Готові списки джерел за темами / High resolution melting analysi

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  2. Дисертації
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  5. Тези доповідей конференцій
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Добірка наукової літератури з теми "High resolution melting analysi"

Автор: Grafiati
Опубліковано: 10 березня 2023
Оновлено: 31 липня 2025

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Статті в журналах з теми "High resolution melting analysi"

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Carillo, Serge, Laurent Henry, Eric Lippert, et al. "Nested High-Resolution Melting Curve Analysis." Journal of Molecular Diagnostics 13, no. 3 (2011): 263–70. http://dx.doi.org/10.1016/j.jmoldx.2010.12.002.

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Erali, Maria, and Carl T. Wittwer. "High resolution melting analysis for gene scanning." Methods 50, no. 4 (2010): 250–61. http://dx.doi.org/10.1016/j.ymeth.2010.01.013.

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Tong, S. Y. C., and P. M. Giffard. "Microbiological Applications of High-Resolution Melting Analysis." Journal of Clinical Microbiology 50, no. 11 (2012): 3418–21. http://dx.doi.org/10.1128/jcm.01709-12.

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Ro, Na Young, On Sook Hur, Ho Cheol Ko, et al. "Evaluation of Resistance in Pepper Germplasm to Cucumber mosaic virus by High Resolution Melting Analysis." Research in Plant Disease 18, no. 4 (2012): 290–97. http://dx.doi.org/10.5423/rpd.2012.18.4.290.

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Zambounis, Antonios, Eleni Stefanidou, Panagiotis Madesis, Jovana Hrustić, Milica Mihajlović, and Brankica Tanović. "Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis." Plant Protection Science 57, No. 1 (2020): 38–46. http://dx.doi.org/10.17221/35/2020-pps.

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Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the three main causal agents of brown rot, which is one of the most important diseases of stone fruits in pre- and postharvest conditions. Nowadays, the need for the precise genotyping of these Monilinia species in terms of the genetic diversity of their populations or differences in their pathogenicity and host range is a prerequisite for any efficient disease management. In our study, the genetic structure of Monilinia populations in Serbia from three geographically distinct regions was investigated employing <br /> a hi
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Wittwer, Carl T., Gudrun H. Reed, Cameron N. Gundry, Joshua G. Vandersteen, and Robert J. Pryor. "High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen." Clinical Chemistry 49, no. 6 (2003): 853–60. http://dx.doi.org/10.1373/49.6.853.

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Abstract Background: High-resolution amplicon melting analysis was recently introduced as a closed-tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003;49:396–406). The technique required a fluorescently labeled primer and was limited to the detection of mutations residing in the melting domain of the labeled primer. Our aim was to develop a closed-tube system for genotyping and mutation scanning that did not require labeled oligonucleotides. Methods: We studied polymorphisms in the hydroxytryptamine receptor 2A (HTR2A) gene (T102C), β-globin (hemoglobins S and C) gen
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Simko, Ivan. "High-Resolution DNA Melting Analysis in Plant Research." Trends in Plant Science 21, no. 6 (2016): 528–37. http://dx.doi.org/10.1016/j.tplants.2016.01.004.

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Mader, Eduard, Joana Ruzicka, Corinna Schmiderer, and Johannes Novak. "Quantitative high-resolution melting analysis for detecting adulterations." Analytical Biochemistry 409, no. 1 (2011): 153–55. http://dx.doi.org/10.1016/j.ab.2010.10.009.

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Janavicius, Ramunas, Dovile Matiukaite, Arturas Jakubauskas, and Laimonas Griskevicius. "Microsatellite Instability Detection by High-Resolution Melting Analysis." Clinical Chemistry 56, no. 11 (2010): 1750–57. http://dx.doi.org/10.1373/clinchem.2010.150680.

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BACKGROUND Microsatellite instability (MSI) is an important marker for screening for hereditary nonpolyposis colorectal cancer (Lynch syndrome) as well as a prognostic and predictive marker for sporadic colorectal cancer (CRC). The mononucleotide microsatellite marker panel is a well-established and superior alternative to the traditional Bethesda MSI analysis panel, and does not require testing for corresponding normal DNA. The most common MSI detection techniques—fluorescent capillary electrophoresis and denaturing HPLC (DHPLC)—both have advantages and drawbacks. A new high-resolution meltin
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Wittwer, Carl T. "High-resolution DNA melting analysis: advancements and limitations." Human Mutation 30, no. 6 (2009): 857–59. http://dx.doi.org/10.1002/humu.20951.

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Дисертації з теми "High resolution melting analysi"

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Dempsey, Nunez Laura. "Spectrum of mutations in MMAA identified by high resolution melting analysis." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110535.

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Анотація:
The gene product of MMAA is required for the intracellular metabolism of cobalamin (Cbl). Mutations in this gene lead to the cblA class of disorders, characterized by isolated methylmalonic aciduria. We have been concerned that somatic cell methods of diagnosis may miss patients with mild cellular phenotypes. A high resolution melting (HRM) analysis assay was developed to rapidly scan the coding exons and flanking intronic regions of the MMAA genes for variants. DNA from 96 unaffected reference individuals, 72 patients with complementation confirmed cblA, and 181 patients with elevated isolate
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Illson, Margaret. "Spectrum of mutations in MMAB identified by high resolution melting analysis." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110564.

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Анотація:
Pathogenic variants in the MMAB gene (OMIM 607958) are responsible for the cblB class of cobalamin-responsive methylmalonic aciduria (MMA) (OMIM 251110). MMAB encodes cobalamin adenosyltransferase, a mitochondrial enzyme responsible for the formation of adenosylcobalamin (AdoCbl). AdoCbl subsequently functions as a cofactor for methylmalonyl-CoA mutase (MCM) during the isomerization of L-methylmalonyl-CoA to succinyl-CoA. Somatic cells studies have been used to evaluate patient samples for cobalamin related disorders. Due to high basal levels of propionate incorporation, some patients wi
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Souza, Roberto Antonio de. "Genotipagem de linhagens de Yersinia spp. por high-resolution melting analysis." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-27062013-151724/.

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O gênero Yersinia pertence à família Enterobacteriaceae e compreende 17 espécies. Y. pestis, Y. pseudotuberculosis e Y. enterocolitica são reconhecidamente patógenos de humanos e animais. Y. pestis cause a peste. Y. pseudotuberculosis e Y. enterocolitica são agentes causadores, sobretudo, de gastroenterites transmitidas por água e alimentos. As demais 14 espécies são, usualmente, consideradas não-patogênicas, com exceção de Y. ruckeri sorogrupo O:1 que causa infecções em peixes. Nas últimas décadas, a tipagem molecular tornou-se uma importante ferramenta nos estudos filogenéticos de numerosos
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Darbandy, Ashna. "Optimization of High Resolution Melting Analysis for Detection of KRAS Gene Mutations." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-130751.

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Анотація:
<p><strong>Background: </strong>Mutations of the KRAS oncogene occur in a variety types of human tumors. By assessing the mutation status of KRAS, clinicians can predict patient response to anti-EGFR therapy such as cetuximab (Erbitux®) or panitumumab (Vectibix®) in patients with metastatic colorectal cancer. The aim of this study was to optimize a real-time PCR method followed by high resolution melting analysis (HRM) in a single step for detection of most common mutations within the KRAS gene. <strong>Methods:</strong> Seven DNA samples with predefined KRAS mutations and 19 tumor samples fro
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Burrows, Adria Michelle. "A comparative ancestry analysis of Y-chromosome DNA haplogroups using high resolution melting." University of the Western Cape, 2018. http://hdl.handle.net/11394/6536.

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>Magister Scientiae - MSc<br>The objective of this study is to deduce paternal ancestry using ancestry informative single nucleotide polymorphisms (SNPs) by means of High Resolution Melting (HRM). This was completed by producing a multiplex system that was designed in a hierarchical manner according to the YSNP tree. This project mainly focused on African ancestry and was used to infer paternal ancestral lineages on the Johannesburg Coloured population. South Africa has a diverse population that has ancestral history from across the globe. The South African Coloured population is the mo
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Michelle, Burrows Adria. "A comparative ancestry analysis of Y-chromosome DNA haplogroups using high resolution melting." University of the Western Cape, 2018. http://hdl.handle.net/11394/6489.

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Magister Scientiae - MSc (Biotechnology)<br>The objective of this study is to deduce paternal ancestry using ancestry informative single nucleotide polymorphisms (SNPs) by means of High Resolution Melting (HRM). This was completed by producing a multiplex system that was designed in a hierarchical manner according to the YSNP tree. This project mainly focused on African ancestry and was used to infer paternal ancestral lineages on the Johannesburg Coloured population. South Africa has a diverse population that has ancestral history from across the globe. The South African Coloured population i
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PIMENTEL, ROMERO CESAR HUGO. ""INNOVATIVE SIGNAL PROCESSING TECHNIQUES IN BIOENGINEERING: COMPRESSED SENSING AND HIGH RESOLUTION DNA MELTING ANALYSIS"." Doctoral thesis, Università degli studi di Ferrara, 2021. http://hdl.handle.net/11392/2487913.

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The first part starts with an introduction of the Compressed Sensing (CS) theory. After that, an overview of the state-of-the art of some CS adaptations by using additional priors in the different stages is presented in order to explain the new CS adaptation proposed in this work. Some of the CS adaptations reviewed are confronted using synthetic signals, synthetic electrocardiograph (ECG) signals and electroencephalographic (EEG) signals. The second part provides general concepts of biology and current developments in bioinformatics to read and and analyze the DNA. Questions like what a seque
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Tsang, Ho-yin, and 曾皓言. "Detection of clinically silent beta-globin gene mutations in Chinese using high resolution melting analysis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48334182.

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Анотація:
Mutations in the beta-globin (β-globin) gene cause beta-thalassaemia (β-thalassaemia).The screening strategy for β-thalassaemiais based on the value of mean corpuscular volume (MCV) from the complete blood count (CBC) data. Current laboratory practice considers blood samples with MCV higher than 80fL as normal. No further assessment will be done on these samples. However, there are clinically silent β-globin gene mutations with MCV higher than 80fL, for example, heterozygous haemoglobin E (HbE). The importance of finding out this kind of mutations is due to the serious outcome when they occur
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Ho, Sophia KW, and 何廣慧. "Detection of clinically silent alpha-globin gene mutations in Chinese using high resolution melting analysis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206558.

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α-thalassemia is an inherited globin gene disorder commonly found among the Chinese population. It is composed of both non-deletional and deletional α-globin gene mutations. Classical α-thalassemia presents with red cell microcytosis but silent cases with a normal mean corpuscular volume (MCV) are also seen. Routine laboratory testing methods for large-scale detection of silent α-thalassemia mutations are onerous and time-consuming. Furthermore, methods such as denaturing high performance liquid chromatography (HPLC) or denaturing gradient gel electrophoresis (DGGE) for scanning of point muta
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Ozbak, Hani. "The application of High Resolution Melting Analysis (HRMA) for rapid detection of bacteria responsible for bloodstream infections." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/the-application-of-high-resolution-melting-analysis-hrma-for-rapid-detection-of-bacteria-responsible-for-bloodstream-infections(b3d5c15b-9541-44c2-873c-f7a32fc60282).html.

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Background: The diagnosis of bloodstream infection is a significant challenge for healthcare providers and is often associated with severe illness (sepsis) and poor outcomes. Rapid detection and identification of pathogens followed by characterisation of antibiotic resistance could help direct early treatment and improve patient care. Standard blood culture methods, which usually take 2-5 days to complete, can confirm if there is a bacteraemia or not in suspected patients. However, molecular approaches have been developed and are being increasingly investigated to overcome disadvantages of cul
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Книги з теми "High resolution melting analysi"

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Kaushik, Sanket, and Nagendra Singh, eds. Current Developments in the Detection and Control of Multi Drug Resistance. BENTHAM SCIENCE PUBLISHERS, 2022. http://dx.doi.org/10.2174/97898150498791220101.

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The rise in the incidence of infections is caused by multi drug resistant (MDR) bacteria, it is essential to elucidate the basic mechanism of antibiotic resistance to discover effective methods for diagnosis and treatment of infections. The use of pathogen-specific probes offers a faster alternative for pathogen detection and could improve the diagnosis of infection. High resolution melting analysis techniques are useful for the detection of multi drug resistant pathogens. Rational Structural Based Drug Design is a common method to identify a lead compound and take it forward for further devel
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Частини книг з теми "High resolution melting analysi"

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Tucker, Elise J., and Bao Lam Huynh. "Genotyping by High-Resolution Melting Analysis." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0446-4_5.

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Bruzzone, Carol M., and Clifford J. Steer. "High-Resolution Melting Analysis of Single Nucleotide Polymorphisms." In Molecular Typing of Blood Cell Antigens. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2690-9_2.

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Vossen, Rolf H. A. M. "Genotyping DNA Variants with High-Resolution Melting Analysis." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6442-0_2.

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Łukasik, Ewa, Kazimiera Waśniowska, Magdalena Grodecka, Edyta Majorczyk, and Marcin Czerwiński. "High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens." In Molecular Typing of Blood Cell Antigens. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2690-9_7.

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Grazina, Liliana, Joana Costa, Joana S. Amaral, and Isabel Mafra. "High-Resolution Melting Analysis as a Tool for Plant Species Authentication." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1201-9_5.

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Day, Robert, and Richard Macknight. "Screening for Imprinted Genes Using High-Resolution Melting Analysis of PCR Amplicons." In Methods in Molecular Biology. Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-773-0_5.

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Derzelle, Sylviane. "Single-Nucleotide Polymorphism Discrimination Using High-Resolution Melting Analysis for the Genotyping of Bacillus anthracis." In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2004-4_26.

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Ozkok, Fatma Ozge, and Mete Celik. "Classification of High Resolution Melting Curves Using Recurrence Quantification Analysis and Data Mining Algorithms." In Engineering Cyber-Physical Systems and Critical Infrastructures. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-09753-9_49.

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Ferro, Marta, Hada C. Macher, Pilar Noguerol, et al. "Non-invasive Prenatal Diagnosis of Feto-Maternal Platelet Incompatibility by Cold High Resolution Melting Analysis." In Advances in Experimental Medicine and Biology. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-42044-8_13.

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Torres, Anthony, Robert Givens, and Reginald Gaudino. "Haplotype and Presence–Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4642-7_11.

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Тези доповідей конференцій з теми "High resolution melting analysi"

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Bel’kovich, Y. I., E. V. Snitkov, and B. A. Tonkonogov. "ALGORITHMS FOR RESULTS’ CLUSTERING OF MELTING CURVES’ ANALYSIS WITH HIGH RESOLUTION." In SAKHAROV READINGS 2022: ENVIRONMENTAL PROBLEMS OF THE XXI CENTURY. International Sakharov Environmental Institute of Belarusian State University, 2022. http://dx.doi.org/10.46646/sakh-2022-2-416-419.

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Basic principles of data clustering are considered, stages of its implementation and methods to determine object similarity and result interpretation are described and characteristics of various conduct methods for DNA melting are given.
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Boulton, J., E. Masiero, and T. Sgamma. "Barcode High Resolution Melting (Bar-HRM) analysis for authentication of Echinacea products." In GA – 69th Annual Meeting 2021, Virtual conference. Georg Thieme Verlag, 2021. http://dx.doi.org/10.1055/s-0041-1736809.

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Daugaard, Iben L., Lasse S. Kristensen, Tina Kjeldsen, Stephen Hamilton Dutoit, Henrik Hager, and Lise Lotte Hansen. "Abstract 2115: Increased sensitivity ofKRASmutation detection by High-Resolution Melting analysis of COLD-PCR products." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-2115.

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Черноок, А. Г., М. С. Баженов, Е. А. Никитина, and В. В. Панченко. "SEARCH FOR MUTATIONS IN THE GRF2-2R GENE IN THE AREA OF THE miRNA396 BINDING SITE IN RYE BY HIGH-RESOLUTION DNA MELTING SEEDING METHOD." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2021. http://dx.doi.org/10.48397/arriab.2021.21.xxi.021.

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Анотація:
Быстрые темпы развития современной биотехнологии требуют использования новейших методов для получения результатов исследований. Высокочувствительный анализ плавления (High Resolution Melting, HRM-анализ) — это метод обнаружения полиморфизмов нуклеотидной последовательности ПЦР-ампликонов, основанный на наблюдении процесса денатурации (плавления) двуцепочечной ДНК при строго контролируемом постепенном нагревании. Наблюдение денатурации ДНК происходит с применением интеркалирующего красителя и детекции флуоресценции на приборе в реальном времени. The rapid pace of development of modern biotechno
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Hussein, Rusul Muzher, Alaa Shawqi Abdulbari, and Mohammed A. B. Al-Ayash. "Detection of hepatitis B virus for genotype B and C by using high resolution melting analysis." In PROCEEDING OF THE 1ST INTERNATIONAL CONFERENCE ON ADVANCED RESEARCH IN PURE AND APPLIED SCIENCE (ICARPAS2021): Third Annual Conference of Al-Muthanna University/College of Science. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0093406.

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Deschoolmeester, Vanessa, Christophe Deben, Marc Baay, et al. "Abstract 2103: High resolution melting analysis: a sensitive screening method for the detection of MDM2 promotor SNP309." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-2103.

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Deschoolmeester, Vanessa, Carolien Boeckx, Wim Wuyts, et al. "Abstract 2122:KRASmutation detection using high resolution melting analysis and its prognostic value in archival colorectal cancer tissues." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-2122.

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Sharma, Kusum, Aman Sharma, and Mandeep Dhillon. "SAT0471 HIGH-RESOLUTION MELTING CURVE ANALYSIS: A RAPID AND PRAGMATIC APPROACH FOR SCREENING OF MULTIDRUG RESISTANT OSTEOARTICULAR TUBERCULOSIS (OATB)." In Annual European Congress of Rheumatology, EULAR 2019, Madrid, 12–15 June 2019. BMJ Publishing Group Ltd and European League Against Rheumatism, 2019. http://dx.doi.org/10.1136/annrheumdis-2019-eular.6941.

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Schiza, Christina, Sofia Farkona, Maria Chimonidou, et al. "Abstract 2088: Detection ofPIK3CAsomatic mutations in cell-free DNA of breast cancer patients by high-resolution melting curve analysis." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-2088.

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Hashida, Shinsuke, Junichi Soh, Shinichi Toyooka, et al. "Abstract 4219: A novel high-sensitive assay for detection of EGFR T790M mutation using high resolution melting analysis with mutant-enriched COLD PCR." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4219.

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Звіти організацій з теми "High resolution melting analysi"

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Mu, Xuemeng, Hengyan Zhang, Mei Li, and Jia Zhang. Molecular diagnostic yield of next generation sequencing and high resolution melting analysisin osteogenesis imperfecta: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2024. http://dx.doi.org/10.37766/inplasy2024.1.0009.

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Berman, R. G., B. E. Taylor, W. J. Davis, M. Sanborn-Barrie, and J B Whalen. Crustal architecture and evolution of the central Thelon tectonic zone, Nunavut: insights from Sm-Nd and O isotope analysis, U-Pb zircon geochronology, and targeted bedrock mapping. Natural Resources Canada/CMSS/Information Management, 2024. http://dx.doi.org/10.4095/332497.

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Анотація:
New isotopic analyses (Sm-Nd, O, and U-Pb), targeted geological mapping, and previously published whole-rock geochemical data and high-resolution aeromagnetic surveys define ten crustal domains across the central Thelon tectonic zone. In the eastern Slave Craton, granitoid rocks in the Overby Lake domain are more isotopically evolved than in the Tinney Hills domain and include tonalite dated at 2.71 Ga. The 400 km long main leucogranite belt separates most early (ca. 2.07-1.95 Ga) Thelon tectonic zone plutonic belts from the Queen Maud Block. Oxygen isotopes support its formation via melting o
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3

Lever, James, Susan Taylor, Arnold Song, Zoe Courville, Ross Lieblappen, and Jason Weale. The mechanics of snow friction as revealed by micro-scale interface observations. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/42761.

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The mechanics of snow friction are central to competitive skiing, safe winter driving and efficient polar sleds. For nearly 80 years, prevailing theory has postulated that self-lubrication accounts for low kinetic friction on snow: dry-contact sliding warms snow grains to the melting point, and further sliding produces meltwater layers that lubricate the interface. We sought to verify that self-lubrication occurs at the grain scale and to quantify the evolution of real contact area to aid modeling. We used high-resolution (15 μm) infrared thermography to observe the warming of stationary snow
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