Добірка наукової літератури з теми "Helicase Dependent Amplification (HDA)"

Автор: Grafiati
Опубліковано: 3 червня 2025
Оновлено: 31 липня 2025

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Статті в журналах з теми "Helicase Dependent Amplification (HDA)"

1

Uphoff, Timothy S., Blake W. Buchan, Nathan A. Ledeboer, Paul A. Granato, Judy A. Daly, and Tara N. Marti. "Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture." Journal of Clinical Microbiology 54, no. 9 (2016): 2388–90. http://dx.doi.org/10.1128/jcm.01268-16.

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Анотація:
We compared group AStreptococcus(GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.
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2

Wu, Xinghai, Chanfa Chen, Xizhi Xiao, and Ming Jun Deng. "Development of Reverse Transcription Thermostable Helicase-Dependent DNA Amplification for the Detection of Tomato Spotted Wilt Virus." Journal of AOAC INTERNATIONAL 99, no. 6 (2016): 1596–99. http://dx.doi.org/10.5740/jaoacint.16-0132.

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Анотація:
Abstract A protocol for the reverse transcription-helicase-dependent amplification (RT–HDA) of isothermal DNA was developed for the detection of tomato spotted wilt virus (TSWV). Specific primers, which were based on the highly conserved region of the N gene sequence in TSWV, were used for the amplification of virus's RNA. The LOD of RT–HDA, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP), and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were conducted using 10-fold serial dilution of RNA eluates. TSWV sensitivity in RT–HDA and RT-LAMP was 4 pg RNA com
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3

Kankanit, Pisamayarom, and Chaumpluk Piyasak. "RAPID LISTERIA MONOCYTOGENES ASSAY BASED ON HELICASE DEPENDENT AMPLIFICATION (HDA) AND NUCLEIC ACID HYBRIDIZATION IN BLUE SILVER NANOPLATES." International Journal of Research - Granthaalayah 5, no. 10 (2017): 322–35. https://doi.org/10.5281/zenodo.1043427.

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Анотація:
Listeria monocytogenes, a foodborne pathogen, is considered as one of the major problems in food safety. With strong safety regulations, a monitoring measure is essential for protecting the health and safety of consumers. Thus, a reliable monitoring method is required. In this study, a rapid assay based on a combination of helicase dependent amplification (HDA) and DNA signal detection via nucleic acid hybridization in blue silver nanoplates (AgNPls) was established. The assay started directly after short term enrichment in terrific broth using cotton ball swapping technique on seafood surface
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4

Padzil, Faiz, Abdul Razak Mariatulqabtiah, Wen Siang Tan, et al. "Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research." Animals 12, no. 1 (2021): 76. http://dx.doi.org/10.3390/ani12010076.

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Анотація:
Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), quantitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged
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5

Pisamayarom, Kankanit, and Piyasak Chaumpluk. "RAPID LISTERIA MONOCYTOGENES ASSAY BASED ON HELICASE DEPENDENT AMPLIFICATION (HDA) AND NUCLEIC ACID HYBRIDIZATION IN BLUE SILVER NANOPLATES." International Journal of Research -GRANTHAALAYAH 5, no. 10 (2017): 322–35. http://dx.doi.org/10.29121/granthaalayah.v5.i10.2017.2308.

Повний текст джерела
Анотація:
Listeria monocytogenes, a foodborne pathogen, is considered as one of the major problems in food safety. With strong safety regulations, a monitoring measure is essential for protecting the health and safety of consumers. Thus, a reliable monitoring method is required. In this study, a rapid assay based on a combination of helicase dependent amplification (HDA) and DNA signal detection via nucleic acid hybridization in blue silver nanoplates (AgNPls) was established. The assay started directly after short term enrichment in terrific broth using cotton ball swapping technique on seafood surface
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6

Li, Dawei, Bei Lv, Hao Zhang, Jasmine Yiqin Lee, and Tianhu Li. "Disintegration of cruciform and G-quadruplex structures during the course of helicase-dependent amplification (HDA)." Bioorganic & Medicinal Chemistry Letters 25, no. 8 (2015): 1709–14. http://dx.doi.org/10.1016/j.bmcl.2015.02.070.

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7

Zasada, Aleksandra Anna, Ewa Mosiej, Marta Prygiel, et al. "Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay." Biomedicines 10, no. 9 (2022): 2329. http://dx.doi.org/10.3390/biomedicines10092329.

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Анотація:
Rapid and accurate detection and identification of pathogens in clinical samples is essential for all infection diseases. However, in the case of epidemics, it plays a key role not only in the implementation of effective therapy but also in limiting the spread of the epidemic. In this study, we present the application of two nucleic acid isothermal amplification methods—reverse transcription helicase dependent amplification (RT-HDA) and reverse transcription loop-mediated amplification (RT-LAMP)—combined with lateral flow assay as the tools for the rapid detection of SARS-CoV-2, the etiologica
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8

Chemisova, O. S., O. A. Tsyrulina, A. L. Trukhachev, and A. K. Noskov. "Comparative analysis of methods for isothermal amplification of nucleic acids." Journal of microbiology, epidemiology and immunobiology 99, no. 1 (2022): 126–38. http://dx.doi.org/10.36233/0372-9311-176.

Повний текст джерела
Анотація:
In this review, methods for isothermal amplification of nucleic acids are considered and analyzed, in particular, loop isothermal amplification of DNA and RNA (LAMP/RT-LAMP), helicase-dependent amplification (HDA) and recombinase polymerase amplification (RPA). The advantages and disadvantages of each of the techniques are described. The possibility of their application in the molecular diagnostics of infectious diseases is evaluated. A brief review of the literature on the use of LAMP, HDA, RPA in the diagnostics of viral, bacterial infections and diseases of protozoal etiology was conducted.
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9

Buddhachat, Kittisak. "LAMP assay is an auxiliary tool for rapid and sensitive pathogen diagnosis in veterinary areas." Veterinary Integrative Sciences 20, no. 2 (2020): 247–51. http://dx.doi.org/10.12982/vis.2022.019.

Повний текст джерела
Анотація:
Molecular technique for nucleic acid amplification has become more advanced, especially isothermal nucleic acid amplification which can amplify DNA or RNA molecules at constant temperatures. This allows us to be independent of sophisticated equipment such as thermal cyclers or realtime-PCR. The feasibility of adopting molecular techniques for pathogen detection at point-of-care are approaching practical use. At present, there are various isothermal nucleic acid amplification technologies, including rolling cycle amplification (RCA), helicase-dependent amplification (HDA), recombinase polymeras
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10

Faizul Rahman, S., Z. Nor Amalina, I. Asma, and I. Aziah. "Asymmetric helicase-dependent amplification combined with lateral flow assay (HDA-LFA) for the detection of S. Paratyphi A." Asian Pacific Journal of Tropical Disease 4, no. 3 (2014): 243. http://dx.doi.org/10.1016/s2222-1808(14)60551-x.

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Більше джерел

Дисертації з теми "Helicase Dependent Amplification (HDA)"

1

Civit, Pitarch Laia. "Development of electrochemical biosensors and solid-phase amplification methods for the detection of human papillomavirus genes." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/84033.

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Анотація:
Un diagnòstic ràpid, precís i fiable és crucial per a la identificació d'una malaltia, com el càncer, on la detecció precoç pot millorar els resultants del tractament. El càncer de coll uterí és el tercer més comunament diagnosticat i la quarta causa de mort per càncer en les dones. La infecció persistent de virus del papil•loma humà d'alt risc (VPH) és la principal causa de càncer de coll uterí. Els biosensors electroquímics han rebut una important atenció a causa de les seves característiques. La seva compatibilitat amb tecnologies de microfabricació els fa atractius per al diagnòstic d'ADN
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Частини книг з теми "Helicase Dependent Amplification (HDA)"

1

Ao, Wanyuan, and Robert Jenison. "Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-535-4_7.

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2

Manzoor, Subaya, Zahoor Ahmad Mir, Tanveer Ahmad Wani, et al. "RECENT ADVANCES IN DIAGNOSTIC APPROACHES FOR PLANT PATHOGEN DETECTION." In Futuristic Trends in Biotechnology Volume 3 Book 3. Iterative International Publisher, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bfbt3p1ch2.

Повний текст джерела
Анотація:
Emerging plant pathogens persist as a serious threat to the food security and stability of ecosystems, casting a looming shadow over the global economy. Plant pathogens afflicting one of the biggest threats to agriculture worldwide, capable of amplifying harvest losses by as much as 30 percent of the total global annual economic output, resulting in the depletion of food production worth billions of dollars. Therefore, it becomes important to precisely delineate the issue and explore solutions for addressing these ailments. Traditional methods are deemed insufficient in terms of precision, acc
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