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Статті в журналах з теми "HPTLC-bioautography"

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Автор: Grafiati

Опубліковано: 10 травня 2022

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1

Favre-Godal, Quentin, Emerson Ferreira Queiroz, and Jean-Luc Wolfender. "Latest Developments in Assessing Antifungal Activity Using TLC-Bioautography: A Review." Journal of AOAC INTERNATIONAL 96, no. 6 (November 1, 2013): 1175–88. http://dx.doi.org/10.5740/jaoacint.sgefavre-godal.

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Abstract This paper reviews the use of TLC-bioautography in the search for antifungal compounds from natural sources. The main methods used for antifungal screening are presented, with special emphasis on bioautography. Different aspects of the technique, including the latest chromatographic developments such as HPTLC and HPLC microfractionation are presented. The present status and recent advances made in antifungal bioautography are discussed, and a comprehensive review of the applications over the last 6 years is presented. Various strategies applied in the search for antifungal compounds from natural sources are discussed, with a highlight on the challenges faced when screening complex crude mixtures. The activities of approximately 100 antifungal compounds of natural origin are presented with their minimum inhibitory quantity. The most active natural source compounds against Candida, Cladosporium, Colletotrichum, and Fusarium species are highlighted, and the compound activities discussed. In addition, perspectives concerning future improvements in bioautography sensitivity and reproducibility are noted.

2

Theiler, Barbara A., Stefanie Istvanits, Martin Zehl, Laurence Marcourt, Ernst Urban, Lugardo O. Espinoza Caisa та Sabine Glasl. "HPTLC Bioautography Guided Isolation of α-Glucosidase Inhibiting Compounds fromJusticia secundaVahl (Acanthaceae)". Phytochemical Analysis 28, № 2 (2 грудня 2016): 87–92. http://dx.doi.org/10.1002/pca.2651.

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3

Santosh S. Bhujbal, Bhushankumar G. Chawale, and Mayuri A. Kale. "Application based Studies of HPTLC-bioautography in Evaluation of Botanicals: a Review." Journal of Analytical Chemistry 77, no. 4 (April 2022): 473–83. http://dx.doi.org/10.1134/s1061934822040116.

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4

Parveen, Rabea, Nausheen Khan, Sultan Zahiruddin, Mohammad Ibrahim, Varisha Anjum, Bushra Parveen, and Mohammad Ahmad Khan. "TLC-Bioautographic Evaluation for High-Throughput Screening and Identification of Free Radical Scavenging and Antidiabetic Compounds from Traditional Unani Medicinal Plant: Citrullus colocynthis Schrad." Journal of AOAC INTERNATIONAL 103, no. 3 (May 2020): 669–77. http://dx.doi.org/10.5740/jaoacint.19-0287.

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Abstract Background: Interest in the antioxidant and antidiabetic activity of natural products are growing vastly in the modern world. Thin layer chromatography-bioautography-mass spectroscopy (TLC-bioautography-MS) plays an important role in chemico-biological screening of natural sources. TLC combined with 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical, α-amylase and α-glucosidase bioassay were used to evaluate antioxidant and antidiabetic activities, respectively, in different extracts of Citrullus colocynthis (Hanzal), a well-known traditional Indian Unani medicinal plant. Objective: To develop a TLC-bioautographic-MS method for DPPH, α-amylase, and glucosidase inhibitors in different extract of C. colocynthis fruits. Method: Fruits of C. colocynthis were successively extracted with toluene, dichloromethane, ethyl acetate, methanol, and water. TLC solvents were developed, and bioautographic-MS analysis was carried out to identify the antioxidant and antidiabetic compounds. Results: HPTLC fingerprinting analysis showed maximum numbers of band separated in dichloromethane and ethyl acetate extracts of C. colocynthis, fourteen and thirteen at 254 and 366 nm, respectively. Whereas six and five separated bands were observed in toluene extract at 254 and 366 nm, respectively showed minimum numbers of metabolites. Based on TLC-bioautography-MS, maximum number of antioxidant compounds were identified in dichloromethane extract. Except aqueous extract of C. colocynthis, all the extracts have shown antidiabetic activity. On the other hand, there were no antioxidant compounds in methanolic extract of C. colocynthis. Conclusions: The results of this study reveal that TLC-bioautography-MS–guided strategy used to identify antioxidant and antidiabetic compounds of C. colocynthis is very useful technique for high-throughput screening of bioactive compounds. Highlights: TLC-MS bioautography is a simple and fast to enables bioactive compounds present in extracts.

5

Chen, Yisheng, Caihong Huang, Zhengyu Jin, Xueming Xu, Yina Cai, and Yuxiang Bai. "HPTLC-bioautography/SERS screening nifedipine adulteration in food supplement based on Ginkgo biloba." Microchemical Journal 154 (May 2020): 104647. http://dx.doi.org/10.1016/j.microc.2020.104647.

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6

Hilaire, Valentin, Gregory Michel, Alissa Majoor, Francis Hadji-Minaglou, Anne Landreau, and Xavier Fernandez. "New method for screening anti-Leishmania compounds in plants extracts by HPTLC-bioautography." Journal of Chromatography B 1188 (January 2022): 123061. http://dx.doi.org/10.1016/j.jchromb.2021.123061.

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7

Ristivojević, Petar, Ivica Dimkić, Jelena Trifković, Tanja Berić, Irena Vovk, Dušanka Milojković-Opsenica, and Slaviša Stanković. "Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics." PLOS ONE 11, no. 6 (June 7, 2016): e0157097. http://dx.doi.org/10.1371/journal.pone.0157097.

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8

Adhami, Hamid-Reza, Uta Scherer, Hanspeter Kaehlig, Timm Hettich, Götz Schlotterbeck, Eike Reich, and Liselotte Krenn. "Combination of Bioautography with HPTLC-MS/NMR: A Fast Identification of Acetylcholinesterase Inhibitors from Galbanum†." Phytochemical Analysis 24, no. 4 (February 21, 2013): 395–400. http://dx.doi.org/10.1002/pca.2422.

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9

Stankovic, Jovana, Miroslav Novakovic, Vele Tesevic, Ana Ciric, Marina Sokovic, Gordana Zdunic, Zora Dajic-Stevanovic, and Dejan Godjevac. "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil." Journal of the Serbian Chemical Society 84, no. 12 (2019): 1355–65. http://dx.doi.org/10.2298/jsc190513106s.

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This study was performed to determine the main antibacterial compounds of the essential oil (??) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC?MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.

10

Zhang, Wan-Xin, In-Cheng Chao, De-jun Hu, Farid Shakerian, Liya Ge, Xiao Liang, Ying Wang, Jing Zhao, and Shao-Ping Li. "Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography." Journal of AOAC INTERNATIONAL 102, no. 3 (May 1, 2019): 726–33. http://dx.doi.org/10.5740/jaoacint.18-0307.

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Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

11

Altintas, Ayhan, Nurhayat Tabanca, Erno Tyihák, Péter G. Ott, Ágnes M. Móricz, Emil Mincsovics, and David E. Wedge. "Characterization of Volatile Constituents from Origanum onites and Their Antifungal and Antibacterial Activity." Journal of AOAC INTERNATIONAL 96, no. 6 (November 1, 2013): 1200–1208. http://dx.doi.org/10.5740/jaoacint.sgealtintas.

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Abstract Essential oils obtained by hydrodistillation (HD) and microwave-assisted HD (MWHD) of Origanum onites aerial parts were analyzed by GC and GC/MS. Thirty-one constituents representing 98.6% of the water-distilled oil and 52 constituents representing 99.6% of the microwave-distilled oil were identified. Carvacrol (76.8% HD and 79.2% MWHD) and thymol (4.7% HD and 4.4% MWHD) were characterized as major constituents in both essential oils. Separation of carvacrol and thymol was achieved by overpressured layer chromatography. HPTLC and TLC separations were also compared. Essential oils were evaluated for antifungal activity against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides using a direct overlay bioautography assay. Furthermore, main oil components carvacrol and thymol were then evaluated for antifungal activity; only carvacrol demonstrated nonselective antifungal activity against the three Colletotrichum species. Thymol and carvacrol were subsequently evaluated in a 96-well microdilution broth assay against Phomopsis obscurans, Fusarium oxysporum, three Colletotrichum species, and Botrytis cinerea. No activity was observed against any of the three Colletotrichum species at or below 30 μM. However, thymol demonstrated antifungal activity and produced 31.7% growth inhibition of P. obscurans at 120 h and 0.3 μM, whereas carvacrol appeared inactive. Thymol and carvacrol at 30 μM showed 51.5 and 36.9% growth inhibition of B. cinerea at 72 h. The mechanism of antibacterial activity was studied in a bioautography-based BioArena system. Thymol and carvacrol showed similar inhibition/killing effect against Bacillus subtilis soil bacteria; the action could be enhanced by the formaldehyde generator and transporter copper(II) ions and could be decreased in the presence of L-arginine, a formaldehyde capturer. Results indicated that Origanum essential oils and its major components thymol and carvacrol appear to generate antimicrobial activity through a mechanism of action where formaldehyde and its reaction products are produced.

12

Jesionek, Anna, Loretta Poblocka-Olech, Bozena Zabiegala, Adam Bucinski, Miroslawa Krauze-Baranowska, and Maria Luczkiewicz. "Validated HPTLC method for determination of ledol and alloaromadendrene in the essential oil fractions of Rhododendron tomentosum plants and in vitro cultures and bioautography for their activity screening." Journal of Chromatography B 1086 (June 2018): 63–72. http://dx.doi.org/10.1016/j.jchromb.2018.04.006.

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13

Ahmed, Atallah F., Zhi-Hong Wen, Ahmed H. Bakheit, Omer A. Basudan, Hazem A. Ghabbour, Abdullah Al-Ahmari, and Chien-Wei Feng. "A Major Diplotaxis harra-Derived Bioflavonoid Glycoside as a Protective Agent against Chemically Induced Neurotoxicity and Parkinson’s Models; In Silico Target Prediction; and Biphasic HPTLC-Based Quantification." Plants 11, no. 5 (February 27, 2022): 648. http://dx.doi.org/10.3390/plants11050648.

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Oxidative stress and chronic inflammation have a role in developing neurodegenerative diseases such as Parkinson’s disease (PD) and inflammatory movement disorders such as rheumatoid arthritis that affect millions of populations. In searching for antioxidant and anti-inflammatory molecules from natural sources that can counteract neurodegenerative diseases and arthritis, the flavonoid-rich extract of Diplotaxis harra (DHE) was selected based on its in vitro antioxidant and anti-inflammatory activities. DHE could inhibit the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions in the lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages from 100% to the level of 28.51 ± 18.67 and 30.19 ± 5.00% at 20 μg/mL, respectively. A TLC bioautography of DHE fractions using 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH) led to the isolation of a major antioxidant compound which was identified by X-ray diffraction analysis as isorhamnetin-3-O-β-D-glucoside (IR3G). IR3G also exhibited a potent anti-inflammatory activity, particularly by suppressing the upregulation of iNOS expression, similar to that of dexamethasone (DEX) at 10 μM to the level of 35.96 ± 7.80 and 29.34 ± 6.34%, respectively. Moreover, IR3G displayed a strong neuroprotectivity (>60% at 1.0−4–1.0−3 μM) against 6-hydroxydopamine (6-OHDA)-challenged SHSY5Y neuroblastoma, an in vitro model of dopaminergic neurons for Parkinson’s disease (PD) research. Accordingly, the in vivo anti-Parkinson potentiality was evaluated, where it was found that IR3G successfully reversed the 6-OHDA-induced locomotor deficit in a zebrafish model. A study of molecular docking and molecular dynamic (MD) simulation of IR3G and its aglycone isorhamnetin (IR) against human acetylcholine esterase (AChE), monoamine oxidase B (MAO-B), and Polo-like kinase-2 (PLK2) was performed and further outlined a putative mechanism in modulating neurodegenerative diseases such as PD. The free radical scavenging, anti-inflammatory through anti-iNOS and anti-COX-2 expression, and neuroprotective activities assessed in this study would present partial evidence for the potentiality of D. harra-derived IR3G as a promising natural therapeutic agent against neurodegenerative diseases and inflammatory arthritis. Finally, a biphasic HPTLC method was developed to estimate the biomarker IR3G in D. harra quantitatively.

14

Annegowda, H. V., P. Y. Tan, M. N. Mordi, S. Ramanathan, M. R. Hamdan, M. H. Sulaiman, and S. M. Mansor. "TLC–Bioautography-Guided Isolation, HPTLC and GC–MS-Assisted Analysis of Bioactives of Piper betle Leaf Extract Obtained from Various Extraction Techniques: In vitro Evaluation of Phenolic Content, Antioxidant and Antimicrobial Activities." Food Analytical Methods 6, no. 3 (August 7, 2012): 715–26. http://dx.doi.org/10.1007/s12161-012-9470-y.

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15

Wang, Yuan, Evgenia Glukhov, Yifan He, Yayue Liu, Longjian Zhou, Xiaoxiang Ma, Xueqiong Hu, Pengzhi Hong, William H. Gerwick, and Yi Zhang. "Secondary Metabolite Variation and Bioactivities of Two Marine Aspergillus Strains in Static Co-Culture Investigated by Molecular Network Analysis and Multiple Database Mining Based on LC-PDA-MS/MS." Antibiotics 11, no. 4 (April 12, 2022): 513. http://dx.doi.org/10.3390/antibiotics11040513.

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Co-culture is known as an efficient way to explore the metabolic potential of fungal strains for new antibiotics and other therapeutic agents that could counter emerging health issues. To study the effect of co-culture on the secondary metabolites and bioactivities of two marine strains, Aspergillus terreus C23-3 and Aspergillus. unguis DLEP2008001, they were co-cultured in live or inactivated forms successively or simultaneously. The mycelial morphology and high-performance thin layer chromatography (HPTLC) including bioautography of the fermentation extracts were recorded. Furthermore, the agar cup-plate method was used to compare the antimicrobial activity of the extracts. Based on the above, liquid chromatography-photodiode array-tandem mass spectrometry (LC-PDA-MS/MS) together with Global Natural Products Social molecular networking (GNPS) and multiple natural products database mining were used to further analyze their secondary metabolite variations. The comprehensive results showed the following trends: (1) The strain first inoculated will strongly inhibit the growth and metabolism of the latter inoculated one; (2) Autoclaved A. unguis exerted a strong inducing effect on later inoculated A. terreus, while the autoclaved A. terreus showed high stability of its metabolites and still potently suppressed the growth and metabolism of A. unguis; (3) When the two strains are inoculated simultaneously, they both grow and produce metabolites; however, the A. terreus seemed to be more strongly induced by live A. unguis and this inducing effect surpassed that of the autoclaved A. unguis. Under some of the conditions, the extracts showed higher antimicrobial activity than the axenic cultures. Totally, A. unguis was negative in response but potent in stimulating its rival while A. terreus had the opposite effect. Fifteen MS detectable and/or UV active peaks showed different yields in co-cultures vs. the corresponding axenic culture. GNPS analysis assisted by multiple natural products databases mining (PubChem, Dictionary of Natural Products, NPASS, etc.) gave reasonable annotations for some of these peaks, including antimicrobial compounds such as unguisin A, lovastatin, and nidulin. However, some of the peaks were correlated with antagonistic properties and remain as possible novel compounds without mass or UV matching hits from any database. It is intriguing that the two strains both synthesize chemical ‘weapons’ for antagonism, and that these are upregulated when needed in competitive co-culture environment. At the same time, compounds not useful in this antagonistic setting are downregulated in their expression. Some of the natural products produced during antagonism are unknown chlorinated metabolites and deserve further study for their antimicrobial properties. In summary, this study disclosed the different responses of two Aspergillus strains in co-culture, revealed their metabolic variation, and displayed new opportunities for antibiotic discovery.

16

P, GURUVAURAPPAN, MANAS RANJAN SAHOO, RAMESH RAGHAVA VARIER, ANITHAKUMARI RAJENDRAN, MAHESWARI HAREKRIHSN, RAMESH KARUPPASAMY, and SRIKRISHNA S. "PHYTOCHEMICAL PROFILING, HPTLC FINGERPRINT AND ANTIBACTERIAL, ANTI-FUNGAL, AND ANTIOXIDANT PROPERTIES OF ESSENTIAL OILS EXTRACTED FROM CUMMINUM CYMINUM, ZINGIBER OFFICINALE, TRACHYSPERMUM AMMI, ALIPNIA GALANGA, CEDRUS DEODARA, AND ELETTARIA CARDAMOMUM." Asian Journal of Pharmaceutical and Clinical Research, March 7, 2022, 50–55. http://dx.doi.org/10.22159/ajpcr.2022.v15i3.43737.

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Objective: The objective of the study was to carry out the phytochemical profiling of essential oils (EOs) and evaluation of their anti-microbial activity. Methods: The EOs extracted from Cumminum cyminum, Zingiber officinale, Trachyspermum ammi, Alipnia galanga, Cedrus deodara, and Elettaria cardamomum using clavenger apparatus. Phytochemical analysis and high-performance thin layer chromatography (HPTLC) fingerprinting were carried out for the EO. The antibacterial and antifungal activity were evaluated using agar well-diffusion method against two bacterial strains, Escherichia coli, Staphylococcus aureus and two fungal strains, Candida albicans, and Aspergillus brasiliensis. Positive controls ciprofloxacin-30 mg, azithromycin-15 mg, and nystatin NS-50 mg were used. Antioxidant potential of the EOs was investigated by TLC-bioautography method using 1,1-diphenyl-2-picrylhydrazyl derivatization. Results: The phytochemical analysis reveals presence of various phytochemical such as steroids, terpenoids, and phenylpropanoids. The HPTLC fingerprint is found to be unique for each of the oil. The EO of Z. officinale and T. ammi showed strong antibacterial activity against S. aureus. The EOs of C. cyminum, Tachyspermum ommi and A. galanga displayed prominent antioxidant activity on TLC bioautography. The herbs Cuminum cynimun, T. ammi, C. deodara, and Ellateria cardamomum produce reasonable amount of essentials oil, which can be explored for useful their industrial applications. Conclusions: These EOs can be explored further for their antimicrobial activity. The HPTLC analysis along with derivatization with suitable chromogenic reagents can be a rapid and simple tool for quality control of various EOs.

17

"PHYTOCHEMICAL SCREENING, HPTLC AND BIOAUTOGRAPHY OF ZANTHOXYLUM RHETSA (ROXB.) DC. EXTRACTS." International Journal of Biology, Pharmacy and Allied Sciences 10, no. 9 (September 1, 2021). http://dx.doi.org/10.31032/ijbpas/2021/10.9.5584.

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18

Adhami, HR, U. Scherer, H. Kaehlig, T. Hettich, G. Schlotterbeck, E. Reich, and L. Krenn. "Combination of bioautography with HPTLC-MS/NMR: A fast identification of AChE inhibitors from Galbanum." Planta Medica 78, no. 11 (July 2012). http://dx.doi.org/10.1055/s-0032-1321241.

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19

Boka, VI, A. Argyropoulou, A. Ankli, E. Reich, N. Aligiannis, and AL Skaltsounis. "HPTLC bioautography as a potent tool for the discovery of new tyrosinase inhibitors from Greek flora." Planta Medica 80, no. 16 (October 30, 2014). http://dx.doi.org/10.1055/s-0034-1394768.

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20

Darwish, Reham S., Eman Shawky, Hala M. Hammoda, and Fathallah M. Harraz. "Peroxidase inhibitory and antioxidant constituents from Juniperus L. species guided by HPTLC-bioautography and molecular docking studies." Natural Product Research, December 9, 2019, 1–5. http://dx.doi.org/10.1080/14786419.2019.1700249.

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21

Theiler, BA, S. Istvanits, M. Zehl, E. Urban, L. Marcourt, LO Espinoza Caisa та S. Glasl. "α-Glucosidase inhibiting compounds from Justicia secunda VAHL (Acanthaceae) – from HPTLC bioautography screening to isolation and structure elucidation". Planta Medica 81, № 16 (25 листопада 2015). http://dx.doi.org/10.1055/s-0035-1565319.

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22

Vaidyanathan, Lalitha, and T. Sivaswamy Lokeswari. "Compounds from Vernonia arborea Buch.-Ham. Inhibit Microbes that Impair Wound Healing." Journal of Pharmaceutical Research International, September 21, 2021, 103–13. http://dx.doi.org/10.9734/jpri/2021/v33i44b32655.

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Aims: To identify the antimicrobial potency of the leaf fractions of Vernonia arborea against selected wound microbes viz., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Stenotrophomonas maltophilia. Background: Wound healing is often delayed due to the presence of polymicrobial load, that have to be abolished to facilitate the healing process. A major class of antimicrobial phytocompound reported to occur in Vernonia arborea species include sesquiterpenes. Reports on the wound healing potency of V. arborea in wound models of Wistar rats however did not report antimicrobial activity of the aqueous or methanolic extracts. Methodology: The column fractions of the hexane leaf extract were tested against the selected strains by agar well diffusion assay and the zone of inhibition confirmed with TLC bioautography at specific Rf. The minimum inhibitory concentration (MIC) of the bioactive fractions was identified using resazurin microtiter assay (REMA) and the minimum bactericidal concentration (MBC) was determined. HPTLC quantification was also performed. Results: Out of the 30 pooled fractions, six showed antimicrobial potency against all the five tested wound microbes. The minimum inhibitory concentrations of these fractions were determined, ranging from 15.62 µg/mL to 500 µg/mL for the different microbes. Quantitative High-Performance Thin Layer Chromatography (HPTLC) revealed two compounds (a and b) in the bioactive fraction10 with yields of 633 mg (63%) and 97 mg (9.7%) per gram of the extract. Conclusion: The findings suggest the potential use of the bioactive compound in chronic infectious wound management therapy.

23

Wolfram, E., S. Bräm, K. Danova, and B. Meier. "Enzymatic bioautography on HPTLC: combined phytochemical and activity screening tool for quality assessment and in vitro cultivation bioprocess control for selected medicinal plants from the Balkan region." Planta Medica 81, no. 16 (November 25, 2015). http://dx.doi.org/10.1055/s-0035-1565330.

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24

Móricz, Á. M., P. G. Ott, D. Krüzselyi, M. Baglyas, and G. E. Morlock. "High-performance thin-layer chromatography–direct bioautography combined with chemometrics for the distinction of goldenrod species." JPC – Journal of Planar Chromatography – Modern TLC, April 12, 2022. http://dx.doi.org/10.1007/s00764-022-00159-3.

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AbstractThirteen root extract samples of four goldenrod (Solidago) species present in Europe were investigated by hyphenated high-performance thin-layer chromatography (HPTLC). Only S. virgaurea is native, whereas S. gigantea, S. canadensis, and S. graminifolia have been introduced from North America. The bioactive zones in the Aliivibrio fischeri bioautogram were identified as polyacetylenes, labdane diterpenes, or clerodane diterpenes by HPTLC coupled to high-resolution mass spectrometry, exploiting the two interfaces, heated electrospray ionization, and direct analysis in real time. Principal component analysis of the obtained bioprofiles enabled the discrimination of the Solidago species. Furthermore, chemometrics pointed to the discriminative components, the main bioactive markers of the species: Z,Z-matricaria ester from S. virgaurea, solidagenone from S. canadensis, solidagoic acid A, and a dialdehyde clerodane diterpene from S. gigantea, and Z-dehydromatricaria ester from S. graminifolia.